LOCUS       NM_001085377            8295 bp    mRNA    linear   PRI 19-SEP-2024
DEFINITION  Homo sapiens MCC regulator of WNT signaling pathway (MCC),
            transcript variant 1, mRNA.
ACCESSION   NM_001085377
VERSION     NM_001085377.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 8295)
  AUTHORS   Xiao,N., Cao,X., Liu,Z. and Han,Y.
  TITLE     Two germline mutations can serve as genetic susceptibility
            screening makers for a lung adenocarcinoma family
  JOURNAL   J Cancer Res Clin Oncol 149 (9), 6541-6548 (2023)
   PUBMED   36781503
  REMARK    GeneRIF: Two germline mutations can serve as genetic susceptibility
            screening makers for a lung adenocarcinoma family.
REFERENCE   2  (bases 1 to 8295)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   3  (bases 1 to 8295)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   4  (bases 1 to 8295)
  AUTHORS   Buljan,M., Ciuffa,R., van Drogen,A., Vichalkovski,A., Mehnert,M.,
            Rosenberger,G., Lee,S., Varjosalo,M., Pernas,L.E., Spegg,V.,
            Snijder,B., Aebersold,R. and Gstaiger,M.
  TITLE     Kinase Interaction Network Expands Functional and Disease Roles of
            Human Kinases
  JOURNAL   Mol Cell 79 (3), 504-520 (2020)
   PUBMED   32707033
REFERENCE   5  (bases 1 to 8295)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   6  (bases 1 to 8295)
  AUTHORS   Curtis,L.J., Bubb,V.J., Gledhill,S., Morris,R.G., Bird,C.C. and
            Wyllie,A.H.
  TITLE     Loss of heterozygosity of MCC is not associated with mutation of
            the retained allele in sporadic colorectal cancer
  JOURNAL   Hum Mol Genet 3 (3), 443-446 (1994)
   PUBMED   8012355
REFERENCE   7  (bases 1 to 8295)
  AUTHORS   Lindgren,V., Bryke,C.R., Ozcelik,T., Yang-Feng,T.L. and Francke,U.
  TITLE     Phenotypic, cytogenetic, and molecular studies of three patients
            with constitutional deletions of chromosome 5 in the region of the
            gene for familial adenomatous polyposis
  JOURNAL   Am J Hum Genet 50 (5), 988-997 (1992)
   PUBMED   1315124
  REMARK    Review article
REFERENCE   8  (bases 1 to 8295)
  AUTHORS   Nishisho,I., Nakamura,Y., Miyoshi,Y., Miki,Y., Ando,H., Horii,A.,
            Koyama,K., Utsunomiya,J., Baba,S. and Hedge,P.
  TITLE     Mutations of chromosome 5q21 genes in FAP and colorectal cancer
            patients
  JOURNAL   Science 253 (5020), 665-669 (1991)
   PUBMED   1651563
REFERENCE   9  (bases 1 to 8295)
  AUTHORS   Kinzler,K.W., Nilbert,M.C., Vogelstein,B., Bryan,T.M., Levy,D.B.,
            Smith,K.J., Preisinger,A.C., Hamilton,S.R., Hedge,P., Markham,A. et
            al.
  TITLE     Identification of a gene located at chromosome 5q21 that is mutated
            in colorectal cancers
  JOURNAL   Science 251 (4999), 1366-1370 (1991)
   PUBMED   1848370
REFERENCE   10 (bases 1 to 8295)
  AUTHORS   Hoshino,Y., Horikawa,I., Oshimura,M. and Yuasa,Y.
  TITLE     Normal human chromosome 5, on which a familial adenomatous
            polyposis gene is located, has tumor suppressive activity
  JOURNAL   Biochem Biophys Res Commun 174 (1), 298-304 (1991)
   PUBMED   1846539
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC093208.2, AC079465.5,
            AC126917.1, AC106750.3 and AC008536.7.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_001085377.1.
