LOCUS       NM_016653               3859 bp    mRNA    linear   PRI 07-FEB-2025
DEFINITION  Homo sapiens mitogen-activated protein kinase kinase kinase 20
            (MAP3K20), transcript variant 1, mRNA.
ACCESSION   NM_016653
VERSION     NM_016653.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3859)
  AUTHORS   Brooks,D., Burke,E., Lee,S., Eble,T.N., O'Leary,M., Osei-Owusu,I.,
            Rehm,H.L., Dhar,S.U., Emrick,L., Bick,D., Nehrebecky,M.,
            Macnamara,E., Casas-Alba,D., Armstrong,J., Prat,C.,
            Martinez-Monseny,A.F., Palau,F., Liu,P., Adams,D., Lalani,S.,
            Rosenfeld,J.A. and Burrage,L.C.
  CONSRTM   Undiagnosed Diseases Network
  TITLE     Heterozygous MAP3K20 variants cause ectodermal dysplasia,
            craniosynostosis, sensorineural hearing loss, and limb anomalies
  JOURNAL   Hum Genet 143 (3), 279-291 (2024)
   PUBMED   38451290
  REMARK    GeneRIF: Heterozygous MAP3K20 variants cause ectodermal dysplasia,
            craniosynostosis, sensorineural hearing loss, and limb anomalies.
REFERENCE   2  (bases 1 to 3859)
  AUTHORS   Stonadge,A., Genzor,A.V., Russell,A., Hamed,M.F., Romero,N.,
            Evans,G., Pownall,M.E., Bekker-Jensen,S. and Blanco,G.
  TITLE     Myofibrillar myopathy hallmarks associated with ZAK deficiency
  JOURNAL   Hum Mol Genet 32 (17), 2751-2770 (2023)
   PUBMED   37427997
  REMARK    GeneRIF: Myofibrillar myopathy hallmarks associated with ZAK
            deficiency.
            Erratum:[Hum Mol Genet. 2023 Dec 1;32(24):3390. doi:
            10.1093/hmg/ddad168.. PMID: 37788121]
REFERENCE   3  (bases 1 to 3859)
  AUTHORS   Segal,D., Maier,S., Mastromarco,G.J., Qian,W.W., Nabeel-Shah,S.,
            Lee,H., Moore,G., Lacoste,J., Larsen,B., Lin,Z.Y.,
            Selvabaskaran,A., Liu,K., Smibert,C., Zhang,Z., Greenblatt,J.,
            Peng,J., Lee,H.O., Gingras,A.C. and Taipale,M.
  TITLE     A central chaperone-like role for 14-3-3 proteins in human cells
  JOURNAL   Mol Cell 83 (6), 974-993 (2023)
   PUBMED   36931259
REFERENCE   4  (bases 1 to 3859)
  AUTHORS   Shu,S., Liu,H., Yang,J., Tang,H., Li,H., Liu,Z., Zhou,M., Zhu,F.,
            Hu,Z., Ding,K., Lu,X. and Nie,J.
  TITLE     Targeted inhibition of ZAK ameliorates renal interstitial fibrosis
  JOURNAL   Transl Res 246, 49-65 (2022)
   PUBMED   35276386
  REMARK    GeneRIF: Targeted inhibition of ZAK ameliorates renal interstitial
            fibrosis.
REFERENCE   5  (bases 1 to 3859)
  AUTHORS   Robinson,K.S., Toh,G.A., Rozario,P., Chua,R., Bauernfried,S.,
            Sun,Z., Firdaus,M.J., Bayat,S., Nadkarni,R., Poh,Z.S., Tham,K.C.,
            Harapas,C.R., Lim,C.K., Chu,W., Tay,C.W.S., Tan,K.Y., Zhao,T.,
            Bonnard,C., Sobota,R., Connolly,J.E., Common,J., Masters,S.L.,
            Chen,K.W., Ho,L., Wu,B., Hornung,V. and Zhong,F.L.
  TITLE     ZAKalpha-driven ribotoxic stress response activates the human NLRP1
            inflammasome
  JOURNAL   Science 377 (6603), 328-335 (2022)
   PUBMED   35857590
  REMARK    GeneRIF: ZAKalpha-driven ribotoxic stress response activates the
            human NLRP1 inflammasome.
REFERENCE   6  (bases 1 to 3859)
  AUTHORS   Yang,J.J.
