LOCUS       NM_005923               5157 bp    mRNA    linear   PRI 03-SEP-2024
DEFINITION  Homo sapiens mitogen-activated protein kinase kinase kinase 5
            (MAP3K5), mRNA.
ACCESSION   NM_005923
VERSION     NM_005923.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5157)
  AUTHORS   Zou,M., Chen,W., Li,J., Qi,X., Wang,X., Liu,F., Hu,J. and Zhang,Q.
  TITLE     Apoptosis Signal-Regulated Kinase-1 Promotes Nucleus Pulposus Cell
            Senescence and Apoptosis to Regulate Intervertebral Disc
            Degeneration
  JOURNAL   Am J Pathol 194 (9), 1737-1751 (2024)
   PUBMED   38879082
  REMARK    GeneRIF: Apoptosis Signal-Regulated Kinase-1 Promotes Nucleus
            Pulposus Cell Senescence and Apoptosis to Regulate Intervertebral
            Disc Degeneration.
REFERENCE   2  (bases 1 to 5157)
  AUTHORS   Gao,K., She,K., Fan,J., Shao,L. and Ji,Z.
  TITLE     Multiple Pathway-Dephosphorylated ASK-1 Confers
            Temozolomide-Resistance to Human Glioma Cells
  JOURNAL   Turk Neurosurg 34 (1), 67-73 (2024)
   PUBMED   36951032
  REMARK    GeneRIF: Multiple Pathway-Dephosphorylated ASK-1 Confers
            Temozolomide-Resistance to Human Glioma Cells.
REFERENCE   3  (bases 1 to 5157)
  AUTHORS   Duan,Z., Li,H., Hu,B., Li,Y. and Huang,L.
  TITLE     [Tumor antigen-loaded dendritic cells combined with
            cytokine-induced killer (CIK) enhance the killing activity of human
            esophageal cancer cells by promoting ASK1 activation]
  JOURNAL   Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi 39 (6), 501-508 (2023)
   PUBMED   37340918
  REMARK    GeneRIF: [Tumor antigen-loaded dendritic cells combined with
            cytokine-induced killer (CIK) enhance the killing activity of human
            esophageal cancer cells by promoting ASK1 activation].
REFERENCE   4  (bases 1 to 5157)
  AUTHORS   Giri,H., Srivastava,A.K. and Naik,U.P.
  TITLE     Apoptosis signal-regulating kinase-1 regulates thrombin-induced
            endothelial permeability
  JOURNAL   Vascul Pharmacol 145, 107088 (2022)
   PUBMED   35798237
  REMARK    GeneRIF: Apoptosis signal-regulating kinase-1 regulates
            thrombin-induced endothelial permeability.
REFERENCE   5  (bases 1 to 5157)
  AUTHORS   Shen,W.B., Elahi,M., Wang,B., Zhan,M., Yang,P. and Yang,P.
  TITLE     Oxidative Stress Kinase Activation and Impaired Insulin Receptor
            Signaling Precede Overt Alzheimer's Disease Neuropathology
  JOURNAL   J Alzheimers Dis 90 (2), 841-857 (2022)
   PUBMED   36189587
  REMARK    GeneRIF: Oxidative Stress Kinase Activation and Impaired Insulin
            Receptor Signaling Precede Overt Alzheimer's Disease
            Neuropathology.
REFERENCE   6  (bases 1 to 5157)
  AUTHORS   Gotoh,Y. and Cooper,J.A.
  TITLE     Reactive oxygen species- and dimerization-induced activation of
            apoptosis signal-regulating kinase 1 in tumor necrosis factor-alpha
            signal transduction
  JOURNAL   J Biol Chem 273 (28), 17477-17482 (1998)
   PUBMED   9651337
REFERENCE   7  (bases 1 to 5157)
  AUTHORS   Saitoh,M., Nishitoh,H., Fujii,M., Takeda,K., Tobiume,K., Sawada,Y.,
            Kawabata,M., Miyazono,K. and Ichijo,H.
