LOCUS       NM_003618               4335 bp    mRNA    linear   PRI 19-SEP-2024
DEFINITION  Homo sapiens mitogen-activated protein kinase kinase kinase kinase
            3 (MAP4K3), transcript variant 1, mRNA.
ACCESSION   NM_003618
VERSION     NM_003618.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4335)
  AUTHORS   Chuang,H.C., Hung,W.T., Chen,Y.M., Hsu,P.M., Yen,J.H., Lan,J.L. and
            Tan,T.H.
  TITLE     Genomic sequencing and functional analyses identify MAP4K3/GLK
            germline and somatic variants associated with systemic lupus
            erythematosus
  JOURNAL   Ann Rheum Dis 81 (2), 243-254 (2022)
   PUBMED   34610951
  REMARK    GeneRIF: Genomic sequencing and functional analyses identify
            MAP4K3/GLK germline and somatic variants associated with systemic
            lupus erythematosus.
REFERENCE   2  (bases 1 to 4335)
  AUTHORS   Song,Y., Zhang,F., Li,L., Song,B., Xu,J., Wang,G. and Zheng,Z.
  TITLE     MiR-338-3p inhibits growth of glioblastoma through targeting MAP4K3
  JOURNAL   Minerva Med 112 (4), 531-533 (2021)
   PUBMED   31578840
  REMARK    GeneRIF: MiR-338-3p inhibits growth of glioblastoma through
            targeting MAP4K3.
REFERENCE   3  (bases 1 to 4335)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   4  (bases 1 to 4335)
  AUTHORS   Buljan,M., Ciuffa,R., van Drogen,A., Vichalkovski,A., Mehnert,M.,
            Rosenberger,G., Lee,S., Varjosalo,M., Pernas,L.E., Spegg,V.,
            Snijder,B., Aebersold,R. and Gstaiger,M.
  TITLE     Kinase Interaction Network Expands Functional and Disease Roles of
            Human Kinases
  JOURNAL   Mol Cell 79 (3), 504-520 (2020)
   PUBMED   32707033
REFERENCE   5  (bases 1 to 4335)
  AUTHORS   Chuang,H.C., Chang,C.C., Teng,C.F., Hsueh,C.H., Chiu,L.L.,
            Hsu,P.M., Lee,M.C., Hsu,C.P., Chen,Y.R., Liu,Y.C., Lyu,P.C. and
            Tan,T.H.
  TITLE     MAP4K3/GLK Promotes Lung Cancer Metastasis by Phosphorylating and
            Activating IQGAP1
  JOURNAL   Cancer Res 79 (19), 4978-4993 (2019)
   PUBMED   31431460
  REMARK    GeneRIF: These findings show the critical role of the GLK-IQGAP
            cascade in cell migration and tumor metastasis
REFERENCE   6  (bases 1 to 4335)
  AUTHORS   Bandyopadhyay,S., Chiang,C.Y., Srivastava,J., Gersten,M., White,S.,
            Bell,R., Kurschner,C., Martin,C., Smoot,M., Sahasrabudhe,S.,
            Barber,D.L., Chanda,S.K. and Ideker,T.
  TITLE     A human MAP kinase interactome
  JOURNAL   Nat Methods 7 (10), 801-805 (2010)
   PUBMED   20936779
REFERENCE   7  (bases 1 to 4335)
  AUTHORS   Yan,L., Mieulet,V., Burgess,D., Findlay,G.M., Sully,K., Procter,J.,
            Goris,J., Janssens,V., Morrice,N.A. and Lamb,R.F.
  TITLE     PP2A T61 epsilon is an inhibitor of MAP4K3 in nutrient signaling to
            mTOR
  JOURNAL   Mol Cell 37 (5), 633-642 (2010)
   PUBMED   20227368
  REMARK    GeneRIF: Amino acid sufficiency phosphorylates MAP4K3/Ser170,
            activating mTORC1, but amino acid restriction causes MAP4K3 to
            interact with PP2A(T61 epsilon), promoting dephosphorylation of
            Ser170, MAP4K3 inhibition, and, inhibition of mTORC1 signaling.
REFERENCE   8  (bases 1 to 4335)
  AUTHORS   Lam,D. and Martins,L.M.
