LOCUS       NM_001282866            5615 bp    mRNA    linear   PRI 06-AUG-2024
DEFINITION  Homo sapiens membrane associated ring-CH-type finger 8 (MARCHF8),
            transcript variant 4, mRNA.
ACCESSION   NM_001282866 XM_005271805
VERSION     NM_001282866.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5615)
  AUTHORS   Rao,C.H., Huang,R., Bai,Y.Z., Yu,C., Chen,M., Peng,J.M., Xu,S.J.,
            Sun,M.X., Wang,S.J., Yang,Y.B., An,T.Q., Tian,Z.J., Lyu,C.,
            Cai,X.H., Zheng,C., Meng,F. and Tang,Y.D.
  TITLE     MARCH8 inhibits pseudorabies virus replication by trapping the
            viral cell-to-cell fusion complex in the trans-Golgi network
  JOURNAL   Int J Biol Macromol 274 (Pt 2), 133463 (2024)
   PUBMED   38944094
  REMARK    GeneRIF: MARCH8 inhibits pseudorabies virus replication by trapping
            the viral cell-to-cell fusion complex in the trans-Golgi network.
REFERENCE   2  (bases 1 to 5615)
  AUTHORS   Khalil,M.I., Yang,C., Vu,L., Chadha,S., Nabors,H., James,C.D.,
            Morgan,I.M. and Pyeon,D.
  TITLE     The membrane-associated ubiquitin ligase MARCHF8 stabilizes the
            human papillomavirus oncoprotein E7 by degrading CUL1 and UBE2L3 in
            head and neck cancer
  JOURNAL   J Virol 98 (2), e0172623 (2024)
   PUBMED   38226814
  REMARK    GeneRIF: The membrane-associated ubiquitin ligase MARCHF8
            stabilizes the human papillomavirus oncoprotein E7 by degrading
            CUL1 and UBE2L3 in head and neck cancer.
REFERENCE   3  (bases 1 to 5615)
  AUTHORS   Yu,C., Bai,Y., Tan,W., Bai,Y., Li,X., Zhou,Y., Zhai,J., Xue,M.,
            Tang,Y.D., Zheng,C. and Liu,Q.
  TITLE     Human MARCH1, 2, and 8 block Ebola virus envelope glycoprotein
            cleavage via targeting furin P domain
  JOURNAL   J Med Virol 96 (2), e29445 (2024)
   PUBMED   38299743
  REMARK    GeneRIF: Human MARCH1, 2, and 8 block Ebola virus envelope
            glycoprotein cleavage via targeting furin P domain.
REFERENCE   4  (bases 1 to 5615)
  AUTHORS   Zhao,Y., Sui,L., Wu,P., Li,L., Liu,L., Ma,B., Wang,W., Chi,H.,
            Wang,Z.-D., Wei,Z., Hou,Z., Zhang,K., Niu,J., Jin,N., Li,C.,
            Zhao,J., Wang,G. and Liu,Q.
  TITLE     EGR1 functions as a new host restriction factor for SARS-CoV-2 to
            inhibit virus replication through the E3 ubiquitin ligase MARCH8
  JOURNAL   J Virol 97 (10), e0102823 (2023)
   PUBMED   37772822
  REMARK    GeneRIF: EGR1 functions as a new host restriction factor for
            SARS-CoV-2 to inhibit virus replication through the E3 ubiquitin
            ligase MARCH8.
REFERENCE   5  (bases 1 to 5615)
  AUTHORS   Chen,C., Wang,Y., Zhao,Q., Li,G.D., Wang,Y.H., Xu,L.C., Huang,H.Z.,
            Song,G., Li,W.T. and He,X.H.
  TITLE     E3 Ubiquitin Ligase MARCH8 Promotes Pancreatic Cancer Growth and
            Metastasis by Activating STAT3 via Degradation of PTPN4
  JOURNAL   Pancreas 52 (4), e224-e234 (2023)
   PUBMED   37747937
  REMARK    GeneRIF: E3 Ubiquitin Ligase MARCH8 Promotes Pancreatic Cancer
            Growth and Metastasis by Activating STAT3 via Degradation of PTPN4.
REFERENCE   6  (bases 1 to 5615)
  AUTHORS   Girard,A., Sachidanandam,R., Hannon,G.J. and Carmell,M.A.
