LOCUS       NM_001323329            5809 bp    mRNA    linear   PRI 26-FEB-2025
DEFINITION  Homo sapiens mitogen-activated protein kinase 8 (MAPK8), transcript
            variant 15, mRNA.
ACCESSION   NM_001323329
VERSION     NM_001323329.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5809)
  AUTHORS   Liu,X., Ye,Z., Rao,D., Chen,Q. and Zhang,Z.
  TITLE     DUSP4 maintains the survival and LSD1 protein stability in
            esophageal squamous cell carcinoma cells by inhibiting JNK
            signaling-dependent autophagy
  JOURNAL   In Vitro Cell Dev Biol Anim 60 (2), 115-122 (2024)
   PUBMED   38286920
  REMARK    GeneRIF: DUSP4 maintains the survival and LSD1 protein stability in
            esophageal squamous cell carcinoma cells by inhibiting JNK
            signaling-dependent autophagy.
REFERENCE   2  (bases 1 to 5809)
  AUTHORS   Louw-du Toit,R., Simons,M. and Africander,D.
  TITLE     Progestins and breast cancer hallmarks: The role of the ERK1/2 and
            JNK pathways in estrogen receptor positive breast cancer cells
  JOURNAL   J Steroid Biochem Mol Biol 237, 106440 (2024)
   PUBMED   38048919
  REMARK    GeneRIF: Progestins and breast cancer hallmarks: The role of the
            ERK1/2 and JNK pathways in estrogen receptor positive breast cancer
            cells.
REFERENCE   3  (bases 1 to 5809)
  AUTHORS   Chai,F., Peng,H., Qin,L., Liu,C., Zeng,Y., Wang,R., Xu,G., Wang,R.,
            Wei,G., Huang,H., Lan,Y., Chen,W. and Wang,C.
  TITLE     MicroRNA miR-181d-5p regulates the MAPK signaling pathway by
            targeting mitogen-activated protein kinase 8 (MAPK8) to improve
            lupus nephritis
  JOURNAL   Gene 893, 147961 (2024)
   PUBMED   37931853
  REMARK    GeneRIF: MicroRNA miR-181d-5p regulates the MAPK signaling pathway
            by targeting mitogen-activated protein kinase 8 (MAPK8) to improve
            lupus nephritis.
REFERENCE   4  (bases 1 to 5809)
  AUTHORS   Feng,Y., Cao,H., Wang,D., Chen,L., Gao,R. and Sun,P.
  TITLE     JNK promotes the progression of castration-resistant prostate
            cancer
  JOURNAL   Acta Biochim Pol 70 (4), 817-822 (2023)
   PUBMED   38099475
  REMARK    GeneRIF: JNK promotes the progression of castration-resistant
            prostate cancer.
REFERENCE   5  (bases 1 to 5809)
  AUTHORS   Ding,S., Wang,H., Liao,Y., Chen,R., Hu,Y., Wu,H., Shen,H. and
            Tang,S.
  TITLE     HPV16 E7 protein antagonizes TNF-alpha-induced apoptosis of
            cervical cancer cells via Daxx/JNK pathway
  JOURNAL   Microb Pathog 185, 106423 (2023)
   PUBMED   37871853
  REMARK    GeneRIF: HPV16 E7 protein antagonizes TNF-alpha-induced apoptosis
            of cervical cancer cells via Daxx/JNK pathway.
REFERENCE   6  (bases 1 to 5809)
  AUTHORS   Kharbanda,S., Saleem,A., Shafman,T., Emoto,Y., Taneja,N., Rubin,E.,
            Weichselbaum,R., Woodgett,J., Avruch,J., Kyriakis,J. et al.
  TITLE     Ionizing radiation stimulates a Grb2-mediated association of the
            stress-activated protein kinase with phosphatidylinositol 3-kinase
  JOURNAL   J Biol Chem 270 (32), 18871-18874 (1995)
   PUBMED   7642542
REFERENCE   7  (bases 1 to 5809)
  AUTHORS   van Dam,H., Wilhelm,D., Herr,I., Steffen,A., Herrlich,P. and
            Angel,P.
  TITLE     ATF-2 is preferentially activated by stress-activated protein
            kinases to mediate c-jun induction in response to genotoxic agents
  JOURNAL   EMBO J 14 (8), 1798-1811 (1995)
   PUBMED   7737130
REFERENCE   8  (bases 1 to 5809)
  AUTHORS   Livingstone,C., Patel,G. and Jones,N.
