LOCUS       NM_020746              11731 bp    mRNA    linear   PRI 27-MAY-2024
DEFINITION  Homo sapiens mitochondrial antiviral signaling protein (MAVS),
            transcript variant 1, mRNA; nuclear gene for mitochondrial product.
ACCESSION   NM_020746 NM_024030 XM_045472
VERSION     NM_020746.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 11731)
  AUTHORS   Chen,J., Wang,Q., Zhong,B., Zheng,H., Wang,D., Huang,X., Liu,L. and
            Liu,T.
  TITLE     Activation of the RIG-I/MAVS Signaling Pathway during Human
            Adenovirus Type 3 Infection Impairs the Pro-Inflammatory Response
            Induced by Secondary Infection with Staphylococcus aureus
  JOURNAL   Int J Mol Sci 25 (8), 4178 (2024)
   PUBMED   38673764
  REMARK    GeneRIF: Activation of the RIG-I/MAVS Signaling Pathway during
            Human Adenovirus Type 3 Infection Impairs the Pro-Inflammatory
            Response Induced by Secondary Infection with Staphylococcus aureus.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11731)
  AUTHORS   Huang,C., Yin,Y., Pan,P., Huang,Y., Chen,S., Chen,J., Wang,J.,
            Xu,G., Tao,X., Xiao,X., Li,J., Yang,J., Jin,Z., Li,B., Tong,Z.,
            Du,W., Liu,L. and Liu,Z.
  TITLE     The Interaction between SARS-CoV-2 Nucleocapsid Protein and UBC9
            Inhibits MAVS Ubiquitination by Enhancing Its SUMOylation
  JOURNAL   Viruses 15 (12), 2304 (2023)
   PUBMED   38140545
  REMARK    GeneRIF: The Interaction between SARS-CoV-2 Nucleocapsid Protein
            and UBC9 Inhibits MAVS Ubiquitination by Enhancing Its SUMOylation.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 11731)
  AUTHORS   Shi,T.T., Huang,Y., Li,Y., Dai,X.L., He,Y.H., Ding,J.C., Ran,T.,
            Shi,Y., Yuan,Q., Li,W.J. and Liu,W.
  TITLE     MAVI1, an endoplasmic reticulum-localized microprotein, suppresses
            antiviral innate immune response by targeting MAVS on mitochondrion
  JOURNAL   Sci Adv 9 (35), eadg7053 (2023)
   PUBMED   37656786
REFERENCE   4  (bases 1 to 11731)
  AUTHORS   Noval,M.G., Spector,S.N., Bartnicki,E., Izzo,F., Narula,N.,
            Yeung,S.T., Damani-Yokota,P., Dewan,M.Z., Mezzano,V.,
            Rodriguez-Rodriguez,B.A., Loomis,C., Khanna,K.M. and
            Stapleford,K.A.
  TITLE     MAVS signaling is required for preventing persistent chikungunya
            heart infection and chronic vascular tissue inflammation
  JOURNAL   Nat Commun 14 (1), 4668 (2023)
   PUBMED   37537212
  REMARK    GeneRIF: MAVS signaling is required for preventing persistent
            chikungunya heart infection and chronic vascular tissue
            inflammation.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 11731)
  AUTHORS   Yi,J., Wang,L., Hu,G.S., Zhang,Y.Y., Du,J., Ding,J.C., Ji,X.,
            Shen,H.F., Huang,H.H., Ye,F. and Liu,W.
  TITLE     CircPVT1 promotes ER-positive breast tumorigenesis and drug
            resistance by targeting ESR1 and MAVS
  JOURNAL   EMBO J 42 (10), e112408 (2023)
   PUBMED   37009655
  REMARK    GeneRIF: CircPVT1 promotes ER-positive breast tumorigenesis and
            drug resistance by targeting ESR1 and MAVS.
REFERENCE   6  (bases 1 to 11731)
  AUTHORS   Meylan,E., Curran,J., Hofmann,K., Moradpour,D., Binder,M.,
            Bartenschlager,R. and Tschopp,J.
  TITLE     Cardif is an adaptor protein in the RIG-I antiviral pathway and is
            targeted by hepatitis C virus
  JOURNAL   Nature 437 (7062), 1167-1172 (2005)
   PUBMED   16177806
  REMARK    GeneRIF: KIAA1271 (aka Cardif, MAVS, VISA, IPS-1) has been shown to
            be targeted by hepatitis c virus (HCV) NS3/4A protease. This
            cleavage interferes with the Rig-I mediated dsRNA sensing pathway
            and therefore with the activation of IRF-3.
