LOCUS       NM_182746               4062 bp    mRNA    linear   PRI 24-OCT-2024
DEFINITION  Homo sapiens minichromosome maintenance complex component 4 (MCM4),
            transcript variant 2, mRNA.
ACCESSION   NM_182746
VERSION     NM_182746.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4062)
  AUTHORS   Liu,X., Zhang,F., Fan,Y., Qiu,C. and Wang,K.
  TITLE     MCM4 potentiates evasion of hepatocellular carcinoma from
            sorafenib-induced ferroptosis through Nrf2 signaling pathway
  JOURNAL   Int Immunopharmacol 142 (Pt A), 113107 (2024)
   PUBMED   39276458
  REMARK    GeneRIF: MCM4 potentiates evasion of hepatocellular carcinoma from
            sorafenib-induced ferroptosis through Nrf2 signaling pathway.
REFERENCE   2  (bases 1 to 4062)
  AUTHORS   Jubelin,C., Munoz-Garcia,J., Ollivier,E., Cochonneau,D.,
            Vallette,F., Heymann,M.F., Oliver,L. and Heymann,D.
  TITLE     Identification of MCM4 and PRKDC as new regulators of osteosarcoma
            cell dormancy based on 3D cell cultures
  JOURNAL   Biochim Biophys Acta Mol Cell Res 1871 (3), 119660 (2024)
   PUBMED   38216092
  REMARK    GeneRIF: Identification of MCM4 and PRKDC as new regulators of
            osteosarcoma cell dormancy based on 3D cell cultures.
REFERENCE   3  (bases 1 to 4062)
  AUTHORS   Katsuya,N., Ishikawa,A., Kido,A., Fukui,T., Kobayashi,G.,
            Sekino,Y., Uraoka,N., Babasaki,T., Yasui,W., Sentani,K. and Oue,N.
  TITLE     Minichromosome Maintenance 4 Is Associated with Cancer Stemness and
            Poor Survival of Patients with Gastric Cancer
  JOURNAL   Pathobiology 90 (3), 147-154 (2023)
   PUBMED   35830849
  REMARK    GeneRIF: Minichromosome Maintenance 4 Is Associated with Cancer
            Stemness and Poor Survival of Patients with Gastric Cancer.
REFERENCE   4  (bases 1 to 4062)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   5  (bases 1 to 4062)
  AUTHORS   Zhou,H., Jiang,L., Wang,G., Su,L., Hou,L. and Xue,X.
  TITLE     Identification of MCM4 as a Prognostic Marker of Hepatocellular
            Carcinoma
  JOURNAL   Biomed Res Int 2021, 7479326 (2021)
   PUBMED   34961841
  REMARK    GeneRIF: Identification of MCM4 as a Prognostic Marker of
            Hepatocellular Carcinoma.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 4062)
  AUTHORS   Ladenburger,E.M., Fackelmayer,F.O., Hameister,H. and Knippers,R.
  TITLE     MCM4 and PRKDC, human genes encoding proteins MCM4 and DNA-PKcs,
            are close neighbours located on chromosome 8q12-->q13
  JOURNAL   Cytogenet Cell Genet 77 (3-4), 268-270 (1997)
   PUBMED   9284934
REFERENCE   7  (bases 1 to 4062)
  AUTHORS   Ishimi,Y., Ichinose,S., Omori,A., Sato,K. and Kimura,H.
  TITLE     Binding of human minichromosome maintenance proteins with histone
            H3
  JOURNAL   J Biol Chem 271 (39), 24115-24122 (1996)
   PUBMED   8798650
REFERENCE   8  (bases 1 to 4062)
  AUTHORS   Schulte,D., Richter,A., Burkhart,R., Musahl,C. and Knippers,R.
  TITLE     Properties of the human nuclear protein p85Mcm. Expression, nuclear
            localization and interaction with other Mcm proteins
  JOURNAL   Eur J Biochem 235 (1-2), 144-151 (1996)
   PUBMED   8631321
REFERENCE   9  (bases 1 to 4062)
  AUTHORS   Musahl,C., Schulte,D., Burkhart,R. and Knippers,R.
  TITLE     A human homologue of the yeast replication protein Cdc21.
            Interactions with other Mcm proteins
  JOURNAL   Eur J Biochem 230 (3), 1096-1101 (1995)
   PUBMED   7601140
REFERENCE   10 (bases 1 to 4062)
  AUTHORS   Hu,B., Burkhart,R., Schulte,D., Musahl,C. and Knippers,R.