  TITLE     ATF-2 contains a phosphorylation-dependent transcriptional
            activation domain
  JOURNAL   EMBO J 14 (8), 1785-1797 (1995)
   PUBMED   7737129
REFERENCE   9  (bases 1 to 5809)
  AUTHORS   Derijard,B., Raingeaud,J., Barrett,T., Wu,I.H., Han,J.,
            Ulevitch,R.J. and Davis,R.J.
  TITLE     Independent human MAP-kinase signal transduction pathways defined
            by MEK and MKK isoforms
  JOURNAL   Science 267 (5198), 682-685 (1995)
   PUBMED   7839144
  REMARK    Erratum:[Science 1995 Jul 7;269(5220):17]
REFERENCE   10 (bases 1 to 5809)
  AUTHORS   Seth,A., Alvarez,E., Gupta,S. and Davis,R.J.
  TITLE     A phosphorylation site located in the NH2-terminal domain of c-Myc
            increases transactivation of gene expression
  JOURNAL   J Biol Chem 266 (35), 23521-23524 (1991)
   PUBMED   1748630
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC074325.7 and AC016397.6.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Jun 2, 2019 this sequence version replaced NM_001323329.1.
            
            Summary: The protein encoded by this gene is a member of the MAP
            kinase family. MAP kinases act as an integration point for multiple
            biochemical signals, and are involved in a wide variety of cellular
            processes such as proliferation, differentiation, transcription
            regulation and development. This kinase is activated by various
            cell stimuli, and targets specific transcription factors, and thus
            mediates immediate-early gene expression in response to cell
            stimuli. The activation of this kinase by tumor-necrosis factor
            alpha (TNF-alpha) is found to be required for TNF-alpha induced
            apoptosis. This kinase is also involved in UV radiation induced
            apoptosis, which is thought to be related to cytochrom c-mediated
            cell death pathway. Studies of the mouse counterpart of this gene
            suggested that this kinase play a key role in T cell proliferation,
            apoptosis and differentiation. Several alternatively spliced
            transcript variants encoding distinct isoforms have been reported.
            [provided by RefSeq, Apr 2016].
            
            Transcript Variant: This variant (15), along with variants JNK1-a2,
            14, and 17, encodes isoform alpha2.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803612.177734.1,
                                           SRR1660809.69044.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000374189.6/ ENSP00000363304.1
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-145               AC074325.7         65188-65332
            146-316             AC016397.6         49420-49590
            317-446             AC016397.6         52660-52789
            447-505             AC016397.6         57687-57745
            506-644             AC016397.6         57838-57976
            645-810             AC016397.6         67963-68128
            811-882             AC016397.6         71896-71967
            883-1065            AC016397.6         73696-73878
            1066-1190           AC016397.6         74188-74312
            1191-1254           AC016397.6         74888-74951
            1255-1332           AC016397.6         79001-79078
            1333-5809           AC016397.6         82692-87168
FEATURES             Location/Qualifiers
     source          1..5809
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="10"
                     /map="10q11.22"
     gene            1..5809
                     /gene="MAPK8"
