LOCUS       NM_001039469            4547 bp    mRNA    linear   PRI 04-MAR-2025
DEFINITION  Homo sapiens microtubule affinity regulating kinase 2 (MARK2),
            transcript variant 4, mRNA.
ACCESSION   NM_001039469
VERSION     NM_001039469.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4547)
  AUTHORS   Han,Y., Li,M., Zhao,B., Wang,H., Liu,Y., Liu,Z., Xu,J. and Yang,R.
  TITLE     MARK2 phosphorylates KIF13A at a 14-3-3 binding site to polarize
            vesicular transport of transferrin receptor within dendrites
  JOURNAL   Proc Natl Acad Sci U S A 121 (20), e2316266121 (2024)
   PUBMED   38709923
  REMARK    GeneRIF: MARK2 phosphorylates KIF13A at a 14-3-3 binding site to
            polarize vesicular transport of transferrin receptor within
            dendrites.
REFERENCE   2  (bases 1 to 4547)
  AUTHORS   Segal,D., Maier,S., Mastromarco,G.J., Qian,W.W., Nabeel-Shah,S.,
            Lee,H., Moore,G., Lacoste,J., Larsen,B., Lin,Z.Y.,
            Selvabaskaran,A., Liu,K., Smibert,C., Zhang,Z., Greenblatt,J.,
            Peng,J., Lee,H.O., Gingras,A.C. and Taipale,M.
  TITLE     A central chaperone-like role for 14-3-3 proteins in human cells
  JOURNAL   Mol Cell 83 (6), 974-993 (2023)
   PUBMED   36931259
REFERENCE   3  (bases 1 to 4547)
  AUTHORS   Natarajan,S.R., Ponnusamy,L. and Manoharan,R.
  TITLE     MARK2/4 promotes Warburg effect and cell growth in non-small cell
            lung carcinoma through the AMPKalpha1/mTOR/HIF-1alpha signaling
            pathway
  JOURNAL   Biochim Biophys Acta Mol Cell Res 1869 (7), 119242 (2022)
   PUBMED   35192892
  REMARK    GeneRIF: MARK2/4 promotes Warburg effect and cell growth in
            non-small cell lung carcinoma through the
            AMPKalpha1/mTOR/HIF-1alpha signaling pathway.
REFERENCE   4  (bases 1 to 4547)
  AUTHORS   Tabassum,Z., Tseng,J.H., Isemann,C., Tian,X., Chen,Y., Herring,L.E.
            and Cohen,T.J.
  TITLE     Identification of a reciprocal negative feedback loop between
            tau-modifying proteins MARK2 kinase and CBP acetyltransferase
  JOURNAL   J Biol Chem 298 (6), 101977 (2022)
   PUBMED   35469920
  REMARK    GeneRIF: Identification of a reciprocal negative feedback loop
            between tau-modifying proteins MARK2 kinase and CBP
            acetyltransferase.
REFERENCE   5  (bases 1 to 4547)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   6  (bases 1 to 4547)
  AUTHORS   Espinosa,L. and Navarro,E.
  TITLE     Human serine/threonine protein kinase EMK1: genomic structure and
            cDNA cloning of isoforms produced by alternative splicing
  JOURNAL   Cytogenet Cell Genet 81 (3-4), 278-282 (1998)
   PUBMED   9730619
REFERENCE   7  (bases 1 to 4547)
  AUTHORS   Drewes,G., Ebneth,A., Preuss,U., Mandelkow,E.M. and Mandelkow,E.
  TITLE     MARK, a novel family of protein kinases that phosphorylate
            microtubule-associated proteins and trigger microtubule disruption
  JOURNAL   Cell 89 (2), 297-308 (1997)
   PUBMED   9108484
REFERENCE   8  (bases 1 to 4547)
  AUTHORS   Courseaux,A., Grosgeorge,J., Gaudray,P., Pannett,A.A., Forbes,S.A.,
            Williamson,C., Bassett,D., Thakker,R.V., Teh,B.T., Farnebo,F.,
            Shepherd,J., Skogseid,B., Larsson,C., Giraud,S., Zhang,C.X.,
            Salandre,J. and Calender,A.
  TITLE     Definition of the minimal MEN1 candidate area based on a 5-Mb
            integrated map of proximal 11q13. The European Consortium on Men1,
            (GENEM 1; Groupe d'Etude des Neoplasies Endocriniennes Multiples de
            type 1)
  JOURNAL   Genomics 37 (3), 354-365 (1996)
   PUBMED   8938448
REFERENCE   9  (bases 1 to 4547)
  AUTHORS   Courseaux,A., Fernandes,M., Grosgeorge,J., Inglis,J., Raynaud,S.D.
            and Gaudray,P.
