LOCUS       NM_001256378            4216 bp    mRNA    linear   PRI 21-SEP-2024
DEFINITION  Homo sapiens minichromosome maintenance complex binding protein
            (MCMBP), transcript variant 2, mRNA.
ACCESSION   NM_001256378
VERSION     NM_001256378.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4216)
  AUTHORS   Saito,Y., Santosa,V., Ishiguro,K.I. and Kanemaki,M.T.
  TITLE     MCMBP promotes the assembly of the MCM2-7 hetero-hexamer to ensure
            robust DNA replication in human cells
  JOURNAL   Elife 11, e77393 (2022)
   PUBMED   35438632
  REMARK    GeneRIF: MCMBP promotes the assembly of the MCM2-7 hetero-hexamer
            to ensure robust DNA replication in human cells.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4216)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   3  (bases 1 to 4216)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   4  (bases 1 to 4216)
  AUTHORS   Yang,X., Coulombe-Huntington,J., Kang,S., Sheynkman,G.M., Hao,T.,
            Richardson,A., Sun,S., Yang,F., Shen,Y.A., Murray,R.R., Spirohn,K.,
            Begg,B.E., Duran-Frigola,M., MacWilliams,A., Pevzner,S.J.,
            Zhong,Q., Wanamaker,S.A., Tam,S., Ghamsari,L., Sahni,N., Yi,S.,
            Rodriguez,M.D., Balcha,D., Tan,G., Costanzo,M., Andrews,B.,
            Boone,C., Zhou,X.J., Salehi-Ashtiani,K., Charloteaux,B., Chen,A.A.,
            Calderwood,M.A., Aloy,P., Roth,F.P., Hill,D.E., Iakoucheva,L.M.,
            Xia,Y. and Vidal,M.
  TITLE     Widespread Expansion of Protein Interaction Capabilities by
            Alternative Splicing
  JOURNAL   Cell 164 (4), 805-817 (2016)
   PUBMED   26871637
REFERENCE   5  (bases 1 to 4216)
  AUTHORS   Quimbaya,M., Raspe,E., Denecker,G., De Craene,B., Roelandt,R.,
            Declercq,W., Sagaert,X., De Veylder,L. and Berx,G.
  TITLE     Deregulation of the replisome factor MCMBP prompts oncogenesis in
            colorectal carcinomas through chromosomal instability
  JOURNAL   Neoplasia 16 (9), 694-709 (2014)
   PUBMED   25246271
  REMARK    GeneRIF: the MCMBP protein accumulates to high levels in cancer
            cells, whereas in normal proliferating tissue its abundance is low,
            indicating that MCMBP could be exploited as a novel diagnostic
            marker for this type of carcinoma.
REFERENCE   6  (bases 1 to 4216)
  AUTHORS   Nishiyama,A., Frappier,L. and Mechali,M.
  TITLE     MCM-BP regulates unloading of the MCM2-7 helicase in late S phase
  JOURNAL   Genes Dev 25 (2), 165-175 (2011)
   PUBMED   21196493
  REMARK    GeneRIF: MCM-BP silencing in human cells also delays MCM
            dissociation in late S phase
REFERENCE   7  (bases 1 to 4216)
  AUTHORS   Takahashi,N., Quimbaya,M., Schubert,V., Lammens,T., Vandepoele,K.,
            Schubert,I., Matsui,M., Inze,D., Berx,G. and De Veylder,L.
  TITLE     The MCM-binding protein ETG1 aids sister chromatid cohesion
            required for postreplicative homologous recombination repair
  JOURNAL   PLoS Genet 6 (1), e1000817 (2010)
   PUBMED   20090939
  REMARK    GeneRIF: Knockdown of the human ETG1 results in defective chromatid
            cohesion.
            Publication Status: Online-Only
REFERENCE   8  (bases 1 to 4216)
  AUTHORS   Sakwe,A.M., Nguyen,T., Athanasopoulos,V., Shire,K. and Frappier,L.
