LOCUS       NM_003668              11083 bp    mRNA    linear   PRI 09-JUN-2024
DEFINITION  Homo sapiens MAPK activated protein kinase 5 (MAPKAPK5), transcript
            variant 1, mRNA.
ACCESSION   NM_003668
VERSION     NM_003668.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 11083)
  AUTHORS   Maroofian,R., Efthymiou,S., Suri,M., Rahman,F., Zaki,M.S.,
            Maqbool,S., Anwa,N., Ruiz-Perez,V.L., Yanovsky-Dagan,S.,
            Elpeleg,O., Sudhakar,S., Mankad,K., Harel,T. and Houlden,H.
  TITLE     Consolidating the association of biallelic MAPKAPK5 pathogenic
            variants with a distinct syndromic neurodevelopmental disorder
  JOURNAL   J Med Genet 60 (8), 791-796 (2023)
   PUBMED   36581449
  REMARK    GeneRIF: Consolidating the association of biallelic MAPKAPK5
            pathogenic variants with a distinct syndromic neurodevelopmental
            disorder.
REFERENCE   2  (bases 1 to 11083)
  AUTHORS   Vecchio,D., Cocciadiferro,D., Macchiaiolo,M., Gonfiantini,M.V.,
            Agolini,E., Matraxia,M., Carboni,A., Coretti,A., Villani,A.,
            Panfili,F.M., Dentici,M.L., Buonuomo,P.S., Rana,I., Colafati,G.S.,
            Digilio,M.C., Novelli,A. and Bartuli,A.
  TITLE     Expanding the novel MAPKAPK5-related developmental disorder's
            genotype-phenotype correlation: Patient report and 19 months of
            follow-up
  JOURNAL   Clin Genet 102 (2), 142-148 (2022)
   PUBMED   35575217
  REMARK    GeneRIF: Expanding the novel MAPKAPK5-related developmental
            disorder's genotype-phenotype correlation: Patient report and 19
            months of follow-up.
REFERENCE   3  (bases 1 to 11083)
  AUTHORS   Khalil,M.I., Singh,V., King,J. and De Benedetti,A.
  TITLE     TLK1-mediated MK5-S354 phosphorylation drives prostate cancer cell
            motility and may signify distinct pathologies
  JOURNAL   Mol Oncol 16 (13), 2537-2557 (2022)
   PUBMED   35064619
  REMARK    GeneRIF: TLK1-mediated MK5-S354 phosphorylation drives prostate
            cancer cell motility and may signify distinct pathologies.
REFERENCE   4  (bases 1 to 11083)
  AUTHORS   Wang,Y., Xie,Y., Dong,B., Xue,W., Chen,S., Mitsuo,S., Zou,H.,
            Feng,Y., Ma,K., Dong,Q., Cao,J. and Zhu,C.
  TITLE     The TTYH3/MK5 Positive Feedback Loop regulates Tumor Progression
            via GSK3-beta/beta-catenin signaling in HCC
  JOURNAL   Int J Biol Sci 18 (10), 4053-4070 (2022)
   PUBMED   35844789
  REMARK    GeneRIF: The TTYH3/MK5 Positive Feedback Loop regulates Tumor
            Progression via GSK3-beta/beta-catenin signaling in HCC.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 11083)
  AUTHORS   Peng,Z., Ouyang,X., Wang,Y. and Fan,Q.
  TITLE     MAPKAPK5-AS1 drives the progression of hepatocellular carcinoma via
            regulating miR-429/ZEB1 axis
  JOURNAL   BMC Mol Cell Biol 23 (1), 21 (2022)
   PUBMED   35468721
  REMARK    GeneRIF: MAPKAPK5-AS1 drives the progression of hepatocellular
            carcinoma via regulating miR-429/ZEB1 axis.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 11083)
  AUTHORS   Sudo,T., Maruyama,M. and Osada,H.
  TITLE     p62 functions as a p38 MAP kinase regulator
  JOURNAL   Biochem Biophys Res Commun 269 (2), 521-525 (2000)
   PUBMED   10708586
REFERENCE   7  (bases 1 to 11083)
  AUTHORS   New,L., Jiang,Y., Zhao,M., Liu,K., Zhu,W., Flood,L.J., Kato,Y.,
            Parry,G.C. and Han,J.
