LOCUS       NM_001164664           10677 bp    mRNA    linear   PRI 03-OCT-2024
DEFINITION  Homo sapiens microtubule associated serine/threonine kinase family
            member 4 (MAST4), transcript variant 3, mRNA.
ACCESSION   NM_001164664
VERSION     NM_001164664.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 10677)
  AUTHORS   Lee,D.J., Kim,P., Kim,H.Y., Park,J., Lee,S.J., An,H., Heo,J.S.,
            Lee,M.J., Ohshima,H., Mizuno,S., Takahashi,S., Jung,H.S. and
            Kim,S.J.
  TITLE     MAST4 regulates stem cell maintenance with DLX3 for epithelial
            development and amelogenesis
  JOURNAL   Exp Mol Med 56 (7), 1606-1619 (2024)
   PUBMED   38945953
  REMARK    GeneRIF: MAST4 regulates stem cell maintenance with DLX3 for
            epithelial development and amelogenesis.
REFERENCE   2  (bases 1 to 10677)
  AUTHORS   Fujiwara-Tani,R., Sasaki,T., Bhawal,U.K., Mori,S., Ogata,R.,
            Sasaki,R., Ikemoto,A., Kishi,S., Fujii,K., Ohmori,H., Sho,M. and
            Kuniyasu,H.
  TITLE     Nuclear MAST4 Suppresses FOXO3 through Interaction with AKT3 and
            Induces Chemoresistance in Pancreatic Ductal Carcinoma
  JOURNAL   Int J Mol Sci 25 (7), 4056 (2024)
   PUBMED   38612866
  REMARK    GeneRIF: Nuclear MAST4 Suppresses FOXO3 through Interaction with
            AKT3 and Induces Chemoresistance in Pancreatic Ductal Carcinoma.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 10677)
  AUTHORS   Cui,Y., Wang,F., Zhang,D., Huang,J., Yang,Y., Xu,J., Gao,Y.,
            Ding,H., Qu,Y., Zhang,W., Liu,W., Pan,L., Zhang,L., Liu,Z., Niu,T.,
            Liu,T. and Zheng,Y.
  TITLE     Estrogen-Responsive Gene MAST4 Regulates Myeloma Bone Disease
  JOURNAL   J Bone Miner Res 37 (4), 711-723 (2022)
   PUBMED   35064934
  REMARK    GeneRIF: Estrogen-Responsive Gene MAST4 Regulates Myeloma Bone
            Disease.
REFERENCE   4  (bases 1 to 10677)
  AUTHORS   Lee,S.J., Park,J., Lee,D.J., Otsu,K., Kim,P., Mizuno,S., Lee,M.J.,
            Kim,H.Y., Harada,H., Takahashi,S., Kim,S.J. and Jung,H.S.
  TITLE     Mast4 knockout shows the regulation of spermatogonial stem cell
            self-renewal via the FGF2/ERM pathway
  JOURNAL   Cell Death Differ 28 (5), 1441-1454 (2021)
   PUBMED   33219327
  REMARK    GeneRIF: Mast4 knockout shows the regulation of spermatogonial stem
            cell self-renewal via the FGF2/ERM pathway.
REFERENCE   5  (bases 1 to 10677)
  AUTHORS   Landoulsi,Z., Laatar,F., Noe,E., Mrabet,S., Ben Djebara,M.,
            Achaz,G., Nava,C., Baulac,S., Kacem,I., Gargouri-Berrechid,A.,
            Gouider,R. and Leguern,E.
  TITLE     Clinical and genetic study of Tunisian families with genetic
            generalized epilepsy: contribution of CACNA1H and MAST4 genes
  JOURNAL   Neurogenetics 19 (3), 165-178 (2018)
   PUBMED   29948376
  REMARK    GeneRIF: Molecular studies incriminated different genes, mainly
            CACNA1H and MAST4. Since at least 2 susceptibility genes were
            likely shared by different populations, genetic factors involved in
            the majority of Tunisian Genetic generalized epilepsies families
            remain to be discovered.
REFERENCE   6  (bases 1 to 10677)
  AUTHORS   Steffens,M., Leu,C., Ruppert,A.K., Zara,F., Striano,P.,
            Robbiano,A., Capovilla,G., Tinuper,P., Gambardella,A., Bianchi,A.,
            La Neve,A., Crichiutti,G., de Kovel,C.G., Kasteleijn-Nolst
            Trenite,D., de Haan,G.J., Lindhout,D., Gaus,V., Schmitz,B.,
            Janz,D., Weber,Y.G., Becker,F., Lerche,H., Steinhoff,B.J.,
            Kleefuss-Lie,A.A., Kunz,W.S., Surges,R., Elger,C.E., Muhle,H., von
            Spiczak,S., Ostertag,P., Helbig,I., Stephani,U., Moller,R.S.,
            Hjalgrim,H., Dibbens,L.M., Bellows,S., Oliver,K., Mullen,S.,
            Scheffer,I.E., Berkovic,S.F., Everett,K.V., Gardiner,M.R.,
            Marini,C., Guerrini,R., Lehesjoki,A.E., Siren,A., Guipponi,M.,
            Malafosse,A., Thomas,P., Nabbout,R., Baulac,S., Leguern,E.,
            Guerrero,R., Serratosa,J.M., Reif,P.S., Rosenow,F., Morzinger,M.,
            Feucht,M., Zimprich,F., Kapser,C., Schankin,C.J., Suls,A.,
            Smets,K., De Jonghe,P., Jordanova,A., Caglayan,H., Yapici,Z.,
            Yalcin,D.A., Baykan,B., Bebek,N., Ozbek,U., Gieger,C.,
            Wichmann,H.E., Balschun,T., Ellinghaus,D., Franke,A., Meesters,C.,
            Becker,T., Wienker,T.F., Hempelmann,A., Schulz,H., Ruschendorf,F.,
            Leber,M., Pauck,S.M., Trucks,H., Toliat,M.R., Nurnberg,P.,
            Avanzini,G., Koeleman,B.P. and Sander,T.
