LOCUS       NM_005922               5500 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens mitogen-activated protein kinase kinase kinase 4
            (MAP3K4), transcript variant 1, mRNA.
ACCESSION   NM_005922
VERSION     NM_005922.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5500)
  AUTHORS   Cui,F., Sun,Z., Zhang,X. and Liu,C.
  TITLE     CircMAP3K4 Suppresses H2O2-Induced Human Lens Epithelial Cell
            Injury by miR-630/ERCC6 Axis in Age-Related Cataract
  JOURNAL   Curr Eye Res 49 (5), 487-495 (2024)
   PUBMED   38152055
  REMARK    GeneRIF: CircMAP3K4 Suppresses H2O2-Induced Human Lens Epithelial
            Cell Injury by miR-630/ERCC6 Axis in Age-Related Cataract.
REFERENCE   2  (bases 1 to 5500)
  AUTHORS   Ma,Y., Liu,Y., Shu,B., Yang,J., Lv,L., Zhou,L., Wang,L. and Shi,Z.
  TITLE     CircMAP3K4 protects human lens epithelial cells from H2O2-induced
            dysfunction by targeting miR-193a-3p/PLCD3 axis in age-related
            cataract
  JOURNAL   Cell Cycle 22 (3), 303-315 (2023)
   PUBMED   36071682
  REMARK    GeneRIF: CircMAP3K4 protects human lens epithelial cells from
            H2O2-induced dysfunction by targeting miR-193a-3p/PLCD3 axis in
            age-related cataract.
REFERENCE   3  (bases 1 to 5500)
  AUTHORS   He,Z., Bin,Y., Chen,G., Li,Q., Fan,W., Ma,Y., Yi,J., Luo,X., Tan,Z.
            and Li,J.
  TITLE     Identification of MAP3K4 as a novel regulation factor of hepatic
            lipid metabolism in non-alcoholic fatty liver disease
  JOURNAL   J Transl Med 20 (1), 529 (2022)
   PUBMED   36376950
  REMARK    GeneRIF: Identification of MAP3K4 as a novel regulation factor of
            hepatic lipid metabolism in non-alcoholic fatty liver disease.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 5500)
  AUTHORS   Sun,Y., Lv,Y., Ren,H.W., Wang,G.Y., Xuan,L.N., Luo,Y.Y. and
            Luan,Z.L.
  TITLE     Association between MAP3K4 gene polymorphisms and the risk of
            schizophrenia susceptibility in a Northeast Chinese Han population
  JOURNAL   Metab Brain Dis 37 (5), 1365-1371 (2022)
   PUBMED   35445959
  REMARK    GeneRIF: Association between MAP3K4 gene polymorphisms and the risk
            of schizophrenia susceptibility in a Northeast Chinese Han
            population.
REFERENCE   5  (bases 1 to 5500)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   6  (bases 1 to 5500)
  AUTHORS   Posas,F. and Saito,H.
  TITLE     Activation of the yeast SSK2 MAP kinase kinase kinase by the SSK1
            two-component response regulator
  JOURNAL   EMBO J 17 (5), 1385-1394 (1998)
   PUBMED   9482735
REFERENCE   7  (bases 1 to 5500)
  AUTHORS   Takekawa,M., Posas,F. and Saito,H.
  TITLE     A human homolog of the yeast Ssk2/Ssk22 MAP kinase kinase kinases,
            MTK1, mediates stress-induced activation of the p38 and JNK
            pathways
  JOURNAL   EMBO J 16 (16), 4973-4982 (1997)
   PUBMED   9305639
REFERENCE   8  (bases 1 to 5500)
  AUTHORS   Fanger,G.R., Johnson,N.L. and Johnson,G.L.
  TITLE     MEK kinases are regulated by EGF and selectively interact with
            Rac/Cdc42
  JOURNAL   EMBO J 16 (16), 4961-4972 (1997)
   PUBMED   9305638
REFERENCE   9  (bases 1 to 5500)
  AUTHORS   Gerwins,P., Blank,J.L. and Johnson,G.L.