  TITLE     PRAK, a novel protein kinase regulated by the p38 MAP kinase
  JOURNAL   EMBO J 17 (12), 3372-3384 (1998)
   PUBMED   9628874
REFERENCE   8  (bases 1 to 11083)
  AUTHORS   Borsch-Haubold,A.G., Bartoli,F., Asselin,J., Dudler,T.,
            Kramer,R.M., Apitz-Castro,R., Watson,S.P. and Gelb,M.H.
  TITLE     Identification of the phosphorylation sites of cytosolic
            phospholipase A2 in agonist-stimulated human platelets and HeLa
            cells
  JOURNAL   J Biol Chem 273 (8), 4449-4458 (1998)
   PUBMED   9468497
REFERENCE   9  (bases 1 to 11083)
  AUTHORS   Ni,H., Wang,X.S., Diener,K. and Yao,Z.
  TITLE     MAPKAPK5, a novel mitogen-activated protein kinase (MAPK)-activated
            protein kinase, is a substrate of the extracellular-regulated
            kinase (ERK) and p38 kinase
  JOURNAL   Biochem Biophys Res Commun 243 (2), 492-496 (1998)
   PUBMED   9480836
REFERENCE   10 (bases 1 to 11083)
  AUTHORS   de Carvalho,M.G., McCormack,A.L., Olson,E., Ghomashchi,F.,
            Gelb,M.H., Yates,J.R. 3rd and Leslie,C.C.
  TITLE     Identification of phosphorylation sites of human 85-kDa cytosolic
            phospholipase A2 expressed in insect cells and present in human
            monocytes
  JOURNAL   J Biol Chem 271 (12), 6987-6997 (1996)
   PUBMED   8636128
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC003029.5.
            
            On Jul 16, 2019 this sequence version replaced NM_003668.3.
            
            Summary: The protein encoded by this gene is a tumor suppressor and
            member of the serine/threonine kinase family. In response to
            cellular stress and proinflammatory cytokines, this kinase is
            activated through its phosphorylation by MAP kinases including
            MAPK1/ERK, MAPK14/p38-alpha, and MAPK11/p38-beta. The encoded
            protein is found in the nucleus but translocates to the cytoplasm
            upon phosphorylation and activation. This kinase phosphorylates
            heat shock protein HSP27 at its physiologically relevant sites. Two
            alternately spliced transcript variants of this gene encoding
            distinct isoforms have been reported. [provided by RefSeq, Nov
            2012].
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK122767.1, BC041049.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000550735.7/ ENSP00000449667.2
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-542               AC003029.5         51010-51551
            543-616             AC003029.5         74032-74105
            617-692             AC003029.5         74938-75013
            693-790             AC003029.5         76354-76451
            791-899             AC003029.5         77535-77643
            900-989             AC003029.5         79053-79142
            990-1085            AC003029.5         79867-79962
            1086-1166           AC003029.5         89229-89309
            1167-1354           AC003029.5         92363-92550
            1355-1475           AC003029.5         94698-94818
            1476-1606           AC003029.5         97270-97400
            1607-1722           AC003029.5         97667-97782
            1723-1827           AC003029.5         98822-98926
            1828-11083          AC003029.5         101749-111004
