LOCUS       NM_001384493            7808 bp    mRNA    linear   PRI 20-AUG-2024
DEFINITION  Homo sapiens mitogen-activated protein kinase kinase kinase kinase
            4 (MAP4K4), transcript variant 21, mRNA.
ACCESSION   NM_001384493 XM_017005350
VERSION     NM_001384493.1
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 7808)
  AUTHORS   Ampadu,F., Awasthi,V. and Joshi,A.D.
  TITLE     Role of Mitogen-Activated Protein Kinase Kinase Kinase Kinase 4
            Signaling in Liver and Metabolic Diseases
  JOURNAL   J Pharmacol Exp Ther 390 (2), 233-239 (2024)
   PUBMED   38844365
  REMARK    GeneRIF: Role of Mitogen-Activated Protein Kinase Kinase Kinase
            Kinase 4 Signaling in Liver and Metabolic Diseases.
            Review article
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7808)
  AUTHORS   Chen,Y., Li,S., Guan,B., Yan,X., Huang,C., Du,Y., Yang,F.,
            Zhang,N., Li,Y., Lu,J., Wang,J., Zhang,J., Chen,Z., Chen,C. and
            Kong,X.
  TITLE     MAP4K4 exacerbates cardiac microvascular injury in diabetes by
            facilitating S-nitrosylation modification of Drp1
  JOURNAL   Cardiovasc Diabetol 23 (1), 164 (2024)
   PUBMED   38724987
  REMARK    GeneRIF: MAP4K4 exacerbates cardiac microvascular injury in
            diabetes by facilitating S-nitrosylation modification of Drp1.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 7808)
  AUTHORS   LeClaire,L.L., Rana,M., Baumgartner,M. and Barber,D.L.
  TITLE     The Nck-interacting kinase NIK increases Arp2/3 complex activity by
            phosphorylating the Arp2 subunit
  JOURNAL   J Cell Biol 208 (2), 161-170 (2015)
   PUBMED   25601402
REFERENCE   4  (bases 1 to 7808)
  AUTHORS   Santhana Kumar,K., Tripolitsioti,D., Ma,M., Grahlert,J., Egli,K.B.,
            Fiaschetti,G., Shalaby,T., Grotzer,M.A. and Baumgartner,M.
  TITLE     The Ser/Thr kinase MAP4K4 drives c-Met-induced motility and
            invasiveness in a cell-based model of SHH medulloblastoma
  JOURNAL   Springerplus 4, 19 (2015)
   PUBMED   25625039
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 7808)
  AUTHORS   Ma,M. and Baumgartner,M.
  TITLE     Intracellular Theileria annulata promote invasive cell motility
            through kinase regulation of the host actin cytoskeleton
  JOURNAL   PLoS Pathog 10 (3), e1004003 (2014)
   PUBMED   24626571
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 7808)
  AUTHORS   Machida,N., Umikawa,M., Takei,K., Sakima,N., Myagmar,B.E.,
            Taira,K., Uezato,H., Ogawa,Y. and Kariya,K.
  TITLE     Mitogen-activated protein kinase kinase kinase kinase 4 as a
            putative effector of Rap2 to activate the c-Jun N-terminal kinase
  JOURNAL   J Biol Chem 279 (16), 15711-15714 (2004)
   PUBMED   14966141
  REMARK    GeneRIF: MAP4K4 is a putative effector of Rap2, a Ras family small
            GTP-binding protein, mediating the activation of JNK by Rap2
REFERENCE   7  (bases 1 to 7808)
  AUTHORS   Wright,J.H., Wang,X., Manning,G., LaMere,B.J., Le,P., Zhu,S.,
            Khatry,D., Flanagan,P.M., Buckley,S.D., Whyte,D.B., Howlett,A.R.,
            Bischoff,J.R., Lipson,K.E. and Jallal,B.
  TITLE     The STE20 kinase HGK is broadly expressed in human tumor cells and
            can modulate cellular transformation, invasion, and adhesion
  JOURNAL   Mol Cell Biol 23 (6), 2068-2082 (2003)
   PUBMED   12612079
  REMARK    GeneRIF: The STE20 kinase HGK is broadly expressed in human tumor
            cells and can modulate cellular transformation, invasion, and
            adhesion.
REFERENCE   8  (bases 1 to 7808)
  AUTHORS   Becker,E., Huynh-Do,U., Holland,S., Pawson,T., Daniel,T.O. and
            Skolnik,E.Y.
