LOCUS       NM_032485               7486 bp    mRNA    linear   PRI 11-OCT-2024
DEFINITION  Homo sapiens minichromosome maintenance 8 homologous recombination
            repair factor (MCM8), transcript variant 1, mRNA.
ACCESSION   NM_032485
VERSION     NM_032485.6
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 7486)
  AUTHORS   Mishra,R., Kumar,N., Bargali,A. and Maich,G.
  TITLE     Understanding the novel MCM8 gene mutation: primary ovarian
            insufficiency and uterine hypoplasia in siblings
  JOURNAL   BMJ Case Rep 17 (7), e259433 (2024)
   PUBMED   39074937
  REMARK    GeneRIF: Understanding the novel MCM8 gene mutation: primary
            ovarian insufficiency and uterine hypoplasia in siblings.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7486)
  AUTHORS   Zhou,L., Le,M.N.U., Du,Y., Chen,X., Jin,M., Xiang,H., Xia,L.E.,
            Zhou,J., He,J. and Ning,Y.
  TITLE     A novel cancer-germline gene DAZL promotes progression and
            cisplatin resistance of non-small cell lung cancer by upregulating
            JAK2 and MCM8
  JOURNAL   Gene 916, 148449 (2024)
   PUBMED   38588931
  REMARK    GeneRIF: A novel cancer-germline gene DAZL promotes progression and
            cisplatin resistance of non-small cell lung cancer by upregulating
            JAK2 and MCM8.
REFERENCE   3  (bases 1 to 7486)
  AUTHORS   Ding,L., Sun,M., Sun,Y., Li,J., Zhang,Z., Dang,S., Zhang,J.,
            Yang,B., Dai,Y., Zhou,Q., Zhou,D., Li,E., Peng,S. and Li,G.
  TITLE     MCM8 promotes gastric cancer progression through RPS15A and
            predicts poor prognosis
  JOURNAL   Cancer Med 13 (13), e7424 (2024)
   PUBMED   38988047
  REMARK    GeneRIF: MCM8 promotes gastric cancer progression through RPS15A
            and predicts poor prognosis.
REFERENCE   4  (bases 1 to 7486)
  AUTHORS   Cao,L., Liu,H., Han,Z., Huang,C., Guo,C., Zhao,L., Gao,C., Xu,Y.,
            Wang,G., Feng,Z. and Li,S.
  TITLE     MCM8 promotes lung cancer progression through upregulating DNAJC10
  JOURNAL   J Cell Mol Med 28 (12), e18488 (2024)
   PUBMED   39031896
  REMARK    GeneRIF: MCM8 promotes lung cancer progression through upregulating
            DNAJC10.
REFERENCE   5  (bases 1 to 7486)
  AUTHORS   Acharya,A., Bret,H., Huang,J.W., Mutze,M., Gose,M., Kissling,V.M.,
            Seidel,R., Ciccia,A., Guerois,R. and Cejka,P.
  TITLE     Mechanism of DNA unwinding by MCM8-9 in complex with HROB
  JOURNAL   Nat Commun 15 (1), 3584 (2024)
   PUBMED   38678026
  REMARK    GeneRIF: Mechanism of DNA unwinding by MCM8-9 in complex with HROB.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 7486)
  AUTHORS   Stolk,L., Zhai,G., van Meurs,J.B., Verbiest,M.M., Visser,J.A.,
            Estrada,K., Rivadeneira,F., Williams,F.M., Cherkas,L., Deloukas,P.,
            Soranzo,N., de Keyzer,J.J., Pop,V.J., Lips,P., Lebrun,C.E., van der
            Schouw,Y.T., Grobbee,D.E., Witteman,J., Hofman,A., Pols,H.A.,
            Laven,J.S., Spector,T.D. and Uitterlinden,A.G.
  TITLE     Loci at chromosomes 13, 19 and 20 influence age at natural
            menopause
  JOURNAL   Nat Genet 41 (6), 645-647 (2009)
   PUBMED   19448619
  REMARK    GeneRIF: Observational study and genome-wide association study of
            gene-disease association. (HuGE Navigator)
REFERENCE   7  (bases 1 to 7486)
  AUTHORS   Kinoshita,Y., Johnson,E.M., Gordon,R.E., Negri-Bell,H., Evans,M.T.,
            Coolbaugh,J., Rosario-Peralta,Y., Samet,J., Slusser,E.,
            Birkenbach,M.P. and Daniel,D.C.
