LOCUS       NM_005885               9641 bp    mRNA    linear   PRI 04-APR-2024
DEFINITION  Homo sapiens membrane associated ring-CH-type finger 6 (MARCHF6),
            transcript variant 1, mRNA.
ACCESSION   NM_005885
VERSION     NM_005885.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 9641)
  AUTHORS   Liu,Y., Xu,S., Huang,Y., Liu,S., Xu,Z., Wei,M. and Liu,J.
  TITLE     MARCH6 promotes Papillary Thyroid Cancer development by
            destabilizing DHX9
  JOURNAL   Int J Biol Sci 17 (13), 3401-3412 (2021)
   PUBMED   34512155
  REMARK    GeneRIF: MARCH6 promotes Papillary Thyroid Cancer development by
            destabilizing DHX9.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9641)
  AUTHORS   Sun,J., Dong,Z., Chang,Z., Liu,H., Jiang,Q., Zhang,D., Lu,S.,
            Jia,X., Wu,D., Ge,A., Zhao,P., Wang,J. and Lu,Y.
  TITLE     MARCH6 promotes hepatocellular carcinoma development through
            up-regulation of ATF2
  JOURNAL   BMC Cancer 21 (1), 827 (2021)
   PUBMED   34273954
  REMARK    GeneRIF: MARCH6 promotes hepatocellular carcinoma development
            through up-regulation of ATF2.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 9641)
  AUTHORS   Scott,N.A., Sharpe,L.J. and Brown,A.J.
  TITLE     The E3 ubiquitin ligase MARCHF6 as a metabolic integrator in
            cholesterol synthesis and beyond
  JOURNAL   Biochim Biophys Acta Mol Cell Biol Lipids 1866 (1), 158837 (2021)
   PUBMED   33049405
  REMARK    GeneRIF: The E3 ubiquitin ligase MARCHF6 as a metabolic integrator
            in cholesterol synthesis and beyond.
            Review article
REFERENCE   4  (bases 1 to 9641)
  AUTHORS   Tan,J.M.E., van der Stoel,M.M., van den Berg,M., van Loon,N.M.,
            Moeton,M., Scholl,E., van der Wel,N.N., Kovacevic,I., Hordijk,P.L.,
            Loregger,A., Huveneers,S. and Zelcer,N.
  TITLE     The MARCH6-SQLE Axis Controls Endothelial Cholesterol Homeostasis
            and Angiogenic Sprouting
  JOURNAL   Cell Rep 32 (5), 107944 (2020)
   PUBMED   32755570
  REMARK    GeneRIF: The MARCH6-SQLE Axis Controls Endothelial Cholesterol
            Homeostasis and Angiogenic Sprouting.
REFERENCE   5  (bases 1 to 9641)
  AUTHORS   Wang,L., Dong,H., Soroka,C.J., Wei,N., Boyer,J.L. and
            Hochstrasser,M.
  TITLE     Degradation of the bile salt export pump at endoplasmic reticulum
            in progressive familial intrahepatic cholestasis type II
  JOURNAL   Hepatology 48 (5), 1558-1569 (2008)
   PUBMED   18798335
REFERENCE   6  (bases 1 to 9641)
  AUTHORS   Kreft,S.G., Wang,L. and Hochstrasser,M.
  TITLE     Membrane topology of the yeast endoplasmic reticulum-localized
            ubiquitin ligase Doa10 and comparison with its human ortholog TEB4
            (MARCH-VI)
  JOURNAL   J Biol Chem 281 (8), 4646-4653 (2006)
   PUBMED   16373356
  REMARK    GeneRIF: analysis of yeast endoplasmic reticulum-localized
            ubiquitin ligase Doa10 and comparison with its human ortholog TEB4
REFERENCE   7  (bases 1 to 9641)
  AUTHORS   Hassink,G., Kikkert,M., van Voorden,S., Lee,S.J., Spaapen,R., van
            Laar,T., Coleman,C.S., Bartee,E., Fruh,K., Chau,V. and Wiertz,E.
  TITLE     TEB4 is a C4HC3 RING finger-containing ubiquitin ligase of the
            endoplasmic reticulum
  JOURNAL   Biochem J 388 (Pt 2), 647-655 (2005)
   PUBMED   15673284
REFERENCE   8  (bases 1 to 9641)
  AUTHORS   Bartee,E., Mansouri,M., Hovey Nerenberg,B.T., Gouveia,K. and
            Fruh,K.