  CONSRTM   EPICURE Consortium; EMINet Consortium
  TITLE     Genome-wide association analysis of genetic generalized epilepsies
            implicates susceptibility loci at 1q43, 2p16.1, 2q22.3 and 17q21.32
  JOURNAL   Hum Mol Genet 21 (24), 5359-5372 (2012)
   PUBMED   22949513
REFERENCE   7  (bases 1 to 10677)
  AUTHORS   Martins-de-Souza,D., Guest,P.C., Mann,D.M., Roeber,S., Rahmoune,H.,
            Bauder,C., Kretzschmar,H., Volk,B., Baborie,A. and Bahn,S.
  TITLE     Proteomic analysis identifies dysfunction in cellular transport,
            energy, and protein metabolism in different brain regions of
            atypical frontotemporal lobar degeneration
  JOURNAL   J Proteome Res 11 (4), 2533-2543 (2012)
   PUBMED   22360420
  REMARK    GeneRIF: A protein encoded by this locus was found to be
            differentially expressed in postmortem brains from patients with
            atypical frontotemporal lobar degeneration.
REFERENCE   8  (bases 1 to 10677)
  AUTHORS   Shrestha,S., Irvin,M.R., Taylor,K.D., Wiener,H.W., Pajewski,N.M.,
            Haritunians,T., Delaney,J.A., Schambelan,M., Polak,J.F.,
            Arnett,D.K., Chen,Y.D. and Grunfeld,C.
  TITLE     A genome-wide association study of carotid atherosclerosis in
            HIV-infected men
  JOURNAL   AIDS 24 (4), 583-592 (2010)
   PUBMED   20009918
REFERENCE   9  (bases 1 to 10677)
  AUTHORS   Sun,L., Gu,S., Li,X., Sun,Y., Zheng,D., Yu,K., Ji,C., Tang,R.,
            Xie,Y. and Mao,Y.
  TITLE     [Identification of a novel human MAST4 gene, a new member of the
            microtubule associated serine-threonine kinase family]
  JOURNAL   Mol Biol (Mosk) 40 (5), 808-815 (2006)
   PUBMED   17086981
  REMARK    GeneRIF: high expression level of MAST4 in most normal human
            tissues, with an exception of in testis, small intestine, colon and
            peripheral blood leukocyte.
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC092373.2, AC016634.7,
            AC008872.6, AC008920.7 and AC044799.5.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Jun 2, 2019 this sequence version replaced NM_001164664.1.
            
            SUMMARY: This gene encodes a member of the microtubule-associated
            serine/threonine protein kinases. The proteins in this family
            contain a domain that gives the kinase the ability to determine its
            own scaffold to control the effects of their kinase activities.
            Alternative splicing results in multiple transcript variants
            encoding different isoforms. [provided by RefSeq, Mar 2014].
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            RNAseq introns :: single sample supports all introns SAMEA1968968,
                              SAMEA2155371 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000403625.7/ ENSP00000385727.1
            RefSeq Select criteria :: based on manual assertion, conservation
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-626               AC092373.2         7238-7863           c
            627-780             AC016634.7         131849-132002       c
            781-905             AC016634.7         102917-103041       c
            906-937             AC008872.6         113247-113278       c
            938-1026            AC008920.7         37721-37809
            1027-1096           AC008920.7         73479-73548
            1097-1175           AC008920.7         78914-78992
            1176-1333           AC008920.7         83752-83909
            1334-1409           AC008920.7         85853-85928
            1410-1619           AC008920.7         87683-87892
            1620-1721           AC008920.7         93415-93516
            1722-1854           AC008920.7         97404-97536
            1855-1922           AC008920.7         101999-102066
            1923-2008           AC008920.7         104334-104419
