LOCUS       NM_018650               5370 bp    mRNA    linear   PRI 06-APR-2024
DEFINITION  Homo sapiens microtubule affinity regulating kinase 1 (MARK1),
            transcript variant 2, mRNA.
ACCESSION   NM_018650
VERSION     NM_018650.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5370)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   2  (bases 1 to 5370)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   3  (bases 1 to 5370)
  AUTHORS   Tang,X., Yang,M., Wang,Z., Wu,X. and Wang,D.
  TITLE     MicroRNA-23a promotes colorectal cancer cell migration and
            proliferation by targeting at MARK1
  JOURNAL   Acta Biochim Biophys Sin (Shanghai) 51 (7), 661-668 (2019)
   PUBMED   31281935
  REMARK    GeneRIF: Microtubule affinity regulating kinase 1 (MARK1) is a
            direct target of miR-23a in colorectal cancer (CRC) cells.
REFERENCE   4  (bases 1 to 5370)
  AUTHORS   Emptage,R.P., Lemmon,M.A., Ferguson,K.M. and Marmorstein,R.
  TITLE     Structural Basis for MARK1 Kinase Autoinhibition by Its KA1 Domain
  JOURNAL   Structure 26 (8), 1137-1143 (2018)
   PUBMED   30099988
  REMARK    GeneRIF: Structural basis for MARK1 kinase autoinhibition by its
            KA1 domain has been uncovered.
REFERENCE   5  (bases 1 to 5370)
  AUTHORS   Natalia,M.A., Alejandro,G.T., Virginia,T.J. and Alvarez-Salas,L.M.
  TITLE     MARK1 is a Novel Target for miR-125a-5p: Implications for Cell
            Migration in Cervical Tumor Cells
  JOURNAL   Microrna 7 (1), 54-61 (2018)
   PUBMED   29076440
  REMARK    GeneRIF: Low MARK1 is associated with cervical cancer.
REFERENCE   6  (bases 1 to 5370)
  AUTHORS   Drewes,G., Ebneth,A., Preuss,U., Mandelkow,E.M. and Mandelkow,E.
  TITLE     MARK, a novel family of protein kinases that phosphorylate
            microtubule-associated proteins and trigger microtubule disruption
  JOURNAL   Cell 89 (2), 297-308 (1997)
   PUBMED   9108484
REFERENCE   7  (bases 1 to 5370)
  AUTHORS   Paudel,H.K.
  TITLE     The regulatory Ser262 of microtubule-associated protein tau is
            phosphorylated by phosphorylase kinase
  JOURNAL   J Biol Chem 272 (3), 1777-1785 (1997)
   PUBMED   8999860
REFERENCE   8  (bases 1 to 5370)
  AUTHORS   Illenberger,S., Drewes,G., Trinczek,B., Biernat,J., Meyer,H.E.,
            Olmsted,J.B., Mandelkow,E.M. and Mandelkow,E.
  TITLE     Phosphorylation of microtubule-associated proteins MAP2 and MAP4 by
            the protein kinase p110mark. Phosphorylation sites and regulation
            of microtubule dynamics
  JOURNAL   J Biol Chem 271 (18), 10834-10843 (1996)
   PUBMED   8631898
REFERENCE   9  (bases 1 to 5370)
  AUTHORS   Drewes,G., Trinczek,B., Illenberger,S., Biernat,J.,
            Schmitt-Ulms,G., Meyer,H.E., Mandelkow,E.M. and Mandelkow,E.
  TITLE     Microtubule-associated protein/microtubule affinity-regulating
            kinase (p110mark). A novel protein kinase that regulates
            tau-microtubule interactions and dynamic instability by
            phosphorylation at the Alzheimer-specific site serine 262
  JOURNAL   J Biol Chem 270 (13), 7679-7688 (1995)
   PUBMED   7706316
REFERENCE   10 (bases 1 to 5370)
  AUTHORS   Yang,S.D., Yu,J.S., Shiah,S.G. and Huang,J.J.
  TITLE     Protein kinase FA/glycogen synthase kinase-3 alpha after heparin
            potentiation phosphorylates tau on sites abnormally phosphorylated
            in Alzheimer's disease brain
  JOURNAL   J Neurochem 63 (4), 1416-1425 (1994)
   PUBMED   7931292
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC096640.2, BC068608.1, BC113869.1, AL592406.4 and CA412112.1.
            
            On Jun 2, 2019 this sequence version replaced NM_018650.4.