  TITLE     Colocalization of MCM8 and MCM7 with proteins involved in distinct
            aspects of DNA replication
  JOURNAL   Microsc Res Tech 71 (4), 288-297 (2008)
   PUBMED   18072282
  REMARK    GeneRIF: We find that MCM8, like MCM7, colocalizes on a specific
            DNA segment of the c-MYC replication initiation zone (c-MYC
            replicator) of DNA replications together with Cdc6 and cdk2, but
            differs with MCM7 in spatial relation to RPA70 during S phase.
REFERENCE   8  (bases 1 to 7486)
  AUTHORS   Volkening,M. and Hoffmann,I.
  TITLE     Involvement of human MCM8 in prereplication complex assembly by
            recruiting hcdc6 to chromatin
  JOURNAL   Mol Cell Biol 25 (4), 1560-1568 (2005)
   PUBMED   15684404
  REMARK    GeneRIF: MCM8 is a crucial component of the pre-RC and that the
            interaction between hMCM8 and hcdc6 is required for pre-RC
            assembly.
REFERENCE   9  (bases 1 to 7486)
  AUTHORS   Johnson,E.M., Kinoshita,Y. and Daniel,D.C.
  TITLE     A new member of the MCM protein family encoded by the human MCM8
            gene, located contrapodal to GCD10 at chromosome band 20p12.3-13
  JOURNAL   Nucleic Acids Res 31 (11), 2915-2925 (2003)
   PUBMED   12771218
  REMARK    GeneRIF: The MCM8 gene is located contrapodal to GCD10 at
            chromosome band 20p12.3-13.
REFERENCE   10 (bases 1 to 7486)
  AUTHORS   Gozuacik,D., Chami,M., Lagorce,D., Faivre,J., Murakami,Y., Poch,O.,
            Biermann,E., Knippers,R., Brechot,C. and Paterlini-Brechot,P.
  TITLE     Identification and functional characterization of a new member of
            the human Mcm protein family: hMcm8
  JOURNAL   Nucleic Acids Res 31 (2), 570-579 (2003)
   PUBMED   12527764
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AJ439063.1, AK297806.1,
            BC008830.2 and AL035461.11.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Jun 2, 2019 this sequence version replaced NM_032485.5.
            
            Summary: The protein encoded by this gene is one of the highly
            conserved mini-chromosome maintenance proteins (MCM) that are
            essential for the initiation of eukaryotic genome replication. The
            hexameric protein complex formed by the mini-chromosome maintenance
            proteins is a key component of the pre-replication complex and may
            be involved in the formation of replication forks and in the
            recruitment of other DNA replication related proteins. This protein
            contains the central domain that is conserved among the
            mini-chromosome maintenance proteins. The encoded protein may
            interact with other mini-chromosome maintenance proteins and play a
            role in DNA replication. This gene may be associated with length of
            reproductive lifespan and menopause. Alternatively spliced
            transcript variants encoding distinct isoforms have been described.
            [provided by RefSeq, Jul 2013].
