LOCUS       NM_001112732            6427 bp    mRNA    linear   PRI 19-SEP-2024
DEFINITION  Homo sapiens MCF.2 cell line derived transforming sequence like
            (MCF2L), transcript variant 1, mRNA.
ACCESSION   NM_001112732
VERSION     NM_001112732.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6427)
  AUTHORS   Kong,W., Li,H., Xie,L., Cui,G., Gu,W., Zhang,H., Ma,W. and Zhou,Y.
  TITLE     LncRNA MCF2L-AS1 aggravates the malignant development of colorectal
            cancer via targeting miR-105-5p/RAB22A axis
  JOURNAL   BMC Cancer 21 (1), 1069 (2021)
   PUBMED   34592939
  REMARK    GeneRIF: LncRNA MCF2L-AS1 aggravates the malignant development of
            colorectal cancer via targeting miR-105-5p/RAB22A axis.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6427)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   3  (bases 1 to 6427)
  AUTHORS   Mao,J.H., Sui,Y.X., Ao,S., Wang,Y., Liu,Y. and Leng,H.
  TITLE     miR-140-3p exhibits repressive functions on preosteoblast viability
            and differentiation by downregulating MCF2L in osteoporosis
  JOURNAL   In Vitro Cell Dev Biol Anim 56 (1), 49-58 (2020)
   PUBMED   31732956
  REMARK    GeneRIF: miR-140-3p exhibits repressive functions on preosteoblast
            viability and differentiation by downregulating MCF2L in
            osteoporosis.
REFERENCE   4  (bases 1 to 6427)
  AUTHORS   Zhang,Y.J., Wen,C.L., Qin,Y.X., Tang,X.M., Shi,M.M., Shen,B.Y. and
            Fang,Y.
  TITLE     Establishment of a human primary pancreatic cancer mouse model to
            examine and investigate gemcitabine resistance
  JOURNAL   Oncol Rep 38 (6), 3335-3346 (2017)
   PUBMED   29039610
  REMARK    GeneRIF: When compared to the gemcitabine-sensitive cells, the
            gemcitabine-resistant cells had a higher level of MCF2L expression,
            suggesting that MCF2L plays an important role in gemcitabine
            resistance
REFERENCE   5  (bases 1 to 6427)
  AUTHORS   Shepherd,C., Skelton,A.J., Rushton,M.D., Reynard,L.N. and
            Loughlin,J.
  TITLE     Expression analysis of the osteoarthritis genetic susceptibility
            locus mapping to an intron of the MCF2L gene and marked by the
            polymorphism rs11842874
  JOURNAL   BMC Med Genet 16, 108 (2015)
   PUBMED   26584642
  REMARK    GeneRIF: MCF2L is subject to a cis-acting eQTL in synovial membrane
            that correlates with the OA association signal. This signal
            contains several functional SNPs that could account for the
            susceptibility and which therefore merit further investigation
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 6427)
  AUTHORS   Snyder,J.T., Worthylake,D.K., Rossman,K.L., Betts,L., Pruitt,W.M.,
            Siderovski,D.P., Der,C.J. and Sondek,J.
  TITLE     Structural basis for the selective activation of Rho GTPases by Dbl
            exchange factors
  JOURNAL   Nat Struct Biol 9 (6), 468-475 (2002)
   PUBMED   12006984
REFERENCE   7  (bases 1 to 6427)
  AUTHORS   Rossman,K.L., Worthylake,D.K., Snyder,J.T., Siderovski,D.P.,
            Campbell,S.L. and Sondek,J.
  TITLE     A crystallographic view of interactions between Dbs and Cdc42: PH
            domain-assisted guanine nucleotide exchange
  JOURNAL   EMBO J 21 (6), 1315-1326 (2002)
   PUBMED   11889037
REFERENCE   8  (bases 1 to 6427)
  AUTHORS   Harrington,A.W., Kim,J.Y. and Yoon,S.O.