  TITLE     Mammalian thioredoxin is a direct inhibitor of apoptosis
            signal-regulating kinase (ASK) 1
  JOURNAL   EMBO J 17 (9), 2596-2606 (1998)
   PUBMED   9564042
REFERENCE   8  (bases 1 to 5157)
  AUTHORS   Ichijo,H., Nishida,E., Irie,K., ten Dijke,P., Saitoh,M.,
            Moriguchi,T., Takagi,M., Matsumoto,K., Miyazono,K. and Gotoh,Y.
  TITLE     Induction of apoptosis by ASK1, a mammalian MAPKKK that activates
            SAPK/JNK and p38 signaling pathways
  JOURNAL   Science 275 (5296), 90-94 (1997)
   PUBMED   8974401
REFERENCE   9  (bases 1 to 5157)
  AUTHORS   Rampoldi,L., Zimbello,R., Bortoluzzi,S., Tiso,N., Valle,G.,
            Lanfranchi,G. and Danieli,G.A.
  TITLE     Chromosomal localization of four MAPK signaling cascade genes:
            MEK1, MEK3, MEK4 and MEKK5
  JOURNAL   Cytogenet Cell Genet 78 (3-4), 301-303 (1997)
   PUBMED   9465908
REFERENCE   10 (bases 1 to 5157)
  AUTHORS   Wang,X.S., Diener,K., Jannuzzi,D., Trollinger,D., Tan,T.H.,
            Lichenstein,H., Zukowski,M. and Yao,Z.
  TITLE     Molecular cloning and characterization of a novel protein kinase
            with a catalytic domain homologous to mitogen-activated protein
            kinase kinase kinase
  JOURNAL   J Biol Chem 271 (49), 31607-31611 (1996)
   PUBMED   8940179
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL121933.15 and AL024508.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 26, 2018 this sequence version replaced NM_005923.3.
            
            Summary: Mitogen-activated protein kinase (MAPK) signaling cascades
            include MAPK or extracellular signal-regulated kinase (ERK), MAPK
            kinase (MKK or MEK), and MAPK kinase kinase (MAPKKK or MEKK). MAPKK
            kinase/MEKK phosphorylates and activates its downstream protein
            kinase, MAPK kinase/MEK, which in turn activates MAPK. The kinases
            of these signaling cascades are highly conserved, and homologs
            exist in yeast, Drosophila, and mammalian cells. MAPKKK5 contains
            1,374 amino acids with all 11 kinase subdomains. Northern blot
            analysis shows that MAPKKK5 transcript is abundantly expressed in
            human heart and pancreas. The MAPKKK5 protein phosphorylates and
            activates MKK4 (aliases SERK1, MAPKK4) in vitro, and activates
            c-Jun N-terminal kinase (JNK)/stress-activated protein kinase
            (SAPK) during transient expression in COS and 293 cells; MAPKKK5
            does not activate MAPK/ERK. [provided by RefSeq, Jul 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC088829.1, U67156.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000359015.5/ ENSP00000351908.4
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-768               AL121933.15        107665-108432       c
            769-908             AL121933.15        36405-36544         c
            909-932             AL121933.15        21065-21088         c
            933-1126            AL121933.15        14444-14637         c
            1127-1295           AL121933.15        13174-13342         c
            1296-1402           AL121933.15        11906-12012         c
            1403-1573           AL121933.15        10095-10265         c
            1574-1686           AL024508.1         139926-140038       c
            1687-1846           AL024508.1         129862-130021       c
