LOCUS       NM_001371910           11360 bp    mRNA    linear   PRI 07-APR-2024
DEFINITION  Homo sapiens mitogen-activated protein kinase kinase kinase 2
            (MAP3K2), transcript variant 2, mRNA.
ACCESSION   NM_001371910 XM_024452552
VERSION     NM_001371910.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 11360)
  AUTHORS   Segal,D., Maier,S., Mastromarco,G.J., Qian,W.W., Nabeel-Shah,S.,
            Lee,H., Moore,G., Lacoste,J., Larsen,B., Lin,Z.Y.,
            Selvabaskaran,A., Liu,K., Smibert,C., Zhang,Z., Greenblatt,J.,
            Peng,J., Lee,H.O., Gingras,A.C. and Taipale,M.
  TITLE     A central chaperone-like role for 14-3-3 proteins in human cells
  JOURNAL   Mol Cell 83 (6), 974-993 (2023)
   PUBMED   36931259
REFERENCE   2  (bases 1 to 11360)
  AUTHORS   Huang,L., Guo,B., Yan,J., Wei,H., Liu,S. and Li,Y.
  TITLE     CircHSPG2 knockdown attenuates hypoxia-induced apoptosis,
            inflammation, and oxidative stress in human AC16 cardiomyocytes by
            regulating the miR-1184/MAP3K2 axis
  JOURNAL   Cell Stress Chaperones 28 (2), 177-190 (2023)
   PUBMED   36810972
  REMARK    GeneRIF: CircHSPG2 knockdown attenuates hypoxia-induced apoptosis,
            inflammation, and oxidative stress in human AC16 cardiomyocytes by
            regulating the miR-1184/MAP3K2 axis.
REFERENCE   3  (bases 1 to 11360)
  AUTHORS   Xu,W., Qian,L., Yuan,X. and Lu,Y.
  TITLE     Regulation of a Novel CircTRRAP/miR-761/MAP3K2 CeRNA Cascade in
            Inflammation, Apoptosis, and Oxidative Stress in Human AC16
            Cardiomyocytes under Hypoxia Conditions
  JOURNAL   Int Heart J 64 (3), 442-452 (2023)
   PUBMED   37258120
  REMARK    GeneRIF: Regulation of a Novel CircTRRAP/miR-761/MAP3K2 CeRNA
            Cascade in Inflammation, Apoptosis, and Oxidative Stress in Human
            AC16 Cardiomyocytes under Hypoxia Conditions.
REFERENCE   4  (bases 1 to 11360)
  AUTHORS   Du,N., Li,K., Wang,Y., Song,B., Zhou,X. and Duan,S.
  TITLE     CircRNA circBACH1 facilitates hepatitis B virus replication and
            hepatoma development by regulating the miR-200a-3p/MAP3K2 axis
  JOURNAL   Histol Histopathol 37 (9), 863-877 (2022)
   PUBMED   35352818
  REMARK    GeneRIF: CircRNA circBACH1 facilitates hepatitis B virus
            replication and hepatoma development by regulating the
            miR-200a-3p/MAP3K2 axis.
REFERENCE   5  (bases 1 to 11360)
  AUTHORS   Zhang,B., Wang,D., Wang,Y. and Chen,G.
  TITLE     miRNA-338-3p inhibits the migration, invasion and proliferation of
            human lung adenocarcinoma cells by targeting MAP3K2
  JOURNAL   Aging (Albany NY) 14 (15), 6094-6110 (2022)
   PUBMED   35929837
  REMARK    GeneRIF: miRNA-338-3p inhibits the migration, invasion and
            proliferation of human lung adenocarcinoma cells by targeting
            MAP3K2.
REFERENCE   6  (bases 1 to 11360)
  AUTHORS   Sun,W., Vincent,S., Settleman,J. and Johnson,G.L.
  TITLE     MEK kinase 2 binds and activates protein kinase C-related kinase 2.
            Bifurcation of kinase regulatory pathways at the level of an MAPK
            kinase kinase
  JOURNAL   J Biol Chem 275 (32), 24421-24428 (2000)
   PUBMED   10818102
REFERENCE   7  (bases 1 to 11360)
  AUTHORS   Cheng,J., Yang,J., Xia,Y., Karin,M. and Su,B.