            
            Transcript Variant: This variant (2) uses an alternate in-frame
            splice site compared to variant 1. The encoded isoform (2) is
            shorter than isoform 1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC114478.1, SRR14038196.2184107.1
                                           [ECO:0000332]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000366917.6/ ENSP00000355884.5
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-47                AC096640.2         69421-69467
            48-511              BC068608.1         1-464
            512-3321            BC113869.1         1-2810
            3322-5128           AL592406.4         38358-40164
            5129-5366           CA412112.1         18-255              c
            5367-5370           AL592406.4         40403-40406
FEATURES             Location/Qualifiers
     source          1..5370
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1q41"
     gene            1..5370
                     /gene="MARK1"
                     /gene_synonym="MARK; Par-1c; Par1c"
                     /note="microtubule affinity regulating kinase 1"
                     /db_xref="GeneID:4139"
                     /db_xref="HGNC:HGNC:6896"
                     /db_xref="MIM:606511"
     exon            1..738
                     /gene="MARK1"
                     /gene_synonym="MARK; Par-1c; Par1c"
                     /inference="alignment:Splign:2.1.0"
     CDS             688..3075
                     /gene="MARK1"
                     /gene_synonym="MARK; Par-1c; Par1c"
                     /EC_number="2.7.11.1"
                     /EC_number="2.7.11.26"
                     /note="isoform 2 is encoded by transcript variant 2;
                     serine/threonine-protein kinase MARK1; PAR1 homolog c;
                     MAP/microtubule affinity-regulating kinase 1"
                     /codon_start=1
                     /product="serine/threonine-protein kinase MARK1 isoform 2"
                     /protein_id="NP_061120.3"
                     /db_xref="CCDS:CCDS31029.2"
                     /db_xref="GeneID:4139"
                     /db_xref="HGNC:HGNC:6896"
                     /db_xref="MIM:606511"
                     /translation="MSARTPLPTVNERDTENHTSVDGYTEPHIQPTKSSSRQNIPRCR
                     NSITSATDEQPHIGNYRLQKTIGKGNFAKVKLARHVLTGREVAVKIIDKTQLNPTSLQ
                     KLFREVRIMKILNHPNIVKLFEVIETEKTLYLVMEYASGGEVFDYLVAHGRMKEKEAR
                     AKFRQIVSAVQYCHQKYIVHRDLKAENLLLDGDMNIKIADFGFSNEFTVGNKLDTFCG
                     SPPYAAPELFQGKKYDGPEVDVWSLGVILYTLVSGSLPFDGQNLKELRERVLRGKYRI
                     PFYMSTDCENLLKKLLVLNPIKRGSLEQIMKDRWMNVGHEEEELKPYTEPDPDFNDTK
                     RIDIMVTMGFARDEINDALINQKYDEVMATYILLGRKPPEFEGGESLSSGNLCQRSRP
                     SSDLNNSTLQSPAHLKVQRSISANQKQRRFSDHAGPSIPPAVSYTKRPQANSVESEQK
                     EEWDKDVARKLGSTTVGSKSEMTASPLVGPERKKSSTIPSNNVYSGGSMARRNTYVCE
                     RTTDRYVALQNGKDSSLTEMSVSSISSAGSSVASAVPSARPRHQKSMSTSGHPIKVTL
                     PTIKDGSEAYRPGTTQRVPAASPSAHSISTATPDRTRFPRGSSSRSTFHGEQLRERRS
                     VAYNGPPASPSHETGAFAHARRGTSTGIISKITSKFVRRDPSEGEASGRTDTSRSTSG
                     EPKERDKEEGKDSKPRSLRFTWSMKTTSSMDPNDMMREIRKVLDANNCDYEQKERFLL
                     FCVHGDARQDSLVQWEMEVCKLPRLSLNGVRFKRISGTSIAFKNIASKIANELKL"
     exon            739..942
                     /gene="MARK1"
                     /gene_synonym="MARK; Par-1c; Par1c"
                     /inference="alignment:Splign:2.1.0"
     exon            943..996
                     /gene="MARK1"
