LOCUS       NM_001284230           11511 bp    mRNA    linear   PRI 07-APR-2024
DEFINITION  Homo sapiens mitogen-activated protein kinase kinase kinase 9
            (MAP3K9), transcript variant 2, mRNA.
ACCESSION   NM_001284230 XM_005267682
VERSION     NM_001284230.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 11511)
  AUTHORS   Segal,D., Maier,S., Mastromarco,G.J., Qian,W.W., Nabeel-Shah,S.,
            Lee,H., Moore,G., Lacoste,J., Larsen,B., Lin,Z.Y.,
            Selvabaskaran,A., Liu,K., Smibert,C., Zhang,Z., Greenblatt,J.,
            Peng,J., Lee,H.O., Gingras,A.C. and Taipale,M.
  TITLE     A central chaperone-like role for 14-3-3 proteins in human cells
  JOURNAL   Mol Cell 83 (6), 974-993 (2023)
   PUBMED   36931259
REFERENCE   2  (bases 1 to 11511)
  AUTHORS   Liang,L., Xu,W.Y., Shen,A., Cen,H.Y., Chen,Z.J., Tan,L.,
            Zhang,L.M., Zhang,Y., Fu,J.J., Qin,A.P., Lei,X.P., Li,S.P.,
            Qin,Y.Y., Huang,J.H. and Yu,X.Y.
  TITLE     Promoter methylation-regulated miR-148a-3p inhibits lung
            adenocarcinoma (LUAD) progression by targeting MAP3K9
  JOURNAL   Acta Pharmacol Sin 43 (11), 2946-2955 (2022)
   PUBMED   35388129
  REMARK    GeneRIF: Promoter methylation-regulated miR-148a-3p inhibits lung
            adenocarcinoma (LUAD) progression by targeting MAP3K9.
REFERENCE   3  (bases 1 to 11511)
  AUTHORS   Zhou,J.L., Deng,S., Fang,H.S., Peng,H. and Hu,Q.J.
  TITLE     CircSPI1_005 ameliorates osteoarthritis by sponging miR-370-3p to
            regulate the expression of MAP3K9
  JOURNAL   Int Immunopharmacol 110, 109064 (2022)
   PUBMED   35978511
  REMARK    GeneRIF: CircSPI1_005 ameliorates osteoarthritis by sponging
            miR-370-3p to regulate the expression of MAP3K9.
REFERENCE   4  (bases 1 to 11511)
  AUTHORS   Chen,F., Lao,Z., Zhang,H.Y., Wang,J. and Wang,S.Z.
  TITLE     Elevation of miR-125b-5p is related to improved prognosis in
            laryngeal squamous cell carcinoma and inhibits the malignancy and
            glycometabolic disorder by targeting MAP3K9
  JOURNAL   Neoplasma 69 (3), 550-559 (2022)
   PUBMED   35188403
  REMARK    GeneRIF: Elevation of miR-125b-5p is related to improved prognosis
            in laryngeal squamous cell carcinoma and inhibits the malignancy
            and glycometabolic disorder by targeting MAP3K9.
REFERENCE   5  (bases 1 to 11511)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   6  (bases 1 to 11511)
  AUTHORS   Figueroa,C., Tarras,S., Taylor,J. and Vojtek,A.B.
  TITLE     Akt2 negatively regulates assembly of the POSH-MLK-JNK signaling
            complex
  JOURNAL   J Biol Chem 278 (48), 47922-47927 (2003)
   PUBMED   14504284
REFERENCE   7  (bases 1 to 11511)
  AUTHORS   Xu,Z., Maroney,A.C., Dobrzanski,P., Kukekov,N.V. and Greene,L.A.
  TITLE     The MLK family mediates c-Jun N-terminal kinase activation in
            neuronal apoptosis
  JOURNAL   Mol Cell Biol 21 (14), 4713-4724 (2001)
   PUBMED   11416147
REFERENCE   8  (bases 1 to 11511)
  AUTHORS   Dorow,D.S., Devereux,L., Tu,G.F., Price,G., Nicholl,J.K.,
            Sutherland,G.R. and Simpson,R.J.