            
            Transcript Variant: This variant (1) encodes isoform (1). Both
            variants 1 and 3 encode the same isoform.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AJ439063.1, SRR1803611.202498.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000610722.4/ ENSP00000478141.1
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-277               AJ439063.1         1-277
            278-280             AK297806.1         211-213
            281-1007            AJ439063.1         281-1007
            1008-3030           BC008830.2         318-2340
            3031-7486           AL035461.11        87785-92240
FEATURES             Location/Qualifiers
     source          1..7486
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="20"
                     /map="20p12.3"
     gene            1..7486
                     /gene="MCM8"
                     /gene_synonym="C20orf154; dJ967N21.5; POF10"
                     /note="minichromosome maintenance 8 homologous
                     recombination repair factor"
                     /db_xref="GeneID:84515"
                     /db_xref="HGNC:HGNC:16147"
                     /db_xref="MIM:608187"
     exon            1..372
                     /gene="MCM8"
                     /gene_synonym="C20orf154; dJ967N21.5; POF10"
                     /inference="alignment:Splign:2.1.0"
     exon            373..525
                     /gene="MCM8"
                     /gene_synonym="C20orf154; dJ967N21.5; POF10"
                     /inference="alignment:Splign:2.1.0"
     CDS             378..2900
                     /gene="MCM8"
                     /gene_synonym="C20orf154; dJ967N21.5; POF10"
                     /EC_number="3.6.4.12"
                     /note="isoform 1 is encoded by transcript variant 1; REC
                     homolog; DNA helicase MCM8; DNA replication licensing
                     factor MCM8; MCM8 minichromosome maintenance deficient 8;
                     minichromosome maintenance complex component 8"
                     /codon_start=1
                     /product="DNA helicase MCM8 isoform 1"
                     /protein_id="NP_115874.3"
                     /db_xref="CCDS:CCDS13094.1"
                     /db_xref="GeneID:84515"
                     /db_xref="HGNC:HGNC:16147"
                     /db_xref="MIM:608187"
                     /translation="MNGEYRGRGFGRGRFQSWKRGRGGGNFSGKWREREHRPDLSKTT
                     GKRTSEQTPQFLLSTKTPQSMQSTLDRFIPYKGWKLYFSEVYSDSSPLIEKIQAFEKF
                     FTRHIDLYDKDEIERKGSILVDFKELTEGGEVTNLIPDIATELRDAPEKTLACMGLAI
                     HQVLTKDLERHAAELQAQEGLSNDGETMVNVPHIHARVYNYEPLTQLKNVRANYYGKY
                     IALRGTVVRVSNIKPLCTKMAFLCAACGEIQSFPLPDGKYSLPTKCPVPVCRGRSFTA
                     LRSSPLTVTMDWQSIKIQELMSDDQREAGRIPRTIECELVHDLVDSCVPGDTVTITGI
                     VKVSNAEEGSRNKNDKCMFLLYIEANSISNSKGQKTKSSEDGCKHGMLMEFSLKDLYA
                     IQEIQAEENLFKLIVNSLCPVIFGHELVKAGLALALFGGSQKYADDKNRIPIRGDPHI
                     LVVGDPGLGKSQMLQAACNVAPRGVYVCGNTTTTSGLTVTLSKDSSSGDFALEAGALV
                     LGDQGICGIDEFDKMGNQHQALLEAMEQQSISLAKAGVVCSLPARTSIIAAANPVGGH
                     YNKAKTVSENLKMGSALLSRFDLVFILLDTPNEHHDHLLSEHVIAIRAGKQRTISSAT
                     VARMNSQDSNTSVLEVVSEKPLSERLKVVPGETIDPIPHQLLRKYIGYARQYVYPRLS
                     TEAARVLQDFYLELRKQSQRLNSSPITTRQLESLIRLTEARARLELREEATKEDAEDI
                     VEIMKYSMLGTYSDEFGNLDFERSQHGSGMSNRSTAKRFISALNNVAERTYNNIFQFH
                     QLRQIAKELNIQVADFENFIGSLNDQGYLLKKGPKVYQLQTM"
     exon            526..630
                     /gene="MCM8"