  TITLE     Downregulation of major histocompatibility complex class I by human
            ubiquitin ligases related to viral immune evasion proteins
  JOURNAL   J Virol 78 (3), 1109-1120 (2004)
   PUBMED   14722266
REFERENCE   9  (bases 1 to 9641)
  AUTHORS   Swanson,R., Locher,M. and Hochstrasser,M.
  TITLE     A conserved ubiquitin ligase of the nuclear envelope/endoplasmic
            reticulum that functions in both ER-associated and Matalpha2
            repressor degradation
  JOURNAL   Genes Dev 15 (20), 2660-2674 (2001)
   PUBMED   11641273
REFERENCE   10 (bases 1 to 9641)
  AUTHORS   Simmons,A.D., Overhauser,J. and Lovett,M.
  TITLE     Isolation of cDNAs from the Cri-du-chat critical region by direct
            screening of a chromosome 5-specific cDNA library
  JOURNAL   Genome Res 7 (2), 118-127 (1997)
   PUBMED   9049630
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DB072938.1, AB011169.1,
            AC092336.2 and BX116291.1.
            
            On Nov 22, 2018 this sequence version replaced NM_005885.3.
            
            Summary: This gene encodes a member of a family of
            membrane-associated E3 ubiquitin ligases containing RING-CH-type
            zinc finger motifs. Ubiquitination of type II deiodinase by the
            encoded protein is an important regulatory step in thyroid hormone
            signalling. Alternatively spliced transcript variants encoding
            multiple isoforms have been observed for this gene. [provided by
            RefSeq, Jul 2012].
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AB011169.1, BC136462.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000274140.10/ ENSP00000274140.4
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-21                DB072938.1         57-77
            22-4632             AB011169.1         1-4611
            4633-9507           AC092336.2         69111-73985         c
            9508-9641           BX116291.1         14-147              c
FEATURES             Location/Qualifiers
     source          1..9641
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="5"
                     /map="5p15.2"
     gene            1..9641
                     /gene="MARCHF6"
                     /gene_synonym="DOA10; FAME3; FCMTE3; MARCH-VI; MARCH6;
                     RNF176; TEB4"
                     /note="membrane associated ring-CH-type finger 6"
                     /db_xref="GeneID:10299"
                     /db_xref="HGNC:HGNC:30550"
                     /db_xref="MIM:613297"
     exon            1..223
                     /gene="MARCHF6"
                     /gene_synonym="DOA10; FAME3; FCMTE3; MARCH-VI; MARCH6;
                     RNF176; TEB4"
                     /inference="alignment:Splign:2.1.0"
     CDS             205..2937
                     /gene="MARCHF6"
                     /gene_synonym="DOA10; FAME3; FCMTE3; MARCH-VI; MARCH6;
                     RNF176; TEB4"
                     /EC_number="2.3.2.27"
                     /note="isoform 1 is encoded by transcript variant 1;
                     membrane-associated RING-CH protein VI; E3
                     ubiquitin-protein ligase MARCH6; RING finger protein 176;
                     membrane-associated RING finger protein 6; doa10 homolog;
                     membrane-associated RING-CH finger protein 6;
                     membrane-associated ring finger (C3HC4) 6, E3 ubiquitin
                     protein ligase; membrane associated ring finger 6;
                     RING-type E3 ubiquitin transferase MARCH6; RING-type E3