  TITLE     The P1 family: a new class of nuclear mammalian proteins related to
            the yeast Mcm replication proteins
  JOURNAL   Nucleic Acids Res 21 (23), 5289-5293 (1993)
   PUBMED   8265339
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC021236.10, AK022899.1,
            BC031061.1 and BQ689703.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Jun 25, 2018 this sequence version replaced NM_182746.2.
            
            Summary: The protein encoded by this gene is one of the highly
            conserved mini-chromosome maintenance proteins (MCM) that are
            essential for the initiation of eukaryotic genome replication. The
            hexameric protein complex formed by MCM proteins is a key component
            of the pre-replication complex (pre_RC) and may be involved in the
            formation of replication forks and in the recruitment of other DNA
            replication related proteins. The MCM complex consisting of this
            protein and MCM2, 6 and 7 proteins possesses DNA helicase activity,
            and may act as a DNA unwinding enzyme. The phosphorylation of this
            protein by CDC2 kinase reduces the DNA helicase activity and
            chromatin binding of the MCM complex. This gene is mapped to a
            region on the chromosome 8 head-to-head next to the PRKDC/DNA-PK, a
            DNA-activated protein kinase involved in the repair of DNA
            double-strand breaks. Alternatively spliced transcript variants
            encoding the same protein have been reported. [provided by RefSeq,
            Jul 2008].
            
            Transcript Variant: This variant (2) lacks a segment in the 5' UTR,
            when compared to variant 1. Both variants encode the same protein.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803611.250739.1,
                                           SRR1803616.36926.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000649973.1/ ENSP00000496964.1
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-33                AC021236.10        104405-104437       c
            34-1820             AK022899.1         1-1787
            1821-2035           BC031061.1         1832-2046
            2036-2191           BQ689703.1         679-834
            2192-2893           AK022899.1         2159-2860
            2894-4062           AC021236.10        87218-88386         c
FEATURES             Location/Qualifiers
     source          1..4062
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="8"
                     /map="8q11.21"
     gene            1..4062
                     /gene="MCM4"
                     /gene_synonym="CDC21; CDC54; hCdc21; IMD54; NKCD; NKGCD;
                     P1-CDC21"
                     /note="minichromosome maintenance complex component 4"
                     /db_xref="GeneID:4173"
                     /db_xref="HGNC:HGNC:6947"
                     /db_xref="MIM:602638"
     exon            1..74
                     /gene="MCM4"
                     /gene_synonym="CDC21; CDC54; hCdc21; IMD54; NKCD; NKGCD;
                     P1-CDC21"
                     /inference="alignment:Splign:2.1.0"
     exon            75..158
                     /gene="MCM4"
                     /gene_synonym="CDC21; CDC54; hCdc21; IMD54; NKCD; NKGCD;
                     P1-CDC21"
                     /inference="alignment:Splign:2.1.0"
     CDS             89..2680
                     /gene="MCM4"
                     /gene_synonym="CDC21; CDC54; hCdc21; IMD54; NKCD; NKGCD;
                     P1-CDC21"
                     /EC_number="3.6.4.12"
                     /note="minichromosome maintenance deficient 4; homolog of
                     S. pombe cell devision cycle 21"
                     /codon_start=1
