LOCUS       NM_004526               3434 bp    mRNA    linear   PRI 06-AUG-2024
DEFINITION  Homo sapiens minichromosome maintenance complex component 2 (MCM2),
            transcript variant 1, mRNA.
ACCESSION   NM_004526
VERSION     NM_004526.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3434)
  AUTHORS   Zhong,L., Dong,Y. and Liu,S.
  TITLE     KNTC1 knockdown inhibits the proliferation and migration of
            osteosarcoma cells by MCM2
  JOURNAL   Mol Carcinog 63 (8), 1599-1610 (2024)
   PUBMED   38818892
  REMARK    GeneRIF: KNTC1 knockdown inhibits the proliferation and migration
            of osteosarcoma cells by MCM2.
REFERENCE   2  (bases 1 to 3434)
  AUTHORS   Kahm,Y.J., Kim,I.G. and Kim,R.K.
  TITLE     Regulation of cancer stem cells by CXCL1, a chemokine whose
            secretion is controlled by MCM2
  JOURNAL   BMC Cancer 24 (1), 319 (2024)
   PUBMED   38454443
  REMARK    GeneRIF: Regulation of cancer stem cells by CXCL1, a chemokine
            whose secretion is controlled by MCM2.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3434)
  AUTHORS   Liu,S., Liang,Z., Wang,Y., Ren,Y., Gu,Y., Qiao,Y., He,H., Li,Y.,
            Cheng,Y. and Liu,Y.
  TITLE     MCM2 is involved in subtyping and tamoxifen resistance of
            ERalpha-positive breast cancer by acting as the downstream factor
            of ERalpha
  JOURNAL   Biotechnol J 19 (2), e2300560 (2024)
   PUBMED   38403459
  REMARK    GeneRIF: MCM2 is involved in subtyping and tamoxifen resistance of
            ERalpha-positive breast cancer by acting as the downstream factor
            of ERalpha.
REFERENCE   4  (bases 1 to 3434)
  AUTHORS   Chen,K., Ning,X., Yan,X. and Song,L.
  TITLE     Circ_0104700 contributes to acute myeloid leukemia progression by
            enhancing MCM2 expression through targeting miR-665
  JOURNAL   Hematology 28 (1), 2227489 (2023)
   PUBMED   37358551
  REMARK    GeneRIF: Circ_0104700 contributes to acute myeloid leukemia
            progression by enhancing MCM2 expression through targeting miR-665.
REFERENCE   5  (bases 1 to 3434)
  AUTHORS   Chu,Y., Jin,X. and Guo,X.
  TITLE     MCM2 promotes the stemness of endometrial cancer cells via the
            Akt/beta-catenin pathway
  JOURNAL   Neoplasma 70 (5), 610-620 (2023)
   PUBMED   38053378
  REMARK    GeneRIF: MCM2 promotes the stemness of endometrial cancer cells via
            the Akt/beta-catenin pathway.
REFERENCE   6  (bases 1 to 3434)
  AUTHORS   Ishimi,Y., Ichinose,S., Omori,A., Sato,K. and Kimura,H.
  TITLE     Binding of human minichromosome maintenance proteins with histone
            H3
  JOURNAL   J Biol Chem 271 (39), 24115-24122 (1996)
   PUBMED   8798650
REFERENCE   7  (bases 1 to 3434)
  AUTHORS   Nakatsuru,S., Sudo,K. and Nakamura,Y.
  TITLE     Isolation and mapping of a human gene (MCM2) encoding a product
            homologous to yeast proteins involved in DNA replication
  JOURNAL   Cytogenet Cell Genet 68 (3-4), 226-230 (1995)
   PUBMED   7842741
REFERENCE   8  (bases 1 to 3434)
  AUTHORS   Todorov,I.T., Pepperkok,R., Philipova,R.N., Kearsey,S.E.,
            Ansorge,W. and Werner,D.
  TITLE     A human nuclear protein with sequence homology to a family of early
            S phase proteins is required for entry into S phase and for cell
            division
  JOURNAL   J Cell Sci 107 (Pt 1), 253-265 (1994)
   PUBMED   8175912
REFERENCE   9  (bases 1 to 3434)
  AUTHORS   Mincheva,A., Todorov,I., Werner,D., Fink,T.M. and Lichter,P.