  TITLE     A germline-specific class of small RNAs binds mammalian Piwi
            proteins
  JOURNAL   Nature 442 (7099), 199-202 (2006)
   PUBMED   16751776
REFERENCE   7  (bases 1 to 5615)
  AUTHORS   Grupe,A., Li,Y., Rowland,C., Nowotny,P., Hinrichs,A.L., Smemo,S.,
            Kauwe,J.S., Maxwell,T.J., Cherny,S., Doil,L., Tacey,K., van
            Luchene,R., Myers,A., Wavrant-De Vrieze,F., Kaleem,M.,
            Hollingworth,P., Jehu,L., Foy,C., Archer,N., Hamilton,G.,
            Holmans,P., Morris,C.M., Catanese,J., Sninsky,J., White,T.J.,
            Powell,J., Hardy,J., O'Donovan,M., Lovestone,S., Jones,L.,
            Morris,J.C., Thal,L., Owen,M., Williams,J. and Goate,A.
  TITLE     A scan of chromosome 10 identifies a novel locus showing strong
            association with late-onset Alzheimer disease
  JOURNAL   Am J Hum Genet 78 (1), 78-88 (2006)
   PUBMED   16385451
  REMARK    GeneRIF: Observational study of gene-disease association. (HuGE
            Navigator)
REFERENCE   8  (bases 1 to 5615)
  AUTHORS   Deloukas,P., Earthrowl,M.E., Grafham,D.V., Rubenfield,M.,
            French,L., Steward,C.A., Sims,S.K., Jones,M.C., Searle,S.,
            Scott,C., Howe,K., Hunt,S.E., Andrews,T.D., Gilbert,J.G.,
            Swarbreck,D., Ashurst,J.L., Taylor,A., Battles,J., Bird,C.P.,
            Ainscough,R., Almeida,J.P., Ashwell,R.I., Ambrose,K.D.,
            Babbage,A.K., Bagguley,C.L., Bailey,J., Banerjee,R., Bates,K.,
            Beasley,H., Bray-Allen,S., Brown,A.J., Brown,J.Y., Burford,D.C.,
            Burrill,W., Burton,J., Cahill,P., Camire,D., Carter,N.P.,
            Chapman,J.C., Clark,S.Y., Clarke,G., Clee,C.M., Clegg,S., Corby,N.,
            Coulson,A., Dhami,P., Dutta,I., Dunn,M., Faulkner,L., Frankish,A.,
            Frankland,J.A., Garner,P., Garnett,J., Gribble,S., Griffiths,C.,
            Grocock,R., Gustafson,E., Hammond,S., Harley,J.L., Hart,E.,
            Heath,P.D., Ho,T.P., Hopkins,B., Horne,J., Howden,P.J., Huckle,E.,
            Hynds,C., Johnson,C., Johnson,D., Kana,A., Kay,M., Kimberley,A.M.,
            Kershaw,J.K., Kokkinaki,M., Laird,G.K., Lawlor,S., Lee,H.M.,
            Leongamornlert,D.A., Laird,G., Lloyd,C., Lloyd,D.M., Loveland,J.,
            Lovell,J., McLaren,S., McLay,K.E., McMurray,A.,
            Mashreghi-Mohammadi,M., Matthews,L., Milne,S., Nickerson,T.,
            Nguyen,M., Overton-Larty,E., Palmer,S.A., Pearce,A.V., Peck,A.I.,
            Pelan,S., Phillimore,B., Porter,K., Rice,C.M., Rogosin,A.,
            Ross,M.T., Sarafidou,T., Sehra,H.K., Shownkeen,R., Skuce,C.D.,
            Smith,M., Standring,L., Sycamore,N., Tester,J., Thorpe,A.,
            Torcasso,W., Tracey,A., Tromans,A., Tsolas,J., Wall,M., Walsh,J.,
            Wang,H., Weinstock,K., West,A.P., Willey,D.L., Whitehead,S.L.,
            Wilming,L., Wray,P.W., Young,L., Chen,Y., Lovering,R.C.,
            Moschonas,N.K., Siebert,R., Fechtel,K., Bentley,D., Durbin,R.,
            Hubbard,T., Doucette-Stamm,L., Beck,S., Smith,D.R. and Rogers,J.
  TITLE     The DNA sequence and comparative analysis of human chromosome 10
  JOURNAL   Nature 429 (6990), 375-381 (2004)
   PUBMED   15164054
REFERENCE   9  (bases 1 to 5615)
  AUTHORS   Bartee,E., Mansouri,M., Hovey Nerenberg,B.T., Gouveia,K. and
            Fruh,K.
