LOCUS       NM_001385682            9232 bp    mRNA    linear   PRI 21-SEP-2024
DEFINITION  Homo sapiens microtubule associated protein 4 (MAP4), transcript
            variant 134, mRNA.
ACCESSION   NM_001385682
VERSION     NM_001385682.1
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 9232)
  AUTHORS   Thapa,N., Chen,M., Cryns,V.L. and Anderson,R.
  TITLE     A p85 isoform switch enhances PI3K activation on endosomes by a
            MAP4- and PI3P-dependent mechanism
  JOURNAL   Cell Rep 43 (5), 114119 (2024)
   PUBMED   38630589
  REMARK    GeneRIF: A p85 isoform switch enhances PI3K activation on endosomes
            by a MAP4- and PI3P-dependent mechanism.
REFERENCE   2  (bases 1 to 9232)
  AUTHORS   Xia,X., Ge,Y., Ge,F., Gu,P., Liu,Y., Li,P. and Xu,P.
  TITLE     MAP4 acts as an oncogene and prognostic marker and affects
            radioresistance by mediating epithelial-mesenchymal transition in
            lung adenocarcinoma
  JOURNAL   J Cancer Res Clin Oncol 150 (2), 88 (2024)
   PUBMED   38341398
  REMARK    GeneRIF: MAP4 acts as an oncogene and prognostic marker and affects
            radioresistance by mediating epithelial-mesenchymal transition in
            lung adenocarcinoma.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 9232)
  AUTHORS   Nishida,K., Matsumura,K., Tamura,M., Nakamichi,T., Shimamori,K.,
            Kuragano,M., Kabir,A.M.R., Kakugo,A., Kotani,S., Nishishita,N. and
            Tokuraku,K.
  TITLE     Effects of three microtubule-associated proteins (MAP2, MAP4, and
            Tau) on microtubules' physical properties and neurite morphology
  JOURNAL   Sci Rep 13 (1), 8870 (2023)
   PUBMED   37258650
  REMARK    GeneRIF: Effects of three microtubule-associated proteins (MAP2,
            MAP4, and Tau) on microtubules' physical properties and neurite
            morphology.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 9232)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   5  (bases 1 to 9232)
  AUTHORS   Wang,L., Paudyal,S.C., Kang,Y., Owa,M., Liang,F.X., Spektor,A.,
            Knaut,H., Sanchez,I. and Dynlacht,B.D.
  TITLE     Regulators of tubulin polyglutamylation control nuclear shape and
            cilium disassembly by balancing microtubule and actin assembly
  JOURNAL   Cell Res 32 (2), 190-209 (2022)
   PUBMED   34782749
REFERENCE   6  (bases 1 to 9232)
  AUTHORS   Ookata,K., Hisanaga,S., Bulinski,J.C., Murofushi,H., Aizawa,H.,
            Itoh,T.J., Hotani,H., Okumura,E., Tachibana,K. and Kishimoto,T.
  TITLE     Cyclin B interaction with microtubule-associated protein 4 (MAP4)
            targets p34cdc2 kinase to microtubules and is a potential regulator
            of M-phase microtubule dynamics
  JOURNAL   J Cell Biol 128 (5), 849-862 (1995)
   PUBMED   7876309
REFERENCE   7  (bases 1 to 9232)
  AUTHORS   Chapin,S.J., Lue,C.M., Yu,M.T. and Bulinski,J.C.
  TITLE     Differential expression of alternatively spliced forms of MAP4: a
            repertoire of structurally different microtubule-binding domains
  JOURNAL   Biochemistry 34 (7), 2289-2301 (1995)
   PUBMED   7857940
REFERENCE   8  (bases 1 to 9232)
  AUTHORS   Chapin,S.J. and Bulinski,J.C.
  TITLE     Microtubule stabilization by assembly-promoting
            microtubule-associated proteins: a repeat performance
  JOURNAL   Cell Motil Cytoskeleton 23 (4), 236-243 (1992)
   PUBMED   1477887
  REMARK    Review article
REFERENCE   9  (bases 1 to 9232)
  AUTHORS   West,R.R., Tenbarge,K.M. and Olmsted,J.B.
