LOCUS       NM_001384782            5777 bp    mRNA    linear   PRI 21-SEP-2024
DEFINITION  Homo sapiens microtubule associated protein 4 (MAP4), transcript
            variant 43, mRNA.
ACCESSION   NM_001384782
VERSION     NM_001384782.1
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5777)
  AUTHORS   Thapa,N., Chen,M., Cryns,V.L. and Anderson,R.
  TITLE     A p85 isoform switch enhances PI3K activation on endosomes by a
            MAP4- and PI3P-dependent mechanism
  JOURNAL   Cell Rep 43 (5), 114119 (2024)
   PUBMED   38630589
  REMARK    GeneRIF: A p85 isoform switch enhances PI3K activation on endosomes
            by a MAP4- and PI3P-dependent mechanism.
REFERENCE   2  (bases 1 to 5777)
  AUTHORS   Xia,X., Ge,Y., Ge,F., Gu,P., Liu,Y., Li,P. and Xu,P.
  TITLE     MAP4 acts as an oncogene and prognostic marker and affects
            radioresistance by mediating epithelial-mesenchymal transition in
            lung adenocarcinoma
  JOURNAL   J Cancer Res Clin Oncol 150 (2), 88 (2024)
   PUBMED   38341398
  REMARK    GeneRIF: MAP4 acts as an oncogene and prognostic marker and affects
            radioresistance by mediating epithelial-mesenchymal transition in
            lung adenocarcinoma.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 5777)
  AUTHORS   Nishida,K., Matsumura,K., Tamura,M., Nakamichi,T., Shimamori,K.,
            Kuragano,M., Kabir,A.M.R., Kakugo,A., Kotani,S., Nishishita,N. and
            Tokuraku,K.
  TITLE     Effects of three microtubule-associated proteins (MAP2, MAP4, and
            Tau) on microtubules' physical properties and neurite morphology
  JOURNAL   Sci Rep 13 (1), 8870 (2023)
   PUBMED   37258650
  REMARK    GeneRIF: Effects of three microtubule-associated proteins (MAP2,
            MAP4, and Tau) on microtubules' physical properties and neurite
            morphology.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 5777)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   5  (bases 1 to 5777)
  AUTHORS   Wang,L., Paudyal,S.C., Kang,Y., Owa,M., Liang,F.X., Spektor,A.,
            Knaut,H., Sanchez,I. and Dynlacht,B.D.
  TITLE     Regulators of tubulin polyglutamylation control nuclear shape and
            cilium disassembly by balancing microtubule and actin assembly
  JOURNAL   Cell Res 32 (2), 190-209 (2022)
   PUBMED   34782749
REFERENCE   6  (bases 1 to 5777)
  AUTHORS   Ookata,K., Hisanaga,S., Bulinski,J.C., Murofushi,H., Aizawa,H.,
            Itoh,T.J., Hotani,H., Okumura,E., Tachibana,K. and Kishimoto,T.
  TITLE     Cyclin B interaction with microtubule-associated protein 4 (MAP4)
            targets p34cdc2 kinase to microtubules and is a potential regulator
            of M-phase microtubule dynamics
  JOURNAL   J Cell Biol 128 (5), 849-862 (1995)
   PUBMED   7876309
REFERENCE   7  (bases 1 to 5777)
  AUTHORS   Chapin,S.J., Lue,C.M., Yu,M.T. and Bulinski,J.C.
  TITLE     Differential expression of alternatively spliced forms of MAP4: a
            repertoire of structurally different microtubule-binding domains
  JOURNAL   Biochemistry 34 (7), 2289-2301 (1995)
   PUBMED   7857940
REFERENCE   8  (bases 1 to 5777)
  AUTHORS   Chapin,S.J. and Bulinski,J.C.
  TITLE     Microtubule stabilization by assembly-promoting
            microtubule-associated proteins: a repeat performance
  JOURNAL   Cell Motil Cytoskeleton 23 (4), 236-243 (1992)
   PUBMED   1477887
  REMARK    Review article
REFERENCE   9  (bases 1 to 5777)
  AUTHORS   West,R.R., Tenbarge,K.M. and Olmsted,J.B.
  TITLE     A model for microtubule-associated protein 4 structure. Domains
            defined by comparisons of human, mouse, and bovine sequences
  JOURNAL   J Biol Chem 266 (32), 21886-21896 (1991)
   PUBMED   1718985
REFERENCE   10 (bases 1 to 5777)
  AUTHORS   Chapin,S.J. and Bulinski,J.C.