  TITLE     Downregulation of major histocompatibility complex class I by human
            ubiquitin ligases related to viral immune evasion proteins
  JOURNAL   J Virol 78 (3), 1109-1120 (2004)
   PUBMED   14722266
REFERENCE   10 (bases 1 to 5615)
  AUTHORS   Goto,E., Ishido,S., Sato,Y., Ohgimoto,S., Ohgimoto,K.,
            Nagano-Fujii,M. and Hotta,H.
  TITLE     c-MIR, a human E3 ubiquitin ligase, is a functional homolog of
            herpesvirus proteins MIR1 and MIR2 and has similar activity
  JOURNAL   J Biol Chem 278 (17), 14657-14668 (2003)
   PUBMED   12582153
  REMARK    GeneRIF: c-MIR induced specific down-regulation of B7-2 surface
            expression through ubiquitination, rapid endocytosis, and lysosomal
            degradation
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK307667.1, AK023636.1, BM151615.1, AL833316.1 and AK025472.1.
            
            On Nov 23, 2018 this sequence version replaced NM_001282866.1.
            
            Summary: MARCH8 is a member of the MARCH family of membrane-bound
            E3 ubiquitin ligases (EC 6.3.2.19). MARCH enzymes add ubiquitin
            (see MIM 191339) to target lysines in substrate proteins, thereby
            signaling their vesicular transport between membrane compartments.
            MARCH8 induces the internalization of several membrane
            glycoproteins (Goto et al., 2003 [PubMed 12582153]; Bartee et al.,
            2004 [PubMed 14722266]).[supplied by OMIM, Apr 2010].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK307667.1, ERR279837.6921.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000453424.7/ ENSP00000411848.2
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1893              AK307667.1         24-1916
            1894-2689           AK023636.1         1360-2155
            2690-3278           BM151615.1         1-589               c
            3279-5613           AL833316.1         2687-5021
            5614-5615           AK025472.1         2285-2286
FEATURES             Location/Qualifiers
     source          1..5615
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="10"
                     /map="10q11.21-q11.22"
     gene            1..5615
                     /gene="MARCHF8"
                     /gene_synonym="c-MIR; CMIR; MARCH-VIII; MARCH8; MIR;
                     RNF178"
                     /note="membrane associated ring-CH-type finger 8"
                     /db_xref="GeneID:220972"
                     /db_xref="HGNC:HGNC:23356"
                     /db_xref="MIM:613335"
     exon            1..161
                     /gene="MARCHF8"
                     /gene_synonym="c-MIR; CMIR; MARCH-VIII; MARCH8; MIR;
                     RNF178"
                     /inference="alignment:Splign:2.1.0"
     exon            162..341
                     /gene="MARCHF8"
                     /gene_synonym="c-MIR; CMIR; MARCH-VIII; MARCH8; MIR;
                     RNF178"
                     /inference="alignment:Splign:2.1.0"
     CDS             240..1961
                     /gene="MARCHF8"
                     /gene_synonym="c-MIR; CMIR; MARCH-VIII; MARCH8; MIR;
                     RNF178"
                     /EC_number="2.3.2.27"
                     /note="isoform b is encoded by transcript variant 4;
                     c-mir, cellular modulator of immune recognition;
                     membrane-associated RING-CH protein VIII; E3
                     ubiquitin-protein ligase MARCH8; cellular modulator of