  TITLE     Activation of Rac GTPase by p75 is necessary for c-jun N-terminal
            kinase-mediated apoptosis
  JOURNAL   J Neurosci 22 (1), 156-166 (2002)
   PUBMED   11756498
REFERENCE   9  (bases 1 to 6427)
  AUTHORS   Whitehead,I.P., Lambert,Q.T., Glaven,J.A., Abe,K., Rossman,K.L.,
            Mahon,G.M., Trzaskos,J.M., Kay,R., Campbell,S.L. and Der,C.J.
  TITLE     Dependence of Dbl and Dbs transformation on MEK and NF-kappaB
            activation
  JOURNAL   Mol Cell Biol 19 (11), 7759-7770 (1999)
   PUBMED   10523665
REFERENCE   10 (bases 1 to 6427)
  AUTHORS   Horii,Y., Beeler,J.F., Sakaguchi,K., Tachibana,M. and Miki,T.
  TITLE     A novel oncogene, ost, encodes a guanine nucleotide exchange factor
            that potentially links Rho and Rac signaling pathways
  JOURNAL   EMBO J 13 (20), 4776-4786 (1994)
   PUBMED   7957046
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL356740.19, AK295670.1,
            AL137002.19 and AW241629.1.
            
            On Nov 22, 2018 this sequence version replaced NM_001112732.2.
            
            Summary: This gene encodes a guanine nucleotide exchange factor
            that interacts specifically with the GTP-bound Rac1 and plays a
            role in the Rho/Rac signaling pathways. A variant in this gene was
            associated with osteoarthritis. Alternative splicing results in
            multiple transcript variants. [provided by RefSeq, Mar 2016].
            
            Transcript Variant: This variant (1) encodes isoform a.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            CCDS Note: The coding region has been updated to represent an
            alternative splicing pattern that is more supported by the
            available transcript data.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660803.220663.1,
                                           SRR1660805.67443.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968189, SAMEA1968540
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000535094.7/ ENSP00000440374.2
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-165               AL356740.19        103255-103419
            166-2236            AK295670.1         1-2071
            2237-2237           AL137002.19        9544-9544
            2238-2580           AK295670.1         2073-2415
            2581-2581           AL137002.19        11919-11919
            2582-3230           AK295670.1         2417-3065
            3231-3231           AL137002.19        19268-19268
            3232-3645           AK295670.1         3067-3480
            3646-6250           AL137002.19        21604-24208
            6251-6424           AW241629.1         8-181               c
            6425-6427           AL137002.19        24383-24385
FEATURES             Location/Qualifiers
     source          1..6427
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="13"
                     /map="13q34"
     gene            1..6427
                     /gene="MCF2L"
                     /gene_synonym="ARHGEF14; DBS; OST"
                     /note="MCF.2 cell line derived transforming sequence like"
                     /db_xref="GeneID:23263"
                     /db_xref="HGNC:HGNC:14576"
                     /db_xref="MIM:609499"
     exon            1..245
                     /gene="MCF2L"