  TITLE     Synergistic interaction of MEK kinase 2, c-Jun N-terminal kinase
            (JNK) kinase 2, and JNK1 results in efficient and specific JNK1
            activation
  JOURNAL   Mol Cell Biol 20 (7), 2334-2342 (2000)
   PUBMED   10713157
REFERENCE   8  (bases 1 to 11360)
  AUTHORS   Zhao,Q. and Lee,F.S.
  TITLE     Mitogen-activated protein kinase/ERK kinase kinases 2 and 3
            activate nuclear factor-kappaB through IkappaB kinase-alpha and
            IkappaB kinase-beta
  JOURNAL   J Biol Chem 274 (13), 8355-8358 (1999)
   PUBMED   10085062
REFERENCE   9  (bases 1 to 11360)
  AUTHORS   Fanger,G.R., Widmann,C., Porter,A.C., Sather,S., Johnson,G.L. and
            Vaillancourt,R.R.
  TITLE     14-3-3 proteins interact with specific MEK kinases
  JOURNAL   J Biol Chem 273 (6), 3476-3483 (1998)
   PUBMED   9452471
REFERENCE   10 (bases 1 to 11360)
  AUTHORS   Blank,J.L., Gerwins,P., Elliott,E.M., Sather,S. and Johnson,G.L.
  TITLE     Molecular cloning of mitogen-activated protein/ERK kinase kinases
            (MEKK) 2 and 3. Regulation of sequential phosphorylation pathways
            involving mitogen-activated protein kinase and c-Jun kinase
  JOURNAL   J Biol Chem 271 (10), 5361-5368 (1996)
   PUBMED   8621389
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC068282.8 and AC110926.5.
            
            On Aug 28, 2019 this sequence version replaced NM_001371910.1.
            
            Summary: The protein encoded by this gene is a member of
            serine/threonine protein kinase family. This kinase preferentially
            activates other kinases involved in the MAP kinase signaling
            pathway. This kinase has been shown to directly phosphorylate and
            activate Ikappa B kinases, and thus plays a role in NF-kappa B
            signaling pathway. This kinase has also been found to bind and
            activate protein kinase C-related kinase 2, which suggests its
            involvement in a regulated signaling process. [provided by RefSeq,
            Jul 2008].
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR11853567.17334.1,
                                           SRR11853564.16021.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000682094.1/ ENSP00000507315.1
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-524               AC068282.8         68542-69065         c
            525-593             AC068282.8         24216-24284         c
            594-712             AC068282.8         20022-20140         c
            713-753             AC068282.8         18828-18868         c
            754-853             AC068282.8         16960-17059         c
            854-967             AC068282.8         11482-11595         c
            968-1055            AC068282.8         11011-11098         c
            1056-1186           AC068282.8         7777-7907           c
            1187-1266           AC068282.8         6818-6897           c
            1267-1334           AC068282.8         5264-5331           c
            1335-1427           AC068282.8         4992-5084           c
            1428-1634           AC068282.8         3136-3342           c
            1635-1783           AC110926.5         190910-191058       c
            1784-1915           AC110926.5         190370-190501       c
            1916-2045           AC110926.5         187495-187624       c