                     immune recognition (c-MIR); RING finger protein 178;
                     membrane-associated RING finger protein 8; membrane
                     associated ring finger 8; membrane-associated ring finger
                     (C3HC4) 8, E3 ubiquitin protein ligase; RING-type E3
                     ubiquitin transferase MARCH8; RING-type E3 ubiquitin
                     transferase MARCHF8; E3 ubiquitin-protein ligase MARCHF8"
                     /codon_start=1
                     /product="E3 ubiquitin-protein ligase MARCHF8 isoform b"
                     /protein_id="NP_001269795.1"
                     /db_xref="CCDS:CCDS60519.1"
                     /db_xref="GeneID:220972"
                     /db_xref="HGNC:HGNC:23356"
                     /db_xref="MIM:613335"
                     /translation="MSMPLHQISAIPSQDAISARVYRSKTKEKEREEQNEKTLGHFMS
                     HSSNISKAGSPPSASAPAPVSSFSRTSITPSSQDICSSSAVFSECCHHSSVQSAVVSK
                     APHCQSSLTQGLTVTVICKDTLQASKRNSFGSEWAQALKPAKNTKARRTLKFSRSLND
                     VGEKAQDTSESFAYVERTCSEGKLILPQDTCLRTNRFHHKEKRTLNHKPLGNSKHSCV
                     SCLSAGRSTASEVEAGKGGRPGLLLEEKADGEATSRSRQLLQYLFSLSHGLSASSLHR
                     FHELESCAARLHTAKSSSGLAGSMGFCSDEMGDDDVFEDSTSAKLKSRVLRAPLCSTE
                     KDSDLDCPSPFSEKLPPISPVSTSGDVCRICHCEGDDESPLITPCHCTGSLHFVHQAC
                     LQQWIKSSDTRCCELCKYEFIMETKLKPLRKWEKLQMTSSERRKIMCSVTFHVIAITC
                     VVWSLYVLIDRTAEEIKQGQATGILEWPFWTKLVVVAIGFTGGLLFMYVQCKVYVQLW
                     KRLKAYNRVIYVQNCPETSKKNIFEKSPLTEPNFENKHGYGICHSDTNSSCCTEPEDT
                     GAEIIHV"
     exon            342..392
                     /gene="MARCHF8"
                     /gene_synonym="c-MIR; CMIR; MARCH-VIII; MARCH8; MIR;
                     RNF178"
                     /inference="alignment:Splign:2.1.0"
     exon            393..481
                     /gene="MARCHF8"
                     /gene_synonym="c-MIR; CMIR; MARCH-VIII; MARCH8; MIR;
                     RNF178"
                     /inference="alignment:Splign:2.1.0"
     exon            482..1327
                     /gene="MARCHF8"
                     /gene_synonym="c-MIR; CMIR; MARCH-VIII; MARCH8; MIR;
                     RNF178"
                     /inference="alignment:Splign:2.1.0"
     exon            1328..1508
                     /gene="MARCHF8"
                     /gene_synonym="c-MIR; CMIR; MARCH-VIII; MARCH8; MIR;
                     RNF178"
                     /inference="alignment:Splign:2.1.0"
     exon            1509..1656
                     /gene="MARCHF8"
                     /gene_synonym="c-MIR; CMIR; MARCH-VIII; MARCH8; MIR;
                     RNF178"
                     /inference="alignment:Splign:2.1.0"
     exon            1657..5615
                     /gene="MARCHF8"
                     /gene_synonym="c-MIR; CMIR; MARCH-VIII; MARCH8; MIR;
                     RNF178"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5592..5597
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MARCHF8"
                     /gene_synonym="c-MIR; CMIR; MARCH-VIII; MARCH8; MIR;
                     RNF178"
                     /note="hexamer: AATAAA"
     polyA_site      5615
                     /gene="MARCHF8"
                     /gene_synonym="c-MIR; CMIR; MARCH-VIII; MARCH8; MIR;
                     RNF178"
                     /note="major polyA site"
ORIGIN      
        1 atggtaggta tagggctgtg gatatcgtca gtttgaccaa gtattccaag aggccccatg
