LOCUS       NM_015078               5326 bp    mRNA    linear   PRI 07-APR-2024
DEFINITION  Homo sapiens MCF.2 cell line derived transforming sequence-like 2
            (MCF2L2), mRNA.
ACCESSION   NM_015078
VERSION     NM_015078.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5326)
  AUTHORS   Zhang,D., Efendic,S., Brismar,K. and Gu,H.F.
  TITLE     Effects of MCF2L2, ADIPOQ and SOX2 genetic polymorphisms on the
            development of nephropathy in type 1 Diabetes Mellitus
  JOURNAL   BMC Med Genet 11, 116 (2010)
   PUBMED   20667095
  REMARK    GeneRIF: MCF2L2 Leu359Ile polymorphism is associated with decreased
            risk of diabetic nephropathy in female type 1 diabetes mellitus.
            GeneRIF: Observational study of gene-disease association and
            gene-gene interaction. (HuGE Navigator)
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5326)
  AUTHORS   Zheng,Q., Shi,Y., Yang,Z., Xu,X., Wang,L., Xue,F., Gu,H.F. and
            Chen,Z.J.
  TITLE     Family-based association study of the MCF2L2 gene and polycystic
            ovary syndrome
  JOURNAL   Gynecol Obstet Invest 68 (3), 171-173 (2009)
   PUBMED   19648752
  REMARK    GeneRIF: Results suggest that the genetic polymorphisms within
            MCF2L2 may confer an increased susceptibility to PCOS in the
            Chinese population. Data may provide a basis for further studies of
            the role of the MCF2L2 gene in the etiology of PCOS.
            GeneRIF: Observational study of gene-disease association. (HuGE
            Navigator)
REFERENCE   3  (bases 1 to 5326)
  AUTHORS   Takeuchi,F., Ochiai,Y., Serizawa,M., Yanai,K., Kuzuya,N., Kajio,H.,
            Honjo,S., Takeda,N., Kaburagi,Y., Yasuda,K., Shirasawa,S.,
            Sasazuki,T. and Kato,N.
  TITLE     Search for type 2 diabetes susceptibility genes on chromosomes 1q,
            3q and 12q
  JOURNAL   J Hum Genet 53 (4), 314-324 (2008)
   PUBMED   18259684
  REMARK    GeneRIF: Observational study of gene-disease association. (HuGE
            Navigator)
REFERENCE   4  (bases 1 to 5326)
  AUTHORS   Nakayama,M., Kikuno,R. and Ohara,O.
  TITLE     Protein-protein interactions between large proteins: two-hybrid
            screening using a functionally classified library composed of long
            cDNAs
  JOURNAL   Genome Res 12 (11), 1773-1784 (2002)
   PUBMED   12421765
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC092960.3, AC112647.6, AC069417.19 and AC104641.11.
