LOCUS       NM_003791               4377 bp    mRNA    linear   PRI 08-OCT-2024
DEFINITION  Homo sapiens membrane bound transcription factor peptidase, site 1
            (MBTPS1), mRNA.
ACCESSION   NM_003791
VERSION     NM_003791.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4377)
  AUTHORS   Chen,J., Zhou,Y., Liu,Z., Lu,Y., Jiang,Y., Cao,K., Zhou,N.,
            Wang,D., Zhang,C., Zhou,N., Shi,K., Zhang,L., Zhou,L., Wang,Z.,
            Zhang,H., Tang,K., Ma,J., Lv,J. and Huang,B.
  TITLE     Hepatic glycogenesis antagonizes lipogenesis by blocking S1P via
            UDPG
  JOURNAL   Science 383 (6684), eadi3332 (2024)
   PUBMED   38359126
  REMARK    GeneRIF: Hepatic glycogenesis antagonizes lipogenesis by blocking
            S1P via UDPG.
REFERENCE   2  (bases 1 to 4377)
  AUTHORS   Santinelli,R., Benz,N., Guellec,J., Quinquis,F., Kocas,E.,
            Thomas,J., Montier,T., Ka,C., Luczka-Majerus,E., Sage,E., Ferec,C.,
            Coraux,C. and Trouve,P.
  TITLE     The Inhibition of the Membrane-Bound Transcription Factor Site-1
            Protease (MBTP1) Alleviates the p.Phe508del-Cystic Fibrosis
            Transmembrane Conductance Regulator (CFTR) Defects in Cystic
            Fibrosis Cells
  JOURNAL   Cells 13 (2), 185 (2024)
   PUBMED   38247876
  REMARK    GeneRIF: The Inhibition of the Membrane-Bound Transcription Factor
            Site-1 Protease (MBTP1) Alleviates the p.Phe508del-Cystic Fibrosis
            Transmembrane Conductance Regulator (CFTR) Defects in Cystic
            Fibrosis Cells.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 4377)
  AUTHORS   Liu,X.T., Huang,Y., Liu,D., Jiang,Y.C., Zhao,M., Chung,L.H.,
            Han,X.D., Zhao,Y., Chen,J., Coleman,P., Ting,K.K., Tran,C., Su,Y.,
            Dennis,C.V., Bhatnagar,A., Liu,K., Don,A.S., Vadas,M.A.,
            Gorrell,M.D., Zhang,S., Murray,M., Kavurma,M.M., McCaughan,G.W.,
            Gamble,J.R. and Qi,Y.
  TITLE     Targeting the SphK1/S1P/PFKFB3 axis suppresses hepatocellular
            carcinoma progression by disrupting glycolytic energy supply that
            drives tumor angiogenesis
  JOURNAL   J Transl Med 22 (1), 43 (2024)
   PUBMED   38200582
  REMARK    GeneRIF: Targeting the SphK1/S1P/PFKFB3 axis suppresses
            hepatocellular carcinoma progression by disrupting glycolytic
            energy supply that drives tumor angiogenesis.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 4377)
  AUTHORS   Fang,C., Ren,P., Bian,G., Wang,J., Bai,J., Huang,J., Ding,Y.,
            Li,X., Li,M. and Hou,Z.
  TITLE     Enhancing Spns2/S1P in macrophages alleviates hyperinflammation and
            prevents immunosuppression in sepsis
  JOURNAL   EMBO Rep 24 (8), e56635 (2023)
   PUBMED   37358015
  REMARK    GeneRIF: Enhancing Spns2/S1P in macrophages alleviates
            hyperinflammation and prevents immunosuppression in sepsis.
REFERENCE   5  (bases 1 to 4377)
  AUTHORS   Li,F., Zhang,Y., Lin,Z., Yan,L., Liu,Q., Li,Y., Pei,X., Feng,Y.,
            Han,X., Yang,J., Zheng,F., Li,T., Zhang,Y., Fu,Z., Shao,D., Yu,J.
            and Li,C.
