LOCUS       NM_001282805            6043 bp    mRNA    linear   PRI 05-DEC-2024
DEFINITION  Homo sapiens membrane associated ring-CH-type finger 7 (MARCHF7),
            transcript variant 1, mRNA.
ACCESSION   NM_001282805
VERSION     NM_001282805.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6043)
  AUTHORS   Shi,X. and Zhang,X.
  TITLE     Control of ATG14 solubility and autophagy by
            MARCHF7/MARCH7-mediated ubiquitination
  JOURNAL   Autophagy 20 (3), 699-700 (2024)
   PUBMED   37915253
  REMARK    GeneRIF: Control of ATG14 solubility and autophagy by
            MARCHF7/MARCH7-mediated ubiquitination.
REFERENCE   2  (bases 1 to 6043)
  AUTHORS   Singh,S., Bano,A., Saraya,A., Das,P. and Sharma,R.
  TITLE     Association of MARCH7 with tumor progression and T-cell
            infiltration in esophageal cancer
  JOURNAL   Med Oncol 40 (1), 67 (2022)
   PUBMED   36583798
  REMARK    GeneRIF: Association of MARCH7 with tumor progression and T-cell
            infiltration in esophageal cancer.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 6043)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   4  (bases 1 to 6043)
  AUTHORS   Mahajan,S., Sharma,G.K., Bora,K. and Pattnaik,B.
  TITLE     Identification of novel interactions between host and
            non-structural protein 2C of foot-and-mouth disease virus
  JOURNAL   J Gen Virol 102 (3) (2021)
   PUBMED   33729124
  REMARK    GeneRIF: Identification of novel interactions between host and
            non-structural protein 2C of foot-and-mouth disease virus.
REFERENCE   5  (bases 1 to 6043)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   6  (bases 1 to 6043)
  AUTHORS   Muthukumarana,P.A., Lyons,G.E., Miura,Y., Thompson,L.H., Watson,T.,
            Green,C.J., Shurey,S., Hess,A.D., Rosengard,B.R. and Metcalfe,S.M.
  TITLE     Evidence for functional inter-relationships between FOXP3,
            leukaemia inhibitory factor, and axotrophin/MARCH-7 in
            transplantation tolerance
  JOURNAL   Int Immunopharmacol 6 (13-14), 1993-2001 (2006)
   PUBMED   17161353
  REMARK    GeneRIF: Data suggest that clinical auto-graft versus host disease
            inversely correlates with axotrophin transcript expression.
REFERENCE   7  (bases 1 to 6043)
  AUTHORS   Flierman,D., Coleman,C.S., Pickart,C.M., Rapoport,T.A. and Chau,V.
  TITLE     E2-25K mediates US11-triggered retro-translocation of MHC class I
            heavy chains in a permeabilized cell system
  JOURNAL   Proc Natl Acad Sci U S A 103 (31), 11589-11594 (2006)
   PUBMED   16868077
REFERENCE   8  (bases 1 to 6043)
  AUTHORS   Lim,J., Hao,T., Shaw,C., Patel,A.J., Szabo,G., Rual,J.F.,
            Fisk,C.J., Li,N., Smolyar,A., Hill,D.E., Barabasi,A.L., Vidal,M.
            and Zoghbi,H.Y.
  TITLE     A protein-protein interaction network for human inherited ataxias
            and disorders of Purkinje cell degeneration
  JOURNAL   Cell 125 (4), 801-814 (2006)
   PUBMED   16713569
REFERENCE   9  (bases 1 to 6043)
  AUTHORS   Metcalfe,S.M., Muthukumarana,P.A., Thompson,H.L., Haendel,M.A. and
            Lyons,G.E.