  TITLE     Nck-interacting Ste20 kinase couples Eph receptors to c-Jun
            N-terminal kinase and integrin activation
  JOURNAL   Mol Cell Biol 20 (5), 1537-1545 (2000)
   PUBMED   10669731
REFERENCE   9  (bases 1 to 7808)
  AUTHORS   Yao,Z., Zhou,G., Wang,X.S., Brown,A., Diener,K., Gan,H. and
            Tan,T.H.
  TITLE     A novel human STE20-related protein kinase, HGK, that specifically
            activates the c-Jun N-terminal kinase signaling pathway
  JOURNAL   J Biol Chem 274 (4), 2118-2125 (1999)
   PUBMED   9890973
REFERENCE   10 (bases 1 to 7808)
  AUTHORS   Su,Y.C., Han,J., Xu,S., Cobb,M. and Skolnik,E.Y.
  TITLE     NIK is a new Ste20-related kinase that binds NCK and MEKK1 and
            activates the SAPK/JNK cascade via a conserved regulatory domain
  JOURNAL   EMBO J 16 (6), 1279-1290 (1997)
   PUBMED   9135144
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC007005.3 and AC005035.2.
            
            On Jun 18, 2020 this sequence version replaced XM_017005350.1.
            
            Summary: The protein encoded by this gene is a member of the
            serine/threonine protein kinase family. This kinase has been shown
            to specifically activate MAPK8/JNK. The activation of MAPK8 by this
            kinase is found to be inhibited by the dominant-negative mutants of
            MAP3K7/TAK1, MAP2K4/MKK4, and MAP2K7/MKK7, which suggests that this
            kinase may function through the MAP3K7-MAP2K4-MAP2K7 kinase
            cascade, and mediate the TNF-alpha signaling pathway. Alternatively
            spliced transcript variants encoding different isoforms have been
            identified. [provided by RefSeq, Jul 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            RNAseq introns :: single sample supports all introns SAMEA1965299,
                              SAMEA1968189 [ECO:0000348]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-431               AC007005.3         56920-57350
            432-497             AC007005.3         57686-57751
            498-554             AC007005.3         149933-149989
            555-680             AC005035.2         15565-15690
            681-791             AC005035.2         16956-17066
            792-882             AC005035.2         21141-21231
            883-1013            AC005035.2         23358-23488
            1014-1068           AC005035.2         26046-26100
            1069-1147           AC005035.2         27537-27615
            1148-1323           AC005035.2         31456-31631
            1324-1396           AC005035.2         34246-34318
            1397-1607           AC005035.2         35738-35948
            1608-1769           AC005035.2         47614-47775
            1770-1856           AC005035.2         50633-50719
            1857-2078           AC005035.2         51280-51501
            2079-2309           AC005035.2         55458-55688
            2310-2416           AC005035.2         56567-56673
            2417-2568           AC005035.2         58065-58216
            2569-2666           AC005035.2         58849-58946
            2667-2675           AC005035.2         59666-59674
            2676-2851           AC005035.2         61259-61434
            2852-2972           AC005035.2         61932-62052
            2973-3164           AC005035.2         63131-63322
            3165-3282           AC005035.2         65284-65401
            3283-3453           AC005035.2         65719-65889