  TITLE     Cloning of a novel mitogen-activated protein kinase kinase kinase,
            MEKK4, that selectively regulates the c-Jun amino terminal kinase
            pathway
  JOURNAL   J Biol Chem 272 (13), 8288-8295 (1997)
   PUBMED   9079650
REFERENCE   10 (bases 1 to 5500)
  AUTHORS   Adams,M.D., Kerlavage,A.R., Fleischmann,R.D., Fuldner,R.A.,
            Bult,C.J., Lee,N.H., Kirkness,E.F., Weinstock,K.G., Gocayne,J.D.,
            White,O. et al.
  TITLE     Initial assessment of human gene diversity and expression patterns
            based upon 83 million nucleotides of cDNA sequence
  JOURNAL   Nature 377 (6547 Suppl), 3-174 (1995)
   PUBMED   7566098
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL139393.13, AL596452.2,
            AL591045.5 and AL109942.13.
            
            On Nov 23, 2018 this sequence version replaced NM_005922.3.
            
            Summary: The central core of each mitogen-activated protein kinase
            (MAPK) pathway is a conserved cascade of 3 protein kinases: an
            activated MAPK kinase kinase (MAPKKK) phosphorylates and activates
            a specific MAPK kinase (MAPKK), which then activates a specific
            MAPK. While the ERK MAPKs are activated by mitogenic stimulation,
            the CSBP2 and JNK MAPKs are activated by environmental stresses
            such as osmotic shock, UV irradiation, wound stress, and
            inflammatory factors. This gene encodes a MAPKKK, the MEKK4
            protein, also called MTK1. This protein contains a protein kinase
            catalytic domain at the C terminus. The N-terminal nonkinase domain
            may contain a regulatory domain. Expression of MEKK4 in mammalian
            cells activated the CSBP2 and JNK MAPK pathways, but not the ERK
            pathway. In vitro kinase studies indicated that recombinant MEKK4
            can specifically phosphorylate and activate PRKMK6 and SERK1,
            MAPKKs that activate CSBP2 and JNK, respectively but cannot
            phosphorylate PRKMK1, an MAPKK that activates ERKs. MEKK4 is a
            major mediator of environmental stresses that activate the CSBP2
            MAPK pathway, and a minor mediator of the JNK pathway. Several
            alternatively spliced transcripts encoding distinct isoforms have
            been described. [provided by RefSeq, May 2014].
            
            Transcript Variant: This variant (1) encodes the longest isoform
            (a).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF002715.1, SRR1660803.155161.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1968189
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000392142.9/ ENSP00000375986.4
            RefSeq Select criteria :: based on conservation, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-315               AL139393.13        58928-59242
            316-506             AL596452.2         17794-17984
            507-1870            AL596452.2         32151-33514
            1871-2113           AL596452.2         54143-54385
            2114-2260           AL596452.2         57001-57147
            2261-2418           AL596452.2         64416-64573
            2419-2535           AL596452.2         68036-68152
            2536-2635           AL591045.5         2232-2331
            2636-2719           AL591045.5         2437-2520
            2720-2986           AL591045.5         3541-3807
            2987-3136           AL591045.5         5175-5324