            1847-2000           AL024508.1         126950-127103       c
            2001-2108           AL024508.1         121627-121734       c
            2109-2158           AL024508.1         113163-113212       c
            2159-2254           AL024508.1         110186-110281       c
            2255-2336           AL024508.1         107968-108049       c
            2337-2470           AL024508.1         93491-93624         c
            2471-2598           AL024508.1         84802-84929         c
            2599-2735           AL024508.1         83763-83899         c
            2736-2841           AL024508.1         81925-82030         c
            2842-2999           AL024508.1         75852-76009         c
            3000-3177           AL024508.1         72445-72622         c
            3178-3198           AL024508.1         71665-71685         c
            3199-3376           AL024508.1         63080-63257         c
            3377-3545           AL024508.1         62816-62984         c
            3546-3731           AL024508.1         54198-54383         c
            3732-3837           AL024508.1         50944-51049         c
            3838-4081           AL024508.1         38274-38517         c
            4082-4194           AL024508.1         33146-33258         c
            4195-4307           AL024508.1         32176-32288         c
            4308-4384           AL024508.1         29443-29519         c
            4385-5157           AL024508.1         27689-28461         c
FEATURES             Location/Qualifiers
     source          1..5157
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="6"
                     /map="6q23.3"
     gene            1..5157
                     /gene="MAP3K5"
                     /gene_synonym="ASK1; MAPKKK5; MEKK5"
                     /note="mitogen-activated protein kinase kinase kinase 5"
                     /db_xref="GeneID:4217"
                     /db_xref="HGNC:HGNC:6857"
                     /db_xref="MIM:602448"
     exon            1..768
                     /gene="MAP3K5"
                     /gene_synonym="ASK1; MAPKKK5; MEKK5"
                     /inference="alignment:Splign:2.1.0"
     CDS             321..4445
                     /gene="MAP3K5"
                     /gene_synonym="ASK1; MAPKKK5; MEKK5"
                     /EC_number="2.7.11.25"
                     /note="MAP/ERK kinase kinase 5; MAPK/ERK kinase kinase 5;
                     ASK-1; MEKK 5; MEK kinase 5; apoptosis signal-regulating
                     kinase 1"
                     /codon_start=1
                     /product="mitogen-activated protein kinase kinase kinase
                     5"
                     /protein_id="NP_005914.1"
                     /db_xref="CCDS:CCDS5179.1"
                     /db_xref="GeneID:4217"
                     /db_xref="HGNC:HGNC:6857"
                     /db_xref="MIM:602448"
                     /translation="MSTEADEGITFSVPPFAPSGFCTIPEGGICRRGGAAAVGEGEEH
                     QLPPPPPGSFWNVESAAAPGIGCPAATSSSSATRGRGSSVGGGSRRTTVAYVINEASQ
                     GQLVVAESEALQSLREACETVGATLETLHFGKLDFGETTVLDRFYNADIAVVEMSDAF
                     RQPSLFYHLGVRESFSMANNIILYCDTNSDSLQSLKEIICQKNTMCTGNYTFVPYMIT
                     PHNKVYCCDSSFMKGLTELMQPNFELLLGPICLPLVDRFIQLLKVAQASSSQYFRESI
                     LNDIRKARNLYTGKELAAELARIRQRVDNIEVLTADIVINLLLSYRDIQDYDSIVKLV
