LOCUS       NM_018518               4549 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens minichromosome maintenance 10 replication initiation
            factor (MCM10), transcript variant 2, mRNA.
ACCESSION   NM_018518
VERSION     NM_018518.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4549)
  AUTHORS   Schmit,M.M., Baxley,R.M., Wang,L., Hinderlie,P., Kaufman,M.,
            Simon,E., Raju,A., Miller,J.S. and Bielinsky,A.K.
  TITLE     A critical threshold of MCM10 is required to maintain genome
            stability during differentiation of induced pluripotent stem cells
            into natural killer cells
  JOURNAL   Open Biol 14 (1), 230407 (2024)
   PUBMED   38262603
  REMARK    GeneRIF: A critical threshold of MCM10 is required to maintain
            genome stability during differentiation of induced pluripotent stem
            cells into natural killer cells.
REFERENCE   2  (bases 1 to 4549)
  AUTHORS   Chen,D., Zhong,N., Guo,Z., Ji,Q., Dong,Z., Zheng,J., Ma,Y.,
            Zhang,J., He,Y. and Song,T.
  TITLE     MCM10, a potential diagnostic, immunological, and prognostic
            biomarker in pan-cancer
  JOURNAL   Sci Rep 13 (1), 17701 (2023)
   PUBMED   37848534
  REMARK    GeneRIF: MCM10, a potential diagnostic, immunological, and
            prognostic biomarker in pan-cancer.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 4549)
  AUTHORS   Caballero,M., Ge,T., Rebelo,A.R., Seo,S., Kim,S., Brooks,K.,
            Zuccaro,M., Kanagaraj,R., Vershkov,D., Kim,D., Smogorzewska,A.,
            Smolka,M., Benvenisty,N., West,S.C., Egli,D., Mace,E.M. and
            Koren,A.
  TITLE     Comprehensive analysis of DNA replication timing across 184 cell
            lines suggests a role for MCM10 in replication timing regulation
  JOURNAL   Hum Mol Genet 31 (17), 2899-2917 (2022)
   PUBMED   35394024
  REMARK    GeneRIF: Comprehensive analysis of DNA replication timing across
            184 cell lines suggests a role for MCM10 in replication timing
            regulation.
REFERENCE   4  (bases 1 to 4549)
  AUTHORS   Mughal,M.J., Chan,K.I., Mahadevappa,R., Wong,S.W., Wai,K.C. and
            Kwok,H.F.
  TITLE     Over-Activation of Minichromosome Maintenance Protein 10 Promotes
            Genomic Instability in Early Stages of Breast Cancer
  JOURNAL   Int J Biol Sci 18 (9), 3827-3844 (2022)
   PUBMED   35813483
  REMARK    GeneRIF: Over-Activation of Minichromosome Maintenance Protein 10
            Promotes Genomic Instability in Early Stages of Breast Cancer.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 4549)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   6  (bases 1 to 4549)
  AUTHORS   Zou,L. and Elledge,S.J.
  TITLE     Sensing DNA damage through ATRIP recognition of RPA-ssDNA complexes
  JOURNAL   Science 300 (5625), 1542-1548 (2003)
   PUBMED   12791985
REFERENCE   7  (bases 1 to 4549)
  AUTHORS   Wohlschlegel,J.A., Dhar,S.K., Prokhorova,T.A., Dutta,A. and
            Walter,J.C.
  TITLE     Xenopus Mcm10 binds to origins of DNA replication after Mcm2-7 and
            stimulates origin binding of Cdc45
  JOURNAL   Mol Cell 9 (2), 233-240 (2002)
   PUBMED   11864598
  REMARK    GeneRIF: Functional studies of the Xenopus homolog
REFERENCE   8  (bases 1 to 4549)
  AUTHORS   Bell,S.P. and Dutta,A.
  TITLE     DNA replication in eukaryotic cells
  JOURNAL   Annu Rev Biochem 71, 333-374 (2002)
   PUBMED   12045100
  REMARK    Review article
REFERENCE   9  (bases 1 to 4549)
  AUTHORS   Izumi,M., Yatagai,F. and Hanaoka,F.
  TITLE     Cell cycle-dependent proteolysis and phosphorylation of human Mcm10
  JOURNAL   J Biol Chem 276 (51), 48526-48531 (2001)
   PUBMED   11602595
REFERENCE   10 (bases 1 to 4549)
  AUTHORS   Izumi,M., Yanagi,K., Mizuno,T., Yokoi,M., Kawasaki,Y., Moon,K.Y.,
            Hurwitz,J., Yatagai,F. and Hanaoka,F.