  TITLE     Leukaemia inhibitory factor (LIF) is functionally linked to
            axotrophin and both LIF and axotrophin are linked to regulatory
            immune tolerance
  JOURNAL   FEBS Lett 579 (3), 609-614 (2005)
   PUBMED   15670816
REFERENCE   10 (bases 1 to 6043)
  AUTHORS   Bartee,E., Mansouri,M., Hovey Nerenberg,B.T., Gouveia,K. and
            Fruh,K.
  TITLE     Downregulation of major histocompatibility complex class I by human
            ubiquitin ligases related to viral immune evasion proteins
  JOURNAL   J Virol 78 (3), 1109-1120 (2004)
   PUBMED   14722266
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK292836.1, AK316382.1, BC003404.1, AI863394.1 and AC009961.11.
            
            On Nov 22, 2018 this sequence version replaced NM_001282805.1.
            
            Summary: MARCH7 is a member of the MARCH family of membrane-bound
            E3 ubiquitin ligases (EC 6.3.2.19). MARCH proteins add ubiquitin
            (see MIM 191339) to target lysines in substrate proteins, thereby
            signaling their vesicular transport between membrane compartments
            (Bartee et al., 2004 [PubMed 14722266]).[supplied by OMIM, Mar
            2010].
            
            Transcript Variant: This variant (1) encodes the longest isoform
            (a). Variants 1, 2, and 5-13 all encode the same isoform (a).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK292836.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1968540
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000409175.6/ ENSP00000386830.1
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1709              AK292836.1         18-1726
            1710-1710           AK316382.1         1463-1463
            1711-2385           AK292836.1         1728-2402
            2386-3589           BC003404.1         706-1909
            3590-3599           AI863394.1         17-26               c
            3600-6043           AC009961.11        130629-133072
FEATURES             Location/Qualifiers
     source          1..6043
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="2"
                     /map="2q24.2"
     gene            1..6043
                     /gene="MARCHF7"
                     /gene_synonym="AXO; AXOT; MARCH-VII; MARCH7; RNF177"
                     /note="membrane associated ring-CH-type finger 7"
                     /db_xref="GeneID:64844"
                     /db_xref="HGNC:HGNC:17393"
                     /db_xref="MIM:613334"
     exon            1..101
                     /gene="MARCHF7"
                     /gene_synonym="AXO; AXOT; MARCH-VII; MARCH7; RNF177"
                     /inference="alignment:Splign:2.1.0"
     exon            102..143
                     /gene="MARCHF7"
                     /gene_synonym="AXO; AXOT; MARCH-VII; MARCH7; RNF177"
                     /inference="alignment:Splign:2.1.0"
     exon            144..229
                     /gene="MARCHF7"
                     /gene_synonym="AXO; AXOT; MARCH-VII; MARCH7; RNF177"
                     /inference="alignment:Splign:2.1.0"
