LOCUS       NM_002388               3068 bp    mRNA    linear   PRI 19-SEP-2024
DEFINITION  Homo sapiens minichromosome maintenance complex component 3 (MCM3),
            transcript variant 1, mRNA.
ACCESSION   NM_002388
VERSION     NM_002388.6
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3068)
  AUTHORS   Kang,E.Y., Millstein,J., Popovic,G., Meagher,N.S., Bolithon,A.,
            Talhouk,A., Chiu,D.S., Anglesio,M.S., Leung,B., Tang,K., Lambie,N.,
            Pavanello,M., Da-Anoy,A., Lambrechts,D., Loverix,L., Olbrecht,S.,
            Bisinotto,C., Garcia-Donas,J., Ruiz-Llorente,S.,
            Yague-Fernandez,M., Edwards,R.P., Elishaev,E., Olawaiye,A.,
            Taylor,S., Ataseven,B., du Bois,A., Harter,P., Lester,J.,
            Hogdall,C.K., Armasu,S.M., Huang,Y., Vierkant,R.A., Wang,C.,
            Winham,S.J., Heublein,S., Kommoss,F.K.F., Cramer,D.W., Sasamoto,N.,
            van-Wagensveld,L., Lycke,M., Mateoiu,C., Joseph,J., Pike,M.C.,
            Odunsi,K., Tseng,C.C., Pearce,C.L., Bilic,S., Conrads,T.P.,
            Hartmann,A., Hein,A., Jones,M.E., Leung,Y., Beckmann,M.W.,
            Ruebner,M., Schoemaker,M.J., Terry,K.L., El-Bahrawy,M.A.,
            Coulson,P., Etter,J.L., LaVigne-Mager,K., Andress,J., Grube,M.,
            Fischer,A., Neudeck,N., Robertson,G., Farrell,R., Barlow,E.,
            Quinn,C., Hettiaratchi,A., Casablanca,Y., Erber,R., Stewart,C.J.R.,
            Tan,A., Yu,Y., Boros,J., Brand,A.H., Harnett,P.R., Kennedy,C.J.,
            Nevins,N., Morgan,T., Fasching,P.A., Vergote,I., Swerdlow,A.J.,
            Candido Dos Reis,F.J., Maxwell,G.L., Neuhausen,S.L.,
            Barquin-Garcia,A., Modugno,F., Moysich,K.B., Crowe,P.J.,
            Hirasawa,A., Heitz,F., Karlan,B.Y., Goode,E.L., Sinn,P.,
            Horlings,H.M., Hogdall,E., Sundfeldt,K., Kommoss,S., Staebler,A.,
            Wu,A.H., Cohen,P.A., DeFazio,A., Lee,C.H., Steed,H., Le,N.D.,
            Gayther,S.A., Lawrenson,K., Pharoah,P.D.P., Konecny,G., Cook,L.S.,
            Ramus,S.J., Kelemen,L.E. and Kobel,M.
  TITLE     MCM3 is a novel proliferation marker associated with longer
            survival for patients with tubo-ovarian high-grade serous carcinoma
  JOURNAL   Virchows Arch 480 (4), 855-871 (2022)
   PUBMED   34782936
  REMARK    GeneRIF: MCM3 is a novel proliferation marker associated with
            longer survival for patients with tubo-ovarian high-grade serous
            carcinoma.
REFERENCE   2  (bases 1 to 3068)
  AUTHORS   Ma,H., Liu,Z., Li,H., Guo,X., Guo,S., Qu,P. and Wang,Y.
  TITLE     Bioinformatics Analysis Reveals MCM3 as an Important Prognostic
            Marker in Cervical Cancer
  JOURNAL   Comput Math Methods Med 2021, 8494260 (2021)
   PUBMED   34671420
  REMARK    GeneRIF: Bioinformatics Analysis Reveals MCM3 as an Important
            Prognostic Marker in Cervical Cancer.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3068)
  AUTHORS   Rubisz,P., Hirnle,L. and Kobierzycki,C.
  TITLE     The Immunohistochemical Expression of MCM-3, -5, and -7 Proteins in
            the Uterine Fibroids
  JOURNAL   Curr Issues Mol Biol 43 (2), 802-817 (2021)
   PUBMED   34449552
  REMARK    GeneRIF: The Immunohistochemical Expression of MCM-3, -5, and -7
            Proteins in the Uterine Fibroids.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 3068)
  AUTHORS   Qiu,X., Yu,H., Wu,H., Hu,Z., Zhou,J., Lin,H., Xue,W., Cai,W.,
            Chen,J., Yan,Q., Dai,W., Yang,M., Tang,D. and Dai,Y.