FEATURES             Location/Qualifiers
     source          1..11083
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="12"
                     /map="12q24.12-q24.13"
     gene            1..11083
                     /gene="MAPKAPK5"
                     /gene_synonym="MAPKAP-K5; MK-5; MK5; NCFD; PRAK"
                     /note="MAPK activated protein kinase 5"
                     /db_xref="GeneID:8550"
                     /db_xref="HGNC:HGNC:6889"
                     /db_xref="MIM:606723"
     exon            1..542
                     /gene="MAPKAPK5"
                     /gene_synonym="MAPKAP-K5; MK-5; MK5; NCFD; PRAK"
                     /inference="alignment:Splign:2.1.0"
     CDS             507..1922
                     /gene="MAPKAPK5"
                     /gene_synonym="MAPKAP-K5; MK-5; MK5; NCFD; PRAK"
                     /EC_number="2.7.11.1"
                     /note="isoform 1 is encoded by transcript variant 1;
                     p38-regulated/activated protein kinase; MAP
                     kinase-activated protein kinase 5; MAPKAPK-5; MAPKAP
                     kinase 5; mitogen-activated protein kinase-activated
                     protein kinase 5"
                     /codon_start=1
                     /product="MAP kinase-activated protein kinase 5 isoform 1"
                     /protein_id="NP_003659.2"
                     /db_xref="CCDS:CCDS44976.1"
                     /db_xref="GeneID:8550"
                     /db_xref="HGNC:HGNC:6889"
                     /db_xref="MIM:606723"
                     /translation="MSEESDMDKAIKETSILEEYSINWTQKLGAGISGPVRVCVKKST
                     QERFALKILLDRPKARNEVRLHMMCATHPNIVQIIEVFANSVQFPHESSPRARLLIVM
                     EMMEGGELFHRISQHRHFTEKQASQVTKQIALALRHCHLLNIAHRDLKPENLLFKDNS
                     LDAPVKLCDFGFAKIDQGDLMTPQFTPYYVAPQVLEAQRRHQKEKSGIIPTSPTPYTY
                     NKSCDLWSLGVIIYVMLCGYPPFYSKHHSRTIPKDMRRKIMTGSFEFPEEEWSQISEM
                     AKDVVRKLLKVKPEERLTIEGVLDHPWLNSTEALDNVLPSAQLMMDKAVVAGIQQAHA
                     EQLANMRIQDLKVSLKPLHSVNNPILRKRKLLGTKPKDSVYIHDHENGAEDSNVALEK
                     LRDVIAQCILPQAGENEDEKLNEVMQEAWKYNRECKLLRDTLQSFSWNGRGFTDKVDR
                     LKLAEIVKQVIEEQTTSHESQ"
     exon            543..616
                     /gene="MAPKAPK5"
                     /gene_synonym="MAPKAP-K5; MK-5; MK5; NCFD; PRAK"
                     /inference="alignment:Splign:2.1.0"
     exon            617..692
                     /gene="MAPKAPK5"
                     /gene_synonym="MAPKAP-K5; MK-5; MK5; NCFD; PRAK"
                     /inference="alignment:Splign:2.1.0"
     exon            693..790
                     /gene="MAPKAPK5"
                     /gene_synonym="MAPKAP-K5; MK-5; MK5; NCFD; PRAK"
                     /inference="alignment:Splign:2.1.0"
     exon            791..899
                     /gene="MAPKAPK5"
                     /gene_synonym="MAPKAP-K5; MK-5; MK5; NCFD; PRAK"
                     /inference="alignment:Splign:2.1.0"
     exon            900..989
                     /gene="MAPKAPK5"
                     /gene_synonym="MAPKAP-K5; MK-5; MK5; NCFD; PRAK"
                     /inference="alignment:Splign:2.1.0"
     exon            990..1085
                     /gene="MAPKAPK5"