  TITLE     Mixed lineage kinase ZAK utilizing MKK7 and not MKK4 to activate
            the c-Jun N-terminal kinase and playing a role in the cell arrest
  JOURNAL   Biochem Biophys Res Commun 297 (1), 105-110 (2002)
   PUBMED   12220515
REFERENCE   7  (bases 1 to 3859)
  AUTHORS   Gross,E.A., Callow,M.G., Waldbaum,L., Thomas,S. and Ruggieri,R.
  TITLE     MRK, a mixed lineage kinase-related molecule that plays a role in
            gamma-radiation-induced cell cycle arrest
  JOURNAL   J Biol Chem 277 (16), 13873-13882 (2002)
   PUBMED   11836244
REFERENCE   8  (bases 1 to 3859)
  AUTHORS   Bloem,L.J., Pickard,T.R., Acton,S., Donoghue,M., Beavis,R.C.,
            Knierman,M.D. and Wang,X.
  TITLE     Tissue distribution and functional expression of a cDNA encoding a
            novel mixed lineage kinase
  JOURNAL   J Mol Cell Cardiol 33 (9), 1739-1750 (2001)
   PUBMED   11549352
REFERENCE   9  (bases 1 to 3859)
  AUTHORS   Gotoh,I., Adachi,M. and Nishida,E.
  TITLE     Identification and characterization of a novel MAP kinase kinase
            kinase, MLTK
  JOURNAL   J Biol Chem 276 (6), 4276-4286 (2001)
   PUBMED   11042189
REFERENCE   10 (bases 1 to 3859)
  AUTHORS   Liu,T.C., Huang,C.J., Chu,Y.C., Wei,C.C., Chou,C.C., Chou,M.Y.,
            Chou,C.K. and Yang,J.J.
  TITLE     Cloning and expression of ZAK, a mixed lineage kinase-like protein
            containing a leucine-zipper and a sterile-alpha motif
  JOURNAL   Biochem Biophys Res Commun 274 (3), 811-816 (2000)
   PUBMED   10924358
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC019046.4, AC013461.9 and
            AC092573.2.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_016653.2.
            
            Summary: This gene is a member of the MAPKKK family of signal
            transduction molecules and encodes a protein with an N-terminal
            kinase catalytic domain, followed by a leucine zipper motif and a
            sterile-alpha motif (SAM). This magnesium-binding protein forms
            homodimers and is located in the cytoplasm. The protein mediates
            gamma radiation signaling leading to cell cycle arrest and activity
            of this protein plays a role in cell cycle checkpoint regulation in
            cells. The protein also has pro-apoptotic activity. Alternate
            transcriptional splice variants, encoding different isoforms, have
            been characterized. [provided by RefSeq, Jul 2008].
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1, also referred to as
            MRK-alpha and MLTK-alpha).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF480461.1, AF251441.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000375213.8/ ENSP00000364361.3
            RefSeq Select criteria :: based on conservation, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-157               AC019046.4         75681-75837         c
            158-350             AC019046.4         60493-60685         c
            351-438             AC013461.9         20123-20210
            439-540             AC013461.9         33172-33273
            541-606             AC013461.9         37876-37941
            607-635             AC013461.9         41213-41241
            636-773             AC013461.9         41358-41495
            774-860             AC013461.9         48344-48430
            861-935             AC013461.9         54114-54188
            936-1042            AC013461.9         60047-60153
            1043-1178           AC013461.9         67433-67568
            1179-1223           AC013461.9         80007-80051
            1224-1254           AC013461.9         82510-82540