                     /gene_synonym="MARK; Par-1c; Par1c"
                     /inference="alignment:Splign:2.1.0"
     exon            997..1045
                     /gene="MARK1"
                     /gene_synonym="MARK; Par-1c; Par1c"
                     /inference="alignment:Splign:2.1.0"
     exon            1046..1111
                     /gene="MARK1"
                     /gene_synonym="MARK; Par-1c; Par1c"
                     /inference="alignment:Splign:2.1.0"
     exon            1112..1182
                     /gene="MARK1"
                     /gene_synonym="MARK; Par-1c; Par1c"
                     /inference="alignment:Splign:2.1.0"
     exon            1183..1239
                     /gene="MARK1"
                     /gene_synonym="MARK; Par-1c; Par1c"
                     /inference="alignment:Splign:2.1.0"
     exon            1240..1476
                     /gene="MARK1"
                     /gene_synonym="MARK; Par-1c; Par1c"
                     /inference="alignment:Splign:2.1.0"
     exon            1477..1596
                     /gene="MARK1"
                     /gene_synonym="MARK; Par-1c; Par1c"
                     /inference="alignment:Splign:2.1.0"
     exon            1597..1696
                     /gene="MARK1"
                     /gene_synonym="MARK; Par-1c; Par1c"
                     /inference="alignment:Splign:2.1.0"
     exon            1697..1809
                     /gene="MARK1"
                     /gene_synonym="MARK; Par-1c; Par1c"
                     /inference="alignment:Splign:2.1.0"
     exon            1810..1963
                     /gene="MARK1"
                     /gene_synonym="MARK; Par-1c; Par1c"
                     /inference="alignment:Splign:2.1.0"
     exon            1964..2157
                     /gene="MARK1"
                     /gene_synonym="MARK; Par-1c; Par1c"
                     /inference="alignment:Splign:2.1.0"
     exon            2158..2258
                     /gene="MARK1"
                     /gene_synonym="MARK; Par-1c; Par1c"
                     /inference="alignment:Splign:2.1.0"
     exon            2259..2423
                     /gene="MARK1"
                     /gene_synonym="MARK; Par-1c; Par1c"
                     /inference="alignment:Splign:2.1.0"
     exon            2424..2675
                     /gene="MARK1"
                     /gene_synonym="MARK; Par-1c; Par1c"
                     /inference="alignment:Splign:2.1.0"
     exon            2676..2720
                     /gene="MARK1"
                     /gene_synonym="MARK; Par-1c; Par1c"
                     /inference="alignment:Splign:2.1.0"
     exon            2721..5370
                     /gene="MARK1"
                     /gene_synonym="MARK; Par-1c; Par1c"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3341..3346
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MARK1"
                     /gene_synonym="MARK; Par-1c; Par1c"
                     /note="hexamer: AATAAA"
     polyA_site      3367
                     /gene="MARK1"
                     /gene_synonym="MARK; Par-1c; Par1c"
     regulatory      4814..4819
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MARK1"
                     /gene_synonym="MARK; Par-1c; Par1c"
                     /note="hexamer: AATAAA"
     polyA_site      4840
                     /gene="MARK1"
                     /gene_synonym="MARK; Par-1c; Par1c"
                     /note="major polyA site"
     regulatory      5259..5264
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MARK1"
                     /gene_synonym="MARK; Par-1c; Par1c"
                     /note="hexamer: AATAAA"