  TITLE     A model for microtubule-associated protein 4 structure. Domains
            defined by comparisons of human, mouse, and bovine sequences
  JOURNAL   J Biol Chem 266 (32), 21886-21896 (1991)
   PUBMED   1718985
REFERENCE   10 (bases 1 to 9232)
  AUTHORS   Chapin,S.J. and Bulinski,J.C.
  TITLE     Non-neuronal 210 x 10(3) Mr microtubule-associated protein (MAP4)
            contains a domain homologous to the microtubule-binding domains of
            neuronal MAP2 and tau
  JOURNAL   J Cell Sci 98 (Pt 1), 27-36 (1991)
   PUBMED   1905296
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC124916.3 and AC139667.5.
            This sequence is a reference standard in the RefSeqGene project.
            
            Summary: The protein encoded by this gene is a major non-neuronal
            microtubule-associated protein. This protein contains a domain
            similar to the microtubule-binding domains of neuronal
            microtubule-associated protein (MAP2) and microtubule-associated
            protein tau (MAPT/TAU). This protein promotes microtubule assembly,
            and has been shown to counteract destabilization of interphase
            microtubule catastrophe promotion. Cyclin B was found to interact
            with this protein, which targets cell division cycle 2 (CDC2)
            kinase to microtubules. The phosphorylation of this protein affects
            microtubule properties and cell cycle progression. Multiple
            transcript variants encoding different isoforms have been found for
            this gene. [provided by RefSeq, Aug 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            RNAseq introns :: mixed sample support SAMEA1965299, SAMEA1966682
                              [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000683076.1/ ENSP00000507895.1
            RefSeq Select criteria :: based on manual assertion, conservation
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-83                AC124916.3         41130-41212         c
            84-325              AC124916.3         23434-23675         c
            326-394             AC124916.3         2661-2729           c
            395-517             AC139667.5         48903-49025
            518-631             AC139667.5         55375-55488
            632-754             AC139667.5         58412-58534
            755-1978            AC139667.5         60079-61302
            1979-2101           AC139667.5         62314-62436
            2102-5485           AC139667.5         64832-68215
            5486-5536           AC139667.5         74253-74303
            5537-5643           AC139667.5         99730-99836
            5644-5859           AC139667.5         101353-101568
            5860-6043           AC139667.5         105153-105336
            6044-6103           AC139667.5         105967-106026
            6104-6396           AC139667.5         106148-106440
            6397-6510           AC139667.5         107926-108039
            6511-6603           AC139667.5         109915-110007
            6604-6685           AC139667.5         119741-119822