  TITLE     Complete nucleotide sequence, expression, and chromosomal
            localisation of human mixed-lineage kinase 2
  JOURNAL   Eur J Biochem 234 (2), 492-500 (1995)
   PUBMED   8536694
REFERENCE   9  (bases 1 to 11511)
  AUTHORS   Gallo,K.A., Mark,M.R., Scadden,D.T., Wang,Z., Gu,Q. and
            Godowski,P.J.
  TITLE     Identification and characterization of SPRK, a novel src-homology 3
            domain-containing proline-rich kinase with serine/threonine kinase
            activity
  JOURNAL   J Biol Chem 269 (21), 15092-15100 (1994)
   PUBMED   8195146
REFERENCE   10 (bases 1 to 11511)
  AUTHORS   Dorow,D.S., Devereux,L., Dietzsch,E. and De Kretser,T.
  TITLE     Identification of a new family of human epithelial protein kinases
            containing two leucine/isoleucine-zipper domains
  JOURNAL   Eur J Biochem 213 (2), 701-710 (1993)
   PUBMED   8477742
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC004816.1.
            
            On Aug 5, 2020 this sequence version replaced NM_001284230.1.
            
            Transcript Variant: This variant (2) lacks an alternate in-frame
            exon in the 3' coding region, compared to variant 1. The encoded
            isoform (2) is shorter than isoform 1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AY327900.1, BC111407.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000554752.7/ ENSP00000451612.2
            RefSeq Select criteria :: based on manual assertion, conservation
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-748               AC004816.1         17860-18607
            749-1162            AC004816.1         26293-26706
            1163-1343           AC004816.1         66191-66371
            1344-1492           AC004816.1         77292-77440
            1493-1668           AC004816.1         78369-78544
            1669-1909           AC004816.1         84782-85022
            1910-2032           AC004816.1         87209-87331
            2033-2186           AC004816.1         88975-89128
            2187-2255           AC004816.1         91344-91412
            2256-2368           AC004816.1         92875-92987
            2369-3172           AC004816.1         94031-94834
            3173-11511          AC004816.1         96509-104847
FEATURES             Location/Qualifiers
     source          1..11511
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="14"
                     /map="14q24.2"
     gene            1..11511
                     /gene="MAP3K9"
                     /gene_synonym="MEKK9; MLK1; PRKE1"
                     /note="mitogen-activated protein kinase kinase kinase 9"
                     /db_xref="GeneID:4293"
                     /db_xref="HGNC:HGNC:6861"
                     /db_xref="MIM:600136"
     exon            1..748
                     /gene="MAP3K9"
                     /gene_synonym="MEKK9; MLK1; PRKE1"
                     /inference="alignment:Splign:2.1.0"
     CDS             343..3657
                     /gene="MAP3K9"
                     /gene_synonym="MEKK9; MLK1; PRKE1"
                     /EC_number="2.7.11.25"
