LOCUS       NM_001378120           10732 bp    mRNA    linear   PRI 12-OCT-2024
DEFINITION  Homo sapiens methyl-CpG binding domain protein 5 (MBD5), transcript
            variant 1, mRNA.
ACCESSION   NM_001378120 XM_024452987
VERSION     NM_001378120.1
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 10732)
  AUTHORS   Bhatia,M., Cavalleri,G.L., White,M., Delanty,N., Sweeney,B.J.,
            Costello,D.J., Greally,M.T. and Benson,K.A.
  TITLE     Germline mosaicism in a family with MBD5 haploinsufficiency
  JOURNAL   Cold Spring Harb Mol Case Stud 8 (7), a006253 (2022)
   PUBMED   36396431
  REMARK    GeneRIF: Germline mosaicism in a family with MBD5
            haploinsufficiency.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10732)
  AUTHORS   Jing,X.W., Cheng,M.M., Niu,X.Y., Yang,Y., Yang,X.L., Yang,Z.X. and
            Zhang,Y.H.
  TITLE     [Clinical phenotypes and genetic features of epilepsy children with
            MBD5 gene variants]
  JOURNAL   Zhonghua Er Ke Za Zhi 60 (4), 345-349 (2022)
   PUBMED   35385942
  REMARK    GeneRIF: [Clinical phenotypes and genetic features of epilepsy
            children with MBD5 gene variants].
REFERENCE   3  (bases 1 to 10732)
  AUTHORS   Ohori,S., Tsuburaya,R.S., Kinoshita,M., Miyagi,E., Mizuguchi,T.,
            Mitsuhashi,S., Frith,M.C. and Matsumoto,N.
  TITLE     Long-read whole-genome sequencing identified a partial MBD5
            deletion in an exome-negative patient with neurodevelopmental
            disorder
  JOURNAL   J Hum Genet 66 (7), 697-705 (2021)
   PUBMED   33510365
  REMARK    GeneRIF: Long-read whole-genome sequencing identified a partial
            MBD5 deletion in an exome-negative patient with neurodevelopmental
            disorder.
REFERENCE   4  (bases 1 to 10732)
  AUTHORS   Le,T.N.U. and Ha,T.M.T.
  TITLE     MBD5-related intellectual disability in a Vietnamese child
  JOURNAL   Am J Med Genet A 185 (4), 1321-1323 (2021)
   PUBMED   33427406
  REMARK    GeneRIF: MBD5-related intellectual disability in a Vietnamese
            child.
REFERENCE   5  (bases 1 to 10732)
  AUTHORS   Gonzalez-Ortega,G., Llamas-Velasco,S., Arteche-Lopez,A.,
            Quesada-Espinosa,J.F., Puertas-Martin,V., Gomez-Grande,A.,
            Lopez-Alvarez,J., Saiz Diaz,R.A., Lezana-Rosales,J.M.,
            Villarejo-Galende,A. and Gonzalez de la Aleja,J.
  TITLE     Early-Onset Dementia Associated with a Heterozygous, Nonsense, and
            de novo Variant in the MBD5 Gene
  JOURNAL   J Alzheimers Dis 84 (1), 73-78 (2021)
   PUBMED   34459404
  REMARK    GeneRIF: Early-Onset Dementia Associated with a Heterozygous,
            Nonsense, and de novo Variant in the MBD5 Gene.
REFERENCE   6  (bases 1 to 10732)
  AUTHORS   Gonzales,P.A., Pisitkun,T., Hoffert,J.D., Tchapyjnikov,D.,
            Star,R.A., Kleta,R., Wang,N.S. and Knepper,M.A.
  TITLE     Large-scale proteomics and phosphoproteomics of urinary exosomes
  JOURNAL   J Am Soc Nephrol 20 (2), 363-379 (2009)
   PUBMED   19056867
REFERENCE   7  (bases 1 to 10732)
  AUTHORS   Wagenstaller,J., Spranger,S., Lorenz-Depiereux,B., Kazmierczak,B.,
            Nathrath,M., Wahl,D., Heye,B., Glaser,D., Liebscher,V.,
            Meitinger,T. and Strom,T.M.
  TITLE     Copy-number variations measured by single-nucleotide-polymorphism
            oligonucleotide arrays in patients with mental retardation
  JOURNAL   Am J Hum Genet 81 (4), 768-779 (2007)
   PUBMED   17847001
REFERENCE   8  (bases 1 to 10732)
  AUTHORS   Roloff,T.C., Ropers,H.H. and Nuber,U.A.