            
            Summary: This gene is a candidate colorectal tumor suppressor gene
            that is thought to negatively regulate cell cycle progression. The
            orthologous gene in the mouse expresses a phosphoprotein associated
            with the plasma membrane and membrane organelles, and
            overexpression of the mouse protein inhibits entry into S phase.
            Multiple transcript variants encoding different isoforms have been
            found for this gene. [provided by RefSeq, Jul 2008].
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data because no single transcript was available
            for the full length of the gene. The extent of this transcript is
            supported by transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK128596.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000408903.7/ ENSP00000386227.3
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-209               AC093208.2         67593-67801
            210-454             AC079465.5         9552-9796
            455-666             AC079465.5         54034-54245
            667-780             AC126917.1         26084-26197         c
            781-923             AC126917.1         17993-18135         c
            924-1066            AC106750.3         88654-88796         c
            1067-1230           AC106750.3         70162-70325         c
            1231-1437           AC106750.3         67709-67915         c
            1438-1584           AC106750.3         51134-51280         c
            1585-1674           AC106750.3         50071-50160         c
            1675-1823           AC106750.3         48830-48978         c
            1824-1964           AC106750.3         37064-37204         c
            1965-2068           AC106750.3         34050-34153         c
            2069-2252           AC106750.3         29954-30137         c
            2253-2487           AC106750.3         19695-19929         c
            2488-2694           AC106750.3         15063-15269         c
            2695-2795           AC106750.3         9500-9600           c
            2796-2918           AC008536.7         169241-169363       c
            2919-8295           AC008536.7         162413-167789       c
FEATURES             Location/Qualifiers
     source          1..8295
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="5"
                     /map="5q22.2"
     gene            1..8295
                     /gene="MCC"
                     /gene_synonym="MCC1"
                     /note="MCC regulator of WNT signaling pathway"
                     /db_xref="GeneID:4163"
                     /db_xref="HGNC:HGNC:6935"
                     /db_xref="MIM:159350"
     exon            1..209
                     /gene="MCC"
                     /gene_synonym="MCC1"
                     /inference="alignment:Splign:2.1.0"
     CDS             40..3099
                     /gene="MCC"
                     /gene_synonym="MCC1"
                     /note="isoform 1 is encoded by transcript variant 1;