                     /gene_synonym="JNK; JNK-46; JNK1; JNK1A2; JNK21B1/2;
                     PRKM8; SAPK1; SAPK1c"
                     /note="mitogen-activated protein kinase 8"
                     /db_xref="GeneID:5599"
                     /db_xref="HGNC:HGNC:6881"
                     /db_xref="MIM:601158"
     exon            1..145
                     /gene="MAPK8"
                     /gene_synonym="JNK; JNK-46; JNK1; JNK1A2; JNK21B1/2;
                     PRKM8; SAPK1; SAPK1c"
                     /inference="alignment:Splign:2.1.0"
     exon            146..316
                     /gene="MAPK8"
                     /gene_synonym="JNK; JNK-46; JNK1; JNK1A2; JNK21B1/2;
                     PRKM8; SAPK1; SAPK1c"
                     /inference="alignment:Splign:2.1.0"
     CDS             195..1478
                     /gene="MAPK8"
                     /gene_synonym="JNK; JNK-46; JNK1; JNK1A2; JNK21B1/2;
                     PRKM8; SAPK1; SAPK1c"
                     /EC_number="2.7.11.24"
                     /note="isoform alpha2 is encoded by transcript variant 15;
                     JUN N-terminal kinase; c-Jun N-terminal kinase 1; MAP
                     kinase 8; stress-activated protein kinase 1;
                     stress-activated protein kinase 1c"
                     /codon_start=1
                     /product="mitogen-activated protein kinase 8 isoform
                     alpha2"
                     /protein_id="NP_001310258.1"
                     /db_xref="CCDS:CCDS7224.1"
                     /db_xref="GeneID:5599"
                     /db_xref="HGNC:HGNC:6881"
                     /db_xref="MIM:601158"
                     /translation="MSRSKRDNNFYSVEIGDSTFTVLKRYQNLKPIGSGAQGIVCAAY
                     DAILERNVAIKKLSRPFQNQTHAKRAYRELVLMKCVNHKNIIGLLNVFTPQKSLEEFQ
                     DVYIVMELMDANLCQVIQMELDHERMSYLLYQMLCGIKHLHSAGIIHRDLKPSNIVVK
                     SDCTLKILDFGLARTAGTSFMMTPYVVTRYYRAPEVILGMGYKENVDLWSVGCIMGEM
                     VCHKILFPGRDYIDQWNKVIEQLGTPCPEFMKKLQPTVRTYVENRPKYAGYSFEKLFP
                     DVLFPADSEHNKLKASQARDLLSKMLVIDASKRISVDEALQHPYINVWYDPSEAEAPP
                     PKIPDKQLDEREHTIEEWKELIYKEVMDLEERTKNGVIRGQPSPLGAAVINGSQHPSS
                     SSSVNDVSSMSTDPTLASDTDSSLEAAAGPLGCCR"
     exon            317..446
                     /gene="MAPK8"
                     /gene_synonym="JNK; JNK-46; JNK1; JNK1A2; JNK21B1/2;
                     PRKM8; SAPK1; SAPK1c"
                     /inference="alignment:Splign:2.1.0"
     exon            447..505
                     /gene="MAPK8"
                     /gene_synonym="JNK; JNK-46; JNK1; JNK1A2; JNK21B1/2;
                     PRKM8; SAPK1; SAPK1c"
                     /inference="alignment:Splign:2.1.0"
     exon            506..644
                     /gene="MAPK8"
                     /gene_synonym="JNK; JNK-46; JNK1; JNK1A2; JNK21B1/2;
                     PRKM8; SAPK1; SAPK1c"
                     /inference="alignment:Splign:2.1.0"
     exon            645..810
                     /gene="MAPK8"
                     /gene_synonym="JNK; JNK-46; JNK1; JNK1A2; JNK21B1/2;
                     PRKM8; SAPK1; SAPK1c"
                     /inference="alignment:Splign:2.1.0"
     exon            811..882
                     /gene="MAPK8"
                     /gene_synonym="JNK; JNK-46; JNK1; JNK1A2; JNK21B1/2;
                     PRKM8; SAPK1; SAPK1c"
                     /inference="alignment:Splign:2.1.0"
     exon            883..1065
                     /gene="MAPK8"
                     /gene_synonym="JNK; JNK-46; JNK1; JNK1A2; JNK21B1/2;