  TITLE     Targeting SPHK1/S1PR3-regulated S-1-P metabolic disorder triggers
            autophagic cell death in pulmonary lymphangiomyomatosis (LAM)
  JOURNAL   Cell Death Dis 13 (12), 1065 (2022)
   PUBMED   36543771
  REMARK    GeneRIF: Targeting SPHK1/S1PR3-regulated S-1-P metabolic disorder
            triggers autophagic cell death in pulmonary lymphangiomyomatosis
            (LAM).
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 4377)
  AUTHORS   Seidah,N.G., Mowla,S.J., Hamelin,J., Mamarbachi,A.M., Benjannet,S.,
            Toure,B.B., Basak,A., Munzer,J.S., Marcinkiewicz,J., Zhong,M.,
            Barale,J.C., Lazure,C., Murphy,R.A., Chretien,M. and
            Marcinkiewicz,M.
  TITLE     Mammalian subtilisin/kexin isozyme SKI-1: A widely expressed
            proprotein convertase with a unique cleavage specificity and
            cellular localization
  JOURNAL   Proc Natl Acad Sci U S A 96 (4), 1321-1326 (1999)
   PUBMED   9990022
REFERENCE   7  (bases 1 to 4377)
  AUTHORS   Sakai,J., Rawson,R.B., Espenshade,P.J., Cheng,D., Seegmiller,A.C.,
            Goldstein,J.L. and Brown,M.S.
  TITLE     Molecular identification of the sterol-regulated luminal protease
            that cleaves SREBPs and controls lipid composition of animal cells
  JOURNAL   Mol Cell 2 (4), 505-514 (1998)
   PUBMED   9809072
REFERENCE   8  (bases 1 to 4377)
  AUTHORS   Duncan,E.A., Brown,M.S., Goldstein,J.L. and Sakai,J.
  TITLE     Cleavage site for sterol-regulated protease localized to a leu-Ser
            bond in the lumenal loop of sterol regulatory element-binding
            protein-2
  JOURNAL   J Biol Chem 272 (19), 12778-12785 (1997)
   PUBMED   9139737
REFERENCE   9  (bases 1 to 4377)
  AUTHORS   Siezen,R.J. and Leunissen,J.A.
  TITLE     Subtilases: the superfamily of subtilisin-like serine proteases
  JOURNAL   Protein Sci 6 (3), 501-523 (1997)
   PUBMED   9070434
  REMARK    Review article
REFERENCE   10 (bases 1 to 4377)
  AUTHORS   Wang,H., Wierenga,A., Prabhu,S. and Wierenga,K.
  TITLE     MBTPS1-Related Spondyloepimetaphyseal Dysplasia with Elevated
            Lysosomal Enzymes
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   38048414
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BP364386.1, D42053.1,
            BC114555.1 and BM680126.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_003791.3.
            
            Summary: This gene encodes a member of the subtilisin-like
            proprotein convertase family, which includes proteases that process
            protein and peptide precursors trafficking through regulated or
            constitutive branches of the secretory pathway. The encoded protein
            undergoes an initial autocatalytic processing event in the ER to
            generate a heterodimer which exits the ER and sorts to the
            cis/medial-Golgi where a second autocatalytic event takes place and
            the catalytic activity is acquired. It encodes a type 1 membrane
            bound protease which is ubiquitously expressed and regulates
            cholesterol or lipid homeostasis via cleavage of substrates at
            non-basic residues. Mutations in this gene may be associated with
