LOCUS       NM_012325               2562 bp    mRNA    linear   PRI 11-FEB-2025
DEFINITION  Homo sapiens microtubule associated protein RP/EB family member 1
            (MAPRE1), mRNA.
ACCESSION   NM_012325
VERSION     NM_012325.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2562)
  AUTHORS   Gomez-Moron,A., Requena,S., Pertusa,C., Lozano-Prieto,M.,
            Calzada-Fraile,D., Scagnetti,C., Sanchez-Garcia,I.,
            Calero-Garcia,A.A., Izquierdo,M. and Martin-Cofreces,N.B.
  TITLE     End-binding protein 1 regulates the metabolic fate of CD4+ T
            lymphoblasts and Jurkat T cells and the organization of the
            mitochondrial network
  JOURNAL   Front Immunol 14, 1197289 (2023)
   PUBMED   37520527
  REMARK    GeneRIF: End-binding protein 1 regulates the metabolic fate of
            CD4[+] T lymphoblasts and Jurkat T cells and the organization of
            the mitochondrial network.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2562)
  AUTHORS   Radhakrishnan,R.M., Kizhakkeduth,S.T., Nair,V.M., Ayyappan,S.,
            Lakshmi,R.B., Babu,N., Prasannajith,A., Umeda,K., Vijayan,V.,
            Kodera,N. and Manna,T.K.
  TITLE     Kinetochore-microtubule attachment in human cells is regulated by
            the interaction of a conserved motif of Ska1 with EB1
  JOURNAL   J Biol Chem 299 (2), 102853 (2023)
   PUBMED   36592928
  REMARK    GeneRIF: Kinetochore-microtubule attachment in human cells is
            regulated by the interaction of a conserved motif of Ska1 with EB1.
REFERENCE   3  (bases 1 to 2562)
  AUTHORS   Dema,A., Charafeddine,R., Rahgozar,S., van Haren,J. and Wittmann,T.
  TITLE     Growth cone advance requires EB1 as revealed by genomic replacement
            with a light-sensitive variant
  JOURNAL   Elife 12, e84143 (2023)
   PUBMED   36715499
  REMARK    GeneRIF: Growth cone advance requires EB1 as revealed by genomic
            replacement with a light-sensitive variant.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2562)
  AUTHORS   Song,X., Yang,F., Yang,T., Wang,Y., Ding,M., Li,L., Xu,P., Liu,S.,
            Dai,M., Chi,C., Xiang,S., Xu,C., Li,D., Wang,Z., Li,L., Hill,D.L.,
            Fu,C., Yuan,K., Li,P., Zang,J., Hou,Z., Jiang,K., Shi,Y., Liu,X.
            and Yao,X.
  TITLE     Phase separation of EB1 guides microtubule plus-end dynamics
  JOURNAL   Nat Cell Biol 25 (1), 79-91 (2023)
   PUBMED   36536176
  REMARK    GeneRIF: Phase separation of EB1 guides microtubule plus-end
            dynamics.
            Erratum:[Nat Cell Biol. 2024 Jan;26(1):168-169. doi:
            10.1038/s41556-023-01324-4.. PMID: 38062156]
REFERENCE   5  (bases 1 to 2562)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   6  (bases 1 to 2562)
  AUTHORS   Juwana,J.P., Henderikx,P., Mischo,A., Wadle,A., Fadle,N.,
            Gerlach,K., Arends,J.W., Hoogenboom,H., Pfreundschuh,M. and
            Renner,C.
  TITLE     EB/RP gene family encodes tubulin binding proteins
  JOURNAL   Int J Cancer 81 (2), 275-284 (1999)
   PUBMED   10188731
REFERENCE   7  (bases 1 to 2562)
  AUTHORS   Morrison,E.E., Wardleworth,B.N., Askham,J.M., Markham,A.F. and
            Meredith,D.M.
  TITLE     EB1, a protein which interacts with the APC tumour suppressor, is
            associated with the microtubule cytoskeleton throughout the cell
            cycle
  JOURNAL   Oncogene 17 (26), 3471-3477 (1998)
   PUBMED   10030671
REFERENCE   8  (bases 1 to 2562)
  AUTHORS   Berrueta,L., Kraeft,S.K., Tirnauer,J.S., Schuyler,S.C., Chen,L.B.,
            Hill,D.E., Pellman,D. and Bierer,B.E.
  TITLE     The adenomatous polyposis coli-binding protein EB1 is associated
            with cytoplasmic and spindle microtubules
  JOURNAL   Proc Natl Acad Sci U S A 95 (18), 10596-10601 (1998)
   PUBMED   9724749
REFERENCE   9  (bases 1 to 2562)
  AUTHORS   Su,L.K., Burrell,M., Hill,D.E., Gyuris,J., Brent,R., Wiltshire,R.,
            Trent,J., Vogelstein,B. and Kinzler,K.W.
  TITLE     APC binds to the novel protein EB1
  JOURNAL   Cancer Res 55 (14), 2972-2977 (1995)
   PUBMED   7606712
REFERENCE   10 (bases 1 to 2562)
  AUTHORS   Golsteyn,R.M., Mundt,K.E., Fry,A.M. and Nigg,E.A.