                     /gene_synonym="ARHGEF14; DBS; OST"
                     /inference="alignment:Splign:2.1.0"
     CDS             167..3544
                     /gene="MCF2L"
                     /gene_synonym="ARHGEF14; DBS; OST"
                     /note="isoform a is encoded by transcript variant 1; MCF2
                     transforming sequence-like protein; guanine nucleotide
                     exchange factor DBS; DBL's big sister"
                     /codon_start=1
                     /product="guanine nucleotide exchange factor DBS isoform
                     a"
                     /protein_id="NP_001106203.2"
                     /db_xref="CCDS:CCDS45070.2"
                     /db_xref="GeneID:23263"
                     /db_xref="HGNC:HGNC:14576"
                     /db_xref="MIM:609499"
                     /translation="MRFWLRTEEMALEEMVQRLNAVSKHTDEIMHQDIVPLCAADIQD
                     QLKKRFAYLSGGRGQDGSPVITFPDYPAFSEIPDKEFQNVMTYLTSIPSLQDAGIGFI
                     LVIDRRRDKWTSVKASVLRIAASFPANLQLVLVLRPTGFFQRTLSDIAFKFNRDDFKM
                     KVPVIMLSSVPDLHGYIDKSQLTEDLGGTLDYCHSRWLCQRTAIESFALMVKQTAQML
                     QSFGTELAETELPNDVQSTSSVLCAHTEKKDKAKEDLRLALKEGHSVLESLRELQAEG
                     SEPSVNQDQLDNQATVQRLLAQLNETEAAFDEFWAKHQQKLEQCLQLRHFEQGFREVK
                     AILDAASQKIATFTDIGNSLAHVEHLLRDLASFEEKSGVAVERARALSLDGEQLIGNK
                     HYAVDSIRPKCQELRHLCDQFSAEIARRRGLLSKSLELHRRLETSMKWCDEGIYLLAS
                     QPVDKCQSQDGAEAALQEIEKFLETGAENKIQELNAIYKEYESILNQDLMEHVRKVFQ
                     KQASMEEVFHRRQASLKKLAARQTRPVQPVAPRPEALAKSPCPSPGIRRGSENSSSEG
                     GALRRGPYRRAKSEMSESRQGRGSAGEEEESLAILRRHVMSELLDTERAYVEELLCVL
                     EGYAAEMDNPLMAHLLSTGLHNKKDVLFGNMEEIYHFHNRIFLRELENYTDCPELVGR
                     CFLERMEDFQIYEKYCQNKPRSESLWRQCSDCPFFQECQRKLDHKLSLDSYLLKPVQR
                     ITKYQLLLKEMLKYSRNCEGAEDLQEALSSILGILKAVNDSMHLIAITGYDGNLGDLG
                     KLLMQGSFSVWTDHKRGHTKVKELARFKPMQRHLFLHEKAVLFCKKREENGEGYEKAP
                     SYSYKQSLNMAAVGITENVKGDAKKFEIWYNAREEVYIVQAPTPEIKAAWVNEIRKVL
                     TSQLQACREASQHRALEQSQSLPLPAPTSTSPSRGNSRNIKKLEERKTDPLSLEGYVS
                     SAPLTKPPEKGKGWSKTSHSLEAPEDDGGWSSAEEQINSSDAEEDGGLGPKKLVPGKY
                     TVVADHEKGGPDALRVRSGDVVELVQEGDEGLWYVRDPTTGKEGWVPASSLSVRLGPS
                     GSAQCLSSSESSPGSAVLSNSSSCSEGGQAPFSDLQG"
     exon            246..329
                     /gene="MCF2L"
                     /gene_synonym="ARHGEF14; DBS; OST"
                     /inference="alignment:Splign:2.1.0"
     exon            330..444
                     /gene="MCF2L"
                     /gene_synonym="ARHGEF14; DBS; OST"
                     /inference="alignment:Splign:2.1.0"
     exon            445..535
                     /gene="MCF2L"
                     /gene_synonym="ARHGEF14; DBS; OST"
                     /inference="alignment:Splign:2.1.0"
     exon            536..655
                     /gene="MCF2L"
                     /gene_synonym="ARHGEF14; DBS; OST"
                     /inference="alignment:Splign:2.1.0"
     exon            656..772
                     /gene="MCF2L"
                     /gene_synonym="ARHGEF14; DBS; OST"
                     /inference="alignment:Splign:2.1.0"
     exon            773..922
                     /gene="MCF2L"