  TITLE     Comparative study of methyl-CpG-binding domain proteins
  JOURNAL   BMC Genomics 4 (1), 1 (2003)
   PUBMED   12529184
REFERENCE   9  (bases 1 to 10732)
  AUTHORS   Nakayama,M., Kikuno,R. and Ohara,O.
  TITLE     Protein-protein interactions between large proteins: two-hybrid
            screening using a functionally classified library composed of long
            cDNAs
  JOURNAL   Genome Res 12 (11), 1773-1784 (2002)
   PUBMED   12421765
REFERENCE   10 (bases 1 to 10732)
  AUTHORS   Mullegama,S.V., Mendoza-Londono,R. and Elsea,S.H.
  TITLE     MBD5 Haploinsufficiency
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   27786435
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC019226.5, AC018465.8,
            AC019070.7 and AC016731.7.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Jan 29, 2020 this sequence version replaced XM_024452987.1.
            
            Summary: This gene encodes a member of the methyl-CpG-binding
            domain (MBD) family. The MBD consists of about 70 residues and is
            the minimal region required for a methyl-CpG-binding protein
            binding specifically to methylated DNA. In addition to the MBD
            domain, this protein contains a PWWP domain (Pro-Trp-Trp-Pro
            motif), which consists of 100-150 amino acids and is found in
            numerous proteins that are involved in cell division, growth and
            differentiation. Mutations in this gene cause an autosomal dominant
            type of cognitive disability. The encoded protein interacts with
            the polycomb repressive complex PR-DUB which catalyzes the
            deubiquitination of a lysine residue of histone 2A.
            Haploinsufficiency of this gene is associated with a syndrome
            involving microcephaly, intellectual disabilities, severe speech
            impairment, and seizures. Alternatively spliced transcript variants
            have been found, but their full-length nature is not determined.
            [provided by RefSeq, Jul 2017].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR14038193.2939072.1,
                                           SRR14038191.4041307.1 [ECO:0000332]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000642680.2/ ENSP00000493871.2
            RefSeq Select criteria :: based on manual assertion, conservation
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-594               AC019226.5         65654-66247         c
            595-688             AC018465.8         75612-75705         c
            689-839             AC018465.8         21010-21160         c
            840-962             AC019070.7         4522-4644           c
            963-1631            AC016731.7         75575-76243         c
            1632-1734           AC016731.7         71762-71864         c
            1735-1915           AC016731.7         70527-70707         c
            1916-4036           AC016731.7         63985-66105         c
            4037-5062           AC016731.7         50311-51336         c
            5063-5271           AC016731.7         48496-48704         c
            5272-6480           AC016731.7         43852-45060         c
            6481-6554           AC016731.7         31937-32010         c
            6555-6630           AC016731.7         24311-24386         c
            6631-10732          AC016731.7         17475-21576         c
FEATURES             Location/Qualifiers