            6686-6798           AC139667.5         121893-122005
            6799-6988           AC139667.5         123901-124090
            6989-9232           AC139667.5         124315-126558
FEATURES             Location/Qualifiers
     source          1..9232
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="3"
                     /map="3p21.31"
     gene            1..9232
                     /gene="MAP4"
                     /note="microtubule associated protein 4"
                     /db_xref="GeneID:4134"
                     /db_xref="HGNC:HGNC:6862"
                     /db_xref="MIM:157132"
     exon            1..83
                     /gene="MAP4"
                     /inference="alignment:Splign:2.1.0"
     exon            84..325
                     /gene="MAP4"
                     /inference="alignment:Splign:2.1.0"
     CDS             103..6993
                     /gene="MAP4"
                     /note="isoform 6 is encoded by transcript variant 134;
                     MAP-4"
                     /codon_start=1
                     /product="microtubule-associated protein 4 isoform 6"
                     /protein_id="NP_001372611.1"
                     /db_xref="CCDS:CCDS93263.1"
                     /db_xref="GeneID:4134"
                     /db_xref="HGNC:HGNC:6862"
                     /db_xref="MIM:157132"
                     /translation="MADLSLADALTEPSPDIEGEIKRDFIATLEAEAFDDVVGETVGK
                     TDYIPLLDVDEKTGNSESKKKPCSETSQIEDTPSSKPTLLANGGHGVEGSDTTGSPTE
                     FLEEKMAYQEYPNSQNWPEDTNFCFQPEQVVDPIQTDPFKMYHDDDLADLVFPSSATA
                     DTSIFAGQNDPLKDSYGMSPCNTAVVPQGWSVEALNSPHSESFVSPEAVAEPPQPTAV
                     PLELAKEIEMASEERPPAQALEIMMGLKTTDMAPSKETEMALAKDMALATKTEVALAK
                     DMESPTKLDVTLAKDMQPSMESDMALVKDMELPTEKEVALVKDVRWPTETDVSSAKNV
                     VLPTETEVAPAKDVTLLKETERASPIKMDLAPSKDMGPPKENKKETERASPIKMDLAP
                     SKDMGPPKENKIVPAKDLVLLSEIEVAQANDIISSTEISSAEKVALSSETEVALARDM
                     TLPPETNVILTKDKALPLEAEVAPVKDMAQLPETEIAPAKDVAPSTVKEVGLLKDMSP
                     LSETEMALGKDVTPPPETEVVLIKNVCLPPEMEVALTEDQVPALKTEAPLAKDGVLTL
                     ANNVTPAKDVPPLSETEATPVPIKDMEIAQTQKGISEDSHLESLQDVGQSAAPTFMIS
                     PETVTGTGKKCSLPAEEDSVLEKLGERKPCNSQPSELSSETSANFMYCGTPPTQAKQV
                     CRPSDRRSTRPKPARVPPELLGGSPPWKTLDHRLGHCSLSESGWVSGSSSCGGPGNQR
                     KSIHVDSLEPQRDLGREAWDIESTPIMMKKKKKKPKQKRYSQPRAGGPSDDDNADKPK
                     GHPFAADTQKSGVLPSQPTTMGTEYGLVSGENLKRECLVNSSAARLVAENFVSESLRI
                     PLYPSEEAPKTAISSQSKLRVEEESKSNKSVLQNQDKKLLKQHEYKPQPAPHLKTPVD
                     KSQSVGPLNLKGPLAEVSAYNVETPLDIRLKEGCSPFLDQEVMGVVSKPTAAKEIPNL
                     VPTLIASNPLECNLKEGNNESKMTKLQNVKLKEFPEGAEEDKELKKEAFPNERQEISI
                     FTSEQLQGQVLVQVPGVENEPFKRMAGDGKSRKGRGSSGKMRTDSGKVKAKSELPFLL
                     DSQKDGRAVLIPSEPVSKTEGMTTQDKSEELGLNSSKQPGTKADLTEAVVMGEPKEMT
                     QPKVAGTMQALIPLESGSGMTQTSGVSTETGDVVKDMGVNNQSKEGRCPWKDHEAAPW
                     ISEKPKKRGNEGKSKKFKNNYSTQPARMERKEEILNPPFEGKDGDTGSIPHKSKEIGF
                     TFPKMHDSSFSHTPDTPTVEAVDRKGGNFQVNFVELGTLGENKISTVKASTVTEPPAK