  TITLE     Non-neuronal 210 x 10(3) Mr microtubule-associated protein (MAP4)
            contains a domain homologous to the microtubule-binding domains of
            neuronal MAP2 and tau
  JOURNAL   J Cell Sci 98 (Pt 1), 27-36 (1991)
   PUBMED   1905296
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC124916.3 and AC139667.5.
            
            Summary: The protein encoded by this gene is a major non-neuronal
            microtubule-associated protein. This protein contains a domain
            similar to the microtubule-binding domains of neuronal
            microtubule-associated protein (MAP2) and microtubule-associated
            protein tau (MAPT/TAU). This protein promotes microtubule assembly,
            and has been shown to counteract destabilization of interphase
            microtubule catastrophe promotion. Cyclin B was found to interact
            with this protein, which targets cell division cycle 2 (CDC2)
            kinase to microtubules. The phosphorylation of this protein affects
            microtubule properties and cell cycle progression. Multiple
            transcript variants encoding different isoforms have been found for
            this gene. [provided by RefSeq, Aug 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR14038195.496949.1,
                                           SRR14038196.1802205.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-76                AC124916.3         113569-113644       c
            77-318              AC124916.3         23434-23675         c
            319-441             AC139667.5         48903-49025
            442-555             AC139667.5         55375-55488
            556-678             AC139667.5         58412-58534
            679-1902            AC139667.5         60079-61302
            1903-2025           AC139667.5         62314-62436
            2026-2132           AC139667.5         99730-99836
            2133-2348           AC139667.5         101353-101568
            2349-2532           AC139667.5         105153-105336
            2533-2592           AC139667.5         105967-106026
            2593-2840           AC139667.5         106148-106395
            2841-2954           AC139667.5         107926-108039
            2955-3047           AC139667.5         109915-110007
            3048-3129           AC139667.5         119741-119822
            3130-3242           AC139667.5         121893-122005
            3243-3432           AC139667.5         123901-124090
            3433-5777           AC139667.5         124214-126558
FEATURES             Location/Qualifiers
     source          1..5777
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="3"
                     /map="3p21.31"
     gene            1..5777
                     /gene="MAP4"
                     /note="microtubule associated protein 4"
                     /db_xref="GeneID:4134"
                     /db_xref="HGNC:HGNC:6862"
                     /db_xref="MIM:157132"
     exon            1..76
                     /gene="MAP4"
                     /inference="alignment:Splign:2.1.0"
     exon            77..318
                     /gene="MAP4"
                     /inference="alignment:Splign:2.1.0"
     CDS             96..3440
                     /gene="MAP4"
                     /note="isoform 41 is encoded by transcript variant 43;
                     MAP-4"
                     /codon_start=1
                     /product="microtubule-associated protein 4 isoform 41"
                     /protein_id="NP_001371711.1"
                     /db_xref="GeneID:4134"
                     /db_xref="HGNC:HGNC:6862"
                     /db_xref="MIM:157132"
                     /translation="MADLSLADALTEPSPDIEGEIKRDFIATLEAEAFDDVVGETVGK