            lysosomal dysfunction. [provided by RefSeq, Feb 2014].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: D42053.1, BC114555.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000343411.8/ ENSP00000344223.3
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-36                BP364386.1         2-37
            37-113              D42053.1           1-77
            114-3854            BC114555.1         1-3741
            3855-4377           BM680126.1         16-538              c
FEATURES             Location/Qualifiers
     source          1..4377
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="16"
                     /map="16q23.3-q24.1"
     gene            1..4377
                     /gene="MBTPS1"
                     /gene_synonym="PCSK8; S1P; SEDKF; SKI-1"
                     /note="membrane bound transcription factor peptidase, site
                     1"
                     /db_xref="GeneID:8720"
                     /db_xref="HGNC:HGNC:15456"
                     /db_xref="MIM:603355"
     exon            1..208
                     /gene="MBTPS1"
                     /gene_synonym="PCSK8; S1P; SEDKF; SKI-1"
                     /inference="alignment:Splign:2.1.0"
     exon            209..695
                     /gene="MBTPS1"
                     /gene_synonym="PCSK8; S1P; SEDKF; SKI-1"
                     /inference="alignment:Splign:2.1.0"
     CDS             533..3691
                     /gene="MBTPS1"
                     /gene_synonym="PCSK8; S1P; SEDKF; SKI-1"
                     /EC_number="3.4.21.112"
                     /note="site-1 protease; subtilisin/kexin isozyme-1;
                     membrane-bound transcription factor site-1 protease;
                     endopeptidase S1P; proprotein convertase subtilisin/kexin
                     type 8"
                     /codon_start=1
                     /product="membrane-bound transcription factor site-1
                     protease preproprotein"
                     /protein_id="NP_003782.1"
                     /db_xref="CCDS:CCDS10941.1"
                     /db_xref="GeneID:8720"
                     /db_xref="HGNC:HGNC:15456"
                     /db_xref="MIM:603355"
                     /translation="MKLVNIWLLLLVVLLCGKKHLGDRLEKKSFEKAPCPGCSHLTLK
                     VEFSSTVVEYEYIVAFNGYFTAKARNSFISSALKSSEVDNWRIIPRNNPSSDYPSDFE
                     VIQIKEKQKAGLLTLEDHPNIKRVTPQRKVFRSLKYAESDPTVPCNETRWSQKWQSSR
                     PLRRASLSLGSGFWHATGRHSSRRLLRAIPRQVAQTLQADVLWQMGYTGANVRVAVFD
                     TGLSEKHPHFKNVKERTNWTNERTLDDGLGHGTFVAGVIASMRECQGFAPDAELHIFR
                     VFTNNQVSYTSWFLDAFNYAILKKIDVLNLSIGGPDFMDHPFVDKVWELTANNVIMVS
                     AIGNDGPLYGTLNNPADQMDVIGVGGIDFEDNIARFSSRGMTTWELPGGYGRMKPDIV
                     TYGAGVRGSGVKGGCRALSGTSVASPVVAGAVTLLVSTVQKRELVNPASMKQALIASA
                     RRLPGVNMFEQGHGKLDLLRAYQILNSYKPQASLSPSYIDLTECPYMWPYCSQPIYYG
                     GMPTVVNVTILNGMGVTGRIVDKPDWQPYLPQNGDNIEVAFSYSSVLWPWSGYLAISI
                     SVTKKAASWEGIAQGHVMITVASPAETESKNGAEQTSTVKLPIKVKIIPTPPRSKRVL
                     WDQYHNLRYPPGYFPRDNLRMKNDPLDWNGDHIHTNFRDMYQHLRSMGYFVEVLGAPF
                     TCFDASQYGTLLMVDSEEEYFPEEIAKLRRDVDNGLSLVIFSDWYNTSVMRKVKFYDE
                     NTRQWWMPDTGGANIPALNELLSVWNMGFSDGLYEGEFTLANHDMYYASGCSIAKFPE
                     DGVVITQTFKDQGLEVLKQETAVVENVPILGLYQIPAEGGGRIVLYGDSNCLDDSHRQ