                     PRKM8; SAPK1; SAPK1c"
                     /inference="alignment:Splign:2.1.0"
     exon            1066..1190
                     /gene="MAPK8"
                     /gene_synonym="JNK; JNK-46; JNK1; JNK1A2; JNK21B1/2;
                     PRKM8; SAPK1; SAPK1c"
                     /inference="alignment:Splign:2.1.0"
     exon            1191..1254
                     /gene="MAPK8"
                     /gene_synonym="JNK; JNK-46; JNK1; JNK1A2; JNK21B1/2;
                     PRKM8; SAPK1; SAPK1c"
                     /inference="alignment:Splign:2.1.0"
     exon            1255..1332
                     /gene="MAPK8"
                     /gene_synonym="JNK; JNK-46; JNK1; JNK1A2; JNK21B1/2;
                     PRKM8; SAPK1; SAPK1c"
                     /inference="alignment:Splign:2.1.0"
     exon            1333..5809
                     /gene="MAPK8"
                     /gene_synonym="JNK; JNK-46; JNK1; JNK1A2; JNK21B1/2;
                     PRKM8; SAPK1; SAPK1c"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2305..2310
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MAPK8"
                     /gene_synonym="JNK; JNK-46; JNK1; JNK1A2; JNK21B1/2;
                     PRKM8; SAPK1; SAPK1c"
                     /note="hexamer: AATAAA"
     polyA_site      2330
                     /gene="MAPK8"
                     /gene_synonym="JNK; JNK-46; JNK1; JNK1A2; JNK21B1/2;
                     PRKM8; SAPK1; SAPK1c"
     regulatory      2855..2860
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MAPK8"
                     /gene_synonym="JNK; JNK-46; JNK1; JNK1A2; JNK21B1/2;
                     PRKM8; SAPK1; SAPK1c"
                     /note="hexamer: AGTAAA"
     polyA_site      2861
                     /gene="MAPK8"
                     /gene_synonym="JNK; JNK-46; JNK1; JNK1A2; JNK21B1/2;
                     PRKM8; SAPK1; SAPK1c"
                     /note="major polyA site"
     polyA_site      2880
                     /gene="MAPK8"
                     /gene_synonym="JNK; JNK-46; JNK1; JNK1A2; JNK21B1/2;
                     PRKM8; SAPK1; SAPK1c"
     regulatory      4029..4034
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MAPK8"
                     /gene_synonym="JNK; JNK-46; JNK1; JNK1A2; JNK21B1/2;
                     PRKM8; SAPK1; SAPK1c"
                     /note="hexamer: AATATA"
     polyA_site      4055
                     /gene="MAPK8"
                     /gene_synonym="JNK; JNK-46; JNK1; JNK1A2; JNK21B1/2;
                     PRKM8; SAPK1; SAPK1c"
     regulatory      5374..5379
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MAPK8"
                     /gene_synonym="JNK; JNK-46; JNK1; JNK1A2; JNK21B1/2;
                     PRKM8; SAPK1; SAPK1c"
                     /note="hexamer: AATAAA"
     polyA_site      5397
                     /gene="MAPK8"
                     /gene_synonym="JNK; JNK-46; JNK1; JNK1A2; JNK21B1/2;
                     PRKM8; SAPK1; SAPK1c"
     regulatory      5781..5786
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MAPK8"
                     /gene_synonym="JNK; JNK-46; JNK1; JNK1A2; JNK21B1/2;
                     PRKM8; SAPK1; SAPK1c"
                     /note="hexamer: AAGAAA"
     polyA_site      5809
                     /gene="MAPK8"
                     /gene_synonym="JNK; JNK-46; JNK1; JNK1A2; JNK21B1/2;
                     PRKM8; SAPK1; SAPK1c"
ORIGIN      
        1 gttactcagc cgagcggccg aggccggacg acgcggcttg gattgcggag ccgcgagcag
       61 cgctgggtaa cggccgcggc gaccaccccg gacggcccct gtccccgctg gcgggcttcc