     source          1..10732
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="2"
                     /map="2q23.1"
     gene            1..10732
                     /gene="MBD5"
                     /gene_synonym="C2DELq23.1; DEL2Q23.1; MRD1"
                     /note="methyl-CpG binding domain protein 5"
                     /db_xref="GeneID:55777"
                     /db_xref="HGNC:HGNC:20444"
                     /db_xref="MIM:611472"
     exon            1..594
                     /gene="MBD5"
                     /gene_synonym="C2DELq23.1; DEL2Q23.1; MRD1"
                     /inference="alignment:Splign:2.1.0"
     exon            595..688
                     /gene="MBD5"
                     /gene_synonym="C2DELq23.1; DEL2Q23.1; MRD1"
                     /inference="alignment:Splign:2.1.0"
     exon            689..839
                     /gene="MBD5"
                     /gene_synonym="C2DELq23.1; DEL2Q23.1; MRD1"
                     /inference="alignment:Splign:2.1.0"
     exon            840..962
                     /gene="MBD5"
                     /gene_synonym="C2DELq23.1; DEL2Q23.1; MRD1"
                     /inference="alignment:Splign:2.1.0"
     exon            963..1631
                     /gene="MBD5"
                     /gene_synonym="C2DELq23.1; DEL2Q23.1; MRD1"
                     /inference="alignment:Splign:2.1.0"
     CDS             1519..6702
                     /gene="MBD5"
                     /gene_synonym="C2DELq23.1; DEL2Q23.1; MRD1"
                     /note="isoform 1 is encoded by transcript variant 1;
                     methyl-CpG-binding protein MBD5"
                     /codon_start=1
                     /product="methyl-CpG-binding domain protein 5 isoform 1"
                     /protein_id="NP_001365049.1"
                     /db_xref="CCDS:CCDS92873.1"
                     /db_xref="GeneID:55777"
                     /db_xref="HGNC:HGNC:20444"
                     /db_xref="MIM:611472"
                     /translation="MNGGKECDGGDKEGGLPAIQVPVGWQRRVDQNGVLYVSPSGSLL
                     SCLEQVKTYLLTDGTCKCGLECPLILPKVFNFDPGAAVKQRTAEDVKADEDVTKLCIH
                     KRKIIAVATLHKSMEAPHPSLVLTSPGGGTNATPVVPSRAATPRSVRNKSHEGITNSV
                     MPECKNPFKLMIGSSNAMGRLYVQELPGSQQQELHPVYPRQRLGSSEHGQKSPFRGSH
                     GGLPSPASSGSQIYGDGSISPRTDPLGSPDVFTRSNPGFHGAPNSSPIHLNRTPLSPP
                     SVMLHGSPVQSSCAMAGRTNIPLSPTLTTKSPVMKKPMCNFSTNMEIPRAMFHHKPPQ
                     GPPPPPPPSCALQKKPLTSEKDPLGILDPIPSKPVNQNPVIINPTSFHSNVHSQVPMM
                     NVSMPPAVVPLPSNLPLPTVKPGHMNHGSHVQRVQHSASTSLSPSPVTSPVHMMGTGI
                     GRIEASPQRSRSSSTSSDHGNFMMPPVGPQATSSGIKVPPRSPRSTIGSPRPSMPSSP
                     STKSDGHHQYKDIPNPLIAGISNVLNTPSSAAFPTASAGSSSVKSQPGLLGMPLNQIL
                     NQHNAASFPASSLLSAAAKAQLANQNKLAGNNSSSSSNSGAVAGSGNTEGHSTLNTMF
                     PPTANMLLPTGEGQSGRAALRDKLMSQQKDALRKRKQPPTTVLSLLRQSQMDSSAVPK
                     PGPDLLRKQGQGSFPISSMSQLLQSMSCQSSHLSSNSTPGCGASNTALPCSANQLHFT
                     DPSMNSSVLQNIPLRGEAVHCHNANTNFVHSNSPVPNHHLAGLINQIQASGNCGMLSQ
                     SGMALGNSLHPNPPQSRISTSSTPVIPNSIVSSYNQTSSEAGGSGPSSSIAIAGTNHP
                     AITKTTSVLQDGVIVTTAAGNPLQSQLPIGSDFPFVGQEHALHFPSNSTSNNHLPHPL
                     NPSLLSSLPISLPVNQQHLLNQNLLNILQPSAGEGKSEINLHPLGFLNPNVNAALAFL
                     SSDMDGQVLQPVHFQLLAALLQNQAQAAAMLPLPSFNLTISDLLQQQNTPLPSLTQMT