                     /gene_synonym="C20orf154; dJ967N21.5; POF10"
                     /inference="alignment:Splign:2.1.0"
     exon            631..713
                     /gene="MCM8"
                     /gene_synonym="C20orf154; dJ967N21.5; POF10"
                     /inference="alignment:Splign:2.1.0"
     exon            714..863
                     /gene="MCM8"
                     /gene_synonym="C20orf154; dJ967N21.5; POF10"
                     /inference="alignment:Splign:2.1.0"
     exon            864..967
                     /gene="MCM8"
                     /gene_synonym="C20orf154; dJ967N21.5; POF10"
                     /inference="alignment:Splign:2.1.0"
     exon            968..1166
                     /gene="MCM8"
                     /gene_synonym="C20orf154; dJ967N21.5; POF10"
                     /inference="alignment:Splign:2.1.0"
     exon            1167..1252
                     /gene="MCM8"
                     /gene_synonym="C20orf154; dJ967N21.5; POF10"
                     /inference="alignment:Splign:2.1.0"
     exon            1253..1404
                     /gene="MCM8"
                     /gene_synonym="C20orf154; dJ967N21.5; POF10"
                     /inference="alignment:Splign:2.1.0"
     exon            1405..1600
                     /gene="MCM8"
                     /gene_synonym="C20orf154; dJ967N21.5; POF10"
                     /inference="alignment:Splign:2.1.0"
     exon            1601..1631
                     /gene="MCM8"
                     /gene_synonym="C20orf154; dJ967N21.5; POF10"
                     /inference="alignment:Splign:2.1.0"
     exon            1632..1772
                     /gene="MCM8"
                     /gene_synonym="C20orf154; dJ967N21.5; POF10"
                     /inference="alignment:Splign:2.1.0"
     exon            1773..1914
                     /gene="MCM8"
                     /gene_synonym="C20orf154; dJ967N21.5; POF10"
                     /inference="alignment:Splign:2.1.0"
     exon            1915..2110
                     /gene="MCM8"
                     /gene_synonym="C20orf154; dJ967N21.5; POF10"
                     /inference="alignment:Splign:2.1.0"
     exon            2111..2330
                     /gene="MCM8"
                     /gene_synonym="C20orf154; dJ967N21.5; POF10"
                     /inference="alignment:Splign:2.1.0"
     exon            2331..2540
                     /gene="MCM8"
                     /gene_synonym="C20orf154; dJ967N21.5; POF10"
                     /inference="alignment:Splign:2.1.0"
     exon            2541..2617
                     /gene="MCM8"
                     /gene_synonym="C20orf154; dJ967N21.5; POF10"
                     /inference="alignment:Splign:2.1.0"
     exon            2618..2807
                     /gene="MCM8"
                     /gene_synonym="C20orf154; dJ967N21.5; POF10"
                     /inference="alignment:Splign:2.1.0"
     exon            2808..7486
                     /gene="MCM8"
                     /gene_synonym="C20orf154; dJ967N21.5; POF10"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3629..3634
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MCM8"
                     /gene_synonym="C20orf154; dJ967N21.5; POF10"
                     /note="hexamer: AATAAA"
     polyA_site      3656
                     /gene="MCM8"
                     /gene_synonym="C20orf154; dJ967N21.5; POF10"
                     /note="major polyA site"
     regulatory      3666..3671
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MCM8"