  TITLE     Human EMK1 is located on 11q12-q13, close to COX8 and FTH1
  JOURNAL   Mamm Genome 6 (4), 311-312 (1995)
   PUBMED   7613050
REFERENCE   10 (bases 1 to 4547)
  AUTHORS   Schultz,S.J. and Nigg,E.A.
  TITLE     Identification of 21 novel human protein kinases, including 3
            members of a family related to the cell cycle regulator nimA of
            Aspergillus nidulans
  JOURNAL   Cell Growth Differ 4 (10), 821-830 (1993)
   PUBMED   8274451
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AB188493.1, AK225766.1,
            BC008771.2, AP003780.3 and BM719402.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Aug 13, 2020 this sequence version replaced NM_001039469.2.
            
            Summary: This gene encodes a member of the Par-1 family of
            serine/threonine protein kinases. The protein is an important
            regulator of cell polarity in epithelial and neuronal cells, and
            also controls the stability of microtubules through phosphorylation
            and inactivation of several microtubule-associating proteins. The
            protein localizes to cell membranes. Multiple transcript variants
            encoding different isoforms have been found for this gene.
            [provided by RefSeq, Jul 2009].
            
            Transcript Variant: This variant (4) represents the longest
            transcript and encodes the longest isoform (d).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR14038194.1256064.1,
                                           SRR14038192.1146772.1 [ECO:0000332]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000402010.8/ ENSP00000385751.2
            RefSeq Select criteria :: based on conservation, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-191               AB188493.1         186-376
            192-447             AK225766.1         209-464
            448-1881            BC008771.2         22-1455
            1882-1882           AK225766.1         1896-1896
            1883-2950           BC008771.2         1457-2524
            2951-4195           AP003780.3         70420-71664
            4196-4547           BM719402.1         24-375
FEATURES             Location/Qualifiers
     source          1..4547
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="11"
                     /map="11q13.1"
     gene            1..4547
                     /gene="MARK2"
                     /gene_synonym="EMK-1; EMK1; PAR-1; Par-1b; Par1b"
                     /note="microtubule affinity regulating kinase 2"
                     /db_xref="GeneID:2011"
                     /db_xref="HGNC:HGNC:3332"
                     /db_xref="MIM:600526"
     exon            1..451
                     /gene="MARK2"
                     /gene_synonym="EMK-1; EMK1; PAR-1; Par-1b; Par1b"
                     /inference="alignment:Splign:2.1.0"
     CDS             398..2764
                     /gene="MARK2"
                     /gene_synonym="EMK-1; EMK1; PAR-1; Par-1b; Par1b"
                     /EC_number="2.7.11.1"
                     /EC_number="2.7.11.26"
                     /note="isoform d is encoded by transcript variant 4;
                     Ser/Thr protein kinase PAR-1B; serine/threonine protein
                     kinase EMK; MAP/microtubule affinity-regulating kinase 2;
                     serine/threonine-protein kinase MARK2; ELKL motif kinase
                     1; PAR1 homolog b; testicular tissue protein Li 117"
                     /codon_start=1
                     /product="serine/threonine-protein kinase MARK2 isoform d"
                     /protein_id="NP_001034558.2"