            3454-3597           AC005035.2         68640-68783
            3598-3732           AC005035.2         74188-74322
            3733-3833           AC005035.2         76824-76924
            3834-3983           AC005035.2         78725-78874
            3984-4143           AC005035.2         79415-79574
            4144-4283           AC005035.2         80433-80572
            4284-7808           AC005035.2         82803-86327
FEATURES             Location/Qualifiers
     source          1..7808
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="2"
                     /map="2q11.2"
     gene            1..7808
                     /gene="MAP4K4"
                     /gene_synonym="FLH21957; HEL-S-31; HGK; MEKKK4; NIK"
                     /note="mitogen-activated protein kinase kinase kinase
                     kinase 4"
                     /db_xref="GeneID:9448"
                     /db_xref="HGNC:HGNC:6866"
                     /db_xref="MIM:604666"
     exon            1..431
                     /gene="MAP4K4"
                     /gene_synonym="FLH21957; HEL-S-31; HGK; MEKKK4; NIK"
                     /inference="alignment:Splign:2.1.0"
     CDS             375..4367
                     /gene="MAP4K4"
                     /gene_synonym="FLH21957; HEL-S-31; HGK; MEKKK4; NIK"
                     /EC_number="2.7.11.1"
                     /note="isoform 21 is encoded by transcript variant 21;
                     hepatocyte progenitor kinase-like/germinal center
                     kinase-like kinase; Ste20 group protein kinase HGK;
                     epididymis secretory protein Li 31; MEK kinase kinase 4;
                     nck-interacting kinase; HPK/GCK-like kinase HGK; MAPK/ERK
                     kinase kinase kinase 4"
                     /codon_start=1
                     /product="mitogen-activated protein kinase kinase kinase
                     kinase 4 isoform 21"
                     /protein_id="NP_001371422.1"
                     /db_xref="GeneID:9448"
                     /db_xref="HGNC:HGNC:6866"
                     /db_xref="MIM:604666"
                     /translation="MANDSPAKSLVDIDLSSLRDPAGIFELVEVVGNGTYGQVYKGRH
                     VKTGQLAAIKVMDVTEDEEEEIKLEINMLKKYSHHRNIATYYGAFIKKSPPGHDDQLW
                     LVMEFCGAGSITDLVKNTKGNTLKEDWIAYISREILRGLAHLHIHHVIHRDIKGQNVL
                     LTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDLWS
                     CGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFFSFIEGCLVKNYMQ
                     RPSTEQLLKHPFIRDQPNERQVRIQLKDHIDRTRKKRGEKDETEYEYSGSEEEEEEVP
                     EQEGEPSSIVNVPGESTLRRDFLRLQQENKERSEALRRQQLLQEQQLREQEEYKRQLL
                     AERQKRIEQQKEQRRRLEEQQRREREARRQQEREQRRREQEEKRRLEELERRRKEEEE
                     RRRAEEEKRRVEREQEYIRRQLEEEQRHLEVLQQQLLQEQAMLLECRWREMEEHRQAE
                     RLQRQLQQEQAYLLSLQHDHRRPHPQHSQQPPPPQQERSKPSFHAPEPKAHYEPADRA
                     REVQWSHLASLKNNVSPVSRSHSFSDPSPKFAHHHLRSQDPCPPSRSEVLSQSSDSKS
                     EAPDPTQKAWSRSDSDEVPPRVPVRTTSRSPVLSRRDSPLQGSGQQNSQAGQRNSTSS
                     IEPRLLWERVEKLVPRPGSGSSSGSSNSGSQPGSHPGSQSGSGERFRVRSSSKSEGSP
                     SQRLENAVKKPEDKKEVFRPLKPAGEVDLTALAKELRAVEDVRPPHKVTDYSSSSEES
                     GTTDEEDDDVEQEGADESTSGPEDTRAASSLNLSNGETESVKTMIVHDDVESEPAMTP
                     SKEGTLIVRQSTVDQKRASHHESNGFAGRIHLLPDLLQQSHSSSTSSTSSSPSSSQPT
                     PTMSPQTPQDKLTANETQSASSTLQKHKSSSSFTPFIDPRLLQISPSSGTTVTSVVGF
                     SCDGMRPEAIRQDPTRKGSVVNVNPTNTRPQSDTPEIRKYKKRFNSEILCAALWGVNL