                     mutated in colorectal cancers; MCC, WNT signaling pathway
                     regulator"
                     /codon_start=1
                     /product="colorectal mutant cancer protein isoform 1"
                     /protein_id="NP_001078846.2"
                     /db_xref="CCDS:CCDS43351.1"
                     /db_xref="GeneID:4163"
                     /db_xref="HGNC:HGNC:6935"
                     /db_xref="MIM:159350"
                     /translation="MMAAAAAAAAGSSSSGGGGGGSGSSSSSSDTSSTGEEERMRRLF
                     QTCDGDGDGYISRNDLLMVCRQLNMEESVAEIMNQLGADENGKISFQDFTRCRMQLVR
                     EIRKEEVDLSAKSDNSCTKKLRDRIASWPTSSDNSLGALSAARESWEYDSGARDLQSP
                     DVQSQSALQKLLEYGGSSLHQQAALHKLLTQSPHIGNSVGGSYLELANTLHSAALASL
                     KGDIVELNKRLQQTERERDLLEKKLAKAQCEQSHLMREHEDVQERTTLRYEERITELH
                     SVIAELNKKIDRLQGTTIREEDEYSELRSELSQSQHEVNEDSRSMDQDQTSVSIPENQ
                     STMVTADMDNCSDLNSELQRVLTGLENVVCGRKKSSCSLSVAEVDKHIEQLTTASEHC
                     DLAIKTVEEIEGVLGRDLYPNLAEERSRWEKELAGLREENESLTAMLCSKEEELNRTK
                     ATMNAIREERDRLRRRVRELQTRLQSVQATGPSSPGRLTSTNRPINPSTGELSTSSSS
                     NDIPIAKIAERVKLSKTRSESSSSDRPVLGSEISSIGVSSSVAEHLAHSLQDCSNIQE
                     IFQTLYSHGSAISESKIREFEVETERLNSRIEHLKSQNDLLTITLEECKSNAERMSML
                     VGKYESNATALRLALQYSEQCIEAYELLLALAESEQSLILGQFRAAGVGSSPGDQSGD
                     ENITQMLKRAHDCRKTAENAAKALLMKLDGSCGGAFAVAGCSVQPWESLSSNSHTSTT
                     SSTASSCDTEFTKEDEQRLKDYIQQLKNDRAAVKLTMLELESIHIDPLSYDVKPRGDS
                     QRLDLENAVLMQELMAMKEEMAELKAQLYLLEKEKKALELKLSTREAQEQAYLVHIEH
                     LKSEVEEQKEQRMRSLSSTSSGSKDKPGKECADAASPALSLAELRTTCSENELAAEFT
                     NAIRREKKLKARVQELVSALERLTKSSEIRHQQSAEFVNDLKRANSNLVAAYEKAKKK
                     HQNKLKKLESQMMAMVERHETQVRMLKQRIALLEEENSRPHTNETSL"
     exon            210..454
                     /gene="MCC"
                     /gene_synonym="MCC1"
                     /inference="alignment:Splign:2.1.0"
     exon            455..666
                     /gene="MCC"
                     /gene_synonym="MCC1"
                     /inference="alignment:Splign:2.1.0"
     exon            667..780
                     /gene="MCC"
                     /gene_synonym="MCC1"
                     /inference="alignment:Splign:2.1.0"
     exon            781..923
                     /gene="MCC"
                     /gene_synonym="MCC1"
                     /inference="alignment:Splign:2.1.0"
     exon            924..1066
                     /gene="MCC"
                     /gene_synonym="MCC1"
                     /inference="alignment:Splign:2.1.0"
     exon            1067..1230
                     /gene="MCC"
                     /gene_synonym="MCC1"
                     /inference="alignment:Splign:2.1.0"
     exon            1231..1437
                     /gene="MCC"
                     /gene_synonym="MCC1"
                     /inference="alignment:Splign:2.1.0"
     exon            1438..1584
                     /gene="MCC"
                     /gene_synonym="MCC1"