                     TDYIPLLDVDEKTGNSESKKKPCSETSQIEGSPTEFLEEKMAYQEYPNSQNWPEDTNF
                     CFQPEQVVDPIQTDPFKMYHDDDLADLVFPSSATADTSIFAGQNDPLKDSYGMSPCNT
                     AVVPQGWSVEALNSPHSESFVSPEAVAEPPQPTAVPLELAKEIEMASEERPPAQALEI
                     MMGLKTTDMAPSKETEMALAKDMALATKTEVALAKDMESPTKLDVTLAKDMQPSMESD
                     MALVKDMELPTEKEVALVKDVRWPTETDVSSAKNVVLPTETEVAPAKDVTLLKETERA
                     SPIKMDLAPSKDMGPPKENKKETERASPIKMDLAPSKDMGPPKENKIVPAKDLVLLSE
                     IEVAQANDIISSTEISSAEKVALSSETEVALARDMTLPPETNVILTKDKALPLEAEVA
                     PVKDMAQLPETEIAPAKDVAPSTVKEVGLLKDMSPLSETEMALGKDVTPPPETEVVLI
                     KNVCLPPEMEVALTEDQVPALKTEAPLAKDGVLTLANNVTPAKDVPPLSETEATPVPI
                     KDMEIAQTQKGISEDSHLESLQDVGQSAAPTFMISPETVTGTGKKCSLPAEEDSVLEK
                     LGERKPCNSQPSELSSETSGIARPEEGRPVVSGTGNDITTPPNKELPPSPEKKTKPLA
                     TTQPAKTSTSKAKTQPTSLPKQPAPTTIGGLNKKPMSLASGLVPAAPPKRPAVASARP
                     SILPSKDVKPKPIADAKAPEKRASPSKPASAPASRSGSKSTQTVAKTTTAAAVASTGP
                     SSRSPSTLLPKKPTAIKTEGKPAEVKKMTAKSVPADLSRPKSTSTSSMKKTTTLSGTA
                     PAAGVVPSRVKATPMPSRPSTTPFIDKKPTSAKPSSTTPRLSRLATNTSAPDLKNVRS
                     KAKVEKKTEAAATTRKPESNAVTKTAGPIASAQKQPAGKVQIVSKKVSYSHIQSKCGS
                     KDNIKHVPGGGNVQIQNKKVDISKVSSKCGSKANIKHKPGGGDVKIESQKLNFKEKAQ
                     AKVGSLDNVGHLPAGGAVKTEGGGSEAPLCPGPPAGEEPAISEAAPEAGAPTSASGLN
                     GHPTLSGGGDQREAQTLDSQIQETSI"
     exon            319..441
                     /gene="MAP4"
                     /inference="alignment:Splign:2.1.0"
     exon            442..555
                     /gene="MAP4"
                     /inference="alignment:Splign:2.1.0"
     exon            556..678
                     /gene="MAP4"
                     /inference="alignment:Splign:2.1.0"
     exon            679..1902
                     /gene="MAP4"
                     /inference="alignment:Splign:2.1.0"
     exon            1903..2025
                     /gene="MAP4"
                     /inference="alignment:Splign:2.1.0"
     exon            2026..2132
                     /gene="MAP4"
                     /inference="alignment:Splign:2.1.0"
     exon            2133..2348
                     /gene="MAP4"
                     /inference="alignment:Splign:2.1.0"
     exon            2349..2532
                     /gene="MAP4"
                     /inference="alignment:Splign:2.1.0"
     exon            2533..2592
                     /gene="MAP4"
                     /inference="alignment:Splign:2.1.0"
     exon            2593..2840
                     /gene="MAP4"
                     /inference="alignment:Splign:2.1.0"
     exon            2841..2954
                     /gene="MAP4"
                     /inference="alignment:Splign:2.1.0"
     exon            2955..3047
                     /gene="MAP4"
                     /inference="alignment:Splign:2.1.0"
     exon            3048..3129
                     /gene="MAP4"
                     /inference="alignment:Splign:2.1.0"
     exon            3130..3242
                     /gene="MAP4"
                     /inference="alignment:Splign:2.1.0"
     exon            3243..3432
                     /gene="MAP4"
                     /inference="alignment:Splign:2.1.0"
     exon            3433..5777
                     /gene="MAP4"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4895..4900
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MAP4"
                     /note="hexamer: AATAAA"
     polyA_site      4914
                     /gene="MAP4"
                     /note="major polyA site"
     regulatory      5753..5758
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MAP4"