                     VTDVSCQEQIQGAGFVPSVVSEENKTDAANRYTAVADKPSKRSNDGKSKKVKNSSPEK
                     HILENKIDATKIHVPMETTGDQGIEGMAYMDENRNITFTCPRTPSELINKSSPLEVLE
                     SAACEKLPTPTPQVVKEGDSFPDTLAKNGQEIAPAQISKSLMVDNYTKDGVPGQERPK
                     GPSAVVPSTSTGGVALPITTAIETVNIHGDHSLKNKAELADSMKNEAGIDEGHVIGES
                     ESVHSGASKHSVEKVTELAKGHLLPGVPVEDQSLPGEARALEGYADRGNFPAHPVNEE
                     KETKEGSVAVQIPDLLEDKAQKLSFCEDQNAQDRNSKGSDSLNKKVDLTLLSPKSEND
                     KLKEISLACKITELESVSLPTPEIQSDFLHSKVEAPPSEVADTLVIMTASKGVRLPEP
                     KDKILETPQKMTEKSESKTPGEGKKEDKSRMAEPMKGYMRPTKSRGLTPLLPKSTIQE
                     QERHKQLKSAVCLSSSTVYQQLGMSVYGIARPEEGRPVVSGTGNDITTPPNKELPPSP
                     EKKTKPLATTQPAKTSTSKAKTQPTSLPKQPAPTTIGGLNKKPMSLASGLVPAAPPKR
                     PAVASARPSILPSKDVKPKPIADAKAPEKRASPSKPASAPASRSGSKSTQTVAKTTTA
                     AAVASTGPSSRSPSTLLPKKPTAIKTEGKPAEVKKMTAKSVPADLSRPKSTSTSSMKK
                     TTTLSGTAPAAGVVPSRVKATPMPSRPSTTPFIDKKPTSAKPSSTTPRLSRLATNTSA
                     PDLKNVRSKVGSTENIKHQPGGGRAKVEKKTEAAATTRKPESNAVTKTAGPIASAQKQ
                     PAGKVQIVSKKVSYSHIQSKCGSKDNIKHVPGGGNVQIQNKKVDISKVSSKCGSKANI
                     KHKPGGGDVKIESQKLNFKEKAQAKVGSLDNVGHLPAGGAVKTEGGGSEAPLCPGPPA
                     GEEPAISEAAPEAGAPTSASGLNGHPTLSGGGDQREAQTLDSQIQETN"
     exon            326..394
                     /gene="MAP4"
                     /inference="alignment:Splign:2.1.0"
     exon            395..517
                     /gene="MAP4"
                     /inference="alignment:Splign:2.1.0"
     exon            518..631
                     /gene="MAP4"
                     /inference="alignment:Splign:2.1.0"
     exon            632..754
                     /gene="MAP4"
                     /inference="alignment:Splign:2.1.0"
     exon            755..1978
                     /gene="MAP4"
                     /inference="alignment:Splign:2.1.0"
     exon            1979..2101
                     /gene="MAP4"
                     /inference="alignment:Splign:2.1.0"
     exon            2102..5485
                     /gene="MAP4"
                     /inference="alignment:Splign:2.1.0"
     exon            5486..5536
                     /gene="MAP4"
                     /inference="alignment:Splign:2.1.0"
     exon            5537..5643
                     /gene="MAP4"
                     /inference="alignment:Splign:2.1.0"
     exon            5644..5859
                     /gene="MAP4"
                     /inference="alignment:Splign:2.1.0"
     exon            5860..6043
                     /gene="MAP4"
                     /inference="alignment:Splign:2.1.0"
     exon            6044..6103
                     /gene="MAP4"
                     /inference="alignment:Splign:2.1.0"
     exon            6104..6396
                     /gene="MAP4"
                     /inference="alignment:Splign:2.1.0"
     exon            6397..6510
                     /gene="MAP4"
                     /inference="alignment:Splign:2.1.0"
     exon            6511..6603
                     /gene="MAP4"
                     /inference="alignment:Splign:2.1.0"