                     /inference="alignment:Splign:2.1.0"
     exon            1585..1674
                     /gene="MCC"
                     /gene_synonym="MCC1"
                     /inference="alignment:Splign:2.1.0"
     exon            1675..1823
                     /gene="MCC"
                     /gene_synonym="MCC1"
                     /inference="alignment:Splign:2.1.0"
     exon            1824..1964
                     /gene="MCC"
                     /gene_synonym="MCC1"
                     /inference="alignment:Splign:2.1.0"
     exon            1965..2068
                     /gene="MCC"
                     /gene_synonym="MCC1"
                     /inference="alignment:Splign:2.1.0"
     exon            2069..2252
                     /gene="MCC"
                     /gene_synonym="MCC1"
                     /inference="alignment:Splign:2.1.0"
     exon            2253..2487
                     /gene="MCC"
                     /gene_synonym="MCC1"
                     /inference="alignment:Splign:2.1.0"
     exon            2488..2694
                     /gene="MCC"
                     /gene_synonym="MCC1"
                     /inference="alignment:Splign:2.1.0"
     exon            2695..2795
                     /gene="MCC"
                     /gene_synonym="MCC1"
                     /inference="alignment:Splign:2.1.0"
     exon            2796..2918
                     /gene="MCC"
                     /gene_synonym="MCC1"
                     /inference="alignment:Splign:2.1.0"
     exon            2919..8295
                     /gene="MCC"
                     /gene_synonym="MCC1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      8276..8281
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MCC"
                     /gene_synonym="MCC1"
                     /note="hexamer: AATAAA"
     polyA_site      8295
                     /gene="MCC"
                     /gene_synonym="MCC1"
                     /note="major polyA site"
ORIGIN      
        1 ggtcgcgcgt actcctcctc ccaagtaggg agcgggcgca tgatggcggc cgcggcggca
       61 gcggctgcgg ggagctccag cagcggcggc ggcggcggcg gcagcggcag cagcagcagc
      121 agcagcgaca cgtccagcac cggcgaggag gagaggatgc ggcgcctctt ccagacgtgc
      181 gacggcgacg gggacggata catcagcaga aatgacttgc taatggtctg tcgccagctg
      241 aatatggaag agtctgtggc tgagatcatg aaccagttgg gagcagatga aaatgggaag
      301 atttcctttc aggatttcac aagatgccgc atgcagcttg ttcgagaaat taggaaggag
      361 gaagtagatc tttctgcaaa gtcagacaac tcctgtacaa agaagctgag ggatagaatt
      421 gcttcctggc ccacgagcag tgacaacagt ttgggtgcct tatcagcagc cagggagagc
      481 tgggaatacg actctggtgc cagggacctc cagagcccgg atgtgcagag ccagtcagcc
      541 ctccagaagc tgctcgagta tggcggaagc tctttgcatc agcaggctgc tctccacaaa
      601 ctgctcacac agtccccgca cattggcaac tctgtaggag gaagctatct agagctggcc
      661 aacacactcc attcagcagc cctggcatca ctaaagggag atatagtgga acttaataaa
      721 cgtctccagc aaacagagag ggaacgggac cttctggaaa agaaattggc caaggcacag
      781 tgcgagcagt cccacctcat gagagagcat gaggatgtcc aggagcgaac gacacttcgc
      841 tatgaggaac gcatcacaga gctccacagc gtcattgcgg agctcaacaa gaagatagac