            3137-3298           AL591045.5         7232-7393
            3299-3432           AL591045.5         7863-7996
            3433-3511           AL591045.5         8831-8909
            3512-3590           AL591045.5         9626-9704
            3591-3687           AL591045.5         12933-13029
            3688-3837           AL591045.5         14131-14280
            3838-3938           AL591045.5         17745-17845
            3939-4019           AL591045.5         18552-18632
            4020-4211           AL591045.5         22367-22558
            4212-4282           AL591045.5         23752-23822
            4283-4399           AL591045.5         24596-24712
            4400-4559           AL591045.5         25608-25767
            4560-4682           AL109942.13        2336-2458
            4683-4789           AL109942.13        3168-3274
            4790-4969           AL109942.13        5623-5802
            4970-5500           AL109942.13        7350-7880
FEATURES             Location/Qualifiers
     source          1..5500
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="6"
                     /map="6q26"
     gene            1..5500
                     /gene="MAP3K4"
                     /gene_synonym="MAPKKK4; MEKK 4; MEKK4; MTK1; PRO0412"
                     /note="mitogen-activated protein kinase kinase kinase 4"
                     /db_xref="GeneID:4216"
                     /db_xref="HGNC:HGNC:6856"
                     /db_xref="MIM:602425"
     exon            1..315
                     /gene="MAP3K4"
                     /gene_synonym="MAPKKK4; MEKK 4; MEKK4; MTK1; PRO0412"
                     /inference="alignment:Splign:2.1.0"
     CDS             164..4990
                     /gene="MAP3K4"
                     /gene_synonym="MAPKKK4; MEKK 4; MEKK4; MTK1; PRO0412"
                     /EC_number="2.7.11.25"
                     /note="isoform a is encoded by transcript variant 1;
                     SSK2/SSK22 MAP kinase kinase kinase, yeast, homolog of;
                     MAP/ERK kinase kinase 4; MAPK/ERK kinase kinase 4;
                     dJ473J16.1 (mitogen-activated protein kinase kinase kinase
                     4); predicted protein of HQ0412; MEK kinase 4; MAP three
                     kinase 1"
                     /codon_start=1
                     /product="mitogen-activated protein kinase kinase kinase 4
                     isoform a"
                     /protein_id="NP_005913.3"
                     /db_xref="CCDS:CCDS34565.1"
                     /db_xref="GeneID:4216"
                     /db_xref="HGNC:HGNC:6856"
                     /db_xref="MIM:602425"
                     /translation="MREAAAALVPPPAFAVTPAAAMEEPPPPPPPPPPPPEPETESEP
                     ECCLAARQEGTLGDSACKSPESDLEDFSDETNTENLYGTSPPSTPRQMKRMSTKHQRN
                     NVGRPASRSNLKEKMNAPNQPPHKDTGKTVENVEEYSYKQEKKIRAALRTTERDRKKN
                     VQCSFMLDSVGGSLPKKSIPDVDLNKPYLSLGCSNAKLPVSVPMPIARPARQTSRTDC
                     PADRLKFFETLRLLLKLTSVSKKKDREQRGQENTSGFWLNRSNELIWLELQAWHAGRT
                     INDQDFFLYTARQAIPDIINEILTFKVDYGSFAFVRDRAGFNGTSVEGQCKATPGTKI
                     VGYSTHHEHLQRQRVSFEQVKRIMELLEYIEALYPSLQALQKDYEKYAAKDFQDRVQA
                     LCLWLNITKDLNQKLRIMGTVLGIKNLSDIGWPVFEIPSPRPSKGNEPEYEGDDTEGE
                     LKELESSTDESEEEQISDPRVPEIRQPIDNSFDIQSRDCISKKLERLESEDDSLGWGA
                     PDWSTEAGFSRHCLTSIYRPFVDKALKQMGLRKLILRLHKLMDGSLQRARIALVKNDR