                     /product="DNA replication licensing factor MCM4"
                     /protein_id="NP_877423.1"
                     /db_xref="CCDS:CCDS6143.1"
                     /db_xref="GeneID:4173"
                     /db_xref="HGNC:HGNC:6947"
                     /db_xref="MIM:602638"
                     /translation="MSSPASTPSRRGSRRGRATPAQTPRSEDARSSPSQRRRGEDSTS
                     TGELQPMPTSPGVDLQSPAAQDVLFSSPPQMHSSAIPLDFDVSSPLTYGTPSSRVEGT
                     PRSGVRGTPVRQRPDLGSAQKGLQVDLQSDGAAAEDIVASEQSLGQKLVIWGTDVNVA
                     ACKENFQRFLQRFIDPLAKEEENVGIDITEPLYMQRLGEINVIGEPFLNVNCEHIKSF
                     DKNLYRQLISYPQEVIPTFDMAVNEIFFDRYPDSILEHQIQVRPFNALKTKNMRNLNP
                     EDIDQLITISGMVIRTSQLIPEMQEAFFQCQVCAHTTRVEMDRGRIAEPSVCGRCHTT
                     HSMALIHNRSLFSDKQMIKLQESPEDMPAGQTPHTVILFAHNDLVDKVQPGDRVNVTG
                     IYRAVPIRVNPRVSNVKSVYKTHIDVIHYRKTDAKRLHGLDEEAEQKLFSEKRVELLK
                     ELSRKPDIYERLASALAPSIYEHEDIKKGILLQLFGGTRKDFSHTGRGKFRAEINILL
                     CGDPGTSKSQLLQYVYNLVPRGQYTSGKGSSAVGLTAYVMKDPETRQLVLQTGALVLS
                     DNGICCIDEFDKMNESTRSVLHEVMEQQTLSIAKAGIICQLNARTSVLAAANPIESQW
                     NPKKTTIENIQLPHTLLSRFDLIFLLLDPQDEAYDRRLAHHLVALYYQSEEQAEEELL
                     DMAVLKDYIAYAHSTIMPRLSEEASQALIEAYVDMRKIGSSRGMVSAYPRQLESLIRL
                     AEAHAKVRLSNKVEAIDVEEAKRLHREALKQSATDPRTGIVDISILTTGMSATSRKRK
                     EELAEALKKLILSKGKTPALKYQQLFEDIRGQSDIAITKDMFEEALRALADDDFLTVT
                     GKTVRLL"
     exon            159..323
                     /gene="MCM4"
                     /gene_synonym="CDC21; CDC54; hCdc21; IMD54; NKCD; NKGCD;
                     P1-CDC21"
                     /inference="alignment:Splign:2.1.0"
     exon            324..487
                     /gene="MCM4"
                     /gene_synonym="CDC21; CDC54; hCdc21; IMD54; NKCD; NKGCD;
                     P1-CDC21"
                     /inference="alignment:Splign:2.1.0"
     exon            488..589
                     /gene="MCM4"
                     /gene_synonym="CDC21; CDC54; hCdc21; IMD54; NKCD; NKGCD;
                     P1-CDC21"
                     /inference="alignment:Splign:2.1.0"
     exon            590..685
                     /gene="MCM4"
                     /gene_synonym="CDC21; CDC54; hCdc21; IMD54; NKCD; NKGCD;
                     P1-CDC21"
                     /inference="alignment:Splign:2.1.0"
     exon            686..781
                     /gene="MCM4"
                     /gene_synonym="CDC21; CDC54; hCdc21; IMD54; NKCD; NKGCD;
                     P1-CDC21"
                     /inference="alignment:Splign:2.1.0"
     exon            782..920
                     /gene="MCM4"
                     /gene_synonym="CDC21; CDC54; hCdc21; IMD54; NKCD; NKGCD;
                     P1-CDC21"
                     /inference="alignment:Splign:2.1.0"
     exon            921..1141
                     /gene="MCM4"
                     /gene_synonym="CDC21; CDC54; hCdc21; IMD54; NKCD; NKGCD;
                     P1-CDC21"
                     /inference="alignment:Splign:2.1.0"
     exon            1142..1262
                     /gene="MCM4"
                     /gene_synonym="CDC21; CDC54; hCdc21; IMD54; NKCD; NKGCD;
                     P1-CDC21"
                     /inference="alignment:Splign:2.1.0"
     exon            1263..1522
                     /gene="MCM4"
                     /gene_synonym="CDC21; CDC54; hCdc21; IMD54; NKCD; NKGCD;
                     P1-CDC21"
                     /inference="alignment:Splign:2.1.0"
     exon            1523..1888
                     /gene="MCM4"
                     /gene_synonym="CDC21; CDC54; hCdc21; IMD54; NKCD; NKGCD;
                     P1-CDC21"