                     LVGTESGLMLLDRSGQGKVYPLINRRRFQQMDVLEGLNVLVTISGKKDKLRVYYLSWL
                     RNKILHNDPEVEKKQGWTTVGDLEGCVHYKVVKYERIKFLVIALKSSVEVYAWAPKPY
                     HKFMAFKSFGELVHKPLLVDLTVEEGQRLKVIYGSCAGFHAVDVDSGSVYDIYLPTHI
                     QCSIKPHAIIILPNTDGMELLVCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIRSN
                     QTMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFM
                     TLGRTSLLSW"
     exon            432..497
                     /gene="MAP4K4"
                     /gene_synonym="FLH21957; HEL-S-31; HGK; MEKKK4; NIK"
                     /inference="alignment:Splign:2.1.0"
     exon            498..554
                     /gene="MAP4K4"
                     /gene_synonym="FLH21957; HEL-S-31; HGK; MEKKK4; NIK"
                     /inference="alignment:Splign:2.1.0"
     exon            555..680
                     /gene="MAP4K4"
                     /gene_synonym="FLH21957; HEL-S-31; HGK; MEKKK4; NIK"
                     /inference="alignment:Splign:2.1.0"
     exon            681..791
                     /gene="MAP4K4"
                     /gene_synonym="FLH21957; HEL-S-31; HGK; MEKKK4; NIK"
                     /inference="alignment:Splign:2.1.0"
     exon            792..882
                     /gene="MAP4K4"
                     /gene_synonym="FLH21957; HEL-S-31; HGK; MEKKK4; NIK"
                     /inference="alignment:Splign:2.1.0"
     exon            883..1013
                     /gene="MAP4K4"
                     /gene_synonym="FLH21957; HEL-S-31; HGK; MEKKK4; NIK"
                     /inference="alignment:Splign:2.1.0"
     exon            1014..1068
                     /gene="MAP4K4"
                     /gene_synonym="FLH21957; HEL-S-31; HGK; MEKKK4; NIK"
                     /inference="alignment:Splign:2.1.0"
     exon            1069..1147
                     /gene="MAP4K4"
                     /gene_synonym="FLH21957; HEL-S-31; HGK; MEKKK4; NIK"
                     /inference="alignment:Splign:2.1.0"
     exon            1148..1323
                     /gene="MAP4K4"
                     /gene_synonym="FLH21957; HEL-S-31; HGK; MEKKK4; NIK"
                     /inference="alignment:Splign:2.1.0"
     exon            1324..1396
                     /gene="MAP4K4"
                     /gene_synonym="FLH21957; HEL-S-31; HGK; MEKKK4; NIK"
                     /inference="alignment:Splign:2.1.0"
     exon            1397..1607
                     /gene="MAP4K4"
                     /gene_synonym="FLH21957; HEL-S-31; HGK; MEKKK4; NIK"
                     /inference="alignment:Splign:2.1.0"
     exon            1608..1769
                     /gene="MAP4K4"
                     /gene_synonym="FLH21957; HEL-S-31; HGK; MEKKK4; NIK"
                     /inference="alignment:Splign:2.1.0"
     exon            1770..1856
                     /gene="MAP4K4"
                     /gene_synonym="FLH21957; HEL-S-31; HGK; MEKKK4; NIK"
                     /inference="alignment:Splign:2.1.0"
     exon            1857..2078
                     /gene="MAP4K4"
                     /gene_synonym="FLH21957; HEL-S-31; HGK; MEKKK4; NIK"
                     /inference="alignment:Splign:2.1.0"
     exon            2079..2309
                     /gene="MAP4K4"
                     /gene_synonym="FLH21957; HEL-S-31; HGK; MEKKK4; NIK"
                     /inference="alignment:Splign:2.1.0"
     exon            2310..2416
                     /gene="MAP4K4"
