LOCUS       NM_017490               4494 bp    mRNA    linear   PRI 04-MAR-2025
DEFINITION  Homo sapiens microtubule affinity regulating kinase 2 (MARK2),
            transcript variant 1, mRNA.
ACCESSION   NM_017490
VERSION     NM_017490.4
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4494)
  AUTHORS   Han,Y., Li,M., Zhao,B., Wang,H., Liu,Y., Liu,Z., Xu,J. and Yang,R.
  TITLE     MARK2 phosphorylates KIF13A at a 14-3-3 binding site to polarize
            vesicular transport of transferrin receptor within dendrites
  JOURNAL   Proc Natl Acad Sci U S A 121 (20), e2316266121 (2024)
   PUBMED   38709923
  REMARK    GeneRIF: MARK2 phosphorylates KIF13A at a 14-3-3 binding site to
            polarize vesicular transport of transferrin receptor within
            dendrites.
REFERENCE   2  (bases 1 to 4494)
  AUTHORS   Segal,D., Maier,S., Mastromarco,G.J., Qian,W.W., Nabeel-Shah,S.,
            Lee,H., Moore,G., Lacoste,J., Larsen,B., Lin,Z.Y.,
            Selvabaskaran,A., Liu,K., Smibert,C., Zhang,Z., Greenblatt,J.,
            Peng,J., Lee,H.O., Gingras,A.C. and Taipale,M.
  TITLE     A central chaperone-like role for 14-3-3 proteins in human cells
  JOURNAL   Mol Cell 83 (6), 974-993 (2023)
   PUBMED   36931259
REFERENCE   3  (bases 1 to 4494)
  AUTHORS   Natarajan,S.R., Ponnusamy,L. and Manoharan,R.
  TITLE     MARK2/4 promotes Warburg effect and cell growth in non-small cell
            lung carcinoma through the AMPKalpha1/mTOR/HIF-1alpha signaling
            pathway
  JOURNAL   Biochim Biophys Acta Mol Cell Res 1869 (7), 119242 (2022)
   PUBMED   35192892
  REMARK    GeneRIF: MARK2/4 promotes Warburg effect and cell growth in
            non-small cell lung carcinoma through the
            AMPKalpha1/mTOR/HIF-1alpha signaling pathway.
REFERENCE   4  (bases 1 to 4494)
  AUTHORS   Tabassum,Z., Tseng,J.H., Isemann,C., Tian,X., Chen,Y., Herring,L.E.
            and Cohen,T.J.
  TITLE     Identification of a reciprocal negative feedback loop between
            tau-modifying proteins MARK2 kinase and CBP acetyltransferase
  JOURNAL   J Biol Chem 298 (6), 101977 (2022)
   PUBMED   35469920
  REMARK    GeneRIF: Identification of a reciprocal negative feedback loop
            between tau-modifying proteins MARK2 kinase and CBP
            acetyltransferase.
REFERENCE   5  (bases 1 to 4494)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   6  (bases 1 to 4494)
  AUTHORS   Espinosa,L. and Navarro,E.
  TITLE     Human serine/threonine protein kinase EMK1: genomic structure and
            cDNA cloning of isoforms produced by alternative splicing
  JOURNAL   Cytogenet Cell Genet 81 (3-4), 278-282 (1998)
   PUBMED   9730619
REFERENCE   7  (bases 1 to 4494)
  AUTHORS   Drewes,G., Ebneth,A., Preuss,U., Mandelkow,E.M. and Mandelkow,E.
  TITLE     MARK, a novel family of protein kinases that phosphorylate
            microtubule-associated proteins and trigger microtubule disruption
  JOURNAL   Cell 89 (2), 297-308 (1997)
   PUBMED   9108484
REFERENCE   8  (bases 1 to 4494)
  AUTHORS   Courseaux,A., Grosgeorge,J., Gaudray,P., Pannett,A.A., Forbes,S.A.,
            Williamson,C., Bassett,D., Thakker,R.V., Teh,B.T., Farnebo,F.,
            Shepherd,J., Skogseid,B., Larsson,C., Giraud,S., Zhang,C.X.,
            Salandre,J. and Calender,A.
  TITLE     Definition of the minimal MEN1 candidate area based on a 5-Mb
            integrated map of proximal 11q13. The European Consortium on Men1,
            (GENEM 1; Groupe d'Etude des Neoplasies Endocriniennes Multiples de
            type 1)
  JOURNAL   Genomics 37 (3), 354-365 (1996)
   PUBMED   8938448
REFERENCE   9  (bases 1 to 4494)
  AUTHORS   Courseaux,A., Fernandes,M., Grosgeorge,J., Inglis,J., Raynaud,S.D.
            and Gaudray,P.