  TITLE     Identification and characterization of a novel component of the
            human minichromosome maintenance complex
  JOURNAL   Mol Cell Biol 27 (8), 3044-3055 (2007)
   PUBMED   17296731
  REMARK    GeneRIF: MCM-BP is conserved in multicellular eukaryotes and shares
            limited homology with MCM proteins. MCM-BP formed a complex with
            MCM3 to MCM7, which excluded MCM2.
REFERENCE   9  (bases 1 to 4216)
  AUTHORS   Beausoleil,S.A., Villen,J., Gerber,S.A., Rush,J. and Gygi,S.P.
  TITLE     A probability-based approach for high-throughput protein
            phosphorylation analysis and site localization
  JOURNAL   Nat Biotechnol 24 (10), 1285-1292 (2006)
   PUBMED   16964243
REFERENCE   10 (bases 1 to 4216)
  AUTHORS   Grupe,A., Li,Y., Rowland,C., Nowotny,P., Hinrichs,A.L., Smemo,S.,
            Kauwe,J.S., Maxwell,T.J., Cherny,S., Doil,L., Tacey,K., van
            Luchene,R., Myers,A., Wavrant-De Vrieze,F., Kaleem,M.,
            Hollingworth,P., Jehu,L., Foy,C., Archer,N., Hamilton,G.,
            Holmans,P., Morris,C.M., Catanese,J., Sninsky,J., White,T.J.,
            Powell,J., Hardy,J., O'Donovan,M., Lovestone,S., Jones,L.,
            Morris,J.C., Thal,L., Owen,M., Williams,J. and Goate,A.
  TITLE     A scan of chromosome 10 identifies a novel locus showing strong
            association with late-onset Alzheimer disease
  JOURNAL   Am J Hum Genet 78 (1), 78-88 (2006)
   PUBMED   16385451
  REMARK    GeneRIF: Observational study of gene-disease association. (HuGE
            Navigator)
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BC004183.2 and AC027672.12.
            
            On Feb 27, 2020 this sequence version replaced NM_001256378.1.
            
            Summary: This gene encodes a protein which is a component of the
            hexameric minichromosome maintenance (MCM) complex which regulates
            initiation and elongation of DNA. Multiple transcript variants
            encoding different isoforms have been found for this gene.
            [provided by RefSeq, Jan 2012].
            
            Transcript Variant: This variant (2) uses an alternate in-frame
            splice site in the 3' coding region compared to variant 1. The
            resulting protein (isoform 2) is shorter compared to isoform 1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR18074967.856279.1,
                                           SRR14038194.939795.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000369077.4/ ENSP00000358073.3
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2459              BC004183.2         7-2465
            2460-4216           AC027672.12        184359-186115
FEATURES             Location/Qualifiers
     source          1..4216
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="10"
                     /map="10q26.11"
     gene            1..4216
                     /gene="MCMBP"
                     /gene_synonym="C10orf119; MCM-BP"
                     /note="minichromosome maintenance complex binding protein"
                     /db_xref="GeneID:79892"
                     /db_xref="HGNC:HGNC:25782"
                     /db_xref="MIM:610909"
     exon            1..317
                     /gene="MCMBP"
                     /gene_synonym="C10orf119; MCM-BP"
                     /inference="alignment:Splign:2.1.0"
     CDS             260..2182
                     /gene="MCMBP"
                     /gene_synonym="C10orf119; MCM-BP"