     exon            6604..6685
                     /gene="MAP4"
                     /inference="alignment:Splign:2.1.0"
     exon            6686..6798
                     /gene="MAP4"
                     /inference="alignment:Splign:2.1.0"
     exon            6799..6988
                     /gene="MAP4"
                     /inference="alignment:Splign:2.1.0"
     exon            6989..9232
                     /gene="MAP4"
                     /inference="alignment:Splign:2.1.0"
     regulatory      8350..8355
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MAP4"
                     /note="hexamer: AATAAA"
     polyA_site      8369
                     /gene="MAP4"
                     /note="major polyA site"
     regulatory      9208..9213
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MAP4"
                     /note="hexamer: AATAAA"
     polyA_site      9232
                     /gene="MAP4"
                     /note="major polyA site"
ORIGIN      
        1 atttggacag aaatgttgaa ggcaggtgcc tgatccaaaa tgcatttagt caggactgaa
       61 cacaagctgc ctggatagtt cctgcagttg cagtggtgca gaatggctga cctcagtctt
      121 gcagatgcat taacagaacc atctccagac attgagggag agataaagcg ggacttcatt
      181 gccacactag aggcagaggc ctttgatgat gttgtgggag aaactgttgg aaaaacagac
      241 tatattcctc tcctggatgt tgatgagaaa accgggaact cagagtcaaa gaagaaaccg
      301 tgctcagaaa ctagccagat tgaagatact ccatcttcta aaccaacact cctagccaat
      361 ggtggtcatg gagtagaagg gagcgatact acagggtctc caactgaatt ccttgaagag
      421 aaaatggcct accaggaata cccaaatagc cagaactggc cagaagatac caacttttgt
      481 ttccaacctg agcaagtggt cgatcctatc cagactgatc cctttaagat gtaccatgat
      541 gatgacctgg cagatttggt ctttccctcc agtgcgacag ctgatacttc aatatttgca
      601 ggacaaaatg atcccttgaa agacagttac ggtatgtctc cctgcaacac agctgttgta
      661 cctcaggggt ggtctgtgga agccttaaac tctccacact cagagtcctt tgtttcccca
      721 gaggctgttg cagaacctcc tcagccaacg gcagttccct tagagctagc caaggagata
      781 gaaatggcat cagaagagag gccaccagca caagcattgg aaataatgat gggactgaag
      841 actactgaca tggcaccatc taaagaaaca gagatggccc tcgccaagga catggcacta
      901 gctacaaaaa ccgaggtggc attggctaaa gatatggaat cacccaccaa attagatgtg
      961 acactggcca aggacatgca gccatccatg gaatcagata tggccctagt caaggacatg
     1021 gaactaccca cagaaaaaga agtggccctg gttaaggatg tcagatggcc cacagaaaca
     1081 gatgtatctt cagccaagaa tgtggtactg cccacagaaa cagaggtagc cccagccaag
     1141 gatgtgacac tgttgaaaga aacagagagg gcatctccta taaaaatgga cttagcccct
     1201 tccaaggaca tgggaccacc caaagaaaac aagaaagaaa cagagagggc atctcctata
     1261 aaaatggact tggctccttc caaggacatg ggaccaccca aagaaaacaa gatagtccca
     1321 gccaaggatt tggtattact ctcagaaata gaggtggcac aggctaatga cattatatca
     1381 tccacagaaa tatcctctgc tgagaaggtg gctttgtcct cagaaacaga ggtagccctg
     1441 gccagggaca tgacactgcc cccggaaacc aacgtgatct tgaccaagga taaagcacta
     1501 cctttagaag cagaggtggc cccagtcaag gacatggctc aactcccaga aacagaaata
     1561 gccccggcca aggatgtggc tccgtccaca gtaaaagaag tgggcttgtt gaaggacatg
     1621 tctccactat cagaaacaga aatggctctg ggcaaggatg tgactccacc tccagaaaca
     1681 gaagtagttc tcatcaagaa cgtatgtctg cctccagaaa tggaggtggc cctgactgag