                     /gene_synonym="ARHGEF14; DBS; OST"
                     /inference="alignment:Splign:2.1.0"
     exon            923..1047
                     /gene="MCF2L"
                     /gene_synonym="ARHGEF14; DBS; OST"
                     /inference="alignment:Splign:2.1.0"
     exon            1048..1162
                     /gene="MCF2L"
                     /gene_synonym="ARHGEF14; DBS; OST"
                     /inference="alignment:Splign:2.1.0"
     exon            1163..1282
                     /gene="MCF2L"
                     /gene_synonym="ARHGEF14; DBS; OST"
                     /inference="alignment:Splign:2.1.0"
     exon            1283..1474
                     /gene="MCF2L"
                     /gene_synonym="ARHGEF14; DBS; OST"
                     /inference="alignment:Splign:2.1.0"
     exon            1475..1666
                     /gene="MCF2L"
                     /gene_synonym="ARHGEF14; DBS; OST"
                     /inference="alignment:Splign:2.1.0"
     exon            1667..1826
                     /gene="MCF2L"
                     /gene_synonym="ARHGEF14; DBS; OST"
                     /inference="alignment:Splign:2.1.0"
     exon            1827..1900
                     /gene="MCF2L"
                     /gene_synonym="ARHGEF14; DBS; OST"
                     /inference="alignment:Splign:2.1.0"
     exon            1901..1974
                     /gene="MCF2L"
                     /gene_synonym="ARHGEF14; DBS; OST"
                     /inference="alignment:Splign:2.1.0"
     exon            1975..2041
                     /gene="MCF2L"
                     /gene_synonym="ARHGEF14; DBS; OST"
                     /inference="alignment:Splign:2.1.0"
     exon            2042..2157
                     /gene="MCF2L"
                     /gene_synonym="ARHGEF14; DBS; OST"
                     /inference="alignment:Splign:2.1.0"
     exon            2158..2227
                     /gene="MCF2L"
                     /gene_synonym="ARHGEF14; DBS; OST"
                     /inference="alignment:Splign:2.1.0"
     exon            2228..2320
                     /gene="MCF2L"
                     /gene_synonym="ARHGEF14; DBS; OST"
                     /inference="alignment:Splign:2.1.0"
     exon            2321..2413
                     /gene="MCF2L"
                     /gene_synonym="ARHGEF14; DBS; OST"
                     /inference="alignment:Splign:2.1.0"
     exon            2414..2539
                     /gene="MCF2L"
                     /gene_synonym="ARHGEF14; DBS; OST"
                     /inference="alignment:Splign:2.1.0"
     exon            2540..2761
                     /gene="MCF2L"
                     /gene_synonym="ARHGEF14; DBS; OST"
                     /inference="alignment:Splign:2.1.0"
     exon            2762..2854
                     /gene="MCF2L"
                     /gene_synonym="ARHGEF14; DBS; OST"
                     /inference="alignment:Splign:2.1.0"
     exon            2855..2933
                     /gene="MCF2L"
                     /gene_synonym="ARHGEF14; DBS; OST"
                     /inference="alignment:Splign:2.1.0"
     exon            2934..3000
                     /gene="MCF2L"
                     /gene_synonym="ARHGEF14; DBS; OST"
                     /inference="alignment:Splign:2.1.0"