                     PVEFSEFPDPMWGSDYVQLSRTPPSSEEKCSAVSWEELKAMDLPSFEPAFLVLCRVLL
                     NVIHECLKLRLEQRPAGEPSLLSIKQLVRECKEVLKGGLLMKQYYQFMLQEVLEDLEK
                     PDCNIDAFEEDLHKMLMVYFDYMRSWIQMLQQLPQASHSLKNLLEEEWNFTKEITHYI
                     RGGEAQAGKLFCDIAGMLLKSTGSFLEFGLQESCAEFWTSADDSSASDEIRRSVIEIS
                     RALKELFHEARERASKALGFAKMLRKDLEIAAEFRLSAPVRDLLDVLKSKQYVKVQIP
                     GLENLQMFVPDTLAEEKSIILQLLNAAAGKDCSKDSDDVLIDAYLLLTKHGDRARDSE
                     DSWGTWEAQPVKVVPQVETVDTLRSMQVDNLLLVVMQSAHLTIQRKAFQQSIEGLMTL
                     CQEQTSSQPVIAKALQQLKNDALELCNRISNAIDRVDHMFTSEFDAEVDESESVTLQQ
                     YYREAMIQGYNFGFEYHKEVVRLMSGEFRQKIGDKYISFARKWMNYVLTKCESGRGTR
                     PRWATQGFDFLQAIEPAFISALPEDDFLSLQALMNECIGHVIGKPHSPVTGLYLAIHR
                     NSPRPMKVPRCHSDPPNPHLIIPTPEGFSTRSMPSDARSHGSPAAAAAAAAAAVAASR
                     PSPSGGDSVLPKSISSAHDTRGSSVPENDRLASIAAELQFRSLSRHSSPTEERDEPAY
                     PRGDSSGSTRRSWELRTLISQSKDTASKLGPIEAIQKSVRLFEEKRYREMRRKNIIGQ
                     VCDTPKSYDNVMHVGLRKVTFKWQRGNKIGEGQYGKVYTCISVDTGELMAMKEIRFQP
                     NDHKTIKETADELKIFEGIKHPNLVRYFGVELHREEMYIFMEYCDEGTLEEVSRLGLQ
                     EHVIRLYSKQITIAINVLHEHGIVHRDIKGANIFLTSSGLIKLGDFGCSVKLKNNAQT
                     MPGEVNSTLGTAAYMAPEVITRAKGEGHGRAADIWSLGCVVIEMVTGKRPWHEYEHNF
                     QIMYKVGMGHKPPIPERLSPEGKDFLSHCLESDPKMRWTASQLLDHSFVKVCTDEE"
     exon            316..506
                     /gene="MAP3K4"
                     /gene_synonym="MAPKKK4; MEKK 4; MEKK4; MTK1; PRO0412"
                     /inference="alignment:Splign:2.1.0"
     exon            507..1870
                     /gene="MAP3K4"
                     /gene_synonym="MAPKKK4; MEKK 4; MEKK4; MTK1; PRO0412"
                     /inference="alignment:Splign:2.1.0"
     exon            1871..2113
                     /gene="MAP3K4"
                     /gene_synonym="MAPKKK4; MEKK 4; MEKK4; MTK1; PRO0412"
                     /inference="alignment:Splign:2.1.0"
     exon            2114..2260
                     /gene="MAP3K4"
                     /gene_synonym="MAPKKK4; MEKK 4; MEKK4; MTK1; PRO0412"
                     /inference="alignment:Splign:2.1.0"
     exon            2261..2418
                     /gene="MAP3K4"
                     /gene_synonym="MAPKKK4; MEKK 4; MEKK4; MTK1; PRO0412"
                     /inference="alignment:Splign:2.1.0"
     exon            2419..2535
                     /gene="MAP3K4"
                     /gene_synonym="MAPKKK4; MEKK 4; MEKK4; MTK1; PRO0412"
                     /inference="alignment:Splign:2.1.0"
     exon            2536..2635
                     /gene="MAP3K4"
                     /gene_synonym="MAPKKK4; MEKK 4; MEKK4; MTK1; PRO0412"
                     /inference="alignment:Splign:2.1.0"
     exon            2636..2719
                     /gene="MAP3K4"
                     /gene_synonym="MAPKKK4; MEKK 4; MEKK4; MTK1; PRO0412"
                     /inference="alignment:Splign:2.1.0"
     exon            2720..2986
                     /gene="MAP3K4"
                     /gene_synonym="MAPKKK4; MEKK 4; MEKK4; MTK1; PRO0412"
                     /inference="alignment:Splign:2.1.0"
     exon            2987..3136
                     /gene="MAP3K4"
                     /gene_synonym="MAPKKK4; MEKK 4; MEKK4; MTK1; PRO0412"
                     /inference="alignment:Splign:2.1.0"
     exon            3137..3298
                     /gene="MAP3K4"