  TITLE     MAP4K3 enhances the expression of the BH3-only protein BID
  JOURNAL   Cell Cycle 8 (20), 3248-3249 (2009)
   PUBMED   19806019
REFERENCE   9  (bases 1 to 4335)
  AUTHORS   Lam,D., Dickens,D., Reid,E.B., Loh,S.H., Moisoi,N. and Martins,L.M.
  TITLE     MAP4K3 modulates cell death via the post-transcriptional regulation
            of BH3-only proteins
  JOURNAL   Proc Natl Acad Sci U S A 106 (29), 11978-11983 (2009)
   PUBMED   19587239
  REMARK    GeneRIF: MAP4K3 orchestrates activation of BAX via the concerted
            posttranscriptional modulation of PUMA, BAD, and BIM.
REFERENCE   10 (bases 1 to 4335)
  AUTHORS   Diener,K., Wang,X.S., Chen,C., Meyer,C.F., Keesler,G., Zukowski,M.,
            Tan,T.H. and Yao,Z.
  TITLE     Activation of the c-Jun N-terminal kinase pathway by a novel
            protein kinase related to human germinal center kinase
  JOURNAL   Proc Natl Acad Sci U S A 94 (18), 9687-9692 (1997)
   PUBMED   9275185
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DA409370.1, AK291467.1,
            AC007684.3, AK021553.1 and AC079615.6.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_003618.3.
            
            Summary: This gene encodes a member of the mitogen-activated
            protein kinase kinase kinase kinase family. The encoded protein
            activates key effectors in cell signalling, among them c-Jun.
            Alternatively spliced transcripts encoding multiple isoforms have
            been observed for this gene. [provided by RefSeq, Jul 2012].
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer protein (isoform 1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC060880.1, SRR1660809.155177.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000263881.8/ ENSP00000263881.3
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-7                 DA409370.1         30-36
            8-1049              AK291467.1         1-1042
            1050-1050           AC007684.3         133040-133040
            1051-3400           AK291467.1         1044-3393
            3401-3774           AK021553.1         932-1305
            3775-4335           AC079615.6         14151-14711
FEATURES             Location/Qualifiers
     source          1..4335
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="2"
                     /map="2p22.1"
     gene            1..4335
                     /gene="MAP4K3"
                     /gene_synonym="GLK; MAPKKKK3; MEKKK 3; MEKKK3; RAB8IPL1"
                     /note="mitogen-activated protein kinase kinase kinase
                     kinase 3"
                     /db_xref="GeneID:8491"
                     /db_xref="HGNC:HGNC:6865"
                     /db_xref="MIM:604921"
     exon            1..394
                     /gene="MAP4K3"
                     /gene_synonym="GLK; MAPKKKK3; MEKKK 3; MEKKK3; RAB8IPL1"
                     /inference="alignment:Splign:2.1.0"
     CDS             299..2983
                     /gene="MAP4K3"
                     /gene_synonym="GLK; MAPKKKK3; MEKKK 3; MEKKK3; RAB8IPL1"
                     /EC_number="2.7.11.1"
                     /note="isoform 1 is encoded by transcript variant 1;
                     germinal center kinase-related protein kinase; germinal
                     center kinase-like kinase; MEK kinase kinase 3; MAPK/ERK
                     kinase kinase kinase 3"
                     /codon_start=1
                     /product="mitogen-activated protein kinase kinase kinase