            
            On Aug 13, 2020 this sequence version replaced NM_015078.3.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660805.230316.1,
                                           SRR1803611.96154.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000328913.8/ ENSP00000328118.3
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-718               AC092960.3         70303-71020
            719-802             AC092960.3         109143-109226
            803-917             AC092960.3         119511-119625
            918-1008            AC092960.3         157292-157382
            1009-1128           AC092960.3         160003-160122
            1129-1245           AC112647.6         24743-24859         c
            1246-1395           AC112647.6         19576-19725         c
            1396-1520           AC112647.6         13156-13280         c
            1521-1635           AC112647.6         12423-12537         c
            1636-1755           AC112647.6         11224-11343         c
            1756-1947           AC069417.19        297-488
            1948-2139           AC069417.19        3326-3517
            2140-2317           AC069417.19        5016-5193
            2318-2418           AC069417.19        11273-11373
            2419-2504           AC069417.19        23536-23621
            2505-2571           AC069417.19        69476-69542
            2572-2687           AC069417.19        70712-70827
            2688-2757           AC069417.19        72127-72196
            2758-2850           AC069417.19        76303-76395
            2851-2943           AC069417.19        77055-77147
            2944-3012           AC069417.19        80569-80637
            3013-3138           AC069417.19        84399-84524
            3139-3354           AC069417.19        92670-92885
            3355-3447           AC069417.19        94279-94371
            3448-3526           AC069417.19        94539-94617
            3527-3560           AC104641.11        181927-181960       c
            3561-3658           AC104641.11        179704-179801       c
            3659-3747           AC104641.11        166776-166864       c
            3748-3863           AC104641.11        166282-166397       c
            3864-5326           AC104641.11        164746-166208       c
FEATURES             Location/Qualifiers
     source          1..5326
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="3"
                     /map="3q27.1"
     gene            1..5326
                     /gene="MCF2L2"
                     /gene_synonym="ARHGEF22"
                     /note="MCF.2 cell line derived transforming sequence-like
                     2"
                     /db_xref="GeneID:23101"
                     /db_xref="HGNC:HGNC:30319"
                     /db_xref="MIM:619946"
     exon            1..718
                     /gene="MCF2L2"
                     /gene_synonym="ARHGEF22"
                     /inference="alignment:Splign:2.1.0"
     CDS             643..3987
                     /gene="MCF2L2"
                     /gene_synonym="ARHGEF22"
                     /note="MCF2-transforming sequence-like protein 2;
                     dbs-related Rho family guanine nucleotide exchange factor"
                     /codon_start=1
                     /product="probable guanine nucleotide exchange factor
                     MCF2L2"
                     /protein_id="NP_055893.4"