     exon            230..396
                     /gene="MARCHF7"
                     /gene_synonym="AXO; AXOT; MARCH-VII; MARCH7; RNF177"
                     /inference="alignment:Splign:2.1.0"
     CDS             244..2358
                     /gene="MARCHF7"
                     /gene_synonym="AXO; AXOT; MARCH-VII; MARCH7; RNF177"
                     /EC_number="2.3.2.27"
                     /note="isoform a is encoded by transcript variant 1;
                     axotrophin; membrane-associated RING-CH protein VII; E3
                     ubiquitin-protein ligase MARCH7; RING finger protein 177;
                     membrane associated ring finger 7; membrane-associated
                     RING finger protein 7; membrane-associated ring finger
                     (C3HC4) 7, E3 ubiquitin protein ligase; RING-type E3
                     ubiquitin transferase MARCH7; RING-type E3 ubiquitin
                     transferase MARCHF7; E3 ubiquitin-protein ligase MARCHF7"
                     /codon_start=1
                     /product="E3 ubiquitin-protein ligase MARCHF7 isoform a"
                     /protein_id="NP_001269734.1"
                     /db_xref="CCDS:CCDS2210.1"
                     /db_xref="GeneID:64844"
                     /db_xref="HGNC:HGNC:17393"
                     /db_xref="MIM:613334"
                     /translation="MESKPSRIPRRISVQPSSSLSARMMSGSRGSSLNDTYHSRDSSF
                     RLDSEYQSTSASASASPFQSAWYSESEITQGARSRSQNQQRDHDSKRPKLSCTNCTTS
                     AGRNVGNGLNTLSDSSWRHSQVPRSSSMVLGSFGTDLMRERRDLERRTDSSISNLMDY
                     SHRSGDFTTSSYVQDRVPSYSQGARPKENSMSTLQLNTSSTNHQLPSEHQTILSSRDS
                     RNSLRSNFSSRESESSRSNTQPGFSYSSSRDEAPIISNSERVVSSQRPFQESSDNEGR
                     RTTRRLLSRIASSMSSTFFSRRSSQDSLNTRSLNSENSYVSPRILTASQSRSNVPSAS
                     EVPDNRASEASQGFRFLRRRWGLSSLSHNHSSESDSENFNQESEGRNTGPWLSSSLRN
                     RCTPLFSRRRREGRDESSRIPTSDTSSRSHIFRRESNEVVHLEAQNDPLGAAANRPQA
                     SAASSSATTGGSTSDSAQGGRNTGISGILPGSLFRFAVPPALGSNLTDNVMITVDIIP
                     SGWNSADGKSDKTKSAPSRDPERLQKIKESLLLEDSEEEEGDLCRICQMAAASSSNLL
                     IEPCKCTGSLQYVHQDCMKKWLQAKINSGSSLEAVTTCELCKEKLELNLEDFDIHELH
                     RAHANEQAEYEFISSGLYLVVLLHLCEQSFSDMMGNTNEPSTRVRFINLARTLQAHME
                     DLETSEDDSEEDGDHNRTFDIA"
     exon            397..589
                     /gene="MARCHF7"
                     /gene_synonym="AXO; AXOT; MARCH-VII; MARCH7; RNF177"
                     /inference="alignment:Splign:2.1.0"
     exon            590..757
                     /gene="MARCHF7"
                     /gene_synonym="AXO; AXOT; MARCH-VII; MARCH7; RNF177"
                     /inference="alignment:Splign:2.1.0"
     exon            758..1856
                     /gene="MARCHF7"
                     /gene_synonym="AXO; AXOT; MARCH-VII; MARCH7; RNF177"
                     /inference="alignment:Splign:2.1.0"
     exon            1857..2026
                     /gene="MARCHF7"
                     /gene_synonym="AXO; AXOT; MARCH-VII; MARCH7; RNF177"