                     /gene_synonym="FLH21957; HEL-S-31; HGK; MEKKK4; NIK"
                     /inference="alignment:Splign:2.1.0"
     exon            2417..2568
                     /gene="MAP4K4"
                     /gene_synonym="FLH21957; HEL-S-31; HGK; MEKKK4; NIK"
                     /inference="alignment:Splign:2.1.0"
     exon            2569..2666
                     /gene="MAP4K4"
                     /gene_synonym="FLH21957; HEL-S-31; HGK; MEKKK4; NIK"
                     /inference="alignment:Splign:2.1.0"
     exon            2667..2675
                     /gene="MAP4K4"
                     /gene_synonym="FLH21957; HEL-S-31; HGK; MEKKK4; NIK"
                     /inference="alignment:Splign:2.1.0"
     exon            2676..2851
                     /gene="MAP4K4"
                     /gene_synonym="FLH21957; HEL-S-31; HGK; MEKKK4; NIK"
                     /inference="alignment:Splign:2.1.0"
     exon            2852..2972
                     /gene="MAP4K4"
                     /gene_synonym="FLH21957; HEL-S-31; HGK; MEKKK4; NIK"
                     /inference="alignment:Splign:2.1.0"
     exon            2973..3164
                     /gene="MAP4K4"
                     /gene_synonym="FLH21957; HEL-S-31; HGK; MEKKK4; NIK"
                     /inference="alignment:Splign:2.1.0"
     exon            3165..3282
                     /gene="MAP4K4"
                     /gene_synonym="FLH21957; HEL-S-31; HGK; MEKKK4; NIK"
                     /inference="alignment:Splign:2.1.0"
     exon            3283..3453
                     /gene="MAP4K4"
                     /gene_synonym="FLH21957; HEL-S-31; HGK; MEKKK4; NIK"
                     /inference="alignment:Splign:2.1.0"
     exon            3454..3597
                     /gene="MAP4K4"
                     /gene_synonym="FLH21957; HEL-S-31; HGK; MEKKK4; NIK"
                     /inference="alignment:Splign:2.1.0"
     exon            3598..3732
                     /gene="MAP4K4"
                     /gene_synonym="FLH21957; HEL-S-31; HGK; MEKKK4; NIK"
                     /inference="alignment:Splign:2.1.0"
     exon            3733..3833
                     /gene="MAP4K4"
                     /gene_synonym="FLH21957; HEL-S-31; HGK; MEKKK4; NIK"
                     /inference="alignment:Splign:2.1.0"
     exon            3834..3983
                     /gene="MAP4K4"
                     /gene_synonym="FLH21957; HEL-S-31; HGK; MEKKK4; NIK"
                     /inference="alignment:Splign:2.1.0"
     exon            3984..4143
                     /gene="MAP4K4"
                     /gene_synonym="FLH21957; HEL-S-31; HGK; MEKKK4; NIK"
                     /inference="alignment:Splign:2.1.0"
     exon            4144..4283
                     /gene="MAP4K4"
                     /gene_synonym="FLH21957; HEL-S-31; HGK; MEKKK4; NIK"
                     /inference="alignment:Splign:2.1.0"
     exon            4284..7808
                     /gene="MAP4K4"
                     /gene_synonym="FLH21957; HEL-S-31; HGK; MEKKK4; NIK"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4572..4577
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MAP4K4"
                     /gene_synonym="FLH21957; HEL-S-31; HGK; MEKKK4; NIK"
                     /note="hexamer: AATAAA"
     polyA_site      4614
                     /gene="MAP4K4"
                     /gene_synonym="FLH21957; HEL-S-31; HGK; MEKKK4; NIK"
                     /note="major polyA site"
     regulatory      5902..5907
