LOCUS       NM_003927               5064 bp    mRNA    linear   PRI 04-APR-2024
DEFINITION  Homo sapiens methyl-CpG binding domain protein 2 (MBD2), transcript
            variant 1, mRNA.
ACCESSION   NM_003927
VERSION     NM_003927.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5064)
  AUTHORS   Duan,W., Huang,J., Wasti,B., Chen,Z., Yuan,Y., He,Y., Li,D.,
            Jia,J., Liu,S., Liu,Y., Ma,L., Zeng,Q., Zhu,L., Li,J., Zhang,X. and
            Xiang,X.
  TITLE     miR-146a-3p as a potential novel therapeutic by targeting MBD2 to
            mediate Th17 differentiation in Th17 predominant neutrophilic
            severe asthma
  JOURNAL   Clin Exp Med 23 (6), 2839-2854 (2023)
   PUBMED   36961677
  REMARK    GeneRIF: miR-146a-3p as a potential novel therapeutic by targeting
            MBD2 to mediate Th17 differentiation in Th17 predominant
            neutrophilic severe asthma.
REFERENCE   2  (bases 1 to 5064)
  AUTHORS   Shao,X., Zheng,Y., Huang,Y., Li,G., Zou,W. and Shi,L.
  TITLE     Hsa-miR-221-3p promotes proliferation and migration in
            HER2-positive breast cancer cells by targeting LASS2 and MBD2
  JOURNAL   Histol Histopathol 37 (11), 1099-1112 (2022)
   PUBMED   35734966
  REMARK    GeneRIF: Hsa-miR-221-3p promotes proliferation and migration in
            HER2-positive breast cancer cells by targeting LASS2 and MBD2.
REFERENCE   3  (bases 1 to 5064)
  AUTHORS   Leighton,G.O., Irvin,E.M., Kaur,P., Liu,M., You,C., Bhattaram,D.,
            Piehler,J., Riehn,R., Wang,H., Pan,H. and Williams,D.C. Jr.
  TITLE     Densely methylated DNA traps Methyl-CpG-binding domain protein 2
            but permits free diffusion by Methyl-CpG-binding domain protein 3
  JOURNAL   J Biol Chem 298 (10), 102428 (2022)
   PUBMED   36037972
  REMARK    GeneRIF: Densely methylated DNA traps Methyl-CpG-binding domain
            protein 2 but permits free diffusion by Methyl-CpG-binding domain
            protein 3.
REFERENCE   4  (bases 1 to 5064)
  AUTHORS   Wu,G.R., Zhou,M., Wang,Y., Zhou,Q., Zhang,L., He,L., Zhang,S.,
            Yu,Q., Xu,Y., Zhao,J., Xiong,W. and Wang,C.Y.
  TITLE     Blockade of Mbd2 by siRNA-loaded liposomes protects mice against
            OVA-induced allergic airway inflammation via repressing M2
            macrophage production
  JOURNAL   Front Immunol 13, 930103 (2022)
   PUBMED   36090987
  REMARK    GeneRIF: Blockade of Mbd2 by siRNA-loaded liposomes protects mice
            against OVA-induced allergic airway inflammation via repressing M2
            macrophage production.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 5064)
  AUTHORS   Xie,Y., Wang,F., Yu,J., Zhang,J., Liu,Y., Li,M. and Qi,J.
  TITLE     Silencing of MBD2 and EZH2 inhibits the proliferation of colorectal
            carcinoma cells by rescuing the expression of SFRP
  JOURNAL   Oncol Rep 46 (6) (2021)
   PUBMED   34617573
  REMARK    GeneRIF: Silencing of MBD2 and EZH2 inhibits the proliferation of
            colorectal carcinoma cells by rescuing the expression of SFRP.
REFERENCE   6  (bases 1 to 5064)
  AUTHORS   Bhattacharya,S.K., Ramchandani,S., Cervoni,N. and Szyf,M.
