LOCUS       NM_001006617            3369 bp    mRNA    linear   PRI 12-FEB-2025
DEFINITION  Homo sapiens MAPK associated protein 1 (MAPKAP1), transcript
            variant 1, mRNA.
ACCESSION   NM_001006617
VERSION     NM_001006617.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3369)
  AUTHORS   Ke,B., Huang,Y., Gong,Y., Zhong,H. and Shi,L.
  TITLE     Overexpression of microRNA-611 inhibits TGF-beta-induced
            epithelial-mesenchymal transition and migration in lung cancer
            cells through MAPKAP1
  JOURNAL   Cell Signal 123, 111357 (2024)
   PUBMED   39173858
  REMARK    GeneRIF: Overexpression of microRNA-611 inhibits TGF-beta-induced
            epithelial-mesenchymal transition and migration in lung cancer
            cells through MAPKAP1.
REFERENCE   2  (bases 1 to 3369)
  AUTHORS   Ezine,E., Lebbe,C. and Dumaz,N.
  TITLE     Unmasking the tumourigenic role of SIN1/MAPKAP1 in the mTOR complex
            2
  JOURNAL   Clin Transl Med 13 (10), e1464 (2023)
   PUBMED   37877351
  REMARK    GeneRIF: Unmasking the tumourigenic role of SIN1/MAPKAP1 in the
            mTOR complex 2.
            Review article
REFERENCE   3  (bases 1 to 3369)
  AUTHORS   Yu,Z., Chen,J., Takagi,E., Wang,F., Saha,B., Liu,X., Joubert,L.M.,
            Gleason,C.E., Jin,M., Li,C., Nowotny,C., Agard,D., Cheng,Y. and
            Pearce,D.
  TITLE     Interactions between mTORC2 core subunits Rictor and mSin1 dictate
            selective and context-dependent phosphorylation of substrate
            kinases SGK1 and Akt
  JOURNAL   J Biol Chem 298 (9), 102288 (2022)
   PUBMED   35926713
  REMARK    GeneRIF: Interactions between mTORC2 core subunits Rictor and mSin1
            dictate selective and context-dependent phosphorylation of
            substrate kinases SGK1 and Akt.
REFERENCE   4  (bases 1 to 3369)
  AUTHORS   Castel,P., Dharmaiah,S., Sale,M.J., Messing,S., Rizzuto,G.,
            Cuevas-Navarro,A., Cheng,A., Trnka,M.J., Urisman,A., Esposito,D.,
            Simanshu,D.K. and McCormick,F.
  TITLE     RAS interaction with Sin1 is dispensable for mTORC2 assembly and
            activity
  JOURNAL   Proc Natl Acad Sci U S A 118 (33) (2021)
   PUBMED   34380736
  REMARK    GeneRIF: RAS interaction with Sin1 is dispensable for mTORC2
            assembly and activity.
REFERENCE   5  (bases 1 to 3369)
  AUTHORS   Wrobel,L., Siddiqi,F.H., Hill,S.M., Son,S.M., Karabiyik,C., Kim,H.
            and Rubinsztein,D.C.
  TITLE     mTORC2 Assembly Is Regulated by USP9X-Mediated Deubiquitination of
            RICTOR
  JOURNAL   Cell Rep 33 (13), 108564 (2020)
   PUBMED   33378666
REFERENCE   6  (bases 1 to 3369)
  AUTHORS   Sarbassov,D.D., Guertin,D.A., Ali,S.M. and Sabatini,D.M.
  TITLE     Phosphorylation and regulation of Akt/PKB by the rictor-mTOR
            complex
  JOURNAL   Science 307 (5712), 1098-1101 (2005)
   PUBMED   15718470
REFERENCE   7  (bases 1 to 3369)
  AUTHORS   Schroder,W., Cloonan,N., Bushell,G. and Sculley,T.