  TITLE     Human EMK1 is located on 11q12-q13, close to COX8 and FTH1
  JOURNAL   Mamm Genome 6 (4), 311-312 (1995)
   PUBMED   7613050
REFERENCE   10 (bases 1 to 4494)
  AUTHORS   Schultz,S.J. and Nigg,E.A.
  TITLE     Identification of 21 novel human protein kinases, including 3
            members of a family related to the cell cycle regulator nimA of
            Aspergillus nidulans
  JOURNAL   Cell Growth Differ 4 (10), 821-830 (1993)
   PUBMED   8274451
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DA855402.1, AK290339.1,
            BT007342.1, AP003780.3 and BM719402.1.
            
            On Jun 2, 2019 this sequence version replaced NM_017490.3.
            
            Summary: This gene encodes a member of the Par-1 family of
            serine/threonine protein kinases. The protein is an important
            regulator of cell polarity in epithelial and neuronal cells, and
            also controls the stability of microtubules through phosphorylation
            and inactivation of several microtubule-associating proteins. The
            protein localizes to cell membranes. Multiple transcript variants
            encoding different isoforms have been found for this gene.
            [provided by RefSeq, Jul 2009].
            
            Transcript Variant: This variant (1) represents use of an alternate
            promoter and 5' UTR, uses a downstream start codon, lacks an
            in-frame exon, and uses an alternate in-frame splice site, compared
            to variant 4. The resulting isoform (a) has a shorter N-terminus
            and lacks an internal segment and 1 internal residue, compared to
            isoform d.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK290339.1 [ECO:0000332]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-44                DA855402.1         23-66
            45-682              AK290339.1         35-672
            683-960             BT007342.1         210-487
            961-2147            AK290339.1         951-2137
            2148-2148           BT007342.1         1678-1678
            2149-2872           AK290339.1         2139-2862
            2873-4142           AP003780.3         70395-71664
            4143-4494           BM719402.1         24-375
FEATURES             Location/Qualifiers
     source          1..4494
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="11"
                     /map="11q13.1"
     gene            1..4494
                     /gene="MARK2"
                     /gene_synonym="EMK-1; EMK1; PAR-1; Par-1b; Par1b"
                     /note="microtubule affinity regulating kinase 2"
                     /db_xref="GeneID:2011"
                     /db_xref="HGNC:HGNC:3332"
                     /db_xref="MIM:600526"
     exon            1..428
                     /gene="MARK2"
                     /gene_synonym="EMK-1; EMK1; PAR-1; Par-1b; Par1b"
                     /inference="alignment:Splign:2.1.0"
     exon            429..608
                     /gene="MARK2"
                     /gene_synonym="EMK-1; EMK1; PAR-1; Par-1b; Par1b"
                     /inference="alignment:Splign:2.1.0"
     CDS             474..2711
                     /gene="MARK2"
                     /gene_synonym="EMK-1; EMK1; PAR-1; Par-1b; Par1b"
                     /EC_number="2.7.11.1"
                     /EC_number="2.7.11.26"
                     /note="isoform a is encoded by transcript variant 1;
                     Ser/Thr protein kinase PAR-1B; serine/threonine protein
                     kinase EMK; MAP/microtubule affinity-regulating kinase 2;