                     ubiquitin transferase MARCHF6; E3 ubiquitin-protein ligase
                     MARCHF6"
                     /codon_start=1
                     /product="E3 ubiquitin-protein ligase MARCHF6 isoform 1"
                     /protein_id="NP_005876.2"
                     /db_xref="CCDS:CCDS34135.1"
                     /db_xref="GeneID:10299"
                     /db_xref="HGNC:HGNC:30550"
                     /db_xref="MIM:613297"
                     /translation="MDTAEEDICRVCRSEGTPEKPLYHPCVCTGSIKFIHQECLVQWL
                     KHSRKEYCELCKHRFAFTPIYSPDMPSRLPIQDIFAGLVTSIGTAIRYWFHYTLVAFA
                     WLGVVPLTACRIYKCLFTGSVSSLLTLPLDMLSTENLLADCLQGCFVVTCTLCAFISL
                     VWLREQIVHGGAPIWLEHAAPPFNAAGHHQNEAPAGGNGAENVAADQPANPPAENAVV
                     GENPDAQDDQAEEEEEDNEEEDDAGVEDAADANNGAQDDMNWNALEWDRAAEELTWER
                     MLGLDGSLVFLEHVFWVVSLNTLFILVFAFCPYHIGHFSLVGLGFEEHVQASHFEGLI
                     TTIVGYILLAITLIICHGLATLVKFHRSRRLLGVCYIVVKVSLLVVVEIGVFPLICGW
                     WLDICSLEMFDATLKDRELSFQSAPGTTMFLHWLVGMVYVFYFASFILLLREVLRPGV
                     LWFLRNLNDPDFNPVQEMIHLPIYRHLRRFILSVIVFGSIVLLMLWLPIRIIKSVLPN
                     FLPYNVMLYSDAPVSELSLELLLLQVVLPALLEQGHTRQWLKGLVRAWTVTAGYLLDL
                     HSYLLGDQEENENSANQQVNNNQHARNNNAIPVVGEGLHAAHQAILQQGGPVGFQPYR
                     RPLNFPLRIFLLIVFMCITLLIASLICLTLPVFAGRWLMSFWTGTAKIHELYTAACGL
                     YVCWLTIRAVTVMVAWMPQGRRVIFQKVKEWSLMIMKTLIVAVLLAGVVPLLLGLLFE
                     LVIVAPLRVPLDQTPLFYPWQDWALGVLHAKIIAAITLMGPQWWLKTVIEQVYANGIR
                     NIDLHYIVRKLAAPVISVLLLSLCVPYVIASGVVPLLGVTAEMQNLVHRRIYPFLLMV
                     VVLMAILSFQVRQFKRLYEHIKNDKYLVGQRLVNYERKSGKQGSSPPPPQSSQE"
     exon            224..320
                     /gene="MARCHF6"
                     /gene_synonym="DOA10; FAME3; FCMTE3; MARCH-VI; MARCH6;
                     RNF176; TEB4"
                     /inference="alignment:Splign:2.1.0"
     exon            321..394
                     /gene="MARCHF6"
                     /gene_synonym="DOA10; FAME3; FCMTE3; MARCH-VI; MARCH6;
                     RNF176; TEB4"
                     /inference="alignment:Splign:2.1.0"
     exon            395..538
                     /gene="MARCHF6"
                     /gene_synonym="DOA10; FAME3; FCMTE3; MARCH-VI; MARCH6;
                     RNF176; TEB4"
                     /inference="alignment:Splign:2.1.0"
     exon            539..611
                     /gene="MARCHF6"
                     /gene_synonym="DOA10; FAME3; FCMTE3; MARCH-VI; MARCH6;
                     RNF176; TEB4"
                     /inference="alignment:Splign:2.1.0"
     exon            612..780
                     /gene="MARCHF6"
                     /gene_synonym="DOA10; FAME3; FCMTE3; MARCH-VI; MARCH6;
                     RNF176; TEB4"
                     /inference="alignment:Splign:2.1.0"
     exon            781..970
                     /gene="MARCHF6"
                     /gene_synonym="DOA10; FAME3; FCMTE3; MARCH-VI; MARCH6;
                     RNF176; TEB4"
                     /inference="alignment:Splign:2.1.0"
     exon            971..1032
                     /gene="MARCHF6"
                     /gene_synonym="DOA10; FAME3; FCMTE3; MARCH-VI; MARCH6;
                     RNF176; TEB4"
                     /inference="alignment:Splign:2.1.0"
     exon            1033..1065
                     /gene="MARCHF6"
                     /gene_synonym="DOA10; FAME3; FCMTE3; MARCH-VI; MARCH6;
                     RNF176; TEB4"
                     /inference="alignment:Splign:2.1.0"