  TITLE     Single-cell chromatin accessibility landscape of human umbilical
            cord blood in trisomy 18 syndrome
  JOURNAL   Hum Genomics 15 (1), 40 (2021)
   PUBMED   34193281
  REMARK    GeneRIF: Single-cell chromatin accessibility landscape of human
            umbilical cord blood in trisomy 18 syndrome.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 3068)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   6  (bases 1 to 3068)
  AUTHORS   Musahl,C., Schulte,D., Burkhart,R. and Knippers,R.
  TITLE     A human homologue of the yeast replication protein Cdc21.
            Interactions with other Mcm proteins
  JOURNAL   Eur J Biochem 230 (3), 1096-1101 (1995)
   PUBMED   7601140
REFERENCE   7  (bases 1 to 3068)
  AUTHORS   Schulte,D., Burkhart,R., Musahl,C., Hu,B., Schlatterer,C.,
            Hameister,H. and Knippers,R.
  TITLE     Expression, phosphorylation and nuclear localization of the human
            P1 protein, a homologue of the yeast Mcm 3 replication protein
  JOURNAL   J Cell Sci 108 (Pt 4), 1381-1389 (1995)
   PUBMED   7615659
REFERENCE   8  (bases 1 to 3068)
  AUTHORS   Burkhart,R., Schulte,D., Hu,D., Musahl,C., Gohring,F. and
            Knippers,R.
  TITLE     Interactions of human nuclear proteins P1Mcm3 and P1Cdc46
  JOURNAL   Eur J Biochem 228 (2), 431-438 (1995)
   PUBMED   7705359
REFERENCE   9  (bases 1 to 3068)
  AUTHORS   Starborg,M., Brundell,E., Gell,K., Larsson,C., White,I.,
            Daneholt,B. and Hoog,C.
  TITLE     A murine replication protein accumulates temporarily in the
            heterochromatic regions of nuclei prior to initiation of DNA
            replication
  JOURNAL   J Cell Sci 108 (Pt 3), 927-934 (1995)
   PUBMED   7622621
REFERENCE   10 (bases 1 to 3068)
  AUTHORS   Thommes,P., Fett,R., Schray,B., Burkhart,R., Barnes,M., Kennedy,C.,
            Brown,N.C. and Knippers,R.
  TITLE     Properties of the nuclear P1 protein, a mammalian homologue of the
            yeast Mcm3 replication protein
  JOURNAL   Nucleic Acids Res 20 (5), 1069-1074 (1992)
   PUBMED   1549468
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL034343.17.
            
            On Nov 24, 2018 this sequence version replaced NM_002388.5.
            
            Summary: The protein encoded by this gene is one of the highly
            conserved mini-chromosome maintenance proteins (MCM) that are
            involved in the initiation of eukaryotic genome replication. The
            hexameric protein complex formed by MCM proteins is a key component
            of the pre-replication complex (pre_RC) and may be involved in the
            formation of replication forks and in the recruitment of other DNA
            replication related proteins. This protein is a subunit of the
            protein complex that consists of MCM2-7. It has been shown to
            interact directly with MCM5/CDC46. This protein also interacts with
            and is acetylated by MCM3AP, a chromatin-associated
            acetyltransferase. The acetylation of this protein inhibits the
            initiation of DNA replication and cell cycle progression. Several
            transcript variants encoding different isoforms have been found for
            this gene. [provided by RefSeq, Oct 2018].