     1741 gatcaggtcc cagccctcaa aacagaagca cccctggcta aggatggggt tctgaccctg
     1801 gccaacaatg tgactccagc caaagatgtt ccaccactct cagaaacaga ggcaacacca
     1861 gttccaatta aagacatgga aattgcacaa acacaaaaag gaataagtga ggattcccat
     1921 ttagaatctc tgcaggatgt ggggcagtca gctgcaccta ctttcatgat ttcaccagaa
     1981 accgtcacag gaacggggaa aaagtgcagc ttgccggccg aggaggattc tgtgttagaa
     2041 aaactagggg aaaggaaacc atgcaacagt caaccttctg agctttcttc agagacctca
     2101 gccaacttca tgtattgcgg tacccctccc acacaagcca aacaagtttg cagacccagt
     2161 gaccgcaggt caacccggcc caagcctgcc agggtccctc ctgagctgct gggaggctct
     2221 cctccatgga agactcttga tcacaggctg ggccactgct ctttgtctga gtcaggttgg
     2281 gtctctggtt catcctcctg tggtgggcct gggaaccaaa gaaaaagtat tcatgttgac
     2341 tcacttgaac cccagaggga tcttggcagg gaggcctggg atatagaaag cacaccaata
     2401 atgatgaaga aaaagaagaa gaaaccaaag caaaagagat attctcaacc acgggctgga
     2461 ggaccttcgg atgatgacaa tgcagataag cctaaaggtc atccatttgc agctgacaca
     2521 caaaaatcag gtgttctccc cagccagcct accactatgg gtacagaata tggacttgta
     2581 tctggagaaa acttgaaaag ggaatgttta gttaactcca gtgcagccag actggtagct
     2641 gagaactttg tctcagagag tctcagaatt cctttatatc cttctgaaga agcccccaaa
     2701 actgcaataa gttctcagtc taagctgaga gtagaggaag agagcaaaag taacaagtca
     2761 gtactacaaa accaagacaa gaaattgctg aagcaacatg aatacaaacc acagcctgca
     2821 ccccacctga agactcctgt agataaaagc cagtcagtag gccctctcaa tctgaaagga
     2881 cccctagcag aagtttctgc atacaatgta gaaacccctt tggatatcag acttaaagag
     2941 ggttgctctc ctttcttgga ccaagaggtt atgggtgtag tttcaaaacc cacagcagca
     3001 aaagaaatac caaatttggt acccactttg atagcaagta atccattaga atgtaatcta
     3061 aaagaaggga ataatgaaag taaaatgact aaactgcaga atgtcaaact gaaggagttt
     3121 cctgaaggag ctgaagagga taaagaacta aaaaaggaag cttttcccaa cgaaagacaa
     3181 gagatcagca tctttacttc tgagcagctg cagggccaag tgttggtaca ggtccctggg
     3241 gtagagaatg aaccatttaa gagaatggca ggtgatggca aaagcaggaa gggaagggga
     3301 agttctggga aaatgagaac agattctggg aaggtaaaag caaaatctga gctgccattt
     3361 cttctggaca gccagaagga cggaagggct gttctcatac cgagtgagcc agtctctaaa
     3421 actgaaggaa tgactactca ggataagagt gaggagctgg ggctgaattc ttcaaagcaa
     3481 ccaggcacta aggctgatct cacggaggca gtggtgatgg gggagcctaa agagatgact
     3541 cagcctaagg tggcaggcac catgcaggca ttgattcctt tggaaagtgg atcaggcatg
     3601 actcagactt ctggtgttag cacagaaaca ggagatgtag tcaaagatat gggtgtcaat
     3661 aaccagagca aggaaggaag gtgtccatgg aaggatcatg aggcagctcc ctggatttct
     3721 gaaaagccta aaaagagagg caatgaaggc aaaagcaaaa agtttaaaaa taattattcc
     3781 acacagcctg ctagaatgga gaggaaggaa gaaatcctta acccaccttt tgaagggaag
     3841 gatggagata ctggtagtat tccccataaa agcaaggaaa taggatttac tttccccaaa
     3901 atgcatgatt cttcgttctc acatacacca gatacaccca cagtggaagc agttgacagg
     3961 aagggtggaa attttcaggt taattttgtt gagcttggga ctcttgggga aaacaagata
     4021 agcacagtca aggcttctac tgttactgaa ccacctgcca aggtgacaga tgtgagctgc
     4081 caagagcaaa tccagggggc aggatttgtt ccttcagtag tatctgagga gaataagaca
     4141 gatgcagcca atagatacac tgcagtggct gacaaaccaa gtaaaaggag taatgatgga
