LOCUS       NM_001001671            4879 bp    mRNA    linear   PRI 16-JUN-2023
DEFINITION  Homo sapiens mitogen-activated protein kinase kinase kinase 15
            (MAP3K15), mRNA.
ACCESSION   NM_001001671 XM_372199
VERSION     NM_001001671.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4879)
  AUTHORS   Nag A, Dhindsa RS, Mitchell J, Vasavda C, Harper AR, Vitsios D,
            Ahnmark A, Bilican B, Madeyski-Bengtson K, Zarrouki B, Zoghbi AW,
            Wang Q, Smith KR, Alegre-Diaz J, Kuri-Morales P, Berumen J,
            Tapia-Conyer R, Emberson J, Torres JM, Collins R, Smith DM, Challis
            B, Paul DS, Bohlooly-Y M, Snowden M, Baker D, Fritsche-Danielson R,
            Pangalos MN and Petrovski S.
  TITLE     Human genetics uncovers MAP3K15 as an obesity-independent
            therapeutic target for diabetes
  JOURNAL   Sci Adv 8 (46), eadd5430 (2022)
   PUBMED   36383675
  REMARK    GeneRIF: Human genetics uncovers MAP3K15 as an obesity-independent
            therapeutic target for diabetes.
REFERENCE   2  (bases 1 to 4879)
  AUTHORS   Watanabe K, Morishita K, Zhou X, Shiizaki S, Uchiyama Y, Koike M,
            Naguro I and Ichijo H.
  TITLE     Cells recognize osmotic stress through liquid-liquid phase
            separation lubricated with poly(ADP-ribose)
  JOURNAL   Nat Commun 12 (1), 1353 (2021)
   PUBMED   33649309
  REMARK    GeneRIF: Macromolecular crowding induces liquid-demixing
            condensates of ASK3 under hyperosmotic stress, which transduce
            osmosensing signal into ASK3 inactivation.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 4879)
  AUTHORS   Trevelyan SJ, Brewster JL, Burgess AE, Crowther JM, Cadell AL,
            Parker BL, Croucher DR, Dobson RCJ, Murphy JM and Mace PD.
  TITLE     Structure-based mechanism of preferential complex formation by
            apoptosis signal-regulating kinases
  JOURNAL   Sci Signal 13 (622) (2020)
   PUBMED   32156783
  REMARK    GeneRIF: Structure-based mechanism of preferential complex
            formation by apoptosis signal-regulating kinases.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 4879)
  AUTHORS   Watanabe K, Umeda T, Niwa K, Naguro I and Ichijo H.
  TITLE     A PP6-ASK3 Module Coordinates the Bidirectional Cell Volume
            Regulation under Osmotic Stress
  JOURNAL   Cell Rep 22 (11), 2809-2817 (2018)
   PUBMED   29539411
  REMARK    GeneRIF: ASK3 controls both RVD and RVI under osmotic stress.
REFERENCE   5  (bases 1 to 4879)
  AUTHORS   Maruyama J, Kobayashi Y, Umeda T, Vandewalle A, Takeda K, Ichijo H
            and Naguro I.
  TITLE     Osmotic stress induces the phosphorylation of WNK4 Ser575 via the
            p38MAPK-MK pathway
  JOURNAL   Sci Rep 6, 18710 (2016)
   PUBMED   26732173
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 4879)
  AUTHORS   Naguro I, Umeda T, Kobayashi Y, Maruyama J, Hattori K, Shimizu Y,
            Kataoka K, Kim-Mitsuyama S, Uchida S, Vandewalle A, Noguchi T,
            Nishitoh H, Matsuzawa A, Takeda K and Ichijo H.
  TITLE     ASK3 responds to osmotic stress and regulates blood pressure by
            suppressing WNK1-SPAK/OSR1 signaling in the kidney
  JOURNAL   Nat Commun 3, 1285 (2012)
   PUBMED   23250415
REFERENCE   7  (bases 1 to 4879)
  AUTHORS   Kaji T, Yoshida S, Kawai K, Fuchigami Y, Watanabe W, Kubodera H and
            Kishimoto T.
  TITLE     ASK3, a novel member of the apoptosis signal-regulating kinase
            family, is essential for stress-induced cell death in HeLa cells
  JOURNAL   Biochem Biophys Res Commun 395 (2), 213-218 (2010)
   PUBMED   20362554
  REMARK    GeneRIF: 'ASK3 gene' is a novel member of apoptosis
            signal-regulating kinases and that it plays a pivotal role in the
            signal transduction pathway implicated in apoptotic cell death
            triggered by cellular stresses.
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL732423.12 and AL732326.4.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Sep 30, 2020 this sequence version replaced NM_001001671.3.
            
            Summary: The protein encoded by this gene is a member of the
            mitogen-activated protein kinase (MAPK) family. These family
            members function in a protein kinase signal transduction cascade,
            where an activated MAPK kinase kinase (MAP3K) phosphorylates and
            activates a specific MAPK kinase (MAP2K), which then activates a
            specific MAPK. This MAP3K protein plays an essential role in
            apoptotic cell death triggered by cellular stresses. [provided by
            RefSeq, Jul 2010].