                     ETLEKLPTFDLASHHHVKFHYAFALNRRNLPGDRAKALDIMIPMVQSEGQVASDMYCL
                     VGRIYKDMFLDSNFTDTESRDHGASWFKKAFESEPTLQSGINYAVLLLAAGHQFESSF
                     ELRKVGVKLSSLLGKKGNLEKLQSYWEVGFFLGASVLANDHMRVIQASEKLFKLKTPA
                     WYLKSIVETILIYKHFVKLTTEQPVAKQELVDFWMDFLVEATKTDVTVVRFPVLILEP
                     TKIYQPSYLSINNEVEEKTISIWHVLPDDKKGIHEWNFSASSVRGVSISKFEERCCFL
                     YVLHNSDDFQIYFCTELHCKKFFEMVNTITEEKGRSTEEGDCESDLLEYDYEYDENGD
                     RVVLGKGTYGIVYAGRDLSNQVRIAIKEIPERDSRYSQPLHEEIALHKHLKHKNIVQY
                     LGSFSENGFIKIFMEQVPGGSLSALLRSKWGPLKDNEQTIGFYTKQILEGLKYLHDNQ
                     IVHRDIKGDNVLINTYSGVLKISDFGTSKRLAGINPCTETFTGTLQYMAPEIIDKGPR
                     GYGKAADIWSLGCTIIEMATGKPPFYELGEPQAAMFKVGMFKVHPEIPESMSAEAKAF
                     ILKCFEPDPDKRACANDLLVDEFLKVSSKKKKTQPKLSALSAGSNEYLRSISLPVPVL
                     VEDTSSSSEYGSVSPDTELKVDPFSFKTRAKSCGERDVKGIRTLFLGIPDENFEDHSA
                     PPSPEEKDSGFFMLRKDSERRATLHRILTEDQDKIVRNLMESLAQGAEEPKLKWEHIT
                     TLIASLREFVRSTDRKIIATTLSKLKLELDFDSHGISQVQVVLFGFQDAVNKVLRNHN
                     IKPHWMFALDSIIRKAVQTAITILVPELRPHFSLASESDTADQEDLDVEDDHEEQPSN
                     QTVRRPQAVIEDAVATSGVSTLSSTVSHDSQSAHRSLNVQLGRMKIETNRLLEELVRK
                     EKELQALLHRAIEEKDQEIKHLKLKSQPIEIPELPVFHLNSSGTNTEDSELTDWLRVN
                     GADEDTISRFLAEDYTLLDVLYYVTRDDLKCLRLRGGMLCTLWKAIIDFRNKQT"
     exon            769..908
                     /gene="MAP3K5"
                     /gene_synonym="ASK1; MAPKKK5; MEKK5"
                     /inference="alignment:Splign:2.1.0"
     exon            909..932
                     /gene="MAP3K5"
                     /gene_synonym="ASK1; MAPKKK5; MEKK5"
                     /inference="alignment:Splign:2.1.0"
     exon            933..1126
                     /gene="MAP3K5"
                     /gene_synonym="ASK1; MAPKKK5; MEKK5"
                     /inference="alignment:Splign:2.1.0"
     exon            1127..1295
                     /gene="MAP3K5"
                     /gene_synonym="ASK1; MAPKKK5; MEKK5"
                     /inference="alignment:Splign:2.1.0"
     exon            1296..1402
                     /gene="MAP3K5"
                     /gene_synonym="ASK1; MAPKKK5; MEKK5"
                     /inference="alignment:Splign:2.1.0"
     exon            1403..1573
                     /gene="MAP3K5"
                     /gene_synonym="ASK1; MAPKKK5; MEKK5"
                     /inference="alignment:Splign:2.1.0"
     exon            1574..1686
                     /gene="MAP3K5"
                     /gene_synonym="ASK1; MAPKKK5; MEKK5"
                     /inference="alignment:Splign:2.1.0"
     exon            1687..1846
                     /gene="MAP3K5"
                     /gene_synonym="ASK1; MAPKKK5; MEKK5"
                     /inference="alignment:Splign:2.1.0"
     exon            1847..2000
                     /gene="MAP3K5"
                     /gene_synonym="ASK1; MAPKKK5; MEKK5"
                     /inference="alignment:Splign:2.1.0"
     exon            2001..2108
                     /gene="MAP3K5"
                     /gene_synonym="ASK1; MAPKKK5; MEKK5"
                     /inference="alignment:Splign:2.1.0"
     exon            2109..2158
                     /gene="MAP3K5"
                     /gene_synonym="ASK1; MAPKKK5; MEKK5"