            1255-1394           AC013461.9         82638-82777
            1395-1457           AC013461.9         88691-88753
            1458-1550           AC013461.9         89722-89814
            1551-1667           AC092573.2         3880-3996
            1668-1742           AC092573.2         6244-6318
            1743-1893           AC092573.2         8926-9076
            1894-3859           AC092573.2         11231-13196
FEATURES             Location/Qualifiers
     source          1..3859
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="2"
                     /map="2q31.1"
     gene            1..3859
                     /gene="MAP3K20"
                     /gene_synonym="AZK; CNM6; MLK7; mlklak; MLT; MLTK;
                     MLTKalpha; MLTKbeta; MRK; pk; SFMMP; ZAK"
                     /note="mitogen-activated protein kinase kinase kinase 20"
                     /db_xref="GeneID:51776"
                     /db_xref="HGNC:HGNC:17797"
                     /db_xref="MIM:609479"
     exon            1..157
                     /gene="MAP3K20"
                     /gene_synonym="AZK; CNM6; MLK7; mlklak; MLT; MLTK;
                     MLTKalpha; MLTKbeta; MRK; pk; SFMMP; ZAK"
                     /inference="alignment:Splign:2.1.0"
     exon            158..350
                     /gene="MAP3K20"
                     /gene_synonym="AZK; CNM6; MLK7; mlklak; MLT; MLTK;
                     MLTKalpha; MLTKbeta; MRK; pk; SFMMP; ZAK"
                     /inference="alignment:Splign:2.1.0"
     CDS             192..2594
                     /gene="MAP3K20"
                     /gene_synonym="AZK; CNM6; MLK7; mlklak; MLT; MLTK;
                     MLTKalpha; MLTKbeta; MRK; pk; SFMMP; ZAK"
                     /EC_number="2.7.11.25"
                     /note="isoform 1 is encoded by transcript variant 1; mixed
                     lineage kinase-related kinase MRK-beta; MLK-like
                     mitogen-activated protein triple kinase; mixed lineage
                     kinase 7; mitogen-activated protein kinase kinase kinase
                     MLT; mixed lineage kinase with a leucine zipper and a
                     sterile alpha motif; HCCS-4; human cervical cancer
                     suppressor gene 4 protein; sterile alpha motif and leucine
                     zipper containing kinase AZK; ZAK1 homolog, leucine zipper
                     and sterile-alpha motif kinase"
                     /codon_start=1
                     /product="mitogen-activated protein kinase kinase kinase
                     20 isoform 1"
                     /protein_id="NP_057737.2"
                     /db_xref="CCDS:CCDS42777.1"
                     /db_xref="GeneID:51776"
                     /db_xref="HGNC:HGNC:17797"
                     /db_xref="MIM:609479"
                     /translation="MSSLGASFVQIKFDDLQFFENCGGGSFGSVYRAKWISQDKEVAV
                     KKLLKIEKEAEILSVLSHRNIIQFYGVILEPPNYGIVTEYASLGSLYDYINSNRSEEM
                     DMDHIMTWATDVAKGMHYLHMEAPVKVIHRDLKSRNVVIAADGVLKICDFGASRFHNH
                     TTHMSLVGTFPWMAPEVIQSLPVSETCDTYSYGVVLWEMLTREVPFKGLEGLQVAWLV
                     VEKNERLTIPSSCPRSFAELLHQCWEADAKKRPSFKQIISILESMSNDTSLPDKCNSF
                     LHNKAEWRCEIEATLERLKKLERDLSFKEQELKERERRLKMWEQKLTEQSNTPLLPSF
                     EIGAWTEDDVYCWVQQLVRKGDSSAEMSVYASLFKENNITGKRLLLLEEEDLKDMGIV
                     SKGHIIHFKSAIEKLTHDYINLFHFPPLIKDSGGEPEENEEKIVNLELVFGFHLKPGT
                     GPQDCKWKMYMEMDGDEIAITYIKDVTFNTNLPDAEILKMTKPPFVMEKWIVGIAKSQ
                     TVECTVTYESDVRTPKSTKHVHSIQWSRTKPQDEVKAVQLAIQTLFTNSDGNPGSRSD