     exon            1066..1117
                     /gene="MARCHF6"
                     /gene_synonym="DOA10; FAME3; FCMTE3; MARCH-VI; MARCH6;
                     RNF176; TEB4"
                     /inference="alignment:Splign:2.1.0"
     exon            1118..1176
                     /gene="MARCHF6"
                     /gene_synonym="DOA10; FAME3; FCMTE3; MARCH-VI; MARCH6;
                     RNF176; TEB4"
                     /inference="alignment:Splign:2.1.0"
     exon            1177..1257
                     /gene="MARCHF6"
                     /gene_synonym="DOA10; FAME3; FCMTE3; MARCH-VI; MARCH6;
                     RNF176; TEB4"
                     /inference="alignment:Splign:2.1.0"
     exon            1258..1326
                     /gene="MARCHF6"
                     /gene_synonym="DOA10; FAME3; FCMTE3; MARCH-VI; MARCH6;
                     RNF176; TEB4"
                     /inference="alignment:Splign:2.1.0"
     exon            1327..1401
                     /gene="MARCHF6"
                     /gene_synonym="DOA10; FAME3; FCMTE3; MARCH-VI; MARCH6;
                     RNF176; TEB4"
                     /inference="alignment:Splign:2.1.0"
     exon            1402..1536
                     /gene="MARCHF6"
                     /gene_synonym="DOA10; FAME3; FCMTE3; MARCH-VI; MARCH6;
                     RNF176; TEB4"
                     /inference="alignment:Splign:2.1.0"
     exon            1537..1656
                     /gene="MARCHF6"
                     /gene_synonym="DOA10; FAME3; FCMTE3; MARCH-VI; MARCH6;
                     RNF176; TEB4"
                     /inference="alignment:Splign:2.1.0"
     exon            1657..1757
                     /gene="MARCHF6"
                     /gene_synonym="DOA10; FAME3; FCMTE3; MARCH-VI; MARCH6;
                     RNF176; TEB4"
                     /inference="alignment:Splign:2.1.0"
     exon            1758..1895
                     /gene="MARCHF6"
                     /gene_synonym="DOA10; FAME3; FCMTE3; MARCH-VI; MARCH6;
                     RNF176; TEB4"
                     /inference="alignment:Splign:2.1.0"
     exon            1896..2100
                     /gene="MARCHF6"
                     /gene_synonym="DOA10; FAME3; FCMTE3; MARCH-VI; MARCH6;
                     RNF176; TEB4"
                     /inference="alignment:Splign:2.1.0"
     exon            2101..2170
                     /gene="MARCHF6"
                     /gene_synonym="DOA10; FAME3; FCMTE3; MARCH-VI; MARCH6;
                     RNF176; TEB4"
                     /inference="alignment:Splign:2.1.0"
     exon            2171..2352
                     /gene="MARCHF6"
                     /gene_synonym="DOA10; FAME3; FCMTE3; MARCH-VI; MARCH6;
                     RNF176; TEB4"
                     /inference="alignment:Splign:2.1.0"
     exon            2353..2487
                     /gene="MARCHF6"
                     /gene_synonym="DOA10; FAME3; FCMTE3; MARCH-VI; MARCH6;
                     RNF176; TEB4"
                     /inference="alignment:Splign:2.1.0"
     exon            2488..2577
                     /gene="MARCHF6"
                     /gene_synonym="DOA10; FAME3; FCMTE3; MARCH-VI; MARCH6;
                     RNF176; TEB4"
                     /inference="alignment:Splign:2.1.0"
     exon            2578..2710
                     /gene="MARCHF6"
                     /gene_synonym="DOA10; FAME3; FCMTE3; MARCH-VI; MARCH6;
                     RNF176; TEB4"
                     /inference="alignment:Splign:2.1.0"
     exon            2711..2846
                     /gene="MARCHF6"