     exon            3001..3119
                     /gene="MCF2L"
                     /gene_synonym="ARHGEF14; DBS; OST"
                     /inference="alignment:Splign:2.1.0"
     exon            3120..3241
                     /gene="MCF2L"
                     /gene_synonym="ARHGEF14; DBS; OST"
                     /inference="alignment:Splign:2.1.0"
     exon            3242..3354
                     /gene="MCF2L"
                     /gene_synonym="ARHGEF14; DBS; OST"
                     /inference="alignment:Splign:2.1.0"
     exon            3355..3458
                     /gene="MCF2L"
                     /gene_synonym="ARHGEF14; DBS; OST"
                     /inference="alignment:Splign:2.1.0"
     exon            3459..6427
                     /gene="MCF2L"
                     /gene_synonym="ARHGEF14; DBS; OST"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5212..5217
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MCF2L"
                     /gene_synonym="ARHGEF14; DBS; OST"
                     /note="hexamer: AATAAA"
     polyA_site      5233
                     /gene="MCF2L"
                     /gene_synonym="ARHGEF14; DBS; OST"
                     /note="major polyA site"
     regulatory      6354..6359
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MCF2L"
                     /gene_synonym="ARHGEF14; DBS; OST"
                     /note="hexamer: AATAAA"
     polyA_site      6379
                     /gene="MCF2L"
                     /gene_synonym="ARHGEF14; DBS; OST"
     regulatory      6399..6404
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MCF2L"
                     /gene_synonym="ARHGEF14; DBS; OST"
                     /note="hexamer: AATAAA"
     polyA_site      6419
                     /gene="MCF2L"
                     /gene_synonym="ARHGEF14; DBS; OST"
ORIGIN      
        1 gcggaaccaa tcctgggcag ggaggcggcg gctggaggct gaaagcgctg ccgtggcccc
       61 ctccccgcct ccgccgcgcc ccctccgcac tcgcacggcc ccacccgcag gcgccccccg
      121 tgcggaggaa gcggatctgc caggatcatt tttgttgtgt cggaggatga ggttttggct
      181 gaggactgaa gagatggcct tggaagaaat ggtgcagaga ttaaatgcgg tttccaagca
      241 cacggatgaa atcatgcacc aggacatcgt cccgctctgt gctgccgaca tccaggacca
      301 gctaaagaag cgctttgctt acctgtccgg tgggcggggg caggacggaa gcccggttat
      361 caccttccct gactacccgg ccttcagcga gattccggac aaggagttcc agaatgtcat
      421 gacctacctc accagcatcc ccagcctgca ggacgctggc atcggattca tcctggtgat
      481 agaccggcga cgggacaaat ggacctccgt gaaggcgtcc gtcctgcgca tcgcagcatc
      541 tttcccggca aacctgcagc tcgtcctcgt gcttcgcccg acgggttttt tccaaaggac
      601 tctctccgac atcgctttca aattcaatag agatgacttt aagatgaagg tgccggtcat
      661 aatgctgagc tccgtaccag acttacacgg ttacatcgat aagtcgcagc tgaccgagga
      721 cctgggtggg accctggact actgccactc ccggtggctg tgccagcgca cggccatcga
      781 aagtttcgcc ctcatggtga agcagacggc tcagatgctg cagtccttcg ggaccgagct
      841 ggctgaaaca gagctgccca atgacgtcca gtcgacaagc tcagtgctgt gtgcgcacac
      901 agagaagaag gacaaggcga aggaggattt gaggctggca ctgaaagagg ggcacagtgt
      961 cctggagagc ctcagggagc tgcaggctga gggctcagag cccagtgtga accaggacca
     1021 gcttgacaac caggccaccg tgcagaggct cctggcccag ctgaacgaaa ccgaggctgc
     1081 cttcgatgag ttctgggcaa agcatcagca gaaactggag cagtgtctgc agctccggca