                     /inference="alignment:Splign:2.1.0"
     exon            1889..2016
                     /gene="MCM4"
                     /gene_synonym="CDC21; CDC54; hCdc21; IMD54; NKCD; NKGCD;
                     P1-CDC21"
                     /inference="alignment:Splign:2.1.0"
     exon            2017..2224
                     /gene="MCM4"
                     /gene_synonym="CDC21; CDC54; hCdc21; IMD54; NKCD; NKGCD;
                     P1-CDC21"
                     /inference="alignment:Splign:2.1.0"
     exon            2225..2453
                     /gene="MCM4"
                     /gene_synonym="CDC21; CDC54; hCdc21; IMD54; NKCD; NKGCD;
                     P1-CDC21"
                     /inference="alignment:Splign:2.1.0"
     exon            2454..2587
                     /gene="MCM4"
                     /gene_synonym="CDC21; CDC54; hCdc21; IMD54; NKCD; NKGCD;
                     P1-CDC21"
                     /inference="alignment:Splign:2.1.0"
     exon            2588..4062
                     /gene="MCM4"
                     /gene_synonym="CDC21; CDC54; hCdc21; IMD54; NKCD; NKGCD;
                     P1-CDC21"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2800..2805
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MCM4"
                     /gene_synonym="CDC21; CDC54; hCdc21; IMD54; NKCD; NKGCD;
                     P1-CDC21"
                     /note="hexamer: CATAAA"
     polyA_site      2826
                     /gene="MCM4"
                     /gene_synonym="CDC21; CDC54; hCdc21; IMD54; NKCD; NKGCD;
                     P1-CDC21"
     regulatory      3810..3815
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MCM4"
                     /gene_synonym="CDC21; CDC54; hCdc21; IMD54; NKCD; NKGCD;
                     P1-CDC21"
                     /note="hexamer: AATAAA"
     polyA_site      3830
                     /gene="MCM4"
                     /gene_synonym="CDC21; CDC54; hCdc21; IMD54; NKCD; NKGCD;
                     P1-CDC21"
     regulatory      4041..4046
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MCM4"
                     /gene_synonym="CDC21; CDC54; hCdc21; IMD54; NKCD; NKGCD;
                     P1-CDC21"
                     /note="hexamer: AATAAT"
     polyA_site      4062
                     /gene="MCM4"
                     /gene_synonym="CDC21; CDC54; hCdc21; IMD54; NKCD; NKGCD;
                     P1-CDC21"
                     /note="major polyA site"
ORIGIN      
        1 gcggtttggg agcgctactc gccaggtgga ctcggagtcc gcgagcgtcg tcggcaagcg
       61 gccgcctttc cacggtactc cgagcactat gtcgtccccg gcgtcgaccc cgagccgccg
      121 cggcagccgg cgtggaaggg ccacccccgc ccagacgcct cggagtgagg atgccaggtc
      181 atctccctct cagagacgta gaggcgagga ttccacctcc acgggggagt tgcagccgat
      241 gccaacctcg cctggagtgg acctgcagag ccctgctgcg caggacgtgc tgttttccag
      301 ccctccccaa atgcattctt cagctatccc tcttgacttt gatgttagtt caccactgac
      361 atacggcact cccagctctc gggtagaggg aaccccaaga agtggtgtta ggggcacacc
      421 tgtgagacag aggcctgacc tgggctctgc acagaagggc ctgcaagtgg atctgcagtc
      481 tgacggggca gcagcagaag atatagtggc aagtgagcag tctctaggcc aaaaacttgt
      541 gatctgggga acagatgtaa atgtggcagc atgcaaagaa aactttcaga gatttcttca
      601 gcgttttatt gaccctctgg ctaaagaaga agaaaatgtt ggcatagata ttactgaacc
      661 tctatacatg caacgacttg gggagattaa tgttattggt gagccatttt taaatgtgaa
      721 ctgtgaacac atcaaatcat ttgacaaaaa tttgtacaga caactcatct cttacccaca
      781 ggaagttatt ccaacttttg acatggctgt caatgaaatc ttctttgacc gttaccctga
      841 ctcaatctta gaacatcaga ttcaagtaag accattcaac gcattgaaga ctaagaatat
      901 gagaaacctg aatccagaag acattgacca gctcatcacc atcagcggca tggtgatcag
      961 gacatcccag ctgattcccg agatgcagga ggccttcttc cagtgccaag tgtgtgccca
     1021 cacgacccgg gtggagatgg accgcggccg cattgcagag cccagtgtgt gcgggcgctg