                     /gene_synonym="MAPKKK4; MEKK 4; MEKK4; MTK1; PRO0412"
                     /inference="alignment:Splign:2.1.0"
     exon            3299..3432
                     /gene="MAP3K4"
                     /gene_synonym="MAPKKK4; MEKK 4; MEKK4; MTK1; PRO0412"
                     /inference="alignment:Splign:2.1.0"
     exon            3433..3511
                     /gene="MAP3K4"
                     /gene_synonym="MAPKKK4; MEKK 4; MEKK4; MTK1; PRO0412"
                     /inference="alignment:Splign:2.1.0"
     exon            3512..3590
                     /gene="MAP3K4"
                     /gene_synonym="MAPKKK4; MEKK 4; MEKK4; MTK1; PRO0412"
                     /inference="alignment:Splign:2.1.0"
     exon            3591..3687
                     /gene="MAP3K4"
                     /gene_synonym="MAPKKK4; MEKK 4; MEKK4; MTK1; PRO0412"
                     /inference="alignment:Splign:2.1.0"
     exon            3688..3837
                     /gene="MAP3K4"
                     /gene_synonym="MAPKKK4; MEKK 4; MEKK4; MTK1; PRO0412"
                     /inference="alignment:Splign:2.1.0"
     exon            3838..3938
                     /gene="MAP3K4"
                     /gene_synonym="MAPKKK4; MEKK 4; MEKK4; MTK1; PRO0412"
                     /inference="alignment:Splign:2.1.0"
     exon            3939..4019
                     /gene="MAP3K4"
                     /gene_synonym="MAPKKK4; MEKK 4; MEKK4; MTK1; PRO0412"
                     /inference="alignment:Splign:2.1.0"
     exon            4020..4211
                     /gene="MAP3K4"
                     /gene_synonym="MAPKKK4; MEKK 4; MEKK4; MTK1; PRO0412"
                     /inference="alignment:Splign:2.1.0"
     exon            4212..4282
                     /gene="MAP3K4"
                     /gene_synonym="MAPKKK4; MEKK 4; MEKK4; MTK1; PRO0412"
                     /inference="alignment:Splign:2.1.0"
     exon            4283..4399
                     /gene="MAP3K4"
                     /gene_synonym="MAPKKK4; MEKK 4; MEKK4; MTK1; PRO0412"
                     /inference="alignment:Splign:2.1.0"
     exon            4400..4559
                     /gene="MAP3K4"
                     /gene_synonym="MAPKKK4; MEKK 4; MEKK4; MTK1; PRO0412"
                     /inference="alignment:Splign:2.1.0"
     exon            4560..4682
                     /gene="MAP3K4"
                     /gene_synonym="MAPKKK4; MEKK 4; MEKK4; MTK1; PRO0412"
                     /inference="alignment:Splign:2.1.0"
     exon            4683..4789
                     /gene="MAP3K4"
                     /gene_synonym="MAPKKK4; MEKK 4; MEKK4; MTK1; PRO0412"
                     /inference="alignment:Splign:2.1.0"
     exon            4790..4969
                     /gene="MAP3K4"
                     /gene_synonym="MAPKKK4; MEKK 4; MEKK4; MTK1; PRO0412"
                     /inference="alignment:Splign:2.1.0"
     exon            4970..5500
                     /gene="MAP3K4"
                     /gene_synonym="MAPKKK4; MEKK 4; MEKK4; MTK1; PRO0412"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5478..5483
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MAP3K4"
                     /gene_synonym="MAPKKK4; MEKK 4; MEKK4; MTK1; PRO0412"
                     /note="hexamer: AATAAA"
     polyA_site      5500
                     /gene="MAP3K4"
                     /gene_synonym="MAPKKK4; MEKK 4; MEKK4; MTK1; PRO0412"
                     /note="major polyA site"