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC001626.1, AY032603.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000596288.7/ ENSP00000472940.2
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-146               AL034343.17        63476-63621
            147-259             AL034343.17        64812-64924
            260-468             AL034343.17        65357-65565
            469-599             AL034343.17        66043-66173
            600-838             AL034343.17        68619-68857
            839-947             AL034343.17        69368-69476
            948-1101            AL034343.17        70530-70683
            1102-1233           AL034343.17        71020-71151
            1234-1442           AL034343.17        71742-71950
            1443-1617           AL034343.17        74302-74476
            1618-1744           AL034343.17        74862-74988
            1745-1895           AL034343.17        75767-75917
            1896-2036           AL034343.17        78992-79132
            2037-2140           AL034343.17        80250-80353
            2141-2226           AL034343.17        81522-81607
            2227-2296           AL034343.17        82074-82143
            2297-3068           AL034343.17        83432-84203
FEATURES             Location/Qualifiers
     source          1..3068
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="6"
                     /map="6p12.2"
     gene            1..3068
                     /gene="MCM3"
                     /gene_synonym="HCC5; P1-MCM3; P1.h; RLFB"
                     /note="minichromosome maintenance complex component 3"
                     /db_xref="GeneID:4172"
                     /db_xref="HGNC:HGNC:6945"
                     /db_xref="MIM:602693"
     exon            1..146
                     /gene="MCM3"
                     /gene_synonym="HCC5; P1-MCM3; P1.h; RLFB"
                     /inference="alignment:Splign:2.1.0"
     CDS             69..2495
                     /gene="MCM3"
                     /gene_synonym="HCC5; P1-MCM3; P1.h; RLFB"
                     /EC_number="3.6.4.12"
                     /note="isoform 1 is encoded by transcript variant 1;
                     minichromosome maintenance deficient 3; cervical cancer
                     proto-oncogene 5; hRlf beta subunit; replication licensing
                     factor, beta subunit; DNA polymerase alpha
                     holoenzyme-associated protein P1; DNA replication factor
                     MCM3; DNA replication licensing factor MCM3; p102; RLF
                     subunit beta; MCM3 minichromosome maintenance deficient 3"
                     /codon_start=1
                     /product="DNA replication licensing factor MCM3 isoform 1"
                     /protein_id="NP_002379.4"
                     /db_xref="CCDS:CCDS4940.3"
                     /db_xref="GeneID:4172"
                     /db_xref="HGNC:HGNC:6945"
                     /db_xref="MIM:602693"
                     /translation="MAGTVVLDDVELREAQRDYLDFLDDEEDQGIYQSKVRELISDNQ
                     YRLIVNVNDLRRKNEKRANRLLNNAFEELVAFQRALKDFVASIDATYAKQYEEFYVGL
                     EGSFGSKHVSPRTLTSCFLSCVVCVEGIVTKCSLVRPKVVRSVHYCPATKKTIERRYS
                     DLTTLVAFPSSSVYPTKDEENNPLETEYGLSVYKDHQTITIQEMPEKAPAGQLPRSVD
                     VILDDDLVDKAKPGDRVQVVGTYRCLPGKKGGYTSGTFRTVLIACNVKQMSKDAQPSF
                     SAEDIAKIKKFSKTRSKDIFDQLAKSLAPSIHGHDYVKKAILCLLLGGVERDLENGSH
                     IRGDINILLIGDPSVAKSQLLRYVLCTAPRAIPTTGRGSSGVGLTAAVTTDQETGERR
                     LEAGAMVLADRGVVCIDEFDKMSDMDRTAIHEVMEQGRVTIAKAGIHARLNARCSVLA
                     AANPVYGRYDQYKTPMENIGLQDSLLSRFDLLFIMLDQMDPEQDREISDHVLRMHRYR
                     APGEQDGDAMPLGSAVDILATDDPNFSQEDQQDTQIYEKHDNLLHGTKKKKEKMVSAA