                     SSADCQWLDTLRMRQIASNTSLQRSQSNPILGSPFFSHFDGQDSYAAAVRRPQVPIKY
                     QQITPVNQSRSSSPTQYGLTKNFSSLHLNSRDSGFSSGNTDTSSERGRYSDRSRNKYG
                     RGSISLNSSPRGRYSGKSQHSTPSRGRYPGKFYRVSQSALNPHQSPDFKRSPRDLHQP
                     NTIPGMPLHPETDSRASEEDSKVSEGGWTKVEYRKKPHRPSPAKTNKERARGDHRGWR
                     NF"
     exon            351..438
                     /gene="MAP3K20"
                     /gene_synonym="AZK; CNM6; MLK7; mlklak; MLT; MLTK;
                     MLTKalpha; MLTKbeta; MRK; pk; SFMMP; ZAK"
                     /inference="alignment:Splign:2.1.0"
     exon            439..540
                     /gene="MAP3K20"
                     /gene_synonym="AZK; CNM6; MLK7; mlklak; MLT; MLTK;
                     MLTKalpha; MLTKbeta; MRK; pk; SFMMP; ZAK"
                     /inference="alignment:Splign:2.1.0"
     exon            541..606
                     /gene="MAP3K20"
                     /gene_synonym="AZK; CNM6; MLK7; mlklak; MLT; MLTK;
                     MLTKalpha; MLTKbeta; MRK; pk; SFMMP; ZAK"
                     /inference="alignment:Splign:2.1.0"
     exon            607..635
                     /gene="MAP3K20"
                     /gene_synonym="AZK; CNM6; MLK7; mlklak; MLT; MLTK;
                     MLTKalpha; MLTKbeta; MRK; pk; SFMMP; ZAK"
                     /inference="alignment:Splign:2.1.0"
     exon            636..773
                     /gene="MAP3K20"
                     /gene_synonym="AZK; CNM6; MLK7; mlklak; MLT; MLTK;
                     MLTKalpha; MLTKbeta; MRK; pk; SFMMP; ZAK"
                     /inference="alignment:Splign:2.1.0"
     exon            774..860
                     /gene="MAP3K20"
                     /gene_synonym="AZK; CNM6; MLK7; mlklak; MLT; MLTK;
                     MLTKalpha; MLTKbeta; MRK; pk; SFMMP; ZAK"
                     /inference="alignment:Splign:2.1.0"
     exon            861..935
                     /gene="MAP3K20"
                     /gene_synonym="AZK; CNM6; MLK7; mlklak; MLT; MLTK;
                     MLTKalpha; MLTKbeta; MRK; pk; SFMMP; ZAK"
                     /inference="alignment:Splign:2.1.0"
     exon            936..1042
                     /gene="MAP3K20"
                     /gene_synonym="AZK; CNM6; MLK7; mlklak; MLT; MLTK;
                     MLTKalpha; MLTKbeta; MRK; pk; SFMMP; ZAK"
                     /inference="alignment:Splign:2.1.0"
     exon            1043..1178
                     /gene="MAP3K20"
                     /gene_synonym="AZK; CNM6; MLK7; mlklak; MLT; MLTK;
                     MLTKalpha; MLTKbeta; MRK; pk; SFMMP; ZAK"
                     /inference="alignment:Splign:2.1.0"
     exon            1179..1223
                     /gene="MAP3K20"
                     /gene_synonym="AZK; CNM6; MLK7; mlklak; MLT; MLTK;
                     MLTKalpha; MLTKbeta; MRK; pk; SFMMP; ZAK"
                     /inference="alignment:Splign:2.1.0"
     exon            1224..1254
                     /gene="MAP3K20"
                     /gene_synonym="AZK; CNM6; MLK7; mlklak; MLT; MLTK;
                     MLTKalpha; MLTKbeta; MRK; pk; SFMMP; ZAK"
                     /inference="alignment:Splign:2.1.0"
     exon            1255..1394
                     /gene="MAP3K20"
                     /gene_synonym="AZK; CNM6; MLK7; mlklak; MLT; MLTK;
                     MLTKalpha; MLTKbeta; MRK; pk; SFMMP; ZAK"
                     /inference="alignment:Splign:2.1.0"
     exon            1395..1457
                     /gene="MAP3K20"
                     /gene_synonym="AZK; CNM6; MLK7; mlklak; MLT; MLTK;