                     /note="isoform 2 is encoded by transcript variant 2;
                     MCM-binding protein; mini-chromosome maintenance
                     complex-binding protein"
                     /codon_start=1
                     /product="mini-chromosome maintenance complex-binding
                     protein isoform 2"
                     /protein_id="NP_001243307.1"
                     /db_xref="CCDS:CCDS58099.1"
                     /db_xref="GeneID:79892"
                     /db_xref="HGNC:HGNC:25782"
                     /db_xref="MIM:610909"
                     /translation="MPCGEDWLSHPLGIVQGFFAQNGVNPDWEKKVIEYFKEKLKENN
                     APKWVPSLNEVPLHYLKPNSFVKFRCMIQDMFDPEFYMGVYETVNQNTKAHVLHFGKY
                     RDVAECGPQQELDLNSPRNTTLERQTFYCVPVPGESTWVKEAYVNANQARVSPSTSYT
                     PSRHKRSYEDDDDMDLQPNKQKDQHAGARQAGSVGGLQWCGEPKRLETEASTGQQLNS
                     LNLSSPFDLNFPLPGEKGPACLVKVYEDWDCFKVNDILELYGILSVDPVLSILNNDER
                     DASALLDPMECTDTAEEQRVHSPPASLVPRIHVILAQKLQHINPLLPACLNKEESKTF
                     VSSFMSELSPVRAELLGFLTHALLGDSLAAEYLILHLISTVYTRRDVLPLGKFTVNLS
                     GCPRNSTFTEHLYRIIQHLVPASFRLQMTIENMNHLKFIPHKDYTANRLVSGLLQLPS
                     NTSLVIDETLLEQGQLDTPGVHNVTALSNLITWQKVDYDFSYHQMEFPCNINVFITSE
                     GRSLLPADCQIHLQPQLIPPNMEEYMNSLLSAVLPSVLNKFRIYLTLLRFLEYSISDE
                     ITKAVEDDFVEMRKNDPQSITADDLHQLLVVARCLSLSAGQTTLSRERWLRAKQLESL
                     RRTRLQQQKCVNGNEL"
     exon            318..403
                     /gene="MCMBP"
                     /gene_synonym="C10orf119; MCM-BP"
                     /inference="alignment:Splign:2.1.0"
     exon            404..544
                     /gene="MCMBP"
                     /gene_synonym="C10orf119; MCM-BP"
                     /inference="alignment:Splign:2.1.0"
     exon            545..586
                     /gene="MCMBP"
                     /gene_synonym="C10orf119; MCM-BP"
                     /inference="alignment:Splign:2.1.0"
     exon            587..688
                     /gene="MCMBP"
                     /gene_synonym="C10orf119; MCM-BP"
                     /inference="alignment:Splign:2.1.0"
     exon            689..833
                     /gene="MCMBP"
                     /gene_synonym="C10orf119; MCM-BP"
                     /inference="alignment:Splign:2.1.0"
     exon            834..985
                     /gene="MCMBP"
                     /gene_synonym="C10orf119; MCM-BP"
                     /inference="alignment:Splign:2.1.0"
     exon            986..1086
                     /gene="MCMBP"
                     /gene_synonym="C10orf119; MCM-BP"
                     /inference="alignment:Splign:2.1.0"
     exon            1087..1259
                     /gene="MCMBP"
                     /gene_synonym="C10orf119; MCM-BP"
                     /inference="alignment:Splign:2.1.0"
     exon            1260..1383
                     /gene="MCMBP"
                     /gene_synonym="C10orf119; MCM-BP"
                     /inference="alignment:Splign:2.1.0"
     exon            1384..1501
                     /gene="MCMBP"
                     /gene_synonym="C10orf119; MCM-BP"
                     /inference="alignment:Splign:2.1.0"
     exon            1502..1667
                     /gene="MCMBP"
                     /gene_synonym="C10orf119; MCM-BP"
                     /inference="alignment:Splign:2.1.0"
     exon            1668..1801