                     /db_xref="CCDS:CCDS53649.1"
                     /db_xref="GeneID:2011"
                     /db_xref="HGNC:HGNC:3332"
                     /db_xref="MIM:600526"
                     /translation="MSSARTPLPTLNERDTEQPTLGHLDSKPSSKSNMIRGRNSATSA
                     DEQPHIGNYRLLKTIGKGNFAKVKLARHILTGKEVAVKIIDKTQLNSSSLQKLFREVR
                     IMKVLNHPNIVKLFEVIETEKTLYLVMEYASGGEVFDYLVAHGRMKEKEARAKFRQIV
                     SAVQYCHQKFIVHRDLKAENLLLDADMNIKIADFGFSNEFTFGNKLDTFCGSPPYAAP
                     ELFQGKKYDGPEVDVWSLGVILYTLVSGSLPFDGQNLKELRERVLRGKYRIPFYMSTD
                     CENLLKKFLILNPSKRGTLEQIMKDRWMNVGHEDDELKPYVEPLPDYKDPRRTELMVS
                     MGYTREEIQDSLVGQRYNEVMATYLLLGYKSSELEGDTITLKPRPSADLTNSSAPSPS
                     HKVQRSVSANPKQRRFSDQAAGPAIPTSNSYSKKTQSNNAENKRPEEDRESGRKASST
                     AKVPASPLPGLERKKTTPTPSTNSVLSTSTNRSRNSPLLERASLGQASIQNGKDSLTM
                     PGSRASTASASAAVSAARPRQHQKSMSASVHPNKASGLPPTESNCEVPRPSTAPQRVP
                     VASPSAHNISSSGGAPDRTNFPRGVSSRSTFHAGQLRQVRDQQNLPYGVTPASPSGHS
                     QGRRGASGSIFSKFTSKFVRRNLSFRFARRNLNEPESKDRVETLRPHVVGSGGNDKEK
                     EEFREAKPRSLRFTWSMKTTSSMEPNEMMREIRKVLDANSCQSELHEKYMLLCMHGTP
                     GHEDFVQWEMEVCKLPRLSLNGVRFKRISGTSMAFKNIASKIANELKL"
     exon            452..631
                     /gene="MARK2"
                     /gene_synonym="EMK-1; EMK1; PAR-1; Par-1b; Par1b"
                     /inference="alignment:Splign:2.1.0"
     exon            632..685
                     /gene="MARK2"
                     /gene_synonym="EMK-1; EMK1; PAR-1; Par-1b; Par1b"
                     /inference="alignment:Splign:2.1.0"
     exon            686..734
                     /gene="MARK2"
                     /gene_synonym="EMK-1; EMK1; PAR-1; Par-1b; Par1b"
                     /inference="alignment:Splign:2.1.0"
     exon            735..800
                     /gene="MARK2"
                     /gene_synonym="EMK-1; EMK1; PAR-1; Par-1b; Par1b"
                     /inference="alignment:Splign:2.1.0"
     exon            801..871
                     /gene="MARK2"
                     /gene_synonym="EMK-1; EMK1; PAR-1; Par-1b; Par1b"
                     /inference="alignment:Splign:2.1.0"
     exon            872..928
                     /gene="MARK2"
                     /gene_synonym="EMK-1; EMK1; PAR-1; Par-1b; Par1b"
                     /inference="alignment:Splign:2.1.0"
     exon            929..1165
                     /gene="MARK2"
                     /gene_synonym="EMK-1; EMK1; PAR-1; Par-1b; Par1b"
                     /inference="alignment:Splign:2.1.0"
     exon            1166..1285
                     /gene="MARK2"
                     /gene_synonym="EMK-1; EMK1; PAR-1; Par-1b; Par1b"
                     /inference="alignment:Splign:2.1.0"
     exon            1286..1385
                     /gene="MARK2"
                     /gene_synonym="EMK-1; EMK1; PAR-1; Par-1b; Par1b"
                     /inference="alignment:Splign:2.1.0"
     exon            1386..1498
                     /gene="MARK2"
                     /gene_synonym="EMK-1; EMK1; PAR-1; Par-1b; Par1b"
                     /inference="alignment:Splign:2.1.0"
     exon            1499..1631
                     /gene="MARK2"
                     /gene_synonym="EMK-1; EMK1; PAR-1; Par-1b; Par1b"
                     /inference="alignment:Splign:2.1.0"
     exon            1632..1813
                     /gene="MARK2"
                     /gene_synonym="EMK-1; EMK1; PAR-1; Par-1b; Par1b"
                     /inference="alignment:Splign:2.1.0"