                     /gene_synonym="C20orf154; dJ967N21.5; POF10"
                     /note="hexamer: CATAAA"
     polyA_site      3696
                     /gene="MCM8"
                     /gene_synonym="C20orf154; dJ967N21.5; POF10"
                     /note="major polyA site"
     regulatory      4382..4387
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MCM8"
                     /gene_synonym="C20orf154; dJ967N21.5; POF10"
                     /note="hexamer: AATAAA"
     polyA_site      4403
                     /gene="MCM8"
                     /gene_synonym="C20orf154; dJ967N21.5; POF10"
                     /note="major polyA site"
     regulatory      6500..6505
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MCM8"
                     /gene_synonym="C20orf154; dJ967N21.5; POF10"
                     /note="hexamer: AATAAA"
     polyA_site      6526
                     /gene="MCM8"
                     /gene_synonym="C20orf154; dJ967N21.5; POF10"
     regulatory      7274..7279
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MCM8"
                     /gene_synonym="C20orf154; dJ967N21.5; POF10"
                     /note="hexamer: ATTATA"
     polyA_site      7300
                     /gene="MCM8"
                     /gene_synonym="C20orf154; dJ967N21.5; POF10"
ORIGIN      
        1 ctttgagcgg aagttggatc actgaagcgc caaaaaagaa tttaggggaa gacctgattt
       61 tcgcttgagg catttttgcg gcgctgtgcg ctacagacac cttctggaag ctgcggtggg
      121 gaaactgagt ttcccgagcc gttgagacag atggggttca gcaccgcgcg gggacgacag
      181 gaaagctgcg gctgaagaga actgcggact tggcaggagt cgcctgagga gctaagatcc
      241 cggagactct tagccccgtg gtgcttctct acgggaggga gggagggaga atccggccgg
      301 acagatctgc gcgtatcctg gagccggccc agttgtgaac taggagagct ttgggacctc
      361 tgtcccaagc aagagagatg aatggagagt atagaggcag aggatttgga cgaggaagat
      421 ttcaaagctg gaaaagggga agaggtggtg ggaacttctc aggaaaatgg agagaaagag
      481 aacacagacc tgatctgagt aaaaccacag gaaaacgtac ttctgaacaa accccacagt
      541 ttttgctttc aacaaagacc ccacagtcaa tgcagtcaac attggatcga ttcataccat
      601 ataaaggctg gaagctttat ttctctgaag tttacagcga tagctctcct ttgattgaga
      661 agattcaagc atttgaaaaa tttttcacaa ggcatattga tttgtatgac aaggatgaaa
      721 tagaaagaaa gggaagtatt ttggtagatt ttaaagaact gacagaaggt ggtgaagtaa
      781 ctaacttgat accagatata gcaactgaac taagagatgc acctgagaaa accttggctt
      841 gcatgggttt ggcaatacat caggtgttaa ctaaggacct tgaaaggcat gcagctgagt
      901 tacaagccca ggaaggattg tctaatgatg gagaaacaat ggtaaatgtg ccacatattc
      961 atgcaagggt gtacaactat gagcctttga cacagctcaa gaatgtcaga gcaaattact
     1021 atggaaaata cattgctcta agagggacag tggttcgtgt cagtaatata aagcctcttt
     1081 gcaccaagat ggcttttctt tgtgctgcat gtggagaaat tcagagcttt cctcttccag
     1141 atggaaaata cagtcttccc acaaagtgtc ctgtgcctgt gtgtcgaggc aggtcattta
     1201 ctgctctccg cagctctcct ctcacagtta cgatggactg gcagtcaatc aaaatccagg
     1261 aattgatgtc tgatgatcag agagaagcag gtcggattcc acgaacaata gaatgtgagc
     1321 ttgttcatga tcttgtggat agctgtgtcc cgggagacac agtgactatt actggaattg
     1381 tcaaagtctc aaatgcggaa gaaggttctc gaaataagaa tgacaagtgt atgttccttt
     1441 tgtatattga agcaaattct attagtaata gcaaaggaca gaaaacaaag agttctgagg
     1501 atgggtgtaa gcatggaatg ttgatggagt tctcacttaa agacctttat gccatccaag
     1561 agattcaagc tgaagaaaac ctgtttaaac tcattgtcaa ctcgctttgc cctgtcattt
     1621 ttggtcatga acttgttaaa gcaggtttgg cattagcact ctttggagga agccagaaat
     1681 acgcagatga caaaaacaga attccaattc ggggagaccc ccacatcctt gttgttggag
     1741 atccaggcct aggaaaaagt caaatgctac aggcagcgtg caatgttgcc ccacgtggcg