REFERENCE   7  (bases 1 to 11731)
  AUTHORS   Kawai,T., Takahashi,K., Sato,S., Coban,C., Kumar,H., Kato,H.,
            Ishii,K.J., Takeuchi,O. and Akira,S.
  TITLE     IPS-1, an adaptor triggering RIG-I- and Mda5-mediated type I
            interferon induction
  JOURNAL   Nat Immunol 6 (10), 981-988 (2005)
   PUBMED   16127453
REFERENCE   8  (bases 1 to 11731)
  AUTHORS   Xu,L.G., Wang,Y.Y., Han,K.J., Li,L.Y., Zhai,Z. and Shu,H.B.
  TITLE     VISA is an adapter protein required for virus-triggered IFN-beta
            signaling
  JOURNAL   Mol Cell 19 (6), 727-740 (2005)
   PUBMED   16153868
  REMARK    GeneRIF: Data suggest that VISA is critically involved in both
            virus-triggered TLR3-independent and TLR3-mediated antiviral
            interferon signaling.
REFERENCE   9  (bases 1 to 11731)
  AUTHORS   Seth,R.B., Sun,L., Ea,C.K. and Chen,Z.J.
  TITLE     Identification and characterization of MAVS, a mitochondrial
            antiviral signaling protein that activates NF-kappaB and IRF 3
  JOURNAL   Cell 122 (5), 669-682 (2005)
   PUBMED   16125763
  REMARK    GeneRIF: Mitochondrial antiviral signaling (MAVS) mediates the
            activation of NF-kappaB and IRF 3 in response to viral infection,
            and transmembrane domain targets MAVS to the mitochondria,
            implicating a new role of mitochondria in innate immunity.
REFERENCE   10 (bases 1 to 11731)
  AUTHORS   Chaudhary,P.M., Eby,M.T., Jasmin,A., Kumar,A., Liu,L. and Hood,L.
  TITLE     Activation of the NF-kappaB pathway by caspase 8 and its homologs
  JOURNAL   Oncogene 19 (39), 4451-4460 (2000)
   PUBMED   11002417
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DB219666.1, BC044952.1,
            DQ167126.1 and AL353194.13.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_020746.4.
            
            Summary: This gene encodes an intermediary protein necessary in the
            virus-triggered beta interferon signaling pathways. It is required
            for activation of transcription factors which regulate expression
            of beta interferon and contributes to antiviral innate immunity.
            [provided by RefSeq, Jul 2020].
            
            Transcript Variant: This variant (1) encodes the longer isoform
            (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data because no single transcript was available
            for the full length of the gene. The extent of this transcript is
            supported by transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AB033097.1, SRR1803613.170975.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            coronavirus related                :: involved in immune response
                                                  or antiviral activity
            gene product(s) localized to mito. :: PMID: 16125763; reported by
                                                  MitoCarta
            MANE Ensembl match                 :: ENST00000428216.4/
                                                  ENSP00000401980.2
            RefSeq Select criteria             :: based on conservation,
                                                  expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-418               DB219666.1         36-453