                     /note="hexamer: AATAAA"
     polyA_site      5777
                     /gene="MAP4"
                     /note="major polyA site"
ORIGIN      
        1 gtctcctacc tcctcacggc tcttcccggc gctctcctgg ctcccttctg ccccagctcc
       61 gtctcggcgg cggcgggcag ttgcagtggt gcagaatggc tgacctcagt cttgcagatg
      121 cattaacaga accatctcca gacattgagg gagagataaa gcgggacttc attgccacac
      181 tagaggcaga ggcctttgat gatgttgtgg gagaaactgt tggaaaaaca gactatattc
      241 ctctcctgga tgttgatgag aaaaccggga actcagagtc aaagaagaaa ccgtgctcag
      301 aaactagcca gattgaaggg tctccaactg aattccttga agagaaaatg gcctaccagg
      361 aatacccaaa tagccagaac tggccagaag ataccaactt ttgtttccaa cctgagcaag
      421 tggtcgatcc tatccagact gatcccttta agatgtacca tgatgatgac ctggcagatt
      481 tggtctttcc ctccagtgcg acagctgata cttcaatatt tgcaggacaa aatgatccct
      541 tgaaagacag ttacggtatg tctccctgca acacagctgt tgtacctcag gggtggtctg
      601 tggaagcctt aaactctcca cactcagagt cctttgtttc cccagaggct gttgcagaac
      661 ctcctcagcc aacggcagtt cccttagagc tagccaagga gatagaaatg gcatcagaag
      721 agaggccacc agcacaagca ttggaaataa tgatgggact gaagactact gacatggcac
      781 catctaaaga aacagagatg gccctcgcca aggacatggc actagctaca aaaaccgagg
      841 tggcattggc taaagatatg gaatcaccca ccaaattaga tgtgacactg gccaaggaca
      901 tgcagccatc catggaatca gatatggccc tagtcaagga catggaacta cccacagaaa
      961 aagaagtggc cctggttaag gatgtcagat ggcccacaga aacagatgta tcttcagcca
     1021 agaatgtggt actgcccaca gaaacagagg tagccccagc caaggatgtg acactgttga
     1081 aagaaacaga gagggcatct cctataaaaa tggacttagc cccttccaag gacatgggac
     1141 cacccaaaga aaacaagaaa gaaacagaga gggcatctcc tataaaaatg gacttggctc
     1201 cttccaagga catgggacca cccaaagaaa acaagatagt cccagccaag gatttggtat
     1261 tactctcaga aatagaggtg gcacaggcta atgacattat atcatccaca gaaatatcct
     1321 ctgctgagaa ggtggctttg tcctcagaaa cagaggtagc cctggccagg gacatgacac
     1381 tgcccccgga aaccaacgtg atcttgacca aggataaagc actaccttta gaagcagagg
     1441 tggccccagt caaggacatg gctcaactcc cagaaacaga aatagccccg gccaaggatg
     1501 tggctccgtc cacagtaaaa gaagtgggct tgttgaagga catgtctcca ctatcagaaa
     1561 cagaaatggc tctgggcaag gatgtgactc cacctccaga aacagaagta gttctcatca
     1621 agaacgtatg tctgcctcca gaaatggagg tggccctgac tgaggatcag gtcccagccc
     1681 tcaaaacaga agcacccctg gctaaggatg gggttctgac cctggccaac aatgtgactc
     1741 cagccaaaga tgttccacca ctctcagaaa cagaggcaac accagttcca attaaagaca
     1801 tggaaattgc acaaacacaa aaaggaataa gtgaggattc ccatttagaa tctctgcagg
     1861 atgtggggca gtcagctgca cctactttca tgatttcacc agaaaccgtc acaggaacgg
     1921 ggaaaaagtg cagcttgccg gccgaggagg attctgtgtt agaaaaacta ggggaaagga
     1981 aaccatgcaa cagtcaacct tctgagcttt cttcagagac ctcaggaata gccaggccag
     2041 aagaaggaag gcctgtggtg agtgggacag gaaatgacat caccacccca ccgaacaagg
     2101 agctcccacc aagcccagag aagaaaacaa agcctttggc caccactcaa cctgcaaaga
     2161 cttcaacatc gaaagccaaa acacagccca cttctctccc taagcagcca gctcccacca
     2221 ccattggtgg gttgaataaa aaacccatga gccttgcttc aggcttagtg ccagctgccc
     2281 cacccaaacg ccctgccgtc gcctctgcca ggccttccat cttaccttca aaagacgtga
     2341 agccaaagcc cattgcagat gcaaaggctc ctgagaagcg ggcctcacca tccaagccag
     2401 cttctgcccc agcctccaga tctgggtcca agagcactca gactgttgca aaaaccacaa
     2461 cagctgctgc tgttgcctca actggcccaa gcagtaggag cccctccacg ctcctgccca
     2521 agaagcccac tgccattaag actgagggaa aacctgcaga agtcaagaag atgactgcaa
     2581 agtctgtacc agctgacttg agtcgcccaa agagcacctc caccagttcc atgaagaaaa
     2641 ccaccactct cagtgggaca gcccccgctg caggggtggt tcccagccga gtcaaggcca
     2701 cacccatgcc ctcccggccc tccacaactc ctttcataga caagaagccc acctcggcca
     2761 aacccagctc caccaccccc cggctcagcc gcctggccac caatacttct gctcctgatc