                     /db_xref="CCDS:CCDS3243.1"
                     /db_xref="GeneID:23101"
                     /db_xref="HGNC:HGNC:30319"
                     /db_xref="MIM:619946"
                     /translation="MLSCLKEEMPPQELTRRLATVITHVDEIMQQEVRPLMAVEIIEQ
                     LHRQFAILSGGRGEDGAPIITFPEFSGFKHIPDEDFLNVMTYLTSIPSVEAASIGFIV
                     VIDRRRDKWSSVKASLTRIAVAFPGNLQLIFILRPSRFIQRTFTDIGIKYYRNEFKTK
                     VPIIMVNSVSDLHGYIDKSQLTRELGGTLEYRHGQWVNHRTAIENFALTLKTTAQMLQ
                     TFGSCLATAELPRSMLSTEDLLMSHTRQRDKLQDELKLLGKQGTTLLSCIQEPATKCP
                     NSKLNLNQLENVTTMERLLVQLDETEKAFSHFWSEHHLKLNQCLQLQHFEHDFCKAKL
                     ALDNLLEEQAEFTGIGDSVMHVEQILKEHKKLEEKSQEPLEKAQLLALVGDQLIQSHH
                     YAADAIRPRCVELRHLCDDFINGNKKKWDILGKSLEFHRQLDKVSQWCEAGIYLLASQ
                     AVDKCQSREGVDIALNDIATFLGTVKEYPLLSPKEFYNEFELLLTLDAKAKAQKVLQR
                     LDDVQEIFHKRQVSLMKLAAKQTRPVQPVAPHPESSPKWVSSKTSQPSTSVPLARPLR
                     TSEEPYTETELNSRGKEDDETKFEVKSEEIFESHHERGNPELEQQARLGDLSPRRRII
                     RDLLETEEIYIKEIKSIIDGYITPMDFIWLKHLIPDVLQNNKDFLFGNIRELYEFHNR
                     TFLKELEKCAENPELLAHCFLKRKEDLQIYFKYHKNLPRARAIWQECQDCAYFGVCQR
                     QLDHNLPLFKYLKGPSQRLIKYQMLLKGLLDFESPEDMEIDPGELGGSAKDGPKRTKD
                     SAFSTELQQALAVIEDLIKSCELAVDLAAVTECPDDIGKLGKLLLHGPFSVWTIHKDR
                     YKMKDLIRFKPSQRQIYLFERGIVFCKIRMEPGDQGLSPHYSFKKTMKLMTLSIRQLG
                     RGSHRKFEIASRNGLEKYILQAASKEIRDCWFSEISKLLMEQQNNIKDQGNPQFEMST
                     SKGSGAGSGPWIKNMERATTSKEDPASSTGGIKGCSSREFSSMDTFEDCEGAEDMEKE
                     SSALSLAGLFQSDDSHETCSSKSAFLERGESSQGEKEERDEEETATRSTEEERAGAST
                     GRLAPAGATAGFQARALRPRTSAQES"
     exon            719..802
                     /gene="MCF2L2"
                     /gene_synonym="ARHGEF22"
                     /inference="alignment:Splign:2.1.0"
     exon            803..917
                     /gene="MCF2L2"
                     /gene_synonym="ARHGEF22"
                     /inference="alignment:Splign:2.1.0"
     exon            918..1008
                     /gene="MCF2L2"
                     /gene_synonym="ARHGEF22"
                     /inference="alignment:Splign:2.1.0"
     exon            1009..1128
                     /gene="MCF2L2"
                     /gene_synonym="ARHGEF22"
                     /inference="alignment:Splign:2.1.0"
     exon            1129..1245
                     /gene="MCF2L2"
                     /gene_synonym="ARHGEF22"
                     /inference="alignment:Splign:2.1.0"
     exon            1246..1395
                     /gene="MCF2L2"
                     /gene_synonym="ARHGEF22"
                     /inference="alignment:Splign:2.1.0"
     exon            1396..1520
                     /gene="MCF2L2"
                     /gene_synonym="ARHGEF22"
                     /inference="alignment:Splign:2.1.0"
     exon            1521..1635
                     /gene="MCF2L2"
                     /gene_synonym="ARHGEF22"
                     /inference="alignment:Splign:2.1.0"
     exon            1636..1755
                     /gene="MCF2L2"
                     /gene_synonym="ARHGEF22"
                     /inference="alignment:Splign:2.1.0"
     exon            1756..1947
                     /gene="MCF2L2"
                     /gene_synonym="ARHGEF22"
                     /inference="alignment:Splign:2.1.0"