  TITLE     Alternative polyadenylation and splicing of mRNAs transcribed from
            the human Sin1 gene
  JOURNAL   Gene 339, 17-23 (2004)
   PUBMED   15363842
  REMARK    GeneRIF: Results demonstrate that Sin1 transcripts can use
            alternative polyadenylation signals and describe a number of Sin1
            splice variants that potentially encode functionally different
            isoforms.
REFERENCE   8  (bases 1 to 3369)
  AUTHORS   Jin,J., Smith,F.D., Stark,C., Wells,C.D., Fawcett,J.P.,
            Kulkarni,S., Metalnikov,P., O'Donnell,P., Taylor,P., Taylor,L.,
            Zougman,A., Woodgett,J.R., Langeberg,L.K., Scott,J.D. and Pawson,T.
  TITLE     Proteomic, functional, and domain-based analysis of in vivo 14-3-3
            binding proteins involved in cytoskeletal regulation and cellular
            organization
  JOURNAL   Curr Biol 14 (16), 1436-1450 (2004)
   PUBMED   15324660
REFERENCE   9  (bases 1 to 3369)
  AUTHORS   Sarbassov,D.D., Ali,S.M., Kim,D.H., Guertin,D.A., Latek,R.R.,
            Erdjument-Bromage,H., Tempst,P. and Sabatini,D.M.
  TITLE     Rictor, a novel binding partner of mTOR, defines a
            rapamycin-insensitive and raptor-independent pathway that regulates
            the cytoskeleton
  JOURNAL   Curr Biol 14 (14), 1296-1302 (2004)
   PUBMED   15268862
REFERENCE   10 (bases 1 to 3369)
  AUTHORS   Colicelli,J., Nicolette,C., Birchmeier,C., Rodgers,L., Riggs,M. and
            Wigler,M.
  TITLE     Expression of three mammalian cDNAs that interfere with RAS
            function in Saccharomyces cerevisiae
  JOURNAL   Proc Natl Acad Sci U S A 88 (7), 2913-2917 (1991)
   PUBMED   1849280
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL358074.22, AL162584.9 and
            AL359632.9.
            
            On Aug 31, 2019 this sequence version replaced NM_001006617.2.
            
            Summary: This gene encodes a protein that is highly similar to the
            yeast SIN1 protein, a stress-activated protein kinase.
            Alternatively spliced transcript variants encoding distinct
            isoforms have been described. Alternate polyadenylation sites as
            well as alternate 3' UTRs have been identified for transcripts of
            this gene. [provided by RefSeq, Jul 2008].
            
            Transcript Variant:  This variant (1), also known as Sin1 and
            Mip1b, represents the longest transcript and encodes the longest
            isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AY524429.1, SRR11853559.32578.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000265960.8/ ENSP00000265960.3
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-238               AL358074.22        25385-25622         c
            239-566             AL162584.9         148909-149236       c
            567-656             AL162584.9         146411-146500       c
            657-805             AL162584.9         134244-134392       c
            806-978             AL162584.9         62148-62320         c
            979-1155            AL162584.9         36226-36402         c
            1156-1265           AL162584.9         19652-19761         c
            1266-1373           AL359632.9         76300-76407         c
            1374-1514           AL359632.9         54433-54573         c