      121 ctgtcgccgt tcgctgcgct gccggcttct tggtgaattt ttggatgaag ccattaaatt
      181 aattgcttgc catcatgagc agaagcaagc gtgacaacaa tttttatagt gtagagattg
      241 gagattctac attcacagtc ctgaaacgat atcagaattt aaaacctata ggctcaggag
      301 ctcaaggaat agtatgcgca gcttatgatg ccattcttga aagaaatgtt gcaatcaaga
      361 agctaagccg accatttcag aatcagactc atgccaagcg ggcctacaga gagctagttc
      421 ttatgaaatg tgttaatcac aaaaatataa ttggcctttt gaatgttttc acaccacaga
      481 aatccctaga agaatttcaa gatgtttaca tagtcatgga gctcatggat gcaaatcttt
      541 gccaagtgat tcagatggag ctagatcatg aaagaatgtc ctaccttctc tatcagatgc
      601 tgtgtggaat caagcacctt cattctgctg gaattattca tcgggactta aagcccagta
      661 atatagtagt aaaatctgat tgcactttga agattcttga cttcggtctg gccaggactg
      721 caggaacgag ttttatgatg acgccttatg tagtgactcg ctactacaga gcacccgagg
      781 tcatccttgg catgggctac aaggaaaacg tggatttatg gtctgtgggg tgcattatgg
      841 gagaaatggt ttgccacaaa atcctctttc caggaaggga ctatattgat cagtggaata
      901 aagttattga acagcttgga acaccatgtc ctgaattcat gaagaaactg caaccaacag
      961 taaggactta cgttgaaaac agacctaaat atgctggata tagctttgag aaactcttcc
     1021 ctgatgtcct tttcccagct gactcagaac acaacaaact taaagccagt caggcaaggg
     1081 atttgttatc caaaatgctg gtaatagatg catctaaaag gatctctgta gatgaagctc
     1141 tccaacaccc gtacatcaat gtctggtatg atccttctga agcagaagct ccaccaccaa
     1201 agatccctga caagcagtta gatgaaaggg aacacacaat agaagagtgg aaagaattga
     1261 tatataagga agttatggac ttggaggaga gaaccaagaa tggagttata cgggggcagc
     1321 cctctccttt aggtgcagca gtgatcaatg gctctcagca tccatcatca tcgtcgtctg
     1381 tcaatgatgt gtcttcaatg tcaacagatc cgactttggc ctctgataca gacagcagtc
     1441 tagaagcagc agctgggcct ctgggctgct gtagatgact acttgggcca tcggggggtg
     1501 ggagggatgg ggagtcggtt agtcattgat agaactactt tgaaaacaat tcagtggtct
     1561 tatttttggg tgatttttca aaaaatgtag aattcatttt gtagtaaagt agtttatttt
     1621 ttttaatttc aagtgatgta atttaaaacc taagttgtgt ttcaaaacag caacaaaact
     1681 gtattgtatt ttttttgctg taattaactg tataatgtaa acctaattat tttatcatgg
     1741 tttaaatttt ttgcatattt gctttatctt atgctgctga tttttttaac tgaatttgta
     1801 agattttgtt tatcaaagca actattatgt ggtgacttgc ctatatcatg aattatttaa
     1861 gatttttata gtttttttta attagaattt atttcagatg ttttgttcat gatactatcc
     1921 ttcagggtta tgtgcttatc aatgaaataa ccccagagga gtgagggaaa ataacttgta
     1981 gccagttata ttcaggaata actactgtaa atgatgaacg tgttaggaga cctccaatat
     2041 ttgctacttg ccaatcctaa tttagttaca agaattggta ggcaatccta cttaattttg
     2101 gcaaaagccc cgtcatctaa atggcagaat aactcagagc atgtctttga agatgctggg
     2161 cgtctaccac caccttatgt ccccacccta cccaacaaaa ataagtaaaa agaatatggt
     2221 gtattctaca aatttgtggc atgctcaaag tttatgatca cataaaggca agaggatact
     2281 tcatgaataa tacatttcaa tgcaaataaa cagatggttc acttctacta gctatgagcc
     2341 tgtttttgta tacactgagt taatctactc aggctgtagg tcccagcaat gttctagagt
     2401 ctggtctttc cctttcctgc agcttcgggt ccttggacct ttcctgtttc ctattacttg
     2461 gagtgtctgt cagttgagca ccagttgttc tggtgtttca tttgattcta cttgtagcat
     2521 aatcatttat acgagctatt gggaggttcc aaaccctacc tagatttgtg taggtgatgt
     2581 atcaaatgag caatataccg ttcatctgaa aatagtagca cacagccata tataggatat
     2641 cattttctaa ggactgtttc ttcacattga gcagagcagg cataaatggt ggttatttag
     2701 tctaagtctt ttattttttt atacctgatt ttcaacataa cacgcaatgt ggatgtcgag
     2761 tagtgttaag aatggtgctg ctcctgacaa gtgtatgtta actgtttaca ttttctatct
     2821 gtagaattat ttctctatta ctgaactttt cctaagtaaa atgtctttga agtctcgtta
     2881 tttctgaaat acgttgtctg taatagaccc aggcaccttt taaattatct ctggaacaag