  TITLE     The human gene for nuclear protein BM28 (CDCL1), a new member of
            the early S-phase family of proteins, maps to chromosome band 3q21
  JOURNAL   Cytogenet Cell Genet 65 (4), 276-277 (1994)
   PUBMED   8258304
REFERENCE   10 (bases 1 to 3434)
  AUTHORS   Todorov,I.T., Lavigne,J., Sakr,F., Kaneva,R., Foisy,S. and
            Bibor-Hardy,V.
  TITLE     Nuclear matrix protein mitotin messenger RNA is expressed at
            constant levels during the cell cycle
  JOURNAL   Biochem Biophys Res Commun 177 (1), 395-400 (1991)
   PUBMED   1710453
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DB100427.1 and BC007938.2.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_004526.3.
            
            Summary: The protein encoded by this gene is one of the highly
            conserved mini-chromosome maintenance proteins (MCM) that are
            involved in the initiation of eukaryotic genome replication. The
            hexameric protein complex formed by MCM proteins is a key component
            of the pre-replication complex (pre_RC) and may be involved in the
            formation of replication forks and in the recruitment of other DNA
            replication related proteins. This protein forms a complex with
            MCM4, 6, and 7, and has been shown to regulate the helicase
            activity of the complex. This protein is phosphorylated, and thus
            regulated by, protein kinases CDC2 and CDC7. Multiple alternatively
            spliced transcript variants have been found, but the full-length
            nature of some variants has not been defined. [provided by RefSeq,
            Oct 2012].
            
            Transcript Variant: This variant (1) encodes a functional protein.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC014272.2, SRR1803617.151518.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000265056.12/ ENSP00000265056.7
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-38                DB100427.1         55-92
            39-3434             BC007938.2         1-3396
FEATURES             Location/Qualifiers
     source          1..3434
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="3"
                     /map="3q21.3"
     gene            1..3434
                     /gene="MCM2"
                     /gene_synonym="BM28; CCNL1; cdc19; CDCL1; D3S3194; DFNA70;
                     MITOTIN"
                     /note="minichromosome maintenance complex component 2"
                     /db_xref="GeneID:4171"
                     /db_xref="HGNC:HGNC:6944"
                     /db_xref="MIM:116945"
     exon            1..62
                     /gene="MCM2"
                     /gene_synonym="BM28; CCNL1; cdc19; CDCL1; D3S3194; DFNA70;
                     MITOTIN"
                     /inference="alignment:Splign:2.1.0"
     CDS             57..2771
                     /gene="MCM2"
                     /gene_synonym="BM28; CCNL1; cdc19; CDCL1; D3S3194; DFNA70;
                     MITOTIN"
                     /EC_number="3.6.4.12"
                     /note="cyclin-like 1; minichromosome maintenance deficient
                     2 (mitotin); cell devision cycle-like 1; nuclear protein
                     BM28; minichromosome maintenance protein 2 homolog"