            1515-1652           AL359632.9         37962-38099         c
            1653-1750           AL359632.9         14491-14588         c
            1751-3369           AL359632.9         7384-9002           c
FEATURES             Location/Qualifiers
     source          1..3369
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="9"
                     /map="9q33.3"
     gene            1..3369
                     /gene="MAPKAP1"
                     /gene_synonym="JC310; MIP1; SIN1; SIN1b; SIN1g"
                     /note="MAPK associated protein 1"
                     /db_xref="GeneID:79109"
                     /db_xref="HGNC:HGNC:18752"
                     /db_xref="MIM:610558"
     exon            1..238
                     /gene="MAPKAP1"
                     /gene_synonym="JC310; MIP1; SIN1; SIN1b; SIN1g"
                     /inference="alignment:Splign:2.1.0"
     exon            239..566
                     /gene="MAPKAP1"
                     /gene_synonym="JC310; MIP1; SIN1; SIN1b; SIN1g"
                     /inference="alignment:Splign:2.1.0"
     CDS             308..1876
                     /gene="MAPKAP1"
                     /gene_synonym="JC310; MIP1; SIN1; SIN1b; SIN1g"
                     /note="isoform 1 is encoded by transcript variant 1; ras
                     inhibitor MGC2745; stress-activated protein
                     kinase-interacting 1; stress-activated map kinase
                     interacting protein 1; mitogen-activated protein kinase
                     associated protein 1; target of rapamycin complex 2
                     subunit MAPKAP1; MEKK2-interacting protein 1;
                     SAPK-interacting protein 1; mSIN1; TORC2 subunit MAPKAP1;
                     mitogen-activated protein kinase 2-associated protein 1"
                     /codon_start=1
                     /product="target of rapamycin complex 2 subunit MAPKAP1
                     isoform 1"
                     /protein_id="NP_001006618.1"
                     /db_xref="CCDS:CCDS35140.1"
                     /db_xref="GeneID:79109"
                     /db_xref="HGNC:HGNC:18752"
                     /db_xref="MIM:610558"
                     /translation="MAFLDNPTIILAHIRQSHVTSDDTGMCEMVLIDHDVDLEKIHPP
                     SMPGDSGSEIQGSNGETQGYVYAQSVDITSSWDFGIRRRSNTAQRLERLRKERQNQIK
                     CKNIQWKERNSKQSAQELKSLFEKKSLKEKPPISGKQSILSVRLEQCPLQLNNPFNEY
                     SKFDGKGHVGTTATKKIDVYLPLHSSQDRLLPMTVVTMASARVQDLIGLICWQYTSEG
                     REPKLNDNVSAYCLHIAEDDGEVDTDFPPLDSNEPIHKFGFSTLALVEKYSSPGLTSK
                     ESLFVRINAAHGFSLIQVDNTKVTMKEILLKAVKRRKGSQKVSGPQYRLEKQSEPNVA
                     VDLDSTLESQSAWEFCLVRENSSRADGVFEEDSQIDIATVQDMLSSHHYKSFKVSMIH
                     RLRFTTDVQLGISGDKVEIDPVTNQKASTKFWIKQKPISIDSDLLCACDLAEEKSPSH
                     AIFKLTYLSNHDYKHLYFESDAATVNEIVLKVNYILESRASTARADYFAQKQRKLNRR
                     TSFSFQKEKKSGQQ"
     exon            567..656
                     /gene="MAPKAP1"
                     /gene_synonym="JC310; MIP1; SIN1; SIN1b; SIN1g"
                     /inference="alignment:Splign:2.1.0"
     exon            657..805
                     /gene="MAPKAP1"
                     /gene_synonym="JC310; MIP1; SIN1; SIN1b; SIN1g"