                     kinase 3 isoform 1"
                     /protein_id="NP_003609.2"
                     /db_xref="CCDS:CCDS1803.1"
                     /db_xref="GeneID:8491"
                     /db_xref="HGNC:HGNC:6865"
                     /db_xref="MIM:604921"
                     /translation="MNPGFDLSRRNPQEDFELIQRIGSGTYGDVYKARNVNTGELAAI
                     KVIKLEPGEDFAVVQQEIIMMKDCKHPNIVAYFGSYLRRDKLWICMEFCGGGSLQDIY
                     HVTGPLSELQIAYVSRETLQGLYYLHSKGKMHRDIKGANILLTDNGHVKLADFGVSAQ
                     ITATIAKRKSFIGTPYWMAPEVAAVERKGGYNQLCDLWAVGITAIELAELQPPMFDLH
                     PMRALFLMTKSNFQPPKLKDKMKWSNSFHHFVKMALTKNPKKRPTAEKLLQHPFVTQH
                     LTRSLAIELLDKVNNPDHSTYHDFDDDDPEPLVAVPHRIHSTSRNVREEKTRSEITFG
                     QVKFDPPLRKETEPHHELPDSDGFLDSSEEIYYTARSNLDLQLEYGQGHQGGYFLGAN
                     KSLLKSVEEELHQRGHVAHLEDDEGDDDESKHSTLKAKIPPPLPPKPKSIFIPQEMHS
                     TEDENQGTIKRCPMSGSPAKPSQVPPRPPPPRLPPHKPVALGNGMSSFQLNGERDGSL
                     CQQQNEHRGTNLSRKEKKDVPKPISNGLPPTPKVHMGACFSKVFNGCPLKIHCASSWI
                     NPDTRDQYLIFGAEEGIYTLNLNELHETSMEQLFPRRCTWLYVMNNCLLSISGKASQL
                     YSHNLPGLFDYARQMQKLPVAIPAHKLPDRILPRKFSVSAKIPETKWCQKCCVVRNPY
                     TGHKYLCGALQTSIVLLEWVEPMQKFMLIKHIDFPIPCPLRMFEMLVVPEQEYPLVCV
                     GVSRGRDFNQVVRFETVNPNSTSSWFTESDTPQTNVTHVTQLERDTILVCLDCCIKIV
                     NLQGRLKSSRKLSSELTFDFQIESIVCLQDSVLAFWKHGMQGRSFRSNEVTQEISDST
                     RIFRLLGSDRVVVLESRPTDNPTANSNLYILAGHENSY"
     exon            395..452
                     /gene="MAP4K3"
                     /gene_synonym="GLK; MAPKKKK3; MEKKK 3; MEKKK3; RAB8IPL1"
                     /inference="alignment:Splign:2.1.0"
     exon            453..543
                     /gene="MAP4K3"
                     /gene_synonym="GLK; MAPKKKK3; MEKKK 3; MEKKK3; RAB8IPL1"
                     /inference="alignment:Splign:2.1.0"
     exon            544..608
                     /gene="MAP4K3"
                     /gene_synonym="GLK; MAPKKKK3; MEKKK 3; MEKKK3; RAB8IPL1"
                     /inference="alignment:Splign:2.1.0"
     exon            609..664
                     /gene="MAP4K3"
                     /gene_synonym="GLK; MAPKKKK3; MEKKK 3; MEKKK3; RAB8IPL1"
                     /inference="alignment:Splign:2.1.0"
     exon            665..712
                     /gene="MAP4K3"
                     /gene_synonym="GLK; MAPKKKK3; MEKKK 3; MEKKK3; RAB8IPL1"
                     /inference="alignment:Splign:2.1.0"
     exon            713..755
                     /gene="MAP4K3"
                     /gene_synonym="GLK; MAPKKKK3; MEKKK 3; MEKKK3; RAB8IPL1"
                     /inference="alignment:Splign:2.1.0"
     exon            756..828
                     /gene="MAP4K3"
                     /gene_synonym="GLK; MAPKKKK3; MEKKK 3; MEKKK3; RAB8IPL1"
                     /inference="alignment:Splign:2.1.0"
     exon            829..960
                     /gene="MAP4K3"
                     /gene_synonym="GLK; MAPKKKK3; MEKKK 3; MEKKK3; RAB8IPL1"
                     /inference="alignment:Splign:2.1.0"
     exon            961..1023
                     /gene="MAP4K3"
                     /gene_synonym="GLK; MAPKKKK3; MEKKK 3; MEKKK3; RAB8IPL1"
                     /inference="alignment:Splign:2.1.0"
     exon            1024..1105
                     /gene="MAP4K3"
                     /gene_synonym="GLK; MAPKKKK3; MEKKK 3; MEKKK3; RAB8IPL1"
                     /inference="alignment:Splign:2.1.0"