                     /codon_start=1
                     /product="DNA replication licensing factor MCM2"
                     /protein_id="NP_004517.2"
                     /db_xref="CCDS:CCDS3043.1"
                     /db_xref="GeneID:4171"
                     /db_xref="HGNC:HGNC:6944"
                     /db_xref="MIM:116945"
                     /translation="MAESSESFTMASSPAQRRRGNDPLTSSPGRSSRRTDALTSSPGR
                     DLPPFEDESEGLLGTEGPLEEEEDGEELIGDGMERDYRAIPELDAYEAEGLALDDEDV
                     EELTASQREAAERAMRQRDREAGRGLGRMRRGLLYDSDEEDEERPARKRRQVERATED
                     GEEDEEMIESIENLEDLKGHSVREWVSMAGPRLEIHHRFKNFLRTHVDSHGHNVFKER
                     ISDMCKENRESLVVNYEDLAAREHVLAYFLPEAPAELLQIFDEAALEVVLAMYPKYDR
                     ITNHIHVRISHLPLVEELRSLRQLHLNQLIRTSGVVTSCTGVLPQLSMVKYNCNKCNF
                     VLGPFCQSQNQEVKPGSCPECQSAGPFEVNMEETIYQNYQRIRIQESPGKVAAGRLPR
                     SKDAILLADLVDSCKPGDEIELTGIYHNNYDGSLNTANGFPVFATVILANHVAKKDNK
                     VAVGELTDEDVKMITSLSKDQQIGEKIFASIAPSIYGHEDIKRGLALALFGGEPKNPG
                     GKHKVRGDINVLLCGDPGTAKSQFLKYIEKVSSRAIFTTGQGASAVGLTAYVQRHPVS
                     REWTLEAGALVLADRGVCLIDEFDKMNDQDRTSIHEAMEQQSISISKAGIVTSLQARC
                     TVIAAANPIGGRYDPSLTFSENVDLTEPIISRFDILCVVRDTVDPVQDEMLARFVVGS
                     HVRHHPSNKEEEGLANGSAAEPAMPNTYGVEPLPQEVLKKYIIYAKERVHPKLNQMDQ
                     DKVAKMYSDLRKESMATGSIPITVRHIESMIRMAEAHARIHLRDYVIEDDVNMAIRVM
                     LESFIDTQKFSVMRSMRKTFARYLSFRRDNNELLLFILKQLVAEQVTYQRNRFGAQQD
                     TIEVPEKDLVDKARQINIHNLSAFYDSELFRMNKFSHDLKRKMILQQF"
     exon            63..292
                     /gene="MCM2"
                     /gene_synonym="BM28; CCNL1; cdc19; CDCL1; D3S3194; DFNA70;
                     MITOTIN"
                     /inference="alignment:Splign:2.1.0"
     exon            293..468
                     /gene="MCM2"
                     /gene_synonym="BM28; CCNL1; cdc19; CDCL1; D3S3194; DFNA70;
                     MITOTIN"
                     /inference="alignment:Splign:2.1.0"
     exon            469..729
                     /gene="MCM2"
                     /gene_synonym="BM28; CCNL1; cdc19; CDCL1; D3S3194; DFNA70;
                     MITOTIN"
                     /inference="alignment:Splign:2.1.0"
     exon            730..949
                     /gene="MCM2"
                     /gene_synonym="BM28; CCNL1; cdc19; CDCL1; D3S3194; DFNA70;
                     MITOTIN"
                     /inference="alignment:Splign:2.1.0"
     exon            950..1157
                     /gene="MCM2"
                     /gene_synonym="BM28; CCNL1; cdc19; CDCL1; D3S3194; DFNA70;
                     MITOTIN"
                     /inference="alignment:Splign:2.1.0"
     exon            1158..1292
                     /gene="MCM2"
                     /gene_synonym="BM28; CCNL1; cdc19; CDCL1; D3S3194; DFNA70;
                     MITOTIN"
                     /inference="alignment:Splign:2.1.0"
     exon            1293..1484
                     /gene="MCM2"
                     /gene_synonym="BM28; CCNL1; cdc19; CDCL1; D3S3194; DFNA70;
                     MITOTIN"
                     /inference="alignment:Splign:2.1.0"
     exon            1485..1578
                     /gene="MCM2"