                     MLTKalpha; MLTKbeta; MRK; pk; SFMMP; ZAK"
                     /inference="alignment:Splign:2.1.0"
     exon            1458..1550
                     /gene="MAP3K20"
                     /gene_synonym="AZK; CNM6; MLK7; mlklak; MLT; MLTK;
                     MLTKalpha; MLTKbeta; MRK; pk; SFMMP; ZAK"
                     /inference="alignment:Splign:2.1.0"
     exon            1551..1667
                     /gene="MAP3K20"
                     /gene_synonym="AZK; CNM6; MLK7; mlklak; MLT; MLTK;
                     MLTKalpha; MLTKbeta; MRK; pk; SFMMP; ZAK"
                     /inference="alignment:Splign:2.1.0"
     exon            1668..1742
                     /gene="MAP3K20"
                     /gene_synonym="AZK; CNM6; MLK7; mlklak; MLT; MLTK;
                     MLTKalpha; MLTKbeta; MRK; pk; SFMMP; ZAK"
                     /inference="alignment:Splign:2.1.0"
     exon            1743..1893
                     /gene="MAP3K20"
                     /gene_synonym="AZK; CNM6; MLK7; mlklak; MLT; MLTK;
                     MLTKalpha; MLTKbeta; MRK; pk; SFMMP; ZAK"
                     /inference="alignment:Splign:2.1.0"
     exon            1894..3859
                     /gene="MAP3K20"
                     /gene_synonym="AZK; CNM6; MLK7; mlklak; MLT; MLTK;
                     MLTKalpha; MLTKbeta; MRK; pk; SFMMP; ZAK"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3341..3346
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MAP3K20"
                     /gene_synonym="AZK; CNM6; MLK7; mlklak; MLT; MLTK;
                     MLTKalpha; MLTKbeta; MRK; pk; SFMMP; ZAK"
                     /note="hexamer: AATAAA"
     polyA_site      3371
                     /gene="MAP3K20"
                     /gene_synonym="AZK; CNM6; MLK7; mlklak; MLT; MLTK;
                     MLTKalpha; MLTKbeta; MRK; pk; SFMMP; ZAK"
                     /note="major polyA site"
     regulatory      3834..3839
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MAP3K20"
                     /gene_synonym="AZK; CNM6; MLK7; mlklak; MLT; MLTK;
                     MLTKalpha; MLTKbeta; MRK; pk; SFMMP; ZAK"
                     /note="hexamer: AATAAA"
     polyA_site      3859
                     /gene="MAP3K20"
                     /gene_synonym="AZK; CNM6; MLK7; mlklak; MLT; MLTK;
                     MLTKalpha; MLTKbeta; MRK; pk; SFMMP; ZAK"
ORIGIN      
        1 ctcattggcg ccgtgcagag aggcggaatg ttcaactcct aactgcagcg gaaacgtggg
       61 agccgcgcgg gccgctgtcg tcccaacccc cgccgccctc gtcgcgcgcg gggcctccgc
      121 gcccccggct gctgctcacg ccccgcccgg gagccagatt ttgtggaagt ataatacttt
      181 gtcattatga gatgtcgtct ctcggtgcct cctttgtgca aattaaattt gatgacttgc
      241 agttttttga aaactgcggt ggaggaagtt ttgggagtgt ttatcgagcc aaatggatat
      301 cacaggacaa ggaggtggct gtaaagaagc tcctcaaaat agagaaagag gcagaaatac
      361 tcagtgtcct cagtcacaga aacatcatcc agttttatgg agtaattctt gaacctccca
      421 actatggcat tgtcacagaa tatgcttctc tgggatcact ctatgattac attaacagta
      481 acagaagtga ggagatggat atggatcaca ttatgacctg ggccactgat gtagccaaag
      541 gaatgcatta tttacatatg gaggctcctg tcaaggtgat tcacagagac ctcaagtcaa
      601 gaaacgttgt tatagctgct gatggagtat tgaagatctg tgactttggt gcctctcggt
      661 tccataacca tacaacacac atgtccttgg ttggaacttt cccatggatg gctccagaag
      721 ttatccagag tctccctgtg tcagaaactt gtgacacata ttcctatggt gtggttctct
      781 gggagatgct aacaagggag gtccccttta aaggtttgga aggattacaa gtagcttggc
      841 ttgtagtgga aaaaaacgag agattaacca ttccaagcag ttgccccaga agttttgctg
      901 aactgttaca tcagtgttgg gaagctgatg ccaagaaacg gccatcattc aagcaaatca
      961 tttcaatcct ggagtccatg tcaaatgaca cgagccttcc tgacaagtgt aactcattcc
