LOCUS       NM_001243925            2537 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens MAPK activated protein kinase 3 (MAPKAPK3), transcript
            variant 2, mRNA.
ACCESSION   NM_001243925
VERSION     NM_001243925.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2537)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   2  (bases 1 to 2537)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   3  (bases 1 to 2537)
  AUTHORS   Buljan,M., Ciuffa,R., van Drogen,A., Vichalkovski,A., Mehnert,M.,
            Rosenberger,G., Lee,S., Varjosalo,M., Pernas,L.E., Spegg,V.,
            Snijder,B., Aebersold,R. and Gstaiger,M.
  TITLE     Kinase Interaction Network Expands Functional and Disease Roles of
            Human Kinases
  JOURNAL   Mol Cell 79 (3), 504-520 (2020)
   PUBMED   32707033
REFERENCE   4  (bases 1 to 2537)
  AUTHORS   Kennedy,S.A., Jarboui,M.A., Srihari,S., Raso,C., Bryan,K.,
            Dernayka,L., Charitou,T., Bernal-Llinares,M., Herrera-Montavez,C.,
            Krstic,A., Matallanas,D., Kotlyar,M., Jurisica,I., Curak,J.,
            Wong,V., Stagljar,I., LeBihan,T., Imrie,L., Pillai,P., Lynn,M.A.,
            Fasterius,E., Al-Khalili Szigyarto,C., Breen,J., Kiel,C.,
            Serrano,L., Rauch,N., Rukhlenko,O., Kholodenko,B.N.,
            Iglesias-Martinez,L.F., Ryan,C.J., Pilkington,R., Cammareri,P.,
            Sansom,O., Shave,S., Auer,M., Horn,N., Klose,F., Ueffing,M.,
            Boldt,K., Lynn,D.J. and Kolch,W.
  TITLE     Extensive rewiring of the EGFR network in colorectal cancer cells
            expressing transforming levels of KRASG13D
  JOURNAL   Nat Commun 11 (1), 499 (2020)
   PUBMED   31980649
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2537)
  AUTHORS   Ludwig,S., Engel,K., Hoffmeyer,A., Sithanandam,G., Neufeld,B.,
            Palm,D., Gaestel,M. and Rapp,U.R.
  TITLE     3pK, a novel mitogen-activated protein (MAP) kinase-activated
            protein kinase, is targeted by three MAP kinase pathways
  JOURNAL   Mol Cell Biol 16 (12), 6687-6697 (1996)
   PUBMED   8943323
REFERENCE   6  (bases 1 to 2537)
  AUTHORS   Clifton,A.D., Young,P.R. and Cohen,P.
  TITLE     A comparison of the substrate specificity of MAPKAP kinase-2 and
            MAPKAP kinase-3 and their activation by cytokines and cellular
            stress
  JOURNAL   FEBS Lett 392 (3), 209-214 (1996)
   PUBMED   8774846
REFERENCE   7  (bases 1 to 2537)
  AUTHORS   McLaughlin,M.M., Kumar,S., McDonnell,P.C., Van Horn,S., Lee,J.C.,
            Livi,G.P. and Young,P.R.
  TITLE     Identification of mitogen-activated protein (MAP) kinase-activated
            protein kinase-3, a novel substrate of CSBP p38 MAP kinase
  JOURNAL   J Biol Chem 271 (14), 8488-8492 (1996)
   PUBMED   8626550
REFERENCE   8  (bases 1 to 2537)
  AUTHORS   Sithanandam,G., Latif,F., Duh,F.M., Bernal,R., Smola,U., Li,H.,
            Kuzmin,I., Wixler,V., Geil,L. and Shrestha,S.
  TITLE     3pK, a new mitogen-activated protein kinase-activated protein
            kinase located in the small cell lung cancer tumor suppressor gene
            region
  JOURNAL   Mol Cell Biol 16 (3), 868-876 (1996)
   PUBMED   8622688
  REMARK    Erratum:[Mol Cell Biol 1996 Apr;16(4):1880]
REFERENCE   9  (bases 1 to 2537)
  AUTHORS   Ben-Levy,R., Leighton,I.A., Doza,Y.N., Attwood,P., Morrice,N.,
            Marshall,C.J. and Cohen,P.