  TITLE     The human homolog of Saccharomyces cerevisiae Mcm10 interacts with
            replication factors and dissociates from nuclease-resistant nuclear
            structures in G(2) phase
  JOURNAL   Nucleic Acids Res 28 (23), 4769-4777 (2000)
   PUBMED   11095689
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            DA024424.1, AB042719.1, AL136840.1 and AL138764.21.
            
            On Jun 2, 2019 this sequence version replaced NM_018518.4.
            
            Summary: The protein encoded by this gene is one of the highly
            conserved mini-chromosome maintenance proteins (MCM) that are
            involved in the initiation of eukaryotic genome replication. The
            hexameric protein complex formed by MCM proteins is a key component
            of the pre-replication complex (pre-RC) and it may be involved in
            the formation of replication forks and in the recruitment of other
            DNA replication related proteins. This protein can interact with
            MCM2 and MCM6, as well as with the origin recognition protein ORC2.
            It is regulated by proteolysis and phosphorylation in a cell
            cycle-dependent manner. Studies of a similar protein in Xenopus
            suggest that the chromatin binding of this protein at the onset of
            DNA replication is after pre-RC assembly and before origin
            unwinding. Alternatively spliced transcript variants encoding
            distinct isoforms have been identified. [provided by RefSeq, Jul
            2008].
            
            Transcript Variant: This variant (2) encodes the predominant
            isoform (2).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AB042719.1, BC143490.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000378714.8/ ENSP00000367986.3
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-29                DA024424.1         5-33
            30-3750             AB042719.1         1-3721
            3751-4543           AL136840.1         3815-4607
            4544-4549           AL138764.21        40430-40435
FEATURES             Location/Qualifiers
     source          1..4549
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="10"
                     /map="10p13"
     gene            1..4549
                     /gene="MCM10"
                     /gene_synonym="CNA43; DNA43; IMD80; PRO2249"
                     /note="minichromosome maintenance 10 replication
                     initiation factor"
                     /db_xref="GeneID:55388"
                     /db_xref="HGNC:HGNC:18043"
                     /db_xref="MIM:609357"
     exon            1..49
                     /gene="MCM10"
                     /gene_synonym="CNA43; DNA43; IMD80; PRO2249"
                     /inference="alignment:Splign:2.1.0"
     exon            50..131
                     /gene="MCM10"
                     /gene_synonym="CNA43; DNA43; IMD80; PRO2249"
                     /inference="alignment:Splign:2.1.0"
     CDS             125..2749
                     /gene="MCM10"
                     /gene_synonym="CNA43; DNA43; IMD80; PRO2249"
                     /note="isoform 2 is encoded by transcript variant 2;
                     homolog of yeast MCM10; protein MCM10 homolog; hsMCM10;
                     MCM10 minichromosome maintenance deficient 10;
                     minichromosome maintenance complex component 10"
                     /codon_start=1
                     /product="protein MCM10 homolog isoform 2"
                     /protein_id="NP_060988.3"
                     /db_xref="CCDS:CCDS7095.1"
                     /db_xref="GeneID:55388"
                     /db_xref="HGNC:HGNC:18043"