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MAP4K4"
                     /gene_synonym="FLH21957; HEL-S-31; HGK; MEKKK4; NIK"
                     /note="hexamer: AATAAA"
     polyA_site      5922
                     /gene="MAP4K4"
                     /gene_synonym="FLH21957; HEL-S-31; HGK; MEKKK4; NIK"
     regulatory      7787..7792
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MAP4K4"
                     /gene_synonym="FLH21957; HEL-S-31; HGK; MEKKK4; NIK"
                     /note="hexamer: AATAAA"
     polyA_site      7808
                     /gene="MAP4K4"
                     /gene_synonym="FLH21957; HEL-S-31; HGK; MEKKK4; NIK"
ORIGIN      
        1 actcgctcaa ctcggcgccg ccgcggcccc acgctccggg cccgtcctcg aggcgcgcgg
       61 cgcggggcgc gggcgccggg gcctgaggcg gcgggcgacg cccgggggcc tgacggccgg
      121 ccccgcgcca tggtgtgagc gccgccgccc gtgcacgctc cgtccgccct ccgcgcggcc
      181 cggccggcag agagccccga gcggcccgag agcgcagccg agcccgccgc cgccgcccgc
      241 ggccccgcga ggagagtacc gggccggctc ggctgccgcg cgaggagcgc ggtcggcggc
      301 ctggtctgcg gctgagatac acagagcgac agagacattt attgttattt gttttttggt
      361 ggcaaaaagg gaaaatggcg aacgactccc ctgcaaaaag tctggtggac atcgacctct
      421 cctccctgcg ggatcctgct gggatttttg agctggtgga agtggttgga aatggcacct
      481 atggacaagt ctataagggt cgacatgtta aaacgggtca gttggcagcc atcaaagtta
      541 tggatgtcac tgaggatgaa gaggaagaaa tcaaactgga gataaatatg ctaaagaaat
      601 actctcatca cagaaacatt gcaacatatt atggtgcttt catcaaaaag agccctccag
      661 gacatgatga ccaactctgg cttgttatgg agttctgtgg ggctgggtcc attacagacc
      721 ttgtgaagaa caccaaaggg aacacactca aagaagactg gatcgcttac atctccagag
      781 aaatcctgag gggactggca catcttcaca ttcatcatgt gattcaccgg gatatcaagg
      841 gccagaatgt gttgctgact gagaatgcag aggtgaaact tgttgacttt ggtgtgagtg
      901 ctcagctgga caggactgtg gggcggagaa atacgttcat aggcactccc tactggatgg
      961 ctcctgaggt catcgcctgt gatgagaacc cagatgccac ctatgattac agaagtgatc
     1021 tttggtcttg tggcattaca gccattgaga tggcagaagg tgctccccct ctctgtgaca
     1081 tgcatccaat gagagcactg tttctcattc ccagaaaccc tcctccccgg ctgaagtcaa
     1141 aaaaatggtc gaagaagttt tttagtttta tagaagggtg cctggtgaag aattacatgc
     1201 agcggccctc tacagagcag cttttgaaac atccttttat aagggatcag ccaaatgaaa
     1261 ggcaagttag aatccagctt aaggatcata tagatcgtac caggaagaag agaggcgaga
     1321 aagatgaaac tgagtatgag tacagtggga gtgaggaaga agaggaggaa gtgcctgaac
     1381 aggaaggaga gccaagttcc attgtgaacg tgcctggtga gtctactctt cgccgagatt
     1441 tcctgagact gcagcaggag aacaaggaac gttccgaggc tcttcggaga caacagttac
     1501 tacaggagca acagctccgg gagcaggaag aatataaaag gcaactgctg gcagagagac
     1561 agaagcggat tgagcagcag aaagaacaga ggcgacggct agaagagcaa caaaggagag
     1621 agcgggaagc tagaaggcag caggaacgtg aacagcgaag gagagaacaa gaagaaaaga
     1681 ggcgtctaga ggagttggag agaaggcgca aagaagaaga ggagaggaga cgggcagaag
     1741 aagaaaagag gagagttgaa agagaacagg agtatatcag gcgacagcta gaagaggagc
     1801 agcggcactt ggaagtcctt cagcagcagc tgctccagga gcaggccatg ttactggagt
     1861 gccgatggcg ggagatggag gagcaccggc aggcagagag gctccagagg cagttgcaac
     1921 aagaacaagc atatctcctg tctctacagc atgaccatag gaggccgcac ccgcagcact
     1981 cgcagcagcc gccaccaccg cagcaggaaa ggagcaagcc aagcttccat gctcccgagc
     2041 ccaaagccca ctacgagcct gctgaccgag cgcgagaggt acagtggtcc cacctggcat
     2101 ctctcaagaa caatgtttcc cctgtctcgc gatcccattc cttcagtgac ccttctccca
     2161 aatttgcaca ccaccatctt cgttctcagg acccatgtcc accttcccgc agtgaggtgc
     2221 tcagtcagag ctctgactct aagtcagagg cgcctgaccc tacccaaaag gcttggtcta