                     /inference="alignment:Splign:2.1.0"
     exon            2159..2254
                     /gene="MAP3K5"
                     /gene_synonym="ASK1; MAPKKK5; MEKK5"
                     /inference="alignment:Splign:2.1.0"
     exon            2255..2336
                     /gene="MAP3K5"
                     /gene_synonym="ASK1; MAPKKK5; MEKK5"
                     /inference="alignment:Splign:2.1.0"
     exon            2337..2470
                     /gene="MAP3K5"
                     /gene_synonym="ASK1; MAPKKK5; MEKK5"
                     /inference="alignment:Splign:2.1.0"
     exon            2471..2598
                     /gene="MAP3K5"
                     /gene_synonym="ASK1; MAPKKK5; MEKK5"
                     /inference="alignment:Splign:2.1.0"
     exon            2599..2735
                     /gene="MAP3K5"
                     /gene_synonym="ASK1; MAPKKK5; MEKK5"
                     /inference="alignment:Splign:2.1.0"
     exon            2736..2841
                     /gene="MAP3K5"
                     /gene_synonym="ASK1; MAPKKK5; MEKK5"
                     /inference="alignment:Splign:2.1.0"
     exon            2842..2999
                     /gene="MAP3K5"
                     /gene_synonym="ASK1; MAPKKK5; MEKK5"
                     /inference="alignment:Splign:2.1.0"
     exon            3000..3177
                     /gene="MAP3K5"
                     /gene_synonym="ASK1; MAPKKK5; MEKK5"
                     /inference="alignment:Splign:2.1.0"
     exon            3178..3198
                     /gene="MAP3K5"
                     /gene_synonym="ASK1; MAPKKK5; MEKK5"
                     /inference="alignment:Splign:2.1.0"
     exon            3199..3376
                     /gene="MAP3K5"
                     /gene_synonym="ASK1; MAPKKK5; MEKK5"
                     /inference="alignment:Splign:2.1.0"
     exon            3377..3545
                     /gene="MAP3K5"
                     /gene_synonym="ASK1; MAPKKK5; MEKK5"
                     /inference="alignment:Splign:2.1.0"
     exon            3546..3731
                     /gene="MAP3K5"
                     /gene_synonym="ASK1; MAPKKK5; MEKK5"
                     /inference="alignment:Splign:2.1.0"
     exon            3732..3837
                     /gene="MAP3K5"
                     /gene_synonym="ASK1; MAPKKK5; MEKK5"
                     /inference="alignment:Splign:2.1.0"
     exon            3838..4081
                     /gene="MAP3K5"
                     /gene_synonym="ASK1; MAPKKK5; MEKK5"
                     /inference="alignment:Splign:2.1.0"
     exon            4082..4194
                     /gene="MAP3K5"
                     /gene_synonym="ASK1; MAPKKK5; MEKK5"
                     /inference="alignment:Splign:2.1.0"
     exon            4195..4307
                     /gene="MAP3K5"
                     /gene_synonym="ASK1; MAPKKK5; MEKK5"
                     /inference="alignment:Splign:2.1.0"
     exon            4308..4384
                     /gene="MAP3K5"
                     /gene_synonym="ASK1; MAPKKK5; MEKK5"
                     /inference="alignment:Splign:2.1.0"
     exon            4385..5157
                     /gene="MAP3K5"
                     /gene_synonym="ASK1; MAPKKK5; MEKK5"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5136..5141
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MAP3K5"