                     /gene="MCMBP"
                     /gene_synonym="C10orf119; MCM-BP"
                     /inference="alignment:Splign:2.1.0"
     exon            1802..1966
                     /gene="MCMBP"
                     /gene_synonym="C10orf119; MCM-BP"
                     /inference="alignment:Splign:2.1.0"
     exon            1967..2055
                     /gene="MCMBP"
                     /gene_synonym="C10orf119; MCM-BP"
                     /inference="alignment:Splign:2.1.0"
     exon            2056..4216
                     /gene="MCMBP"
                     /gene_synonym="C10orf119; MCM-BP"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2438..2443
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MCMBP"
                     /gene_synonym="C10orf119; MCM-BP"
                     /note="hexamer: ATTAAA"
     polyA_site      2463
                     /gene="MCMBP"
                     /gene_synonym="C10orf119; MCM-BP"
                     /note="major polyA site"
     regulatory      3842..3847
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MCMBP"
                     /gene_synonym="C10orf119; MCM-BP"
                     /note="hexamer: AATAAA"
     polyA_site      3866
                     /gene="MCMBP"
                     /gene_synonym="C10orf119; MCM-BP"
                     /note="major polyA site"
     polyA_site      4201
                     /gene="MCMBP"
                     /gene_synonym="C10orf119; MCM-BP"
     polyA_site      4216
                     /gene="MCMBP"
                     /gene_synonym="C10orf119; MCM-BP"
ORIGIN      
        1 attgtgcgaa gtgaaggcta ggggcccgta cgcgcccgcc tgactgtcgc cagcagctcc
       61 tcggcggccc caccgcagcc gccgctccct gaggcgcggg aggcccgcgc cccgcggctc
      121 gctgtgcgtg ggagggcgcg agcgaacgcg ggcgaggagc ggccgagccg ctgaagagga
      181 gctgggcgcc ggccgcccgg ccgcgctcgg cccgcggatc gcctccgccc ggtcttcgcc
      241 ggccccggcc cctggcgaga tgccgtgtgg ggaggattgg ctcagccacc cgctgggaat
      301 cgtgcaggga ttcttcgccc aaaatggagt taatcctgac tgggagaaga aagtaattga
      361 gtattttaag gaaaagctga aggaaaataa tgctcctaag tgggtaccat cactgaacga
      421 agttcccctt cattatttga aacctaatag ttttgtgaaa tttcgttgca tgattcagga
      481 tatgtttgac cctgagtttt acatgggagt ttatgaaacg gttaaccaaa acacaaaagc
      541 acatgttctt cattttggaa aatatagaga tgtagcagag tgtgggcctc aacaagaact
      601 tgatttaaac tctccacgaa ataccacttt ggaaagacag actttctatt gtgttccggt
      661 gcctggggaa tctacgtggg taaaagaagc ctatgttaat gcaaaccaag ctcgagtcag
      721 tccctcaaca tcctacactc ctagtcgcca caagaggagt tatgaagatg atgacgatat
      781 ggacctacag cccaataagc agaaagacca acatgcaggt gccagacaag cagggagtgt
      841 tggtggtctt caatggtgtg gagagccaaa acgtttagaa actgaagctt ctactgggca
      901 acagctgaac tctctgaact tgtcttctcc ttttgatttg aattttccat tgccaggaga
      961 gaagggccct gcatgccttg tgaaggttta tgaagattgg gattgtttca aagtaaatga
     1021 cattcttgag ctatatggca tactgtctgt ggatcctgtg ctgagtatac tgaataatga
     1081 tgaaagggat gcctctgcac tgctggatcc gatggagtgc acagacacag cagaggagca
     1141 gagagtacac agtcctcctg cttcattagt gccgagaatt catgtgatct tagcccagaa
     1201 gttgcaacac atcaacccat tattgcctgc ctgccttaac aaagaggaga gcaaaacctt
     1261 tgtttcaagt ttcatgtccg aattgtctcc agtcagagca gaacttcttg ggttccttac
     1321 tcatgccctt ctgggggata gtttggctgc tgaatacctt atattacatc tcatctccac
     1381 agtatataca agaagagatg tccttccact aggaaaattt acagttaact tgagtggttg
     1441 cccacggaat agtaccttca cagaacactt gtatcgaatt attcaacatc ttgttccagc