                     /gene_synonym="DOA10; FAME3; FCMTE3; MARCH-VI; MARCH6;
                     RNF176; TEB4"
                     /inference="alignment:Splign:2.1.0"
     exon            2847..9641
                     /gene="MARCHF6"
                     /gene_synonym="DOA10; FAME3; FCMTE3; MARCH-VI; MARCH6;
                     RNF176; TEB4"
                     /inference="alignment:Splign:2.1.0"
     regulatory      6274..6279
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MARCHF6"
                     /gene_synonym="DOA10; FAME3; FCMTE3; MARCH-VI; MARCH6;
                     RNF176; TEB4"
                     /note="hexamer: ATTAAA"
     polyA_site      6293
                     /gene="MARCHF6"
                     /gene_synonym="DOA10; FAME3; FCMTE3; MARCH-VI; MARCH6;
                     RNF176; TEB4"
                     /note="major polyA site"
     regulatory      7081..7086
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MARCHF6"
                     /gene_synonym="DOA10; FAME3; FCMTE3; MARCH-VI; MARCH6;
                     RNF176; TEB4"
                     /note="hexamer: AATAAA"
     polyA_site      7102
                     /gene="MARCHF6"
                     /gene_synonym="DOA10; FAME3; FCMTE3; MARCH-VI; MARCH6;
                     RNF176; TEB4"
                     /note="major polyA site"
     regulatory      9478..9483
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MARCHF6"
                     /gene_synonym="DOA10; FAME3; FCMTE3; MARCH-VI; MARCH6;
                     RNF176; TEB4"
                     /note="hexamer: AATAAA"
     polyA_site      9503
                     /gene="MARCHF6"
                     /gene_synonym="DOA10; FAME3; FCMTE3; MARCH-VI; MARCH6;
                     RNF176; TEB4"
     regulatory      9620..9625
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MARCHF6"
                     /gene_synonym="DOA10; FAME3; FCMTE3; MARCH-VI; MARCH6;
                     RNF176; TEB4"
                     /note="hexamer: AATAAA"
     polyA_site      9641
                     /gene="MARCHF6"
                     /gene_synonym="DOA10; FAME3; FCMTE3; MARCH-VI; MARCH6;
                     RNF176; TEB4"
ORIGIN      
        1 gttcccgccc ctccccctcc cgtgtcgctc gctttctgtc agcctctctc cctctccctc
       61 tcccctctcc ttcctctcgc ttcctctctc gcacctgagc gtacgcacct gcccgggccc
      121 ggctccctcc tcctctcccc tccctctttc cccgcccggc cgcgggagcc tcgtggctgc
      181 gtcaccgccg cccccccaga caagatggac accgcggagg aagacatatg tagagtgtgt
      241 cggtcagaag gaacacctga gaaaccgctt tatcatcctt gtgtatgtac tggcagtatt
      301 aagtttatcc atcaagaatg cttagttcaa tggctgaaac acagtcgaaa agaatactgt
      361 gaattatgca agcacagatt tgcttttaca ccaatttatt ctccagatat gccttcacgg
      421 cttccaattc aagacatatt tgctggactg gttacaagta ttggcactgc aatacgatat
      481 tggtttcatt atacacttgt ggcctttgca tggttgggag ttgttcctct tacagcatgc
      541 cgcatctaca agtgcttgtt tactggctcc gtgagctcac tactgacgct gccattagat
      601 atgctgtcaa cggaaaattt gttggcagat tgtttgcagg gttgttttgt ggtgacgtgc
      661 acactgtgtg cattcatcag cctggtgtgg ttgagagagc agatagtcca tgggggagca
      721 ccaatttggt tggagcatgc tgccccaccg ttcaatgctg cggggcatca ccaaaatgag
      781 gctccagcag gaggaaatgg tgcagaaaat gttgctgctg atcagcctgc taacccacca
      841 gctgagaacg cagtggtggg ggaaaaccct gatgcccagg atgaccaggc agaagaggag
      901 gaggaggaca atgaggagga agatgacgct ggtgtggagg atgcggcaga tgctaataac
      961 ggagcccagg atgacatgaa ttggaatgct ttagaatggg accgagctgc tgaagagctt
     1021 acatgggaaa gaatgctagg acttgatgga tcactagttt ttctggaaca tgtcttctgg