  TITLE     Identification of novel phosphorylation sites required for
            activation of MAPKAP kinase-2
  JOURNAL   EMBO J 14 (23), 5920-5930 (1995)
   PUBMED   8846784
REFERENCE   10 (bases 1 to 2537)
  AUTHORS   Lavoie,J.N., Lambert,H., Hickey,E., Weber,L.A. and Landry,J.
  TITLE     Modulation of cellular thermoresistance and actin filament
            stability accompanies phosphorylation-induced changes in the
            oligomeric structure of heat shock protein 27
  JOURNAL   Mol Cell Biol 15 (1), 505-516 (1995)
   PUBMED   7799959
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BM848086.1, U09578.1,
            AC096920.2 and BE301676.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_001243925.1.
            
            Summary: This gene encodes a member of the Ser/Thr protein kinase
            family. This kinase functions as a mitogen-activated protein kinase
            (MAP kinase)- activated protein kinase. MAP kinases are also known
            as extracellular signal-regulated kinases (ERKs), act as an
            integration point for multiple biochemical signals. This kinase was
            shown to be activated by growth inducers and stress stimulation of
            cells. In vitro studies demonstrated that ERK, p38 MAP kinase and
            Jun N-terminal kinase were all able to phosphorylate and activate
            this kinase, which suggested the role of this kinase as an
            integrative element of signaling in both mitogen and stress
            responses. This kinase was reported to interact with, phosphorylate
            and repress the activity of E47, which is a basic helix-loop-helix
            transcription factor known to be involved in the regulation of
            tissue-specific gene expression and cell differentiation. Alternate
            splicing results in multiple transcript variants that encode the
            same protein. [provided by RefSeq, Sep 2011].
            
            Transcript Variant: This variant (2) differs in the 5' UTR compared
            to variant 1. Variants 1, 2 and 3 encode the same protein.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660807.211140.1,
                                           SRR1660807.98658.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1966682, SAMEA1968540
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000621469.5/ ENSP00000478922.1
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-52                BM848086.1         21-72
            53-1392             U09578.1           1-1340
            1393-2197           AC096920.2         64595-65399         c
            2198-2537           BE301676.1         12-351              c
FEATURES             Location/Qualifiers
     source          1..2537
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="3"
                     /map="3p21.2"
     gene            1..2537
                     /gene="MAPKAPK3"
                     /gene_synonym="3PK; MAPKAP-K3; MAPKAP3; MAPKAPK-3; MDPT3;
                     MK-3; MK3"
                     /note="MAPK activated protein kinase 3"
                     /db_xref="GeneID:7867"
                     /db_xref="HGNC:HGNC:6888"
                     /db_xref="MIM:602130"
     exon            1..91
                     /gene="MAPKAPK3"
                     /gene_synonym="3PK; MAPKAP-K3; MAPKAP3; MAPKAPK-3; MDPT3;
                     MK-3; MK3"
                     /inference="alignment:Splign:2.1.0"
     exon            92..362
                     /gene="MAPKAPK3"
                     /gene_synonym="3PK; MAPKAP-K3; MAPKAP3; MAPKAPK-3; MDPT3;