      901 cgtctgcaag gcaccaccat cagggaggaa gatgagtact cagaactgcg atcagaactc
      961 agccagagcc aacacgaggt caacgaggac tctcgaagca tggaccaaga ccagacctct
     1021 gtctctatcc ccgaaaacca gtctaccatg gttactgctg acatggacaa ctgcagtgac
     1081 ctgaactcag aactgcagag ggtgctgaca gggctggaga atgttgtctg cggcaggaag
     1141 aagagcagct gcagcctctc cgtggccgag gtggacaagc acattgagca gctcaccaca
     1201 gccagcgagc actgtgacct ggctattaag acagtcgagg agattgaggg ggtgcttggc
     1261 cgggacctgt atcccaacct ggctgaagag aggtctcggt gggagaagga gctggctggg
     1321 ctgagggaag agaatgagag cctgactgcc atgctgtgca gcaaagagga agaactgaac
     1381 cggactaagg ccaccatgaa tgccatccgg gaagagcggg accggctccg gaggagggtc
     1441 agagagcttc aaactcgact acagagcgtg caggccacag gtccctccag ccctggccgc
     1501 ctcacttcca ccaaccgccc gattaacccc agcactgggg agctgagcac aagcagcagc
     1561 agcaatgaca ttcccatcgc caagattgct gagagggtga agctatcaaa gacaaggtcc
     1621 gaatcgtcat catctgatcg gccagtcctg ggctcagaaa tcagtagcat aggggtatcc
     1681 agcagtgtgg ctgaacacct ggcccactca cttcaggact gctccaatat ccaagagatt
     1741 ttccaaacac tctactcaca cggatctgcc atctcagaaa gcaagattag agagtttgag
     1801 gtggaaacag aacggctgaa tagccggatt gagcacctca aatcccaaaa tgacctcctg
     1861 accataacct tggaggaatg taaaagcaat gccgagagga tgagcatgct ggtgggaaaa
     1921 tacgaatcca atgccacagc gctgaggctg gccttgcagt acagcgagca gtgcatcgaa
     1981 gcctacgaac tcctcctggc gctggcagag agtgagcaga gcctcatcct ggggcagttc
     2041 cgagcggcgg gcgtggggtc ctcccctgga gaccagtcgg gggatgaaaa catcactcag
     2101 atgctcaagc gagctcatga ctgccggaag acagctgaga acgctgccaa ggccctgctc
     2161 atgaagctgg acggcagctg tgggggagcc tttgccgtgg ccggctgcag cgtgcagccc
     2221 tgggagagcc tttcctccaa cagccacacc agcacaacca gctccacagc cagtagttgc
     2281 gacaccgagt tcactaaaga agacgagcag aggctgaagg attatatcca gcagctcaag
     2341 aatgacaggg ctgcggtcaa gctgaccatg ctggagctgg aaagcatcca catcgatcct
     2401 ctcagctatg acgtcaagcc tcggggagac agccagaggc tggatctgga aaacgcagtg
     2461 cttatgcagg agctcatggc catgaaggag gagatggccg agttgaaggc ccagctctac
     2521 ctactggaga aagagaagaa ggccctggag ctgaagctga gcacgcggga ggcccaggag
     2581 caggcctacc tggtgcacat tgagcacctg aagtccgagg tggaggagca gaaggagcag
     2641 cggatgcgat ccctcagctc caccagcagc ggcagcaaag acaaacctgg caaggagtgt
     2701 gctgatgctg cctccccagc tctgtcccta gccgaactca ggacaacgtg cagcgagaat
     2761 gagctggctg cggagttcac caacgccatt cgtcgagaaa agaagttgaa ggccagagtt
     2821 caagagctgg tgagtgcctt ggagagactc accaagagca gtgaaatccg acatcagcaa
     2881 tctgcagagt tcgtgaatga tctaaagcgg gccaacagca acctggtggc tgcctatgag
     2941 aaagcaaaga aaaagcatca aaacaaactg aagaagttag agtcgcagat gatggccatg
     3001 gtggagagac atgagaccca agtgaggatg ctcaagcaaa gaatagctct gctagaggag
     3061 gagaactcca ggccacacac caatgaaact tcgctttaat cagcactcac gcaccggagt
     3121 tctgcccatg ggaagtaaac tgcagcaggc cactggggac agaagggccc atgtacttgt
     3181 tgggaggagg aggaaaggga aggctggcag gtaggtcggc acttggacaa tggagtgccc
     3241 caactcaacc cttggggcga ctggccatgg tgacattgtg gactgtatcc agaggtgccc
     3301 gctcttccct cctgggccca caacagcgtg taaacacatg ttctgtgcct gctcagcaga