                     APPDHLPSNQSDNSRAETLLTSPLGNPLPSFAGSDTTFNPLFLPAVNGASGLMTLNPQ
                     LLGGVLNSASANTANHPEVSIATSSQATTTTTTTSSAVAALTVSTLGGTAVVSMAETL
                     LNISNNAGNTPGPAKLNSNSVVPQLLNPLLGTGLLGDMSSINNTLSNHQLTHLQSLLN
                     NNQMFPPNQQQQQLLQGYQNLQAFQGQSTIPCPANNNPMACLFQNFQVRMQEDAALLN
                     KRISTQPGLTALPENPNTTLPPFQDTPCELQPRIDPSLGQQVKDGLVVGGPGDASVDA
                     IYKAVVDAASKGMQVVITTAVNSTTQISPIPALSAMSAFTASIGDPLNLSSAVSAVIH
                     GRNMGGVDHDGRLRNSRGARLPKNLDHGKNVNEGDGFEYFKSASCHTSKKQWDGEQSP
                     RGERNRWKYEEFLDHPGHIHSSPCHERPNNVSTLPFLPGEQHPILLPPRNCPGDKILE
                     ENFRYNNYKRTMMSFKERLENTVERCAHINGNRPRQSRGFGELLSTAKQDLVLEEQSP
                     SSSNSLENSLVKDYIHYNGDFNAKSVNGCVPSPSDAKSISSEDDLRNPDSPSSNELIH
                     YRPRTFNVGDLVWGQIKGLTSWPGKLVREDDVHNSCQQSPEEGKVEPEKLKTLTEGLE
                     AYSRVRKRNRKSGKLNNHLEAAIHEAMSELDKMSGTVHQIPQGDRQMRPPKPKRRKIS
                     R"
     exon            1632..1734
                     /gene="MBD5"
                     /gene_synonym="C2DELq23.1; DEL2Q23.1; MRD1"
                     /inference="alignment:Splign:2.1.0"
     exon            1735..1915
                     /gene="MBD5"
                     /gene_synonym="C2DELq23.1; DEL2Q23.1; MRD1"
                     /inference="alignment:Splign:2.1.0"
     exon            1916..4036
                     /gene="MBD5"
                     /gene_synonym="C2DELq23.1; DEL2Q23.1; MRD1"
                     /inference="alignment:Splign:2.1.0"
     exon            4037..5062
                     /gene="MBD5"
                     /gene_synonym="C2DELq23.1; DEL2Q23.1; MRD1"
                     /inference="alignment:Splign:2.1.0"
     exon            5063..5271
                     /gene="MBD5"
                     /gene_synonym="C2DELq23.1; DEL2Q23.1; MRD1"
                     /inference="alignment:Splign:2.1.0"
     exon            5272..6480
                     /gene="MBD5"
                     /gene_synonym="C2DELq23.1; DEL2Q23.1; MRD1"
                     /inference="alignment:Splign:2.1.0"
     exon            6481..6554
                     /gene="MBD5"
                     /gene_synonym="C2DELq23.1; DEL2Q23.1; MRD1"
                     /inference="alignment:Splign:2.1.0"
     exon            6555..6630
                     /gene="MBD5"
                     /gene_synonym="C2DELq23.1; DEL2Q23.1; MRD1"
                     /inference="alignment:Splign:2.1.0"
     exon            6631..10732
                     /gene="MBD5"
                     /gene_synonym="C2DELq23.1; DEL2Q23.1; MRD1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      7197..7202
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MBD5"
                     /gene_synonym="C2DELq23.1; DEL2Q23.1; MRD1"
                     /note="hexamer: AATAAA"
     regulatory      7203..7208
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MBD5"
                     /gene_synonym="C2DELq23.1; DEL2Q23.1; MRD1"
                     /note="hexamer: AATACA"
     polyA_site      7228
                     /gene="MBD5"
                     /gene_synonym="C2DELq23.1; DEL2Q23.1; MRD1"
                     /note="major polyA site"
     polyA_site      7238
                     /gene="MBD5"
                     /gene_synonym="C2DELq23.1; DEL2Q23.1; MRD1"
                     /note="major polyA site"
     regulatory      10711..10716
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MBD5"