                     /gene_synonym="MAPKAP-K5; MK-5; MK5; NCFD; PRAK"
                     /inference="alignment:Splign:2.1.0"
     exon            1086..1166
                     /gene="MAPKAPK5"
                     /gene_synonym="MAPKAP-K5; MK-5; MK5; NCFD; PRAK"
                     /inference="alignment:Splign:2.1.0"
     exon            1167..1354
                     /gene="MAPKAPK5"
                     /gene_synonym="MAPKAP-K5; MK-5; MK5; NCFD; PRAK"
                     /inference="alignment:Splign:2.1.0"
     exon            1355..1475
                     /gene="MAPKAPK5"
                     /gene_synonym="MAPKAP-K5; MK-5; MK5; NCFD; PRAK"
                     /inference="alignment:Splign:2.1.0"
     exon            1476..1606
                     /gene="MAPKAPK5"
                     /gene_synonym="MAPKAP-K5; MK-5; MK5; NCFD; PRAK"
                     /inference="alignment:Splign:2.1.0"
     exon            1607..1722
                     /gene="MAPKAPK5"
                     /gene_synonym="MAPKAP-K5; MK-5; MK5; NCFD; PRAK"
                     /inference="alignment:Splign:2.1.0"
     exon            1723..1827
                     /gene="MAPKAPK5"
                     /gene_synonym="MAPKAP-K5; MK-5; MK5; NCFD; PRAK"
                     /inference="alignment:Splign:2.1.0"
     exon            1828..11083
                     /gene="MAPKAPK5"
                     /gene_synonym="MAPKAP-K5; MK-5; MK5; NCFD; PRAK"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2258..2263
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MAPKAPK5"
                     /gene_synonym="MAPKAP-K5; MK-5; MK5; NCFD; PRAK"
                     /note="hexamer: AGTAAA"
     polyA_site      2278
                     /gene="MAPKAPK5"
                     /gene_synonym="MAPKAP-K5; MK-5; MK5; NCFD; PRAK"
                     /note="major polyA site"
     regulatory      6748..6753
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MAPKAPK5"
                     /gene_synonym="MAPKAP-K5; MK-5; MK5; NCFD; PRAK"
                     /note="hexamer: ATTATA"
     polyA_site      6767
                     /gene="MAPKAPK5"
                     /gene_synonym="MAPKAP-K5; MK-5; MK5; NCFD; PRAK"
     regulatory      11058..11063
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MAPKAPK5"
                     /gene_synonym="MAPKAP-K5; MK-5; MK5; NCFD; PRAK"
                     /note="hexamer: AATAAA"
     polyA_site      11083
                     /gene="MAPKAPK5"
                     /gene_synonym="MAPKAP-K5; MK-5; MK5; NCFD; PRAK"
ORIGIN      
        1 gtcccggcga tgtgtggcgc tgaggcggcg gcgggagcag cggcgccgag ctctgcttcg
       61 gcttcggctt cggcttcgcg gcggtgcagg cggcggaggc ggaggcgcag gctcttcttt
      121 aggacctggc gagcccaggt ctaagcggcg gccccgcgag gcccttgcac ggcgccccgg
      181 gtcgaggccc tccccgcctc gccctacccc aggagcctgc ctccccagct ggggatgagg
      241 ctaggaggcg gccgcgtggg gcccagcaca aagacctgtc cccaggggcc gccgcctccg
      301 ccgctgctgc tgccgccagc ctagagccgc ccgccgaagc agagccggcg ccggggtcct
      361 catccccacc ggtcccgagg ggcggctgct gcccgtcgcc acgaggccca ggggcccgag
      421 tgccgagccc tttgctccct cggccgcgcg gggacagggc tgctgagcag cctccgcctc
      481 tcccggctgt gggggcccca ctgagtatgt cggaggagag cgacatggac aaagccatca
      541 aggaaacttc cattttagaa gaatacagta tcaattggac tcagaagctg ggagctggaa