  TITLE     A mammalian protein with specific demethylase activity for mCpG DNA
  JOURNAL   Nature 397 (6720), 579-583 (1999)
   PUBMED   10050851
REFERENCE   7  (bases 1 to 5064)
  AUTHORS   Hendrich,B. and Bird,A.
  TITLE     Identification and characterization of a family of mammalian
            methyl-CpG binding proteins
  JOURNAL   Mol Cell Biol 18 (11), 6538-6547 (1998)
   PUBMED   9774669
REFERENCE   8  (bases 1 to 5064)
  AUTHORS   Tong,J.K., Hassig,C.A., Schnitzler,G.R., Kingston,R.E. and
            Schreiber,S.L.
  TITLE     Chromatin deacetylation by an ATP-dependent nucleosome remodelling
            complex
  JOURNAL   Nature 395 (6705), 917-921 (1998)
   PUBMED   9804427
REFERENCE   9  (bases 1 to 5064)
  AUTHORS   Zhang,Y., LeRoy,G., Seelig,H.P., Lane,W.S. and Reinberg,D.
  TITLE     The dermatomyositis-specific autoantigen Mi2 is a component of a
            complex containing histone deacetylase and nucleosome remodeling
            activities
  JOURNAL   Cell 95 (2), 279-289 (1998)
   PUBMED   9790534
REFERENCE   10 (bases 1 to 5064)
  AUTHORS   Scanlan,M.J., Chen,Y.T., Williamson,B., Gure,A.O., Stockert,E.,
            Gordan,J.D., Tureci,O., Sahin,U., Pfreundschuh,M. and Old,L.J.
  TITLE     Characterization of human colon cancer antigens recognized by
            autologous antibodies
  JOURNAL   Int J Cancer 76 (5), 652-658 (1998)
   PUBMED   9610721
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AF072242.1, AC090666.6 and
            AW151547.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_003927.4.
            
            Summary: DNA methylation is the major modification of eukaryotic
            genomes and plays an essential role in mammalian development. Human
            proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of
            nuclear proteins related by the presence in each of a methyl-CpG
            binding domain (MBD). Each of these proteins, with the exception of
            MBD3, is capable of binding specifically to methylated DNA. MECP2,
            MBD1 and MBD2 can also repress transcription from methylated gene
            promoters. The protein encoded by this gene may function as a
            mediator of the biological consequences of the methylation signal.
            It is also reported that the this protein functions as a
            demethylase to activate transcription, as DNA methylation causes
            gene silencing. Two transcript variants encoding different isoforms
            have been found for this gene. [provided by RefSeq, Feb 2011].
            
            Transcript Variant: This variant (1) encodes the longer isoform
            (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF072242.1, SRR1163655.427425.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2142348, SAMEA2148093
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000256429.8/ ENSP00000256429.3
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1384              AF072242.1         120-1503
            1385-5028           AC090666.6         36177-39820
            5029-5064           AW151547.1         8-43                c
FEATURES             Location/Qualifiers
     source          1..5064
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="18"