     exon            1106..1216
                     /gene="MAP4K3"
                     /gene_synonym="GLK; MAPKKKK3; MEKKK 3; MEKKK3; RAB8IPL1"
                     /inference="alignment:Splign:2.1.0"
     exon            1217..1295
                     /gene="MAP4K3"
                     /gene_synonym="GLK; MAPKKKK3; MEKKK 3; MEKKK3; RAB8IPL1"
                     /inference="alignment:Splign:2.1.0"
     exon            1296..1354
                     /gene="MAP4K3"
                     /gene_synonym="GLK; MAPKKKK3; MEKKK 3; MEKKK3; RAB8IPL1"
                     /inference="alignment:Splign:2.1.0"
     exon            1355..1417
                     /gene="MAP4K3"
                     /gene_synonym="GLK; MAPKKKK3; MEKKK 3; MEKKK3; RAB8IPL1"
                     /inference="alignment:Splign:2.1.0"
     exon            1418..1476
                     /gene="MAP4K3"
                     /gene_synonym="GLK; MAPKKKK3; MEKKK 3; MEKKK3; RAB8IPL1"
                     /inference="alignment:Splign:2.1.0"
     exon            1477..1515
                     /gene="MAP4K3"
                     /gene_synonym="GLK; MAPKKKK3; MEKKK 3; MEKKK3; RAB8IPL1"
                     /inference="alignment:Splign:2.1.0"
     exon            1516..1569
                     /gene="MAP4K3"
                     /gene_synonym="GLK; MAPKKKK3; MEKKK 3; MEKKK3; RAB8IPL1"
                     /inference="alignment:Splign:2.1.0"
     exon            1570..1612
                     /gene="MAP4K3"
                     /gene_synonym="GLK; MAPKKKK3; MEKKK 3; MEKKK3; RAB8IPL1"
                     /inference="alignment:Splign:2.1.0"
     exon            1613..1772
                     /gene="MAP4K3"
                     /gene_synonym="GLK; MAPKKKK3; MEKKK 3; MEKKK3; RAB8IPL1"
                     /inference="alignment:Splign:2.1.0"
     exon            1773..1885
                     /gene="MAP4K3"
                     /gene_synonym="GLK; MAPKKKK3; MEKKK 3; MEKKK3; RAB8IPL1"
                     /inference="alignment:Splign:2.1.0"
     exon            1886..1927
                     /gene="MAP4K3"
                     /gene_synonym="GLK; MAPKKKK3; MEKKK 3; MEKKK3; RAB8IPL1"
                     /inference="alignment:Splign:2.1.0"
     exon            1928..2012
                     /gene="MAP4K3"
                     /gene_synonym="GLK; MAPKKKK3; MEKKK 3; MEKKK3; RAB8IPL1"
                     /inference="alignment:Splign:2.1.0"
     exon            2013..2092
                     /gene="MAP4K3"
                     /gene_synonym="GLK; MAPKKKK3; MEKKK 3; MEKKK3; RAB8IPL1"
                     /inference="alignment:Splign:2.1.0"
     exon            2093..2153
                     /gene="MAP4K3"
                     /gene_synonym="GLK; MAPKKKK3; MEKKK 3; MEKKK3; RAB8IPL1"
                     /inference="alignment:Splign:2.1.0"
     exon            2154..2271
                     /gene="MAP4K3"
                     /gene_synonym="GLK; MAPKKKK3; MEKKK 3; MEKKK3; RAB8IPL1"
                     /inference="alignment:Splign:2.1.0"
     exon            2272..2330
                     /gene="MAP4K3"
                     /gene_synonym="GLK; MAPKKKK3; MEKKK 3; MEKKK3; RAB8IPL1"
                     /inference="alignment:Splign:2.1.0"
     exon            2331..2434
                     /gene="MAP4K3"
                     /gene_synonym="GLK; MAPKKKK3; MEKKK 3; MEKKK3; RAB8IPL1"
                     /inference="alignment:Splign:2.1.0"
     exon            2435..2606
                     /gene="MAP4K3"