                     /gene_synonym="C2DELq23.1; DEL2Q23.1; MRD1"
                     /note="hexamer: AATACA"
     polyA_site      10732
                     /gene="MBD5"
                     /gene_synonym="C2DELq23.1; DEL2Q23.1; MRD1"
                     /note="major polyA site"
ORIGIN      
        1 aagcagagag accccagcag cagcagcagc tgatgatgaa gagagaggca gtggcagagg
       61 gggggcacct tttatttcta tttttaaagg gacaggacac taattctacc ccacttcaac
      121 cttgaattca ggggggtggg gggaaggcgg ctgagttcct tcccccaccc tccagccctg
      181 agccctgaga gggggattga gcctgagaga ggagaaggag tttcttcttc ttcgaaaacc
      241 cccatccacg actcctaccc cctcacccct ccaactcgcc tccctccctc caccctcctc
      301 ctctttggcc gtgagaggag gagagaaaga aaccaaaagc ctcttagcaa cacagaccct
      361 ttgctgctgc tgttgctgct gctgctgctg ttgctgctgc tgctgctact gctgctgctg
      421 ctactgctgc tgcttggccc tggctggaga catctcacta cacccaggag cagccacttc
      481 cccagctctc ctcctcctcc ttcgcctcct cctcctccac tccccccctt tattaccctt
      541 tgtgtcatcc tccacagctc cagggaaggc actcaaaagt ggggggcagg aaagatgtac
      601 attgaagatg tcagtttcta catgtgggaa gtagacattg aaggctttat ggtttacaga
      661 caagatcttt agaagataag cactaaagga ccaaggaaat aaataccaga aatcaagaag
      721 agcacacact attttccttc atcaatccat agaaccctaa tgcttcttta gttccatcaa
      781 acacagagga atgaccacca tggcagatgg caacagagga tgtcaacaat tttttggaga
      841 gagaagaggt actcccttat agggactcgt aaagacatag agcatctgga aattaactgc
      901 ctccattaca ctgctgagtc acacacaacg cttgttatgt tttcatcact tctctccaga
      961 aggctacatt attggaattt tgaagtcatg aaaacatcag atgaaccaac ctgcaacacc
     1021 ttggattcag attggaagat aaagagaaag tttaaagaat gtggcctata aaggcgggta
     1081 cctggaaata ttaaccgact cacactgtaa aaatgagacc agtttctaaa caatagagat
     1141 tgtcttagta caacatcaag gttcaagaca aatgttgaac taaaaacttt acactcccca
     1201 cccccacttc agacaggtac cagcattggt gaattatgat tttccttagt cagaagcact
     1261 catttttacc catgtagacc atccttagga attaaatatt ggttatttaa tgtagattat
     1321 aatgggaagc tgattttttt cacaatggca tatttcaagg acttggttcc aaactgagct
     1381 gaagcttccc aatgcgtttt gtacagtctg ggaaaaactg tgctgcactg gcccactttt
     1441 gaaggccatc atgctctgta atataaggat atcatcttat tgctgatatc tttggagagt
     1501 ccctagcaga cacagaaaat gaatggaggc aaagagtgtg acggagggga caaggaagga
     1561 ggtcttccag ctatacaagt tcctgtgggt tggcagcgtc gtgtggatca aaatggagtg
     1621 ctttatgtca gtcccagtgg gtctttgtta tcttgcttgg agcaggttaa aacatacctg
     1681 cttactgatg gaacatgcaa gtgtggcttg gaatgtcctc ttattcttcc caaggtattt
     1741 aattttgatc ctggagctgc tgtgaaacag agaaccgcag aagatgttaa ggcagatgaa
     1801 gatgtcacaa agctatgcat acataaaaga aaaattattg cagtggccac acttcataaa
     1861 agcatggaag ccccacatcc ttctctggtg ctcaccagtc ccggaggagg aacaaatgca
     1921 actccagtag taccttctcg ggcagcaact ccaagatcag taagaaataa gtctcatgaa
     1981 ggaattacaa attctgtaat gcctgaatgt aagaatcctt tcaagttaat gattggatca
     2041 tcaaatgcca tgggaaggct atatgtacaa gaactgcctg gaagccaaca acaagaactc
     2101 caccctgtct acccccgaca gagattgggc agcagtgaac atggacagaa atctccattc
     2161 cgtggcagcc atggaggcct gcccagccca gcgtcatcag gttcccagat atatggagat
     2221 ggttcaatct ctccaaggac tgacccactt ggaagtcctg atgttttcac aagaagtaat
     2281 cctggttttc atggagctcc caattctagt cctattcacc tgaataggac tcctctttct
     2341 ccaccttcag taatgctaca tggttctcct gtacagtcat cctgtgcaat ggctggaagg
     2401 actaatatac ctctttcccc aaccttgact acaaagagtc cagtaatgaa aaaaccaatg
     2461 tgtaattttt caactaatat ggaaatacca cgagcaatgt tccaccacaa accaccccaa