                     /gene_synonym="ASK1; MAPKKK5; MEKK5"
                     /note="hexamer: ATTAAA"
     polyA_site      5157
                     /gene="MAP3K5"
                     /gene_synonym="ASK1; MAPKKK5; MEKK5"
                     /note="major polyA site"
ORIGIN      
        1 gagccgactt gtcagccggc caagaaaagg aagctccgtc ccttcccgct cacccggctt
       61 ccccacccct tgtactctaa actctgcaga gggcgagcgg cgcggccacg gaggcgccga
      121 ggaggagcga gccgccgccg ggcagcggcg tgccctcggg ggagagggcg ccggagagga
      181 ggcggcggcg cggcggcgag ggcgcggcgc gcgatggcag ctgcttagcc cggcgggcgc
      241 ggagcagccc cgagctgtgg ctggccaggc ggtgcggctg ggcgggggac gccgccgccg
      301 ttgctgcccg gcccggagag atgagcacgg aggcggacga gggcatcact ttctctgtgc
      361 cacccttcgc cccctcgggc ttctgcacca tccccgaggg cggcatctgc aggaggggag
      421 gagcggcggc ggtgggcgag ggcgaggagc accagctgcc accgccgccg ccgggcagct
      481 tctggaacgt ggagagcgcc gctgcccctg gcatcggttg tccggcggcc acctcctcga
      541 gcagtgccac ccgaggccgg ggcagctctg ttggcggggg cagccgacgg accacggtgg
      601 catatgtgat caacgaagcg agccaagggc aactggtggt ggccgagagc gaggccctgc
      661 agagcttgcg ggaggcgtgc gagacagtgg gcgccaccct ggaaaccctg cattttggga
      721 aactcgactt tggagaaacc accgtgctgg accgctttta caatgcagat attgcggtgg
      781 tggagatgag cgatgccttc cggcagccgt ccttgtttta ccaccttggg gtgagagaaa
      841 gtttcagcat ggccaacaac atcatcctct actgtgatac taactcggac tctctgcagt
      901 cactgaagga aataatttgc cagaagaata ctatgtgcac tgggaactac acctttgttc
      961 cttacatgat aactccacat aacaaagtct actgctgtga cagcagcttc atgaaggggt
     1021 tgacagagct catgcaaccg aacttcgagc tgcttcttgg acccatctgc ttacctcttg
     1081 tggatcgttt tattcaactt ttgaaggtgg cacaagcaag ttctagccag tacttccggg
     1141 aatctatact caatgacatc aggaaagctc gtaatttata cactggtaaa gaattggcag
     1201 ctgagttggc aagaattcgg cagcgagtag ataatatcga agtcttgaca gcagatattg
     1261 tcataaatct gttactttcc tacagagata tccaggacta tgattctatt gtgaagctgg
     1321 tagagacttt agaaaaactg ccaacctttg atttggcctc ccatcaccat gtgaagtttc
     1381 attatgcatt tgcactgaat aggagaaatc tccctggtga cagagcaaaa gctcttgata
     1441 ttatgattcc catggtgcaa agcgaaggac aagttgcttc agatatgtat tgcctagttg
     1501 gtcgaatcta caaagatatg tttttggact ctaatttcac ggacactgaa agcagagacc
     1561 atggagcttc ttggttcaaa aaggcatttg aatctgagcc aacactacag tcaggaatta
     1621 attatgcggt cctcctcctg gcagctggac accagtttga atcttccttt gagctccgga
     1681 aagttggggt gaagctaagt agtcttcttg gtaaaaaggg aaacttggaa aaactccaga
     1741 gctactggga agttggattt tttctggggg ccagcgtcct agccaatgac cacatgagag
     1801 tcattcaagc atctgaaaag ctttttaaac tgaagacacc agcatggtac ctcaagtcta
     1861 ttgtagagac aattttaata tataagcatt ttgtgaaact gaccacagaa cagcctgtgg
     1921 ccaagcaaga acttgtggac ttttggatgg atttcctggt cgaggccaca aagacagatg
     1981 ttactgtggt taggtttcca gtattaatat tagaaccaac caaaatctat caaccttctt
     2041 atttgtctat caacaatgaa gttgaggaaa agacaatctc tatttggcac gtgcttcctg
     2101 atgacaagaa aggtatacat gagtggaatt ttagtgcctc ttctgtcagg ggagtgagta
     2161 tttctaaatt tgaagaaaga tgctgctttc tttatgtgct tcacaattct gatgatttcc
     2221 aaatctattt ctgtacagaa cttcattgta aaaagttttt tgagatggtg aacaccatta
     2281 ccgaagagaa ggggagaagc acagaggaag gagactgtga aagtgacttg ctggagtatg
     2341 actatgaata tgatgaaaat ggtgacagag tcgttttagg aaaaggcact tatgggatag
     2401 tctacgcagg tcgggacttg agcaaccaag tcagaattgc tattaaggaa atcccagaga