            2046-2223           AC110926.5         181326-181503       c
            2224-11360          AC110926.5         171409-180545       c
FEATURES             Location/Qualifiers
     source          1..11360
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="2"
                     /map="2q14.3"
     gene            1..11360
                     /gene="MAP3K2"
                     /gene_synonym="MEKK2; MEKK2B"
                     /note="mitogen-activated protein kinase kinase kinase 2"
                     /db_xref="GeneID:10746"
                     /db_xref="HGNC:HGNC:6854"
                     /db_xref="MIM:609487"
     exon            1..524
                     /gene="MAP3K2"
                     /gene_synonym="MEKK2; MEKK2B"
                     /inference="alignment:Splign:2.1.0"
     exon            525..593
                     /gene="MAP3K2"
                     /gene_synonym="MEKK2; MEKK2B"
                     /inference="alignment:Splign:2.1.0"
     CDS             590..2449
                     /gene="MAP3K2"
                     /gene_synonym="MEKK2; MEKK2B"
                     /EC_number="2.7.11.25"
                     /note="MAP/ERK kinase kinase 2; MAPK/ERK kinase kinase 2;
                     MEK kinase 2; MEKK 2"
                     /codon_start=1
                     /product="mitogen-activated protein kinase kinase kinase
                     2"
                     /protein_id="NP_001358839.1"
                     /db_xref="CCDS:CCDS46404.1"
                     /db_xref="GeneID:10746"
                     /db_xref="HGNC:HGNC:6854"
                     /db_xref="MIM:609487"
                     /translation="MDDQQALNSIMQDLAVLHKASRPALSLQETRKAKSSSPKKQNDV
                     RVKFEHRGEKRILQFPRPVKLEDLRSKAKIAFGQSMDLHYTNNELVIPLTTQDDLDKA
                     VELLDRSIHMKSLKILLVINGSTQATNLEPLPSLEDLDNTVFGAERKKRLSIIGPTSR
                     DRSSPPPGYIPDELHQVARNGSFTSINSEGEFIPESMDQMLDPLSLSSPENSGSGSCP
                     SLDSPLDGESYPKSRMPRAQSYPDNHQEFSDYDNPIFEKFGKGGTYPRRYHVSYHHQE
                     YNDGRKTFPRARRTQGTSLRSPVSFSPTDHSLSTSSGSSIFTPEYDDSRIRRRGSDID
                     NPTLTVMDISPPSRSPRAPTNWRLGKLLGQGAFGRVYLCYDVDTGRELAVKQVQFDPD
                     SPETSKEVNALECEIQLLKNLLHERIVQYYGCLRDPQEKTLSIFMEYMPGGSIKDQLK
                     AYGALTENVTRKYTRQILEGVHYLHSNMIVHRDIKGANILRDSTGNVKLGDFGASKRL
                     QTICLSGTGMKSVTGTPYWMSPEVISGEGYGRKADIWSVACTVVEMLTEKPPWAEFEA
                     MAAIFKIATQPTNPKLPPHVSDYTRDFLKRIFVEAKLRPSADELLRHMFVHYH"
     exon            594..712
                     /gene="MAP3K2"
                     /gene_synonym="MEKK2; MEKK2B"
                     /inference="alignment:Splign:2.1.0"
     exon            713..753
                     /gene="MAP3K2"
                     /gene_synonym="MEKK2; MEKK2B"
                     /inference="alignment:Splign:2.1.0"
     exon            754..853
                     /gene="MAP3K2"
                     /gene_synonym="MEKK2; MEKK2B"
                     /inference="alignment:Splign:2.1.0"
     exon            854..967
                     /gene="MAP3K2"
                     /gene_synonym="MEKK2; MEKK2B"
                     /inference="alignment:Splign:2.1.0"
     exon            968..1055
                     /gene="MAP3K2"
                     /gene_synonym="MEKK2; MEKK2B"
                     /inference="alignment:Splign:2.1.0"
     exon            1056..1186