     2521 ggcccacctc cccctcctcc accttcttgt gctcttcaga aaaagccatt aacatctgag
     2581 aaagatccac ttggcattct tgaccctatt cctagtaaac cagtgaatca gaaccctgtt
     2641 atcattaatc caaccagttt ccattcaaat gtccactctc aggtacctat gatgaatgta
     2701 agcatgcctc ctgctgttgt tcctttgcca agtaatctcc cattgccaac tgtaaaacct
     2761 ggtcacatga atcatgggag tcatgtacaa agagttcagc attcagcttc aacctccctg
     2821 tccccttctc cagtgacatc ccccgtgcac atgatgggga ctggaattgg aaggattgag
     2881 gcatcgcccc aaagatcacg ctcatcttcc acatcatcag atcatggaaa tttcatgatg
     2941 ccacctgtag gaccccaggc cacttctagt ggtattaagg ttccacccag gtcaccaagg
     3001 tcaacaatag ggtccccaag gccatcaatg ccatcaagcc cttctaccaa gtccgatgga
     3061 catcatcagt acaaggatat ccctaaccca ttaattgctg gaataagtaa tgtactaaat
     3121 accccaagca gtgcagcttt tcctactgca tctgccggaa gtagttctgt aaagagtcag
     3181 cctggtttgc tgggaatgcc tttaaatcag atcttgaacc agcacaatgc tgcctccttt
     3241 ccagcaagta gtttactctc agcagcagcc aaagcacagc tagcaaatca aaacaaactt
     3301 gctggtaaca acagtagcag cagtagcaat tctggagctg ttgccggcag tggcaacact
     3361 gaaggacata gcactttaaa caccatgttc cctcctactg ccaacatgct tctcccaaca
     3421 ggtgaagggc aaagtggtcg agcagcacta agagataagc tgatgtctca gcaaaaagac
     3481 gcattgcgga aaagaaaaca accacctacg acagtgttga gtttgctcag acagtctcaa
     3541 atggatagtt ctgcagttcc taaacctgga cctgacttgc taaggaagca gggtcagggt
     3601 tcatttccca tcagttcaat gtctcagtta ctacagtcta tgagttgtca aagctctcac
     3661 ttgagtagca atagtacccc gggttgtggg gcctcaaata ctgctttgcc ttgctctgct
     3721 aaccagctgc attttacaga tcccagtatg aactctagtg ttcttcagaa cataccttta
     3781 agaggggaag ccgtgcactg ccacaatgca aacactaact ttgttcacag taacagtcca
     3841 gtccccaacc accatcttgc aggtttaata aatcagattc aggctagcgg gaactgtggg
     3901 atgctcagtc agtcgggcat ggctttagga aattccttac atcccaatcc acctcagtca
     3961 agaatttcaa cgtcctccac tccagtgata ccaaacagca ttgttagcag ctataatcaa
     4021 acaagttctg aagcaggcgg ttcaggacca tcatcctcca tagccatagc gggcaccaac
     4081 caccctgcca tcacaaagac aacatctgtt cttcaagatg gcgtcatagt caccactgca
     4141 gctggaaacc cactgcagag tcagctaccc attgggagtg attttccttt tgttggccag
     4201 gagcacgcac ttcattttcc atccaacagc acttcaaaca accatcttcc acaccccttg
     4261 aaccccagcc tcctcagttc tctacctatc tctttgccag tgaatcaaca gcatctccta
     4321 aaccagaatc tattaaatat cctccagcct tcagcaggag aaggcaagtc tgagatcaac
     4381 ctccaccctt taggttttct caacccgaat gtaaacgctg ctttagcttt tctctccagt
     4441 gacatggatg ggcaggtatt gcagcctgtt cactttcagc tcttagcagc cttgcttcag
     4501 aaccaagccc aagcagctgc catgcttccc ctgccatctt tcaatctgac catctcagat
     4561 cttttgcaac agcaaaatac ccctttaccc tcattaacac agatgacagc cccaccagac
     4621 catttgccaa gcaatcagtc agacaacagc cgagctgaga cccttttaac cagccccctg
     4681 gggaaccctt taccaagctt tgcaggcagt gacactactt ttaaccccct gttcctccca
     4741 gctgtcaatg gggcctcagg attaatgacc ttgaatcccc agctgttggg aggtgtcctg
     4801 aactcggcat cggccaacac cgctaatcat ccagaggttt ccatagcaac ctcctcccag
     4861 gcaaccacta ccacaaccac tacatcatca gcagtggcag cactgactgt ctcaacactt
     4921 ggtgggacag cagtggtgtc aatggcagaa acattgctga atatatctaa taatgctggg
     4981 aatacacctg gtccagctaa actcaacagt aactctgtgg tgccacagct acttaaccct
     5041 ctactgggga caggtctact tggtgatatg tcatcaataa acaatacttt gagtaaccat
     5101 caactgactc atctacagtc gctgttaaac aacaatcaga tgtttcctcc aaatcagcaa
     5161 cagcagcaac ttctccaggg gtaccagaat ctccaggcgt tccaaggaca gtccacaatt
     5221 ccttgcccag ctaacaataa ccccatggct tgtctgtttc agaactttca ggtgagaatg