     2281 gatcagacag tgacgaggtg cctccaaggg ttcctgtgag aacaacatct cgctcccctg
     2341 ttctgtcccg tcgagattcc ccactgcagg gcagtgggca gcagaatagc caggcaggac
     2401 agagaaactc caccagcagt attgagccca ggcttctgtg ggagagagtg gagaagctgg
     2461 tgcccagacc tggcagtggc agctcctcag ggtccagcaa ctcaggatcc cagcccgggt
     2521 ctcaccctgg gtctcagagt ggctccgggg aacgcttcag agtgagatca tcatccaagt
     2581 ctgaaggctc tccatctcag cgcctggaaa atgcagtgaa aaaacctgaa gataaaaagg
     2641 aagttttcag acccctcaag cctgctggcg aagtggatct gaccgcactg gccaaagagc
     2701 ttcgagcagt ggaagatgta cggccacctc acaaagtaac ggactactcc tcatccagtg
     2761 aggagtcggg gacgacggat gaggaggacg acgatgtgga gcaggaaggg gctgacgagt
     2821 ccacctcagg accagaggac accagagcag cgtcatctct gaatttgagc aatggtgaaa
     2881 cggaatctgt gaaaaccatg attgtccatg atgatgtaga aagtgagccg gccatgaccc
     2941 catccaagga gggcactcta atcgtccgcc agagtacagt tgaccaaaag cgtgccagcc
     3001 atcatgagag caatggcttt gccggtcgca ttcacctctt gccagatctc ttacagcaaa
     3061 gccattcctc ctccacttcc tccacctcct cctccccatc ctccagccag ccgacaccca
     3121 ccatgtcccc acagacaccc caggacaagc tcactgctaa tgagactcag tccgctagta
     3181 gcacactcca gaaacacaaa tcttcctcct cctttacacc ttttatagac cccagattac
     3241 tacagatttc tccatctagc ggaacaacag tgacatctgt ggtgggattt tcctgtgatg
     3301 ggatgagacc agaagccata aggcaagatc ctacccggaa aggctcagtg gtcaatgtga
     3361 atcctaccaa cactaggcca cagagtgaca ccccggagat tcgtaaatac aagaagaggt
     3421 ttaactctga gattctgtgt gctgccttat ggggagtgaa tttgctagtg ggtacagaga
     3481 gtggcctgat gctgctggac agaagtggcc aagggaaggt ctatcctctt atcaaccgaa
     3541 gacgatttca acaaatggac gtacttgagg gcttgaatgt cttggtgaca atatctggca
     3601 aaaaggataa gttacgtgtc tactatttgt cctggttaag aaataaaata cttcacaatg
     3661 atccagaagt tgagaagaag cagggatgga caaccgtagg ggatttggaa ggatgtgtac
     3721 attataaagt tgtaaaatat gaaagaatca aatttctggt gattgctttg aagagttctg
     3781 tggaagtcta tgcgtgggca ccaaagccat atcacaaatt tatggccttt aagtcatttg
     3841 gagaattggt acataagcca ttactggtgg atctcactgt tgaggaaggc cagaggttga
     3901 aagtgatcta tggatcctgt gctggattcc atgctgttga tgtggattca ggatcagtct
     3961 atgacattta tctaccaaca catatccagt gtagcatcaa accccatgca atcatcatcc
     4021 tccccaatac agatggaatg gagcttctgg tgtgctatga agatgagggg gtttatgtaa
     4081 acacatatgg aaggatcacc aaggatgtag ttctacagtg gggagagatg cctacatcag
     4141 tagcatatat tcgatccaat cagacaatgg gctggggaga gaaggccata gagatccgat
     4201 ctgtggaaac tggtcacttg gatggtgtgt tcatgcacaa aagggctcaa agactaaaat
     4261 tcttgtgtga acgcaatgac aaggtgttct ttgcctctgt tcggtctggt ggcagcagtc
     4321 aggtttattt catgacctta ggcaggactt ctcttctgag ctggtagaag cagtgtgatc
     4381 cagggattac tggcctccag agtcttcaag atcctgagaa cttggaattc cttgtaactg
     4441 gagctcggag ctgcaccgag ggcaaccagg acagctgtgt gtgcagacct catgtgttgg
     4501 gttctctccc ctccttcctg ttcctcttat ataccagttt atccccattc tttttttttt
     4561 tcttactcca aaataaatca aggctgcaat gcagctggtg ctgttcagat tctaccatca
     4621 ggtgctataa gtgtttggga ttgagcatca tactggaaag caaacacctt tcctccagct
     4681 ccagaattcc ttgtctctga atgactctgt cttgtgggtg tctgacagtg gcgacgatga
     4741 acatgccgtt ggttttattg gcagtgggca caaggaggtg agaagtggtg gtaaaaggag
     4801 cggagtgctg aagcagagag cagatttaat atagtaacat taacagtgta tttaattgac
     4861 atttcttttt tgtaatgtga cgatatgtgg acaaagaaga agatgcaggt ttaagaagtt
     4921 aatatttata aaatgtgaaa gacacagtta ctaggataac ttttttgtgg gtggggcttg
     4981 ggagatgggg tggggtgggt taaggggtcc cattttgttt ctttggattt ggggtggggg