     2461 gagacagcag atactctcag cccctgcatg aagaaatagc attgcataaa cacctgaagc
     2521 acaaaaatat tgtccagtat ctgggctctt tcagtgagaa tggtttcatt aaaatcttca
     2581 tggagcaggt ccctggagga agtctttctg ctctccttcg ttccaaatgg ggtccattaa
     2641 aagacaatga gcaaacaatt ggcttttata caaagcaaat actggaagga ttaaaatatc
     2701 tccatgacaa tcagatagtt caccgggaca taaagggtga caatgtgttg attaatacct
     2761 acagtggtgt tctcaagatc tctgacttcg gaacatcaaa gaggcttgct ggcataaacc
     2821 cctgtactga aacttttact ggtaccctcc agtatatggc accagaaata atagataaag
     2881 gaccaagagg ctacggaaaa gcagcagaca tctggtctct gggctgtaca atcattgaaa
     2941 tggccacagg aaaaccccca ttttatgaac tgggagaacc acaagcagct atgttcaagg
     3001 tgggaatgtt taaagtccac cctgagatcc cagagtccat gtctgcagag gccaaggcat
     3061 tcatactgaa atgttttgaa ccagatcctg acaagagagc ctgtgctaac gacttgcttg
     3121 ttgatgagtt tttaaaagtt tcaagcaaaa agaaaaagac acaacctaag ctttcagctc
     3181 tttcagctgg atcaaatgaa tatctcagga gtatatcctt gccggtacct gtgctggtgg
     3241 aggacaccag cagcagcagt gagtacggct cagtttcacc cgacacggag ttgaaagtgg
     3301 accccttctc tttcaaaaca agagccaagt cctgcggaga aagagatgtc aagggaattc
     3361 ggacactctt tttgggcatt ccagatgaga attttgaaga tcacagtgct cctccttccc
     3421 ctgaagaaaa agattctgga ttcttcatgc tgaggaagga cagtgagagg cgagctaccc
     3481 ttcacaggat cctgacggaa gaccaagaca aaattgtgag aaacctaatg gaatctttag
     3541 ctcagggggc tgaagaaccg aaactaaaat gggaacacat cacaaccctc attgcaagcc
     3601 tcagagaatt tgtgagatcc actgaccgaa aaatcatagc caccacactg tcaaagctga
     3661 aactggagct ggacttcgac agccatggca ttagccaagt ccaggtggta ctctttggtt
     3721 ttcaagatgc tgtcaataaa gttcttcgga atcataacat caagccgcac tggatgtttg
     3781 ccttagacag tatcattcgg aaggcggtac agacagccat taccatcctg gttccagaac
     3841 taaggccaca tttcagcctt gcatctgaga gtgatactgc tgatcaagaa gacttggatg
     3901 tagaagatga ccatgaggaa cagccttcaa atcaaactgt ccgaagacct caggctgtca
     3961 ttgaagatgc tgtggctacc tcaggcgtga gcacgctcag ttctactgtg tctcatgatt
     4021 cccagagtgc tcaccggtca ctgaatgtac agcttggaag gatgaaaata gaaaccaata
     4081 gattactgga agaattggtt cggaaagaga aagaattaca agcactcctt catcgagcta
     4141 ttgaagaaaa agaccaagaa attaaacacc tgaagcttaa gtcccaaccc atagaaattc
     4201 ctgaattgcc tgtatttcat ctaaattctt ctggcacaaa tactgaagat tctgaactta
     4261 ccgactggct gagagtgaat ggagctgatg aagacactat aagccggttt ttggctgaag
     4321 attatacact attggatgtt ctctactatg ttacacgtga tgacttaaaa tgcttgagac
     4381 taaggggagg gatgctgtgc acactgtgga aggctatcat tgactttcga aacaaacaga
     4441 cttgactgtt gctcaatcta atcttcgatg gaaattctaa aaattaatac agagctgatc
     4501 ttcttggggg tgggaaaatc gaagggagag gagaaaggcg ctgcacttta aatccagtat
     4561 ttgtttactc atgttaaaaa aaaaaaaaac agacaaaaca cactgaaatt tcctaactac
     4621 atctatttct ataattttta aggactcttc ataaggactc ttaaaataat cctgaacatt
     4681 agaaccctaa tgttcaggaa gattttaatc taagcatttt tatggaaata tttttaatgc
     4741 agcagctatt gcacttcagc caaatgttta tttcacacaa aacggatgta acatttcatg
     4801 tgatcgtgca ccactggaac aaaaccaaaa tgtgaccata actgtttagg cttctgtgtg
     4861 tttgtaatat gctctaataa tctgagtaga aatgcgtaat ttcaattact gtataaagtt
     4921 tatgtttttt taagtgtgca gaatctgaga gcaatggttt ttacttctct gtgttaattg
     4981 taatattgac tctattttgt aacttaagtt tctgacctgt cgtacatttg tttgagtcgt
     5041 ttatgtacta ctgaactgta ccagttgcac atgcttgaac tgtagtaatg ttagcttgtt
     5101 ctaaagctat ccattgtgtc atatttactc taaaaattaa agagactctc aacatac
//