                     /gene_synonym="GLK; MAPKKKK3; MEKKK 3; MEKKK3; RAB8IPL1"
                     /inference="alignment:Splign:2.1.0"
     exon            2607..2675
                     /gene="MAP4K3"
                     /gene_synonym="GLK; MAPKKKK3; MEKKK 3; MEKKK3; RAB8IPL1"
                     /inference="alignment:Splign:2.1.0"
     exon            2676..2768
                     /gene="MAP4K3"
                     /gene_synonym="GLK; MAPKKKK3; MEKKK 3; MEKKK3; RAB8IPL1"
                     /inference="alignment:Splign:2.1.0"
     exon            2769..2839
                     /gene="MAP4K3"
                     /gene_synonym="GLK; MAPKKKK3; MEKKK 3; MEKKK3; RAB8IPL1"
                     /inference="alignment:Splign:2.1.0"
     exon            2840..2895
                     /gene="MAP4K3"
                     /gene_synonym="GLK; MAPKKKK3; MEKKK 3; MEKKK3; RAB8IPL1"
                     /inference="alignment:Splign:2.1.0"
     exon            2896..4335
                     /gene="MAP4K3"
                     /gene_synonym="GLK; MAPKKKK3; MEKKK 3; MEKKK3; RAB8IPL1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4301..4306
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MAP4K3"
                     /gene_synonym="GLK; MAPKKKK3; MEKKK 3; MEKKK3; RAB8IPL1"
                     /note="hexamer: AATAAA"
     polyA_site      4335
                     /gene="MAP4K3"
                     /gene_synonym="GLK; MAPKKKK3; MEKKK 3; MEKKK3; RAB8IPL1"
                     /note="major polyA site"
ORIGIN      
        1 ggaagccatg ttggagtttc accccgagcg aggggctgcg agggttctcc tctccttgtg
       61 ggtgtgacca ggttcggcgc ggcggcgggc cgcggggggc ggggacgatt ggccgcttgt
      121 cgtcgtccgc agcggcgtga ggggagcggc ggcggcggcg gcggccgcag cgtggagccg
      181 ggtgattgtg accgccgggg gagccgggag ccggccgcgg cgccctctct ccgtgtggcc
      241 ccctgagcgg cccccctccc ctgcccggga gggaggcggg gggcacctgg ggcccgccat
      301 gaaccccggc ttcgatttgt cccgccggaa cccgcaggag gacttcgagc tgattcagcg
      361 catcggcagc ggcacctacg gcgacgtcta caaggcacgg aatgttaaca ctggtgaatt
      421 agcagcaatt aaagtaataa aattggaacc aggagaagac tttgcagttg tgcagcaaga
      481 aattattatg atgaaagact gtaaacaccc aaatattgtt gcttattttg gaagctatct
      541 caggcgagat aagctttgga tttgcatgga gttttgtgga ggtggttctt tacaggatat
      601 ttatcacgta actggacctc tgtcagaact gcaaattgca tatgttagca gagaaacact
      661 gcagggatta tattatcttc acagtaaagg aaaaatgcac agagatataa agggagctaa
      721 cattctatta acggataatg gtcatgtgaa attggctgat tttggagtat ctgcacagat
      781 aacagctaca attgccaaac ggaagtcttt cattggcaca ccatattgga tggctccaga
      841 agttgcagct gttgagagga aggggggtta caatcaactc tgtgatctct gggcagtggg
      901 aatcactgcc atagaacttg cagagcttca gcctcctatg tttgacttac acccaatgag
      961 agcattattt ctaatgacaa aaagcaattt tcagcctcct aaactaaagg ataaaatgaa
     1021 atggtcaaat agttttcatc actttgtgaa aatggcactt accaaaaatc cgaaaaaaag
     1081 acctactgct gaaaaattat tacagcatcc ttttgtaaca caacatttga cacggtcttt
     1141 ggcaatcgag ctgttggata aagtaaataa tccagatcat tccacttacc atgatttcga
     1201 tgatgatgat cctgagcctc ttgttgctgt accacataga attcactcaa caagtagaaa
     1261 cgtgagagaa gaaaaaacac gctcagagat aacctttggc caagtgaaat ttgatccacc
     1321 cttaagaaag gagacagaac cacatcatga acttcccgac agtgatggtt ttttggacag
     1381 ttcagaagaa atatactaca ctgcaagatc taatctggat ctgcaactgg aatatggaca
     1441 aggacaccaa ggtggttact ttttaggtgc aaacaagagt cttctcaagt ctgttgaaga
     1501 agaattgcat cagcgaggac acgtcgcaca tttagaagat gatgaaggag atgatgatga