     1801 tgtatgtttg tggtaacacc acgaccacct ctggtctgac ggtaactctt tcaaaagata
     1861 gttcctctgg agattttgct ttggaagctg gtgccctggt acttggtgat caaggtattt
     1921 gtggaatcga tgaatttgat aagatgggga atcaacatca agccttgttg gaagccatgg
     1981 agcagcaaag tattagtctt gctaaggctg gtgtggtttg tagccttcct gcaagaactt
     2041 ccattattgc tgctgcaaat ccagttggag gacattacaa taaagccaaa acagtttctg
     2101 agaatttaaa aatggggagt gcactactat ccagatttga tttggtcttt atcctgttag
     2161 atactccaaa tgagcatcat gatcacttac tctctgaaca tgtgattgca ataagagctg
     2221 gaaagcagag aaccattagc agtgccacag tagctcgtat gaatagtcaa gattcaaata
     2281 cttccgtact tgaagtagtt tctgagaagc cattatcaga aagactaaag gtggttcctg
     2341 gagaaacaat agatcccatt ccccaccagc tattgagaaa gtacattggc tatgctcggc
     2401 agtatgtgta cccaaggcta tccacagaag ctgctcgagt tcttcaagat ttttaccttg
     2461 agctccggaa acagagccag aggttaaata gctcaccaat cactaccagg cagctggaat
     2521 ctttgattcg tctgacagag gcacgagcaa ggttggaatt gagagaggaa gcaaccaaag
     2581 aagacgctga ggatatagtg gaaattatga aatatagcat gctaggaact tactctgatg
     2641 aatttgggaa cctagatttt gagcgatccc agcatggttc tggaatgagc aacaggtcaa
     2701 cagcgaaaag atttatttct gctctcaaca acgttgctga aagaacttat aataatatat
     2761 ttcaatttca tcaacttcgg cagattgcca aagaactaaa cattcaggtt gctgattttg
     2821 aaaattttat tggatcacta aatgaccagg gttacctctt gaaaaaaggc ccaaaagttt
     2881 accagcttca aactatgtaa aaggacttca ccaagttagg gcctcctggg tttattgcag
     2941 attaaagcca tctcagtgaa gatatgcgtg cacgcacaga cagacagaca cacacacaca
     3001 cacacacaca cacacacaca cacacacaca cagtcaaata ctgttctctg aaaaatgatg
     3061 tcccaaaagt attataatag gaaaaaagca ttaaatataa taaactaatt taagaagtga
     3121 taaagtctcc agatgcagta gctcacactg taatcacagt gactcaggag gctgaggtga
     3181 gaggattcct tgaggccagg gttcgagacc aaccttgggc aacatagcaa gaccccattt
     3241 cttaaaaaaa aaaaaaaaaa atttaaactt agctgggtat ggtggcacat gcctatagtc
     3301 tcagctactt gtgaggctga ggcaggagga ttctttgagc ccaggagttt gaggttacag
     3361 tgagccacaa tcacaccaat cactgcactc cagcctgggc aataaagtaa ctcttgactc
     3421 aaaaaaataa aaaaaattgt agtggtagcc atgtgttaat tgttaaataa attctccaaa
     3481 gggctaaaag taaattactt ataaattttt tatagttgta tttttgacct gccttttata
     3541 tgtatgaata tttcatagtt ttgcatatca gatgtaggca tacagacaaa tacataaacc
     3601 aatgaatata ttacatattc tgtgttccaa taaaacttta tttatggaca ctaaaatttg
     3661 aatttcataa aattttccca tgtcaagaat acaaaatact tgagttttgt ttttagctat
     3721 ttaataatag gtctcattta ttccacaggc tgtagtttgt agtcttgctt gaaacaatag
     3781 aaacagactg attaagcagg agaagttttt tgaaagaatt ttgtttggct cacggaatta
     3841 ttagaaggca ggtgaaccag gagggtaagc ttccagcagc aatttgtaaa accatgcctt
     3901 agaattggac taaggaagaa gctgctgaca ctccactgcc acacagggca ctggaagaaa
     3961 gtgctgctgc ctccctgccc cacctttgcc acttctgcag caggaatagg tagaagaatg
     4021 cccccacccg caccggaaca gcaacaaaag gattctgcat gagatgcctc cctaaattgc
     4081 tgaattcaaa aaagaagttg catacaaaga catctgattg aaaaagggta tgttatatgc
     4141 ccctttcata ggctgctagg gagttttcct ggttctactt tcaggtggtg ggatcaataa
     4201 gaccagaatt tctcatatgt tgtgagagga ttcaaatgtt acagggttgc cagccaaact
     4261 atcaatcatg tataaatcca acaaacactt tgtaacatac aagaactcag gaaatgtgaa
     4321 ccattgttgg agaatctact aaaatacggc ttcccgcaaa cgaagatgaa tggaaaatgt
     4381 aaataaaaag aactggcagt gtatatcaga tgtttaacta taggaccaga actaagatgt
     4441 ggagactatt gccatagacc acaatgtaaa tttttaagtg aggaaggaaa aatcaggaat
     4501 caaaaggggc caggtgcagt ggctcacatc tataatccca gagctttggg agttcgaggc
     4561 aggaggatca cttgaagcca gttttgagac cagcctatgc aacacattga gaccctatct