                     /map="18q21.2"
     gene            1..5064
                     /gene="MBD2"
                     /gene_synonym="DMTase; NY-CO-41"
                     /note="methyl-CpG binding domain protein 2"
                     /db_xref="GeneID:8932"
                     /db_xref="HGNC:HGNC:6917"
                     /db_xref="MIM:603547"
     exon            1..652
                     /gene="MBD2"
                     /gene_synonym="DMTase; NY-CO-41"
                     /inference="alignment:Splign:2.1.0"
     CDS             111..1346
                     /gene="MBD2"
                     /gene_synonym="DMTase; NY-CO-41"
                     /note="isoform 1 is encoded by transcript variant 1;
                     demethylase"
                     /codon_start=1
                     /product="methyl-CpG-binding domain protein 2 isoform 1"
                     /protein_id="NP_003918.1"
                     /db_xref="CCDS:CCDS11953.1"
                     /db_xref="GeneID:8932"
                     /db_xref="HGNC:HGNC:6917"
                     /db_xref="MIM:603547"
                     /translation="MRAHPGGGRCCPEQEEGESAAGGSGAGGDSAIEQGGQGSALAPS
                     PVSGVRREGARGGGRGRGRWKQAGRGGGVCGRGRGRGRGRGRGRGRGRGRGRPPSGGS
                     GLGGDGGGCGGGGSGGGGAPRREPVPFPSGSAGPGPRGPRATESGKRMDCPALPPGWK
                     KEEVIRKSGLSAGKSDVYYFSPSGKKFRSKPQLARYLGNTVDLSSFDFRTGKMMPSKL
                     QKNKQRLRNDPLNQNKGKPDLNTTLPIRQTASIFKQPVTKVTNHPSNKVKSDPQRMNE
                     QPRQLFWEKRLQGLSASDVTEQIIKTMELPKGLQGVGPGSNDETLLSAVASALHTSSA
                     PITGQVSAAVEKNPAVWLNTSQPLCKAFIVTDEDIRKQEERVQQVRKKLEEALMADIL
                     SRAADTEEMDIEMDSGDEA"
     exon            653..812
                     /gene="MBD2"
                     /gene_synonym="DMTase; NY-CO-41"
                     /inference="alignment:Splign:2.1.0"
     exon            813..950
                     /gene="MBD2"
                     /gene_synonym="DMTase; NY-CO-41"
                     /inference="alignment:Splign:2.1.0"
     exon            951..1041
                     /gene="MBD2"
                     /gene_synonym="DMTase; NY-CO-41"
                     /inference="alignment:Splign:2.1.0"
     exon            1042..1219
                     /gene="MBD2"
                     /gene_synonym="DMTase; NY-CO-41"
                     /inference="alignment:Splign:2.1.0"
     exon            1220..1358
                     /gene="MBD2"
                     /gene_synonym="DMTase; NY-CO-41"
                     /inference="alignment:Splign:2.1.0"
     exon            1359..5064
                     /gene="MBD2"
                     /gene_synonym="DMTase; NY-CO-41"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1811..1816
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MBD2"
                     /gene_synonym="DMTase; NY-CO-41"
                     /note="hexamer: AATAAA"
     polyA_site      1830
                     /gene="MBD2"
                     /gene_synonym="DMTase; NY-CO-41"
                     /note="major polyA site"
     regulatory      5043..5048
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MBD2"
                     /gene_synonym="DMTase; NY-CO-41"
                     /note="hexamer: AATAAA"