                     /inference="alignment:Splign:2.1.0"
     exon            806..978
                     /gene="MAPKAP1"
                     /gene_synonym="JC310; MIP1; SIN1; SIN1b; SIN1g"
                     /inference="alignment:Splign:2.1.0"
     exon            979..1155
                     /gene="MAPKAP1"
                     /gene_synonym="JC310; MIP1; SIN1; SIN1b; SIN1g"
                     /inference="alignment:Splign:2.1.0"
     exon            1156..1265
                     /gene="MAPKAP1"
                     /gene_synonym="JC310; MIP1; SIN1; SIN1b; SIN1g"
                     /inference="alignment:Splign:2.1.0"
     exon            1266..1373
                     /gene="MAPKAP1"
                     /gene_synonym="JC310; MIP1; SIN1; SIN1b; SIN1g"
                     /inference="alignment:Splign:2.1.0"
     exon            1374..1514
                     /gene="MAPKAP1"
                     /gene_synonym="JC310; MIP1; SIN1; SIN1b; SIN1g"
                     /inference="alignment:Splign:2.1.0"
     exon            1515..1652
                     /gene="MAPKAP1"
                     /gene_synonym="JC310; MIP1; SIN1; SIN1b; SIN1g"
                     /inference="alignment:Splign:2.1.0"
     exon            1653..1750
                     /gene="MAPKAP1"
                     /gene_synonym="JC310; MIP1; SIN1; SIN1b; SIN1g"
                     /inference="alignment:Splign:2.1.0"
     exon            1751..3369
                     /gene="MAPKAP1"
                     /gene_synonym="JC310; MIP1; SIN1; SIN1b; SIN1g"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2271..2276
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MAPKAP1"
                     /gene_synonym="JC310; MIP1; SIN1; SIN1b; SIN1g"
                     /note="hexamer: AATAAA"
     polyA_site      2291
                     /gene="MAPKAP1"
                     /gene_synonym="JC310; MIP1; SIN1; SIN1b; SIN1g"
                     /note="major polyA site"
     regulatory      3347..3352
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MAPKAP1"
                     /gene_synonym="JC310; MIP1; SIN1; SIN1b; SIN1g"
                     /note="hexamer: AATAAA"
     polyA_site      3369
                     /gene="MAPKAP1"
                     /gene_synonym="JC310; MIP1; SIN1; SIN1b; SIN1g"
ORIGIN      
        1 gtcgtgtgcg gctcggggta atagggctgc tgctcggccg gccggcggcg gcgagcagca
       61 ggggcatgag ggctaacccg ggaagcggca gctgagcggg ccgggaggag cgccggtccc
      121 cgtggatccc gagagtgcag agctcggggc aggggccggg aggcgtgggg gagccgggcc
      181 ctcccctcag gaacgtgtcc cggggccgac ccggcccgta gtgtggaagc agcttcaggt
      241 aggtgagctc gtgaaacaat atgaagagga gaaaatagcc ttttaaggaa attggcccac
      301 agaaaggatg gccttcttgg acaatccaac tatcattcta gctcatattc gacagtcaca
      361 tgtgaccagt gatgacacgg gaatgtgtga gatggttctc attgatcatg atgttgacct
      421 agagaagatt catcctcctt caatgcctgg agacagtggg tcagaaattc agggaagcaa
      481 tggtgagact cagggctatg tatatgccca gtcagtcgat attacctcaa gttgggactt
      541 tggtattaga agacgctcaa acacagctca aagattagaa cgactccgaa aagagagaca
      601 aaaccagatc aaatgcaaaa atattcagtg gaaagaaaga aattctaagc aatcagccca
      661 ggagttaaag tcactgtttg aaaaaaaatc tctcaaagag aagcctccaa tttctgggaa
      721 gcagtcgata ttatctgtac gcctagaaca gtgccctctg cagctgaata acccttttaa