     3361 gcctcgtttc tgctttcagc actcactctc cccctcctct tctggtctgg cggctgtgca
     3421 tcagtgggat cccagacatt tgtttctgta agattttcca ttgtatcctc tttttggtag
     3481 atgctgggct catcttctag aatctcgttt ctcctctttc ctcctgcttc atgggaaaac
     3541 agacctgtgt gtgcctccag catttaaaag gactgctgat ttgtttacta cagcaaggct
     3601 ttggtttcca agtcccgggt ctcaacttta agatagaggc ggccataaga ggtgatctct
     3661 gggagttata ggtcatggga agagcgtaga caggtgttac ttacagtccc agatacacta
     3721 aagttacaaa cagaccacca ccaggactgt gcctgaacaa ttttgtattg agagaataaa
     3781 aacttccttc aatcttcatt ttggaggcag ggctgggaag ggagcgctct cttgattctg
     3841 ggatttctcc ctctcagtgg agccttatta atatccaaga cttagagctg ggaatctttt
     3901 tgatacctgt agtggaacta aaattctgtc aggggtttct tcaagagctg agaaacatta
     3961 ttagcacttc ccgccccagg gcactacata attgctgttc tgctgaatca aatctcttcc
     4021 acatgggtgc atttgtagct ctggacctgt ctctacctaa ggacaagaca ctgaggagat
     4081 actgaacatt ttgcaaaact tatcacgcct acttaagagt gctgtgtaac ccccagttca
     4141 agacttagct cctgttgtca tgacggggac agagtgaggg aatggtagtt aaggcttctt
     4201 ttttgccccc agatacatgg tgatggttag catatggtgc ttaaaaggtt aaatttcaag
     4261 caaaatgctt acagggctag gcagtaccaa agtaactgaa ttatttcagg aaggtcttca
     4321 atcttaaaac aaattcatta ttctttttca gttttacctc ttctctctca gttctacact
     4381 gatacacttg aaggaccatt tactgttttt ttctgtagca ccagagaatc catccaaagt
     4441 tccctatgaa aaatgtgttc cattgccata gctgactaca aattaaagtt gaggaggttt
     4501 ctgcatagag tctttatgtc cataagctac gggtaggtct attttcagag catgatacaa
     4561 attccacagc cttctgttcg ctggaggata ctccagccac cagttcggag ggcagacagc
     4621 tgtatcctaa aagcaaccac tgagaggcca gcagtgaggc tgcccatctc caaaacgaaa
     4681 caacagatca actggtatca gtctagaagg accagctttg gggtgctttg atcactatga
     4741 aaatgttcac tggtttctgt caatcatttg gtcaaagtta gttcctgtga tttttttttt
     4801 ttttttttga gatggagttt tgctcgtcac caggctgaag tgcagtggcg tgatcttggc
     4861 tcactgcaac ctctgcctcc tgagtagctg gaattacagg tgcccgccac catacccagc
     4921 tagttttttt gtatttttag tagagacagg gtttcaccat gttggccagg ctggtctcga
     4981 actcctgacc actgatccac ccacctcggc ctcccaaagt gctgggatta caggcatgag
     5041 ccaatgtgcc tggccattcc tgtgattttt tagaattttt cacttttcat ataaagttgc
     5101 attttatcat gaggaaaatg ttttggctga aaaacatatg ccccaaaaaa aaaaaaatca
     5161 gttttcacta tttttccagc ttgcctaggg aaacgtcctc ccctgagcaa agtgggatgt
     5221 gcatggctct tttttgtgat taagcaggca tcaaatgggc agttttcatt ttcactgaca
     5281 cagaaacatg tggctgaagc aggaataaaa tcctgcctac tgacttctgt ctccattctt
     5341 gctccaggta aaatcagaga acctcccctt ctctgagaat tcccccaggc agaggcggct
     5401 ccagctcctg ttaagggccc cagttttact catgctacct gaccttagtt ttagtacttt
     5461 caaatgtgat ttgggtaaaa actattttgc aggcaaaagt gttaattttt tataaatatg
     5521 tataataaaa aagctataaa agtttttcct tttcttgtat ttttcaaata ctccccaaga
     5581 cacaatcctt gaaggtgaac taggtggcac ttgattatgc taattgtggg agctactgaa
     5641 ctaaactata ctgattaaat taaatgcttg caggttcctg ggtaattttc ctttgtgaat
     5701 aacctggcgc tacctgaatt tttctctaac aaacatctcc aatgagggta aacaatgatc
     5761 ttgattctaa tttatatgag gttaaaaacc ttcagatttc gtattttgaa ttgttctccc