     polyA_site      5064
                     /gene="MBD2"
                     /gene_synonym="DMTase; NY-CO-41"
ORIGIN      
        1 atgatgcttg cgcgcgtccc ccgcgcgccg cgctgcgggc ggggcgggtc tccgggattc
       61 caagggctcg gttacggaag aagcgcagcg ccggctgggg agggggctgg atgcgcgcgc
      121 acccgggggg aggccgctgc tgcccggagc aggaggaggg ggagagtgcg gcgggcggca
      181 gcggcgctgg cggcgactcc gccatagagc aggggggcca gggcagcgcg ctcgccccgt
      241 ccccggtgag cggcgtgcgc agggaaggcg ctcggggcgg cggccgtggc cgggggcggt
      301 ggaagcaggc gggccggggc ggcggcgtct gtggccgtgg ccggggccgg ggccgtggcc
      361 ggggacgggg acggggccgg ggccggggcc gcggccgtcc cccgagtggc ggcagcggcc
      421 ttggcggcga cggcggcggc tgcggcggcg gcggcagcgg tggcggcggc gccccccggc
      481 gggagccggt ccctttcccg tcggggagcg cggggccggg gcccagggga ccccgggcca
      541 cggagagcgg gaagaggatg gattgcccgg ccctcccccc cggatggaag aaggaggaag
      601 tgatccgaaa atctgggcta agtgctggca agagcgatgt ctactacttc agtccaagtg
      661 gtaagaagtt cagaagcaag cctcagttgg caaggtacct gggaaatact gttgatctca
      721 gcagttttga cttcagaact ggaaagatga tgcctagtaa attacagaag aacaaacaga
      781 gactgcgaaa cgatcctctc aatcaaaata agggtaaacc agacttgaat acaacattgc
      841 caattagaca aacagcatca attttcaaac aaccggtaac caaagtcaca aatcatccta
      901 gtaataaagt gaaatcagac ccacaacgaa tgaatgaaca gccacgtcag cttttctggg
      961 agaagaggct acaaggactt agtgcatcag atgtaacaga acaaattata aaaaccatgg
     1021 aactacccaa aggtcttcaa ggagttggtc caggtagcaa tgatgagacc cttttatctg
     1081 ctgttgccag tgctttgcac acaagctctg cgccaatcac agggcaagtc tccgctgctg
     1141 tggaaaagaa ccctgctgtt tggcttaaca catctcaacc cctctgcaaa gcttttattg
     1201 tcacagatga agacatcagg aaacaggaag agcgagtaca gcaagtacgc aagaaattgg
     1261 aagaagcact gatggcagac atcttgtcgc gagctgctga tacagaagag atggatattg
     1321 aaatggacag tggagatgaa gcctaagaat atgatcaggt aactttcgac cgactttccc
     1381 caagagaaaa ttcctagaaa ttgaacaaaa atgtttccac tggcttttgc ctgtaagaaa
     1441 aaaaatgtac ccgagcacat agagcttttt aatagcacta accaatgcct ttttagatgt
     1501 atttttgatg tatatatcta ttattcaaaa aatcatgttt attttgagtc ctaggactta
     1561 aaattagtct tttgtaatat caagcaggac cctaagatga agctgagctt ttgatgccag
     1621 gtgcaatcta ctggaaatgt agcacttacg taaaacattt gtttccccca cagttttaat
     1681 aagaacagat caggaattct aaataaattt cccagttaaa gattattgtg acttcactgt
     1741 atataaacat atttttatac tttattgaaa ggggacacct gtacattctt ccatcatcac
     1801 tgtaaagaca aataaatgat tatattcaca gactgattgg aattctttct gttgaaaagc
     1861 acacacaata aagaacccct cgttagcctt cctctgattt acattcaact ctgatccctg
     1921 ggccttaggt ttgacatgga ggtggaggaa gatagcgcat atatttgcag tatgaactat
     1981 tgcctctgga cgttgtgaga attgtgcttt caccagaatt tctaagaatt tctgctaaat
     2041 atcacctagc atgtgtaatt ttttttcctt gcctgtgact tggacttttg atagttctat
     2101 aagaataagg ctttttcttc ccttgggcat gagtcagata cacaaggacc cttcaggtgt
     2161 tactagaagg cgtccatgtt tattgttttt taaagaatgt ttggcactct ctaacgtcca
     2221 ctagcttact gagttatcag gtgcaggtca gactcttggc tacagtgaga ggcagcttct
     2281 agacagagtt gcttaatgaa agggtttgta atactttaca aaccattacc tgtacctggc
     2341 ctggcctcca aaatattaac attctttttc tgttgaaact cgcgagtgta actttcatac
     2401 cacttgaatt tattgatatt taattatgaa aactagcatt acattattaa acgatttcta