     exon            1814..1911
                     /gene="MARK2"
                     /gene_synonym="EMK-1; EMK1; PAR-1; Par-1b; Par1b"
                     /inference="alignment:Splign:2.1.0"
     exon            1912..2073
                     /gene="MARK2"
                     /gene_synonym="EMK-1; EMK1; PAR-1; Par-1b; Par1b"
                     /inference="alignment:Splign:2.1.0"
     exon            2074..2331
                     /gene="MARK2"
                     /gene_synonym="EMK-1; EMK1; PAR-1; Par-1b; Par1b"
                     /inference="alignment:Splign:2.1.0"
     exon            2332..2358
                     /gene="MARK2"
                     /gene_synonym="EMK-1; EMK1; PAR-1; Par-1b; Par1b"
                     /inference="alignment:Splign:2.1.0"
     exon            2359..2403
                     /gene="MARK2"
                     /gene_synonym="EMK-1; EMK1; PAR-1; Par-1b; Par1b"
                     /inference="alignment:Splign:2.1.0"
     exon            2404..4547
                     /gene="MARK2"
                     /gene_synonym="EMK-1; EMK1; PAR-1; Par-1b; Par1b"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4523..4528
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MARK2"
                     /gene_synonym="EMK-1; EMK1; PAR-1; Par-1b; Par1b"
                     /note="hexamer: AATAAA"
     polyA_site      4547
                     /gene="MARK2"
                     /gene_synonym="EMK-1; EMK1; PAR-1; Par-1b; Par1b"
                     /note="major polyA site"
ORIGIN      
        1 ggcggtggtg gcggccatgt tgggagcagc aggtccggcg gcggctgcct gtgtgccggg
       61 cgcggagcag tgccgctgag ggcaggggag gagcgaggca ggcggccggc tgcggcggca
      121 gagagtaggc ggagcggcgc ggcccggccg aaaggcggca cagcccagcc gggggtcggg
      181 ggggtgcggt ccggagccgc tcggagccgg cgcggcctag cccgagcggc gcatccccgg
      241 gctggcgtga gcggctgccc ggcctccccg cacccccggc cggggcccat gcggcgggtg
      301 ctcctgctgt gagaagcccc gcccggccgg gctccgcgcc ttcccttccc tcccttcctc
      361 caagcttctc ggttccctcc cccgagatac cggcgccatg tccagcgctc ggacccccct
      421 acccacgctg aacgagaggg acacggagca gcccaccttg ggacaccttg actccaagcc
      481 cagcagtaag tccaacatga ttcggggccg caactcagcc acctctgctg atgagcagcc
      541 ccacattgga aactaccggc tcctcaagac cattggcaag ggtaattttg ccaaggtgaa
      601 gttggcccga cacatcctga ctgggaaaga ggtagctgtg aagatcattg acaagactca
      661 actgaactcc tccagcctcc agaaactatt ccgcgaagta agaataatga aggttttgaa
      721 tcatcccaac atagttaaat tatttgaagt gattgagact gagaaaacgc tctaccttgt
      781 catggagtac gctagtggcg gagaggtatt tgattaccta gtggctcatg gcaggatgaa
      841 agaaaaagag gctcgagcca aattccgcca gatagtgtct gctgtgcagt actgtcacca
      901 gaagtttatt gtccatagag acttaaaggc agaaaacctg ctcttggatg ctgatatgaa
      961 catcaagatt gcagactttg gcttcagcaa tgaattcacc tttgggaaca agctggacac
     1021 cttctgtggc agtccccctt atgctgcccc agaactcttc cagggcaaaa aatatgatgg
     1081 acccgaggtg gatgtgtgga gcctaggagt tatcctctat acactggtca gcggatccct
     1141 gccttttgat ggacagaacc tcaaggagct gcgggaacgg gtactgaggg gaaaataccg
     1201 tattccattc tacatgtcca cggactgtga aaacctgctt aagaaatttc tcattcttaa
     1261 tcccagcaag agaggcactt tagagcaaat catgaaagat cgatggatga atgtgggtca
     1321 cgaagatgat gaactaaagc cttacgtgga gccactccct gactacaagg acccccggcg
     1381 gacagagctg atggtgtcca tgggttatac acgggaagag atccaggact cgctggtggg
     1441 ccagagatac aacgaggtga tggccaccta tctgctcctg ggctacaaga gctccgagct
     1501 ggaaggcgac accatcaccc tgaaaccccg gccttcagct gatctgacca atagcagcgc
     1561 cccatcccca tcccacaagg tacagcgcag cgtgtcggcc aatcccaagc agcggcgctt
     1621 cagcgaccag gcagctggtc ctgccattcc cacctctaat tcttactcta agaagactca
