LOCUS       NM_001100916            1644 bp    mRNA    linear   PRI 02-FEB-2025
DEFINITION  Homo sapiens membrane bound ghrelin O-acyltransferase MBOAT4
            (MBOAT4), mRNA.
ACCESSION   NM_001100916 XM_001125855 XM_001717299 XM_940502
VERSION     NM_001100916.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1644)
  AUTHORS   Holliday,A., Warner,J., Hulston,C.J., Corfe,B.M. and Crabtree,D.R.
  TITLE     Anorexia of ageing is associated with elevated fasted and lower
            post-prandial ghrelin, independent of ghrelin O-acyltransferase
  JOURNAL   Appetite 196, 107259 (2024)
   PUBMED   38341037
  REMARK    GeneRIF: Anorexia of ageing is associated with elevated fasted and
            lower post-prandial ghrelin, independent of ghrelin
            O-acyltransferase.
REFERENCE   2  (bases 1 to 1644)
  AUTHORS   Hornsby,A.K.E., Buntwal,L., Carisi,M.C., Santos,V.V., Johnston,F.,
            Roberts,L.D., Sassi,M., Mequinion,M., Stark,R., Reichenbach,A.,
            Lockie,S.H., Siervo,M., Howell,O., Morgan,A.H., Wells,T.,
            Andrews,Z.B., Burn,D.J. and Davies,J.S.
  TITLE     Unacylated-Ghrelin Impairs Hippocampal Neurogenesis and Memory in
            Mice and Is Altered in Parkinson's Dementia in Humans
  JOURNAL   Cell Rep Med 1 (7), 100120 (2020)
   PUBMED   33103129
  REMARK    GeneRIF: Unacylated-Ghrelin Impairs Hippocampal Neurogenesis and
            Memory in Mice and Is Altered in Parkinson's Dementia in Humans.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 1644)
  AUTHORS   Nakata,S., Yoshino,Y., Okita,M., Kawabe,K., Yamazaki,K., Ozaki,Y.,
            Mori,Y., Ochi,S., Iga,J.I. and Ueno,S.I.
  TITLE     Differential expression of the ghrelin-related mRNAs GHS-R1a,
            GHS-R1b, and MBOAT4 in Japanese patients with schizophrenia
  JOURNAL   Psychiatry Res 272, 334-339 (2019)
   PUBMED   30597386
  REMARK    GeneRIF: MBOAT4 expression is significantly increased in Japanese
            patients with schizophrenia compared to controls.
REFERENCE   4  (bases 1 to 1644)
  AUTHORS   Gomez-Gomez,E., Jimenez-Vacas,J.M., Carrasco-Valiente,J.,
            Herrero-Aguayo,V., Blanca-Pedregosa,A.M., Leon-Gonzalez,A.J.,
            Valero-Rosa,J., Fernandez-Rueda,J.L., Gonzalez-Serrano,T.,
            Lopez-Miranda,J., Gahete,M.D., Castano,J.P., Requena-Tapia,M.J. and
            Luque,R.M.
  TITLE     Plasma ghrelin O-acyltransferase (GOAT) enzyme levels: A novel
            non-invasive diagnosis tool for patients with significant prostate
            cancer
  JOURNAL   J Cell Mol Med 22 (11), 5688-5697 (2018)
   PUBMED   30256519
  REMARK    GeneRIF: GOAT-levels could also represent a novel predictive
            biomarker of aggressiveness, as its levels are positively
            associated with Gleason Score and the presence of metastasis at the
            time of diagnoses.
REFERENCE   5  (bases 1 to 1644)
  AUTHORS   Herrera-Martinez,A.D., Gahete,M.D., Sanchez-Sanchez,R.,
            Alors-Perez,E., Pedraza-Arevalo,S., Serrano-Blanch,R.,
            Martinez-Fuentes,A.J., Galvez-Moreno,M.A., Castano,J.P. and
            Luque,R.M.
  TITLE     Ghrelin-O-Acyltransferase (GOAT) Enzyme as a Novel Potential
            Biomarker in Gastroenteropancreatic Neuroendocrine Tumors
  JOURNAL   Clin Transl Gastroenterol 9 (10), 196 (2018)
   PUBMED   30297816
  REMARK    GeneRIF: GOAT was significantly overexpressed in
            gastroenteropancreatic tumor samples compared to adjacent non-tumor
            tissue
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 1644)
  AUTHORS   Muller,T.D., Tschop,M.H., Jarick,I., Ehrlich,S., Scherag,S.,
            Herpertz-Dahlmann,B., Zipfel,S., Herzog,W., de Zwaan,M.,
            Burghardt,R., Fleischhaker,C., Klampfl,K., Wewetzer,C.,
            Herpertz,S., Zeeck,A., Tagay,S., Burgmer,M., Pfluger,P.T.,
            Scherag,A., Hebebrand,J. and Hinney,A.