     1561 atctaaacac tcaactctga aagcaaaaat tccacctcct ttgccaccaa agcctaagtc
     1621 tatcttcata ccacaggaaa tgcattctac tgaggatgaa aatcaaggaa caatcaagag
     1681 atgtcccatg tcagggagcc cagcaaagcc atcccaagtt ccacctagac caccacctcc
     1741 cagattaccc ccacacaaac ctgttgcctt aggaaatgga atgagctcct tccagttaaa
     1801 tggtgaacga gatggctcat tatgtcaaca acagaatgaa catagaggca caaacctttc
     1861 aagaaaagaa aagaaagatg taccaaagcc tattagtaat ggtcttcctc caacacctaa
     1921 agtgcatatg ggtgcatgtt tttcaaaagt ttttaatggg tgtcccttga aaattcactg
     1981 tgcatcatca tggataaacc cagatacaag agatcagtac ttgatatttg gtgccgaaga
     2041 agggatttat accctcaatc ttaatgaact tcatgaaaca tcaatggaac agctattccc
     2101 tcgaaggtgt acatggttgt atgtaatgaa caattgcttg ctatcaatat ctggtaaagc
     2161 ttctcagctt tattcccata atttaccagg gctttttgat tatgcaagac aaatgcaaaa
     2221 gttacctgtt gctattccag cacacaaact ccctgacaga atactgccaa ggaaattttc
     2281 tgtatcagca aaaatccctg aaaccaaatg gtgccagaag tgttgtgttg taagaaatcc
     2341 ttacacgggc cataaatacc tatgtggagc acttcagact agcattgttc tattagaatg
     2401 ggttgaacca atgcagaaat ttatgttaat taagcacata gattttccta taccatgtcc
     2461 acttagaatg tttgaaatgc tggtagttcc tgaacaggag taccctttag tttgtgttgg
     2521 tgtcagtaga ggtagagact tcaaccaagt ggttcgattt gagacggtca atccaaattc
     2581 tacctcttca tggtttacag aatcagatac cccacagaca aatgttactc atgtaaccca
     2641 actggagaga gataccatcc ttgtatgctt ggactgttgt ataaaaatag taaatctcca
     2701 aggaagatta aaatctagca ggaaattgtc atcagaactc acctttgatt tccagattga
     2761 atcaatagtg tgcctacaag acagtgtgct agctttctgg aaacatggaa tgcaaggtag
     2821 aagttttaga tctaatgagg taacacaaga aatttcagat agcacaagaa ttttcaggct
     2881 gcttggatct gacagggtcg tggttttgga aagtaggcca actgataacc ccacagcaaa
     2941 tagcaatttg tacatcctgg cgggtcatga aaacagttac tgagaattgt tgtgctttga
     3001 cagttaactc tagaaagaaa gaacactacc actgcaacat taatggatgc ttgaagctgt
     3061 acaaaagctg cagtaacctg tcttcagtta ctttgtaatt tattgtggca tgagataaga
     3121 tggggaaaat tttgttttaa gtggtatgga tatatttagc atattgaacc acacaagtgc
     3181 ttaattcatt gttatgtaat ctttgtacat ataggcagta ttttttctgt gaaacttcat
     3241 attgctgaag acatacacta agaatttatg tagataatgt acttttatga gatgtacaag
     3301 taagtgtctt atctgtacag atgtaaatgt tgatgaaaat gcaattgggg ttaatatttt
     3361 aagaattctt tagtatattc ttgggtgtgg ctatattaca aaatgggatg ctggcaatga
     3421 aacaatacat ttaacactat tgtattttta ttatatgtaa tttagtaata tgaatataaa
     3481 tcttgtaact tttaaaattg taatggaggc tgtaatcatt ttataatctt tttaatttta
     3541 atgcaagtac actggtgttt atatttgcac aaagtattga tatgtgatgt attaagtcac
     3601 aaaagtaagc tgtgacattg tctataagca tttggctcca caaatgtatt tggattgttt
     3661 tctatgtgaa gcaaaccaat tataattaac cacatgttgt agtaactggt ctttttatat
     3721 ttaagcagaa tcctgtaaga ttgcttgtct ttgcttaaaa acaatacctt tgaacatttt
     3781 tgaatcacag aatagcggta ccatgataga atactgcaat tgtggtcaga attacagtat
     3841 gcacaaagaa ttaattagca ttattaaaga gtcctcacta aacatttcat atgatcacac
     3901 tgaagaactg taacattcca tagagtgaag tggttcaaat ttctcttgga atttttactt
     3961 ttgttggcct tattttatga tccttttcat atttcttttg acttagagta ttaatacatg
     4021 gccaaaataa tttagttact acctcataca aacaatataa tggttactac acatcacagg
     4081 aacttagttt tggtttaagt catttttgat tgcttttttc caatggaata tgtatatacc
     4141 aggttttagc aaaatgcaca cttttggctc tttttggtat atgttcttta tattttaatg
     4201 tgagtatata cactaagaac aaactaaatt gtgatttatg atcttcattt attttaatga
     4261 taatggtttt aaaatatgtt cctgattgta catattgtaa aataaacatg ttttttaaca
     4321 tgctgttgta tagta
//