ORIGIN      
        1 gctcggccgg tcgccgtttc caagatggcc gcggcgcgca cggctcctgc ggcggggtag
       61 aggcggaggc ggagtcgagt cactcccgca cttcggggct ccggtgcccc gcgccaggct
      121 gcagcttact gcccgccgcg gccatgcggg gctccgtgca cggatgagag aagccgctgc
      181 cgcgctggtc cctcctcccg cctttgccgt cacgcctgcc gccgccatgg aggagccgcc
      241 gccaccgccg ccgccgccac caccgccacc ggaacccgag accgagtcag aacccgagtg
      301 ctgcttggcg gcgaggcaag agggcacatt gggagattca gcttgcaaga gtcctgaatc
      361 tgatctagaa gacttctccg atgaaacaaa tacagagaat ctttatggta cctctccccc
      421 cagcacacct cgacagatga aacgcatgtc aaccaaacat cagaggaata atgtggggag
      481 gccagccagt cggtctaatt tgaaagaaaa aatgaatgca ccaaatcagc ctccacataa
      541 agacactgga aaaacagtgg agaatgtgga agaatacagc tataagcagg agaaaaagat
      601 ccgagcagct cttagaacaa cagagcgtga tcgtaaaaaa aatgtacagt gctcattcat
      661 gttagactca gtgggtggat ctttgccaaa aaaatcaatt ccagatgtgg atctcaataa
      721 gccttacctc agccttggct gtagcaatgc taagcttcca gtatctgtgc ccatgcctat
      781 agccagacct gcacgccaga cttctaggac tgactgtcca gcagatcgtt taaagttttt
      841 tgaaacttta cgacttttgc taaagcttac ctcagtctca aagaaaaaag acagggagca
      901 aagaggacaa gaaaatacgt ctggtttctg gcttaaccga tctaacgaac tgatctggtt
      961 agagctacaa gcctggcatg caggacggac aattaacgac caggacttct ttttatatac
     1021 agcccgtcaa gccatcccag atattattaa tgaaatcctt actttcaaag tcgactatgg
     1081 gagcttcgcc tttgttagag atagagctgg ttttaatggt acttcagtag aagggcagtg
     1141 caaagccact cctggaacaa agattgtagg ttactcaaca catcatgagc atctccaacg
     1201 ccagagggtc tcatttgagc aggtaaaacg gataatggag ctgctagagt acatagaagc
     1261 actttatcca tcattgcagg ctcttcagaa ggactatgaa aaatatgctg caaaagactt
     1321 ccaggacagg gtgcaggcac tctgtttgtg gttaaacatc acaaaagact taaatcagaa
     1381 attaaggatt atgggcactg ttttgggcat caagaattta tcagacattg gctggccagt
     1441 gtttgaaatc ccttcccctc gaccatccaa aggtaatgag ccggagtatg agggtgatga
     1501 cacagaagga gaattaaagg agttggaaag tagtacggat gagagtgaag aagaacaaat
     1561 ctctgatcct agggtaccgg aaatcagaca gcccatagat aacagcttcg acatccagtc
     1621 gcgggactgc atatccaaga agcttgagag gctcgaatct gaggatgatt ctcttggctg
     1681 gggagcacca gactggagca cagaagcagg ctttagtaga cattgtctga cttctattta
     1741 tagaccattt gtagacaaag cactgaagca gatggggtta agaaagttaa ttttaagact
     1801 tcacaagcta atggatggtt ccttgcaaag ggcacgtata gcattggtaa agaacgatcg
     1861 tccagtggag ttttctgaat ttccagatcc catgtggggt tcagattatg tgcagttgtc
     1921 aaggacacca ccttcatctg aggagaaatg cagtgctgtg tcgtgggagg agctgaaggc
     1981 catggattta ccttcattcg aacctgcctt cctagttctc tgccgagtcc ttctgaatgt
     2041 catacatgag tgtctgaagt taagattgga gcagagacct gctggagaac catctctctt
     2101 gagtattaag cagctggtga gagagtgtaa ggaggtcctg aagggcggcc tgctgatgaa
     2161 gcagtactac cagttcatgc tgcaggaggt tctggaggac ttggagaagc ccgactgcaa
     2221 cattgacgct tttgaagagg atctacataa aatgcttatg gtgtattttg attacatgag
     2281 aagctggatc caaatgctac agcaattacc tcaagcatcg catagtttaa aaaatctgtt
     2341 agaagaagaa tggaatttca ccaaagaaat aactcattac atacggggag gagaagcaca
     2401 ggccgggaag cttttctgtg acattgcagg aatgctgctg aaatctacag gaagtttttt
     2461 agaatttggc ttacaggaga gctgtgctga attttggact agtgcggatg acagcagtgc
     2521 ttccgacgaa atcaggaggt ctgttataga gatcagtcga gccctgaagg agctcttcca
     2581 tgaagccaga gaaagggctt ccaaagcact tggatttgct aaaatgttga gaaaggacct
     2641 ggaaatagca gcagaattca ggctttcagc cccagttaga gacctcctgg atgttctgaa
     2701 atcaaaacag tatgtcaagg tgcaaattcc tgggttagaa aacttgcaaa tgtttgttcc