                     /inference="alignment:Splign:2.1.0"
     exon            2027..2136
                     /gene="MARCHF7"
                     /gene_synonym="AXO; AXOT; MARCH-VII; MARCH7; RNF177"
                     /inference="alignment:Splign:2.1.0"
     exon            2137..2250
                     /gene="MARCHF7"
                     /gene_synonym="AXO; AXOT; MARCH-VII; MARCH7; RNF177"
                     /inference="alignment:Splign:2.1.0"
     exon            2251..2299
                     /gene="MARCHF7"
                     /gene_synonym="AXO; AXOT; MARCH-VII; MARCH7; RNF177"
                     /inference="alignment:Splign:2.1.0"
     exon            2300..6043
                     /gene="MARCHF7"
                     /gene_synonym="AXO; AXOT; MARCH-VII; MARCH7; RNF177"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3569..3574
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MARCHF7"
                     /gene_synonym="AXO; AXOT; MARCH-VII; MARCH7; RNF177"
                     /note="hexamer: AATAAA"
     polyA_site      3589
                     /gene="MARCHF7"
                     /gene_synonym="AXO; AXOT; MARCH-VII; MARCH7; RNF177"
                     /note="major polyA site"
     regulatory      6012..6017
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MARCHF7"
                     /gene_synonym="AXO; AXOT; MARCH-VII; MARCH7; RNF177"
                     /note="hexamer: AATAAA"
     polyA_site      6043
                     /gene="MARCHF7"
                     /gene_synonym="AXO; AXOT; MARCH-VII; MARCH7; RNF177"
ORIGIN      
        1 ggttctgcgc cggatccggg agaggggcgg gcgccattgt gcttcgctgc cgactgcatt
       61 tcctcagtca cgggcctaga actccaagga gaaaggcggc ggaattcatg atttgttcct
      121 gtagctgaaa catacattga atgctttctg ccctggcatg aacttacatg gtcagagctg
      181 gtttttcctg ccctcaaata tatttctctg actctaccgg ttagactaga aaaatcttta
      241 agaatggagt ctaaaccttc aaggattcca agaagaattt ctgttcaacc ttccagctcc
      301 ttaagtgcta ggatgatgtc tggaagcaga ggaagtagtt taaatgatac ctatcactca
      361 agagactctt catttagatt ggattctgaa tatcagtcta catcagcatc agcatctgcg
      421 tcaccatttc aatctgcatg gtatagtgaa tctgagataa ctcagggagc acgctcaaga
      481 tcgcagaacc agcaacggga tcatgattca aaaagaccta aactttcctg tacaaactgt
      541 actacctcag ctgggagaaa tgttggaaat ggtttaaaca cattatcaga ttcatcttgg
      601 aggcatagtc aagttcctag atcttcatca atggtacttg gatcatttgg aacagactta
      661 atgagagaga ggagagattt ggagagaaga acagattcct ctattagtaa tcttatggat
      721 tatagtcacc gaagtggtga tttcacaact tcatcatatg ttcaagacag agttccttca
      781 tattcacaag gagcaagacc aaaagaaaac tcaatgagca ctttacagtt gaatacatca
      841 tccacaaacc accaattgcc ttctgaacat cagaccatac taagttctag ggactccaga
      901 aattctttaa gatcaaattt ttcttcaaga gaatcagaat cttcccgaag caatacgcag
      961 cctggatttt cttacagttc aagtagagat gaagccccaa tcataagcaa ttcagaaagg
     1021 gttgtttcat ctcaaagacc atttcaagaa tcttctgaca atgaaggtag gcggacaacg
     1081 aggagattgc tgtcacgcat agcttctagc atgtcatcta cttttttttc acgaagatct
     1141 agtcaggatt ccttgaatac aagatcattg aattctgaaa attcttacgt ttctccaaga