                     /gene="MAP3K2"
                     /gene_synonym="MEKK2; MEKK2B"
                     /inference="alignment:Splign:2.1.0"
     exon            1187..1266
                     /gene="MAP3K2"
                     /gene_synonym="MEKK2; MEKK2B"
                     /inference="alignment:Splign:2.1.0"
     exon            1267..1334
                     /gene="MAP3K2"
                     /gene_synonym="MEKK2; MEKK2B"
                     /inference="alignment:Splign:2.1.0"
     exon            1335..1427
                     /gene="MAP3K2"
                     /gene_synonym="MEKK2; MEKK2B"
                     /inference="alignment:Splign:2.1.0"
     exon            1428..1634
                     /gene="MAP3K2"
                     /gene_synonym="MEKK2; MEKK2B"
                     /inference="alignment:Splign:2.1.0"
     exon            1635..1783
                     /gene="MAP3K2"
                     /gene_synonym="MEKK2; MEKK2B"
                     /inference="alignment:Splign:2.1.0"
     exon            1784..1915
                     /gene="MAP3K2"
                     /gene_synonym="MEKK2; MEKK2B"
                     /inference="alignment:Splign:2.1.0"
     exon            1916..2045
                     /gene="MAP3K2"
                     /gene_synonym="MEKK2; MEKK2B"
                     /inference="alignment:Splign:2.1.0"
     exon            2046..2223
                     /gene="MAP3K2"
                     /gene_synonym="MEKK2; MEKK2B"
                     /inference="alignment:Splign:2.1.0"
     exon            2224..11360
                     /gene="MAP3K2"
                     /gene_synonym="MEKK2; MEKK2B"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5670..5675
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MAP3K2"
                     /gene_synonym="MEKK2; MEKK2B"
                     /note="hexamer: AATAAA"
     polyA_site      5688
                     /gene="MAP3K2"
                     /gene_synonym="MEKK2; MEKK2B"
                     /note="major polyA site"
     regulatory      9615..9620
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MAP3K2"
                     /gene_synonym="MEKK2; MEKK2B"
                     /note="hexamer: ATTAAA"
     polyA_site      9638
                     /gene="MAP3K2"
                     /gene_synonym="MEKK2; MEKK2B"
     regulatory      11332..11337
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MAP3K2"
                     /gene_synonym="MEKK2; MEKK2B"
                     /note="hexamer: AATAAA"
     polyA_site      11360
                     /gene="MAP3K2"
                     /gene_synonym="MEKK2; MEKK2B"
                     /note="major polyA site"
ORIGIN      
        1 gtcacgggtc cgtgtgtagt ggctgccggg ctgcctgccc tcagcggcgg cgcctgacga
       61 gggtgccgcg gccgctgggc gcagcgttcg ggggttgcac ggcccgcggc ggcgacgaaa
      121 acaacgacca ggacgctcgg cgcccctgtc cgcagagtcg ccactcccgc ctcttctccg
      181 gtctctagcg acgcgggccc gcggcttcct cctttcctgc ccagttcccg cgggcctcgg
      241 ggttgcagcg gctagaagtg ccgaggagcg ccaggaggac ccggccggct gcgaggattc
      301 acgcgcccgc caccccgccc ggcccgggct tcatgtgaaa gagggacggg cctggcccca
      361 aggcccgcgc ccgactcctc gcgcgcccct ccggtccccg ggggccctgc ggcagccccc
      421 gacctggggc cgcggtgaca ggccgcgctg gggcggaggg gcgggctcgg cgggggcggc
      481 ccctccttgg gcctgcggct ctctacgtcc ctgcggagca gccggccata aagaatgctg