  TITLE     Cell cycle regulation of the activity and subcellular localization
            of Plk1, a human protein kinase implicated in mitotic spindle
            function
  JOURNAL   J Cell Biol 129 (6), 1617-1628 (1995)
   PUBMED   7790358
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BJ992159.1, BC106068.1 and
            BM983860.1.
            
            On Nov 23, 2018 this sequence version replaced NM_012325.2.
            
            Summary: The protein encoded by this gene was first identified by
            its binding to the APC protein which is often mutated in familial
            and sporadic forms of colorectal cancer. This protein localizes to
            microtubules, especially the growing ends, in interphase cells.
            During mitosis, the protein is associated with the centrosomes and
            spindle microtubules. The protein also associates with components
            of the dynactin complex and the intermediate chain of cytoplasmic
            dynein. Because of these associations, it is thought that this
            protein is involved in the regulation of microtubule structures and
            chromosome stability. This gene is a member of the RP/EB family.
            [provided by RefSeq, Jul 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC106068.1, SRR1803613.177703.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000375571.6/ ENSP00000364721.5
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-9                 BJ992159.1         62-70
            10-2388             BC106068.1         1-2379
            2389-2562           BM983860.1         18-191              c
FEATURES             Location/Qualifiers
     source          1..2562
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="20"
                     /map="20q11.21"
     gene            1..2562
                     /gene="MAPRE1"
                     /gene_synonym="EB1"
                     /note="microtubule associated protein RP/EB family member
                     1"
                     /db_xref="GeneID:22919"
                     /db_xref="HGNC:HGNC:6890"
                     /db_xref="MIM:603108"
     exon            1..75
                     /gene="MAPRE1"
                     /gene_synonym="EB1"
                     /inference="alignment:Splign:2.1.0"
     exon            76..199
                     /gene="MAPRE1"
                     /gene_synonym="EB1"
                     /inference="alignment:Splign:2.1.0"
     CDS             79..885
                     /gene="MAPRE1"
                     /gene_synonym="EB1"
                     /note="adenomatous polyposis coli-binding protein EB1;
                     end-binding protein 1; APC-binding protein EB1"
                     /codon_start=1
                     /product="microtubule-associated protein RP/EB family
                     member 1"
                     /protein_id="NP_036457.1"
                     /db_xref="CCDS:CCDS13208.1"
                     /db_xref="GeneID:22919"
                     /db_xref="HGNC:HGNC:6890"
                     /db_xref="MIM:603108"
                     /translation="MAVNVYSTSVTSDNLSRHDMLAWINESLQLNLTKIEQLCSGAAY
                     CQFMDMLFPGSIALKKVKFQAKLEHEYIQNFKILQAGFKRMGVDKIIPVDKLVKGKFQ
                     DNFEFVQWFKKFFDANYDGKDYDPVAARQGQETAVAPSLVAPALNKPKKPLTSSSAAP
                     QRPISTQRTAAAPKAGPGVVRKNPGVGNGDDEAAELMQQVNVLKLTVEDLEKERDFYF
                     GKLRNIELICQENEGENDPVLQRIVDILYATDEGFVIPDEGGPQEEQEEY"
     exon            200..345
                     /gene="MAPRE1"
                     /gene_synonym="EB1"
                     /inference="alignment:Splign:2.1.0"
     exon            346..553
                     /gene="MAPRE1"
                     /gene_synonym="EB1"
                     /inference="alignment:Splign:2.1.0"
     exon            554..675
                     /gene="MAPRE1"
                     /gene_synonym="EB1"
                     /inference="alignment:Splign:2.1.0"
     exon            676..828
                     /gene="MAPRE1"
                     /gene_synonym="EB1"
                     /inference="alignment:Splign:2.1.0"
     exon            829..2562
                     /gene="MAPRE1"
                     /gene_synonym="EB1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1318..1323
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MAPRE1"
                     /gene_synonym="EB1"
                     /note="hexamer: AATATA"
     regulatory      1320..1325
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MAPRE1"
                     /gene_synonym="EB1"
                     /note="hexamer: TATAAA"