     1141 ctttgagcag ggcttccggg aggtcaaagc catcttggac gcagcgtccc agaagatagc
     1201 aaccttcaca gacatcggca acagcctggc gcatgtggag cacctgctga gggacctggc
     1261 cagcttcgag gagaaatcag gcgtggccgt ggagagggcc cgggccctgt ctctggacgg
     1321 cgagcagctc attgggaaca agcactacgc ggtagactcc atccgcccaa agtgccagga
     1381 gctccggcac ctctgtgacc agttctctgc ggagatcgca aggaggaggg ggctgctcag
     1441 caagtccctg gagctgcacc gccgcctgga gacgtccatg aagtggtgtg atgaagggat
     1501 ttacctgctg gcctcacaac ctgtggacaa gtgccagtcc caggacggcg cggaggctgc
     1561 cctccaggaa atcgagaagt ttttggagac cggtgcggaa aataagatcc aggagctcaa
     1621 cgcgatttac aaggaatacg aatccatcct caaccaagat ctcatggagc acgtgcgaaa
     1681 ggtcttccag aagcaggcaa gcatggagga ggtgttccac cgcaggcagg ccagcctgaa
     1741 gaagctggcg gccaggcaga cgcggcccgt gcagccggtg gcccccagac ccgaggcact
     1801 ggcaaagtcg ccctgcccct ccccaggcat tcggcgaggc tctgagaact ccagctccga
     1861 gggcggtgcg ctccggagag ggccctaccg gagggccaag agtgagatga gtgagagccg
     1921 gcagggccgc ggctcagcgg gggaggagga ggaaagcctg gccatcctgc gcaggcacgt
     1981 gatgagcgag ctcctggaca cagaacgggc ctacgtggag gagctgctgt gcgtcctgga
     2041 gggctacgcc gcggagatgg ataacccact gatggctcac ctcctgtcaa caggccttca
     2101 caacaagaag gatgttttgt ttggaaacat ggaggaaatc tatcacttcc acaacaggat
     2161 attcctcagg gagctggaaa actacactga ctgcccagaa ctggttggaa gatgctttct
     2221 ggagaggatg gaagatttcc agatctatga gaagtactgt cagaacaagc cccgctctga
     2281 gagcctgtgg agacagtgct ccgactgccc gtttttccag gaatgccaga gaaagctgga
     2341 ccacaagctg agcctggact cctacctgct gaagccagtg cagaggatca ccaagtacca
     2401 gctgctgctc aaggaaatgc tgaaatacag caggaactgc gagggggctg aggacctgca
     2461 ggaggcgctg agctccatcc tgggcatcct gaaggccgtg aacgactcca tgcacctcat
     2521 cgctatcacc ggctatgacg ggaatctcgg cgacctgggc aagctgctga tgcagggctc
     2581 gttcagcgtc tggaccgacc acaagagggg ccacaccaag gtgaaggagc tggccaggtt
     2641 caagcccatg cagcggcacc tgttcctgca cgagaaggca gtgctcttct gcaagaagag
     2701 ggaggagaat ggggaggggt atgagaaagc tccctcctac agctacaagc agtccttaaa
     2761 catggctgcc gttggcatta cggagaacgt gaagggagat gctaagaagt tcgagatctg
     2821 gtacaacgcg cgcgaggagg tctacatcgt ccaggcgcca actcctgaga ttaaagccgc
     2881 gtgggtgaat gaaattcgga aagtgctgac cagccagctg caggcttgta gagaagccag
     2941 ccagcaccgg gcgctggagc agtcacagag cctgcccctg ccggccccga ccagcaccag
     3001 tccctcaaga ggaaactcaa ggaacatcaa gaagctggaa gaaaggaaaa cagaccccct
     3061 aagcctggag ggatacgtca gctcagcgcc actgacaaag ccccccgaaa agggcaaagg
     3121 ttggagcaaa acgtcccact cactggaggc acctgaggac gacgggggct ggtcaagtgc
     3181 agaggagcag attaactcgt ccgacgcaga ggaggacggc gggttgggcc ccaagaagct
     3241 ggttccaggt aaatacacgg tcgtggcgga ccacgagaag ggaggccccg atgcgctgcg
     3301 cgtgaggagc ggggacgtgg tggagctggt gcaggagggc gacgagggcc tctggtacgt
     3361 cagggacccg accactggca aggagggctg ggtgccggcc agcagcctgt ccgtccggct
     3421 cggcccgtcc ggctcggccc agtgcctgag cagctcagag tcgagcccgg ggtcggccgt
     3481 gctgagcaac tcgtccagct gcagcgaggg cggccaggcc cccttctccg acctgcaggg
     3541 gtagcgcggc ctcggcgccg gagacccgcg cgctgtctgg ggctgcggtg gcgtggggag
     3601 ggcgcggccc ccggacgccc cgaggaaggg gcacctcacc gcccccaccc agagcgcctg
     3661 gccgtgcggg ctgcagagga cccctccggg gcagaggcag gttccacgga agaccccggc
     3721 ccgctggggc ttccccggag actccagagc ccacagagga ggggccgcag ggaacagccc