     polyA_site      5285
                     /gene="MARK1"
                     /gene_synonym="MARK; Par-1c; Par1c"
     regulatory      5347..5352
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MARK1"
                     /gene_synonym="MARK; Par-1c; Par1c"
                     /note="hexamer: ATTAAA"
     polyA_site      5370
                     /gene="MARK1"
                     /gene_synonym="MARK; Par-1c; Par1c"
ORIGIN      
        1 acccggcggc ggccgccaag tgcctctggg cgctgcgtgc cgcgcccgct gctccgcgcg
       61 cagccggctc gggccgctcc tcctgactga ggcgcggcgg cggcggtggc tgtgaccgcg
      121 cggaccgagc cgagacattc gcgccggggg atcgggcgcc gccgccgctg ggccccgggc
      181 gcgtggatgc ggctgggtcg ggcggcgccg tacacctgag gcggagaacg gggcgcggcg
      241 cgggtgacgc tgtcagggcc gcggttcctg acgcccaggc gctcgccagg acgagccagg
      301 cagtgatttg aggcaccggc ttcaccttca cccatggtcc ggagagccta gcggggctcg
      361 ccaccgcctc ccggctcccc ttccacgcct catcctgcca gcctcgccgc cccgccagcg
      421 ccgggcaacc gcctcgcccg aagccctccc tcgttactgt ccgcataccc cggcggcgcc
      481 gccgcgggaa gcggctcccc ctcctcttcc tccgcgtcct cttccctctt tcccccgccg
      541 gggccgcttg ttgcaccgcc ccgcggcctg cgggagccgc tcgccccggc cttgtgctcg
      601 cgtccgcacc cctttcctgt cgccccccgg ggcccgcacc acagcccggc cggcgagacc
      661 ccggccagac cccgctgccc gcacaaaatg tcggcccgga cgccattgcc gacggtgaac
      721 gagcgggaca cggaaaatca tacatctgtg gatggatata ctgaaccaca catccagcct
      781 accaagtcga gtagcagaca gaacatcccc cggtgtagaa actccattac gtcagcaaca
      841 gatgaacagc ctcacattgg aaattaccgt ttacaaaaaa caatagggaa gggaaatttt
      901 gccaaagtca aattggcaag acacgttcta actggtagag aggttgctgt gaaaataata
      961 gacaaaactc agctaaatcc taccagtcta caaaagttat ttcgagaagt acgaataatg
     1021 aagatactga atcatcctaa tatagtaaaa ttgtttgaag ttattgaaac agagaagact
     1081 ctctatttag tcatggaata cgcgagtggg ggtgaagtat ttgattactt agttgcccat
     1141 ggaagaatga aagagaaaga ggcccgtgca aaatttaggc agattgtatc tgctgtacag
     1201 tattgtcatc aaaagtacat tgttcaccgt gatcttaagg ctgaaaacct tctccttgat
     1261 ggtgatatga atattaaaat tgctgacttt ggttttagta atgaatttac agttgggaac
     1321 aaattggaca cattttgtgg aagcccaccc tatgctgctc ccgagctttt ccaaggaaag
     1381 aagtatgatg ggcctgaagt ggatgtgtgg agtctgggcg tcattctcta tacattagtc
     1441 agtggctcct tgcctttcga tggccagaat ttaaaggaac tgcgagagcg agttttacga
     1501 gggaagtacc gtattccctt ctatatgtcc acagactgtg aaaatcttct gaagaaatta
     1561 ttagtcctga atccaataaa gagaggcagc ttggaacaaa taatgaaaga tcgatggatg
     1621 aatgttggtc atgaagagga agaactaaag ccatatactg agcctgatcc ggatttcaat
     1681 gacacaaaaa gaatagacat tatggtcacc atgggctttg cacgagatga aataaatgat
     1741 gccttaataa atcagaagta tgatgaagtt atggctactt atattcttct aggtagaaaa
     1801 ccacctgaat ttgaaggtgg tgaatcgtta tccagtggaa acttgtgtca gaggtcccgg
     1861 cccagtagtg acttaaacaa cagcactctt cagtcccctg ctcacctgaa ggtccagaga
     1921 agtatctcag caaatcagaa gcagcggcgt ttcagtgatc atgctggtcc atccattcct
     1981 cctgctgtat catataccaa aagacctcag gctaacagtg tggaaagtga acagaaagag
     2041 gagtgggaca aagatgtggc tcgaaaactt ggcagcacaa cagttggatc aaaaagcgag
     2101 atgactgcaa gccctcttgt agggccagag aggaaaaaat cttcaactat tccaagtaac
     2161 aatgtgtatt ctggaggtag catggcaaga aggaatacat atgtctgtga aaggaccaca
     2221 gatcgatacg tagcattgca gaatggaaaa gacagcagcc ttacggagat gtctgtgagt
     2281 agcatatctt ctgcaggctc ttctgtggcc tctgctgtcc cctcagcacg accccgccac
     2341 cagaagtcca tgtccacttc tggtcatcct attaaagtca cactgccaac cattaaagac
     2401 ggctctgaag cttaccggcc tggtacaacc cagagagtgc ctgctgcttc cccatctgct
     2461 cacagtatta gtactgcgac tccagaccgg acccgttttc cccgagggag ctcaagccga