            419-725             BC044952.1         388-694
            726-1760            DQ167126.1         589-1623
            1761-2286           BC044952.1         1730-2255
            2287-11731          AL353194.13        5540-14984
FEATURES             Location/Qualifiers
     source          1..11731
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="20"
                     /map="20p13"
     gene            1..11731
                     /gene="MAVS"
                     /gene_synonym="CARDIF; IPS-1; IPS1; VISA"
                     /note="mitochondrial antiviral signaling protein"
                     /db_xref="GeneID:57506"
                     /db_xref="HGNC:HGNC:29233"
                     /db_xref="MIM:609676"
     exon            1..70
                     /gene="MAVS"
                     /gene_synonym="CARDIF; IPS-1; IPS1; VISA"
                     /inference="alignment:Splign:2.1.0"
     exon            71..254
                     /gene="MAVS"
                     /gene_synonym="CARDIF; IPS-1; IPS1; VISA"
                     /inference="alignment:Splign:2.1.0"
     CDS             138..1760
                     /gene="MAVS"
                     /gene_synonym="CARDIF; IPS-1; IPS1; VISA"
                     /note="isoform 1 is encoded by transcript variant 1; IFN-B
                     promoter stimulator 1; CARD adaptor inducing IFN-beta;
                     virus-induced signaling adaptor; virus-induced-signaling
                     adapter; CARD adapter inducing interferon beta; putative
                     NF-kappa-B-activating protein 031N; interferon beta
                     promoter stimulator protein 1"
                     /codon_start=1
                     /product="mitochondrial antiviral-signaling protein
                     isoform 1"
                     /protein_id="NP_065797.2"
                     /db_xref="CCDS:CCDS33437.1"
                     /db_xref="GeneID:57506"
                     /db_xref="HGNC:HGNC:29233"
                     /db_xref="MIM:609676"
                     /translation="MPFAEDKTYKYICRNFSNFCNVDVVEILPYLPCLTARDQDRLRA
                     TCTLSGNRDTLWHLFNTLQRRPGWVEYFIAALRGCELVDLADEVASVYQSYQPRTSDR
                     PPDPLEPPSLPAERPGPPTPAAAHSIPYNSCREKEPSYPMPVQETQAPESPGENSEQA
                     LQTLSPRAIPRNPDGGPLESSSDLAALSPLTSSGHQEQDTELGSTHTAGATSSLTPSR
                     GPVSPSVSFQPLARSTPRASRLPGPTGSVVSTGTSFSSSSPGLASAGAAEGKQGAESD
                     QAEPIICSSGAEAPANSLPSKVPTTLMPVNTVALKVPANPASVSTVPSKLPTSSKPPG
                     AVPSNALTNPAPSKLPINSTRAGMVPSKVPTSMVLTKVSASTVPTDGSSRNEETPAAP
                     TPAGATGGSSAWLDSSSENRGLGSELSKPGVLASQVDSPFSGCFEDLAISASTSLGMG
                     PCHGPEENEYKSEGTFGIHVAENPSIQLLEGNPGPPADPDGGPRPQADRKFQEREVPC
                     HRPSPGALWLQVAVTGVLVVTLLVVLYRRRLH"
     exon            255..429
                     /gene="MAVS"
                     /gene_synonym="CARDIF; IPS-1; IPS1; VISA"
                     /inference="alignment:Splign:2.1.0"
     exon            430..602
                     /gene="MAVS"
                     /gene_synonym="CARDIF; IPS-1; IPS1; VISA"
                     /inference="alignment:Splign:2.1.0"
     exon            603..762
                     /gene="MAVS"
                     /gene_synonym="CARDIF; IPS-1; IPS1; VISA"