     2761 agacactctt gctgaggaga agagtattat tttgcagtta ctcaatgcag ctgcaggaaa
     2821 ggactgttca aaagattcag atgacgtact catcgatgcc tatctgcttc tgaccaagca
     2881 cggtgatcga gcccgtgatt cagaggacag ctggggcacc tgggaggcac agcctgtcaa
     2941 agtcgtgcct caggtggaga ctgttgacac cctgagaagc atgcaggtgg ataatctttt
     3001 actagttgtc atgcagtctg cgcatctcac aattcagaga aaagctttcc agcagtccat
     3061 tgagggactt atgactctgt gccaggagca gacatccagt cagccggtca tcgccaaagc
     3121 tttgcagcag ctgaagaatg atgcattgga gctatgcaac aggataagca atgccattga
     3181 ccgcgtggac cacatgttca catcagaatt tgatgctgag gttgatgaat ctgaatctgt
     3241 caccttgcaa cagtactacc gagaagcaat gattcagggg tacaattttg gatttgagta
     3301 tcataaagaa gttgttcgtt tgatgtctgg ggagtttaga cagaagatag gagacaaata
     3361 tataagcttt gcccggaagt ggatgaatta tgtcctgact aaatgtgaga gtggtagagg
     3421 tacaagaccc aggtgggcga ctcaaggatt tgattttcta caagcaattg aacctgcctt
     3481 tatttcagct ttaccagaag atgacttctt gagtttacaa gccttgatga atgaatgcat
     3541 tggccatgtc ataggaaaac cacacagtcc tgttacaggt ttgtaccttg ccattcatcg
     3601 gaacagcccc cgtcctatga aggtacctcg atgccatagt gaccctccta acccacacct
     3661 cattatcccc actccagagg gattcagcac tcggagcatg ccttccgacg cgcggagcca
     3721 tggcagccct gctgctgctg ctgctgctgc tgctgctgct gttgctgcca gtcggcccag
     3781 cccctctggt ggtgactctg tgctgcccaa atccatcagc agtgcccatg ataccagggg
     3841 ttccagcgtt cctgaaaatg atcgattggc ttccatagct gctgaattgc agtttaggtc
     3901 cctgagtcgt cactcaagcc ccacggagga gcgagatgaa ccagcatatc caagaggaga
     3961 ttcaagtggg tccacaagaa gaagttggga acttcggaca ctaatcagcc agagtaaaga
     4021 tactgcttct aaactaggac ccatagaagc tatccagaag tcagtccgat tgtttgaaga
     4081 aaagaggtac cgagaaatga ggagaaagaa tatcattggt caagtttgtg atacgcctaa
     4141 gtcctatgat aatgttatgc acgttggctt gaggaaggtg accttcaaat ggcaaagagg
     4201 aaacaaaatt ggagaaggcc agtatgggaa ggtgtacacc tgcatcagcg tcgacaccgg
     4261 ggagctgatg gccatgaaag agattcgatt tcaacctaat gaccataaga ctatcaagga
     4321 aactgcagac gaattgaaaa tattcgaagg catcaaacac cccaatctgg ttcggtattt
     4381 tggtgtggag ctccatagag aagaaatgta catcttcatg gagtactgcg atgaggggac
     4441 tttagaagag gtgtcaaggc tgggacttca ggaacatgtg attaggctgt attcaaagca
     4501 gatcaccatt gcgatcaacg tcctccatga gcatggcata gtccaccgtg acattaaagg
     4561 tgccaatatc ttccttacct catctggatt aatcaaactg ggagattttg gatgttcagt
     4621 aaagctcaaa aacaatgccc agaccatgcc tggtgaagtg aacagcaccc tggggacagc
     4681 agcatacatg gcacctgaag tcatcactcg tgccaaagga gagggccatg ggcgtgcggc
     4741 cgacatctgg agtctggggt gtgttgtcat agagatggtg actggcaaga ggccttggca
     4801 tgagtatgag cacaactttc aaattatgta taaagtgggg atgggacata agccaccaat
     4861 ccctgaaaga ttaagccctg aaggaaagga cttcctttct cactgccttg agagtgaccc
     4921 aaagatgaga tggaccgcca gccagctcct cgaccattcg tttgtcaagg tttgcacaga
     4981 tgaagaatga agcctagtag aatatggact tggaaaattc tcttaatcac tactgtatgt
     5041 aatatttaca taaagactgt gctgagaagc agtataagcc tttttaacct tccaagactg
     5101 aagactgcac aggtgacaag cgtcacttct cctgctgctc ctgtttgtct gatgtggcaa
     5161 aaggccctct ggagggctgg tggccacgag gttaaagaag ctgcatgtta agtgccatta
     5221 ctactgtaca cggaccatcg cctctgtctc ctccgtgtct cgcgcgactg agaaccgtga
     5281 catcagcgta gtgttttgac ctttctaggt tcaaaagaag ttgtagtgtt atcaggcgtc
     5341 ccataccttg tttttaatct cctgtttgtt gagtgcactg actgtgaaac ctttaccttt
     5401 tttgttgttg ttggcaagct gcaggtttgt aatgcaaaag gctgattact gaaatttaag
     5461 aaaaaggttc ttttttcaat aaatggttta ttttaggaaa
//