     1081 gtggtatctt taaatacact gttcattctt gtttttgcat tttgccctta ccatattggt
     1141 catttctccc ttgttggttt gggatttgaa gaacacgtcc aagcatctca ttttgaaggc
     1201 ctaatcacaa ccatagttgg gtatatactt ttagcaataa cactgataat ttgtcatggc
     1261 ttggcaactc ttgtgaaatt tcatagatct cgtcgcttac tgggagtctg ctatattgtt
     1321 gttaaggtct ctttgttagt ggtggtagaa attggagtat tccctctcat ttgtggttgg
     1381 tggctggata tctgttcctt ggaaatgttt gatgctactc tgaaagatcg agaactgagc
     1441 tttcagtcgg ctccaggtac taccatgttt ctgcattggc tagtgggaat ggtatatgtc
     1501 ttctactttg cctccttcat tctactactg agagaggtac ttcgacctgg tgtcctgtgg
     1561 tttctaagga atttgaatga tccagatttc aatccagtac aggaaatgat ccatttgcca
     1621 atatataggc atctccgaag atttattttg tcagtgattg tctttggctc cattgtcctc
     1681 ctgatgcttt ggcttcctat acgtataatt aagagtgtgc tgcctaattt tcttccatac
     1741 aatgtcatgc tctacagtga tgctccagtg agtgaactgt ccctcgagct gcttctgctt
     1801 caggttgtct tgccagcatt actcgaacag ggacacacga ggcagtggct gaaggggctg
     1861 gtgcgagcgt ggactgtgac cgccggatac ttgctggatc ttcattctta tttattggga
     1921 gaccaggaag aaaatgaaaa cagtgcaaat caacaagtta acaataatca gcatgctcga
     1981 aataacaacg ctattcctgt ggtgggagaa ggccttcatg cagcccacca agccatactc
     2041 cagcagggag ggcctgttgg ctttcagcct taccgccgac ctttaaattt tccactcagg
     2101 atatttctgt tgattgtctt catgtgtata acattactga ttgccagcct catctgcctt
     2161 actttaccag tatttgctgg ccgttggtta atgtcgtttt ggacggggac tgccaaaatc
     2221 catgagctct acacagctgc ttgtggtctc tatgtttgct ggctaaccat aagggctgtg
     2281 acggtgatgg tggcatggat gcctcaggga cgcagagtga tcttccagaa ggttaaagag
     2341 tggtctctca tgatcatgaa gactttgata gttgcggtgc tgttggctgg agttgtccct
     2401 ctccttctgg ggctcctgtt tgagctggtc attgtggctc ccctgagggt tcccttggat
     2461 cagactcctc ttttttatcc atggcaggac tgggcacttg gagtcctgca tgccaaaatc
     2521 attgcagcta taacattgat gggtcctcag tggtggttga aaactgtaat tgaacaggtt
     2581 tacgcaaatg gcatccggaa cattgacctt cactatattg ttcgtaaact ggcagctccc
     2641 gtgatctctg tgctgttgct ttccctgtgt gtaccttatg tcatagcttc tggtgttgtt
     2701 cctttactag gtgttactgc ggaaatgcaa aacttagtcc atcggcggat ttatccattt
     2761 ttactgatgg tcgtggtatt gatggcaatt ttgtccttcc aagtccgcca gtttaagcgc
     2821 ctttatgaac atattaaaaa tgacaagtac cttgtgggtc aacgactcgt gaactacgaa
     2881 cggaaatctg gcaaacaagg ctcatctcca ccacctccac agtcatccca agaataaagt
     2941 agttgtctca acaacttgac cttccccttt acatgtcctt ttttgtggac ttctctcttt
     3001 ggagattttt cccagtgatc tctcagcgtt gtttttaagt taaatgtatt tgacttgtgt
     3061 tctcagcatt cagagagcag cggtgtaaga ttctgctgtt ctccctggat cttctgacat
     3121 tactgctgtc tgagatttgt atatgtgtaa atacaagttc cttgataccc taaaaccttg
     3181 gattaaacag aatgtgcatt gtacatcttt aaacaaaatg tatattaatt tattaaatct
     3241 agttgtcact ttattttgga cctgctgtga tctcgacagg aaacgtgcca cagagcagta
     3301 gtgcgcaggc aagacttttc agtgacgcct tgtggaacgc agttcatgat gtcctagcag
     3361 ctctcactaa gggaactgta cattctttct ttcttggcta ttcagacctt accaagaacg
     3421 ttaaaggaaa caagtagaaa tcagcagtgg agtgtctgtg gtaagaaaac atgaacttta
     3481 tgcttcactg ttagttgttt gtggaagtta ttttgtataa caccaaagct gttgtacatt
     3541 tcctactgcc tgattttttt catgtgtctg tgtttgtaat attgtatagt atcttgtgct
     3601 aggtgaggaa attattttta attttgataa tttaatattc ctagtgtgat cagcattggg
     3661 agttgggttt cagtggggca tgtctatact tagagaaaaa aagtccaaat gaagattttc
     3721 atgagtcagc ccccccgccc gcccccaccc cacacccaca tcctctcttt tccacacaca
     3781 actatctgtt tattttttgt agcagtggcc gaaagtcctg caaggtcata aatctttcag
     3841 agtgacatca ccaactgtac tgcatcttac tggatttagg acttctgaga tgcttgtgaa
     3901 gtatagatgt ggttgtggtc ttagattgac agcattagag aagactggtt agaacatctg