                     FMKKYIHVAKIIKPVLTQESATYIAEEYSRLRSQDSMSSDTARTSPVTARTLETLIRL
                     ATAHAKARMSKTVDLQDAEEAVELVQYAYFKKVLEKEKKRKKRSEDESETEDEEEKSQ
                     EDQEQKRKRRKTRQPDAKDGDSYDPYDFSDTEEEMPQVHTPKTADSQETKESQKVELS
                     ESRLKAFKVALLDVFREAHAQSIGMNRLTESINRDSEEPFSSVEIQAALSKMQDDNQV
                     MVSEGIIFLI"
     exon            147..259
                     /gene="MCM3"
                     /gene_synonym="HCC5; P1-MCM3; P1.h; RLFB"
                     /inference="alignment:Splign:2.1.0"
     exon            260..468
                     /gene="MCM3"
                     /gene_synonym="HCC5; P1-MCM3; P1.h; RLFB"
                     /inference="alignment:Splign:2.1.0"
     exon            469..599
                     /gene="MCM3"
                     /gene_synonym="HCC5; P1-MCM3; P1.h; RLFB"
                     /inference="alignment:Splign:2.1.0"
     exon            600..838
                     /gene="MCM3"
                     /gene_synonym="HCC5; P1-MCM3; P1.h; RLFB"
                     /inference="alignment:Splign:2.1.0"
     exon            839..947
                     /gene="MCM3"
                     /gene_synonym="HCC5; P1-MCM3; P1.h; RLFB"
                     /inference="alignment:Splign:2.1.0"
     exon            948..1101
                     /gene="MCM3"
                     /gene_synonym="HCC5; P1-MCM3; P1.h; RLFB"
                     /inference="alignment:Splign:2.1.0"
     exon            1102..1233
                     /gene="MCM3"
                     /gene_synonym="HCC5; P1-MCM3; P1.h; RLFB"
                     /inference="alignment:Splign:2.1.0"
     exon            1234..1442
                     /gene="MCM3"
                     /gene_synonym="HCC5; P1-MCM3; P1.h; RLFB"
                     /inference="alignment:Splign:2.1.0"
     exon            1443..1617
                     /gene="MCM3"
                     /gene_synonym="HCC5; P1-MCM3; P1.h; RLFB"
                     /inference="alignment:Splign:2.1.0"
     exon            1618..1744
                     /gene="MCM3"
                     /gene_synonym="HCC5; P1-MCM3; P1.h; RLFB"
                     /inference="alignment:Splign:2.1.0"
     exon            1745..1895
                     /gene="MCM3"
                     /gene_synonym="HCC5; P1-MCM3; P1.h; RLFB"
                     /inference="alignment:Splign:2.1.0"
     exon            1896..2036
                     /gene="MCM3"
                     /gene_synonym="HCC5; P1-MCM3; P1.h; RLFB"
                     /inference="alignment:Splign:2.1.0"
     exon            2037..2140
                     /gene="MCM3"
                     /gene_synonym="HCC5; P1-MCM3; P1.h; RLFB"
                     /inference="alignment:Splign:2.1.0"
     exon            2141..2226
                     /gene="MCM3"
                     /gene_synonym="HCC5; P1-MCM3; P1.h; RLFB"
                     /inference="alignment:Splign:2.1.0"
     exon            2227..2296
                     /gene="MCM3"
                     /gene_synonym="HCC5; P1-MCM3; P1.h; RLFB"
                     /inference="alignment:Splign:2.1.0"
     exon            2297..3068
                     /gene="MCM3"
                     /gene_synonym="HCC5; P1-MCM3; P1.h; RLFB"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3041..3046
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MCM3"
                     /gene_synonym="HCC5; P1-MCM3; P1.h; RLFB"
                     /note="hexamer: AATAAA"
     polyA_site      3068
                     /gene="MCM3"
                     /gene_synonym="HCC5; P1-MCM3; P1.h; RLFB"
                     /note="major polyA site"
ORIGIN      
        1 gcgccggggt ggagtcatcc tgggaacctc cacgcgactt tggtggaggt agttctttgg