     2461 aaatcaaaac atacttaatc tgataccaag gaagggaggg agtggttata agcaaatgaa
     2521 aacaaatttt gagagacaga gcaaaagtaa aatcattcta tagaaaaggt gtgtttattt
     2581 cttaactctt gagttctttt aaaattagaa cctaaatgat gcagtcaggg gtatgaccaa
     2641 ttccacatga gtgtcacctg tacattttat taccactacc tccagtttcc aaggcaggga
     2701 agaagagggg aatagtcaaa gcaatatgac tagctaggtt tgggctgctg tttgggctgc
     2761 tgtttttcct ggacttttat gccaaactac ataaatactt cttggaattc ctgatttacg
     2821 ctcaactttg atccctggac cctagggcca ttagtttcct tgaagcagtc ctgctggtgg
     2881 atgagcaggg aaacttgtag accctgggaa gccaattggt agagggtgcc tgcccgcccc
     2941 atcagactgt cccaactggg gtgggggtga ggatcatagt gatttctttt taaaagctag
     3001 tatgactagt gagaatggat gtccaagggc ttttctcttc ctcccccgag tccacgattc
     3061 ttcatttgtg atcagggttg gggtaacttc accttggtga tcatatattc tttttacaaa
     3121 ccaatccagc caagatatat gtgcttttct aaacagcttg taaaagcaaa aacacaatgt
     3181 atacacatat aaaactgatt tttttttttt tgcttatata ctttgctcag gtggagaaca
     3241 aggtatgaaa gccattatat ggtgtccctt ggggaccatc ccataagtcc agggtgttcc
     3301 caatataccc acaagacaac atgttcaaaa cttcatcacc tggtccccac aaacctacca
     3361 ccctcttcca ttccctcact ctggtaaaag caagccatct tctccagtta cgcaaggcag
     3421 aagcccagga gtgagcacaa tgctgccctt tcccttgctt ctgacttctc atcaatccta
     3481 ttagaactac tgactgcacc ccacctctcc tccatcccca cgatcactgc cttcatatga
     3541 actgcctttc ctgtcctctg gactttcagt ggcttccttt tttgctcacc tgtccacttt
     3601 cttccccttc cctctctcca tccctccaca tagtcatcag aaagatcatc aaaacaggca
     3661 aatgtgactg tattatttcc ctcacttatc tttgtatgca gtttagagtg atttttaaga
     3721 gcacacactt tgaaattagg agaacctgtg tctgaatcct gatttttgcc atttgtgtga
     3781 cttgtcaggt tatttaatct ctttgagcct gtccctcctc agtgaaatag gaataataat
     3841 acctacctca tagagttgtt gtgaggattc agtgagatag actgtttcta aagtgtgtag
     3901 cacagtgcct ggcacatagt gggtgctcac attggagcac actattgtcg ctatccctgc
     3961 aggtgtgagt tttacatcct ttccaagagg catttgctaa taaccctaac taccgcctat
     4021 ccctgtgaca ctattttctg cctcagcacc ctatttcttt tgtagacatt ttctaagtct
     4081 gtattttatt tatttgttgg ctttttgtct atttctctac tagaatgtaa gctccatgag
     4141 agcaaggatg tgtttgtctg atttcccgtt acagccccag cacttagctg agtgactggc
     4201 acctagtaag cactcagtaa ctgtttgctg aataaatgga agaatggtca aaaggctctt
     4261 cttgatctga tccctgcttg tctgtctggc ctcatgctgc cactattcct cactgcacct
     4321 tcagctcaag aaaaacctct ttacttgctt ctttgaagtg ccccttgggc ctctgtgtct
     4381 ttgtacctgt tgttcccttt gcctgaaaca ccctttgcct gcccgtctac atggtgaatt
     4441 cctaatcatt gatgaagtct cagctcaatg gctacctcct ctgtgaagct gtccctgatt
     4501 tcttcaggca gatgttcttt cccgccttcc ctgttcccac cgcactttgt aaatacctcc
     4561 atggaagcac tttattgtat tgtattgtaa tttttttttg catgtctgtt tctactatta
     4621 gactgtaagc accgtaaaag cagagattgt gctttttcat cctcatgttc cccgagccta
     4681 gcccagtgcc tggcacgtaa tagcttgtca ttaaatattt gttgaatgaa tctgtgaatg
     4741 aacaagagtc atgcagttgg aaaagtgtta gaagctgtat gtattataaa ggaaagaaat
     4801 gcccttggtg gcttccaggg tgtttttttt tttttttttg gtctaaagag gagatctaga
     4861 gagtgttata caatctcttt gaatataaga ttactcgttt ggatttttaa aataatatag
     4921 atcttcataa ataattgcaa agatataaac aagctttgtg atttctcaaa tgctatgaag
     4981 tccaaaataa atatttgcac tattagtatt tccatagtaa atgctatgag aaaatgtaca
     5041 gcaataaatt ttgaagcttt aaaa
//