                     /inference="alignment:Splign:2.1.0"
     exon            763..1295
                     /gene="MAVS"
                     /gene_synonym="CARDIF; IPS-1; IPS1; VISA"
                     /inference="alignment:Splign:2.1.0"
     exon            1296..11731
                     /gene="MAVS"
                     /gene_synonym="CARDIF; IPS-1; IPS1; VISA"
                     /inference="alignment:Splign:2.1.0"
     regulatory      9993..9998
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MAVS"
                     /gene_synonym="CARDIF; IPS-1; IPS1; VISA"
                     /note="hexamer: AATAAA"
     polyA_site      10014
                     /gene="MAVS"
                     /gene_synonym="CARDIF; IPS-1; IPS1; VISA"
                     /note="major polyA site"
     regulatory      11038..11043
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MAVS"
                     /gene_synonym="CARDIF; IPS-1; IPS1; VISA"
                     /note="hexamer: AATAAA"
     polyA_site      11065
                     /gene="MAVS"
                     /gene_synonym="CARDIF; IPS-1; IPS1; VISA"
                     /note="major polyA site"
     regulatory      11704..11709
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MAVS"
                     /gene_synonym="CARDIF; IPS-1; IPS1; VISA"
                     /note="hexamer: AATAAA"
     polyA_site      11731
                     /gene="MAVS"
                     /gene_synonym="CARDIF; IPS-1; IPS1; VISA"
ORIGIN      
        1 ctcctcgctg cgggaagggt cctgggcccc gggcggcggt cgccaggtct cagggccggg
       61 ggtacccgag tctcgtttcc tctcagtcca tccacccttc atggggccag agccctctct
      121 ccagaatctg agcagcaatg ccgtttgctg aagacaagac ctataagtat atctgccgca
      181 atttcagcaa tttttgcaat gtggatgttg tagagattct gccttacctg ccctgcctca
      241 cagcaagaga ccaggatcga ctgcgggcca cctgcacact ctcagggaac cgggacaccc
      301 tctggcatct cttcaatacc cttcagcggc ggcccggctg ggtggagtac ttcattgcgg
      361 cactgagggg ctgtgagcta gttgatctcg cggacgaagt ggcctctgtc taccagagct
      421 accagcctcg gacctcggac cgtcccccag acccactgga gccaccgtca cttcctgctg
      481 agaggccagg gccccccaca cctgctgcgg cccacagcat cccctacaac agctgcagag
      541 agaaggagcc aagttacccc atgcctgtcc aggagaccca ggcgccagag tccccaggag
      601 agaattcaga gcaagccctg cagacgctca gccccagagc catcccaagg aatccagatg
      661 gtggccccct ggagtcctcc tctgacctgg cagccctcag ccctctgacc tccagcgggc
      721 atcaggagca ggacacagaa ctgggcagta cccacacagc aggtgcgacc tccagcctca
      781 caccatcccg tgggcctgtg tctccatctg tctccttcca gcccctggcc cgttccaccc
      841 ccagggcaag ccgcttgcct ggacccacag ggtcagttgt atctactggc acctccttct
      901 cctcctcatc ccctggcttg gcctctgcag gggctgcaga gggtaaacag ggtgcagaga
      961 gtgaccaggc cgagcctatc atctgctcca gtggggcaga ggcacctgcc aactctctgc
     1021 cctccaaagt gcctaccacc ttgatgcctg tgaacacagt ggccctgaaa gtgcctgcca
     1081 acccagcatc tgtcagcaca gtgccctcca agttgccaac tagctcaaag ccccctggtg
     1141 cagtgccttc taatgcgctc accaatccag caccatccaa attgcccatc aactcaaccc
     1201 gtgctggcat ggtgccatcc aaagtgccta ctagcatggt gctcaccaag gtgtctgcca
     1261 gcacagtccc cactgacggg agcagcagaa atgaggagac cccagcagct ccaacacccg
     1321 ccggcgccac tggaggcagc tcagcctggc tagacagcag ctctgagaat aggggccttg