     1081 ccacaccacc cacagcatgg cactcatcca caaccgctcc ctcttctctg acaagcagat
     1141 gatcaagctt caggagtctc cggaagacat gcctgcaggg cagacaccac acacagttat
     1201 cctgtttgct cacaatgatc tcgttgacaa ggtccagcct ggggacagag tgaatgttac
     1261 aggcatctat cgagctgtgc ctattcgagt caatccaaga gtgagtaatg tgaagtctgt
     1321 ctacaaaacc cacattgatg tcattcatta tcggaaaacg gatgcaaaac gtctgcatgg
     1381 ccttgatgaa gaagcagaac agaaactttt ttcagagaaa cgtgtggaat tgcttaagga
     1441 actttccagg aaaccagaca tttatgagag gcttgcttca gccttggctc caagcattta
     1501 tgaacatgaa gatataaaga agggaatttt gcttcagctc tttggcggga caaggaagga
     1561 ttttagtcac actggaaggg gcaaatttcg ggctgagatc aacatcttgc tgtgtggcga
     1621 ccctggtacc agcaagtccc agctgctgca gtacgtgtac aacctcgtcc ccaggggcca
     1681 gtacacgtct gggaagggct ccagtgcagt tggcctcact gcgtacgtaa tgaaagaccc
     1741 tgagacaagg cagctggtcc tgcagacagg tgctcttgtc ctgagtgaca acggcatctg
     1801 ctgtatcgat gagttcgaca agatgaatga aagtacaaga tcggtattgc atgaagtcat
     1861 ggaacagcag actctgtcca ttgcaaaggc tgggatcatc tgtcagctca atgcgcgcac
     1921 ctctgtcctg gcagcagcaa atcccattga gtctcagtgg aatcctaaaa aaacaaccat
     1981 tgaaaacatc cagctgcctc atactttatt atcaaggttt gatttgatct tcctcttgct
     2041 ggaccctcag gacgaagcct atgacaggcg tctggctcac cacctggtcg cactgtacta
     2101 ccagagcgag gagcaggcag aggaggagct cctggacatg gcggtgctaa aggactacat
     2161 tgcctacgcg cacagcacca tcatgccgcg gctaagtgag gaagccagcc aggctctcat
     2221 cgaggcttat gtagacatga ggaagattgg cagtagccgg ggaatggttt ctgcataccc
     2281 tcgacagcta gagtcattaa tccgcttagc agaagcccat gctaaagtaa gattgtctaa
     2341 caaagttgaa gccattgatg tggaagaggc caaacgcctc catcgggaag ctctgaagca
     2401 gtctgcaact gatccccgga ctggcatcgt ggacatatct attcttacta cggggatgag
     2461 tgccacctct cgtaaacgga aagaagaatt agctgaagca ttgaaaaagc ttattttatc
     2521 taagggcaaa acaccagctc taaaatacca gcaacttttt gaagatattc ggggacaatc
     2581 tgacatagca attactaaag atatgtttga agaagcactg cgtgccctgg cagatgatga
     2641 tttcctgaca gtgactggga agaccgtgcg cttgctctga agccttgtga gcaaggaagg
     2701 ctccctgcat gtcctgcttg ctgcacgcca catgggtgtg gtctgcatct cagttggccg
     2761 ccatcagtgt aaatagagct taaagtcatg gtttggctgc ataaaaattt tctaacttgg
     2821 gttcaatatt tgtagtgaag tatctgtttt catttttttc acgttataaa taaaaatact
     2881 atgctggccg ggcgcggtgg ctcacacctg taatcccagc actttgggag gccaatgtgg
     2941 gtggatcatg aggtcaggag ttcaagacca gcctagccaa gatggtgaaa ccccgtctct
     3001 agtaaagata acaaaaaatt agctgggctt gatggcatgc gcctgtaatc ccagctactc
     3061 gggaggttga ggcaggagaa tcgcttaaac ccaggcggca gaggttgcag tgagccaaga
     3121 tcgcgccact gcactccagc ctcagcaata gagtgagact gtctcaaaaa aaaaaaaaaa
     3181 aaaaaaacct gccaattttc aaacataccg tagagattat tttcaggtgc cattttatag
     3241 tatagcagca gggcttttac tctgtgtatg cacagatgca gtctggggca tggtttgtgt
     3301 gctggacttt ctcatggcca tcatcagtat gcttatggat ttgatgacag gcatagcctg
     3361 ggcatatcac ctcattggta aagggctaga gcctttcttt tttatggcac ttcttttttt
     3421 gagatagggt cttactctgt caccctggct agagtacact ggtacaatca cggctcaatg
     3481 taggcttaac ctcctgggct caggtgtatg tcactatgcc cggctacttt ttgtattttt
     3541 tggtagagac ggcttcgcca cgttgcccag gctgcaagcg atatgcctag gctcaagcga
     3601 tctgcccacc tcaacttccg gaagtgctga gattacaggt gtgagccact gcacccagcc
     3661 tttgttttat tttttatttt ttgagaggta tgattctttc tagagatttt ttctcatggc
     3721 tactattaga tcaggaatgg gtgattggag attattagat tctaggttaa cttctaccac
     3781 tttaccctaa tacataaaac tttttcctaa ataaatgatg gaaggaataa tacttggtta
     3841 cctggcatta tttttcagta agaaaaaagc tttactaacc actacattta tggaaatttg
     3901 taggggtaag tattttatag gtcataaaaa acaccataat ataacgaatc tcattttctt
     3961 taaatgtgaa ttaaatccta acagtcatct ttataaaatg accataggct aaaatcttac
     4021 gtgtaagtac tactacaata aataatttct gaaaccttta aa
//