     5281 caggaagatg cagctctcct aaacaaaaga ataagcactc agcctgggct cacagcactt
     5341 cctgagaatc caaacactac acttccacct tttcaagata caccttgtga gttgcaaccg
     5401 aggattgacc catctcttgg tcaacaggtg aaggatggcc tcgttgtggg tggcccaggt
     5461 gatgcttccg tagatgccat ttacaaagca gttgtcgatg cagccagcaa aggaatgcag
     5521 gttgtcatca ccactgcagt caacagtaca actcagatca gccccattcc agctctgagt
     5581 gccatgagtg ccttcactgc ctcaattggt gacccattaa atctctccag tgctgtcagt
     5641 gcggtcattc atggacggaa catgggaggt gttgatcatg atggtaggct gaggaattca
     5701 agaggggctc ggctgcccaa gaatctagac catgggaaaa atgtgaacga aggagatggg
     5761 tttgaatatt tcaagtcagc aagttgccac acatccaaaa aacagtggga cggggagcaa
     5821 agccccagag gggagcgaaa caggtggaag tacgaggaat ttttagatca tccaggccat
     5881 atccacagta gtccttgtca tgaaaggccc aacaatgtct ctacactgcc atttctgcct
     5941 ggggaacagc acccaatact gttaccacca agaaactgtc caggggataa aattctagag
     6001 gaaaatttca ggtataataa ctacaaaaga actatgatga gttttaagga gagactagag
     6061 aacactgtgg aaagatgtgc acacataaat gggaatagac ctcgacagag tcggggattt
     6121 ggagagctgc taagcactgc aaagcaagac ctggtcctag aggagcagtc tccaagttcc
     6181 tcaaatagtt tggaaaattc tctggtcaaa gactacatcc attacaatgg agactttaat
     6241 gccaaaagcg ttaatgggtg tgtgcctagc ccttcagatg ctaaaagcat tagtagtgaa
     6301 gatgacctaa ggaacccaga ctccccctct tcaaatgaat tgatacatta tagaccaagg
     6361 acgttcaatg ttggcgactt ggtctggggc caaatcaaag gactgacttc ctggcctgga
     6421 aaattagtaa gagaagacga cgttcacaat tcatgtcaac aaagccccga ggaagggaag
     6481 gtggagcccg agaagttgaa gacactaaca gaaggtttgg aagcctacag ccgtgtccgg
     6541 aaaaggaaca gaaaaagtgg aaagctaaat aaccatttag aagctgctat tcatgaggcc
     6601 atgagtgaac tggacaaaat gtctgggact gtacaccaaa tcccacaggg tgacagacaa
     6661 atgagacccc ccaaacccaa gaggaggaag atctccagat aacagagact actccactaa
     6721 tgcgcagtgt ttattaaagg aacatgcaca gatgtatctg tatataggta ttgatatagc
     6781 cacagttata tcaatattta gactatggca gatagctacc accaccacag ggtgctaaaa
     6841 gaaacagtga tacaaaattt ttttgatcag gaaggataat gaatgctgga aaagccaatc
     6901 aaagtctctg tgtgatgaga gtgatcaatg gtcaagagat tactgagaaa ctctttttct
     6961 ataatactaa attgtgatta taagaaatag tccaaatgtt tctgaagaat tttcaatgtt
     7021 gtatatagaa aatacagaat ttcatggtga tatcagaaat gtaaatattg tacagtattg
     7081 tcatgtacaa gcgtacctaa tgcacacttt atcttttatt gtacaaagaa atagtgtaca
     7141 aaattctttg gtttctatgg agtacaccta ccagagacta ccagtgtaaa gtgataaata
     7201 aaaatacaac ctaaatgctt ttctatgata atgtaaaaga tgctgttttt gtatttaaca
     7261 gcagagaaaa ggcatgatga tgtaatatct gaattatgac atacactgca caccactgag
     7321 tgtccattta tattgtctgt ttggaatgaa ccttgcctgg aagcactgga ctcaatttcg
     7381 ggtgtctagg aaattgcaac tttgcagatt tctaaaggga tgagggctca caggtctaaa
     7441 ttaagaattc agaaactgca accatttgtt gcatattttt tttacctaag ttttaaaata
     7501 gaataggttt tataaaaaaa aatcttagat ggaatatttt actcagccat ttctgtagtt
     7561 aacatttgat agccacctgt tgaagcagat agcttatact gactgcacca ccgctggtgc
     7621 tcagaattga gggttttttt gcaaatgata tctgacaagg taaaactttc tatttccgta
     7681 catggaggca aaaaccccac tttgcaacca ttatcagagg taagattgat aataattcat
     7741 ttttttgtag tggcaaatat aaatatgaat catcaaagca agtttcatat ccattcatca
     7801 gtattactta acccagaagt taaggtggct tacggaatta ttagcaatgg taattttttt
     7861 atcagtattg tgtaacatat cagatttatt ttatttaaag aattatttat accattaaca
     7921 gattcttata tatattaatg tggcaaaaat gtgcaggtta aatctattaa ccaaattatt