                     serine/threonine-protein kinase MARK2; ELKL motif kinase
                     1; PAR1 homolog b; testicular tissue protein Li 117"
                     /codon_start=1
                     /product="serine/threonine-protein kinase MARK2 isoform a"
                     /protein_id="NP_059672.2"
                     /db_xref="CCDS:CCDS41665.1"
                     /db_xref="GeneID:2011"
                     /db_xref="HGNC:HGNC:3332"
                     /db_xref="MIM:600526"
                     /translation="MIRGRNSATSADEQPHIGNYRLLKTIGKGNFAKVKLARHILTGK
                     EVAVKIIDKTQLNSSSLQKLFREVRIMKVLNHPNIVKLFEVIETEKTLYLVMEYASGG
                     EVFDYLVAHGRMKEKEARAKFRQIVSAVQYCHQKFIVHRDLKAENLLLDADMNIKIAD
                     FGFSNEFTFGNKLDTFCGSPPYAAPELFQGKKYDGPEVDVWSLGVILYTLVSGSLPFD
                     GQNLKELRERVLRGKYRIPFYMSTDCENLLKKFLILNPSKRGTLEQIMKDRWMNVGHE
                     DDELKPYVEPLPDYKDPRRTELMVSMGYTREEIQDSLVGQRYNEVMATYLLLGYKSSE
                     LEGDTITLKPRPSADLTNSSAPSPSHKVQRSVSANPKQRRFSDQAGPAIPTSNSYSKK
                     TQSNNAENKRPEEDRESGRKASSTAKVPASPLPGLERKKTTPTPSTNSVLSTSTNRSR
                     NSPLLERASLGQASIQNGKDSLTMPGSRASTASASAAVSAARPRQHQKSMSASVHPNK
                     ASGLPPTESNCEVPRPSTAPQRVPVASPSAHNISSSGGAPDRTNFPRGVSSRSTFHAG
                     QLRQVRDQQNLPYGVTPASPSGHSQGRRGASGSIFSKFTSKFVRRNLNEPESKDRVET
                     LRPHVVGSGGNDKEKEEFREAKPRSLRFTWSMKTTSSMEPNEMMREIRKVLDANSCQS
                     ELHEKYMLLCMHGTPGHEDFVQWEMEVCKLPRLSLNGVRFKRISGTSMAFKNIASKIA
                     NELKL"
     exon            609..662
                     /gene="MARK2"
                     /gene_synonym="EMK-1; EMK1; PAR-1; Par-1b; Par1b"
                     /inference="alignment:Splign:2.1.0"
     exon            663..711
                     /gene="MARK2"
                     /gene_synonym="EMK-1; EMK1; PAR-1; Par-1b; Par1b"
                     /inference="alignment:Splign:2.1.0"
     exon            712..777
                     /gene="MARK2"
                     /gene_synonym="EMK-1; EMK1; PAR-1; Par-1b; Par1b"
                     /inference="alignment:Splign:2.1.0"
     exon            778..848
                     /gene="MARK2"
                     /gene_synonym="EMK-1; EMK1; PAR-1; Par-1b; Par1b"
                     /inference="alignment:Splign:2.1.0"
     exon            849..905
                     /gene="MARK2"
                     /gene_synonym="EMK-1; EMK1; PAR-1; Par-1b; Par1b"
                     /inference="alignment:Splign:2.1.0"
     exon            906..1142
                     /gene="MARK2"
                     /gene_synonym="EMK-1; EMK1; PAR-1; Par-1b; Par1b"
                     /inference="alignment:Splign:2.1.0"
     exon            1143..1262
                     /gene="MARK2"
                     /gene_synonym="EMK-1; EMK1; PAR-1; Par-1b; Par1b"
                     /inference="alignment:Splign:2.1.0"
     exon            1263..1362
                     /gene="MARK2"
                     /gene_synonym="EMK-1; EMK1; PAR-1; Par-1b; Par1b"
                     /inference="alignment:Splign:2.1.0"
     exon            1363..1475
                     /gene="MARK2"
                     /gene_synonym="EMK-1; EMK1; PAR-1; Par-1b; Par1b"
                     /inference="alignment:Splign:2.1.0"
     exon            1476..1608
                     /gene="MARK2"
                     /gene_synonym="EMK-1; EMK1; PAR-1; Par-1b; Par1b"
                     /inference="alignment:Splign:2.1.0"
     exon            1609..1787
                     /gene="MARK2"