      541 atgggagaac cattttccta attttcaaat tgttgagctg tttgccataa tggatgatca
      601 gcaagctttg aactcaatca tgcaagattt ggctgtcctt cataaggcca gtcgaccagc
      661 attatccttg caggaaacca gaaaagcaaa atcttcatca ccaaaaaaac agaatgatgt
      721 ccgagtcaaa tttgaacata gaggagaaaa aagaatcctt cagttcccca gaccagttaa
      781 actggaagat ctgagatcta aagctaaaat tgcctttgga cagtctatgg atctacatta
      841 taccaataac gagttggtaa ttccattaac tactcaagat gacttggaca aagctgtgga
      901 actgctggat cgtagtattc atatgaagag cctcaagata ttacttgtaa taaatggaag
      961 tacacaggct actaatttag aaccattgcc atcactagaa gatttggata atacagtatt
     1021 tggagcagag aggaaaaaac ggctatctat aataggtcct actagtagag atagaagttc
     1081 tcctccccca ggttacattc cagatgaatt acaccaggtt gcccggaatg ggtcattcac
     1141 tagtatcaac agtgaaggag agttcattcc agagagcatg gaccaaatgc tggatccatt
     1201 atctttaagc agccctgaaa attctggctc aggaagttgt ccatcacttg atagtccttt
     1261 ggatggagag agctatccaa aatcacgaat gcctagggct cagagctacc cagataatca
     1321 tcaggaattt tcagactatg ataaccctat ctttgagaaa tttggaaaag gaggaacata
     1381 tccaagaagg tatcatgttt catatcatca tcaagagtat aatgatggtc gtaaaacttt
     1441 tccaagagct agaaggaccc aggggaccag cttacggtct cctgtgagtt tcagtcctac
     1501 tgatcattcc ttaagcacta gtagtggaag cagtatcttt accccagagt atgatgatag
     1561 tcgaataaga agaaggggaa gtgacataga caatcctact ttgaccgtaa tggacatcag
     1621 cccacccagc cgttcacctc gagctccgac caactggaga ttgggcaaac tgcttggcca
     1681 aggagccttt ggaagggtct acctctgtta tgatgttgat acaggaagag aattggctgt
     1741 taagcaagtt caatttgacc ccgatagtcc tgagaccagc aaggaagtaa atgcacttga
     1801 gtgtgaaatt cagttgctga aaaacttgct acatgagcga attgttcagt attatggctg
     1861 tttgagggat ccccaggaaa aaacactttc catatttatg gaatatatgc cagggggttc
     1921 aattaaggac caattaaaag catatggcgc tcttactgag aatgtgacta ggaaatacac
     1981 ccgtcagatt ctggagggtg tccattattt gcacagtaat atgattgtcc atagagatat
     2041 caaaggcgca aatatcctgc gagattcaac aggcaacgtc aaactaggag attttggggc
     2101 cagcaaacgg cttcagacca tctgtctctc agggacagga atgaagtctg tcacgggcac
     2161 accatactgg atgagccctg aagtcatcag tggagaaggc tatggaagaa aagcagacat
     2221 ctggagtgtt gcatgtactg tggtagaaat gctaactgaa aagccgcctt gggctgaatt
     2281 tgaagcaatg gctgccatct ttaaaatcgc cactcagcca acaaacccaa agctgccacc
     2341 tcatgtctca gactatactc gagatttcct caaacggatt tttgtagagg ccaaactgag
     2401 accttcagct gatgaactct taaggcacat gtttgtgcat tatcactagc agccagtaac
     2461 ctctcctgtg cctctaccta gctcccatct attcattcac cttctctctg actgcacttt
     2521 tcttttttat aaaaaaagag agatggggga gaaaaaagac aagagggaaa gtatttctct
     2581 tgattcttgg ttaaatttgt ttaataataa taatatccta aattttttat atttaatctt
     2641 tttttccctt acaagaactt gaagtttttt tttttaattt ttataatgta ctgatgtggt
     2701 tcagagagat aaagcacttt agtacatagt cactcttttt agtacaaaca aatcatttgg
     2761 aatacctaaa gattgtagag tcattccctc tatcactgac acatcagtga cgatgggaag
     2821 acatggaaaa caaggagaag aaaatgatgt ataatttgta gtttttagtg atagtattta
     2881 aaatatatcc tcatttgtgg ggttgagccc taaactttag tttagggtag gtactcaact
     2941 taaagaatat aggtttcttc ttatatctgt attctttaga tcctaacctc tgtctaccaa
     3001 cctttttgct cagtaggagt cttgatagaa gatatgaatc tctgagaggt atgtttattt
     3061 gttaatccta accagtataa taagcaaata cactataata gatccacgtt actggaatct
     3121 gtaaaccttg agggatagct ttctgcttaa aaacacacac acacacacac acacacacac
     3181 ggaaaacctt tattttaaag tcaagttgtg agcaaataga aataaaagac aaaaggacat