            2009-2217           AC008920.7         113527-113735
            2218-2356           AC008920.7         115130-115268
            2357-2489           AC008920.7         116831-116963
            2490-2655           AC008920.7         117840-118005
            2656-2757           AC008920.7         119880-119981
            2758-2880           AC044799.5         28671-28793         c
            2881-2993           AC044799.5         28375-28487         c
            2994-3121           AC044799.5         26112-26239         c
            3122-3357           AC044799.5         25529-25764         c
            3358-3558           AC044799.5         21319-21519         c
            3559-3788           AC044799.5         18042-18271         c
            3789-3911           AC044799.5         17328-17450         c
            3912-4048           AC044799.5         10316-10452         c
            4049-4230           AC044799.5         8120-8301           c
            4231-10677          AC044799.5         1315-7761           c
FEATURES             Location/Qualifiers
     source          1..10677
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="5"
                     /map="5q12.3"
     gene            1..10677
                     /gene="MAST4"
                     /note="microtubule associated serine/threonine kinase
                     family member 4"
                     /db_xref="GeneID:375449"
                     /db_xref="HGNC:HGNC:19037"
                     /db_xref="MIM:618002"
     exon            1..626
                     /gene="MAST4"
                     /inference="alignment:Splign:2.1.0"
     CDS             264..8135
                     /gene="MAST4"
                     /EC_number="2.7.11.1"
                     /note="isoform 3 is encoded by transcript variant 3;
                     microtubule-associated serine/threonine-protein kinase 4"
                     /codon_start=1
                     /product="microtubule-associated serine/threonine-protein
                     kinase 4 isoform 3"
                     /protein_id="NP_001158136.1"
                     /db_xref="CCDS:CCDS54861.1"
                     /db_xref="GeneID:375449"
                     /db_xref="HGNC:HGNC:19037"
                     /db_xref="MIM:618002"
                     /translation="MGEKVSEAPEPVPRGCSGHGSRTPASALVAASSPGASSAESSSG
                     SETLSEEGEPGGFSREHQPPPPPPLGGTLGARAPAAWAPASVLLERGVLALPPPLPGG
                     AVPPAPRGSSASQEEQDEELDHILSPPPMPFRKCSNPDVASGPGKSLKYKRQLSEDGR
                     QLRRGSLGGALTGRYLLPNPVAGQAWPASAETSNLVRMRSQALGQSAPSLTASLKELS
                     LPRRGSFCRTSNRKSLIGNGQSPALPRPHSPLSAHAGNSPQDSPRNFSPSASAHFSFA
                     RRTDGRRWSLASLPSSGYGTNTPSSTVSSSCSSQEKLHQLPYQPTPDELHFLSKHFCT
                     TESIATENRCRNTPMRPRSRSLSPGRSPACCDHEIIMMNHVYKERFPKATAQMEERLK
                     EIITSYSPDNVLPLADGVLSFTHHQIIELARDCLDKSHQGLITSRYFLELQHKLDKLL
                     QEAHDRSESGELAFIKQLVRKILIVIARPARLLECLEFDPEEFYYLLEAAEGHAKEGQ
                     GIKTDIPRYIISQLGLNKDPLEEMAHLGNYDSGTAETPETDESVSSSNASLKLRRKPR
                     ESDFETIKLISNGAYGAVYFVRHKESRQRFAMKKINKQNLILRNQIQQAFVERDILTF
                     AENPFVVSMYCSFETRRHLCMVMEYVEGGDCATLMKNMGPLPVDMARMYFAETVLALE
                     YLHNYGIVHRDLKPDNLLVTSMGHIKLTDFGLSKVGLMSMTTNLYEGHIEKDAREFLD
                     KQVCGTPEYIAPEVILRQGYGKPVDWWAMGIILYEFLVGCVPFFGDTPEELFGQVISD
                     EINWPEKDEAPPPDAQDLITLLLRQNPLERLGTGGAYEVKQHRFFRSLDWNSLLRQKA
                     EFIPQLESEDDTSYFDTRSEKYHHMETEEEDDTNDEDFNVEIRQFSSCSHRFSKVFSS
                     IDRITQNSAEEKEDSVDKTKSTTLPSTETLSWSSEYSEMQQLSTSNSSDTESNRHKLS
                     SGLLPKLAISTEGEQDEAASCPGDPHEEPGKPALPPEECAQEEPEVTTPASTISSSTL
                     SVGSFSEHLDQINGRSECVDSTDNSSKPSSEPASHMARQRLESTEKKKISGKVTKSLS