     1201 atcttgacag cttcacagtc ccgtagtaat gtaccatcag cttctgaagt tcccgataat
     1261 agggcatctg aagcttctca gggatttcga tttcttaggc gaagatgggg tttgtcatct
     1321 cttagccaca atcatagctc tgagtcagat tcagaaaatt ttaaccaaga atctgaaggt
     1381 agaaatacag gaccatggtt atcttcctca cttagaaata gatgcacacc tttgttctct
     1441 agaaggaggc gagagggaag agatgaatct tcaaggatac ctacctctga tacatcatct
     1501 agatctcata tttttagaag agaatcaaat gaagtggttc accttgaagc acagaatgat
     1561 cctcttggag ctgctgccaa cagaccacaa gcatctgcag catcaagcag tgccacaaca
     1621 ggtggctcta catcagattc ggctcaaggt ggaagaaata caggaatatc agggattctt
     1681 cctggttcct tattccggtt tgcagtccct ccagcacttg ggagtaattt gaccgacaat
     1741 gtcatgatca cagtagatat tattccttca ggttggaatt cagctgatgg taaaagtgat
     1801 aaaactaaaa gtgcgccttc aagagatcca gaaagattgc agaaaataaa agagagcctc
     1861 cttttagagg actcagaaga agaagaaggt gacttatgta gaatttgtca aatggcagct
     1921 gcatcatcat ctaatttgct gatagagcca tgcaagtgca caggaagttt gcagtatgtc
     1981 caccaagact gtatgaaaaa gtggttacag gccaaaatta actctggttc ttcattagaa
     2041 gctgtaacca cctgtgaact atgtaaagag aagttggagc ttaacctgga ggattttgat
     2101 attcatgaac tacatagagc tcatgcaaat gaacaagctg agtatgagtt tatcagctct
     2161 ggtctctacc tagtggtgtt attgcacttg tgcgaacaaa gcttttctga tatgatggga
     2221 aatacaaatg aaccaagcac acgtgtccga tttattaacc ttgcaagaac tcttcaggca
     2281 catatggaag atctcgaaac ttcagaggat gattccgaag aagacggaga ccataacagg
     2341 acatttgata ttgcctaact tcatataaga cagatggatg atctgtgaac ataagtgttt
     2401 attaaaaatg gcaattaaat ataaattact tttgtggggg aatgcctaat aaatacattg
     2461 actatatata aaatgaatat atacatacac atgtatgcct gtatatatat attcattctc
     2521 cagtgttgct gaattaaaat tctgctggac tttttaacat agcaaatccg atgtttataa
     2581 actggtaatc aaaaaggttt tttcttttag gtgagtggga aagtattacc cttgttttaa
     2641 atatctaagc aatgcctatc aacccttttt tgtgttatga ttactgtagt catatttatg
     2701 aaaaaaggtt tgtgttttac tcttgctagt gagaaaagtg ggacaaaata tacttttgaa
     2761 ataaaatgct atatggcacc taattatttt ttcttttaaa atgccttaag ttgcagtctc
     2821 attttgataa tcatttgctt ccagtgttta aaaattaaaa aaagaatggg gagaaggtta
     2881 tgagaagagc attattaagt ttccaaattt aatttgaatt ccaaattcac ctagcaataa
     2941 aatctaattt ttaaaaagta tataaatata aaatgtataa atgatggata gatttttgta
     3001 ttgatttgca aaatgcagat tatatttgat aggctatagt atgtagatat tccttttagg
     3061 aatattacag ctgtaaatta tatgagactt gccagtcaaa tgctatttgg tttaaaaaaa
     3121 ttattgcaat ctcaagttaa tggaatattt ttaaatccca cattcagagt ttaaaacact
     3181 ggttttcaat gtgtttttta gtgttgtcac ttgtttatag ataaatatat aaataacctg
     3241 tttggatcct ggtccttttt aactgttcct tggtaattct gagcatttat ttgatgactt
     3301 aatatttttc actacctttg gagaacagat gaacattatt caccatgaat ggatctatac
     3361 tgtgtggtca tgagttgtgt atacttccat aacactgtat ttttcttctg tcagtaccct
     3421 taggatacac tttaaaacac cttaaggtct gatgttatgg caacaaacta ctttttcaaa
     3481 cctaaatagg aaccatgtaa tttctcaaaa gtgattgaac agtttgccca cacttagttt
     3541 gttggtctta tgtaaaacat tggctcaaaa taaagtacac actgatttat tttactgttt