                     KDCFWLLDALLQYTSYGVTPPSLSHSGNRQRPPSGAGSVTPERMEGNHLHRYSKVLEA
                     HLGDPKPRPLPACPRLSWAKPQPLNETAPSNLWKHQKLLSIDLDKVVLPNFRSNRPQV
                     RPLSPGESGAWDIPGGIMPGRYNQEVGQTIPVFAFLGAMVVLAFFVVQINKAKSRPKR
                     RKPRVKRPQLMQQVHPPKTPSV"
     exon            696..953
                     /gene="MBTPS1"
                     /gene_synonym="PCSK8; S1P; SEDKF; SKI-1"
                     /inference="alignment:Splign:2.1.0"
     exon            954..1157
                     /gene="MBTPS1"
                     /gene_synonym="PCSK8; S1P; SEDKF; SKI-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1158..1268
                     /gene="MBTPS1"
                     /gene_synonym="PCSK8; S1P; SEDKF; SKI-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1269..1378
                     /gene="MBTPS1"
                     /gene_synonym="PCSK8; S1P; SEDKF; SKI-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1379..1495
                     /gene="MBTPS1"
                     /gene_synonym="PCSK8; S1P; SEDKF; SKI-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1496..1563
                     /gene="MBTPS1"
                     /gene_synonym="PCSK8; S1P; SEDKF; SKI-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1564..1666
                     /gene="MBTPS1"
                     /gene_synonym="PCSK8; S1P; SEDKF; SKI-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1667..1818
                     /gene="MBTPS1"
                     /gene_synonym="PCSK8; S1P; SEDKF; SKI-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1819..1980
                     /gene="MBTPS1"
                     /gene_synonym="PCSK8; S1P; SEDKF; SKI-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1981..2125
                     /gene="MBTPS1"
                     /gene_synonym="PCSK8; S1P; SEDKF; SKI-1"
                     /inference="alignment:Splign:2.1.0"
     exon            2126..2314
                     /gene="MBTPS1"
                     /gene_synonym="PCSK8; S1P; SEDKF; SKI-1"
                     /inference="alignment:Splign:2.1.0"
     exon            2315..2487
                     /gene="MBTPS1"
                     /gene_synonym="PCSK8; S1P; SEDKF; SKI-1"
                     /inference="alignment:Splign:2.1.0"
     exon            2488..2603
                     /gene="MBTPS1"
                     /gene_synonym="PCSK8; S1P; SEDKF; SKI-1"
                     /inference="alignment:Splign:2.1.0"
     exon            2604..2760
                     /gene="MBTPS1"
                     /gene_synonym="PCSK8; S1P; SEDKF; SKI-1"
                     /inference="alignment:Splign:2.1.0"
     exon            2761..2885
                     /gene="MBTPS1"
                     /gene_synonym="PCSK8; S1P; SEDKF; SKI-1"
                     /inference="alignment:Splign:2.1.0"
     exon            2886..2963
                     /gene="MBTPS1"
                     /gene_synonym="PCSK8; S1P; SEDKF; SKI-1"
                     /inference="alignment:Splign:2.1.0"
     exon            2964..3104
                     /gene="MBTPS1"
                     /gene_synonym="PCSK8; S1P; SEDKF; SKI-1"
                     /inference="alignment:Splign:2.1.0"
     exon            3105..3236