     5821 tagtttcctc agtggttacc cccttttagt cagtaaccat ctctgttcag aattcagtct
     5881 ttggcttttg aagaaatggg cacagagcca aaggacccat ttcacagttc ccttggaata
     5941 tatttcccat tgttggactg aggcaaacaa aaccaactgt gcaaatcaca agcaaaagag
     6001 acttctgtcc gtagttgcga gtgccatcag tgtgagctta ccagctacaa aaatctgtca
     6061 tggactgggg aacagtatga ttccttattg aaactttcat cctcatcggc tggcttgtca
     6121 gcgttctgaa gttaatatcc atgctgcctg aaacaaatgt tatgtttaac tagcacttaa
     6181 cttttaaaat tgcttaattc tcctttcaga tgcatgcact aagtcttctc tgagcctgca
     6241 gcaatatttt agagggacct cttctaagtc agagccttga cattctactc caaaaggaag
     6301 tccaaattag agcccatagg ctttttctta aaagctgtta aggagtagta aagaaagtta
     6361 ccactctaag gaaaacatga aactatctga aagtcttctg gttcctaact ggatacagat
     6421 tagattattt taaatgagaa aaaagtgcac cgtagttacc cgatcaagat tggactgcct
     6481 gtgtccctgg catgaattaa agaacctcca tagttgcttg ttcccttctg aaggtgtttc
     6541 ctatggccca cgagaattat aaagcaatca ctttttaagt ctatcagtca accttaatag
     6601 ctctcaagag tttcgattat caagctttaa agtgataaca tatcaagatg actattaaaa
     6661 aataaacatc tctgaaagtg tcctttcctc cataaggctt taaacaattt gcaaattctt
     6721 aattgggagt atgtcccaat cattctatta aactatttac agctctacct acttctagat
     6781 tcagcaaaca gagctactag ttagagtatt ttgagagcca aaatagtaag agacttctgt
     6841 aatggttgag atttctgcac tgaccttaat tagggtaccg ccatagggct accaaagttt
     6901 ttagtgaggg ctggggaggt ggagcataag taacattttt tttctgcaga aggtgatggc
     6961 tttgtagaac actataaaat ttttcaggaa aacatacagc aaattttctt atccagtggg
     7021 aacagcctat ttgacaagga atgatataca agtttgattc acctttgagc actcttatag
     7081 agtttacaga agaggcgtaa ctggttacaa aacaaaagtt acgccttcac tatccagttc
     7141 ttaaagttta tccacatcct acaaatctta tgttcatgta ggtcaggtta aagtaagctc
     7201 caaacttgtg gccttcagaa gcatctcctg tttaacacac ctgaaattac tctaacttaa
     7261 tacatttgta ttctctaaga aataatgctg actgctgtca ccacagcatc actgatctct
     7321 cagtggctgt taaaatataa acaagtggaa aaaaagtata caagttgata gcagctgttc
     7381 atgtattctt gttttgctaa tacctgtgta gagaaagaca gtttcacatg cagatcacca
     7441 tacatttttg cttaaaggtt acactgggta aactgtttgt ttatcacatt acatctgggt
     7501 gagattttgc atgtgaccaa gaggaagaaa tcattcagtc ccggaaatag caactgctat
     7561 gtaaaaccac atccttgact agtacttcat ctttccacag tcatccatct gctaatcagg
     7621 tgaaatatca gctcagcaga acaaagcaag ttatacacaa atttccccca cattcacact
     7681 gatgccgtac ttagtaattc tggttctacc attccctttt gcactttttg caagtcaaat
     7741 tcatgtggaa aaatgttctg tttgtagaac tcatagtatt cacttatagt gctcatgtca
     7801 ggaatggcaa gtgagtaaga gcccctgata atctgctttt gttagaacgt aatgcaaaga
     7861 taataaaaca tgtccacttc ttttctagtt ctaatcagtt tttagacatc aagatttatt
     7921 tactttgcat gagatttgtt tcctattatt tgaatatgtg ctatacactt gaattagaaa
     7981 aacctgcttg aatgtattgt cagttattaa agcacatgtg actaagtttg catttgtcag
     8041 tattgttcta ctgtttgtac tgaaagtctc attttaaaat tgctacttaa cactgtacta
     8101 taccttgtaa ttttttagat tctccacttg tattctttaa atgtttccag atgcctttag
     8161 ttttagctgc tgtaaaatag ctactttgcg ttatttgata tagaattgtt ttttaaaaac
     8221 tccatttatt tatacagaga catttataat attttacaaa cattcttata ttctgaataa
     8281 atatttctaa ttcca
//