                     MK-3; MK3"
                     /inference="alignment:Splign:2.1.0"
     CDS             144..1292
                     /gene="MAPKAPK3"
                     /gene_synonym="3PK; MAPKAP-K3; MAPKAP3; MAPKAPK-3; MDPT3;
                     MK-3; MK3"
                     /EC_number="2.7.11.1"
                     /note="MAPKAP kinase 3; chromosome 3p kinase;
                     mitogen-activated protein kinase-activated protein kinase
                     3"
                     /codon_start=1
                     /product="MAP kinase-activated protein kinase 3"
                     /protein_id="NP_001230854.1"
                     /db_xref="CCDS:CCDS2832.1"
                     /db_xref="GeneID:7867"
                     /db_xref="HGNC:HGNC:6888"
                     /db_xref="MIM:602130"
                     /translation="MDGETAEEQGGPVPPPVAPGGPGLGGAPGGRREPKKYAVTDDYQ
                     LSKQVLGLGVNGKVLECFHRRTGQKCALKLLYDSPKARQEVDHHWQASGGPHIVCILD
                     VYENMHHGKRCLLIIMECMEGGELFSRIQERGDQAFTEREAAEIMRDIGTAIQFLHSH
                     NIAHRDVKPENLLYTSKEKDAVLKLTDFGFAKETTQNALQTPCYTPYYVAPEVLGPEK
                     YDKSCDMWSLGVIMYILLCGFPPFYSNTGQAISPGMKRRIRLGQYGFPNPEWSEVSED
                     AKQLIRLLLKTDPTERLTITQFMNHPWINQSMVVPQTPLHTARVLQEDKDHWDEVKEE
                     MTSALATMRVDYDQVKIKDLKTSNNRLLNKRRKKQAGSSSASQGCNNQ"
     exon            363..502
                     /gene="MAPKAPK3"
                     /gene_synonym="3PK; MAPKAP-K3; MAPKAP3; MAPKAPK-3; MDPT3;
                     MK-3; MK3"
                     /inference="alignment:Splign:2.1.0"
     exon            503..567
                     /gene="MAPKAPK3"
                     /gene_synonym="3PK; MAPKAP-K3; MAPKAP3; MAPKAPK-3; MDPT3;
                     MK-3; MK3"
                     /inference="alignment:Splign:2.1.0"
     exon            568..647
                     /gene="MAPKAPK3"
                     /gene_synonym="3PK; MAPKAP-K3; MAPKAP3; MAPKAPK-3; MDPT3;
                     MK-3; MK3"
                     /inference="alignment:Splign:2.1.0"
     exon            648..771
                     /gene="MAPKAPK3"
                     /gene_synonym="3PK; MAPKAP-K3; MAPKAP3; MAPKAPK-3; MDPT3;
                     MK-3; MK3"
                     /inference="alignment:Splign:2.1.0"
     exon            772..847
                     /gene="MAPKAPK3"
                     /gene_synonym="3PK; MAPKAP-K3; MAPKAP3; MAPKAPK-3; MDPT3;
                     MK-3; MK3"
                     /inference="alignment:Splign:2.1.0"
     exon            848..972
                     /gene="MAPKAPK3"
                     /gene_synonym="3PK; MAPKAP-K3; MAPKAP3; MAPKAPK-3; MDPT3;
                     MK-3; MK3"
                     /inference="alignment:Splign:2.1.0"
     exon            973..1058
                     /gene="MAPKAPK3"
                     /gene_synonym="3PK; MAPKAP-K3; MAPKAP3; MAPKAPK-3; MDPT3;
                     MK-3; MK3"
                     /inference="alignment:Splign:2.1.0"
     exon            1059..1139
                     /gene="MAPKAPK3"
                     /gene_synonym="3PK; MAPKAP-K3; MAPKAP3; MAPKAPK-3; MDPT3;
                     MK-3; MK3"
                     /inference="alignment:Splign:2.1.0"
     exon            1140..2537
                     /gene="MAPKAPK3"
                     /gene_synonym="3PK; MAPKAP-K3; MAPKAP3; MAPKAPK-3; MDPT3;
                     MK-3; MK3"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2514..2519
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MAPKAPK3"