                     /note="isoform 2 is encoded by transcript variant 2; mixed
                     lineage kinase 1 (tyr and ser/thr specificity)"
                     /codon_start=1
                     /product="mitogen-activated protein kinase kinase kinase 9
                     isoform 2"
                     /protein_id="NP_001271159.1"
                     /db_xref="CCDS:CCDS61488.1"
                     /db_xref="GeneID:4293"
                     /db_xref="HGNC:HGNC:6861"
                     /db_xref="MIM:600136"
                     /translation="MEPSRALLGCLASAAAAAPPGEDGAGAGAEEEEEEEEEAAAAVG
                     PGELGCDAPLPYWTAVFEYEAAGEDELTLRLGDVVEVLSKDSQVSGDEGWWTGQLNQR
                     VGIFPSNYVTPRSAFSSRCQPGGEDPSCYPPIQLLEIDFAELTLEEIIGIGGFGKVYR
                     AFWIGDEVAVKAARHDPDEDISQTIENVRQEAKLFAMLKHPNIIALRGVCLKEPNLCL
                     VMEFARGGPLNRVLSGKRIPPDILVNWAVQIARGMNYLHDEAIVPIIHRDLKSSNILI
                     LQKVENGDLSNKILKITDFGLAREWHRTTKMSAAGTYAWMAPEVIRASMFSKGSDVWS
                     YGVLLWELLTGEVPFRGIDGLAVAYGVAMNKLALPIPSTCPEPFAKLMEDCWNPDPHS
                     RPSFTNILDQLTTIEESGFFEMPKDSFHCLQDNWKHEIQEMFDQLRAKEKELRTWEEE
                     LTRAALQQKNQEELLRRREQELAEREIDILERELNIIIHQLCQEKPRVKKRKGKFRKS
                     RLKLKDGNRISLPSDFQHKFTVQASPTMDKRKSLINSRSSPPASPTIIPRLRAIQLTP
                     GESSKTWGRSSVVPKEEGEEEEKRAPKKKGRTWGPGTLGQKELASGDEGSPQRREKAN
                     GLSTPSESPHFHLGLKSLVDGYKQWSSSAPNLVKGPRSSPALPGFTSLMEMEDEDSEG
                     PGSGESRLQHSPSQSYLCIPFPRGEDGDGPSSDGIHEEPTPVNSATSTPQLTPTNSLK
                     RGGAHHRRCEVALLGCGAVLAATGLGFDLLEAGKCQLLPLEEPEPPAREEKKRREGLF
                     QRSSRPRRSTSPPSRKLFKKEEPMLLLGDPSASLTLLSLSSISECNSTRSLLRSDSDE
                     IVVYEMPVSPVEAPPLSPCTHNPLVNVRVERFKRDPNQSLTPTHVTLTTPSQPSSHRR
                     TPSDGALKPETLLASRSPSSNGLSPSPGAGMLKTPSPSRDPGEFPRLPDPNVVFPPTP
                     RRWNTQQDSTLERPKTLEFLPRPRPSANRQRLDPWWFVSPSHARSTSPANSSSTETPS
                     NLDSCFASSSSTVEERPGLPALLPFQAGPLPPTERTLLDLDAEGQSQDSTVPLCRAEL
                     NTHRPAPYEIQQEFWS"
     exon            749..1162
                     /gene="MAP3K9"
                     /gene_synonym="MEKK9; MLK1; PRKE1"
                     /inference="alignment:Splign:2.1.0"
     exon            1163..1343
                     /gene="MAP3K9"
                     /gene_synonym="MEKK9; MLK1; PRKE1"
                     /inference="alignment:Splign:2.1.0"
     exon            1344..1492
                     /gene="MAP3K9"
                     /gene_synonym="MEKK9; MLK1; PRKE1"
                     /inference="alignment:Splign:2.1.0"
     exon            1493..1668
                     /gene="MAP3K9"
                     /gene_synonym="MEKK9; MLK1; PRKE1"
                     /inference="alignment:Splign:2.1.0"
     exon            1669..1909
                     /gene="MAP3K9"
                     /gene_synonym="MEKK9; MLK1; PRKE1"
                     /inference="alignment:Splign:2.1.0"
     exon            1910..2032
                     /gene="MAP3K9"
                     /gene_synonym="MEKK9; MLK1; PRKE1"
                     /inference="alignment:Splign:2.1.0"
     exon            2033..2186