                     ASALSLMIPGDMFAVSPLGSPMSPHSLSSDPSSSRDSSPSRDSSAASASPHQPIVIHS
                     SGKNYGFTIRAIRVYVGDSDIYTVHHIVWNVEEGSPACQAGLKAGDLITHINGEPVHG
                     LVHTEVIELLLKSGNKVSITTTPFENTSIKTGPARRNSYKSRMVRRSKKSKKKESLER
                     RRSLFKKLAKQPSPLLHTSRSFSCLNRSLSSGESLPGSPTHSLSPRSPTPSYRSTPDF
                     PSGTNSSQSSSPSSSAPNSPAGSGHIRPSTLHGLAPKLGGQRYRSGRRKSAGNIPLSP
                     LARTPSPTPQPTSPQRSPSPLLGHSLGNSKIAQAFPSKMHSPPTIVRHIVRPKSAEPP
                     RSPLLKRVQSEEKLSPSYGSDKKHLCSRKHSLEVTQEEVQREQSQREAPLQSLDENVC
                     DVPPLSRARPVEQGCLKRPVSRKVGRQESVDDLDRDKLKAKVVVKKADGFPEKQESHQ
                     KSHGPGSDLENFALFKLEEREKKVYPKAVERSSTFENKASMQEAPPLGSLLKDALHKQ
                     ASVRASEGAMSDGRVPAEHRQGGGDFRRAPAPGTLQDGLCHSLDRGISGKGEGTEKSS
                     QAKELLRCEKLDSKLANIDYLRKKMSLEDKEDNLCPVLKPKMTAGSHECLPGNPVRPT
                     GGQQEPPPASESRAFVSSTHAAQMSAVSFVPLKALTGRVDSGTEKPGLVAPESPVRKS
                     PSEYKLEGRSVSCLKPIEGTLDIALLSGPQASKTELPSPESAQSPSPSGDVRASVPPV
                     LPSSSGKKNDTTSARELSPSSLKMNKSYLLEPWFLPPSRGLQNSPAVSLPDPEFKRDR
                     KGPHPTARSPGTVMESNPQQREGSSPKHQDHTTDPKLLTCLGQNLHSPDLARPRCPLP
                     PEASPSREKPGLRESSERGPPTARSERSAARADTCREPSMELCFPETAKTSDNSKNLL
                     SVGRTHPDFYTQTQAMEKAWAPGGKTNHKDGPGEARPPPRDNSSLHSAGIPCEKELGK
                     VRRGVEPKPEALLARRSLQPPGIESEKSEKLSSFPSLQKDGAKEPERKEQPLQRHPSS
                     IPPPPLTAKDLSSPAARQHCSSPSHASGREPGAKPSTAEPSSSPQDPPKPVAAHSESS
                     SHKPRPGPDPGPPKTKHPDRSLSSQKPSVGATKGKEPATQSLGGSSREGKGHSKSGPD
                     VFPATPGSQNKASDGIGQGEGGPSVPLHTDRAPLDAKPQPTSGGRPLEVLEKPVHLPR
                     PGHPGPSEPADQKLSAVGEKQTLSPKHPKPSTVKDCPTLCKQTDNRQTDKSPSQPAAN
                     TDRRAEGKKCTEALYAPAEGDKLEAGLSFVHSENRLKGAERPAAGVGKGFPEARGKGP
                     GPQKPPTEADKPNGMKRSPSATGQSSFRSTALPEKSLSCSSSFPETRAGVREASAASS
                     DTSSAKAAGGMLELPAPSNRDHRKAQPAGEGRTHMTKSDSLPSFRVSTLPLESHHPDP
                     NTMGGASHRDRALSVTATVGETKGKDPAPAQPPPARKQNVGRDVTKPSPAPNTDRPIS
                     LSNEKDFVVRQRRGKESLRSSPHKKAL"
     exon            627..780
                     /gene="MAST4"
                     /inference="alignment:Splign:2.1.0"
     exon            781..905
                     /gene="MAST4"
                     /inference="alignment:Splign:2.1.0"
     exon            906..937
                     /gene="MAST4"
                     /inference="alignment:Splign:2.1.0"
     exon            938..1026
                     /gene="MAST4"
                     /inference="alignment:Splign:2.1.0"
     exon            1027..1096
                     /gene="MAST4"
                     /inference="alignment:Splign:2.1.0"
     exon            1097..1175
                     /gene="MAST4"
                     /inference="alignment:Splign:2.1.0"
     exon            1176..1333
                     /gene="MAST4"
                     /inference="alignment:Splign:2.1.0"
     exon            1334..1409
                     /gene="MAST4"
                     /inference="alignment:Splign:2.1.0"
     exon            1410..1619
                     /gene="MAST4"
                     /inference="alignment:Splign:2.1.0"
     exon            1620..1721
                     /gene="MAST4"
                     /inference="alignment:Splign:2.1.0"
     exon            1722..1854
                     /gene="MAST4"
                     /inference="alignment:Splign:2.1.0"
     exon            1855..1922
                     /gene="MAST4"
                     /inference="alignment:Splign:2.1.0"
     exon            1923..2008
                     /gene="MAST4"
                     /inference="alignment:Splign:2.1.0"
     exon            2009..2217
                     /gene="MAST4"
                     /inference="alignment:Splign:2.1.0"
     exon            2218..2356