     polyA_site      1336
                     /gene="MAPRE1"
                     /gene_synonym="EB1"
     polyA_site      1341
                     /gene="MAPRE1"
                     /gene_synonym="EB1"
                     /note="major polyA site"
     regulatory      2056..2061
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MAPRE1"
                     /gene_synonym="EB1"
                     /note="hexamer: ACTAAA"
     regulatory      2083..2088
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MAPRE1"
                     /gene_synonym="EB1"
                     /note="hexamer: AATACA"
     polyA_site      2084
                     /gene="MAPRE1"
                     /gene_synonym="EB1"
     polyA_site      2106
                     /gene="MAPRE1"
                     /gene_synonym="EB1"
     regulatory      2306..2311
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MAPRE1"
                     /gene_synonym="EB1"
                     /note="hexamer: GATAAA"
     polyA_site      2327
                     /gene="MAPRE1"
                     /gene_synonym="EB1"
     regulatory      2538..2543
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MAPRE1"
                     /gene_synonym="EB1"
                     /note="hexamer: AATAAA"
     polyA_site      2562
                     /gene="MAPRE1"
                     /gene_synonym="EB1"
ORIGIN      
        1 gtctggcggt ttgaacgaga cgaagacgga accggagccg gttgcgggca gtggacgcgg
       61 ttctgccgag agccgaagat ggcagtgaac gtatactcaa cgtcagtgac cagtgataac
      121 ctaagtcgac atgacatgct ggcctggatc aatgagtctc tgcagttgaa tctgacaaag
      181 atcgaacagt tgtgctcagg ggctgcgtat tgtcagttta tggacatgct gttccctggc
      241 tccattgcct tgaagaaagt gaaattccaa gctaagctag aacacgagta catccagaac
      301 ttcaaaatac tacaagcagg ttttaagaga atgggtgttg acaaaataat tcctgtggac
      361 aaattagtaa aaggaaagtt tcaggacaat tttgaattcg ttcagtggtt caagaagttt
      421 ttcgatgcaa actatgatgg aaaagactat gaccctgtgg ctgccagaca aggtcaagaa
      481 actgcagtgg ctccttccct tgttgctcca gctctgaata aaccgaagaa acctctcact
      541 tctagcagtg cagctcccca gaggcccatc tcaacacaga gaaccgctgc ggctcctaag
      601 gctggccctg gtgtggtgcg aaagaaccct ggtgtgggca acggagacga cgaggcagct
      661 gagttgatgc agcaggtcaa cgtattgaaa cttactgttg aagacttgga gaaagagagg
      721 gatttctact tcggaaagct acggaacatt gaattgattt gccaggagaa cgagggggaa
      781 aacgaccctg tattgcagag gattgtagac attctgtatg ccacagatga aggctttgtg
      841 atacctgatg aagggggccc acaggaggag caagaagagt attaacagcc tggaccagca
      901 gagcaacatc ggaattcttc actccaaatc atgtgcttaa ctgtaaaata ctcccttttg
      961 ttatccttag aggactcact ggtttctttt cataagcaaa aagtacctct tcttaaagtg
     1021 cactttgcag acgtttcact ccttttccaa taagtttgag ttaggagctt ttaccttgta
     1081 gcagagcagt attaacacct agttggttca cctggaaaac agagaggctg accgtggggc
     1141 tcaccatgcg gatgcgggtc acactgaatg ctggagagat gttatgtaat atgctgaggt
     1201 ggcgacctca gtggagaaat gtaaagactg aattgaattt taagctaatg tgaaatcaga
     1261 gaatgttgta ataagtaaat gccttaagag tatttaaaat atgcttccac atttcaaaat
     1321 ataaaatgta acatgacaag agattttgcg tttgacattg tgtctgggaa ggaagggcca
     1381 gaccttggaa cctttggaac ctgctgtcaa caggtcttac agggctgctt gaaccctcat
     1441 aggcctaggc tttggtctaa aaggaacatt taaaaagttg ccctgtaaag ttatttggtg
     1501 tcattgacca attgcatccc agctaaaaag caagaggcat cgttgcctgg ataatagagg
     1561 atgtgtttca gccctgagat gttacagttg aagagcttgg ttttcattga gcatttctct
     1621 atttttccag ttatccccga aatttctatg tattatattt tttggggaag tgaggtgtgc
     1681 ccagtttttt aatctaacaa ctacttttgg ggacttgccc acatctctgg gatttgaatg
     1741 gggattgtat cccattttac tgtcttttag gtttacattt accacgtttc tcttctctgc
     1801 tccccttgcc cactggggac tcctctttgg ctccttgaag tttgctgctt agagttggaa
     1861 gtgcagcagg caggtgatca tgctgcaagt tctttctgga cctctggcaa agggagtggt
     1921 cagtgaaggc catcgttacc ttgggatctg ccaggctggg gtgttttcgg tatctgctgt
     1981 tcacagctct ccactgtaat ccgaatactt tgccagtgca ctaatctctt tggagataaa
     2041 attcattagt gtgttactaa atgttaattt tcttttgcgg aaaatacagt accgtgtctg
     2101 aattaattat taatatttaa aatacttcat tccttaactc tccctcattt gctttgccca
     2161 cagcctattc agttcctttg tttggcagga ttctgcaaaa tgtgtctcac ccactactga
     2221 gattgttcag cccctgatgt atttgtattg atttgtttct ggtggtagct tgtcctgaaa
     2281 tgtgtgtaga aagcaagtat tttatgataa aaatgttgtg tagtgcatgc tctgtgtgga
     2341 attcagagga aaacccagat tcagtgatta acaatgccaa aaaatgcaag taactagcca
     2401 ttgttcaaat gacagtggtg ctatttctct tttgtggcct tttagacttt tgttgcccta
     2461 aaattccatt ttattgggaa cccattttcc acctggtctt tcttgacagg gtttttttct
     2521 actttaaaca gtttctaaat aaaattctgt atttcaagag ta
//