     2821 tgaagaatgt ccgctccaag gccaaagtag agaaaaaaac agaggcagct gctacaaccc
     2881 gaaagcctga atctaatgca gtcactaaaa cagccggccc aattgcaagt gcacagaaac
     2941 aacctgcggg gaaagtccag atagtctcca aaaaagtgag ctacagccat attcagtcca
     3001 agtgtggttc caaggacaat attaagcatg tccctggagg tggtaatgtt cagattcaga
     3061 acaagaaagt ggacatctct aaggtctcct ccaagtgtgg gtctaaggct aacatcaagc
     3121 acaagcctgg tggaggagat gtcaagattg aaagtcagaa gttgaacttc aaggagaagg
     3181 cccaggccaa ggtgggatcc ctcgataatg tgggccacct acctgcagga ggtgctgtga
     3241 agactgaggg cggtggcagc gaggctcctc tgtgtccggg tccccctgct ggggaggagc
     3301 cggccatctc tgaggcagcg cctgaagctg gcgcccccac ttcagccagt ggcctcaatg
     3361 gccaccccac cctgtcaggg ggtggtgacc aaagggaggc ccagaccttg gacagccaga
     3421 tccaggagac aagcatctaa tgatgacatt ctggtctcgt cttccgtctc ccccgtgttc
     3481 ccctcttgtc tcccctgttc ccctctccct tccctcctcc catgtcactg cagattgaga
     3541 cctacaggct gacgttccgg gcaaatgcca gggcccgcac cgaccacggg gccgacattg
     3601 tctcccgccc cccacacttc cctggcggcc ccaactcggg ctcccgggtc cttggccccc
     3661 tttcccgggc tgtccactag accagtgagc gcttgggcgc cgtgctgggc agcccgctag
     3721 gctcgccttc cctcctgctt tgcgtgcccg gggcagcagc agccctgccc cacacctcct
     3781 ctcactcccc agcctgggcc catctccctg ctttggtctt gccccatcac tgcgccactg
     3841 ctccgtggag gaggttggga gggggttggg gtggttgagg ctaagttggg atctaggaga
     3901 ggagaaccag attctatcct catctttttt tggttctttg gtccaaaccc aaaagaaact
     3961 gacatgccct cccttctccc tggatctacc tggagggaag agtggaggtg gattccgagt
     4021 ggtgacagga cgctgaccgt ggagcttaag ccactgcctc tccctctggt cccacaaatg
     4081 ggcgcccccc cctccccatg caggtggtgt cgggcccttc ttgctgccct gccccaagtt
     4141 gggggtcagt gctgcctgtc cccatgctta acatacccgc ctagctgctg tcacattttt
     4201 cttgttttgt ccttttattt ttttctaata acctaaaaac tggcaaaata gttctgcagg
     4261 ttgaagccat gtctacatga aagtcctcag taagtgttag agggaacagg gcggagatat
     4321 ccttatgcca cccccgctgg aggatgtggg cagcttaggg ccctggaggc ggtgcggcag
     4381 ggaagagggg tgcagaggct gtggctggtg agccggtcag gcacacaagg ggcccttgga
     4441 gcgtggactg gttggttttg ccattttgtt gtgtgtatgc tgcttttctt ttctaaccaa
     4501 gaggctggtt ttggcatctc tgtcccattc cctgggatct ggtggtcagc cctaggataa
     4561 aaagccaggg ctggagaaca agaaagggcc aggagatgga attccttcag gccggcaccc
     4621 acaccctagg acatgtaagc cctcatgtcc aagggagcct catgcagata gtaggaaatc
     4681 aggtctggaa atttaaaaat aaaaggcatg agactaaggc tatctgcttc ccttatgccc
     4741 tgactggaga ggggagggag gagaggcaag gcccacagag ggcatcccag ctaggccttg
     4801 ggatggctgc agtgaggaga aatcccggga actgtattga cacaaagatt cttattgcac
     4861 ttgtattttt tgtattaaag tttgcatggt ttctaataaa ggattcaaac ataagtttgt
     4921 agtgaaatgg cctgggagat tccaagggct tctctggagg gggattggct gcagtgtaga
     4981 tttgcctctg aggaggctgc cccagacttg gcctcctcat gccccctcct gacctctgcc
     5041 cttctctggt cctggcatcc ctggagaagg taggggtctt gacctaagtt tagatttgat
     5101 ctccatgtgc agggaggctg tcctgggcct gacaggtcct ccccctttct gaggtagcag
     5161 tgccttgtgg aggtttgaca ccatgtccct agctccccaa gcacacacca ggaaactgca
     5221 ggggctcacg gaggaagtgc tgcctgggcc agggggacca gctttcctcc gtagagacca
     5281 tgtgcagaac acttctgctg tgccaagaac atgagggagc cagtgttttg tcagcaggaa
     5341 gaaagggcct gctgggatga aagtgggaag gaaacagggt tgcgtagtca ggagacacct
     5401 caggggcaac agcacaggcc cagagtacct gctgcctcca ctgcgtctgt cctggggtca
     5461 tgaggatgct gaggttgacg acaggttcca ggtcctttca ctcctttggc caaaggttgg
     5521 gggtaggtgg cccaagtggc gtgctctcta ggtagacaac aggagtggtc agagttccct
     5581 caaaggatcc tccactccag agcacctgag aaggccggga ccagaggccc tgtgtgatgt
     5641 gtactccgca gctgtttggg gtgggacatt tctgtacttc tcgatttgct tatggctcag
     5701 ccattacctg tgtcagtcca tgattctgtt gtaacagttt taagagtaaa taaataaagc
     5761 tgcctgatgt cccatca
//