                     /gene="MAST4"
                     /inference="alignment:Splign:2.1.0"
     exon            2357..2489
                     /gene="MAST4"
                     /inference="alignment:Splign:2.1.0"
     exon            2490..2655
                     /gene="MAST4"
                     /inference="alignment:Splign:2.1.0"
     exon            2656..2757
                     /gene="MAST4"
                     /inference="alignment:Splign:2.1.0"
     exon            2758..2880
                     /gene="MAST4"
                     /inference="alignment:Splign:2.1.0"
     exon            2881..2993
                     /gene="MAST4"
                     /inference="alignment:Splign:2.1.0"
     exon            2994..3121
                     /gene="MAST4"
                     /inference="alignment:Splign:2.1.0"
     exon            3122..3357
                     /gene="MAST4"
                     /inference="alignment:Splign:2.1.0"
     exon            3358..3558
                     /gene="MAST4"
                     /inference="alignment:Splign:2.1.0"
     exon            3559..3788
                     /gene="MAST4"
                     /inference="alignment:Splign:2.1.0"
     exon            3789..3911
                     /gene="MAST4"
                     /inference="alignment:Splign:2.1.0"
     exon            3912..4048
                     /gene="MAST4"
                     /inference="alignment:Splign:2.1.0"
     exon            4049..4230
                     /gene="MAST4"
                     /inference="alignment:Splign:2.1.0"
     exon            4231..10677
                     /gene="MAST4"
                     /inference="alignment:Splign:2.1.0"
     regulatory      10657..10662
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MAST4"
                     /note="hexamer: AATAAA"
     polyA_site      10677
                     /gene="MAST4"
                     /note="major polyA site"
ORIGIN      
        1 acagtggagc gcagatcgcg gacccgagcg ggcatgtccc cgcgcgcggg agcctccgtt
       61 tgcggccggg cccgggcggc tgtgaactta gcagcgggct cctgcggccc cgtcactgcc
      121 atgtagtcgc tggcggggct ccctgcagcc cgggagcggc agtgccagtg agcctgagcc
      181 caggagcccg cgtcttcccc gggaggcgct gagtgcgcgc cgcgcccccg ccgctcggga
      241 ggcactttgg gccagacagg gaaatggggg agaaagtttc ggaggcgcca gagccggtgc
      301 cccgcggctg cagtggccac ggcagccgga ctccagcctc tgcgctggtc gccgcgtcct
      361 ctccgggtgc ttcctcggcc gagtcctcct cgggctcaga aactctgtcg gaggaagggg
      421 agcccggcgg cttctccaga gagcatcagc cgccgccgcc gccgccgttg ggaggcaccc
      481 tgggcgcccg ggcgcccgcc gcgtgggctc cggcaagcgt gctgctggag cgcggagtcc
      541 ttgcgctgcc gccgccgctt cccggaggag ctgtgccgcc cgcgccccgg ggcagcagcg
      601 cgtcccagga ggagcaggac gaggagcttg accacatatt atcccctcca cccatgccgt
      661 ttcggaaatg cagcaaccca gatgtggctt ctggccctgg aaaatcactg aagtataaaa
      721 gacagctgag tgaggatgga agacagctaa ggcgagggag cctgggagga gccctgactg
      781 ggaggtacct tcttccaaac ccggtggcgg gacaggcctg gccggcctct gcagagacgt
      841 ccaacctcgt gcgcatgcgc agccaggccc tgggccagtc ggcgccctcg ctcaccgcca
      901 gcctgaagga gctgagtctc cccagaagag gaagtttttg ccgaacaagc aaccggaaaa
      961 gcttaatagg caatgggcag tcaccagcat tgcctcgacc acactcacct ctctctgctc
     1021 atgcaggaaa tagccctcaa gatagtccaa gaaatttctc ccccagtgcc tcagcccatt
     1081 tttcatttgc acggaggact gatggacgcc gctggtcgtt ggcttctctc ccttcctctg
     1141 gctatgggac aaacacaccc agctctacgg tctcttcatc ctgttcctcc caggagaagt
     1201 tgcatcagtt accataccaa ccaacaccag acgagttaca cttcttatca aaacatttct
     1261 gtaccaccga aagcatcgcc actgagaaca gatgcaggaa cacgccgatg cgcccccgtt
     1321 cccgaagtct gagccctgga cgttctcccg cctgctgtga ccatgaaata attatgatga
     1381 accatgtcta caaagaaagg ttcccaaagg ctacagctca gatggaagaa cgtctaaagg
     1441 aaattatcac cagctactct cctgacaacg ttctaccctt agcagatgga gtgcttagtt