     3781 cgggcggcag gcgccgggca gcggcatctc gtcctggctc caccgtgctg cttctgcctc
     3841 tggacggtgc tttcagggga cgcgcggacc gtggtggagc tgcttccgga gaagtggagg
     3901 atcctctggc caacggcctg aggagagcgg ggcacggggt ctctttagct tttacaagtt
     3961 ttaggatttt ttcaagcagg gatcaatccc gtggccattt tttgtggtac tttggcctca
     4021 attcttcacc aggaatcact gtgtttacat gaaatgacaa tttgatactg tatttgatag
     4081 aaaactattt ttttgttacc ggggtttaca tagaagcacg ttgtttatac cactaagtga
     4141 ctttgggggg gctctcccat ggaaacggat ggcactccct gaagctccct ggtcacaggt
     4201 ggatgaaaac gtgtccgtgg gtgacatcag gtggtgtctc caccaccaaa agcagttaga
     4261 agccaaggag attcctttat ctacctaggg ttcattttca aaagaaaatt taaactataa
     4321 tttaaacaat taacgttctt ttctacaaaa aaaatgcagg gacttgattt ttttaaagag
     4381 cttcactgaa ttaggatatt tttattgctt ttaaagaaaa tacaaagatg cagtttctgc
     4441 agggtgtggc gtggaccagt gctgccgacc atagctcaga gagccctgcc cctgcctcac
     4501 tgcactgcag cctcctcgga ggccgcacct ccactccact ccccacgcgc cccctgcctc
     4561 ccacccaggt ccacctgcca cctggtgacc accttgagta cagaagtgaa agtggggaga
     4621 gtattttatt caagtcacag cagaactgga aaaaaactct tctgttttac caacttcttg
     4681 tgtttcagaa acatattctg ttcaaaactt ttgaagccct ttcggtgtct agtctgcaga
     4741 tgtttttgta tgtgtgcacc tctgaccatg tgtgtacata tgtgtcttgc tggaaaggac
     4801 atattcgctg tccccgtgct gctgggaggg ccgcctcaca gcctcacggt tcccagcccc
     4861 agcacagtgg aggcaggcgt ggctgcattc ccctcacgct accctcccag cggcttgtag
     4921 ccgtcactgg ccagacctcc agggtgcgga atcaaatagg aagcatgcag agactcggca
     4981 gcttttcctc tgatgtgtaa gttatttgga acgcgtgctg tgtcccgcga tgtccctgat
     5041 gtactgtgca ggcgcggtgc ctccgtctcg tcgcacagct gcgcgccctt gtgtgaccct
     5101 ccccataaag gcactttaca gcttcatgtt tcatccactg tcactttttt ttaactgctg
     5161 atgtaaatgg aattttaaaa gcagagttct ttattgtatg gatgacgttt gaataaatat
     5221 cagcaactcc tgccatctgc ctttgtctgt caagacacag aacgtctcag cagtcggggt
     5281 ttccagggcc gcagtgcact gtgcttgcac atggtaagtc attgttggga cggaaaagaa
     5341 gccggcagtg ggcagggccc agcgtgcggc tcaggcaccg agcaaccgct ttgctttctt
     5401 ctgtcagacg gcgatgatga caaaatagca acaaggttgt gcgtgtcaga aacgcaaagg
     5461 cagcagagga agcgtagtgg aaccattaca gaatcacaat gcagccgaca ctctccagac
     5521 cagaaaaggg agcataaaga aagggtattg atccaataga agaagggaag ggtggagaaa
     5581 ggggaaagca tggttaacag gaaacaacat gtaacggaag agacagccca gatgtgtctg
     5641 gctcacaaca gacgtgatca tgttatgctg gcctggaaga gcatcggatc agacgtgaca
     5701 agtcactgct tagagaccat caagcaaatt tatatataga ttggagattt aaaataaaag
     5761 aagacagaac agacaaacac cataagaaag ctggtgtagc agtatcgatg acctgaaatg
     5821 ggattcagga cagttcatag agtaaagggg gctgcgtggc aatcaggaac tcataagcca
     5881 ctgactataa agctcaaaac acagcaaagt tggcagtcgg cagacagcaa tgttgactgt
     5941 catgaaaagt gatccctgtt tgcccctaaa cgtagagaaa tctgcgttat tttccagcac
     6001 acatggagca caaacaaaat atttgcaaaa caatgggaag atcattgaaa cactgtttgg
     6061 caatttaaaa gcttgtttct aactcacggg atgcgggcag tctgctctct agaactggac
     6121 agcgtgcaca gagccacggg agggagcagc cacggccagc tcagattggt gtcgacagct
     6181 tagtggtgtc tgattttata catgacaaaa tgaacgagtt aaccatttaa gccaaaaaaa
     6241 taagactagc gtaacccaaa gaaagtattt aaatacttct gtcaattagg acagttgaga
     6301 aaagagaata acaaaatcaa aagcaaaact caaactttgt acctgaaaaa tctaataaaa
     6361 ctgactaatt tatagaaaac ctaagaaact ccatatcaaa taaaaaattt taaatatgag
     6421 agaacca
//