                     /gene_synonym="EMK-1; EMK1; PAR-1; Par-1b; Par1b"
                     /inference="alignment:Splign:2.1.0"
     exon            1788..1885
                     /gene="MARK2"
                     /gene_synonym="EMK-1; EMK1; PAR-1; Par-1b; Par1b"
                     /inference="alignment:Splign:2.1.0"
     exon            1886..2047
                     /gene="MARK2"
                     /gene_synonym="EMK-1; EMK1; PAR-1; Par-1b; Par1b"
                     /inference="alignment:Splign:2.1.0"
     exon            2048..2305
                     /gene="MARK2"
                     /gene_synonym="EMK-1; EMK1; PAR-1; Par-1b; Par1b"
                     /inference="alignment:Splign:2.1.0"
     exon            2306..2350
                     /gene="MARK2"
                     /gene_synonym="EMK-1; EMK1; PAR-1; Par-1b; Par1b"
                     /inference="alignment:Splign:2.1.0"
     exon            2351..4494
                     /gene="MARK2"
                     /gene_synonym="EMK-1; EMK1; PAR-1; Par-1b; Par1b"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4470..4475
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MARK2"
                     /gene_synonym="EMK-1; EMK1; PAR-1; Par-1b; Par1b"
                     /note="hexamer: AATAAA"
     polyA_site      4494
                     /gene="MARK2"
                     /gene_synonym="EMK-1; EMK1; PAR-1; Par-1b; Par1b"
                     /note="major polyA site"
ORIGIN      
        1 ctctgtctcc cttcctgccc tattcccctc ctgccccttc cctcccacct tgcttctggt
       61 gtgctgtcct ggaattgcac gcgcttcctg accaccaggc tctggccctt gagaagccag
      121 cggggctttg tccctgttgc tctccttgcc aaacccagtc tctctgctag tggtggtttc
      181 ggttgcgaca ccgtccaggt tcccaggcag gaaccgctcg gcctggctgc ttagctactt
      241 ttcactgagg aggtggtgga aggtgtcgcc tgctctggct gagtaagggt ggctggctga
      301 gccggcagcc cccgccctag gcctggctct tcccggcctc tgtactttgc cctcgctgcc
      361 tgacaggttc tgctgtgggc tctgctgaat ggaagtcgct ggtagtcctt ttccctttct
      421 ccagtcggcc caccttggga caccttgact ccaagcccag cagtaagtcc aacatgattc
      481 ggggccgcaa ctcagccacc tctgctgatg agcagcccca cattggaaac taccggctcc
      541 tcaagaccat tggcaagggt aattttgcca aggtgaagtt ggcccgacac atcctgactg
      601 ggaaagaggt agctgtgaag atcattgaca agactcaact gaactcctcc agcctccaga
      661 aactattccg cgaagtaaga ataatgaagg ttttgaatca tcccaacata gttaaattat
      721 ttgaagtgat tgagactgag aaaacgctct accttgtcat ggagtacgct agtggcggag
      781 aggtatttga ttacctagtg gctcatggca ggatgaaaga aaaagaggct cgagccaaat
      841 tccgccagat agtgtctgct gtgcagtact gtcaccagaa gtttattgtc catagagact
      901 taaaggcaga aaacctgctc ttggatgctg atatgaacat caagattgca gactttggct
      961 tcagcaatga attcaccttt gggaacaagc tggacacctt ctgtggcagt cccccttatg
     1021 ctgccccaga actcttccag ggcaaaaaat atgatggacc cgaggtggat gtgtggagcc
     1081 taggagttat cctctataca ctggtcagcg gatccctgcc ttttgatgga cagaacctca
     1141 aggagctgcg ggaacgggta ctgaggggaa aataccgtat tccattctac atgtccacgg
     1201 actgtgaaaa cctgcttaag aaatttctca ttcttaatcc cagcaagaga ggcactttag
     1261 agcaaatcat gaaagatcga tggatgaatg tgggtcacga agatgatgaa ctaaagcctt
     1321 acgtggagcc actccctgac tacaaggacc cccggcggac agagctgatg gtgtccatgg
     1381 gttatacacg ggaagagatc caggactcgc tggtgggcca gagatacaac gaggtgatgg
     1441 ccacctatct gctcctgggc tacaagagct ccgagctgga aggcgacacc atcaccctga
     1501 aaccccggcc ttcagctgat ctgaccaata gcagcgcccc atccccatcc cacaaggtac
     1561 agcgcagcgt gtcggccaat cccaagcagc ggcgcttcag cgaccaggct ggtcctgcca
     1621 ttcccacctc taattcttac tctaagaaga ctcagagtaa caacgcagaa aataagcggc