      601 ttagtggtcc agttagagtc tgtgtaaaga aatctactca agaacggttt gcgctgaaaa
      661 ttcttcttga tcgtccaaaa gctagaaatg aggtacgtct gcacatgatg tgtgccacac
      721 acccaaacat agttcagatt attgaagtgt ttgctaacag tgtccagttt ccccatgagt
      781 ccagccctag ggcccgactc ttaattgtaa tggagatgat ggaaggggga gagctatttc
      841 acagaatcag ccagcaccgg cactttacag agaagcaagc cagccaagta acaaagcaga
      901 tagctttggc tctgcggcac tgtcacttgt taaacattgc gcacagagac ctcaagcctg
      961 aaaatctgct ttttaaggat aactctttgg atgccccagt gaagttgtgt gactttggat
     1021 ttgccaagat tgaccaaggt gacttgatga caccccagtt caccccttat tatgtagcac
     1081 cccaggtact ggaggcgcaa agaaggcatc agaaggagaa atctggcatc atacctacct
     1141 caccgacgcc ctacacttac aacaagagct gtgacttgtg gtccctaggg gtgattatct
     1201 atgtgatgct gtgcggatac cctccttttt actccaaaca ccacagccgg actatcccaa
     1261 aggatatgcg aagaaagatc atgacaggca gttttgagtt cccagaggaa gagtggagtc
     1321 agatctcaga gatggccaaa gatgttgtga ggaagctcct gaaggtcaaa ccggaggaga
     1381 gactcaccat cgagggagtg ctggaccacc cctggctcaa ttccaccgag gccctggata
     1441 atgtgctgcc ttctgctcag ctgatgatgg acaaggcagt ggttgcagga atccagcagg
     1501 ctcacgcgga acagttggcc aacatgagaa tccaggatct gaaagtcagc ctcaaacccc
     1561 tgcactcagt gaacaacccc attctgcgga agaggaagtt acttggcacc aagccaaagg
     1621 acagtgtcta tatccacgac catgagaatg gagccgagga ttccaatgtt gccttggaaa
     1681 aactccgaga tgtgattgct cagtgtattc tcccccaggc tggagagaat gaagatgaga
     1741 aactgaatga agtaatgcag gaggcttgga agtataaccg ggaatgcaaa ctcctaagag
     1801 atactctgca gagcttcagc tggaatggtc gtggattcac agataaagta gatcgactaa
     1861 aactggcaga aattgtgaag caggtgatag aagagcaaac cacgtcccac gaatcccaat
     1921 aatgacagct tcagactttg tttttttaac aatttgaaaa attattcttt aatgtataaa
     1981 gtaattttat gtaaattaat aaatcataat ttcatttcca cattgattaa agctgctgta
     2041 tagatttagg gtgcaggact taataatagt atagttattg tttgttttta agaaaagctc
     2101 agttctagag acatactatt actttaggac tgtgtagttg tatatttgta agatgacaga
     2161 tgatgctgtc aagcaatatt gttttatttg taataaaata tacaaaaatc acttgccagc
     2221 agtagaaaaa ggaccgacta taccgacctt tctgattagt aaacagttga atcaaggact
     2281 ctggattctg gtttcaattg ccctgtgtgt ttattttcca caccattgct agattagctc
     2341 tactgccaag acatttttgt ggctgtcatt gcagactgct tgctggaact cacaggaggc
     2401 acacttgagc ttggggagcc aacttccatg atggaagaag agggaggtga gccctcattt
     2461 ctcctgcccc ctaattccca agagaaaagc aggaacattt cctttttgtc atatctgtta
     2521 aaagggtact ggtgtgtttc ttaaatctaa cagggttttc ttttgggttt attctttttt
     2581 tttttttttt tttgagacag agtcttgctg ttgtagtgga ggctggagta cagtggcact
     2641 atctccgctc actgcaacct ccgcctccca ggttccagtg atcctcccac atcagcctcc
     2701 caagtagctg ggattacagg tgcccgccac cacgcctggc taatttttgt atttttagta
     2761 gagacagggt ttcaccacgt tggccaggct ggtcttgaac tcctgacctc aggtgatacg
     2821 tctgccttgg cctcccaaag tgctgggatt acaggcgtga gccactgtgc ccggccttgg
     2881 gtttattctt tctttatatg tttgtttgtt tgtttgtttt gagatggagt cttgctctta
     2941 tcacccaggg tggagtgcag tggcacgatc tcggctcact gcaacctctg tctcccaggt
     3001 tcaagtgatt ctcctgcctc agcctcccaa gtagttggga ttacaggcac ccgccaccat
     3061 gcccggctaa tttttgtatt tttagtagag acagggtttc accatgttgg ccaggctggt
     3121 ctcgaactcc tgaccatcca cctcagcctc tcaaagtact gggattacag gcatgagcca
     3181 ccaagcctgg cctattcttt atatgtttaa ccggagcttc cctttcccct tccaggaggt