     exon            1948..2139
                     /gene="MCF2L2"
                     /gene_synonym="ARHGEF22"
                     /inference="alignment:Splign:2.1.0"
     exon            2140..2317
                     /gene="MCF2L2"
                     /gene_synonym="ARHGEF22"
                     /inference="alignment:Splign:2.1.0"
     exon            2318..2418
                     /gene="MCF2L2"
                     /gene_synonym="ARHGEF22"
                     /inference="alignment:Splign:2.1.0"
     exon            2419..2504
                     /gene="MCF2L2"
                     /gene_synonym="ARHGEF22"
                     /inference="alignment:Splign:2.1.0"
     exon            2505..2571
                     /gene="MCF2L2"
                     /gene_synonym="ARHGEF22"
                     /inference="alignment:Splign:2.1.0"
     exon            2572..2687
                     /gene="MCF2L2"
                     /gene_synonym="ARHGEF22"
                     /inference="alignment:Splign:2.1.0"
     exon            2688..2757
                     /gene="MCF2L2"
                     /gene_synonym="ARHGEF22"
                     /inference="alignment:Splign:2.1.0"
     exon            2758..2850
                     /gene="MCF2L2"
                     /gene_synonym="ARHGEF22"
                     /inference="alignment:Splign:2.1.0"
     exon            2851..2943
                     /gene="MCF2L2"
                     /gene_synonym="ARHGEF22"
                     /inference="alignment:Splign:2.1.0"
     exon            2944..3012
                     /gene="MCF2L2"
                     /gene_synonym="ARHGEF22"
                     /inference="alignment:Splign:2.1.0"
     exon            3013..3138
                     /gene="MCF2L2"
                     /gene_synonym="ARHGEF22"
                     /inference="alignment:Splign:2.1.0"
     exon            3139..3354
                     /gene="MCF2L2"
                     /gene_synonym="ARHGEF22"
                     /inference="alignment:Splign:2.1.0"
     exon            3355..3447
                     /gene="MCF2L2"
                     /gene_synonym="ARHGEF22"
                     /inference="alignment:Splign:2.1.0"
     exon            3448..3526
                     /gene="MCF2L2"
                     /gene_synonym="ARHGEF22"
                     /inference="alignment:Splign:2.1.0"
     exon            3527..3560
                     /gene="MCF2L2"
                     /gene_synonym="ARHGEF22"
                     /inference="alignment:Splign:2.1.0"
     exon            3561..3658
                     /gene="MCF2L2"
                     /gene_synonym="ARHGEF22"
                     /inference="alignment:Splign:2.1.0"
     exon            3659..3747
                     /gene="MCF2L2"
                     /gene_synonym="ARHGEF22"
                     /inference="alignment:Splign:2.1.0"
     exon            3748..3863
                     /gene="MCF2L2"
                     /gene_synonym="ARHGEF22"
                     /inference="alignment:Splign:2.1.0"
     exon            3864..5326
                     /gene="MCF2L2"
                     /gene_synonym="ARHGEF22"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5300..5305
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MCF2L2"
                     /gene_synonym="ARHGEF22"
                     /note="hexamer: ATTAAA"
     polyA_site      5326