                     /gene_synonym="3PK; MAPKAP-K3; MAPKAP3; MAPKAPK-3; MDPT3;
                     MK-3; MK3"
                     /note="hexamer: AATAAA"
     polyA_site      2537
                     /gene="MAPKAPK3"
                     /gene_synonym="3PK; MAPKAP-K3; MAPKAP3; MAPKAPK-3; MDPT3;
                     MK-3; MK3"
                     /note="major polyA site"
ORIGIN      
        1 actctcggcc ctgggatttc tgcggccgcc agctcccgcg accgcctctc ctgcccctcg
       61 ccggtacctc agcaaggtgc gttgccgcca ggtgccacta gaagcgccag gctggggccg
      121 cctctgagcg ccccgcgggg gccatggatg gtgaaacagc agaggagcag gggggccctg
      181 tgcccccgcc agttgcaccc ggcggacccg gcttgggcgg tgctccgggg gggcggcggg
      241 agcccaagaa gtacgcagtg accgacgact accagttgtc caagcaggtg ctgggcctgg
      301 gtgtgaacgg caaagtgctg gagtgcttcc atcggcgcac tggacagaag tgtgccctga
      361 agctcctgta tgacagcccc aaggcccggc aggaggtaga ccatcactgg caggcttctg
      421 gcggccccca tattgtctgc atcctggatg tgtatgagaa catgcaccat ggcaagcgct
      481 gtctcctcat catcatggaa tgcatggaag gtggtgagtt gttcagcagg attcaggagc
      541 gtggcgacca ggctttcact gagagagaag ctgcagagat aatgcgggat attggcactg
      601 ccatccagtt tctgcacagc cataacattg cccaccgaga tgtcaagcct gaaaacctac
      661 tctacacatc taaggagaaa gacgcagtgc ttaagctcac cgattttggc tttgctaagg
      721 agaccaccca aaatgccctg cagacaccct gctatactcc ctattatgtg gcccctgagg
      781 tcctgggtcc agagaagtat gacaagtcat gtgacatgtg gtccctgggt gtcatcatgt
      841 acatcctcct ttgtggcttc ccacccttct actccaacac gggccaggcc atctccccgg
      901 ggatgaagag gaggattcgc ctgggccagt acggcttccc caatcctgag tggtcagaag
      961 tctctgagga tgccaagcag ctgatccgcc tcctgttgaa gacagacccc acagagaggc
     1021 tgaccatcac tcagttcatg aaccacccct ggatcaacca atcgatggta gtgccacaga
     1081 ccccactcca cacggcccga gtgctgcagg aggacaaaga ccactgggac gaagtcaagg
     1141 aggagatgac cagtgccttg gccactatgc gggtagacta cgaccaggtg aagatcaagg
     1201 acctgaagac ctctaacaac cggctcctca acaagaggag aaaaaagcag gcaggcagct
     1261 cctctgcctc acagggctgc aacaaccagt agctcatggg gccttggagg agcctggcct
     1321 ctcagcctgc ataacagact gaaatgtgct caggccctgg ccaggagggc ccagggtcat
     1381 tcttttaaca aaaggattat tttgttgtgt tttaatttgt cactcggaac ttcaggatgg
     1441 aggaccctga ccctaaacct ccttcagatc tctggcccag gctcaagccc tagagatggg
     1501 cagggcctag gggctgggag ctgcctgctg ccatagcagc acctttagct aggttggccc
     1561 gagtgaggcc tctgtgctgt cctgccctgg tgcatggcct tagctttcta ggccactggg
     1621 agttgtggct gggcttccca tcttccacag agacatctcc ctgtgggatg ggcagatggg
     1681 cctggccttg agaaaggcat tggccattgg ttgccatggt gaccagggac cacgttgctg
     1741 cctgtgaatg ctgagtgagc gagtaaggga ggaggggcga ttgagggttc acctctgcct
     1801 tggggaggct gatttctcac acactggctg gccctctcat tctcactcct ccttgggccc
     1861 tgaggctgct ggatccggtc tgcctgcctc cctgtgcagt ccagccctgc cttgctgcag
     1921 ccccagccca gatggcactc agcgctctcc cctgagggag tccctgggcc tagccatccc
     1981 ctcactattc ccgacccaaa gggtgacttt tcatctgaac ttaaggtggg agatattttt
     2041 aacttttttc cactttggaa aatgtcactg tgacaaaagc cagcatactt tccctgcacc
     2101 catctgctca ccagatctca ggcaggaaag cccctctctg ttgaagtcag gggctatctt
     2161 ttggtatact tgtgtgaaag tggctggttg ggagcagagc taagtggctt cccattaacc
     2221 tgaggtctct ttctttactc tgggtcagac ctgaggttgg ggaaggcgac tgagccatgc
     2281 tcagaatgtc tggtcctggc ttgggcctga gtagggcaga gagggccttt cctggctgat
     2341 cagagcttac cagccccacc ccaccatggt agccttaggg tgctgagtgc ctgatactgc
     2401 ctgacaagtg cctgacacgc agcctagttc cttcctggcc cctctctcac tggctgggaa
     2461 accctagacc atgtcagata ggacaacact gctgggtttt acatccagat agtaataaac
     2521 accatttcat cattttc
//