       61 gagtttgtca tctgtaatag aggacgttag tgcattagtg atgaaaaaac tgcttaccac
      121 tgactattgt gatctcccag ggagtataag gcagctccgc acttgaaatc catggcccaa
      181 aatgactcta ccagtggaga ctctcttctg tgaagaagac gaccagataa gaggttggga
      241 tgagcatgcc actgcatcag atctctgcca ttccatccca ggatgccatc tctgctagag
      301 tctacagaag taagaccaaa gaaaaggaga gggaagaaca gaatgagaag actttgggac
      361 atttcatgag tcattcaagc aacatttcta aggctgggag tcctccgtca gcatcagctc
      421 cggctccggt gtcctccttc tctcgcactt ctatcacgcc atccagccag gacatctgca
      481 gttccagtgc agtgttttct gagtgttgtc accacagttc cgtgcagtct gctgttgtct
      541 cgaaagctcc tcactgccag agttctctga cacaagggct cactgtgaca gttatctgta
      601 aggacacatt acaggcgtca aagagaaatt cctttggttc agaatgggcc caggccttga
      661 agcctgctaa gaataccaaa gccagaagaa cactaaagtt ctcaaggtcc ctcaatgatg
      721 tgggtgagaa ggcgcaggat acttcagaaa gttttgccta tgtggaaaga acttgttctg
      781 aagggaaatt aatactccct caagatacgt gtctcagaac taacaggttt catcataaag
      841 aaaaaagaac cctgaaccac aaacctcttg gcaattccaa acattcttgt gtttcatgcc
      901 tttctgccgg tcgctcaact gcctcagagg tggaagctgg caaggggggc aggcccggcc
      961 tgctgctgga agagaaggcg gatggtgagg ccacgtcccg aagccggcaa ctgctccagt
     1021 acctgttctc actctcgcac ggcttgagcg ccagcagcct gcacaggttc catgagctgg
     1081 agagctgcgc tgctcgcctg cacactgcca agtcctccag cgggctggca gggagtatgg
     1141 gcttctgctc tgacgagatg ggagacgacg atgtctttga ggacagcaca tctgcaaaac
     1201 tgaagagtag ggttctgcgg gcgcccctct gctccacgga aaaggacagc gacctggatt
     1261 gtccttctcc cttctctgaa aaattacccc ccatatctcc cgtgtccacg tcaggggatg
     1321 tctgcaggat ctgccactgt gaaggagatg atgagagccc cctgatcacc ccctgccact
     1381 gcacaggaag cctccacttc gtgcaccagg cctgcctgca gcagtggatc aagagctccg
     1441 acacgcgctg ctgcgagctc tgcaagtatg agttcatcat ggagaccaag ctgaagccac
     1501 tgagaaaatg ggagaagttg cagatgacgt ccagcgagcg caggaagatc atgtgctcag
     1561 tgacattcca cgtcattgcc atcacatgtg tggtctggtc cttgtatgtg ctcattgacc
     1621 gtactgctga ggagatcaag caggggcagg caacaggaat cctagaatgg cccttttgga
     1681 ctaaattggt ggttgtggcc atcggcttca ccggaggact tctttttatg tatgttcagt
     1741 gtaaagtgta tgtgcaattg tggaagagac tcaaggccta taatagagtg atctatgttc
     1801 aaaactgtcc agaaacaagc aaaaagaata tttttgaaaa atctccacta acagagccca
     1861 actttgaaaa taaacatgga tatggaatct gtcattccga cacaaactct tcttgttgca
     1921 cagagcctga agacactgga gcagaaatca ttcacgtctg attgtgtgcg ggttgtcatt
     1981 ttcctggaca tccatgaaga gctgaaggaa attgtttact gccaattgta tacctttctt
     2041 atgtccttta atagcataga ctggacaggt gactatttat agtggcttct ctttttctaa
     2101 accctcctta gtctcctaga aaaccttcct gtgggccagg catgcctggg tcctgcctct
     2161 gcctggcagc tctgtgggaa agtggaagac cccatgatga catcatgggg agccagcaga
     2221 gttcctgccc atggtcttga gctgaatgag agaataaaat gccaatccca agggaagagg
     2281 aggagcaggg gtgcccaggc cctgataccc agccgcctcc agcttgcagt ggtccccagc
     2341 ctggagcaga gcattgggga gtgtctagcc atgacgagaa gattccctct gcatcacggc
     2401 gaaccccagg agatggtatt gaaacagacc cccaaacaca gactcctgcc tgccctctgc
     2461 cgatgctgcc tcctccatgc tcttgagcag gtggagccat ggtgctctgt ggtggcgcat
     2521 gattcactga gcaaacagca ctttacagaa gaaaatcttt attttgtaat atgtgtgtcc
     2581 agcgggattg acactcaaaa aaagtctcac ttagaaatct tcccttcctt acctttgtat
     2641 ctcctttaca tcatgagaga tcaaaaatcc attttgcctt acatatgcta agaagcgtgg
     2701 cattctttct gttcataaaa cagactcatg acttttaaga caggacaagc aggccagagg
     2761 cttttttttt aatcacagat ctcatgggaa ttagtggatg ctaaatggca attaaagcat