                     /db_xref="MIM:609357"
                     /translation="MDEEEDNLSLLTALLEENESALDCNSEENNFLTRENGEPDAFDE
                     LFDADGDGESYTEEADDGETGETRDEKENLATLFGDMEDLTDEEEVPASQSTENRVLP
                     APAPRREKTNEELQEELRNLQEQMKALQEQLKVTTIKQTASPARLQKSPEKSPRPPLK
                     ERRVQRIQESTCFSAELDVPALPRTKRVARTPKASPPDPKSSSSRMTSAPSQPLQTIS
                     RNKPSGITRGQIVGTPGSSGETTQPICVEAFSGLRLRRPRVSSTEMNKKMTGRKLIRL
                     SQIKEKMAREKLEEIDWVTFGVILKKVTPQSVNSGKTFSIWKLNDLRDLTQCVSLFLF
                     GEVHKALWKTEQGTVVGILNANPMKPKDGSEEVCLSIDHPQKVLIMGEALDLGTCKAK
                     KKNGEPCTQTVNLRDCEYCQYHVQAQYKKLSAKRADLQSTFSGGRIPKKFARRGTSLK
                     ERLCQDGFYYGGVSSASYAASIAAAVAPKKKIQTTLSNLVVKGTNLIIQETRQKLGIP
                     QKSLSCSEEFKELMDLPTCGARNLKQHLAKATASGIMGSPKPAIKSISASALLKQQKQ
                     RMLEMRRRKSEEIQKRFLQSSSEVESPAVPSSSRQPPAQPPRTGSEFPRLEGAPATMT
                     PKLGRGVLEGDDVLFYDESPPPRPKLSALAEAKKLAAITKLRAKGQVLTKTNPNSIKK
                     KQKDPQDILEVKERVEKNTMFSSQAEDELEPARKKRREQLAYLESEEFQKILKAKSKH
                     TGILKEAEAEMQERYFEPLVKKEQMEEKMRNIREVKCRVVTCKTCAYTHFKLLETCVS
                     EQHEYHWHDGVKRFFKCPCGNRSISLDRLPNKHCSNCGLYKWERDGMLKEKTGPKIGG
                     ETLLPRGEEHAKFLNSLK"
     exon            132..473
                     /gene="MCM10"
                     /gene_synonym="CNA43; DNA43; IMD80; PRO2249"
                     /inference="alignment:Splign:2.1.0"
     exon            474..578
                     /gene="MCM10"
                     /gene_synonym="CNA43; DNA43; IMD80; PRO2249"
                     /inference="alignment:Splign:2.1.0"
     exon            579..716
                     /gene="MCM10"
                     /gene_synonym="CNA43; DNA43; IMD80; PRO2249"
                     /inference="alignment:Splign:2.1.0"
     exon            717..888
                     /gene="MCM10"
                     /gene_synonym="CNA43; DNA43; IMD80; PRO2249"
                     /inference="alignment:Splign:2.1.0"
     exon            889..1054
                     /gene="MCM10"
                     /gene_synonym="CNA43; DNA43; IMD80; PRO2249"
                     /inference="alignment:Splign:2.1.0"
     exon            1055..1222
                     /gene="MCM10"
                     /gene_synonym="CNA43; DNA43; IMD80; PRO2249"
                     /inference="alignment:Splign:2.1.0"
     exon            1223..1339
                     /gene="MCM10"
                     /gene_synonym="CNA43; DNA43; IMD80; PRO2249"
                     /inference="alignment:Splign:2.1.0"
     exon            1340..1539
                     /gene="MCM10"
                     /gene_synonym="CNA43; DNA43; IMD80; PRO2249"
                     /inference="alignment:Splign:2.1.0"
     exon            1540..1640
                     /gene="MCM10"
                     /gene_synonym="CNA43; DNA43; IMD80; PRO2249"
                     /inference="alignment:Splign:2.1.0"
     exon            1641..1751
                     /gene="MCM10"
                     /gene_synonym="CNA43; DNA43; IMD80; PRO2249"
                     /inference="alignment:Splign:2.1.0"
     exon            1752..1869
                     /gene="MCM10"
                     /gene_synonym="CNA43; DNA43; IMD80; PRO2249"
                     /inference="alignment:Splign:2.1.0"
     exon            1870..2098
                     /gene="MCM10"
                     /gene_synonym="CNA43; DNA43; IMD80; PRO2249"
                     /inference="alignment:Splign:2.1.0"
     exon            2099..2243
                     /gene="MCM10"
                     /gene_synonym="CNA43; DNA43; IMD80; PRO2249"
                     /inference="alignment:Splign:2.1.0"