                     /gene_synonym="BM28; CCNL1; cdc19; CDCL1; D3S3194; DFNA70;
                     MITOTIN"
                     /inference="alignment:Splign:2.1.0"
     exon            1579..1829
                     /gene="MCM2"
                     /gene_synonym="BM28; CCNL1; cdc19; CDCL1; D3S3194; DFNA70;
                     MITOTIN"
                     /inference="alignment:Splign:2.1.0"
     exon            1830..1956
                     /gene="MCM2"
                     /gene_synonym="BM28; CCNL1; cdc19; CDCL1; D3S3194; DFNA70;
                     MITOTIN"
                     /inference="alignment:Splign:2.1.0"
     exon            1957..2069
                     /gene="MCM2"
                     /gene_synonym="BM28; CCNL1; cdc19; CDCL1; D3S3194; DFNA70;
                     MITOTIN"
                     /inference="alignment:Splign:2.1.0"
     exon            2070..2321
                     /gene="MCM2"
                     /gene_synonym="BM28; CCNL1; cdc19; CDCL1; D3S3194; DFNA70;
                     MITOTIN"
                     /inference="alignment:Splign:2.1.0"
     exon            2322..2504
                     /gene="MCM2"
                     /gene_synonym="BM28; CCNL1; cdc19; CDCL1; D3S3194; DFNA70;
                     MITOTIN"
                     /inference="alignment:Splign:2.1.0"
     exon            2505..2660
                     /gene="MCM2"
                     /gene_synonym="BM28; CCNL1; cdc19; CDCL1; D3S3194; DFNA70;
                     MITOTIN"
                     /inference="alignment:Splign:2.1.0"
     exon            2661..3434
                     /gene="MCM2"
                     /gene_synonym="BM28; CCNL1; cdc19; CDCL1; D3S3194; DFNA70;
                     MITOTIN"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3412..3417
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MCM2"
                     /gene_synonym="BM28; CCNL1; cdc19; CDCL1; D3S3194; DFNA70;
                     MITOTIN"
                     /note="hexamer: AATAAA"
     polyA_site      3434
                     /gene="MCM2"
                     /gene_synonym="BM28; CCNL1; cdc19; CDCL1; D3S3194; DFNA70;
                     MITOTIN"
                     /note="major polyA site"
ORIGIN      
        1 cttttcgcgc gaaacctggt tgttgctgta gtggcggaga ggatcgtggt actgctatgg
       61 cggaatcatc ggaatccttc accatggcat ccagcccggc ccagcgtcgg cgaggcaatg
      121 atcctctcac ctccagccct ggccgaagct cccggcgtac tgatgccctc acctccagcc
      181 ctggccgtga ccttccacca tttgaggatg agtccgaggg gctcctaggc acagaggggc
      241 ccctggagga agaagaggat ggagaggagc tcattggaga tggcatggaa agggactacc
      301 gcgccatccc agagctggac gcctatgagg ccgagggact ggctctggat gatgaggacg
      361 tagaggagct gacggccagt cagagggagg cagcagagcg ggccatgcgg cagcgtgacc
      421 gggaggctgg ccggggcctg ggccgcatgc gccgtgggct cctgtatgac agcgatgagg
      481 aggacgagga gcgccctgcc cgcaagcgcc gccaggtgga gcgggccacg gaggacggcg
      541 aggaggacga ggagatgatc gagagcatcg agaacctgga ggatctcaaa ggccactctg
      601 tgcgcgagtg ggtgagcatg gcgggccccc ggctggagat ccaccaccgc ttcaagaact
      661 tcctgcgcac tcacgtcgac agccacggcc acaacgtctt caaggagcgc atcagcgaca
      721 tgtgcaaaga gaaccgtgag agcctggtgg tgaactatga ggacttggca gccagggagc
      781 acgtgctggc ctacttcctg cctgaggcac cggcggagct gctgcagatc tttgatgagg