     3241 cactcttatc aaatgtgtga gcagtagaag agaccacatt tacagtcaat agaaataatg
     3301 aaaaaaaatt aggtgtttag tgtattttaa acagttttgt tttgttttac ttgaggggga
     3361 cgtcccaaaa ttaaaggaat ggagaaataa tcaaaatcat gtataccatc ttctatttcc
     3421 agctcctgat tccccatagg taacatccct taggagcgaa gagttcaatt agtaatgttt
     3481 atgtgttatg tcaggagatg aaacccttgt tcttaggatc acagaatact aaagcacctc
     3541 aaaaaaacag gtatcatgtg aaacagtggt tgccaaaagt ggagcgagga tgatttcact
     3601 aggcatttgg caattcttag agacatttcc ggttgtcaca attggaggga tactagtaac
     3661 atgaattggg tagaggccag ggatgttgct aaatagatta taatacacaa ggaaagcagc
     3721 ctcaaagaat tacccctccc aaaatgtcag aagtgctgag gctgagaaac cctgatgtaa
     3781 agatcagtcc cagttataaa ctgaaaacag ctatttacaa agcagtaaat gcaagaaaga
     3841 aaaggaagtc gcaaagagaa agtgatgtaa tgcaaattga ggattttaca agatcagata
     3901 ctgctgcttt aagtcaaggg tgaataatct caacaaacag caaatagcaa tcactttacc
     3961 agctgcatat tgcacccaca taggtaactc ttcattttag gaacaaactg aatttgaaca
     4021 ctgagccttt tgaactcagc catctctata tattctttcc ttaaaattca tgattttggg
     4081 caaaaaaaaa aaaaaaagca tatcaaaaca aaattttaac tactaatggg aaggttaaaa
     4141 agctgccacg tatttctagt acaatgtatc caataagagc aaggacattg ttaaatggaa
     4201 caagacagct gggaaaggcc ccactatctc taatagaatt gtgctactat aaaacatgaa
     4261 gatatcagca atttggccac ttgcagtttt tttcagcatg ttaaagcaag tgctaagtac
     4321 tttattagta ctttatttag tactttattt taaaggtcta gaaaacaaca gtcttcctgg
     4381 aggccccaga atccctttca tgggattcat aagggactca ggtattctta ttttctgcca
     4441 aaaggtgtgc ttatttaggg actcttttac gatactttgt ttctgaagtt aggtagatct
     4501 tggtttactg tgccaaggtc ctacctagca ttccctgtag gaatatttga aattccacta
     4561 gctagtctaa gaagtcaaag cacaaaatta attgtcaacg cttctctcat attcttaaaa
     4621 ttgaagcagt ttgcagggaa gaacaagaat gaggtgtccc tgccttggta aagcaactgc
     4681 caaattttgt tctagaaaga atccccaagt gtgtggatct attataattt ctttatcttt
     4741 tatgaatgag ggcatctttt ctacttgagc aagatcatgg tccttgacca gtgggctttt
     4801 ccttcatatc tgtcttgtgt agttgtgctt ttcctttggc tcgtttttgg tctgtcaggg
     4861 taaccagcag tgcacaatat gagacaaatg ctctttagag agaaatctgg tgagactgta
     4921 gaacccagag caaaactgtt tttttttcag tctctgtttt cttttctaaa tgctatagaa
     4981 aataaaggca cctgaaaaga aactactact ttaacactgc tgtggaaggc ctttgcttta
     5041 taagaaaaat attattagct atgggaaagt aatgttcttt atgtaaagac ttaaaaatag
     5101 actaatagtt tacagagtta ttatataaaa tacgatgtga atttatttca aacaagttgt
     5161 gaaggtacca aaaaccacaa taaaagttaa tatatataaa aactactaga acaaaaaagc
     5221 ttgaaggtgt gggggtggag ggtatggaag accaaaagta aatttaaaaa agaaattttt
     5281 gaaaggaaac agctttatca ggggaagtta aatataacac tccgtttttt tcttctttct
     5341 aataatgata tggatcaaca aatgcaattt gaacttttta agataactct aaatgtgttt
     5401 tgtttcttaa atgcacaatt gattcttcta tactgtaatg cttctttgct gacgctggaa
     5461 aatggtcaga ctgctgactt tgtgccttta aaatgcattc tgctttgaca gtggcagact
     5521 ttttggtgct ggagaagatt cactagcagt cttatatgtg cttttcagga aatatctttc
     5581 tacttaaggc cttgagagat tcaaagtggc atgacttagg cttggaccaa atataagatc
     5641 ttttacattg caaacaaaat gttgtattga ataaaagggt taaaaaaatc ataatgacta
     5701 gtggaaattt tgatgggttg ttaattattt aaaatgtatt tgaaagtata cagactgaaa
     5761 atttcagtat gaatatcttg aaaattttca gcacttttcc ttgtttacaa aacatctttc
     5821 taacttaaaa aaatgactat tgccttagaa agttttaaaa ggcacagtta cccttacata
     5881 acatatataa ggaattaaaa tattaatgca tttgctgaac tgtaaaagaa aattttaaac