     1501 tcactcacca ccagattatt gaactggctc gagattgctt ggataaatcc caccagggcc
     1561 tcatcacctc acgatacttc cttgaattac agcacaaatt agataagttg ctacaggagg
     1621 ctcatgatcg ttcagaaagt ggagaattgg catttattaa acaactagtt cgaaagatcc
     1681 taattgttat tgcccgccct gctcggttat tagagtgcct ggaatttgat ccggaagaat
     1741 tttactacct attggaagca gcagaaggcc atgccaaaga aggacagggt attaaaaccg
     1801 acattcccag gtacatcatt agccaactgg gactcaataa ggatcccttg gaagaaatgg
     1861 ctcatttggg aaactacgat agtgggacag cagaaacacc agaaacagat gaatcagtga
     1921 gtagctctaa tgcctccctg aaacttcgaa ggaaacctcg ggaaagtgat tttgaaacga
     1981 ttaaattgat tagcaatgga gcctatgggg cagtctactt tgttcggcat aaagaatccc
     2041 ggcagaggtt tgccatgaag aagattaata aacagaacct catccttcga aaccagatcc
     2101 agcaggcctt tgtggagcgg gatatcctga cttttgcaga aaaccccttt gttgtcagca
     2161 tgtattgctc ctttgaaaca aggcgccact tgtgcatggt catggaatat gtggaagggg
     2221 gagactgtgc tactttaatg aaaaacatgg gtcctctccc tgttgatatg gccagaatgt
     2281 actttgctga gacggtcttg gccttggaat atttacataa ttatggaatt gtacacaggg
     2341 atttgaaacc agacaacttg ttggttacct ccatggggca cataaagctg acagattttg
     2401 gattatctaa ggtgggacta atgagcatga ctaccaacct ttacgagggt catattgaga
     2461 aggatgctag agagttcctg gataaacagg tctgtggcac acctgaatac attgcaccag
     2521 aagtgattct gaggcagggt tatggaaagc cggtggactg gtgggccatg gggattatcc
     2581 tctatgaatt tctggttgga tgcgtgccat tctttgggga tactccagag gagctatttg
     2641 gacaagtcat cagtgatgag atcaactggc ctgagaagga tgaggcaccc ccacctgatg
     2701 cccaggatct gattacctta ctcctcaggc agaatcccct ggagaggctg ggaacaggtg
     2761 gtgcatatga agtcaaacag catcgattct tccgttcttt agactggaac agtttgctga
     2821 gacagaaggc agaatttatt ccccaactgg aatctgagga tgacacaagt tattttgata
     2881 ctcggtctga gaagtatcat catatggaaa cggaggaaga agatgacaca aatgatgaag
     2941 actttaatgt ggaaataagg cagttttctt catgttcaca caggttttca aaagttttca
     3001 gcagtataga tcgaatcact cagaattcag cagaagagaa ggaagactct gtggacaaaa
     3061 ccaaaagcac caccttgcca tccacagaaa cactgagctg gagttcagaa tattctgaaa
     3121 tgcaacagct atcaacatcc aactcttcag atactgaaag caacagacat aaactcagtt
     3181 ctggcctact tcccaaactg gctatttcaa cagagggaga gcaagatgaa gctgcctcct
     3241 gccctggaga cccccatgag gagccaggaa agccagccct tcctcctgaa gagtgtgccc
     3301 aggaggagcc tgaggtcacc accccagcca gcaccatcag cagctccacc ctgtcagttg
     3361 gcagtttttc agagcacttg gatcagataa atggacgaag cgagtgtgtg gacagtacag
     3421 ataattcctc aaagccatcc agtgaacccg cttctcacat ggctcggcag cgattagaaa
     3481 gcacagaaaa aaagaaaatc tcggggaaag tcacaaagtc cctctctgcc agtgctcttt
     3541 ccctcatgat cccaggagat atgtttgctg tttcccctct gggaagtcca atgtctcccc
     3601 attccctgtc ctcggaccct tcttcttcac gagattcctc tcccagccga gattcctcag
     3661 cagcttctgc cagtccacat cagccgattg tgatccacag ttcggggaag aactacggct
     3721 ttaccatccg agccatccgg gtgtatgtgg gagacagtga catctataca gtgcaccata
     3781 tcgtctggaa tgtagaagaa ggaagtccgg catgccaggc aggactgaag gctggagatc
     3841 ttatcactca catcaatgga gaaccagtgc atggacttgt ccacacagaa gttatagaac
     3901 tcctactgaa gagtgggaat aaggtgtcaa tcactactac cccatttgaa aacacatcaa
     3961 tcaaaactgg accagccagg agaaacagct ataagagccg gatggtgagg cggagcaaga
     4021 aatccaagaa gaaagaaagt ctcgaaagga ggagatctct tttcaaaaag ctagccaagc
     4081 agccttctcc tttactccac accagccgaa gtttctcctg cttgaacaga tccctgtcat
     4141 cgggtgagag cctcccaggt tcccccactc atagcttgtc tccccggtct ccaacaccaa
     4201 gctaccgctc cacccctgac ttcccatctg gtactaattc ctcccagagc agctccccta
     4261 gttctagtgc ccccaattcc ccagcagggt ccgggcacat ccggcccagc actctccacg
     4321 gtcttgcacc caaactcggc gggcagcggt accggtccgg aaggcgaaag tccgccggca
     4381 acatcccact gtccccgctg gcccggacgc cctctccaac cccgcaaccc acctccccgc
     4441 agcggtcacc atcccctctt ctgggacact cactgggcaa ttccaagatc gcgcaagcct
     4501 ttcccagcaa gatgcactcc ccgcccacca tcgtcagaca catcgtgagg cccaagagtg