                     /gene="MBTPS1"
                     /gene_synonym="PCSK8; S1P; SEDKF; SKI-1"
                     /inference="alignment:Splign:2.1.0"
     exon            3237..3363
                     /gene="MBTPS1"
                     /gene_synonym="PCSK8; S1P; SEDKF; SKI-1"
                     /inference="alignment:Splign:2.1.0"
     exon            3364..3494
                     /gene="MBTPS1"
                     /gene_synonym="PCSK8; S1P; SEDKF; SKI-1"
                     /inference="alignment:Splign:2.1.0"
     exon            3495..4377
                     /gene="MBTPS1"
                     /gene_synonym="PCSK8; S1P; SEDKF; SKI-1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4346..4351
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MBTPS1"
                     /gene_synonym="PCSK8; S1P; SEDKF; SKI-1"
                     /note="hexamer: AATAAA"
     polyA_site      4377
                     /gene="MBTPS1"
                     /gene_synonym="PCSK8; S1P; SEDKF; SKI-1"
                     /note="major polyA site"
ORIGIN      
        1 agagcgcgca cgcaagcccc gggagcgcgg gacagacagg gcacgctggg tcggcggagc
       61 tgaggctccc agctgtgggc ctcgctggcc cggtcgccca gtctcgcgag agttgggagt
      121 aaacagcccc gaatggagtg cccaggcgtg ttcgccgcgg aggcgccgtt atcccgggcc
      181 cgccggccct gagctcccgg cggcgcagat tggctcacag tggttgattg atcaacccca
      241 ttggacgttg gttctgtggt acaaatggag tacaggactc agtcgtcacg gcctgagtga
      301 gagaagcctt atttccaaga tggagaagaa gcggagaaag aaatgaaagc ctctcttcag
      361 gctgaaccac aaaaggccat gggatttaac ttttatttat gttgggcaag actgtaagat
      421 ggctgattag taatgttgca gcttttagct gaaacaaaaa ttcactttta atcaagaaga
      481 aaaaagtgtg atttgaatat atgcaatttt atgatcatat tcgcttgtga ccatgaagct
      541 tgtcaacatc tggctgcttc tgctcgtggt tttgctctgt gggaagaaac atctgggcga
      601 cagactggaa aagaaatctt ttgaaaaggc cccatgccct ggctgttccc acctgacttt
      661 gaaggtggaa ttctcatcaa cagttgtgga atatgaatat attgtggctt tcaatggata
      721 ctttacagcc aaagctagaa attcatttat ttcaagtgcc ctgaagagca gtgaagtaga
      781 caattggaga attatacctc gaaacaatcc atccagtgac taccctagtg attttgaggt
      841 gattcagata aaagaaaaac agaaagcggg gctgctaaca cttgaagatc atccaaacat
      901 caaacgggtc acgccccaac gaaaagtctt tcgttccctc aagtatgctg aatctgaccc
      961 cacagtaccc tgcaatgaaa cccggtggag ccagaagtgg caatcatcac gtcccctgcg
     1021 aagagccagc ctctccctgg gctctggctt ctggcatgct acgggaaggc attcgagcag
     1081 acggctgctg agagccatcc cgcgccaggt tgcccagaca ctgcaggcag atgtgctctg
     1141 gcagatggga tatacaggtg ctaatgtaag agttgctgtt tttgacactg ggctgagcga
     1201 gaagcatccc cacttcaaaa atgtgaagga gagaaccaac tggaccaacg agcgaacgct
     1261 ggacgatggg ttgggccatg gcacattcgt ggcaggtgtg atagccagca tgagggagtg
     1321 ccaaggattt gctccagatg cagaacttca cattttcagg gtctttacca ataatcaggt
     1381 atcttacaca tcttggtttt tggacgcctt caactatgcc attttaaaga agatcgacgt
     1441 gttaaacctc agcatcggcg gcccggactt catggatcat ccgtttgttg acaaggtgtg
     1501 ggaattaaca gctaacaatg taatcatggt ttctgctatt ggcaatgacg gacctcttta
     1561 tggcactctg aataaccctg ctgatcaaat ggatgtgatt ggagtaggcg gcattgactt
     1621 tgaagataac atcgcccgct tttcttcaag gggaatgact acctgggagc taccaggagg
     1681 ctacggtcgc atgaaacctg acattgtcac ctatggtgct ggcgtgcggg gttctggcgt