     7981 tatattatat taagtaagaa aaaatgtgaa acaaatgtag agagaaaata ctacattaca
     8041 agtatacaaa cctaaaactg tgagccattg taaagtacaa ggtaattaat aagaaatgta
     8101 gcacaacata attttccgtc ttcttctcac aatttttgtg gtggtggtat taccctatct
     8161 tccaggcagt taagcaaagt ttaggcttcc agcctgactt cagttgatgg attgtcaaaa
     8221 gaagggatct attatccctt tattcttgga accaccagtc cctgccccaa acccttaaac
     8281 ccctagagag cactgcctcg tcctctagcc tggggctcac acaacagtct gccactcagg
     8341 ctgctaggag attctccaat agcaggctca gcacctcaaa ctgcccacca cctctcctac
     8401 tgctcctctc ccacccacca cccaaactcc tcagtcctgt catgtagcag agttccagta
     8461 actcaggaaa atggagacca aggtcattcc ttaagttggt ttcttggggc cacagcacag
     8521 agcatgccac ctgggctcac ttttgatagc aacagctctt agttattccc ctaggtctcc
     8581 tgtgtctctc tgtccctctg ttcctacttt caacctaccc ccaagcctga tggaatgcaa
     8641 ctggctaccc tgggcttcat gacctcaaag taggaaccct tcgttctggg ggtaggttca
     8701 ttttgtctct cttgtaatgg tgggattgcc tgcccagttc cttcatgcag tcacatgaag
     8761 taatcttttg tgtagtttag ttgacacatg tttataagtt aaaagttgat gtggctagta
     8821 tgtttaaacc attaatgtcc attttttttg caccaacctg tttttctact gttttttcct
     8881 acacattttt gtttattcac agacagacag acatgtgtgt ttgtatgctg ggtgtgtctg
     8941 tatgtgtgta tgtgttgata tgtaaatttt ttagcctttg cttttctgtc ttcaggctga
     9001 gtttgtgttg accaagcctt ctttttggta ttttgagcta attcagtttg tgcttccctc
     9061 ccattgtata ctttacataa gtagaccatg taaagtatgt ttgtgcccat gatttcactg
     9121 gttgctatgg ctttgatatg ggtgctaggt gtagtagttt cagtccacgt gggttttttt
     9181 catctctctt agagttatat ggttttgtgt aagcagttaa tgtaaatatt gttagcatta
     9241 caggtcatac agtgctgtaa cattttttta aatgttgcac ctactttaca attaaaaaat
     9301 tggtcactcg cacttcaatt ttgcccatag agccatttct ttgtctttgt attgaagcca
     9361 attcattttt ttaaatgaag gctaggcctt ctatgttacc aaaattttta ttccctaaaa
     9421 taaacttttt aagaaacaaa tccaagaatg gaaacagatg aaaaattttt tcttctttaa
     9481 agaacataat agttcctgct ggaaaaggaa atctaatttt attttgtgtc ccttaacagt
     9541 cctaggaagc acaaggagaa taaactaaac ctctaagaag gttttaaaaa ttatttcaaa
     9601 tgagtgaaaa ttaactgagc agcttttgtg tgatttgtct tgtttgtagc attaaaagca
     9661 aaccagggtt tttatttatt taaaggaaca tttttggctt gtacttttca gtgccattat
     9721 gaatgaaaat gttttaagaa cattcatccc ttgtgatatc atgggccact tttagtcttt
     9781 tatttggacc ctgactttga gtttttgcta tgcctgtttt ttaagtaaac acagccttct
     9841 tatttgaacg taacactgca gaattgcagg aagagaaaag ggcagttagt tatttcactt
     9901 tctgtaagtt ttaggaagat tcttatatcc tacgaggtga ctcatgtttc attttagacc
     9961 atgatagcac attgttgtta actttggtaa tcttccatgc agtttgcagt gttcccttgc
    10021 aagtcacaag catatatgca ttaagatttt aaaatctaag atgcttaaga tgttggtggc
    10081 attgctcttt ctgaaaagaa agacaaaata ctagaccttg ctgaaaattg gatatcttat
    10141 ttactttgcc catttatgcc cgaatggtgt aatgcaagac tcaagacttt tcaatgtagt
    10201 atatcaaatg agttgtgcat tgattcattt gggggacatg aacaaaaatt atcaaccagc
    10261 cagaactgta atatttaatt agttcatctt tttctttcat tcactctatc acatttgcat
    10321 tagccaaaga gataagaaca ttagtaattc atatgtgtaa acaacccaga agtgaatatt
    10381 aaatcagtga gccaaaaagc aataaacaac tcgagcatgg gaaatcctgc taacagtggg
    10441 gagtctgata tagaaaaaat atataaaaag cattatcttc aaatatgaaa gattaatttg
    10501 tatctactgt aaatatgtat taccagcctt atctgcattt atacacactg tgtgtgtacc
    10561 tcagtgacct tttataaacc ggaaaaatgg ttgatttaat aatttgttat gtaaatacaa
    10621 agttgtctat aaattggaaa atttgatgcc agatggcttc ataatataca aatgtgtttt
    10681 gtaacatcat gcctttatgg attaagtata aatacactgg attgtctatg ta
//