                     /gene="MAP3K9"
                     /gene_synonym="MEKK9; MLK1; PRKE1"
                     /inference="alignment:Splign:2.1.0"
     exon            2187..2255
                     /gene="MAP3K9"
                     /gene_synonym="MEKK9; MLK1; PRKE1"
                     /inference="alignment:Splign:2.1.0"
     exon            2256..2368
                     /gene="MAP3K9"
                     /gene_synonym="MEKK9; MLK1; PRKE1"
                     /inference="alignment:Splign:2.1.0"
     exon            2369..3172
                     /gene="MAP3K9"
                     /gene_synonym="MEKK9; MLK1; PRKE1"
                     /inference="alignment:Splign:2.1.0"
     exon            3173..11511
                     /gene="MAP3K9"
                     /gene_synonym="MEKK9; MLK1; PRKE1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      11490..11495
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MAP3K9"
                     /gene_synonym="MEKK9; MLK1; PRKE1"
                     /note="hexamer: AATAAA"
     polyA_site      11511
                     /gene="MAP3K9"
                     /gene_synonym="MEKK9; MLK1; PRKE1"
                     /note="major polyA site"
ORIGIN      
        1 gcggagagga gagcggcggc cggctggcag cggcgggcac cggctgcgcg agcaggtacc
       61 ggcggcagag gtgacgaggc cgcccccggg ggggcgagcg ggcaggcggg gactcggccc
      121 gctgcgcccc taggctgcgc gaagagctcg cgctcggcgc tcttctcgta ccgcggtaac
      181 agcgccggcg agccggctct cccagccctg ccagcccggg ccctacctgc ggccgccgtc
      241 gttggggggg ggtcctccca gctctgcgga ggcgcatgaa caataggcgg cggcggctcc
      301 tgcgggcggc ggcggccccg caccctatgg atcggccgct ccatggagcc ctccagagcg
      361 cttctcggct gcctagcgag cgccgccgct gccgccccgc cgggggagga tggagcaggg
      421 gccggggccg aggaggagga ggaggaggag gaggaggcgg cggcggcggt gggccccggg
      481 gagctgggct gcgacgcgcc gctgccctac tggacggccg tgttcgagta cgaggcggcg
      541 ggcgaggacg agctgaccct gcggctgggc gacgtggtgg aggtgctgtc caaggactcg
      601 caggtgtccg gcgacgaggg ctggtggacc gggcagctga accagcgggt gggcatcttc
      661 cccagcaact acgtgacccc gcgcagcgcc ttctccagcc gctgccagcc cggcggcgag
      721 gaccccagtt gctacccgcc cattcagttg ttagaaattg attttgcgga gctcaccttg
      781 gaagagatta ttggcatcgg gggctttggg aaggtctatc gtgctttctg gataggggat
      841 gaggttgctg tgaaagcagc tcgccacgac cctgatgagg acatcagcca gaccatagag
      901 aatgttcgcc aagaggccaa gctcttcgcc atgctgaagc accccaacat cattgcccta
      961 agaggggtat gtctgaagga gcccaacctc tgcttggtca tggagtttgc tcgtggagga
     1021 cctttgaata gagtgttatc tgggaaaagg attcccccag acatcctggt gaattgggct
     1081 gtgcagattg ccagagggat gaactactta catgatgagg caattgttcc catcatccac
     1141 cgcgacctta agtccagcaa catattgatc ctccagaagg tggagaatgg agacctgagc
     1201 aacaagattc tgaagatcac tgattttggc ctggctcggg aatggcaccg aaccaccaag
     1261 atgagtgcgg cagggacgta tgcttggatg gcacccgaag tcatccgggc ctccatgttt
     1321 tccaaaggca gtgatgtgtg gagctatggg gtgctacttt gggagttgct gactggtgag
     1381 gtgccctttc gaggcattga tggcttagca gtcgcttatg gagtggccat gaacaaactc
     1441 gcccttccta ttccttctac gtgcccagaa ccttttgcca aactcatgga agactgctgg