     3241 cctgctttgt cctttcccct tccctgcagc cttcaccagc tccattactc cttatttctg
     3301 ttccctgttc ctctggtcct gtttttggaa taacttgtcc gagaaattta catataatta
     3361 attgaagtgg gactattgga ttatcatgta agatgatcca gttttggttt ttttttttat
     3421 aaggctgccc tgtttctctg taatatgtct gtctgggttt tgtgttggca tcctctgggc
     3481 tgcatttatc ttgtttatta cccttcattt tcaggaagca tggttttggc cccgagagca
     3541 gctgtttgaa ggaaaaataa ggctaggtcc ttttttagtt ttctgttgtg agtatgggtt
     3601 tccataacaa accaattttc gtgtatatgt gtgtgtgtgt gtgtgtgtgt gtgaagcagt
     3661 ttttggtggg tacttacgtt ggtacataga aaagctggca ggtagactcc agctcgcaga
     3721 gcagagggag aggatagtca tctgttattc aattttttct catctcattc tcttcgatca
     3781 aaattgatct caccagcaac ttattttcta actcctccac tgtgctgagt aattcctcca
     3841 ccctgaatct tcagggtgac atttggttag tatgccctgt atgtgtgtga ctgcctgtgg
     3901 gaatagatga taaaaaacaa gtgtcttaag aattacctta ttacattgct tccttttttt
     3961 tttttttttt tttttgagat ggattcttgc tctgtcgccc aggctggagt gcagtggcgc
     4021 gatcttggct cactgcaagc tctgcctccc aggttcacac cattctccta cctcagcctc
     4081 ctgagtagct gggactatac aggtgcccgc caccgcgccc ggctaatttc tttttgtatt
     4141 tttagtagag acagggtttc accgtgttag ccaggatggt cttgatctcc tgacctcgtg
     4201 atccgcccac ctcagcctcc aaaagtgctg ggattacagg tgtgagccac tgcacccagc
     4261 ctacattggt tcctttttat cttggagaat ttaattgaaa gatatttttt gagataaagt
     4321 tcactaatct taggtgactg ataactgtta taaatggatt tcctgtaagg agaatatgtc
     4381 tcccccccac cccccaccct gctgccacca agacagagtc tcgctctgtc ccccaggctg
     4441 gagtgcagtg gcttgctctt ggctcactgc aacctctgcc tcccaggttc aagcgattct
     4501 cgtgcctcag acttccaagt agctgggact acaggcacac accaccacgc ccagctaatt
     4561 tttgtgtttt tagtagagac ggggtttcat catattggcc aggctggtct caaactcctg
     4621 acctcgtgat ccgcccacct cggcctccca aagtgctggg attacaggcg tgagccaccg
     4681 tgcgcgacct gaatactttt catgatggca aaatctcacc ggcaacagaa gaaaaaccaa
     4741 tgtttttaaa tttactgacc agaaaacttg actgagagtt gaattaagtg cctgttgttg
     4801 tgtttttcag tgaaatgcaa atcttacaga tcacttctta cagttaggaa tactgcactt
     4861 gtttgtgtaa tattagaaca actgtagttt tcctatctta gggcagaggt tcagtattcc
     4921 atggggagaa aaaaacagta attatctttg ttactcttct tctttagagg aatattctag
     4981 catatccatt atatccataa cttcttaaat ttgtgacttg ggtgtcttct aaaaaggctc
     5041 aggatgatta ttttttcctt cagtgtatgc tgaattttag gtgcgatgga aaaggtatcc
     5101 aaagtgatac tccctttgac tctgagcatc agttgcccgt gtttcagtca cttcaagctg
     5161 aattcatgcc ctcaatgtaa gtgctgtgtc cagacacatc caaatgagca ccaagctgga
     5221 agtttgtgaa cactctggag gatgccccag ctgttgtaat gcaacagcat accttgaaaa
     5281 aaaacagtag ctcccaaggg actcagcatg gcttggtctt cgaagtggag ttttgtttgt
     5341 gtattcctag tccagcagta aattctgctt taacataact gtcaggtttg ctactcgaaa
     5401 tttgcacata actggaagtg aaacgatatc tttctcatat tttttggcct aacaaacacc
     5461 tatacctgtg gaaacataac actgtaaata taattcttat aaagcagcag gttatgctct
     5521 cctgtttgcc tacatgattt attaaccttc tgcttactaa agccattact ggtcaatcag
     5581 atacttttag actttaccac agttataaaa gtcagcttga tgtagtaaaa aaacttgtct
     5641 ctctctgact aatatgtatg aatataggag aatcccagtc tgaatttact gtttgaaaca
     5701 tagatgaggc tcagcgtggt ggctcatgcc tttagtaatc ccagcacttt gggaagctga
     5761 ggcgggcaga tcaccttagg tcaagagttc aagaccagct tggccaacat ggtgaaatgc