       61 cagcgggcat ggcgggtacc gtggtgctgg acgatgtgga gctgcgggag gctcagagag
      121 attacctgga cttcctggac gacgaggaag accagggaat ttatcagagc aaagttcggg
      181 agctgatcag tgacaaccaa taccggctga ttgtcaatgt gaatgacctg cgcaggaaaa
      241 acgagaagag ggctaaccgg cttctgaaca atgcctttga ggagctggtt gccttccagc
      301 gggccttaaa ggattttgtg gcctccattg atgctaccta tgccaagcag tatgaggagt
      361 tctacgtagg actggaaggc agctttggct ccaagcacgt ctccccgcgg actcttacct
      421 cctgcttcct cagctgtgtg gtctgtgtgg agggcattgt cactaaatgt tctctagttc
      481 gtcccaaagt cgtccgcagt gtccactact gtcctgctac taagaagacc atagagcgac
      541 gttattctga tctcaccacc ctggtggcct ttccctccag ctctgtctat cctaccaagg
      601 atgaggagaa caatcccctt gagacagaat atggcctttc tgtctacaag gatcaccaga
      661 ccatcaccat ccaggagatg ccggagaagg ccccagccgg ccagctcccc cgctctgtgg
      721 acgtcattct ggatgatgac ttggtggata aagcgaagcc tggtgaccgg gttcaggtgg
      781 tgggaaccta ccgttgcctt cctggaaaga agggaggcta cacctctggg accttcagga
      841 ctgtcctgat tgcctgtaat gttaagcaga tgagcaagga tgctcagccc tctttctctg
      901 ctgaggatat agccaagatc aagaagttca gtaaaacccg atccaaggat atctttgacc
      961 agctggccaa gtcattggcc ccaagtatcc atgggcatga ctatgtcaag aaagcaatcc
     1021 tctgcttgct cttgggaggg gtggaacgag acctagaaaa tggcagccac atccgtgggg
     1081 acatcaatat tcttctaata ggagacccat ccgttgccaa gtctcagctt ctgcggtatg
     1141 tgctttgcac tgcaccccga gctatcccca ccactggccg gggctcctct ggagtgggtc
     1201 tgacggctgc tgtcaccaca gaccaggaaa caggagagcg ccgtctggaa gcaggggcca
     1261 tggtcctggc tgaccgaggc gtggtttgca ttgatgaatt tgacaaaatg tctgacatgg
     1321 atcgcacagc catccatgaa gtgatggagc agggtcgagt gaccattgcc aaggctggca
     1381 tccatgctcg gctgaatgcc cgctgcagtg ttttggcagc tgccaaccct gtctacggca
     1441 ggtatgacca gtataagact ccaatggaga acattgggct acaggactca ctgctgtcac
     1501 gatttgactt gctcttcatc atgctggatc agatggatcc tgagcaggat cgggagatct
     1561 cagaccatgt ccttcggatg caccgttaca gagcacctgg ggagcaggat ggcgatgcta
     1621 tgcccttggg tagtgctgtg gatatcctgg ccacagatga tcccaacttt agccaggaag
     1681 atcagcagga cacccagatt tatgagaagc atgacaacct tctacatggg accaagaaga
     1741 aaaaggagaa gatggtgagt gcagcattca tgaagaagta catccatgtg gccaaaatca
     1801 tcaagcctgt cctgacacag gagtcggcca cctacattgc agaagagtat tcacgcctgc
     1861 gcagccagga tagcatgagc tcagacaccg ccaggacatc tccagttaca gcccgaacac
     1921 tggaaactct gattcgactg gccacagccc atgcgaaggc ccgcatgagc aagactgtgg
     1981 acctgcagga tgcagaggaa gctgtggagt tggtccagta tgcttacttt aagaaggttc
     2041 tggagaagga gaagaaacgt aagaagcgaa gtgaggatga atcagagaca gaagatgaag
     2101 aggagaaaag ccaagaggac caggagcaga agaggaagag aaggaagact cgccagccag
     2161 atgccaaaga tggggattca tacgacccct atgacttcag tgacacagag gaggaaatgc
     2221 ctcaagtaca cactccaaag acggcagact cacaggagac caaggaatcc cagaaagtgg
     2281 agttgagtga atccaggttg aaggcattca aggtggccct cttggatgtg ttccgggaag
     2341 ctcatgcgca gtcaatcggc atgaatcgcc tcacagaatc catcaaccgg gacagcgaag
     2401 agcccttctc ttcagttgag atccaggctg ctctgagcaa gatgcaggat gacaatcagg
     2461 tcatggtgtc tgagggcatc atcttcctca tctgaggagg cctcgtctct gaacttgggt
     2521 tgtgccgaga gagtttgttc tgtgtttccc accctctccc tgacccaagt ctttgcctct
     2581 actcccttaa cagtgttgaa ttcaactgaa ggcgaggaat gttggtgatg aagctgagtt
     2641 caggactcgg tggacccttt gggaatgggt catgaaagct gccatggggt gaggaaagag
     2701 gagacagtgg gagaggacaa tgactattgc atcttcattg caaaagcact ggctcatccg
     2761 ccctacttcc catcccacac aaacccaatt gtaaataaca tatgacttct gagtactttt
     2821 gggggcacaa ctgttttctg tttgctgttt ttttgttttg ttttttttct ccagagcact
     2881 ttggtctaga ctaggctttg ggtggttcca attggtggag agaagctctg aggcacgtca
     2941 tgcaggtcaa gaaagctttc tttgcagtag caccagttaa ggtgaatatg tattgtatca
     3001 caaaacaaac ccaatatcca gatgaatatc cgagatgttg aataaactta gccatttcgt
     3061 acacatgg
//