     1501 aatcctgatc cccactcacg accatctttc acgaatatcc tggaccagct aaccaccata
     1561 gaggagtctg gtttctttga aatgcccaag gactccttcc actgcctgca ggacaactgg
     1621 aaacacgaga ttcaggagat gtttgaccaa ctcagggcca aagaaaagga acttcgcacc
     1681 tgggaggagg agctgacgcg ggctgcactg cagcagaaga accaggagga actgctgcgg
     1741 cgtcgggagc aggagctggc cgagcgggag attgacatcc tggaacggga gctcaacatc
     1801 atcatccacc agctgtgcca ggagaagccc cgggtgaaga aacgcaaggg caagttcagg
     1861 aagagccggc tgaagctcaa ggatggcaac cgcatcagcc tcccttctga tttccagcac
     1921 aagttcacgg tgcaggcctc ccctaccatg gataaaagga agagtcttat caacagccgc
     1981 tccagtcctc ctgcaagccc caccatcatt cctcgccttc gagccatcca gttgacacca
     2041 ggtgaaagca gcaaaacctg gggcaggagc tcagtcgtcc caaaggagga aggggaggag
     2101 gaggagaaga gggccccaaa gaagaaggga cggacgtggg ggccagggac gcttggtcag
     2161 aaggagcttg cctcgggaga tgaaggatcc cctcagagac gtgagaaagc taatggttta
     2221 agtaccccat cagaatctcc acatttccac ttgggcctca agtccctggt agatggatat
     2281 aagcagtggt cgtccagtgc ccccaacctg gtgaagggcc caaggagtag cccggccctg
     2341 ccagggttca ccagccttat ggagatggag gatgaggaca gtgaaggccc agggagtgga
     2401 gagagtcgcc tacagcattc acccagccag tcctacctct gtatcccatt ccctcgtgga
     2461 gaggatggcg atggcccctc cagtgatgga atccatgagg agcccacccc agtcaactcg
     2521 gccacgagta cccctcagct gacgccaacc aacagcctca agcggggcgg tgcccaccac
     2581 cgccgctgcg aggtggctct gctcggctgt ggggctgttc tggcagccac aggcctaggg
     2641 tttgacttgc tggaagctgg caagtgccag ctgcttcccc tggaggagcc tgagccacca
     2701 gcccgggagg agaagaaaag acgggagggt ctttttcaga ggtccagccg tcctcgtcgg
     2761 agcaccagcc ccccatcccg aaagcttttc aagaaggagg agcccatgct gttgctagga
     2821 gacccctctg cctccctgac gctgctctcc ctctcctcca tctccgagtg caactccaca
     2881 cgctccctgc tgcgctccga cagcgatgaa attgtcgtgt atgagatgcc agtcagccca
     2941 gtcgaggccc ctcccctgag tccatgtacc cacaaccccc tggtcaatgt ccgagtagag
     3001 cgcttcaaac gagatcctaa ccaatctctg actcccaccc atgtcaccct caccaccccc
     3061 tcgcagccca gcagtcaccg gcggactcct tctgatgggg cccttaagcc agagactctc
     3121 ctagccagca ggagcccctc cagcaatggg ttgagcccca gtcctggagc aggaatgttg
     3181 aaaaccccca gtcccagccg agacccaggt gaattccccc gtctccctga ccccaatgtg
     3241 gtcttccccc caaccccaag gcgctggaac actcagcagg actctacctt ggagagaccc
     3301 aagactctgg agtttctgcc tcggccgcgt ccttctgcca accggcaacg gctggaccct
     3361 tggtggtttg tgtcccccag ccatgcccgc agcacctccc cagccaacag ctccagcaca
     3421 gagacgccca gcaacctgga ctcctgcttt gctagcagta gcagcactgt agaggagcgg
     3481 cctggacttc cagccctgct cccgttccag gcagggccgc tgcccccgac tgagcggacg
     3541 ctcctggacc tggatgcaga ggggcagagt caggacagca ccgtgccgct gtgcagagcg
     3601 gaactgaaca cacacaggcc tgccccttat gagatccagc aggagttctg gtcttagcac
     3661 gaaaaggatt ggggcgggca agggggacag ccagcggaga tgaggggagc tggcgggcac
     3721 agccctttct cagggttgga ccccctgaga tccagcccta cttcttgcac tgataatgca
     3781 ctttgaagat ggaagggatg gaaacagggc cacttcagag ggtctcctgc cctgcagggc
     3841 ctttctaccc gtgtccactg gaggggctgt ggccatcagc tctggctgtg taggggagga
     3901 aggggtgcat gcatgtcccc caccctccac agtcttcctt gcctttagag tgaccctgca
     3961 gagtcactca gccaaatctg tctgctgctc cctctcctca gccagttggg tgtgcgcaga