     2941 agggatttca tgtaatgaac taggaaatgc atactcacat aagcaacaag gttctaggca
     3001 gaaagcccct tggaatttgt gaccaacagg agcaagaaca ggtgcggctc aacatgcaat
     3061 gtctgaaaat ttgcttggca ttttattcat atatttagtg caaaattatt tttgagtgag
     3121 atattttaca tcactgttaa tgtgcaatat ttaagattaa aatacattag cttttttata
     3181 tactttgaag tagcaagttt gttttcgatg gcttagagtc atgatttcca gcttcccagc
     3241 ctttttatca gtcccttttc taatacaaca aggtgcatta atttgattag gcaaattaga
     3301 gttctaagac acttcttgaa ttgtagacag aaaatattgg attcacaatt tcagcagaaa
     3361 tttgagaatg agtgtgttta tattaatttc acaattagct gtattttctg tagcatagat
     3421 tatgtcactg ttgcactttc acagcagaca tgctttcaga aggttctcat attttatgtt
     3481 tgattgctga taagccatct ctattgatac agattttggt taagtaagga aaaccaggtg
     3541 tgtgtctgta tcatttattg taaatgccag ctgccacttg ccaaccatca tgttcagttc
     3601 aattcaaaga aaacaaactc tcattactta gtgtaaacta aaatacttaa caaattatat
     3661 cctaaaaaca aggtctcttt gttaaatgtt gcatgcccta ggttttaaat tactacatcc
     3721 aaatacagtt ttcgtcttaa atttgttaag ctaaatatat gttggttctt tttattttgg
     3781 aatcctttaa gcatcttaaa catttttttt ttgaagagaa gttacaaata acatttctat
     3841 caggtagtac ttgtatgaaa ccacctttct tattctataa ttttgatttt tcaattttat
     3901 atacttaata tactcactgt cttactatca gaaagttatt ttgaccaaga tttttattat
     3961 cttcatagat tcagaaagag atgctaattc tgtaccaatg tcttcctggt tactattctc
     4021 ttccctctaa tatatactgg ccatttgtaa aaccattgtg ttgttgggat cacttagtta
     4081 tactatacgc agatagagca tctcaactct gtcatagtgt ttgctgaaca gttttcagtg
     4141 tcatgcacct ttggctgcta attgttcctg acgtgcactc ttccgagttg gtaaaggcac
     4201 agtgtgttca tgccagactt ctaagagaaa caccagcctc ttaaatcaga agcctacaca
     4261 caaccccctt aacaatccaa agaagcttga tggtgtgcaa agaagcatcc tgccagcctt
     4321 gtcattgttc tgttctatgc taatcctgct gtgttgtcta aaagatggag ggaagaggac
     4381 atcagtgtct gatagtgaaa tcatcagcag gaaagtgaag ctctttcctt ggttacagat
     4441 aagacttggt ttacactatt ggccagtatc tgctaaacat atgaagactt aactattcag
     4501 tgttgcctag gcattcgcct gcacaacatt ttgaggttag aacatagaat attttcagaa
     4561 atactgttgt agtttgtgag tgttgttcat tagttacaca ttagctatag agtggatgca
     4621 tgaagcccca tgacaccagt aaacttctct taccagtagg taaaccaaac accattctgt
     4681 cattagcagc cctcttaaat gttgcctctc cgtatcctgt tgcatttttg tgtgcattgt
     4741 gtttctactg atctctctta ggtttttacg gaatcaaagg aaactaattt ttccttaata
     4801 gcaagaaaga tgaagaggta aagggcattg aagcagaaat gtatagtttg gggtacgatt
     4861 agaaaactcg taaggaaaac agaagtccta atttcaaact gactgctctt cgttaagtgc
     4921 tcttaaggag agtctagtaa cagtaacact ttctggccat ttctagttta gattctcttc
     4981 gttactgaaa cttttgagaa atattacctg tggattaatt ttgcacaatg ttctattctc
     5041 ataatgactt acaaattaaa ctaggttttt attgaactac ctcacactaa ttttctatgc
     5101 tttcccaagt aagctgttgc cctgttagat ctttactgag tgaattataa atgtgtgtta
     5161 aatactttct agccaatgtt gacacaatac cagtaagtat gtaaagtata taccttacat
     5221 cagtaagaga cacgtgtaaa atctttgact gtatgtcttg caaaattgtg ctcgttgaca
     5281 ttattactgt ttttgtaagt agaaacctgc tcgtgatatc ggtccattta cattttacaa
     5341 aaggagtaaa tcttagtaaa aattttacga agaaataaat tacttttgta ggcccaatat
     5401 ttggtatatt tttgagaagc tgttaatctt ttagctgaat aatgaagtta gactgaatta
     5461 cgtgtctccc tggactgtga catctatttt ctcattacag tttatcctgg tcagcagggt
     5521 gtcacacctg gaaacctgag tatgatagct gacatttgct tttctccctc tgcgatgtca
     5581 ttcctcctcc attcctctcc ttccctgtgt tccgttccct ctcctttcct ctagacaaaa
     5641 caaaatgggg cactttttag ggaatgctga gatcattatt gtggtttttc atcattcatg
     5701 ccctagtcat taaacatgca ccactggaat gtaaacaatg ttatctagta tgtcaattgg
     5761 ttataatatt ttaaataaaa aagaaaaaag tggtatgaaa attatgaaa
//