     1501 atcttttcgt ctgcagatga ctatagagaa catgaaccat ttgaaattca ttccccacaa
     1561 agactacaca gccaatcgct tggtcagtgg gctcctccag ctgcccagca atacttccct
     1621 tgtaatcgat gagactctcc tggaacaggg gcagctggat accccaggtg ttcataatgt
     1681 gacagccctg agcaacctca taacgtggca gaaggtggat tatgacttca gctaccatca
     1741 gatggaattc ccctgcaata ttaacgtttt cattacttcg gaggggaggt cactcctccc
     1801 ggcagactgc cagattcact tacagcccca gctaattcca ccaaacatgg aggagtacat
     1861 gaacagcctt ctctcagcgg tgctgccttc cgtgctgaac aaattccgca tttatctaac
     1921 tcttttgaga ttcttggaat atagcatatc tgatgaaata accaaggcag ttgaagatga
     1981 ctttgtggaa atgcggaaga acgaccctca gagcatcact gctgatgatc ttcaccagct
     2041 gctcgtggtg gctcggtgtc tgtctctcag tgctggtcag acaacgctgt caagagaacg
     2101 atggctgaga gcaaagcagc tagagtcttt aagaagaacg aggcttcagc agcaaaaatg
     2161 tgtgaatgga aatgaacttt aaagatgtaa tacctatgaa gagtaatggg caaactgtag
     2221 ccacataatt gtaaaattca gatattcatt tataccacat tgttttatag gtaatttcta
     2281 tcacaaacca gtgacatttc ctgaaatcaa gcctggtaac acctgatgtt tatatgatat
     2341 tcagtaagga cttttacctt actgatttca tggagctttt gaagtttgtt ttataataat
     2401 tatataaatt agtaatgatg taaaaaaagt atttgatatt aaaagtttaa tattgataat
     2461 gttgctgatt gtaccatttc cttagcttca gctgagtcat aggccagact gttgaaatgc
     2521 tgaaatgaag aaggttgttg cagtttcaaa gtcagaggaa tcgtgcttcg gatttcttat
     2581 gttttctagt tctctgtttt tccagttcac agtgggttgg ggtgcattca gtagtccatc
     2641 tttggggaac ggaggcgtac ttgccattga ttcacatgac tacatgaaat tctgtactgt
     2701 catttcccag atgtttggcc acagaaactt tttcccactt aacatttgtt aacagcctgc
     2761 aaaactaaac ttgtacatgg cagtggttcc cagacttttg tattttatgg accggtagta
     2821 atatttccaa aaatctgggg tactataagg ttgccaattt accttgccaa gtaatccgaa
     2881 taaatcactg tattatcacc atttttttca taaaaggaaa ggacaatcta tctctgaata
     2941 agaggagtcc tttaaacgga atgaatgtgg cttttggggg caaaagaaac caagacacta
     3001 cattgtcttt attttctcct atcccagtgc atttgagaac catgcataag ggaatgctgt
     3061 gctacaaagc tgtgcccaaa tatgaaaaca aaataggaaa cttaaaaagc aataccccct
     3121 ttagaaagtt tttattttct taaatgtcat tgagttgctt tgattctatt ggatttttgg
     3181 cattttttat gggatcatca gttggttcca agtatgttag atcagctaac atctgctact
     3241 ccagtaacag cctcgtacaa ctgcaggtag gttttctcca gaccaattag ttttaataga
     3301 gcaaactaac aacagactgt agtagcatgg ttatggcaac cagaatcttc agaaaggtta
     3361 ggacattact ttttaagctg tcagtggtat caaataactt acctagttgg aggcagataa
     3421 aggatccctt acgttttttc ctataaggcc taaattgaaa ttgttaacca aggaaacagg
     3481 gtcagccttg aaaaatcaag gaattcattg tacctaataa ctgaagtaaa aataactagt
     3541 tgttcaactt ttcctaaact caaatctatt tttataaaca aatgtaaata atgtttatat
     3601 tagagttgaa ctggttttca tttttataac tggtagacta gaccttcctt aaacttttag
     3661 aaataaaatg aaggcttcac tggatttgtg aggataaaat acattttctt taattgtcct
     3721 agagcaaagt acattagtca ccatgtgttt tttgtgccaa tgtaaattgt aatttaccaa
     3781 agaaaaatac atacattgct tggtcttgca gaaaagttcc cttgaaagaa cctttccaat
     3841 aaataaaacg tcccaaatta gcagtacctt gggctgtttt tcatgagtaa gaagattcac
     3901 catcccatgt gatctgtgtg gaaaaagacc atgtcctctt ggtggaagac atgagagagc
     3961 tgaactgaag tggaggaggt ggtgcaagag ggaccttcct gctcaaggcc cgcccaggca
     4021 gcggaatgga gtgcagtgct tggctgcaga aaccctttgt ccctcaccta tatatacacg
     4081 gacagtcaag tttgttgctc taacgtaagg cacagcgtta atcctgtatg gccaggaaac
     4141 tgagtagact cctgtgtaac cctgtttgga actttgcctt cttaaaatga tttttcaaag
     4201 atctctttcg aactaa
//