     4561 cggagccccc caggtccccg ctgctcaagc gcgtgcagtc cgaggagaag ctgtcgccct
     4621 cttacggcag tgacaagaag cacctgtgct cccgcaagca cagcctggag gtgacccaag
     4681 aggaggtgca gcgggagcag tcccagcggg aggcgccgct gcagagcctg gatgagaacg
     4741 tgtgcgacgt gccgccgctc agccgcgccc ggccagtgga gcaaggctgc ctgaaacgcc
     4801 cagtctcccg gaaggtgggc cgccaggagt ctgtggacga cctggaccgc gacaagctga
     4861 aggccaaggt ggtggtgaag aaagcagacg gcttcccaga gaaacaggaa tcccaccaga
     4921 aatcccatgg acccgggagt gatttggaaa actttgctct gtttaagctg gaagagagag
     4981 agaagaaagt ctatccgaag gctgtggaaa ggtcaagtac ttttgaaaac aaagcgtcta
     5041 tgcaggaggc gccaccgctg ggcagcctgc tgaaggatgc tcttcacaag caggccagcg
     5101 tgcgcgccag cgagggtgcg atgtcggatg gccgggtgcc tgcggagcac cgccagggtg
     5161 gcggggactt cagacgggcc cccgctcctg gcaccctcca ggatggtctc tgccactccc
     5221 tcgacagggg catctctggg aagggggaag gcacggagaa gtcctcccag gccaaggagc
     5281 ttctccgatg tgaaaagtta gacagcaagc tggccaacat cgattacctc cgaaagaaaa
     5341 tgtcacttga ggacaaagag gacaacctct gccctgtgct gaagcccaag atgacagctg
     5401 gctcccacga atgcctgcca gggaacccag tccgacccac gggtgggcag caggagcccc
     5461 cgccggcttc tgagagccga gcttttgtca gcagcaccca tgcagctcag atgagtgccg
     5521 tctcttttgt tcccctcaag gccttaacag gccgggtgga cagtggaacg gagaagcctg
     5581 gcttggttgc tcctgagtcc cctgttagga agagcccctc cgagtataag ctggaaggta
     5641 ggtctgtctc atgcctgaag ccgatcgagg gcactctgga cattgctctc ctgtccggac
     5701 ctcaggcctc caagacagaa ctgccttccc cagagtctgc acagagcccc agcccaagtg
     5761 gtgacgtgag ggcctctgtg ccaccagttc tccccagcag cagtgggaaa aagaacgata
     5821 ccaccagtgc aagagagctt tctccttcca gcttaaagat gaataaatcc tacctgctgg
     5881 agccttggtt cctgcccccc agccgaggtc tccagaattc accagcagtt tccctgcctg
     5941 acccagagtt caagagggac aggaaaggtc cccatcctac tgccaggagc cctggaacag
     6001 tcatggaaag caatccccaa cagagagagg gcagctcccc taaacaccaa gaccacacca
     6061 ctgaccccaa gcttctgacc tgcctggggc agaacctcca cagccctgac ctggccaggc
     6121 cacgctgccc gctcccacct gaagcttccc cctcaaggga gaagccaggc ctgagggaat
     6181 cgtctgaaag aggccctccc acagccagaa gcgagcgctc tgctgcgagg gctgacacat
     6241 gcagagagcc ctccatggaa ctgtgctttc cagaaactgc gaaaaccagt gacaactcca
     6301 aaaatctcct ctctgtggga aggacccacc cagatttcta tacacagacc caggccatgg
     6361 agaaagcatg ggcgccgggt gggaaaacga accacaaaga tggcccaggt gaggcgaggc
     6421 ccccgcccag agacaactcc tctctgcact cagctggaat tccctgtgag aaggagctgg
     6481 gcaaggtgag gcgtggcgtg gaacccaagc ccgaagcgct tcttgccagg cggtctctgc
     6541 agccacctgg aattgagagt gagaagagtg aaaagctctc cagtttccca tctttgcaga
     6601 aagatggtgc caaggaacct gaaaggaagg agcagcctct acaaaggcat cccagcagca
     6661 tccctccgcc ccctctgacg gccaaagacc tgtccagccc ggctgccagg cagcattgca
     6721 gttccccaag ccacgcttct ggcagagagc cgggggccaa gcccagcact gcagagccca
     6781 gctcgagccc ccaggaccct cccaagcctg ttgctgcgca cagtgaaagc agcagccaca
     6841 agccccggcc tggccctgac ccgggccctc caaagactaa gcaccccgac cggtccctct
     6901 cctctcagaa accaagtgtc ggggccacaa agggcaaaga gcctgccact cagtccctcg
     6961 gtggctctag cagagagggg aagggccaca gtaagagtgg gccggatgtg tttcctgcta
     7021 ccccaggctc ccagaacaaa gccagcgatg ggattggcca gggagaaggt gggccctctg
     7081 tcccactgca cactgacagg gctcctctag acgccaagcc acaacccacc agtggtgggc
     7141 ggcccctgga ggtgctggag aagcctgtgc atttgccaag gccgggacac ccagggccta
     7201 gtgagccagc ggaccagaaa ctgtccgctg ttggtgaaaa gcaaaccctg tctccaaagc
     7261 accccaaacc atccactgtg aaagattgcc ccaccctgtg caaacagaca gacaacagac
     7321 agacagacaa aagcccgagt cagccggccg ccaacaccga cagaagggcg gaagggaaga
     7381 aatgcactga agcactttat gctccagcag agggcgacaa gctcgaggcc ggcctttcct
     7441 ttgtgcatag cgagaaccgg ttgaaaggcg cggagcggcc agccgcgggg gtggggaagg
     7501 gcttccctga ggccagaggg aaagggcccg gtccccagaa gccaccgacg gaggcagaca
     7561 agcccaatgg catgaaacgg tccccctcag ccactgggca gagttctttc cgatccacgg