     1381 ggtcggagct gagtaagcct ggcgtgctgg catcccaggt agacagcccg ttctcgggct
     1441 gcttcgagga tcttgccatc agtgccagca cctccttggg catggggccc tgccatggcc
     1501 cagaggagaa tgagtataag tccgagggca cctttgggat ccacgtggct gagaacccca
     1561 gcatccagct cctggagggc aaccctgggc cacctgcgga cccggatggc ggccccaggc
     1621 cacaagccga ccggaagttc caggagaggg aggtgccatg ccacaggccc tcacctgggg
     1681 ctctgtggct ccaggtggct gtgacagggg tgctggtagt cacactcctg gtggtgctgt
     1741 accggcggcg tctgcactag tgaagccctg ggctcttccc accacccatc tgttccgttc
     1801 ctgcagtaca cctggcccct ctccgaagcc ccttgtccct ttcttgggga ttgtggaggc
     1861 tgggtcagag gggagttaag ggactgcagg cctggcagca ggacatgcct tggctgaacc
     1921 aagtcctgag agcagcatct ctgtccccac ggtgccttgt gtgggtcccc gtccttggct
     1981 ttctgggtcc tgggctgccc ccagtgctcc agaccttccc cactggcaat ccaggttatc
     2041 atccatgtcc tccagaggag cttcctcctc caggcctcag ccctgttggc ccaggtggag
     2101 caggagggac cactggaaca tgtggtgctt gggaatgcct ctcctgttgc attggtccct
     2161 gaaggcctca gggcaggtat gtggtgtgtg ggcgactcca caagacctgc ctcccatcct
     2221 ggcagcccag cctgagaccg ttgcattgag gcaggcagga gcggcagggt ggctgctctc
     2281 caggagccca actgccttga gttcctgccc cactgggccc cctcccctgc tgggcaatcc
     2341 tgggaaggtc tggaggttcc tgtggacctc agggaagcca ggggcagctg tcaggcctga
     2401 ggaagacctg tggagctcct ctccagcctc ctctttccct cccctctggt ctccattctc
     2461 ttcagctccc tacatgggct ggggaggaga cacctggtgg gcagagctca ggcagaggtt
     2521 tggatttcag ctccctcact tccggggctg tgtggctttg gcagatgtca gacttctggt
     2581 cttgcttctc cacgtggaca gtgagtatct ggctcattct tcactgggtt cttctgagat
     2641 tgaacctaca ggtgtttgcc aagtgcctgg cccagagcaa gtggccactg cttctcccat
     2701 ctctctcctg cccaacctgg tagagctgag ggcatgagag gcagagtgca cagtggtcaa
     2761 gggtgcagct ctgcagcaca ggcagcctag gcctgcgtcc caacctgcct ctcaccagct
     2821 ctgtgacctt gggcaaggga tttatctgtc tgtcccttag ttttctcacc tgtaaaagga
     2881 ggataagtat atatatatat ttcccagtgt tgtgaagatt aaaggagttt atcgatgtag
     2941 gtcttaggat gagtcctggc atttaccaag ggttggatat atgttattat cactattaag
     3001 tgttgagggt ccaggcatgc tgggcaacag ggaccccatc tctacaaaaa agtttaaaaa
     3061 attagccagg cgtggtggtg cacctgtcgt cttagctact tgggaggctg aggtgggagg
     3121 atcacttgag cccagaagct tgaagctgca gtgagctagg atcgtgccac tgcactccaa
     3181 cctgggtgag agagcgagac cctgtctcaa gaaaaagaaa aatgcagaga aacaggagtc
     3241 ttggctactc ctttagaggc agactcagac cctcctgcct cacagcttta tctttgtatt
     3301 tgccccttac tttatcttgt gccttgagaa attgctgggg agagaggtat gtccactggg
     3361 cagctgtaca ggatggagga tatagggcgt ttccactccc agcagccagg ttccctcacc
     3421 ccaagctcac ccactgttgg ggagattatc tacaataaca ccagaaacac attggggtgg
     3481 attgggggta tccttatggg ttcttttcag ggaaccattg ctggacaagg cacaggagcc
     3541 acctccattt ctgagctctg caagggacaa gaactagagc catcaggggc tgggctcact
     3601 gtggccccac cccaagccgt cagcctccag ggatctacac cctgccttgg ctgctacagc
     3661 tttttcactc cactgcccta ggggagttca gcaacctaat gatctctatc tctgaacatc
     3721 tcttcatccc atgctccaag tccagcaacc tgcaccctgg aaccaggagt ggaccctacc
     3781 cgagctgtct gtattaatcc ccatccccca ccaccaatct taaaaagccc tctgtccccc
     3841 taccctaaac cccagttagg tacccatgct gggcaggtca gttaacaatt tatgcacagg
     3901 tactagtttt attgtattac cgttccaggg tagctttgaa aaaagtatct caaaaaggca