  TITLE     Genetic variation of the ghrelin activator gene ghrelin
            O-acyltransferase (GOAT) is associated with anorexia nervosa
  JOURNAL   J Psychiatr Res 45 (5), 706-711 (2011)
   PUBMED   21035823
  REMARK    GeneRIF: Based on the results of tjhis study concluded that genetic
            variation in GOAT might be implicated in the etiology of anorexia
            nervosa.
            GeneRIF: Observational study of gene-disease association. (HuGE
            Navigator)
REFERENCE   7  (bases 1 to 1644)
  AUTHORS   Heppner,K.M., Tong,J., Kirchner,H., Nass,R. and Tschop,M.H.
  TITLE     The ghrelin O-acyltransferase-ghrelin system: a novel regulator of
            glucose metabolism
  JOURNAL   Curr Opin Endocrinol Diabetes Obes 18 (1), 50-55 (2011)
   PUBMED   21150588
  REMARK    GeneRIF: Modulation of GOAT or ghrelin signaling may be a
            clinically relevant strategy to treat metabolic diseases such as
            type II diabetes.
            Review article
REFERENCE   8  (bases 1 to 1644)
  AUTHORS   Takahashi,T., Ida,T., Sato,T., Nakashima,Y., Nakamura,Y., Tsuji,A.
            and Kojima,M.
  TITLE     Production of n-octanoyl-modified ghrelin in cultured cells
            requires prohormone processing protease and ghrelin
            O-acyltransferase, as well as n-octanoic acid
  JOURNAL   J Biochem 146 (5), 675-682 (2009)
   PUBMED   19628676
  REMARK    GeneRIF: The presence of PC and GOAT in the cells, as well as
            n-octanoic acid in the culture medium, was necessary to produce
            n-octanoyl ghrelin.
REFERENCE   9  (bases 1 to 1644)
  AUTHORS   Gomez,R., Lago,F., Gomez-Reino,J.J., Dieguez,C. and Gualillo,O.
  TITLE     Expression and modulation of ghrelin O-acyltransferase in cultured
            chondrocytes
  JOURNAL   Arthritis Rheum 60 (6), 1704-1709 (2009)
   PUBMED   19479831
  REMARK    GeneRIF: GOAT was expressed in human immortalized chondrocyte cell
            lines and in human cultured primary chondrocytes.
REFERENCE   10 (bases 1 to 1644)
  AUTHORS   Gutierrez,J.A., Solenberg,P.J., Perkins,D.R., Willency,J.A.,
            Knierman,M.D., Jin,Z., Witcher,D.R., Luo,S., Onyia,J.E. and
            Hale,J.E.
  TITLE     Ghrelin octanoylation mediated by an orphan lipid transferase
  JOURNAL   Proc Natl Acad Sci U S A 105 (17), 6320-6325 (2008)
   PUBMED   18443287
  REMARK    GeneRIF: The identification and characterization of human GOAT, the
            ghrelin O-acyl transferase, is reported.
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            EU518495.2 and AC026979.9.
            
            On May 17, 2019 this sequence version replaced NM_001100916.1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The extent of this transcript is
            supported by transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: EU518495.2, DN930415.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2142586, SAMEA2146982
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000320542.4/ ENSP00000314196.3
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-199               EU518495.2         30-228
            200-298             AC026979.9         32427-32525         c
            299-1266            EU518495.2         328-1295
            1267-1644           AC026979.9         25456-25833         c
FEATURES             Location/Qualifiers
     source          1..1644
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="8"
                     /map="8p12"
     gene            1..1644
                     /gene="MBOAT4"
                     /gene_synonym="FKSG89; GOAT; OACT4"
                     /note="membrane bound ghrelin O-acyltransferase MBOAT4"
                     /db_xref="GeneID:619373"
                     /db_xref="HGNC:HGNC:32311"
                     /db_xref="MIM:611940"
     exon            1..183
                     /gene="MBOAT4"
                     /gene_synonym="FKSG89; GOAT; OACT4"
                     /inference="alignment:Splign:2.1.0"
     CDS             65..1372
                     /gene="MBOAT4"
                     /gene_synonym="FKSG89; GOAT; OACT4"