     1681 ctgaggagga ccgggagtca gggcggaaag ccagcagcac agccaaggtg cctgccagcc
     1741 ccctgcccgg tctggagagg aagaagacca ccccaacccc ctccacgaac agcgtcctct
     1801 ccaccagcac aaatcgaagc aggaattccc cacttttgga gcgggccagc ctcggccagg
     1861 cctccatcca gaatggcaaa gacagcctaa ccatgccagg gtcccgggcc tccacggctt
     1921 ctgcttctgc cgcagtctct gcggcccggc cccgccagca ccagaaatcc atgtcggcct
     1981 ccgtgcaccc caacaaggcc tctgggctgc cccccacgga gagtaactgt gaggtgccgc
     2041 ggcccagcac agccccccag cgtgtccctg ttgcctcccc atccgcccac aacatcagca
     2101 gcagtggtgg agccccagac cgaactaact tcccccgggg tgtgtccagc cgaagcacct
     2161 tccatgctgg gcagctccga caggtgcggg accagcagaa tttgccctac ggtgtgaccc
     2221 cagcctctcc ctctggccac agccagggcc ggcggggggc ctctgggagc atcttcagca
     2281 agttcacctc caagtttgta cgcaggaacc tgaatgaacc tgaaagcaaa gaccgagtgg
     2341 agacgctcag acctcacgtg gtgggcagtg gcggcaacga caaagaaaag gaagaatttc
     2401 gggaggccaa gccccgctcc ctccgcttca cgtggagtat gaagaccacg agctccatgg
     2461 agcccaacga gatgatgcgg gagatccgca aggtgctgga cgcgaacagc tgccagagcg
     2521 agctgcatga gaagtacatg ctgctgtgca tgcacggcac gccgggccac gaggacttcg
     2581 tgcagtggga gatggaggtg tgcaaactgc cgcggctctc tctcaacggg gttcgattta
     2641 agcggatatc gggcacctcc atggccttca aaaacattgc ctccaaaata gccaacgagc
     2701 tgaagcttta acaggctgcc aggagcgggg gcggcggggg cgggccagct ggacgggctg
     2761 ccggccgctg cgccgcccca cctgggcgag actgcagcga tggattggtg tgtctcccct
     2821 gctggcactt ctcccctccc tggcccttct cagttttctc ttacatgttt gtggggggtg
     2881 ggagattgtt ctccagcacc ccacattcac ccctgcccag agattccccc ttctcctctc
     2941 ccctactgga ggcaaaggaa ggggagggtg gatggggggg cagggctccc cctcggtact
     3001 gcggttgcac agagtatttc gcctaaacca agaaattttt tattaccaaa aagaaaaaag
     3061 aaaaaaaaaa tcccagcggc cacctttcct ccctgcccca ttgggacagt cgagactgga
     3121 tctgtggggt ttcccgggag ggtggctcag ggctggaaca ctctcaggca agagtggtgg
     3181 agctcccgtc aggccctccg ccaggcccac tgtgggcttc tcccctctcc tccctccttc
     3241 ccctccaagc aaaccaccag aggtggcctt cccctgacct caggcccctg ggctggaggc
     3301 ctgggcggtg gggcaggggg cgggggtgct gcgcagccct gcagtgggtg gggctggggg
     3361 ctgctccggg gctgctgagg ctggagggcc ggcacaaggc tccgcctccc tccacactgt
     3421 accctctgcc cctcctcccc agagctgggc atttccttcc acaagctgct gtggggacgt
     3481 gtgttccctc aaagtctgtg ccatcttctc ccacccctcc cgggtagaag gaggggctga
     3541 ccccagggct gggagagggg aggggactgg agggcagact ggcttctcgg tccccagggg
     3601 gccgcttggg ctgttggtct ccagagcagg gccactgggc actctgtgat gggggagcct
     3661 ttgtctgaaa gcacagcccc ctcgcccttc ctctccccat ggcttcccct tcattggcat
     3721 taatctgggc accagctctc tccatagcag tgacttccct caccactctc atctctcagc
     3781 cttgcctttt cttcctgaca ctgtcgcccc ctcctctcag gagacactgc cgagggccac
     3841 ctggcagaag gctgagttag gcagcagggc cgggagcgtc tgccctccac agggtggggg
     3901 acagataggc taagcgactc ccagcttgct accctcagtg gccagtgtgg gcgtgggcgg
     3961 tttggggcgc ttggctggtg gtggccactg catcccttaa tttatttctc tgctgtttct
     4021 gttcttgaga aattgggggt gggagtccta cacagaggct gcccctaccc tcacctgagt
     4081 tgtacatttt tttgtgatgg gttttatttt ttattatttt attttatttt tttttttttt
     4141 gatttatgat gactccaccc ctcttcatca cccccgctcc caggccaggc tcagcgatta
     4201 agccgagccc ttgcgtccta ggaaggggcc ttgccaacct cagccctcct gccccacact
     4261 cctactgcgg ctcagaccaa gggctccccc tccctccctt cccccctcct gccctatgga
     4321 acagcccggg tgctctgagg gggctgggag ggcatggctt ggctcccaaa gggggtaggg
     4381 gcccggggca cccaggcaag gtggcccctc cccgtctagc cccctcctcc ccaaccctgc
     4441 acttagtttc tcctctggat caaacacgta ataaagagaa tgtttggaat ctga
//