     3961 acatgggccg agcgcagtgg ctcacgcctg taatcccagc actttgggag gccaaggtgg
     4021 gcagatcgcc tgaggtctgg agttcaagac cagcctggcc aacagggtga aaccccgtct
     4081 ctacaaaaat aagaaaatta gccaggtgta gtggcagacg tctgtaatcc cagctattca
     4141 ggaggctgag gcacgagaat tccatgaacc caggatgcgg aggttgcagt gagccgagat
     4201 tgtgccactg cgctccagcc tgggcgacag agtggtattc tgtttcaaaa aaaaaaaaaa
     4261 aggcagtatg tagccccgaa gactgttgcc caagtggtag aatgttagca cactaccagc
     4321 ctaggtaaaa aatacaaaaa gtaactgggc atggcggcgc ccatctatag tcccagctac
     4381 atgggaggct gaggtgggaa gataagtcac ttgagcccgc caggaggcgg aggttgtagt
     4441 gagctgagat cgcaccactg cactccagcc tgggtgaccg agtgatactc tgtctcaaag
     4501 aaaaaaaatt ataattttag cacagtaacc agccatgatg ggagataccc tgggtaaggc
     4561 atgtagaaag ggttgaggga ccttcccagt cccctagccc cgcctcccat cctcccatct
     4621 ttttcttttt tctttttttt agagaatcac ccagcctgga gcgaagtggt gcaatcataa
     4681 ctcactgtat ccttaaactc ccgggcttaa gcgatcctcc tgcctcagcc ttctgagtaa
     4741 ctaggacttc aggtacctgt caccatgcct ggctaattaa attttttttt cttttttttt
     4801 tttgagatgg agtcttgctc tgtcacccag gctggagtgc agtggcgcga tctcagctca
     4861 ctgcgacctc cacctcctgg gttcaggcca ttctcccgcc tcagcctcca gagtagctgg
     4921 gactacaggc gcctgccacc acgcctggct aatttttttg cacttttagt agagacgggg
     4981 tttcactgtg ttagccagga tggtctcgat ctcctgacct tgtgatccgc ccgcctcggc
     5041 ctcccaaagt gctgggatta caggcgtgag ccaccgcgcc cagccaaatt aaatttttta
     5101 tagagatgag gtcatgctgt tatgttgccc aggttggcct catgagatct tgccttagcc
     5161 tcccaaagtg ctgggattac agatgtgaga cactgcaccc aaaccccacc actttttttt
     5221 ttcctttttc tttttttgag acagtcttac tccgttgccc aggctggagt gtagtggcat
     5281 gatctcagct cactgcaacc tccgcctccc gggttcaagc aattctcctg cctcagcctc
     5341 ccgagtagct gggattacag aggcctgcca ccacacccga ctaattttcg tatttttagt
     5401 agagacgggg tttctccatg ttggccaggc tgttcttgaa ctcctgacct caagtgctcc
     5461 acctgcgttg gcttcccaaa gtgctgggat acaggagtga gccactgcgc ctggctgatc
     5521 ccagcacttt tcaaatgatg ccgctcaaag ccgtgacttg gcctactttg aacagcaaac
     5581 ttgttgctgc tgttgtcaac ctgaaggcct ctcaaatgcc agcttcaagc agggtgtgaa
     5641 ttggccagtg tcagatctca ggagtcctgt gttgagagtg tggctttcag ctgcggggag
     5701 ctgcacttgg tggggaaagc caggcaggtc accctcacag ccagataatg tggaggtcag
     5761 aacccaagga agggagtgag acctccactc ccagtggggg acctggccac ccatccttgg
     5821 ggacctgaga aagcgtactt caccttgggg tgaaggctgg gtggggccag agggaccagt
     5881 gccctcctca gtgcttaggg gcagagccac ctgcagcaat ggtatctgca tattagcccc
     5941 tctccacctt ctttctcccg ctgaatcatt tccctcaaag cccaagagct gtcactgctt
     6001 ctttctccct gggaagaatg cgtggactct gcctggtgat agactgaagc cagaacagtg
     6061 ccacaccctc gccttaattc cttgctaggt gttctcagat ttatgagact tcttagtcaa
     6121 atatgaggga ggttggatgt ggtggcttgt gcctgtaatc ccagcatttt gggaagccga
     6181 ggtgggagga tcccttgaag ccaggagttt gagacaagcc tgggcaacaa agcaagaccc
     6241 tatctctaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaat ctaggagatg
     6301 ctctttaccc tgcctggcct caaactatta atagcttcct ttgagcaaca ttatttatta
     6361 tgaactttca aacacaaaaa agtagagaga gtagaataac aaatccccat gagcccatca
     6421 cccaacttca gtaattatca attcatggcc atcttgttca cccctgcctg cttccctgct
     6481 tcccctcatt ctgcagaggt tcttttcttt tgagacagag tgttgctctg ttgcccaggc