     4621 ctacaaaaaa tagattagct gggcacggtg gtgcatgcct attgtcctac ctactgtgga
     4681 ggctgaagta ggaaatcact tgagcccgag agtttgaggt tacagtgagc tatgattata
     4741 ccactgcact ccagcctggg caagagagca agaccttgtc tcttaaagaa aaaaaaaaaa
     4801 aatggagaaa gtagatagta gtataaatat agtgaagtct ttaaaagcta ggagttaaaa
     4861 gatatttaaa gcaaataact caggcatggt ggtgtgtgcc tgtagtccca gccactaagg
     4921 aggctgaggc aggagaatca cttgaacccg ggaggcagag gttgcagtga gccaagatcg
     4981 taccactgca ctccagcctg ggcaacagag tgagactctt tctccaaact aaataagaga
     5041 tatttaaagc tgaaaaaaaa tctattaata gaatttaaaa catttcacaa aagtcaacac
     5101 ataaataata tcaaccaaaa taaagtggta gacagaagag aaaatatact aatttattgt
     5161 tcaaagagga aaataagtct caaaggttaa ggctattata taaatttatt tttaaaaaga
     5221 cataaaccta cattttcaga agaagaagtt ccatgtttcc ttagattatg tatttagcat
     5281 ccattaatca cagggccaaa ctaacaacaa aagttgttta gctttccctt acaatccagc
     5341 ttttatgggt gtgcaaacat cacattacag tccctccaac cacaaacccc aacatatagt
     5401 atttcacttc ctgcccaata gtgggtgtcc ccacaccttt caaccactgt agcaacaaag
     5461 ctctggccta ggcttttcac ttgcttcttt actgcctgac tgactttggt gtttcctcat
     5521 gtggcctggt gtggcatggc acgccctctt gggagatgaa agtaatcttc cataggcaat
     5581 tgtttctgtg tcaccattgc tgattaaaac aaatcctaag tacaaatgtc agaacagaaa
     5641 catgcaaagc aagaaaacat tacatcatga aagtttcttt ttttttcttt tttctttttt
     5701 tttttttttt gagacagagt tttgctcttt ttgctcaggc tggagtacag tggcgcgatc
     5761 ttggttcacc gcaacctccg cctcctgggt tcaagcgttt ctcctgcctc agcctcccaa
     5821 gtagctggga ttataggcat gtgccaccat ggctaatttt gtatttttag tagagacggg
     5881 gtttctccat gttggtgagg ctggttttta actcccgaac tcaggtgatt gcctgcctcg
     5941 gtctcccaaa aagtgccggg attacaggcg tgagccattg tgcccagcca aaagtttctt
     6001 tttcaagaga tggtggtctt gccatgttgt ccagtatgga cttgaactac tcagctcaag
     6061 cagtcctccc acctcagcct cctgaatagc tgagactaca ggcatgcacc actacacctg
     6121 gctgcgagat gaaattttaa gagatggggg tcttgttctg tcgcccaggc tggagtgcag
     6181 tgacatgatc aagccgtgat ctcctgggct caagcaatcc tcctacctca gcctcctgag
     6241 tagctggaac tacaggaatg caacaccatg cccagctaat tttgattttt agtagagatg
     6301 aggtcttcct ttgttgccca agctggtctc aaactccagg gcttcctgag tagctgagac
     6361 tacaggtgtg agccaccatg cccagcctcc tctcttttcc aatattgtgt taagtaaatt
     6421 gtattggtgg taatcaactt gatcatttca tttttaggat agagatatca aggtgagggt
     6481 ataactgaac gagaagagga ataaacacca ccctgtgatt gatggatctt tatagctctc
     6541 ctttgataaa gaagggaaat atcgtactta cattagcatg ttatagttgc tgttgttata
     6601 cagaagaata ttaaacaata ttcatatatt gtttatgaat gcattgaatt cataaacaat
     6661 ggtatggata ttgactagcc aaagaggtgg ataaatgtag atacaatgat acggtgattt
     6721 ggtattcagt gtccattctg ctctcctagt gtgccttcct ataatgcagg ggctggaagg
     6781 tgagaaaata accacatttc ccagactccc tttgcaggta gatttccaga tgtgaattgg
     6841 gttccactga tcagacaccc atgtgtgaga tttggaatgc agaactgagg agagagccat
     6901 cttcctcttg cttgtgttct ggtggcatgt gtggttgagg agacatttaa tttccctgca
     6961 gcagaattct tgcagtcagt tgttgaggca agtggcgtgg tggatgcaac cctgattctt
     7021 cacctttctc attattgtgg ggtagcagcg cctctggcaa gccagacaga cctgcagtgt
     7081 ttttctggga gttaattctg gagacccagt atggaaccca ctcctagccc ttgtgacaat
     7141 attgcaagca tccagttccc tgttttacag tccttttaaa tgtggctagc tacagtagtt
     7201 tgtgtttcct gcaactaatc actcatacat ctgtatctaa agcactccac ctgggtcatg
     7261 tactatactt aaaattataa ggcaatatta attcacaaaa ctacctactc gttttattta
     7321 accctcagaa gaactgaaaa caaaagctat accaggcttg ctctagagcg tgggcttggg
     7381 aatggaaagg cagagtgcac ctattgaatt ccatcatcag tttttctata ccatggagtt
     7441 acttaccatg tgtatacata acactgatta aaaattttaa aaaaaa
//