     1741 gaaagggggg tgccgggccc tctcagggac cagtgttgct tctccagtgg ttgcaggtgc
     1801 tgtcaccttg ttagtgagca cagtccagaa gcgtgagctg gtgaatcccg ccagtatgaa
     1861 gcaggccctg atcgcgtcag cccggaggct ccccggggtc aacatgtttg agcaaggcca
     1921 cggcaagctc gatctgctca gagcctatca gatcctcaac agctacaagc cacaggcaag
     1981 tttgagcccc agctacatag atctgactga gtgtccctac atgtggccct actgctccca
     2041 gcccatctac tatggaggaa tgccgacagt tgttaatgtc accatcctca acggcatggg
     2101 agtcacagga agaattgtag ataagcctga ctggcagccc tatttgccac agaacggaga
     2161 caacattgaa gttgccttct cctactcctc ggtcttatgg ccttggtcgg gctacctggc
     2221 catctccatt tctgtgacca agaaagcggc ttcctgggaa ggcattgctc agggccatgt
     2281 catgatcact gtggcttccc cagcagagac agagtcaaaa aatggtgcag aacagacttc
     2341 aacagtaaag ctccccatta aggtgaagat aattcctact cccccgcgaa gcaagagagt
     2401 tctctgggat cagtaccaca acctccgcta tccacctggc tatttcccca gggataattt
     2461 aaggatgaag aatgaccctt tagactggaa tggtgatcac atccacacca atttcaggga
     2521 tatgtaccag catctgagaa gcatgggcta ctttgtagag gtcctcgggg cccccttcac
     2581 gtgttttgat gccagtcagt atggcacttt gctgatggtg gacagtgagg aggagtactt
     2641 ccctgaagag atcgccaagc tccggaggga cgtggacaac ggcctctcgc tcgtcatctt
     2701 cagtgactgg tacaacactt ctgttatgag aaaagtgaag ttttatgatg aaaacacaag
     2761 gcagtggtgg atgccggata ccggaggagc taacatccca gctctgaatg agctgctgtc
     2821 tgtgtggaac atggggttca gcgatggcct gtatgaaggg gagttcaccc tggccaacca
     2881 tgacatgtat tatgcgtcag ggtgcagcat cgcgaagttt ccagaagatg gcgtcgtgat
     2941 aacacagact ttcaaggacc aaggattgga ggttttaaag caggaaacag cagttgttga
     3001 aaacgtcccc attttgggac tttatcagat tccagctgag ggtggaggcc ggattgtact
     3061 gtatggggac tccaattgct tggatgacag tcaccgacag aaggactgct tttggcttct
     3121 ggatgccctc ctccagtaca catcgtatgg ggtgacaccg cctagcctca gtcactctgg
     3181 gaaccgccag cgccctccca gtggagcagg ctcagtcact ccagagagga tggaaggaaa
     3241 ccatcttcat cggtactcca aggttctgga ggcccatttg ggagacccaa aacctcggcc
     3301 tctaccagcc tgtccacgct tgtcttgggc caagccacag cctttaaacg agacggcgcc
     3361 cagtaacctt tggaaacatc agaagctact ctccattgac ctggacaagg tggtgttacc
     3421 caactttcga tcgaatcgcc ctcaagtgag gcccttgtcc cctggagaga gcggcgcctg
     3481 ggacattcct ggagggatca tgcctggccg ctacaaccag gaggtgggcc agaccattcc
     3541 tgtctttgcc ttcctgggag ccatggtggt cctggccttc tttgtggtac aaatcaacaa
     3601 ggccaagagc aggccgaagc ggaggaagcc cagggtgaag cgcccgcagc tcatgcagca
     3661 ggttcacccg ccaaagaccc cttcggtgtg accggcagcc tggctgaccg tgagggccag
     3721 agagagcctt cacggacggc gctggtgggt gagccgagct gtggtggcgg ctggtttaaa
     3781 agggatccag tttccagctg caggtttgtt agagtctgtt ctacatgggc ctgccctcct
     3841 gtgatgggca gaggctcctg gtacatcgag aagattcctg tggatcccgt caggagggac
     3901 ttagtggctc tgccgccagt gagacttccc gccggcagct gtgcgcacca aagactcggg
     3961 agaactggaa aggctgtctg gggtcttctg actgcagggg aaggatgtac tttccaaaca
     4021 aatgatacaa ccctgaccaa gctaaaagac gcttgttaaa ggctattttc tatatttatt
     4081 gttgggaaaa gtcactttaa agacttgtgc tatttggaag caaagctatt ttttttgtca
     4141 gtggaatgca gtttttttac tattccatca tgaggaacaa catagattcc atgatctttt
     4201 taatgacagt acagactgag atttgaagga aacatgcaca aatctgtaaa acatagacct
     4261 tcgctttatt tttgtaagta tcacctgcca ccatgttttg taatttgagg tcttgatttc
     4321 accattgtcg gtgaagaaaa ttttcaataa atatgtatta cccgtctgaa gcttaaa
//