     6541 tggagtgcag tggtgcaact tcggctcact gcaacctccg cctcccaggt tcaagtgatt
     6601 ctcctgcttc agcctctcaa gtagctggga ttacagatgc ccgccaccac acctggctaa
     6661 ttttcgtatt tttgttagag atggggtttc accatgttgg ccaggctggt ctcgaactcc
     6721 tgacctcaag tgatccgccc gccttggcct cccaaaatgc tgggattaca ggtgtgaacc
     6781 acggtgcctg gccactgtac aggttattta tagaagttgg agagtgaagg gttgagaaag
     6841 ccaaggggca gatgcgggtc tggaggattt tgtgcctaag gccctctctt tgctcccaga
     6901 cagcatgaag taacaatgag gcatccacct cttggttttg tggcctctgt ggatgacgtc
     6961 tctcaccttg aaccagttca gagttggagt agcgcaggat cctgtcttca gaggaggggc
     7021 cgaagcgggt tcctctgttg tcaagctctt tggaggtgcc tggctgctac tactgtccca
     7081 gagaggtgat gatgaatgat gggtgtgtcc agtggcagtt tgccccactg aggcaggggc
     7141 ttccactagg ccctgacaga gcccttccag caggcagaaa tccctgtgct aggcaagatt
     7201 caaactccgt agcatgtctc ctgctcccat ctcttaggaa tggagtcctt caggccttga
     7261 gtcccacatt ttccatgatg ctccattaag cagctgatag cacccccacc tccagggaaa
     7321 gtgagttcag agtccttggt ctaatgcatc tgtgttgaaa ttgaggcctt cccctgtgtt
     7381 cacctttctg ctctttttct tttagcccaa ggctatgaag gcctcattcg gtgctgggca
     7441 tggtcactcc tagcattcct cactctgttg ctaacagcaa cagcaataat aataagggtt
     7501 acaacttact ccatacctta ctgtctgcca ggcattaagc taagtgcttt acatatatta
     7561 agtcatttaa tcctcataat gaccctatga aagagatacc atctcaaccc aattgacagc
     7621 tggtttgcaa gattaggagg gatgaaggac ccaggggaca atgcgaggga aaactctgac
     7681 cccggggccc caggctggat gttctttatg cctgtgaacc acagcttatc acatgtctgg
     7741 agttagggac cccacttaaa gtgagatttt ggctggaggt ggtggatcat acctataatc
     7801 ccagcacttt gggagaccaa ggcagaagga ctgcttgagg ccaggagttc aaaaccagtg
     7861 taggtaacag ctagacccta tctctacaaa aaatttaaaa attagctggg tgtggtggta
     7921 tgtgcctcaa gttccagcta ctcaggaggc tgaggtggga ggatcacttg agcacaggag
     7981 tttgaagtta cagtgagcta tgatggcacc actgcacttc agcctaggca acagagggag
     8041 accctgtctt taaagtacat agaggttttt cacaccaaca catctctgcc cagtgtgcca
     8101 acatctgcca cctgctataa tagtactata acactcaata tgtaattaat gtagtctcag
     8161 ggatgttatg acaatatgat tacaactatc acgtgtgtgc ccagccaggc tcaatgcccc
     8221 aggctgggcg aggtggggca ggggacacag cctaaaatgc caggcctcag gaagccattt
     8281 ggtttagcag acattgttta ttaaaggagt tacctatgcc agatcgaagg cctaagatga
     8341 ttaagacact atgagtgcct tcaagtggtt ggggacgttc atgattgtgg tacagacaaa
     8401 taggctttca catcattctt ttatgtaatc atacaacaga tatttgcacc tacatgtgca
     8461 gagcactgtg ataggcctca gtgacacaga ataatacggc aaagacccca cccgatgagc
     8521 cccctcccac cacccaccag tacagtaggg ggtggtttaa tggagtgttc ctggaatatg
     8581 aagtgggggc aggcattagg ggtggcaaag ggacaagtgt ttatctgatc agttatgtac
     8641 tgtttataat aagtaaatca gcagaggggg aataatactt agaacctata gagagtaaat
     8701 ctgacaagat gaaatgctga tgaaaatatg gaggaaatga aactctcatg ggttttgcag
     8761 ggaatctaag tcagtgctgt gttgtgaatg taggtgtacc ctttgaattc atatgttgaa
     8821 tcctaacccc caaagcaatg gcattaagag gtggggcctt tggggctggg tatggtggct
     8881 catgactgta atcccagcac tttgggatgc tggcaggggg cagatcactt gaagccagga
     8941 gtctgagatc agcctggcca acatggtgaa accccatctg tactaaaaat acaaaaatta
     9001 gccaggtgtg atggcgtaca tctgtaattt cagccactcg ggaggctgag acaggagaat
     9061 agcttgaacc cagtaggtgg agatttcagt gagccgagat cgtgccactg cactccagcc