     5821 cgtctctact aaaaatacaa aaattagcca ggcatggtgg cacatgcctg taatcccagc
     5881 tgctcgggag gctgaggcag gagaattgct tgaacctggg aggcagaggt tgcagtgagt
     5941 ggagatcgca ccattgcact ccagcctggg tgacagagca aaactccgtc tcagaaataa
     6001 aaaagagacg tgatcctgct atgttgccca agttagactc aagctcctgg gctcaagcaa
     6061 tcctcctgcc tcagccttct aagtagctgg gactacagga gcaaaccact gtgcctggca
     6121 ggaattactc tttaaaaaga gtttatgttg cttgtattca tttgagaaaa aatgagttga
     6181 agaaatggta attgttggtc aaagaagcca aaagatttaa ataaggtctt gtgtgtgggt
     6241 atgtgtatgt gcatgtattt attgaagaaa ataagtttca atttgagaat tagaaaattg
     6301 gatatttcat tttaattatt tatttaaaga gtagagtgtt cttccattat tgaatcttct
     6361 tttatcccac caaatggctt aacctaattt gcgctgccta ttttggttaa gaaacaggga
     6421 aacaccctaa aatctgtgat aattttcaaa atacaatttt aagaagcttt gcgcttacat
     6481 aaatcacaag aaatacagct ggaaatcatt tcacttttct tgctgtaaat ttttttagtg
     6541 ggtttgtgac agtataaatt ttcttgctag atgctaatga tacattgtat tatctgtatt
     6601 aggaattaaa tatttaatag gaatagtgaa taaattgatc tgctctcatg ctgttatcta
     6661 agatcttatg ctaccattac attcctttct gtagtcgtat ctcatatttg aaaggtctca
     6721 tgttgtaaat atttgacttg ctgtaaaatt atacaagata aaaaatgtag agttttattt
     6781 ttcttcatct agaatgcaca gaataactgc gtaaggaatg ggtgcatagg gcttcttttc
     6841 cttttaaatt tgtactgcta aatacaccaa ggaaattgac atcaaaacca aattcaagga
     6901 cttccacaga catttcaggt caattttagt tccaaaccga agaaatcttt taaaagttct
     6961 tatatagccc agttttcttt tcttgatgaa atgttaacta gacacctggt tagtcagctt
     7021 ttattttgac cccacagttt tttttttgag acggattctc actctgttgc ccaggttgga
     7081 gtgcaatggt gtgatctcgg cttactgcaa cctacgcctc ctgggttcaa gcgattctcc
     7141 tgcctcacct tcctgagtag ctgagattac aggcgcctgc caccacgccc cgctaatttt
     7201 tgcattttta gtagggacag ggtttcacca tgctggccag gctggtctcg aactcccgac
     7261 ctcaggtgat ccgcctgcct cggcctccca aagtgctggg attacagggg tgagccaccg
     7321 tgcccgggcc ccacactttg tttttatgtt agggcagttg gaggaactga actttgatcc
     7381 tgatccttta tatactccca tggtgaaact tgagcccatt ttacttaaca caatatatcc
     7441 gtgcattagg aaaacttggt agagaagatt atatgcagat ataaaatatc taaactcatt
     7501 tgttatgaac caacgataat gagggaaaac ttggacatcc acatacagat tttcttgaat
     7561 taaagaaaaa aaccagacac tgacaaagga gagctgaact gataaatggt accctgaaaa
     7621 ctgcagaaat gagggaaaga ggcagtgggc cacaaagaac tttgtccttt tcagagatcc
     7681 ccagagaagc cctgccccct tccccccacc ccctgacaga atgttgtgtt tcaaagaatg
     7741 agcagattaa tggatatcaa gttgtgtggc ctgagctgag gataaagaat aatttgcctg
     7801 aggagttaca gtactggctg gatcactaca ggccttctac ttggaatatc ttcccatgaa
     7861 agtaaaagga atataactaa ggaagattaa agttgttgct gttggaagga agacatacta
     7921 aagaaactgc cgaaggccag gagagaagca ggaagagaaa cacggcttga agttctgtaa
     7981 gaatcagagt tgggcaagaa ctgccaataa gggagccagc gtagatctga gtactaaaca
     8041 cctgagcctc cgagggtttg tgtgcgaggg agagctctgc tgatggacat aggcccagaa
     8101 tcattaactg atttatttgg gccatgtcac tggtggcatt attaaaagac tctgccaaca
     8161 tcctgcttta tggaaaaacc aagtggcctt ggaagaggct aagataaagt ttcaaacttg
     8221 ggctccacag aagtggaact taaggctggg gctagtacca ggaccttcat gtatcaggtt
     8281 agagattttg atgcttttgg tgatttttgt gccaagcatg tactgtcacc tgggatcaat
     8341 ctattactct ttctatgaaa taattattcc aaagaggctg acagtccagg gaggagatag
     8401 cccagtggaa ggactgtcct acttgttact tatgcaaggc cagaagcacc tggttcatct