      841 ctgccctgga ggtggtactg gccatgtacc ccaagtacga ccgcatcacc aaccacatcc
      901 atgtccgcat ctcccacctg cctctggtgg aggagctgcg ctcgctgagg cagctgcatc
      961 tgaaccagct gatccgcacc agtggggtgg tgaccagctg cactggcgtc ctgccccagc
     1021 tcagcatggt caagtacaac tgcaacaagt gcaatttcgt cctgggtcct ttctgccagt
     1081 cccagaacca ggaggtgaaa ccaggctcct gtcctgagtg ccagtcggcc ggcccctttg
     1141 aggtcaacat ggaggagacc atctatcaga actaccagcg tatccgaatc caggagagtc
     1201 caggcaaagt ggcggctggc cggctgcccc gctccaagga cgccattctc ctcgcagatc
     1261 tggtggacag ctgcaagcca ggagacgaga tagagctgac tggcatctat cacaacaact
     1321 atgatggctc cctcaacact gccaatggct tccctgtctt tgccactgtc atcctagcca
     1381 accacgtggc caagaaggac aacaaggttg ctgtagggga actgaccgat gaagatgtga
     1441 agatgatcac tagcctctcc aaggatcagc agatcggaga gaagatcttt gccagcattg
     1501 ctccttccat ctatggtcat gaagacatca agagaggcct ggctctggcc ctgttcggag
     1561 gggagcccaa aaacccaggt ggcaagcaca aggtacgtgg tgatatcaac gtgctcttgt
     1621 gcggagaccc tggcacagcg aagtcgcagt ttctcaagta tattgagaaa gtgtccagcc
     1681 gagccatctt caccactggc cagggggcgt cggctgtggg cctcacggcg tatgtccagc
     1741 ggcaccctgt cagcagggag tggaccttgg aggctggggc cctggttctg gctgaccgag
     1801 gagtgtgtct cattgatgaa tttgacaaga tgaatgacca ggacagaacc agcatccatg
     1861 aggccatgga gcaacagagc atctccatct cgaaggctgg catcgtcacc tccctgcagg
     1921 ctcgctgcac ggtcattgct gccgccaacc ccataggagg gcgctacgac ccctcgctga
     1981 ctttctctga gaacgtggac ctcacagagc ccatcatctc acgctttgac atcctgtgtg
     2041 tggtgaggga caccgtggac ccagtccagg acgagatgct ggcccgcttc gtggtgggca
     2101 gccacgtcag acaccacccc agcaacaagg aggaggaggg gctggccaat ggcagcgctg
     2161 ctgagcccgc catgcccaac acgtatggcg tggagcccct gccccaggag gtcctgaaga
     2221 agtacatcat ctacgccaag gagagggtcc acccgaagct caaccagatg gaccaggaca
     2281 aggtggccaa gatgtacagt gacctgagga aagaatctat ggcgacaggc agcatcccca
     2341 ttacggtgcg gcacatcgag tccatgatcc gcatggcgga ggcccacgcg cgcatccatc
     2401 tgcgggacta tgtgatcgaa gacgacgtca acatggccat ccgcgtgatg ctggagagct
     2461 tcatagacac acagaagttc agcgtcatgc gcagcatgcg caagactttt gcccgctacc
     2521 tttcattccg gcgtgacaac aatgagctgt tgctcttcat actgaagcag ttagtggcag
     2581 agcaggtgac atatcagcgc aaccgctttg gggcccagca ggacactatt gaggtccctg
     2641 agaaggactt ggtggataag gctcgtcaga tcaacatcca caacctctct gcattttatg
     2701 acagtgagct cttcaggatg aacaagttca gccacgacct gaaaaggaaa atgatcctgc
     2761 agcagttctg aggccctatg ccatccataa ggattccttg ggattctggt ttggggtggt
     2821 cagtgccctc tgtgctttat ggacacaaaa ccagagcact tgatgaactc ggggtactag
     2881 ggtcagggct tatagcagga tgtctggctg cacctggcat gactgtttgt ttctccaagc
     2941 ctgctttgtg cttctcacct ttgggtggga tgccttgcca gtgtgtctta cttggttgct
     3001 gaacatcttg ccacctccga gtgctttgtc tccactcagt accttggatc agagctgctg
     3061 agttcaggat gcctgcgtgt ggtttaggtg ttagccttct tacatggatg tcaggagagc
     3121 tgctgccctc ttggcgtgag ttgcgtattc aggctgcttt tgctgccttt ggccagagag
     3181 ctggttgaag atgtttgtaa tcgttttcag tctcctgcag gtttctgtgc ccctgtggtg
     3241 gaagagggca cgacagtgcc agcgcagcgt tctgggctcc tcagtcgcag gggtgggatg
     3301 tgagtcatgc ggattatcca ctcgccacag ttatcagctg ccattgctcc ctgtctgttt
     3361 ccccactctc ttatttgtgc attcggtttg gtttctgtag ttttaatttt taataaagtt
     3421 gaataaaata taaa
//