     4021 gctgtcatag ggtccctttg tcagccccga gttcagcttc ccaaacacca gtgttggata
     4081 ttctgtgatt gattttggtc ctcctccgct gtcccccaac acccaggaat gggaatctgg
     4141 cttggttcga gataggagct tttctgtgtc ctaagccctt tcatgctagc aggaagactg
     4201 aaagcaaggt ggcccagtgt ggggtcatag ggcttgatag acctggcact gcctatctgc
     4261 acttccaggt gccccaccta tttatctgag cccacaggtg gaaaggggaa ctgcctcagt
     4321 gagaacgggg ggacggggat gttaggaaaa atacagtaaa gttgcaatga agaggttcat
     4381 gaagtatgtc cttgttcttt ttggaaactc tcggcaaagg gcaaaccagc aagtattgag
     4441 ggtacccatc tagctacttg gggtcaggac ctcgtcagac caggttcgga tacaatcatc
     4501 tgctcatccc aggaatagtt tcttggggga ctcactcact ggtgccagtt ctaagtcaga
     4561 gacaaaattc cactgtctgt tccttttgct gtctgaactt tatgtgttac tcccttcctt
     4621 tggtcttcac tctaatccct ggagtttgtg ggcttttggt tatgtttggt tagtagatat
     4681 caccgcaatg ccctagaaca gctatgaagc agaataccat atggccacct ggacattggg
     4741 acttgggaat tcactctcaa ctgggccatc catgttgtga tgcccttgaa gtaaaatgga
     4801 gccagcagga gtaccttctg taaatgcatg tggcaaagtg ctatttatag ggtgcccagg
     4861 gagccgctga tgtacaataa ccttgaggtc ccccatactg aaaactgacc aaggcctgtg
     4921 cacaggtagc ccctcatgct gggctctgga ccatgagctg agtaggaagg atagcagagg
     4981 ccaaccctga ccttcctgga agttgtttcc ttaacttgaa tgttgagctt cctctaaagc
     5041 tttctcgtgt atgtcttctc catgccacta ctctgaggcc tcctgtgtta tgtgtgaaca
     5101 gttgtcttta tgtgggaatg acgacttgat tgggagtaga gtctcaaggt cattcccctc
     5161 ttccctcaag actctctgaa tgctgctcca ctgtcttttg tcttggaggt cactcagcag
     5221 gttccttgca tttgctgcct ggatgtgcag ctggcaacag tgatgaattg gtcactgctc
     5281 tttctctata actgggatag atgtcctgcc ttggggtcac taaaggggtg accttgttcc
     5341 ttgctttatg agcccattag cactttggtt caaggggccc accaagtctt ggacgggaag
     5401 gcgctactgg ttttattgcc caaggttttg ttattgcttc tcttctgtgt ccttctcttt
     5461 gttcagtgaa gccaatatgt aagatactgt ttttgtcccc attcccctac tcctgagcta
     5521 ggaggaaaaa atgtgaatct taccagcagt tccagccaac caagtgattc ttcttcattc
     5581 ttgatgggga gaagtacata caaagtttgt tctgacaggg cgcggtggct cacgcctgta
     5641 atcccagcgc tttgggaggc agaggcaggt ggatcacctg aggtcgggag ttcgagacca
     5701 gcctgaccaa catggagata tcctgtctct actaaaaata caaaaaaatt agccaggcat
     5761 ggtggcacgt gcctgtaatc ccagctactc gcaaggctga ggcaggagaa tcgcttgaac
     5821 ctgggaggcg gaggttgcag tgagccaaga ttgcgccatt gcactccagc ctgggcaaca
     5881 agagagaaac tctgtctcaa aaaaaaaaaa aaaaaaaaaa aaagtctgtt ctgtggcctc
     5941 cttcccccgg gccctccata acctcaggct aaaccatgag cacatctgta tatccttggc
     6001 tccactaaat ggccctcttg tggccctcca aaccatgctg cctatacaca gtgctctgtg
     6061 gtatgaagca gagatttttc ctttgctcat ttttaacttt tgccattgtg gtaggttgca
     6121 gccatctttc tagatgtagt gtaactgatg tagtgtaact gaatgatggt cacctgccat
     6181 tgagtgagag gttgagtgcc atccttactt gtccactgtg gggaaagata aagcaagcct
     6241 gggtctttgc taaggaaata attgtacctg aaagaagcaa aagcagggct atccaaaggt
     6301 gctactgtga gcatccatga agaaggaaca gttaccatct ctcagctcct acacacctgg
     6361 tctctgatgg gcccctgact gctgcactct ctcagtgggg caaggttgga gatgggctaa
     6421 ggatctgtat agtagtagca ggtctcagtg gctttctatt cgctcaggtt cccttgttct
     6481 gtggtcctgg tcgggagcag gccctggagc tgtggatgaa gccacttgag tggcagtagc