      781 cgagtattcc aaatttgatg gcaagggtca tgtaggtaca acagcaacca agaagatcga
      841 tgtctacctc cctctgcact cgagccagga cagactgctg ccaatgaccg tggtgacaat
      901 ggccagcgcc agggtgcagg acctgatcgg gctcatctgc tggcagtata caagcgaagg
      961 acgggagccg aagctcaatg acaatgtcag tgcctactgc ctgcatattg ctgaggatga
     1021 tggggaggtg gacaccgatt tccccccgct ggattccaat gagcccattc ataagtttgg
     1081 cttcagtact ttggccctgg ttgaaaagta ctcatctcct ggtctgacat ccaaagagtc
     1141 actctttgtt cgaataaatg ctgctcatgg attctccctt attcaggtgg acaacacaaa
     1201 ggttaccatg aaggaaatct tactgaaggc agtgaagcga agaaaaggat cccagaaagt
     1261 ttcaggccct cagtaccgcc tggagaagca gagcgagccc aatgtcgccg ttgacctgga
     1321 cagcactttg gagagccaga gcgcatggga gttctgcctg gtccgcgaga acagttcaag
     1381 ggcagacggg gtttttgagg aggattcgca aattgacata gccacagtac aggatatgct
     1441 tagcagccac cattacaagt cattcaaagt cagcatgatc cacagactgc gattcacaac
     1501 cgacgtacag ctaggtatct ctggagacaa agtagagata gaccctgtta cgaatcagaa
     1561 agccagcact aagttttgga ttaagcagaa acccatctca atcgattccg acctgctctg
     1621 tgcctgtgac cttgctgaag agaaaagccc cagtcacgca atatttaaac tcacgtatct
     1681 aagcaatcac gactataaac acctctactt tgaatcggac gctgctaccg tcaatgaaat
     1741 tgtgctcaag gttaactaca tcctggaatc gcgagctagc actgcccggg ctgactactt
     1801 tgctcaaaaa caaagaaaac tgaacagacg tacgagcttc agcttccaga aggagaagaa
     1861 atccgggcag cagtgacact ggcctccagc ctcaatctgt tccgtagctc agagcctgcc
     1921 tgccagggcc aagtgcccta gagtccaccc ggtgtcctga agtcctcggg gggaggccag
     1981 cccctggctc actggcacag ggcaggtggg ctctcgggga aggtgtcggg ggccccctag
     2041 gagggagcgc tggggacatt gccatgggac ggaagtctgc ttggcagtgg ctttgataag
     2101 cgatgcttgg gggtcagacc accccctaga ggagccacgt gccgcccagc caccttcaat
     2161 gcctgccacc ctgcccgagg atgtacagag ccgtgcccac acatttcctt gcaacttgat
     2221 caaatttctt aaagcaaaca acaaaaatgt acatttctgt ttttcctttt aataaacagg
     2281 tgtactcttt atcatggttg gtatgatgga ccattctttg gggcggagga ttgattatgt
     2341 tactctcttt aaaatctgtt cccatattga acaggcagat tggaaaagct atggttcgat
     2401 ttctcagaag aaatgtttag gtcttagtca atagttttaa ctatgccatt tgtttaaatg
     2461 agtgcatttg cttcgagggt agtgtcttac taaaagttag gaacagagac ctagtggtgt
     2521 gtccaaggcc gtgtcacttt ccccttcagc acaccccagc ttctgacctc agagcccagg
     2581 agctgcgtgg acagtgtggg gtgccaggag gaggggcggt ggctggtcct caggcacgct
     2641 gcactcccag ccagacatgg tctttccgtt tcttaagtag caagtgtagg tttcagctgg
     2701 cagttccacc tgcatgttct ctgcttcgct gccttggaag gggccacatt ccccattcct
     2761 cttctcctta cagcgcctgc ctcctttttc aagcaggcgg aaagctgctg tttctcacgt
     2821 ttcagggaga ggggtgagcg gagggagacc tgtgtccgtg ccgtccggct ccctgggtgg
     2881 gaacaggcaa gggatcagat gcccctgaca ccacgcctct ggccacacca gatgcctctg
     2941 cagtcctcga cagcctcttc agtgtccctc ctgcggtgat gtccttactg tccccagcca
     3001 gggccgggga ccggtgtttc actgaggacc tgcattagaa acatttttta aattgttgta
     3061 caggaagaga tgtgtctaaa acagcatctt aaagctgagt gtatttcttt gcacaagggg
     3121 tcatgctgat gaattcttct ttcattctga tctttgttca gccaacagga gcgtcctttt
     3181 ctaatgtctt ccattcctac cccccaccca aaaacaaaag aaatatttgt agcttgctat
     3241 ctgtatttga atttttagca attttatatt tagatacttt gaaaaatgta aatgactaat
     3301 ttggtcatta aatcttgtga catattcgat attaaaatga tattaaaata aaagtcatat
     3361 aaatacaca
//