     exon            2244..2362
                     /gene="MCM10"
                     /gene_synonym="CNA43; DNA43; IMD80; PRO2249"
                     /inference="alignment:Splign:2.1.0"
     exon            2363..2476
                     /gene="MCM10"
                     /gene_synonym="CNA43; DNA43; IMD80; PRO2249"
                     /inference="alignment:Splign:2.1.0"
     exon            2477..2622
                     /gene="MCM10"
                     /gene_synonym="CNA43; DNA43; IMD80; PRO2249"
                     /inference="alignment:Splign:2.1.0"
     exon            2623..2665
                     /gene="MCM10"
                     /gene_synonym="CNA43; DNA43; IMD80; PRO2249"
                     /inference="alignment:Splign:2.1.0"
     exon            2666..4549
                     /gene="MCM10"
                     /gene_synonym="CNA43; DNA43; IMD80; PRO2249"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3314..3319
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MCM10"
                     /gene_synonym="CNA43; DNA43; IMD80; PRO2249"
                     /note="hexamer: ATTAAA"
     polyA_site      3339
                     /gene="MCM10"
                     /gene_synonym="CNA43; DNA43; IMD80; PRO2249"
                     /note="major polyA site"
     regulatory      4526..4531
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MCM10"
                     /gene_synonym="CNA43; DNA43; IMD80; PRO2249"
                     /note="hexamer: AATAAA"
     polyA_site      4549
                     /gene="MCM10"
                     /gene_synonym="CNA43; DNA43; IMD80; PRO2249"
                     /note="major polyA site"
ORIGIN      
        1 ggaattttgg cgggttcagc tgtgaacgaa gaaggcgtcc cggcatcggc caagattcta
       61 cattgctcat ctgggcatct gagcctcctt cgaagtttcc tgtcacaact gtcctcttga
      121 cagcatggat gaggaggaag acaatctgtc tctgctgacc gcactgctgg aagaaaatga
      181 gtcagccttg gattgtaatt cagaagaaaa taacttcttg acgcgggaaa atggcgagcc
      241 cgacgcattt gatgagctct ttgatgccga cggcgacggt gaatcttata cagaagaggc
      301 tgatgatgga gaaacaggag agacaagaga cgaaaaggaa aatctggcca ctctctttgg
      361 agatatggag gacttaacag atgaagaaga agttcccgca tcacagtcaa ctgaaaatag
      421 ggtcctccct gctcctgccc ccaggcgaga gaaaacgaat gaagagttgc aagaggaatt
      481 aaggaatttg caagagcaaa tgaaggcctt acaagagcag ctaaaagtaa caacaattaa
      541 acagacagca agcccagccc gtctgcaaaa atcccctgag aagtctcccc ggccacctct
      601 taaggagagg agagttcaga gaattcagga gtcaacatgc ttttctgcgg agcttgatgt
      661 ccctgcgcta ccaagaacca agagggtggc tcgaacacca aaggcttcac ctccagatcc
      721 caaaagctca tcttcaagga tgacaagtgc accctcccaa cccctacaga cgatttctcg
      781 gaacaaacct agtgggataa ctagaggtca aattgtgggg accccaggaa gttctgggga
      841 aacgactcaa cccatctgtg tggaagcctt ctctggtctg cggctcaggc ggcctcgagt
      901 atcctccaca gaaatgaaca agaaaatgac cggccgaaaa ctgatcagac tgtctcagat
      961 caaggaaaag atggccagag agaagctgga agaaatagat tgggtgacat ttggggttat
     1021 attgaagaag gttacgccac agagtgtgaa tagtggaaaa accttcagca tatggaaact
     1081 gaatgatctt cgtgacctga cacaatgtgt gtccttgttc ttatttggag aagttcacaa
     1141 agcgctctgg aagacggagc aggggactgt cgtagggatc ctcaatgcca accccatgaa
     1201 gcccaaggat ggttcagagg aggtgtgttt atctatcgat catcctcaga aggtcttaat
     1261 tatgggtgaa gctcttgacc tgggaacctg taaagccaag aagaagaatg gagagccgtg
     1321 cacgcagact gtgaatttgc gtgactgtga gtactgtcag taccatgtcc aggctcagta
     1381 caagaagctc agcgcaaagc gtgcggatct gcagtccacc ttctctggag gacgaattcc
     1441 aaagaagttt gcccgcagag gcaccagcct caaagaacgg ctgtgccaag atggctttta
     1501 ctacggaggg gtttcttctg cctcgtatgc agcttcaatt gcagcagctg tggctcctaa
     1561 gaagaagatt caaaccactc tgagtaatct ggttgttaag ggcacaaact tgatcatcca