     7621 ccctcccgga aaagtctctg agctgctcct ccagcttccc tgaaaccagg gccggagtta
     7681 gagaggcctc tgcagccagc agcgacacct cttctgccaa ggccgccggg ggcatgctgg
     7741 agcttccagc ccccagcaac agggaccata ggaaggctca gcctgccggg gagggccgaa
     7801 cccacatgac aaagagtgac tccctgccct ccttccgggt ctccaccctg cctctggagt
     7861 cacaccaccc cgacccaaac accatgggcg gggccagcca ccgggacagg gctctctcgg
     7921 tgactgccac cgtaggggaa accaaaggga aggaccctgc cccagcccag cctcccccag
     7981 ctaggaaaca gaacgtgggc agagacgtga ccaagccatc cccagcccca aacactgacc
     8041 gccccatctc tctttctaat gagaaggact ttgtggtacg gcagaggcgg gggaaagaga
     8101 gtttgcgtag cagccctcac aaaaaggcct tgtaacgggg agggcccagg ggcaggactg
     8161 tggagacccg tcctgaacgg gcgactgtgt cttgactacc tttcaaaacc agcactgtgt
     8221 gggaatgtcc gccaggcaga gctcggagcc tcattgagac aggggagaga gaaagacaaa
     8281 gaggggacct tcttccagat gccttcccag ttgtaaccgg taaaactgtt accagatagt
     8341 gtttgtacaa aaaaaaaaaa aaaaaaaaaa aaaaaaatta cctttacagt tgagggttgt
     8401 tgaggaaaat gattttcttt gtaaatatat agcatcgtgt ttggtttggg atgtagagtc
     8461 tatacctggc tgctgattgc gtcgtttact acagcttttt ttaaataaga tttttgcttt
     8521 accatttatc ataatgtagt aatgaagcaa aatggtcaaa actgggtgtg tgtgaacaga
     8581 gatacaccca tattcgtgta tatacacatc cacctacatg ttttggtctg tggtttaaga
     8641 gactatttca aggttccatt tttataagct aaaacattct aagttaagat ggaagaaagc
     8701 cctaaacaca gtagattctg agtttttatg tgtattttaa ccagagtttc tgatagtact
     8761 gtatctggct acctatattt ccagatctaa agcaagaact actctaagta ctgcatttgg
     8821 aatcctcctc cattaggaat ggccaggaca agtggagcta gccattttca ttacacggcc
     8881 atcccaatgg acactggatc cctggtccaa ctgtctaaaa ggccagttgt tcctttctgt
     8941 ggatgtggat gtctggcctt cacctgaggt agagcgggga ctataaacac acatcaactt
     9001 tctttctgtt tgctttcttt tccttctttt caaactctta aactcaggcc tttatgctgt
     9061 gagtgactag tccaagaagc acacattttg tagtagtcct gcaccagccc tgctcttgaa
     9121 taaaaaggaa aattactggc tgcacccaaa tcttctagta cttgagtaat aagcatcagg
     9181 cattggaaga ggttttaaat ttgctttttt gaggaaaaga actgggggtg gattttggca
     9241 tcgtagcata tcgattaaag aataatcagg acatcagata cattttaata catagctggg
     9301 gccttatgtt gtagatgaag cttgctgttt ttagcaagtt cctgggtttc acattcattt
     9361 ctgctgcatc tagtagctcc acacatttca taacctgatc tctttatttg tatgcaaaaa
     9421 atactgtctt aatacagagc agcatttttg taacaaagag actcgctgga gctatttggt
     9481 gcttgaatgt gaccatcctt tttacttttg ctaagcctta tttaaatttt gtataccgtg
     9541 gaatatgtag aatttgtcaa atcattttag tgctgaaggt ttttgatttc ttgtttttgt
     9601 ttctgttgtt gcagtttctt tggtggcttg ttttgtattg taagatggca cttgctgata
     9661 taaatactaa ggcactaaga gaaaatacag ataagtattt ataagttgct tgggaaccat
     9721 agcagaattt tttgtttggt tttgtaagag aaaaaaaatt accaataaat aactgatcta
     9781 gcacccaaac tctcttgggg gatgtcttag tatcttcacg ctataaaatt gttcatctaa
     9841 gcacagcatc atcttatggc cttatggatt gtaaatcttc tagtgaggct ataaactcca
     9901 ctctgaaaac aaaggcataa cttaaccgac tgctcagttg tccttcgttg taaaatgaat
     9961 tggctttggg aaaaaggaaa aaaacaaaca aaaaaagaaa aagaaaaaag cctcctcctt
    10021 gcccctaatt tttttttccc atatggtttt agccattccc catcactata ttgtgaagct
    10081 gtaaaaatat atatatatac tttacaagag tttactaatg gatttcaaaa ctggtctcaa
    10141 cgtttataat acatatctgt gtttggcagt tgtcataacc caatcctaca ttcagccatc
    10201 gggacaagtc tgtcggggag gtagttcatg gtttacaagc ctttgctttt taactgtcca
    10261 atttccttta aagcacaact agctatttgt ttacaaatga tatttttatg tatattttgt
    10321 atagtgtatc atcatttttg ccaaatatgt ttttcattat aaatgagtaa agagtactta
    10381 aggttgcatt catgtattac atgtttgttg ttgtaaacct ctccaatcag ctggtagaaa
    10441 ttctcctcat tgtgttctat ttggtcagtc tctgtgtgat tgtaggatgt gctccttggt
    10501 agtactccca gctgtagatt taccagctta aaagtttgtt aggatctgtg caataaagtg
    10561 tttgcagtct tattttctct aagaaattcc ttatggacgt cataattgtt gtatattgaa
    10621 caaaatattt atacttatgc agttgcataa cattgaaata aaaatttagc atgaaaa
//