     2821 gattcctatg cttttgagcc tgttttatga caaggtgtta ctttgtagac ctggcccata
     2881 catttctttt ttgtcaacac tgaaaaagaa cagtttctga gatcgaaatg gaaggaaagg
     2941 aagtgagtaa tgacaacaaa actgaatatt taaaaaatat tcctttatag ttaaatctct
     3001 ccagttggtg ttggcttcac attgaaagag cagccatgtg ccaccctggc cggggtgtgt
     3061 gcactgcatg caggttctcc cttgcatgca agatgggtgc tggcccactg tgaggcttct
     3121 gctcggggaa gttctgtgga gtcactacca gggccaagag gcaggtgggc tggctctggg
     3181 acgagggcca ggtgtgagca aactgccagg gtccgctgtg ccactgctcc ccggccacat
     3241 gcccgcccag ctgtcgcctt aaccagactg aacagacagg atccttcagg gcccttggcc
     3301 ccactctcag tgcattctgt catcagggat taccacctgt accattttca taaaacaaaa
     3361 ccaaaatcac taccatcaaa tggccttgtc cctctaaaaa gtctgcttaa aattctgttt
     3421 ttaaaaccca gtttcctagt tttccaggca aatagttacc tccgggaaag ttgctggggg
     3481 ggcctgaagc acaatgtagc gcagatgctt cctttccagg ccattctctc acccagcctg
     3541 cacggaggag atgggagatg ctgggggtcc tgccctcagt ctttttgggc cttaggcgtt
     3601 tcgttcatcc tgctaagggg atgaagcaaa cacgaggtga ttcctttgcc tttcagagtg
     3661 gaagccctgg agtttgtttt gaaggccagg aggctgaagg atctctaagc tacggtgtgg
     3721 gcttaatagc agcaggcttt gtcctcctgt ctcctccaag ccagtgtctg attccttggc
     3781 aacacaggtc ttagtctgtg gagtggctct gctgtggcct tcctctggcc gggcaggcac
     3841 tgtccagcca tagccagctc ctgagaatag gtcagcctct cctttctgtc tcccagggca
     3901 catccagccc gtgcctgtgt tcactgtgcc ccgaagtgca attacccata ccccttctca
     3961 gcctggggac cccaggcaac cacagactgt ccactcaggg gagctgaatc ccaggtcagc
     4021 cctgccaatg tcccttagga actgcccagg caaggcccct ggttttgtat acttgttcct
     4081 gccacccagc agtagatgag tgtttcaggt gaagaccagg atagattttc taagtgtgaa
     4141 tccccacttc acatatggaa ccccttatgc tgaacttgaa aagcaccaag acttcctgta
     4201 gacaagaaag tgcttaggta gggacagccc ctgggcatcc cacccaatgt agctggcacc
     4261 ccactatggc aaaggtgcct tgataactga gccctgtatc cctcccatgc ccagccagat
     4321 tctcatggga agccctctcc cttcttttct gcctaacacc atctcatcgt ttctggcctc
     4381 actgtggaca atccacacac attcttcttt cctctcctgg cggggcacag agccaccccc
     4441 ttgccttttc ttttcttgaa ggttctagtt cagctcctga ttcatcagac ccttctagcc
     4501 ccctgcatct agcagtgaag catgaagcct ggtggggatg tggtactccc atctggtgtg
     4561 gccaccagct ctgccaatgt tcctgtagcc ttggaaaact tgctctctcg gttcttttgg
     4621 gtgctgtgta ctccccagct tccccccttc cccccccatt ttgcacctgg gtttagtgaa
     4681 aggatggcat ttggttgacc catatagaaa cccagaatga ggtctcaggg ccaggaggcc
     4741 tggtatttgt aggccaggga aggggaagag gcaagtggtc tggggtatca ccagccagcc
     4801 ctctctgatt tggcctctac tccccataag tcacagtacc ataagcaggc ttctggcctc
     4861 agcaatttgg tctttgtgcc caagtttatt gtgagaattt cctgaaaact ctataaaagg
     4921 tctcttccta ctgtaggcct ctaatgtttc tccccttttt gcttcagtcc actcttcagt
     4981 cttgtaggcc tagttttcaa acctgcacat gtgtcctacc tggccacagg catgcaggcc
     5041 tcaggcagct gggccagttt gggagcctcg ggtgatgtct gcacgatctg gggctgcctc
     5101 tgcacccctg ctgtgggctt cagggttgga gaagggctgg gaccaaccgg gtgagatcca
     5161 caagtctctg gatgtggctg aaggcaaata cacaattgaa gtactttctg ttttgaagtg
     5221 ctttcccttt tgaatctggt ttgaaacatg cagcttctgt ctctagccca aggaaagacc
     5281 aaaacatagg gaaataaaag catttatctt tgtcttggaa gtaattgttg aagttgtgca
     5341 gttgatcagt gcacagttag atgcaatgtt tatagaaatt gattgttaaa ccaaatttac
     5401 actggcatgt gtggtgtagt ttctaaaagg cacttcacat ttgaaatttt tcttacctta
     5461 gaaagtttct agtgatctaa atgtctagtt ttgtattctt ttgtgtgtgt tcactgtttc
     5521 tcagtattac cacttgaata attctctgta caggggggtt tgtgctatac actgggatgt
     5581 ctaattgcag caataaagcc tttctttaaa aagga
//