     5941 cttgatttca gagtgaattg gattggcttt cacttgagag agtctttacc ttattcccaa
     6001 agaaactgca ttaaaaagat gaaactattt ctttagacta agtctctgct actccagctc
     6061 atatatatgt cagatctgct ctgtagtatt tctgacatgg agttgttata agatatgtga
     6121 aatcacttag aaaataaaat cagatgtgaa tagtttagca ggatgaccaa gccagatgca
     6181 gactccttgg cctatggcaa agttctgtgc cagtgatttt atttggagtc tgggaactga
     6241 tagcttgata taaaatcacc tggtgtcaat taaatataca aatctatctt ttttaataga
     6301 atgaatcttc tttgtttgtt caataattgt aacttattat gtcaggtact aaattaacat
     6361 ccttaaggag gtcacaatct aggaggaaca tgggcaaaca gttacttatt ttatggcata
     6421 cgctaactct gaaagttcag caatgatgtt ttaaaatgtt attttttatt aagggaaaag
     6481 caattttagg taatcattgg ctgttataaa cggaaatgtc actgacaaca gggattagag
     6541 taaaagtatc tccaaactag agggatcccc tccccaacca ggtcacatag catctctctc
     6601 aggaccccct cttactgcaa aacccagcct ctactcaaca gttctggtga tggggagcat
     6661 ttattccata acagtgtttt tttgttcttt tttttttttt tccccagtgt ctcttttaaa
     6721 gaaaacatac tttattttgg tctaaattgt gaaaataccc aaaacatttg atagaaattg
     6781 aactctgtca acagtgttat ttatactaag atcaggacag ttccttgaga tcatactgtt
     6841 ttattactaa gtttggcctt tgttttacaa atgtaatgtt catatttatt tgaattttaa
     6901 gattggttaa atgttaatga aaagcaatcc aattgttaat ttttagtagt gccttttctc
     6961 tgtatgcctt aattttattt tatattaata attcaagtta cccaccaaaa tgaagatttt
     7021 ttccaaaatc agtaggcttc acaagttcat ttggccttag actgtgtatt ggtccttaaa
     7081 tgtttcttaa tgcctgcctc ctcctaccct tttctacctc ctttttaaaa aaataaataa
     7141 ataaataaat aaatgttagg agagaaacat taggatttgt ctgtgataga catgctgcag
     7201 cttccactag acatgaattc ccaacacaag catagaaaga ggtattctcg ttctaaagag
     7261 acatgtaaat gttttctggg ttgaatattt gattttcaat cacattggca ctgtccaagt
     7321 tcttggcata ttatagttca gttatgattt tcatggtaga gttacaatac ttgtttttgc
     7381 cctaaagaag tcattcacat ggtttacaaa tgttaaggca atgagatagg acagtgagta
     7441 ggaaatccag ggatagcaga ttctagtcct taattctcag ctaacatttt gggtaatact
     7501 cacagaaaag ttacttttct gtgaagtgtg tgcatgtgtg catgtatgtg tgcgtttgca
     7561 tgcatgtgtg tgtgtgtgtg tgtgtggtta gagttggagg cagggatggg aaggaagaag
     7621 gggaaaggta tgagtgttca attaggtcag tttctggttt tggaagaaca actcaaatgt
     7681 atgacctact gatgacatac agtgagtgta gtcatccttg ggtaggaaca ccagtacatt
     7741 ttccagaagt agctagtaca aatgattgta taataatatt gattgtaaat tgccatactc
     7801 ttggttgcta aaggtagatt aaaattcaat ttgtgtatat gtaaaataat cttatatata
     7861 attcttaaat agaaagtaat tgattttaca tagttcttcc tgaacttttt ggaactgaaa
     7921 ttctgccaaa cattatcaaa ataagcttcc ttagcttacc aagtattcac aaggaaattc
     7981 tcttgtacct ctttttggtt ggctagggga agcatgctgt gaagcagcta ttcctactta
     8041 tagttgtctt tggcttggct ttgacttctg ccatggtttg gacaggatgc catggtaggt
     8101 gtgggaagta tcagggcctc ccatcttccc atactcacct tccagagctg ttgatggaaa
     8161 tgagctcttg ggggagccta gagattcttt tttcagtggt cctgtaagca ttcaaggttg
     8221 agcttcagaa agatgaggga ggagtcatag ctacagtaaa gaaaagaata gattttatct
     8281 gttgcctgta tacagtctca tagaattctt atttttcata attgtgcatt ttaattgata
     8341 gtcatatggt atttttgtta ataacagcaa aggcattaaa tcagtaccat tgttccttct
     8401 gcctgatgac ttcttgtttg taacacaatc tgtattatca gaatcacttt aaaccaaagc
     8461 ttttatattc cctgtacaca tagccaaaat aattagcact gttacttctg tccacttgta
     8521 agctaatagt ataaaaatta taaagcagat actagtagaa tttgtattct ggtatatcct
     8581 tcaaggaaaa ggacaggtga tcagtgcatt tagacatcca gaagttactt ccactattga