     3961 gtctcgctgg ttagtgcctc gttggctgag gactaggtgt gcatttctcc tagcttttca
     4021 tcaggaaatc ccaaagtttc caaagctttt tgtttacaga ataaaacttc aaataaaacc
     4081 aattcattat ttgtccagaa ggaagcttgg ctgagctggc cttttaacat aggaatgtat
     4141 ttcgttggaa acattctgaa aaatctcaga gaactgaacc cttacaaact ttgttttccc
     4201 tcataaccaa agcttcaggt tagaagttta gaaaaataga atggttgggt acatgatcta
     4261 aatgtttaat gctaaaggta tatcgtaagg gtagtgtttg tttttgaacg ataatttaga
     4321 agttctcata gaaagcgtat aacataggtc ttcagaaact ataaaagaat tttcatatag
     4381 tattaaaatc catagactaa aatctgagaa ttttttaaca tatgcaagtc agccaaacat
     4441 aagctaccaa aataaagagc aatgtgttct ggctgtttta tacttcaaca attttttccc
     4501 taagtggtaa gcaattactt taaaacatat ttttaaaaac atcggtatcg ggagctgcgg
     4561 tggctccggc cggttgtcct ggcacacaag gaggcgaggc tatgcgttcg aggccaacct
     4621 aggcaaaatt ggaaaaaaaa aaaaaaatca gtatcagaaa taatgcttga cataggattc
     4681 agtgttatac tctttggcac tttagagcag ccttttctct catttgaaat aaggtctttg
     4741 ttagccctac ctttactcag aaatttagtc ctactataaa aaattaggat tttaaaatat
     4801 aatgctgcct gtcatagtgt atttctaaat tgctctcttt gggagaaaaa cattattatt
     4861 gaattgagca tataactata taactatata actatatata tatatatata tatatatata
     4921 tatatatata tatatatata tatatatata tttttttttt tttttttttt tttttttttt
     4981 tgcccccgag acagagtctt actctgttgc ccaggctgga gtgcagtggc acgatcctgg
     5041 ttcactgcac cctctgcctc ccaggttcaa gcgattctcc tgcctcagcc tcccaagtag
     5101 ctggtactac aggtgcgtgc caccacacct ggcttatttt agttatttta gtagagatag
     5161 ggtttcacca tgttggccag gctggtctcg gaactcctga ctgcaggtga tccgtcctcc
     5221 ttgggctccc aaagtgctgg gattataggt gtgagccccc acgcctggcc aacttgatta
     5281 tattaatttt gggttttttg ttttgttttg ttttgttttt taatttttca aagaatttgt
     5341 ggatcttggt ataaagtcat tggaacatat ctgttttaac agcaagagat cctaggcaga
     5401 tacttcaaaa ggttaaaaat tcttaatcct acagaatttt aaatgaatct tatcaatgtt
     5461 ttgtaaacaa acaatatgaa tggccaaaaa attgccctcc attttactgg caggtaattt
     5521 atattgtctt acttaagaat tctcccctag tttttcaatt gtactttatc gtgttgtttt
     5581 caatgagata agtattttca tagggaaagc attttccagc ataatatttg cttgggtagc
     5641 atccgggttt tagtatttaa ccaagagcct tttaaatatt gaaaacccat agttcagaaa
     5701 atgttagtat tgctgccctt cttcacataa attttttttt aaattatact attattttgc
     5761 ttaattttat attgggttaa aacaaccttc aagaaggtta actaggaaag aagacctttt
     5821 tgttttattt ttactattta tatatagaag acaaatcagc atttggtgat agttttacat
     5881 gaccagttat caaacggtca tagtatgaag tgtgcagttg ttcattatta gtaaattatg
     5941 tttgattttt aaactattta gtactaatag ttgagatgaa aactgaagaa aaatgccaat
     6001 gtgacgtttg tgtatagcta gccttaaaaa acttcccatg tttttaggtg acttttttcc
     6061 ccctcttagt actctggaga aacaatgaag atgggccatc tcaattccag atgtaaacaa
     6121 aaagtaattt ttatttcaac atttaatgta actgctatta ttgtggattc ttgtcttgtg
     6181 tattttcttt cccttattca agtaatatag aataactttc cttaaaatga tttgatccaa
     6241 gatacgtcat ttctgtattg gcaaaatgcc actattaaag tgtaattctt gaatttaggt
     6301 tgaattgatg actttttaaa acaaaacaaa cactggacag ttctacattg tattgcgttt
     6361 gcgaatgtgc gtgaacacac gcacacgtgc aggagaatgt agtgccataa gaacactggc
     6421 gctttttaaa actttccaag ctagctactt attttcattt tcagggttga gtactctaag
     6481 ctctctactt actgtgagaa gttttctaca ttgtaaaatt aaaagattat atttaaaata
     6541 cttctttagg atgttattca gccatcaaaa aaaaacccaa ctaaaatgtc ttaccagtaa
     6601 agtattatta aaagcctcat ttgtggagat tcgctgactt ccttggttaa gctgtcttgt
     6661 atttacttaa tttgtcccct caaccaggtg ttttcttttc agcctgttac tgtcctgtgc
     6721 catttttaaa aatagccatc atgagtaagg gcttctttaa aataatccct gacaatatct