                     /gene="MCF2L2"
                     /gene_synonym="ARHGEF22"
                     /note="major polyA site"
ORIGIN      
        1 agtcactgtc tccccgcccc acggttccga gtaaggaccg gagctccact ccgggggtgg
       61 agcgcgggct ccccgcaggg aaaggtgcgc agcgttcccg accccaaaac gagcggcgct
      121 tgggagcgtg cgtgcctccc cgagagcccc gggcgccacc cgtgggctgg acgaggtggg
      181 cgctggggcg tggggccggc ggcgcagaga agccacggcc acctgccgcc tcccctgcac
      241 ctggcggagc atccctcact ctacagtccc tggaggcggc gccggaaacc tggcggccgg
      301 gaccgcgatt ctggaagccc cggagctcgc ccggccggag gagccggggc gcggttccgg
      361 cgagattggc gacttgcttc cttttgcaga acggccgctc ctctctcggt ccagctgcga
      421 cgctgggaaa gcgccagact tggtctgtca cgggagcccc cgcggggcgt ggcgagggag
      481 agctggccta ggctgcgagg gactctcgtt tcttgcctgg agagccagga agagggcgag
      541 ggcagagcat ccttgggcgg agatgccttt aaaaaatcat ccaccgcagc ggtagaaaca
      601 gttttgtttg gctttattta tacggaatgg tttttcagtg aaatgctgtc ttgcttaaaa
      661 gaagagatgc ctccccagga gctcacccgg cgactggcca cagtgatcac tcatgtcgat
      721 gaaattatgc agcaggaagt cagacccctg atggcggtgg agataataga acaacttcac
      781 agacaatttg ccattctttc aggaggccga ggggaggatg gcgcccccat catcacgttc
      841 ccagagtttt cggggttcaa acacatccca gatgaagact tcctgaatgt catgacctac
      901 ctgactagca tccccagtgt ggaggctgcc agcattggat tcattgttgt tatcgacaga
      961 cgaagagaca agtggagctc cgtaaaggca tccttgacac gaatagctgt ggcatttcca
     1021 ggaaacttac agctcatatt catccttcgt ccatctcgct ttatccagag gacattcact
     1081 gacattggca ttaaatacta tcgaaatgag tttaaaacga aagtgccgat catcatggta
     1141 aactctgtct ctgaccttca cggctacatc gacaaaagcc aactgacccg ggaattaggg
     1201 gggactttgg aatatcgcca cggtcagtgg gtaaatcacc gcactgccat cgaaaacttt
     1261 gccttgacct tgaagaccac tgcccagatg ctgcagacgt ttgggtcctg cctggccaca
     1321 gcagagctgc ccagaagcat gctatccacg gaagaccttc tcatgtccca cacaaggcag
     1381 cgggacaagc tgcaggatga gctgaaatta cttggaaagc aggggaccac attgctgtca
     1441 tgcatccaag aaccagcaac caaatgtccc aacagcaaac tcaatctcaa ccaacttgag
     1501 aatgtaacta ccatggaaag gttattagtt caactggatg aaacagaaaa agcctttagt
     1561 cacttttggt ctgagcatca tctgaagctt aaccagtgcc tacaactaca gcattttgag
     1621 cacgattttt gtaaggctaa gcttgccctg gataatttgc tggaagagca agcagagttt
     1681 acaggcattg gagacagcgt gatgcacgtg gagcagattc ttaaggaaca caaaaaactg
     1741 gaggaaaaaa gccaggagcc cctggaaaag gcccagctgc tggcactggt tggggaccag
     1801 ctcatccaaa gccaccatta tgcagcagat gccatcaggc cccggtgtgt ggagctcagg
     1861 cacctctgtg acgatttcat caatggaaac aagaaaaaat gggacatttt aggaaagtcc
     1921 ttagagtttc atagacagct ggacaaggtc agccaatggt gtgaggcagg aatctacctc
     1981 ttggcttccc aagctgtaga caagtgccag tctcgagaag gggttgatat cgccttgaac
     2041 gacattgcga cattcctggg cacagtcaag gagtacccgt tgctcagccc caaggagttt
     2101 tacaacgagt ttgagttgct gctcaccctc gatgcaaagg ccaaagccca gaaagttttg
     2161 cagaggctgg atgatgtcca ggaaatattt cacaagaggc aagtgagtct gatgaaactg
     2221 gcagccaaac agactcgtcc agtgcaacct gtggccccac atcctgagtc ttcaccaaaa
     2281 tgggtgtcat caaaaaccag ccagccctcc acctcggtcc ctctagctcg tcctctgaga
     2341 acgtctgagg aaccttatac ggagacagag ttgaactccc ggggaaagga agatgatgag
     2401 actaaatttg aagtcaagag tgaagaaatc tttgaaagcc atcatgaaag ggggaaccct
     2461 gagctggagc agcaggccag gctcggagac ctttcccccc gcaggcgcat tatacgtgac
     2521 ttgcttgaga ctgaagagat ttacataaaa gagattaaaa gcataattga tggatatatc