     9121 tgggtgacag agcgagactc catctcaaaa aaataataaa gatgtggggc ctgtgggagg
     9181 tggttaggtc atgagggtgg agatcatgaa tggggttagc accttataaa acaggcttga
     9241 gggagccctt ctgtcccttc taccatgtgt ggatgcagtg agaaggcacc gtatctctga
     9301 agcagagagc ccgccctgga cactggatct gctggcacct tgatcttgga cttcccagcc
     9361 tctagaactg tgagaaataa ttttttgttg tttacaaatt acccaggcta aggtgtttca
     9421 ttgtaacctg aatggaccaa gctggtgtga ccctgttgga aaactggcag tatctaccaa
     9481 aagccgaaca tacgtataaa ctgatccagc agttccactc ctgggtatgt acaccacaga
     9541 aagctatgtc caccgagaca ttggcaagaa tgtttctaac cacacgctga ctgtagcccc
     9601 aaacctgaaa caacccaaat gtccatccac caacccaaat gtccatccac agttgaagct
     9661 acagtgaagt cacagggtcg aatactactg cacagcaacg aatatgaatg aaaatatcgc
     9721 tatgcacagc aacatggata aatttcacag acatgaggtc aagcaaaaga ggtcagagtc
     9781 ctcatcatca agagagaatt cattgtatga ttctcttcct acaaaaagta cagaaataag
     9841 caaaactgat ccatggtgtt agaagccagg ggaacagtta acaggggagg gatactgggg
     9901 aggggcatcc tggagtgctg gtctacctca tctgggtgtt gatttcacga gtattgtcag
     9961 tttgtttcca gactccctgt tggagatgtg gaaataaaaa ccacctaaac aagagcagag
    10021 aggccatttg gtcaaagttt gcaaaggagt cagccatgat tgcttgtatt tggcaggggt
    10081 caaaggcagg cagggactgt gaaatgttat agtggaaaaa aagggaaggc tctgggtgtg
    10141 ctgtgattgg agattgttgg catggggaca gagcggacta actggagggg catctttggt
    10201 tggttggggg ggtatatttg gctttctctg gttggtctgg agttggaaga gggggtgtgg
    10261 tggctgggga ttgggaagaa gctggcagcc actaagttca gactgttctg ggtccgattg
    10321 ctgctgaggc tgtggtttgg cttccttggc ttcccaggct ggtcatgggt ttctggccag
    10381 agtctattgt catatgtggc ctggccattg tccagttgta tgttcagtct cttggaagga
    10441 agggtattga ctctgagagg ggccaccatc gctggaatgg gggacacaca gtacttcctc
    10501 cagctgccta caccccccta gggtcagtgg cgcctgcctg tgagggtgag cccaatggct
    10561 agagggctct gctccaagtc attgcttact acacccacaa acattcttcg ttctttaagg
    10621 cctaacttaa agcccagatc ctacaggaaa ccttgattag acccctctct ttattaagct
    10681 tcctaagatc aaaccctgct tttgtgtaaa tgctgacctc cttgcctaca ttttaaaaac
    10741 ctagagctgg gcatgatggc cccagcctgt aatcccagtg attcaggaga ctgaggtggg
    10801 aggattgcta gaagccagga gttcgagacc agcctgggta acatagctag accacatctc
    10861 ttaaaataaa atagttaatt tagccaggca tgatgatata tgcctgtagt cccaactact
    10921 tggaaggctg aggtgtgagg atctttgagc ccgggaggtc gaggctacag taagctatga
    10981 tctcaccact gtactccagc ctgggtgaca gagcgagacc cagactcaaa aaataaaaat
    11041 aaaaaccctg aatatcttcc ttctacttct tcagtgctgt ttttatttaa aaaaaaaaaa
    11101 aaccagccaa aaccacaact ttttactgaa gtgtaatgta aatgctgtaa aaggcagtga
    11161 aaggcacaag ggaggtggag gggtaggaag ggtggaagtg gcgggaggaa gtggcagggc
    11221 aggcaaaatg aagggaagcc ctgggttctt gtcctgcatc cgcagccagc tcccactttc
    11281 ctcaccctcc aggacctgta aactgtgagg ctggaccagt tatgtcaaat ctgtcctccc
    11341 ccagagctca gtccctctgc ccttgggtgt ccttggcaca aggcaggcta ggctgcacca
    11401 gcttcctcca tctccgtcct gcctccccca tccccaggtg ccattcccac accatctgaa
    11461 tcactgattt cctcgcaatc agacgctatc ttccagttaa tcacttcgct tgtatttaac
    11521 ataagaaaga aaaacccttt cattatcaca tacagctgga aatcggcttc ttgcaggagg
    11581 cgtatccaaa ggaattggag aagagataaa ctggtaattg gtgaaagaat tactttaatt
    11641 ttttttccta cttgctgtca tgatgatgtc cttagaattg tgagcccgtg gacacttctg
    11701 tacaataaat ctgctattat tacttctaga a
//