     2521 agcactttcc atggtgaaca gctccgggag cgacgcagcg ttgcttataa tgggccacct
     2581 gcttcaccat cccatgaaac gggtgcattt gcacatgcca gaaggggaac gtcaactggt
     2641 ataataagca aaatcacatc caaatttgtt cgcagggatc caagtgaagg cgaagccagt
     2701 ggcagaaccg acacctcaag aagtacatca ggggaaccaa aagaaagaga caaggaagag
     2761 ggtaaagatt ctaagccgcg ttctttgcgg ttcacatgga gtatgaagac cactagttca
     2821 atggacccta atgacatgat gagagaaatc cgaaaagtgt tagatgcaaa taactgtgat
     2881 tatgagcaaa aagagagatt tttgcttttc tgtgtccatg gagacgctag acaggatagc
     2941 ctcgtgcagt gggagatgga agtctgcaag ttgccacgac tgtcacttaa tggggttcgc
     3001 ttcaagcgaa tatctgggac atctattgcc tttaagaaca ttgcatcaaa aatagcaaat
     3061 gagcttaagc tgtaaagaag tccaaattta caggttcagg gaagatacat acatatatga
     3121 ggtacagttt ttgaatgtac tggtaatgcc taatgtggtc tgcctgtgaa tctccccatg
     3181 tagaatttgc ccttaatgca ataaggttat acatagttat gaactgtaaa attaaagtca
     3241 gtatgaacta taataaatat ctgtagctta aaaagtaggt tcacatgtac aggtaagtat
     3301 attgtgtatt tctgttcatt ttctgttcat agagttgtat aataaaacat gattgcttaa
     3361 aaacttgtat agttgtctag atttctgcac ctgaatgtat gtttgatgct ttgatttgaa
     3421 aatgttcttc cctgttattt acattctggt gggtttttaa aattcttacc tccatcatgc
     3481 aattttgaaa attgtgtcca gaattaaaag tgcatagaaa tagcctttac aattgtagca
     3541 tggaccttta aaaattgttt taaaatctta tttaaattta aaccagaagc tgaaaaatag
     3601 atcagcttta ttatacacaa aattattact gcttatcttt gctcttttcc ttgttatccc
     3661 gcaaggttta gttgagaaga tacaaaatgt ttacagtgtt ggcacttaga gtttttaaat
     3721 tcaagtacat gaaattcagt aatagcattg ccttgagcta actaggaagt accgggaaaa
     3781 aagttaaatc tacatcaagt ttcttttgaa ctttgaagtg ttttctgacc cactgctaac
     3841 tgtagcaaca aaatttaaaa gaaaaaaaac atactttatc tggctattat aacataaact
     3901 gtcacgtagg tttgctgcct tcagaatacc gcaatttaat tgcgggaata taataatatt
     3961 gggactgttt cacagcacaa actcatcttt acagtgttga tcaatgcatc agttaagaaa
     4021 taatgccacc tcaggaatta actggcattg ggaacatttg cctcattctc ctgctatcct
     4081 cttcattcac ccctgccact gtaatatcta taagtactta agagacttgt gagcaaaaca
     4141 tactatttat aacagtatat gattgattta tgcttatgtg gttgttcagt ttgttcccat
     4201 gtaactcgtt tgttttaaat attttgccag atttcttgta tttattccac atcattatgc
     4261 ctataatgtg ccgctttgtg attgggcatt tgcctacttt tctttcataa ttagtgatat
     4321 atgcgatgta aaaccactag taaaggtaca ttttaatact tgttatttta tactgaatta
     4381 gccttggagg ttgactgtgc aatgttattt actgttgtaa ttactgtaat accaacatat
     4441 gggccccatc tgcacactcc tgaaaaacag aaagtgtatt caaattttat cagtttaaag
     4501 aaaataaagc tgtgataaat actgtaattc caacctacat tagaaggtct aagtgtaggt
     4561 gatgtgccat tccataatgg cttccagact agggtgaatt ttatgttctg tactgtactg
     4621 tgatgtagct ttcttctgta acagttatgt tttaaaatta agtgagtttt ttttttgcct
     4681 tagcaaaggg tggtgtttga aaaaaaaaat gtgtagcccc tttttaacct agtgttcatt
     4741 caaaaaaaaa ttgatgcaaa tctttattca ctttcactgg tgcacactga aattttactt
     4801 gaacagttct cataataaag cacttgtctt ttgctcttta tcagaatgtg aattacctgt
     4861 tttctggtac aaaagtattc tgtatgagga gtttattgta tgtgttctaa atttagttgg
     4921 caaagggtga agctgtgaag gttttcaaga ttattgaaac tatgaaggtt tcttgtcatt
     4981 atgacaagaa agtttaatct ttttatagga attcctgtca ctgaaatacg tttttaaaaa
     5041 aatagactca tgtgttttcc acggtagaaa ctgatatttt tttacatttt ctcactgtgg
     5101 ccaactcttc tgtgtttgta gaaaggaatt tgacttcaat atcttttatg aactaaaaat
     5161 gaaatcttga tactcacttt agatttttca ttttatgtgt tcatgacaac ataaatattt
     5221 ttcaaagatt tagaggaatt ttgcaatgtg tttgcataaa taaataccag tttatgttca
     5281 ccggctatgt gataccagga tttccttggc ttctgttgaa atattatttg atatgacatc
     5341 ccttatatta aattaattat tttgtagaaa
//