     2581 actccaatgg attttatttg gctaaagcat ctaattccag atgttcttca gaataacaag
     2641 gactttctct ttgggaatat tagagaactt tacgaatttc acaacaggac ttttctaaaa
     2701 gagttggaaa agtgtgctga gaaccctgaa cttctggcac attgctttct caagagaaaa
     2761 gaagatcttc agatatattt taaataccat aagaatctgc cccgagctag ggcaatctgg
     2821 caagagtgtc aagactgcgc ctactttggg gtatgccagc gccaactgga tcacaatctc
     2881 cctcttttta agtatctcaa aggaccaagc cagagactta taaaatacca gatgctgttg
     2941 aagggtctgc tggatttcga gtctcctgaa gatatggaga tagacccagg tgaactagga
     3001 ggctcggcta aggatgggcc aaagagaacc aaagattcag cattctcaac tgaactacaa
     3061 caagctttgg cagtgataga ggatttgatc aagtcctgtg agttggctgt ggacctagca
     3121 gcagtgactg aatgtccgga cgatattgga aaactaggca agctgttgct gcacggccct
     3181 ttcagcgtct ggacaattca caaggatcgt tataaaatga aggatttgat tcgatttaaa
     3241 cccagccaga ggcaaatcta cctatttgaa aggggaatag tgttctgtaa gatacgaatg
     3301 gagcctgggg accagggatt atctcctcat tacagcttca agaagaccat gaagctgatg
     3361 acactttcaa ttcgccagct tggaaggggg agccatagaa agtttgagat tgccagtcga
     3421 aatggacttg agaaatacat cctgcaggca gcttcaaaag aaatcagaga ctgttggttt
     3481 tcagaaataa gtaaattatt gatggaacaa caaaataata tcaaagacca aggaaatcca
     3541 cagtttgaaa tgagcacgag caaaggcagt ggagcaggat ccggaccatg gattaaaaat
     3601 atggaaagag ctaccactag caaggaagac ccggcctcca gcacaggagg gattaaaggc
     3661 tgctccagca gggagtttag ctccatggac acctttgaag actgtgaagg cgcagaagac
     3721 atggaaaagg agagcagtgc tctgagtctc gcgggccttt tccagtcgga cgacagtcac
     3781 gaaacctgtt cctccaaatc tgctttcctg gagaggggag aaagcagcca gggagaaaaa
     3841 gaagaacgcg atgaggagga aacggcgacc cgcagcaccg aggaggagcg cgctggggcg
     3901 tccacgggcc ggctggctcc tgcgggggcg acggctggtt tccaggcgag ggcgctgcgc
     3961 ccgaggacct ccgcccagga gagctgacct ccctgcggac gcccccgctc ctcggctcca
     4021 gagcgcccgc attcccggga gaggcggtgt gggggcccgg gccctgccca gctacgcaga
     4081 aagcagccgg agcctcggcg gcggcagaaa ggggacaacc agggcctcct ccgaggagcc
     4141 cgaggggtgt cctgggtgcg cgcctagctc cgcacggggg acctcggagc tgctctaagg
     4201 cgcctgcaga ggcgagcaga gcccgcagcc cacgccttct cgaccgcgca cttcgatatt
     4261 cggagccggg caattctttg ctgcgtgggc ttctctgtgc tcccacggta ggatgtttag
     4321 tagcacccct ggcctctacc cactaggtgc caggaatgcg ccaccccatc ctccaccccg
     4381 cccccaaatc gtgacaatca aaaatgcctg cagacacgcc cgcatttctc cagggcgggg
     4441 tgggaatcgg ttgagagccg cttagcccga gccttggagg agccggagcc gctccaaccc
     4501 ggcgggggcc gcagactggg agctcccggt ccgcctccca gcatccctgc gagcgttcat
     4561 ggggtgttcg tgttagtgcc aagattgctt cgttgtagag agagttcgtt ccaagttact
     4621 ttctgaggta ttttatgtat cgatttatta gtttttaaat gagttttgtt agtttcagtt
     4681 gtattttatt tttagtttta ttagttgatt ttcatttctt tgtagctttc tggttattta
     4741 attttatagt ttcagttact ggtttatagt tactttattt tcaaagttat ttagttgttc
     4801 attttcagtt atttatatgt agttgttttg ttttaggagt tacagatgtt caaattaatt
     4861 tgcttggaat ttatttattt atttatttat ttatttttcg agacggagtc tcgctctgtc
     4921 gcccaggctg gagtgcagtg gtgtgatctc ggctcactgc aaaccgcctc ccgggttcac
     4981 gccattctcc tgcctcagcc tcctgagtag ctgggactac aggcgcccgc caccacgccc
     5041 ggctaatttt ttatttggat ttttagtaga gacggggttt ccccgtgtta gccaggatgg
     5101 tctcgatctc ctgacctcgt gatccccctg cctcggcctc ccaaagtgct gggattacag
     5161 gcgtgagcca ccgcgcccag ccggaattta tttttaatta tctttacaat tattatctga
     5221 gttatttacc ttcatagtta tttcacttta tcttattgga gtttttgagt tatttatttt
     5281 tacagtaact tatttgacta ttaaacactc actggaagtt catgaa
//