                     /EC_number="2.3.1.-"
                     /note="O-acyltransferase (membrane bound) domain
                     containing 4; O-acyltransferase domain-containing protein
                     4; membrane-bound O-acyltransferase domain-containing
                     protein 4; membrane bound O-acyltransferase domain
                     containing 4"
                     /codon_start=1
                     /product="ghrelin O-acyltransferase"
                     /protein_id="NP_001094386.1"
                     /db_xref="CCDS:CCDS47835.1"
                     /db_xref="GeneID:619373"
                     /db_xref="HGNC:HGNC:32311"
                     /db_xref="MIM:611940"
                     /translation="MEWLWLFFLHPISFYQGAAFPFALLFNYLCIMDSFSTRARYLFL
                     LTGGGALAVAAMGSYAVLVFTPAVCAVALLCSLAPQQVHRWTFCFQMSWQTLCHLGLH
                     YTEYYLHEPPSVRFCITLSSLMLLTQRVTSLSLDICEGKVKAASGGFRSRSSLSEHVC
                     KALPYFSYLLFFPALLGGSLCSFQRFQARVQGSSALHPRHSFWALSWRGLQILGLECL
                     NVAVSRVVDAGAGLTDCQQFECIYVVWTTAGLFKLTYYSHWILDDSLLHAAGFGPELG
                     QSPGEEGYVPDADIWTLERTHRISVFSRKWNQSTARWLRRLVFQHSRAWPLLQTFAFS
                     AWWHGLHPGQVFGFVCWAVMVEADYLIHSFANEFIRSWPMRLFYRTLTWAHTQLIIAY
                     IMLAVEVRSLSSLWLLCNSYNSVFPMVYCILLLLLAKRKHKCN"
     exon            184..408
                     /gene="MBOAT4"
                     /gene_synonym="FKSG89; GOAT; OACT4"
                     /inference="alignment:Splign:2.1.0"
     exon            409..1644
                     /gene="MBOAT4"
                     /gene_synonym="FKSG89; GOAT; OACT4"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1466..1471
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MBOAT4"
                     /gene_synonym="FKSG89; GOAT; OACT4"
                     /note="hexamer: AATAAA"
     polyA_site      1499
                     /gene="MBOAT4"
                     /gene_synonym="FKSG89; GOAT; OACT4"
                     /note="major polyA site"
ORIGIN      
        1 actcttggag gaaggcatca gttaaggaca ctggaaaaga caggctcttt tgttctggtt
       61 cctaatggag tggctttggc tgttctttct ccatcctata tcgttttacc agggggctgc
      121 atttcccttt gcacttctct tcaattatct ctgcatcatg gattcattct ccactcgtgc
      181 caggtacctc tttctcctga ctggaggagg tgccctggcc gtggctgcca tgggttccta
      241 cgccgtgctc gtcttcaccc ctgctgtctg cgctgtggct ctcctctgtt ccctggctcc
      301 tcagcaagtc cacaggtgga ccttctgctt tcagatgagc tggcagacct tgtgtcacct
      361 aggtctgcac tacactgagt attatctgca tgagcctcct tctgtgaggt tctgcatcac
      421 tctttcttct ctcatgctct tgacccagag ggtcacgtcc ctctctctgg acatttgtga
      481 ggggaaagtg aaggcagcat ctggaggctt caggagcagg agctctttgt ctgagcatgt
      541 gtgtaaggca ctgccctatt tcagctactt gctctttttc cctgctctcc tgggaggctc
      601 tctgtgctcc ttccagcgat ttcaggctcg tgttcaaggg tccagtgctt tgcatcccag
      661 acactctttc tgggctctga gctggagggg tctgcagatt cttggactag aatgcctaaa
      721 cgtggcagtg agcagggtgg tggatgcagg agcgggactg actgattgcc agcaattcga
      781 gtgcatctat gtcgtgtgga ccacagctgg gcttttcaag ctcacctact actcccactg
      841 gatcctggac gactccctcc tccacgcagc gggctttggg cctgagcttg gtcagagccc
      901 tggagaggag ggatatgtcc ccgatgcaga catctggacc ctggaaagaa cccacaggat
      961 atctgtgttc tcaagaaagt ggaaccaaag cacagctcga tggctccgac ggcttgtatt
     1021 ccagcacagc agggcttggc cgttgttgca gacatttgcc ttctctgcct ggtggcatgg
     1081 actccatcca ggacaggtgt ttggtttcgt ttgctgggcc gtgatggtgg aagctgacta
     1141 cctgattcac tcctttgcca atgagtttat cagatcctgg ccgatgaggc tgttctatag
     1201 aaccctcacc tgggcccaca cccagttgat cattgcctac atcatgctgg ctgtggaggt
     1261 caggagtctc tcctctctct ggttgctctg taattcgtac aacagtgtct ttcccatggt
     1321 gtactgtatt ctgcttttgc tattggcgaa gagaaagcac aaatgtaact gacatctttc
     1381 cctggctttc accttaaaca cccaggaaac attttagaaa gtgccagatg acgcatcgat
     1441 aatgcataca tttgaacttt tccataataa aattttttct aaagttttgg atggatatac
     1501 ccagtgactt gcagtaggaa tttgtgtatc tactttagca agctattctg ctggtactga
     1561 ggatacaaaa aggaaaaaga tgtggtttct tcccttaagg tttacagtct agatgtggaa
     1621 gcaaagatta agtaaagtga taca
//