     1021 tacacaacaa ggcggagtgg aggtgcgaaa ttgaggcaac tcttgagagg ctaaagaaac
     1081 tagagcgtga tctcagcttt aaggagcagg agcttaaaga acgagaaaga cgtttaaaga
     1141 tgtgggagca aaagctgaca gagcagtcca acaccccgct gctgccttcc tttgagattg
     1201 gtgcatggac ggaagacgat gtgtattgtt gggttcagca gctcgtcaga aaaggtgact
     1261 cttcagcaga gatgagtgta tatgcaagct tgtttaaaga aaacaacatt acagggaagc
     1321 ggctgctgct gctggaggaa gaagacctga aagacatggg cattgtctcc aaggggcata
     1381 tcattcactt caagtcagcc attgagaaat taacccatga ttacataaat ttgtttcact
     1441 tcccaccact aattaaggac tcaggaggtg aacctgaaga aaatgaggaa aaaatagtga
     1501 acctggaact ggtttttggt tttcacttga aaccaggaac tggcccacag gattgtaagt
     1561 ggaaaatgta tatggagatg gatggggatg aaattgcaat aacctacata aaagatgtga
     1621 cattcaacac taacctacct gatgcggaga ttttaaagat gacaaagcca ccatttgtaa
     1681 tggagaagtg gattgtagga atagcaaaaa gtcagactgt ggagtgcact gtcacatatg
     1741 agagtgatgt tagaactcca aaaagcacta aacatgtcca ttcgattcag tggagtagaa
     1801 caaaacctca ggatgaagtg aaagcagtcc aacttgccat tcagacatta ttcaccaatt
     1861 cagatggcaa ccctggaagc aggtccgact caagtgctga ttgccagtgg ttagatactc
     1921 tgaggatgcg gcagattgca tccaacactt ctttacagcg ttcccagagc aatcctattc
     1981 tggggtcacc gttcttctca cactttgatg gccaggattc ctacgctgct gctgtgagac
     2041 ggccccaggt gcccattaag tatcaacaga ttacacctgt gaaccagtcc agaagctcgt
     2101 ctcctactca gtatggactg accaaaaact tctcttccct acatctcaac tctagggaca
     2161 gtggcttttc cagtggcaat actgacacct cttcagagag gggtcgatac tcagacagaa
     2221 gcaggaacaa atatggacgt ggtagtatat cactcaattc ttctcctaga ggaagataca
     2281 gtggaaagag tcagcattcc actccttcaa gaggaagata ccctggaaag ttctacaggg
     2341 tttctcagtc agcactcaat cctcaccagt cgcctgactt caagagaagc cccagggacc
     2401 tccaccaacc caacaccata ccagggatgc ctttgcaccc tgagactgac tcaagagcca
     2461 gtgaagagga cagcaaagtc agcgaagggg gctggacaaa agtggaatac cggaaaaagc
     2521 cccacaggcc atctcccgcc aaaaccaata aagagagagc cagaggggac caccgtggat
     2581 ggagaaactt ttgatgaatt gaactacata gcttttctaa gcaggttaaa aaaaaaaaaa
     2641 aaaagaaatg taatggtttt tgataatatg atcccttcag attgaattaa cgaaaagaca
     2701 acacttccag tttttggatt gggaaatacc ttctaattga gactatagcc aaaccagggc
     2761 caaaattatg gatattggtc acccagtgat cataactagg cttgaaaatc actacacata
     2821 ttttctgcct tgagtgaaca tttttagagg aaaggttatg ccatcttttt accctaacca
     2881 ctgatattct ggttagcagg gccaggacaa ggggaaggaa aatgaggtca acaaaaaaat
     2941 caaattttta ggaaaagata agatgaatgt tactgatttt tccttttggc tgaggctgca
     3001 atatggcctg gcaaggcact gttactgatc ttgtctttaa cattttgata ttttgttcat
     3061 cataattttt gcatttattt ttttaaatat tgcattaaaa tatcatttag cttgattatc
     3121 gagttttttg gtttgaggtt ttttgttgct tcttttttct tttctttctt tccccctctt
     3181 ttttttggat gtccccttaa attttgtgcc caaggcaggt acctcactca tctcatcctt
     3241 ggctcagccc tgctggttag tatttagtat ttattttagt aagatatttg tgtctgtatg
     3301 atggtcagag ttgaactgat ctggcttgtc atttttcagt aataaaaaaa gttactgaat
     3361 ttaatgttga atatgatgca tatctcattc attacgattt atcagaaacc aaagatttaa
     3421 attgcctaga tttgtggttc tttctcttcc taagttccca gcgactgctt tcaaatacta
     3481 ttttctaaat ttcaccaaag gagcaaagag gataaaacaa cactccataa aggcctcttg
     3541 ggatgtcaga aatctaaaat ctaaaagaaa acagacacag agcaagacaa taacatcaca
     3601 agctaaaagc cagagaaatt taaaattacc aacatccttg ttggagtaag acagtaaata
     3661 tcagccttgc agcaagacag ctctgagcag ctgtgggcaa agaggtaaac cagtgggggt
     3721 gcaaggagac tgtctgcagc ttagggcaga aatggtggga tccaacttgt gaaatgcttc
     3781 atgttttaca aaccaaaaag tcaggtagca acaaacttat tgtatgtcaa atcaataaat
     3841 gttactttca atattctga
//