     4201 aaaagtaaaa aggttaaaaa tagttctcct gagaagcaca ttctggagaa taagatagat
     4261 gcaacaaaaa tacatgttcc catggaaacc acaggggacc agggaattga aggaatggcc
     4321 tatatggacg aaaatagaaa tattacattt acctgtccca gaacaccatc agagctgata
     4381 aataaatcat ctcctctaga ggttctggaa tcagcagcct gtgaaaaact gcccactcct
     4441 actcctcaag tagtaaagga aggtgattcc tttccagata ccttggcaaa aaatgggcaa
     4501 gagatagccc cagcccagat ttccaaatca ttaatggtag ataactacac caaagatgga
     4561 gtcccaggtc aagaaagacc caagggtccc tctgctgttg tgccctctac aagcacagga
     4621 ggagttgctc tacctattac aacagccata gaaacagtta acattcatgg agatcactct
     4681 cttaagaata aagctgagct tgctgattcc atgaaaaatg aagcagggat cgatgaaggg
     4741 catgtgatag gagaatctga gtcagtgcac agtggtgcgt ctaagcattc agtagagaaa
     4801 gtcacagagc tagcaaaagg tcacctcctt cctggagtgc cagtagaaga ccagagccta
     4861 ccaggagagg ccagagccct agaaggatat gcagatagag gtaatttccc agcacatcca
     4921 gtgaatgaag agaaagagac taaagaaggg tctgttgcag ttcagattcc tgacttactg
     4981 gaagacaaag cacaaaagct cagtttttgt gaggaccaaa atgctcaaga tagaaattcc
     5041 aaaggttcag atagtttgaa taagaaggta gatctgactc ttttgtctcc aaaaagtgaa
     5101 aatgataaat tgaaagaaat tagtctggct tgtaaaatca cggaattgga aagcgtttcc
     5161 ttgccaacac cagaaatcca gtcagatttc ttacatagca aagtcgaagc tcctccttca
     5221 gaggtggcgg atacgttagt aataatgact gcttccaagg gtgttcgact cccagaaccc
     5281 aaagataaga ttttggagac acctcagaaa atgacagaaa aatctgaatc aaagacacca
     5341 ggagaaggga aaaaggaaga taaaagcaga atggcagaac caatgaaagg ctacatgaga
     5401 cccaccaagt cccgaggact tactccactt ttgccaaagt ctacaatcca ggaacaagag
     5461 agacataagc aactgaagtc cgctgtttgc ttgagctcat caactgtcta ccagcagctg
     5521 ggaatgtcag tttatggaat agccaggcca gaagaaggaa ggcctgtggt gagtgggaca
     5581 ggaaatgaca tcaccacccc accgaacaag gagctcccac caagcccaga gaagaaaaca
     5641 aagcctttgg ccaccactca acctgcaaag acttcaacat cgaaagccaa aacacagccc
     5701 acttctctcc ctaagcagcc agctcccacc accattggtg ggttgaataa aaaacccatg
     5761 agccttgctt caggcttagt gccagctgcc ccacccaaac gccctgccgt cgcctctgcc
     5821 aggccttcca tcttaccttc aaaagacgtg aagccaaagc ccattgcaga tgcaaaggct
     5881 cctgagaagc gggcctcacc atccaagcca gcttctgccc cagcctccag atctgggtcc
     5941 aagagcactc agactgttgc aaaaaccaca acagctgctg ctgttgcctc aactggccca
     6001 agcagtagga gcccctccac gctcctgccc aagaagccca ctgccattaa gactgaggga
     6061 aaacctgcag aagtcaagaa gatgactgca aagtctgtac cagctgactt gagtcgccca
     6121 aagagcacct ccaccagttc catgaagaaa accaccactc tcagtgggac agcccccgct
     6181 gcaggggtgg ttcccagccg agtcaaggcc acacccatgc cctcccggcc ctccacaact
     6241 cctttcatag acaagaagcc cacctcggcc aaacccagct ccaccacccc ccggctcagc
     6301 cgcctggcca ccaatacttc tgctcctgat ctgaagaatg tccgctccaa ggttggctcc
     6361 acggaaaaca tcaagcatca gcctggagga ggccgggcca aagtagagaa aaaaacagag
     6421 gcagctgcta caacccgaaa gcctgaatct aatgcagtca ctaaaacagc cggcccaatt
     6481 gcaagtgcac agaaacaacc tgcggggaaa gtccagatag tctccaaaaa agtgagctac
     6541 agccatattc agtccaagtg tggttccaag gacaatatta agcatgtccc tggaggtggt
     6601 aatgttcaga ttcagaacaa gaaagtggac atctctaagg tctcctccaa gtgtgggtct
     6661 aaggctaaca tcaagcacaa gcctggtgga ggagatgtca agattgaaag tcagaagttg