     6781 ttggcattaa tacgacataa agacaagaca gtatatgtat gttttcattc ctgttttgta
     6841 cgagtgcctc attttgtagt catgaaagta acatagtacc ttaaataaaa atgtaatgac
     6901 taatctaagg tctaggaata actattgtag aagctaagta gaacacttag tttggggtgt
     6961 caattttcta tcaacgcaaa caccttaggg tacccagacc aattttatgt atatatattc
     7021 tgaaatgctc cctttttaat gtggtcattt gttgccttct aagttgagag gacaaaactg
     7081 aataaagtga atgttaattg tagtctagtt tttttgtttg tttttgtgtt attgcaagat
     7141 tgctttaaga ttgcagaaat atgcattatt agccagcata gataatccag atgttataat
     7201 aggaaggaaa aacaactcca gttaaatgtg actgtggcga ctccctttta tgtatacatt
     7261 taaatgcata gttgcagagg acacatccct ccccttctgt ctccctaact tgaactgtag
     7321 accgtcctca ctgggaagag taaggcccca cattttgtgc aatagcaagc tactgcccac
     7381 ttctgcatgt ctgctacgag atcagaaagt cagtttcact aaggttgtct tttagctgaa
     7441 tacttagagt aaaaccaatc aaatccattg tacatacctg accatatgtc ccagataaaa
     7501 aggaaagact tgcgtgatgt ttttttcctt ctgagtgccc tgaggagatc cttttgcttc
     7561 agactatttc taatttttct ttgtttatag gttcagaatt ttttccatga ttgttttccc
     7621 ccaaacaagt gcatttttaa ttactttaaa tgcaggggtg atagaggaaa tgctattcga
     7681 accatagaca tctttaatct gtgaagctga aatttttcag caaaggaaat tgcatggtca
     7741 agttcagaaa ctggccatta atttccccct tccgctccac agttcatagc tactcttgtt
     7801 gcaaacatgt agtgataagg agaactaacg tatcaagggg ctgagagggt aacgttcccc
     7861 tcctcctcca tgtttttatt ttggtgtctg ttttgtttct agcactgtat ttagtactgt
     7921 agttgaatga ggtatgatgt ctcccttcaa ggaactcaga atggaacaga agcatgagca
     7981 aaagattgta ttacagaggt atgaataaaa tgccatgggg aacacagaga aaggttcacc
     8041 cgggagagat ggcctagcca cttccttagt gactgtttgg tatatgtagg tccccctgcc
     8101 attactcttt cagccttctg caatctagtt ctacttagtc acacacttct ctaagaccac
     8161 tcatacgtaa acactacgta gaggcccctt tttgcctcat tttacattgt ttagttatca
     8221 ttttgaaact tttcttcaca tatgtaacag tgccggagtt tttctgcttc tctgtgtttg
     8281 ttcagtaact cttctttagg atacacctaa agatgagaag cttcataccc agtactcctc
     8341 ttcattcact catatgtttt tgggatcagt cccttctgct ggctgtgcat tggtctaatg
     8401 gaacagaata gagtccagaa ataacccaac atgtatgtgg acaactggtt tttgacagag
     8461 gtgcaaaggt cttgaaaaaa tgatgctgga ataattgggc atcagatgca aaattaaaaa
     8521 caaattgatc catatcttaa cactggcaaa gattaaagtc caaatggatt atagttcccc
     8581 aaaactgtat aatttctaga agacaacaag gaaaacgtgt tcagccttgg gttaggaaaa
     8641 gatttcttaa atccaacacc aaaagcacaa tccatgaagg aaaaatcgat aaattgtact
     8701 ttatcaaaat tgagaacttc tctttgaaag gtaccataag gagaacaaaa agacaagctg
     8761 tagagtggca gaaaaatatt tgcaaaacat ttctgataaa tgagttgcat ctagattaca
     8821 taaagaagtc tcaaaactga acaagtaaac ccattgttta cttaatcgct gtttctcctg
     8881 agcttgctgc ctctgcccct gctctctctc cttttccatt tgttttcaac attgaatcca
     8941 gaatgttctt cttgagatcc aagtcagatc acaccaaccc tcagaactct ccaatagacg
     9001 accatggcac tcaaaagtcc acaatagcct tcaatgctgg gcaaaacatg aagcacccct
     9061 tttctccctt ctctgacccc atcacctctg tgttcaccct gctcctgccg tcctccctgc
     9121 ctccaaaaca ggtcaggcct tcgtgccttt gcacttacta tttgcaatac ccaaatgttc
     9181 ttcaggctct ttagcctctt catttctttc ctgaagtgtc attctcactg aggcttatct
     9241 aaagctgcag ctactggggc attcctgtct catctccctg ctgtattttg tactcccggc
     9301 tctcttttgt acttttaaac atactatatg gtttaccttt gttgtttata tttgcatgtt
     9361 gtttcccact tgaatgtaag ctccaaagat tttatttttt taaactgaat tattactgta
     9421 ttcccagaac aattccctgg caaatatttg gtactcaata gtaatgctaa gttagtaaat
     9481 aaatgatgaa tttagaatca aaataatgtg tctatggcca aaataaaacc tgaaatccct
     9541 gtcctatttc ccagaggtaa ctgctgttaa tagtttagtt gtgtgcttcc agacatacct
     9601 tcacagaatc atttatcaca ataaaggtgt catactatgc a
//