     3601 gaaatgtttc cttttaaact ggtgctcaaa gaagacatca gacttcataa ccaagaattt
     3661 tattacaatt tcaaatctca ttttaagcaa taatataaat gttctaaaac atttgctcac
     3721 ctcttgccag ttacctttgc attgtctgtc ttaaagcaaa aatataaaat ttaagggtgg
     3781 tagttttaat atattctgtg ggtcctaagg gatgctttga atttgaagta tatatacttt
     3841 taataaaatt cagtatgaca gttgtcttct gcttaaccca taaaacttta tttaaaaaat
     3901 ttaactagat ggtacaaaac attcattggt atgcacataa ttctgtatag ttttgttatt
     3961 aaatgctaaa tgacagtgat aacaatggct taagctttac agtattcttg tagttcccta
     4021 gtaccactta gtatttaaat attgtcatat tagcttcaga ttatcatgta aatgacggta
     4081 gaggaataag agaaaaggct ttcattggtt atcagtttta aattctgaac aaaagagacc
     4141 atacactgct cactacaaga atgcaatttt ctaagaaaat gtagtttaaa atgttattct
     4201 gaatatctgc caaagaatta tattgttatt actagctaga accaagatgt tgaagaaagc
     4261 agtttctaca ttctggatgt agtaaacaag cctgcctgtg taatccaatt atctcttcac
     4321 taatgagtag ataaggtgcc tgttatagat gttctggtat ataaagaaaa aatgtaggcc
     4381 aagtgtggtg gctcacgcat gtaatcccag cactttggga gaccaaggtg ggtggatcac
     4441 ttgaggtcag gagtttgaga ccagcccagt caatatggcg aaaccccgtc tctaccaaaa
     4501 atacaaaaag ttagctgggt gtggtgacag atgtctgtgg tcccagctac taaggaggct
     4561 gaggcaggag aattgcttga aaacctgcaa ggcggagatt ggagagagcc aagatcgagc
     4621 cactgcactg tagcctgggc agcacagtga gactccatca tatatatata tatagcactt
     4681 cattaccaga ggcctcttgg cctgttgaaa aatgaaacca aatattgaaa attaaatcag
     4741 agtttaaagc agctattttg gaagagctgc cattaggtag aaagtatcaa aatgtacaaa
     4801 agaaagttgt atgggagttg agtggaaggt aggatttaag agcagcactg cccaataaaa
     4861 cttcctataa tgaaaatgtt ctataatgta tgttgcccag tacagtagcc actaaccacc
     4921 tgtgatttga acacttgaca atgtggctaa tgtaattcga aaactggatt ttaaactaaa
     4981 tttacttaaa tagcctcatg tggctagtgg ctcattggac attgtagttg tagacgtttg
     5041 agactgttgg ttttaagttg gacttaatca ctttcctacc caaattctac cactccttta
     5101 agaactcctt tagaactctt ttagttcaca taatacgcca tatttttttt actgtgcctg
     5161 tagttcttca aggagtggta caatttgggt aggaaaacca ggcaggaatt ccagggtagt
     5221 gttcaatatt gacattagta atagtctatc aataataaaa tagacatctc aatcactata
     5281 caaaatctca gaaatgtaaa gctcttacag agcatgcttg tgcttgtgta acagctggtg
     5341 taatgcctgc attttcagta ccatgtagcc gcactgttaa tagttttcta tcacttttta
     5401 gttactcatg tctcattaat gatagtgcca ttaattgtga tgagtgtttt cgattcatgt
     5461 ggtcaataaa aagagactac acaagctgga actttgttgc cattagtcaa gctagtgaga
     5521 tagtatatct atctatctcc ccagaagaaa gtaagataat tgatggggtg tggattcaga
     5581 agagggatta cttttctttg agcctcagac ttctagacag tatacttcag tcagtaatgg
     5641 accacatata gaacagtgtt tccttagtag accatatttt tactgtacct tttctatatt
     5701 tagatacaca aatattgtgt tacaattgtc tgcagtattc agcacagtaa catgctgtgt
     5761 aggtttggga caaaataggc tctaccatct gggtttgtgt aaatacaagc tgattttcac
     5821 acaagattcc ctaactatgc atttcttaga acgtatcccc attgataagt gatacgtgac
     5881 taatttacgt gaaatttata cattctttat ctttcctgtt tttggtttat tgatggtgag
     5941 gaaaattact cgtttcagct ttttcatttt tttactcccc aaatgatttt cacctttttc
     6001 ttaaaatgta caataaatgc actgaaaact ttgatcactg tca
//