     5041 tcctggccaa gaactcagtc atttttctgt gtaccaggtt gcctaaatca tgtgcagatg
     5101 gttctaaaaa aaaaaaaaaa aaaaaaaaaa aaaggaaaaa aaaaaagaaa aagaaaacgt
     5161 gtgcattttg tataatggcc agaactttgt cgtgtgacag tattagcact gcctcagtta
     5221 aaggtttaat ttttgtttaa acctagacgt gcaacaaaag ttttaccaca gtctgcactt
     5281 gcagaagaaa gaaaaaaatt caaaccacat gtttattttt tttttgccta cctcattgtt
     5341 cttaatgcat tgagaggtga tttagtttat atgtttttgg aagaaaccat taatgtttaa
     5401 tttaatctta ataccaaaac gaccagattg aagtttgact tttattgtca caaatcagca
     5461 ggcacaagaa ctgtccatga agatgggaaa tagccttaag gctgatgcag tttacttaca
     5521 agtttagaaa ccagaatgct ttgtttttac cagattcacc attagaggtt gatggggcaa
     5581 ctgcagccca tgacacaaga tctcattgtt ctcgatgtag aggggttggt agcagacagg
     5641 tggttacatt agaatagtca cacaaactgt tcagtgttgc aggaaccttt tcttgggggt
     5701 gggggagttt cccttttcta aaaatgcaat gcactaaaac tattttaaga atgtagttaa
     5761 ttctgcttat tcataaagtg ggcatcttct gtgttttagg tgtaatatcg aagtcctggc
     5821 ttttctcgtt ttctcacttg ctctcttgtt ctctgttttt ttaaaccaat tttactttat
     5881 gaatatattc atgacatttg taataaatgt cttgagaaag aatttgtttc atggcttcat
     5941 ggtcatcact caagctcccg taaggatatt accgtctcag gaaaggatca ggactccatg
     6001 tcacagtcct gccatcttac tttcctcttg tcgagttctg agtggaaata actgcattat
     6061 ggctgcttta acctcagtca tcaaaagaaa cttgctgttt tttaggcttg atctttttcc
     6121 tttgtggtta attttcctgt atattgtgaa aatgggggat tttccctctg ctcccaccca
     6181 cctaaacaca gcagccattt gtacctgttt gcttcccatc ccacttggca cccactctga
     6241 cctcttgtca gtttcctgtt cctggttcca tctttttgaa aaaggccctc ctttgagcta
     6301 caaacatctg gtaagacaag tacatccact catgaatgca gacacagcag ctggtggttt
     6361 tgtgtatacc tgtaaagaca agctgagaag cttacttttt ggggaagtaa aagaagatgg
     6421 aaatggatgt ttcatttgta tgagtttgga gcagtgctga aggccaaagc cgcctactgg
     6481 tttgtagtta acctagagaa ggttgaaaaa ttaatcctac ctttaaaggg atttgaggta
     6541 ggctggattc catcgccaca ggactttagt tagaattaaa ttcctgcttg taatttatat
     6601 ccatgtttag gcttttcata agatgaaaca tgccacagtg aacacactcg tgtacatatc
     6661 aagagaagaa ggaaaggcac aggtggagaa cagtaaaagg tgggcagatg tctttgaaga
     6721 aatgctcaat gtctgatgct aagtgggaga aggcagagaa caaaggatgt ggcataatgg
     6781 tcttaacatt atccaaagac ttgaagctcc atgtctgtaa gtcaaatgtt acacaaaaaa
     6841 aaatgcaaat ggtgtttcat tggaattacc aagtgcttag aacttgctgg ctttcccata
     6901 ggtggtaaag gggtctgagc tcacaccgag ttgtgcttgg cttgcttgtg cagctccagg
     6961 cacccggtgg gcactctggt ggtgtttgtg gtgaactgaa ttgaatccat tgttgggctt
     7021 aagttactga aattggaaca ccctttgtcc ttctcggcgg gggcttcctg gtctgtgctt
     7081 tacttggctt ttttccttcc cgtcttagcc tcaccccctt gtcaaccaga ttgagttgct
     7141 atagcttgat gcagggaccc agtgaagttt ctccgttaaa gattgggagt cgtcgaaatg
     7201 tttagattct tttaggaaag gaattatttt cccccctttt acagggtagt aacttctcca
     7261 cagaagtgcc aatatggcaa aattacacaa gaaaacagta ttgcaatgac accattacat
     7321 aaggaacatt gaactgttag aggagtgctc ttccaaacaa aacaaaaatg tctctaggtt
     7381 tagtcagagc tttcacaagt aataaccttt ctgtattaaa atcagagtaa ccctttctgt
     7441 attgagtgca gtgtttttta ctcttttctc atgcacatgt tacgttggag aaaatgttta
     7501 caaaaatggt tttgttacac taatgcgcac cacatattta tggtttattt taagtgactt
     7561 tttatgggtt atttaggttt tcgtcttagt tgtagcacac ttaccctaat tttgccaatt
     7621 attaatttgc taaatagtaa tacaaatgac aaactgcatt aaatttacta attataaaag
     7681 ctgcaaagca gactggtggc aagtacacag cccttttttt tgcagtgcta acttgtctac
     7741 tgtgtattat gaaaattact gttgtccccc cacccttttt tccttaaata aagtaaaaat
     7801 gacaccta
//