     8641 ggaactatgt agacatgaat ttaagcttgt acatgtgcca atcttgttaa tgtcacttga
     8701 ccactgattc acatagacag acattaacgt ctgatgcctc aggttgatgg cgaactttct
     8761 tcctagtgtc ttaacattta tgaacttatg tttgacttga ggggcatttc agcaaagctg
     8821 tgaggaaaag taggttctgg actgatgtct gctttgtgcc atagacattt tggaacataa
     8881 ggaagtatct gcttctacta tatgtacagg acttgtcccc aaataatttc tgaatccctt
     8941 aatgcagttg agaatccctt aatgcagttg agaacagtaa agttcatgac cataccgaaa
     9001 agcactttta agttcacttc tttttaaata tttttttaga atttattttt taaaagcata
     9061 tgtaactagt gatgatttta gcattagctt tacttggagg ggagaagaaa caaaattaca
     9121 ccttaaactc tattagatag actacaaaat ggatttgatg tattcttact tttccaaaga
     9181 attttttcca cctggaataa gaagagagga ctagttgaaa catttgagct ggagaggctt
     9241 atgggcaata tgaagtgggc caaattcttg caatacaccc tattcaattg aaagtacagt
     9301 gtctaaggat aagaatactt ctttaaagac caacaattgt tttaatgcaa tcttaattag
     9361 taaatggttt acattttttc tattgttatt ggttttaaaa tttgctaccc tgaattctga
     9421 aatattataa gagttaatac caaaaatact tttaatgtct gacatctctt acatttcaca
     9481 tggttctttt tattcattgt aaataaaagt aaacttggat aaatttgaga ataaactttg
     9541 aattgttgca aactatgatt atcaatttgt aaatttaccc tttgacataa taatgtacat
     9601 ttttaaatgt agccattaaa ttctctatat ttaacctatt ggtttctctc taaatgtttt
     9661 taatctcagt tgaatgctgg gcagtttata attatgtaca aagggttttt ttcctattaa
     9721 gattgacatt tttgcacatt tttggaaatg tttaatttgt acactgattt actactctga
     9781 ccaataattg ttgatgagtg tatgagcatc atgaatgcgt gcattgaact actgtctgtg
     9841 tctttatgtt ttaacttctt tcaagatgta gccatcagtt actgcactgc tattactgac
     9901 ttaaatggac ctttttgctt ttacaaacta tacttatcta tggttctttg ttgaagaact
     9961 caggagttcg acattaatag aaattagaaa attttataat gtattataaa atccattact
    10021 aagttgaaag tatgctttta actgtgtatc aggggatgta tgtttgtgtg gagatgtttt
    10081 tttctgtctg taggcacatg tatatatccc agtaagttct tattttcatt tcatttctac
    10141 atttttatga acttgtttta taagggtact gtttagttgt gataattaaa tatatataaa
    10201 agctttctga gagattattt actatatgaa tatattttca gctggctgat caaaagtatt
    10261 tttaaaattt tgtttttagc cattcaaaat gtatttgtat tttcagaatt gaatgcaagt
    10321 catacttaga gctcaattaa aatactcttg caagaaaaag cattaaattc tctttgagaa
    10381 ttccgtgaaa tgcaaattaa gggaaaaata ggttttaagc agtagcaaaa acacaccgct
    10441 aaaaatggcc tttgaattct cagagctatg gaaaaatgtg agaaactaaa ttgtctcgag
    10501 atgtcaagat ttttctatct ccagttgcta ttggcctgtc ctactgttgt ctttgaatat
    10561 ttcctgtatt ccaccccaaa agatttcaac ttttagtttt tctatcactg ggcaaggtca
    10621 gactgggttt ataaaacaat atattggcat attgtgattc tttgtaattg actataaatt
    10681 ggttttgaaa tgtccaaaca tacgttgtgt gtctttatat tgtactgtgt gttttaagta
    10741 actggcagaa aaagaattgg aaatgaaatt tccaatcaga caatgtagta ttaaagaaaa
    10801 ctttgtttta aatatgcggt accatgtaaa attactcgtg ttaggaataa agtccactgt
    10861 tgaagttgaa tgtcactaag cttattatgt taaaaggaac cagtgtgatt aggaaaacct
    10921 accagaagcc agatacagtg tggtacgtgc aacatttcat gcacattttg attttgtttg
    10981 tcattcaaaa ttgtgcttgt aaaaatgtat acaatttctg agttttataa tttctaggta
    11041 cttgtttttt cttgctggta aatagctttt ttttaaaaaa aaatctaata taaaagaaac
    11101 catgtttata ttttgttagt gatcaatgac tttgtttata tggaaatttg tatattgttg
    11161 gcacacattt tgttgaggtt tacgtgcatg aaggcctgca ataattagca caatgaaaac
    11221 tgctttttct tacatgttca tttttttgaa agattgtggt gcaaaggctt actctaataa
    11281 gtaaccttct ggactatgga atgaatataa atgaatggca ctttgagtgt taataaagct
    11341 aatatttatt tccactgtga
//