     6721 aacttcaagg agaaggccca ggccaaggtg ggatccctcg ataatgtggg ccacctacct
     6781 gcaggaggtg ctgtgaagac tgagggcggt ggcagcgagg ctcctctgtg tccgggtccc
     6841 cctgctgggg aggagccggc catctctgag gcagcgcctg aagctggcgc ccccacttca
     6901 gccagtggcc tcaatggcca ccccaccctg tcagggggtg gtgaccaaag ggaggcccag
     6961 accttggaca gccagatcca ggagacaaat tgagacctac aggctgacgt tccgggcaaa
     7021 tgccagggcc cgcaccgacc acggggccga cattgtctcc cgccccccac acttccctgg
     7081 cggccccaac tcgggctccc gggtccttgg ccccctttcc cgggctgtcc actagaccag
     7141 tgagcgcttg ggcgccgtgc tgggcagccc gctaggctcg ccttccctcc tgctttgcgt
     7201 gcccggggca gcagcagccc tgccccacac ctcctctcac tccccagcct gggcccatct
     7261 ccctgctttg gtcttgcccc atcactgcgc cactgctccg tggaggaggt tgggaggggg
     7321 ttggggtggt tgaggctaag ttgggatcta ggagaggaga accagattct atcctcatct
     7381 ttttttggtt ctttggtcca aacccaaaag aaactgacat gccctccctt ctccctggat
     7441 ctacctggag ggaagagtgg aggtggattc cgagtggtga caggacgctg accgtggagc
     7501 ttaagccact gcctctccct ctggtcccac aaatgggcgc ccccccctcc ccatgcaggt
     7561 ggtgtcgggc ccttcttgct gccctgcccc aagttggggg tcagtgctgc ctgtccccat
     7621 gcttaacata cccgcctagc tgctgtcaca tttttcttgt tttgtccttt tatttttttc
     7681 taataaccta aaaactggca aaatagttct gcaggttgaa gccatgtcta catgaaagtc
     7741 ctcagtaagt gttagaggga acagggcgga gatatcctta tgccaccccc gctggaggat
     7801 gtgggcagct tagggccctg gaggcggtgc ggcagggaag aggggtgcag aggctgtggc
     7861 tggtgagccg gtcaggcaca caaggggccc ttggagcgtg gactggttgg ttttgccatt
     7921 ttgttgtgtg tatgctgctt ttcttttcta accaagaggc tggttttggc atctctgtcc
     7981 cattccctgg gatctggtgg tcagccctag gataaaaagc cagggctgga gaacaagaaa
     8041 gggccaggag atggaattcc ttcaggccgg cacccacacc ctaggacatg taagccctca
     8101 tgtccaaggg agcctcatgc agatagtagg aaatcaggtc tggaaattta aaaataaaag
     8161 gcatgagact aaggctatct gcttccctta tgccctgact ggagagggga gggaggagag
     8221 gcaaggccca cagagggcat cccagctagg ccttgggatg gctgcagtga ggagaaatcc
     8281 cgggaactgt attgacacaa agattcttat tgcacttgta ttttttgtat taaagtttgc
     8341 atggtttcta ataaaggatt caaacataag tttgtagtga aatggcctgg gagattccaa
     8401 gggcttctct ggagggggat tggctgcagt gtagatttgc ctctgaggag gctgccccag
     8461 acttggcctc ctcatgcccc ctcctgacct ctgcccttct ctggtcctgg catccctgga
     8521 gaaggtaggg gtcttgacct aagtttagat ttgatctcca tgtgcaggga ggctgtcctg
     8581 ggcctgacag gtcctccccc tttctgaggt agcagtgcct tgtggaggtt tgacaccatg
     8641 tccctagctc cccaagcaca caccaggaaa ctgcaggggc tcacggagga agtgctgcct
     8701 gggccagggg gaccagcttt cctccgtaga gaccatgtgc agaacacttc tgctgtgcca
     8761 agaacatgag ggagccagtg ttttgtcagc aggaagaaag ggcctgctgg gatgaaagtg
     8821 ggaaggaaac agggttgcgt agtcaggaga cacctcaggg gcaacagcac aggcccagag
     8881 tacctgctgc ctccactgcg tctgtcctgg ggtcatgagg atgctgaggt tgacgacagg
     8941 ttccaggtcc tttcactcct ttggccaaag gttgggggta ggtggcccaa gtggcgtgct
     9001 ctctaggtag acaacaggag tggtcagagt tccctcaaag gatcctccac tccagagcac
     9061 ctgagaaggc cgggaccaga ggccctgtgt gatgtgtact ccgcagctgt ttggggtggg
     9121 acatttctgt acttctcgat ttgcttatgg ctcagccatt acctgtgtca gtccatgatt
     9181 ctgttgtaac agttttaaga gtaaataaat aaagctgcct gatgtcccat ca
//