     1681 gagtaacaac gcagaaaata agcggcctga ggaggaccgg gagtcagggc ggaaagccag
     1741 cagcacagcc aaggtgcctg ccagccccct gcccggtctg gagaggaaga agaccacccc
     1801 aaccccctcc acgaacagcg tcctctccac cagcacaaat cgaagcagga attccccact
     1861 tttggagcgg gccagcctcg gccaggcctc catccagaat ggcaaagaca gcctaaccat
     1921 gccagggtcc cgggcctcca cggcttctgc ttctgccgca gtctctgcgg cccggccccg
     1981 ccagcaccag aaatccatgt cggcctccgt gcaccccaac aaggcctctg ggctgccccc
     2041 cacggagagt aactgtgagg tgccgcggcc cagcacagcc ccccagcgtg tccctgttgc
     2101 ctccccatcc gcccacaaca tcagcagcag tggtggagcc ccagaccgaa ctaacttccc
     2161 ccggggtgtg tccagccgaa gcaccttcca tgctgggcag ctccgacagg tgcgggacca
     2221 gcagaatttg ccctacggtg tgaccccagc ctctccctct ggccacagcc agggccggcg
     2281 gggggcctct gggagcatct tcagcaagtt cacctccaag tttgtacgca gaaatctgtc
     2341 tttcaggttt gccagaagga acctgaatga acctgaaagc aaagaccgag tggagacgct
     2401 cagacctcac gtggtgggca gtggcggcaa cgacaaagaa aaggaagaat ttcgggaggc
     2461 caagccccgc tccctccgct tcacgtggag tatgaagacc acgagctcca tggagcccaa
     2521 cgagatgatg cgggagatcc gcaaggtgct ggacgcgaac agctgccaga gcgagctgca
     2581 tgagaagtac atgctgctgt gcatgcacgg cacgccgggc cacgaggact tcgtgcagtg
     2641 ggagatggag gtgtgcaaac tgccgcggct ctctctcaac ggggttcgat ttaagcggat
     2701 atcgggcacc tccatggcct tcaaaaacat tgcctccaaa atagccaacg agctgaagct
     2761 ttaacaggct gccaggagcg ggggcggcgg gggcgggcca gctggacggg ctgccggccg
     2821 ctgcgccgcc ccacctgggc gagactgcag cgatggattg gtgtgtctcc cctgctggca
     2881 cttctcccct ccctggccct tctcagtttt ctcttacatg tttgtggggg gtgggagatt
     2941 gttctccagc accccacatt cacccctgcc cagagattcc cccttctcct ctcccctact
     3001 ggaggcaaag gaaggggagg gtggatgggg gggcagggct ccccctcggt actgcggttg
     3061 cacagagtat ttcgcctaaa ccaagaaatt ttttattacc aaaaagaaaa aagaaaaaaa
     3121 aaatcccagc ggccaccttt cctccctgcc ccattgggac agtcgagact ggatctgtgg
     3181 ggtttcccgg gagggtggct cagggctgga acactctcag gcaagagtgg tggagctccc
     3241 gtcaggccct ccgccaggcc cactgtgggc ttctcccctc tcctccctcc ttcccctcca
     3301 agcaaaccac cagaggtggc cttcccctga cctcaggccc ctgggctgga ggcctgggcg
     3361 gtggggcagg gggcgggggt gctgcgcagc cctgcagtgg gtggggctgg gggctgctcc
     3421 ggggctgctg aggctggagg gccggcacaa ggctccgcct ccctccacac tgtaccctct
     3481 gcccctcctc cccagagctg ggcatttcct tccacaagct gctgtgggga cgtgtgttcc
     3541 ctcaaagtct gtgccatctt ctcccacccc tcccgggtag aaggaggggc tgaccccagg
     3601 gctgggagag gggaggggac tggagggcag actggcttct cggtccccag ggggccgctt
     3661 gggctgttgg tctccagagc agggccactg ggcactctgt gatgggggag cctttgtctg
     3721 aaagcacagc cccctcgccc ttcctctccc catggcttcc ccttcattgg cattaatctg
     3781 ggcaccagct ctctccatag cagtgacttc cctcaccact ctcatctctc agccttgcct
     3841 tttcttcctg acactgtcgc cccctcctct caggagacac tgccgagggc cacctggcag
     3901 aaggctgagt taggcagcag ggccgggagc gtctgccctc cacagggtgg gggacagata
     3961 ggctaagcga ctcccagctt gctaccctca gtggccagtg tgggcgtggg cggtttgggg
     4021 cgcttggctg gtggtggcca ctgcatccct taatttattt ctctgctgtt tctgttcttg
     4081 agaaattggg ggtgggagtc ctacacagag gctgccccta ccctcacctg agttgtacat
     4141 ttttttgtga tgggttttat tttttattat tttattttat tttttttttt tttgatttat
     4201 gatgactcca cccctcttca tcacccccgc tcccaggcca ggctcagcga ttaagccgag
     4261 cccttgcgtc ctaggaaggg gccttgccaa cctcagccct cctgccccac actcctactg
     4321 cggctcagac caagggctcc ccctccctcc cttcccccct cctgccctat ggaacagccc
     4381 gggtgctctg agggggctgg gagggcatgg cttggctccc aaagggggta ggggcccggg
     4441 gcacccaggc aaggtggccc ctccccgtct agccccctcc tccccaaccc tgcacttagt
     4501 ttctcctctg gatcaaacac gtaataaaga gaatgtttgg aatctga
//