     8461 gaaggtgaag agaaaccatt ttgtgaataa ctttccagtc tacagttacc acaatggcct
     8521 cctggggcaa gaatcgcctt tcatctcaca tgactgccac tatgaaggct acatagaagg
     8581 agtgtcaggt tcttttgttt ctgtcaacat ctgtgcaggt ctcaggggca catcctcctg
     8641 attaaggagg aaaaatctta cagcattgag cccatggact cttcaagacg gtttgaacat
     8701 gtgttataca ccatggcaca tcaagcgtga gtctcctgtg gtgtctacta gctggcaaca
     8761 agggagcagg aagcctcatg atctacaggc actgtcctac ttgtggtcac acaaaaagta
     8821 cgtggagatg tttgtcgtgg tcaacaacca gcggttccag atgtggggca gtaacgtcaa
     8881 tgagacggtc cagacagtag tggatgtcat tgctctggcc aacagcttca ctaggggaat
     8941 aaacacagag gtggtgctgg ctggaatgga gatttggacc gagggggacc taatagatgt
     9001 cacagtggac ttgcaaatca cactcaggaa tttcaatcac tggagacaag agatgctctt
     9061 ccatcgtgca aaacacgatg ttgcccacat gatcgttggg catcaccctg gacagaatat
     9121 gggccaggcc tttctcagtg gtgcctgctc aagcggtttt gcggcagctg ttgaatcctt
     9181 ccatcatgaa gatgtgctgt tgtttgcagc cctgatggcc catgagctcg ggcacaacct
     9241 gggtattcag cacgaccact cggcctgctt ttgtaaagat aagcactttt gcctcatgca
     9301 tgaaaatatc acaaaagaaa gtggcttcag cagctgcagc tctgactact tctaccagtt
     9361 ccttcgagaa cacaaagggg cctgcctatt taacaagcca cggcccaggg gccgcaagcg
     9421 tagggattct gcctgtggaa atggtgtggt ggaggacacg gagcagtgtg actgtggttc
     9481 tctatgtcag catcatgcat gctgtgatga aaactgtata ctgaaggcga aagcagagtg
     9541 cagtgatggt ccatgttgtc ataagtgtaa atttcaccgt aagggatatc cttgctgtcc
     9601 ttctagtcgt tcctgtgatc tcccagaatt ttgcaatggt acatctgcat tatgccccaa
     9661 caacaggcat aagcaagatg gctcaaaatg tcatacaatt tacgagtgcc ttaaagttca
     9721 ttgtatggac cctaataatc agtgcttaca attatatgga tatggtgcaa aatcagcctc
     9781 acaagagtgt tacaattcaa tgaacagcaa aggggaccaa tttggaaact gtggcatttc
     9841 taccagtcct gggtcacaat atgttcggtg ttcagatggt aatatatttt gtgggaaact
     9901 tatatgttca ggtattacag gcttaccaaa aatcaatctc caacatacaa tgattcaggt
     9961 ccctcaggga gatggctcat gttggagcat ggatgcctat atgagtactg acattcctga
    10021 tgaaggagat gtgcacaatg gcacttactg tgcaccaaac aaagtctgcc tgaattccgc
    10081 ctgcacagat aaaaccccag tgatttctgc ctgcaaccca gaaaaaacgt gtaatgggaa
    10141 gggagtttgt aatgatttag ggcactgcca ctgtaatgaa gggcatgccc cccctgactg
    10201 tgttactgca ggaagtggag gtagtgtgga cagtggccct cctggtaagc taggtgggac
    10261 accttcagga gaaggtgaaa atcacaatat gactcattcc agacgtgaag aacatgctgt
    10321 agacatgatg atattatcat tcattatact ttttataata ttattattaa gtacaattat
    10381 ttgatctgct tgcttaaaaa atcaccagag gctgccccgg cagaagctcc tccagcagtg
    10441 gctccaccac cggccccaga aataaagcca gaagcagcag aagtggccac agaagaaaaa
    10501 gaagagaagg aggaagaaaa agaagaggag gaggaggaag aagaggagga agaatcagat
    10561 tcctaaggtt agaaataggg agatgaagcc aagtatatca aactcctcaa gtactgagtg
    10621 ggaatgagat gtttggtgaa gtagaaatag atagctttag tggctctgac tcagatatac
    10681 tgatgtagaa ggaaggattc tttcactttt attatttatt ttggtaatta aatttacatt
    10741 aatttaatat gttaaatatt ttagtacctt ttgctttttc acatttcatt tgaaccatta
    10801 acatgcatct ttggacatca atgtccttgt ctttttggcc caattgtttt agtcaccaga
    10861 aactttttca ggacatgcta tcttctattt gtttgacata cagaatcata tgtacctgaa
    10921 tttattacta actctgggat tttcatttta agtaacaact acctattatg tgaggacagt
    10981 tgtttatgtg attggttgtt ctaacttgcc ctataaatgt gcattcatca aaggagaagt
    11041 ttttaaatcc aaaaacaaat aaaacccgat tcatattaga caa
//