     1621 ggaaacacgg caaaaactcg gaatacccca gaagagcctg tcttgctctg aggagttcaa
     1681 ggaactgatg gacctgccga cgtgtggagc caggaactta aaacaacatt tagccaaagc
     1741 cacagcttca gggattatgg ggagcccaaa accagccatc aagtccatct cggcctcagc
     1801 actcttgaag caacagaagc agcggatgtt ggagatgagg agaaggaaat cagaagaaat
     1861 acagaagcga tttctgcaga gctcaagtga agttgagagc ccagctgtgc catcttcatc
     1921 aagacagccc cctgctcagc ctccacggac aggatccgag ttccccaggc tggagggagc
     1981 cccggccaca atgacgccca agctggggcg aggtgtcttg gaaggagatg atgttctctt
     2041 ttatgatgag tcaccaccac caagaccaaa actgagtgct ttagcagaag ccaaaaagtt
     2101 agctgctatc accaaattaa gggcaaaagg ccaggttctt acaaaaacaa acccaaacag
     2161 cattaagaag aaacaaaagg accctcagga catcctggag gtgaaggaac gtgtagaaaa
     2221 aaacaccatg ttttcttctc aagctgagga tgaattggag cctgccagga aaaaaaggag
     2281 agaacaactt gcctatctgg aatctgagga atttcagaaa atcctaaaag caaaatcaaa
     2341 acacacaggc atcctgaaag aggccgaggc tgagatgcag gagcgctact ttgagccact
     2401 ggtgaaaaaa gaacaaatgg aagaaaagat gagaaacatc agagaagtga agtgccgtgt
     2461 cgtgacatgc aagacgtgcg cctataccca cttcaagctg ctggagacct gcgtcagtga
     2521 gcagcatgaa taccactggc atgatggtgt gaagaggttt ttcaaatgtc cctgtggaaa
     2581 cagaagcatc tccttggaca gactcccgaa caagcactgc agtaactgtg gcctctacaa
     2641 atgggaacgg gacggaatgc taaaggaaaa gactggtcca aagataggag gagaaactct
     2701 gttaccaaga ggagaagaac atgctaaatt tctgaacagc cttaaataac ccgaacttca
     2761 gacattttcc cacagacttc ctggcctcct gtgactctgg aaagcaaagg attggctgtg
     2821 tattgtccat tgattcctga ttgacgccgt caaaaacaaa tgcttgttaa gcccataagc
     2881 tttgcctgct tactttctgc cattgggttg gtttgatacc acatttaaca ttgacattta
     2941 agtggaaaac caagttatca ttgtcttttc taagctcagt gtggatgatt gcattacttc
     3001 attcactgaa gtttttgccc aaaaattgga aggtaaacag agagctatgt ttctgtatct
     3061 tttggttata gagtgttcac ttctttatca taacaaaatt ctagtgttta tacgaacacc
     3121 cagaggcaaa agaatttggc ttaattctca ctccaggtaa gtagcttaac ttctgggctt
     3181 cagttttctc atctgtaaaa tcaggaagat tggactaagt gatcctgaaa tgtatttttt
     3241 agcactggat ttctacaaat aataaaactt tcccatctag ataatgatga tcacatagtc
     3301 ttgatgtacg gacattaaaa gccagatttc ttcattcaat tctgttatct ctgttttact
     3361 ctttgaaatt gatcaagcca ctgaatcact ttgcatttca gtttatatat atagagagaa
     3421 agaaggtgtc tgctcttaca ttattgtgga gccctgtgat agaaatatgt aaaatctcat
     3481 attatttttt ttttaatttt tttatttttt atgacagggt ctcactatgt caccctggct
     3541 ggagtgcagt agtgcgatcg cggcacactg cagccttggc ttccctgggc tcaagcagtc
     3601 ctcccacctc agtctcccaa atagctagga ctacaggcgt gcgtgaccaa gcccagctaa
     3661 tttttgcatt ttttgtagag atggggtttt gccatgttgc tcaggctggt ctcaaactcc
     3721 tgagcactag caatccaccc acctctgttt ccaaaaaaaa aaaaaaaatg aaaggtcaac
     3781 ccctatgcaa attaccacag caaaggtttc attcaggaga ttcttccatc tgggcaacct
     3841 ggttttccaa atatcatttg acctaagtga atgttgatac tagctaaaga ttgggtaaat
     3901 tggttgaatt attgtattga agcttgagct gtagctaaaa gtaatttagg tttcccctaa
     3961 gatgttatta tgttagggac ataacacttt tgggaggttg ttgtgggaga tggttgattt
     4021 aggttttcaa aagctagaaa taaaatttac atgccttaga tttcataaaa ttctgctcta
     4081 attgggtgga aggtgctgta tctaacttgt gttcctccta aggttatgtc ctaataacta
     4141 ttcttttagg agtatacttc tactttatag aaggttgctt ttctttttaa ttttttctaa
     4201 caaagaaaag aataaagtat ttattaataa gaaccagaaa gcacttgaaa ctgatgtttt
     4261 taatggctca tttagggtag atttatttat ctcattaact taaaacagct atgtgtatga
     4321 aataggtcac aacagaactt gaacaccagg ttggtgtctg agcaatccct ttcttatggg
     4381 aaaaacaatg ttcttgtttg aacagagggt atcattgcag tcagtattca cgtgtatatt
     4441 gttatataag ttgtataata tgcttgtaaa ggctgagggt gagctgtatc tggatgcctt
     4501 tttacaattt gattttaact tttaaaataa atttaaaaca taattgtca
//