     6541 cggcccccct ttcctctgcc ttccctttgc acaaagacac gttccttctg ctcttggcag
     6601 gtgctaactt catggaagat ggtaatagga acagatactg ctctctgccc ctgcttcttg
     6661 gattcttcag ctcctaccgc tgattcggat tatccccttt tgaccctctt ggggatccag
     6721 actgctgcca ggaggctgag tagatacctc agttatctgt atgttatttc tgagaggctt
     6781 tggcggatcc tcttcacttg gcatcctgtg gcatccctag ggtctggtgg tagcagtaat
     6841 agcaacaatg atatcttaca tctggacaac tcagaatagt gctaactttt cctcctgacc
     6901 tttgcaaaat gacatcattt tccttgtaca tttgataccc ttgctagtag agaaggggaa
     6961 atgcttaaag atgaaaattc aagtgaagag aaatgaaaaa gtggcttgga ctaattgctg
     7021 agtgaggcca ctgggctcag aactgaacag gttggctgca ttccccattc tgcttttgct
     7081 gtgtggccat aggcttgtct ctttagcccc tctcagattt ccttccttta aaagcaatca
     7141 tcacatgctt tcctctcaag ggagttgggt gtatgtatca gagaaggtca gtgaaacacc
     7201 atagctcctt gccagctcct agaggaagct tctttcatct atacaggatg aatcgctgaa
     7261 gttgagagtg tggctctcat cacctttgcc ttgagggaac ttgccttttc tttcgtcctt
     7321 tggcttctgg aagaggagtg atttggaggt ggttgagcat gactaatggg ggccctggtc
     7381 gggcccatgc ccttctgccg catttatata gcatctggtg ttgacaagca ttctttccta
     7441 attcccccac ttaacttgtc tgatgctgtc tgccaatgtc atcctcacca cttgtgtctt
     7501 acagagaagg aaatgggaga gggaggttgt gtcttacaga gaaggaaatg agagagggag
     7561 gttggtagca acatcagagt gacagttggc tgtctctcat ttcttggggt catcagtctg
     7621 atttgtttag agcctgggat catcccagtt cctggaagaa tctttggaaa agggtcccct
     7681 tgttcttggg acatgtgtca tggtcactaa gccccctttc cctcaggcta ctgttgctca
     7741 gggacacaat gagatacccc aaggacatct agacctgact tttcatgaac tctcttgcct
     7801 ctgtggtccc cacattggag acctccctcc ctcctccttc ccttgtgtga aggagacacc
     7861 tcccgagcaa tcctaactca tccagctcac ttttaacaaa gcaaagagca gagggcactg
     7921 aagactggat ggctgtgaat ggtacacctt ggggttgaaa ccgtgttggc aggaacctgg
     7981 tgataaaagc tgcctacttc ctgggtgtgt gaatttgcac atatcttttc ccctcactgg
     8041 acttcagaag cctactgtga actggggacg atgctatcta ctttccctcc tgaagccctt
     8101 ctaactttca aatgtatggt cctggggcca tgagtcctgc acagaaactg cagccttgcc
     8161 agattgcttc ccttggtgca gaaaagtgtg tgtgtgtgtg tgtgtgaaat atacgtacgg
     8221 ttttacgtca aaaacagtcg aatatcagct atttcatatg gttcacccta atgtacctgc
     8281 ctctctcttt ggctttaggt ctgagaatga cttgtctttg tcaaggtata ctattgttag
     8341 aaacgcatta ccaaatgcat ctcttctgtc ggatcagcgt attcctagat taggaattca
     8401 aattaatgaa aattcacata tgaaaggaaa atccattgct atttctggag aggacctcag
     8461 tcctgggctt ttccctggca ttgctacctg ggtgggtgct caccactcag gtgctggtgt
     8521 tggaaggcag gaggaggaag ctgaaatcct gccgattaag gctaattaac agggtttagg
     8581 tgcctaatta tcatgactca gcccgggact tatggttagc cgtgcaggcc aggtgagtct
     8641 cttatggact tcctctcaga ctgctctttc tcattttgtc ctgatgagat attgacagtc
     8701 atgtccaccc gcttcctcat ccatttcccg tcttgggccc tggaagtacg ggggcctctg
     8761 taggctgcct agggagccct ggctttgctc ttcgtgttgg gctcactcca tgatcaggag
     8821 ccggtgggac tggtccttcc tgattcttac tgtctgtggt tccccatccc ctacggggag
     8881 cctgctttgg gccttgagct ggatagagag aagagctttg gggcccagct ggttatagga
     8941 gctgagcttt tccacacctc tctttgttaa cccttggaaa cagacctgcc tttcacctga
     9001 cccatcttcc tacctgtctg gtctgacctg ccctctttga aagcactcat cacctagttt
     9061 tactaggctg attggcagat gtggacatga caggtgtcta tcgagatagg tgtctaacta
     9121 gttaggtgtc tcaggattgg acagcagaat accattccag gggtgcacag acaggcctct
     9181 cctaccggaa catgagggat agacgtctgg gcattctgaa cccagaggtc agagtagtca
     9241 caagcggagc cctggggagc gagggcccca gggccgtggt gttccttgcc ctgcgctcac
     9301 tgaagtccaa ggccaggttt cagaaatagt gtgctgcctg ttcctgagat ccttcacacc
     9361 tggacaccaa cccagacaaa gcctgactta aaattttgat actgtattca tcgtggaatt
     9421 tttcaataac tctgattttt aaaaaatact gcattgcaat atgatttacc ttgattactg
     9481 aggctctttt ttttttggca cccctttaaa ttttaaccca aggtgagggc ctcactccac
     9541 tctataccca gccctgcctg cccctcacct ggacctgtga gaggggctta ggtaccactg
     9601 tgaaatacgt tttaaatttt tacttgccct tcccctcagg tcctgagtga ggcagtggct
     9661 ctctggcggt gctcgcattt aaataggagt gtgtaggctt acagcaatga aacatctagg
     9721 agcttttaac tttggatcta tacctgggtg tgacatttcc ttggtgttct ctggctgcct
     9781 ttctggctct gcagccctga gggcacttgt gtgtgtgtgt gttctctgga gaagggaagt
     9841 gattatggca gagaggctcc tttagattct cctcttaaac ctctttggaa catgtttgaa
     9901 ttccagaagt gaatgaactt cattcattct ctcgtccaga tttcagaagg gactaaagtg
     9961 aacggaggct ttttcactcc ctggcatgct aagagccaca ttccctagct ctgtgcctgc
    10021 acagtgagtc ttcagaattt ggcccatcac accctctgct agtatcgttt ccaccaccct
    10081 cctcatcctc tgtcatcttt atttcattct catcgtttat ctctacctcc agttcagatg
    10141 ccatgctggc tgtggctctt ttcttcatca ccatcagagt gaggcaaaga tgtatcctgg
    10201 cctagttata aagacgaata atacatgata agaaatcatt aatttttttc cacgtggggg
    10261 gcggtgctgt cctagtgatt catatatata taatttttga ctccttacaa taattctggg
    10321 atgtgggtat tacccccatt taagaatgtg gaaaccaagg ctctgatggc tctgtaattt
    10381 gcccagggtc acacagctag gaagcaagtt gctgatctgc ttggttccaa agtcacctct
    10441 ctttttcctc tgagcacatt tctaagccac tacttagaag ctcttgagat aaagttggcc
    10501 tagctcaggt ccaccgaggt tttgagattg ccctttgccc aaggaggagt tgtgtccttg
    10561 gctcacctgt catctgcctg tgactggact tgaaccctcg cacgtctcag ctgacatcct
    10621 tgatgctgct ggctgccttc ctcgccctgt ttcctctcca tgactccagg ggtttgaagc
    10681 acacaggagc tggacatgtc aattctgtag ctcttctccc aataccactg aaggccgtga
    10741 gcctctctcc tgtttccagc ctgcaggtgc cctgttgctg ctcttcattc cagcttctcc
    10801 tcacttttct ctcagtctct tgagcttgga agccttattg tagcttgtgt ctcctccctg
    10861 ggcacttgag gtcaggcttt tgcctttttg tcacattgag ccacatgcct ttgatacaca
    10921 gttgtagcaa agaagggagg tgatgaactt gctcactttc ttttctgatt tccctcccta
    10981 ctcatcctgc actccccacc gaaacccaga tatcttatag tctaaggctt gtagaggatt
    11041 aaggaaagga attggagatg ggttttactt agttcacaga aaagctttct ttgggatttt
    11101 tcctccccct tagggctttt aagtctaggt gaagtgaaag ttcacacatg tgtttgtttg
    11161 gttgctctgt aattagctac tagtttttat ccctagacct tctctgctcc agtgtcttgt
    11221 tcatgtgtcc tgaccccgtg tccttgaatt cccactttgc tttgggattt aagttattgt
    11281 atgttgtcaa caatatttaa agatgaaaaa gtcctgaagg aaacttacca ggttctttcc
    11341 tttggctttt tttttttttt ctttcgaggt actgtaaatt gttaactagg gatgccaagc
    11401 aggcttggtt caatggctaa acctcttatt gtattacagt gtaatgctga tctcagcctg
    11461 gtctcaatgc cagagcacac agagacttga ataaaactgt tataacgatt a
//