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR18074969.580101.1,
                                           SRR14038192.3016630.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000338883.9/ ENSP00000345629.4
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-608               AL732423.12        114909-115516       c
            609-748             AL732423.12        88836-88975         c
            749-772             AL732423.12        86490-86513         c
            773-966             AL732423.12        64221-64414         c
            967-1135            AL732423.12        59993-60161         c
            1136-1242           AL732423.12        56921-57027         c
            1243-1413           AL732423.12        31446-31616         c
            1414-1526           AL732423.12        26239-26351         c
            1527-1686           AL732423.12        25539-25698         c
            1687-1837           AL732423.12        15115-15265         c
            1838-1945           AL732423.12        13365-13472         c
            1946-1995           AL732423.12        9932-9981           c
            1996-2091           AL732423.12        7196-7291           c
            2092-2179           AL732326.4         157004-157091       c
            2180-2313           AL732326.4         154654-154787       c
            2314-2441           AL732326.4         151509-151636       c
            2442-2572           AL732326.4         148771-148901       c
            2573-2678           AL732326.4         148430-148535       c
            2679-2836           AL732326.4         136548-136705       c
            2837-3020           AL732326.4         130905-131088       c
            3021-3180           AL732326.4         129964-130123       c
            3181-3355           AL732326.4         129081-129255       c
            3356-3541           AL732326.4         127773-127958       c
            3542-3647           AL732326.4         127387-127492       c
            3648-3813           AL732326.4         125482-125647       c
            3814-3926           AL732326.4         119166-119278       c
            3927-4027           AL732326.4         117921-118021       c
            4028-4104           AL732326.4         117767-117843       c
            4105-4879           AL732326.4         116487-117261       c
FEATURES             Location/Qualifiers
     source          1..4879
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="X"
                     /map="Xp22.12"
     gene            1..4879
                     /gene="MAP3K15"
                     /gene_synonym="ASK3; bA723P2.3"
                     /note="mitogen-activated protein kinase kinase kinase 15"
                     /db_xref="GeneID:389840"
                     /db_xref="HGNC:HGNC:31689"
                     /db_xref="MIM:300820"
     exon            1..608
                     /gene="MAP3K15"
                     /gene_synonym="ASK3; bA723P2.3"
                     /inference="alignment:Splign:2.1.0"
     CDS             248..4189
                     /gene="MAP3K15"
                     /gene_synonym="ASK3; bA723P2.3"
                     /EC_number="2.7.11.25"
                     /note="apoptosis signal-regulating kinase 3; MEKK 15; MEK
                     kinase 15; MAPK/ERK kinase kinase 15"
                     /codon_start=1
                     /product="mitogen-activated protein kinase kinase kinase
                     15"
                     /protein_id="NP_001001671.3"
                     /db_xref="CCDS:CCDS35212.2"
                     /db_xref="GeneID:389840"
                     /db_xref="HGNC:HGNC:31689"
                     /db_xref="MIM:300820"
                     /translation="MESGGGNAPAGALGAASESPQCPPPPGVEGAAGPAEPDGAAEGA
                     AGGSGEGESGGGPRRALRAVYVRSESSQGGAAGGPEAGARQCLLRACEAEGAHLTSVP
                     FGELDFGETAVLDAFYDADVAVVDMSDVSRQPSLFYHLGVRESFDMANNVILYHDTDA
                     DTALSLKDMVTQKNTASSGNYYFIPYIVTPCADYFCCESDAQRRASEYMQPNWDNILG
                     PLCMPLVDRFISLLKDIHVTSCVYYKETLLNDIRKAREKYQGEELAKELARIKLRMDN
                     TEVLTSDIIINLLLSYRDIQDYDAMVKLVETLEMLPTCDLADQHNIKFHYAFALNRRN
                     STGDREKALQIMLQVLQSCDHPGPDMFCLCGRIYKDIFLDSDCKDDTSRDSAIEWYRK
                     GFELQSSLYSGINLAVLLIVAGQQFETSLELRKIGVRLNSLLGRKGSLEKMNNYWDVG
                     QFFSVSMLAHDVGKAVQAAERLFKLKPPVWYLRSLVQNLLLIRRFKKTIIEHSPRQER
                     LNFWLDIIFEATNEVTNGLRFPVLVIEPTKVYQPSYVSINNEAEERTVSLWHVSPTEM
                     KQMHEWNFTASSIKGISLSKFDERCCFLYVHDNSDDFQIYFSTEEQCSRFFSLVKEMI
                     TNTAGSTVELEGETDGDTLEYEYDHDANGERVVLGKGTYGIVYAGRDLSNQVRIAIKE
                     IPERDSRYSQPLHEEIALHKYLKHRNIVQYLGSVSENGYIKIFMEQVPGGSLSALLRS
                     KWGPMKEPTIKFYTKQILEGLKYLHENQIVHRDIKGDNVLVNTYSGVVKISDFGTSKR
                     LAGVNPCTETFTGTLQYMAPEIIDQGPRGYGAPADIWSLGCTIIEMATSKPPFHELGE
                     PQAAMFKVGMFKIHPEIPEALSAEARAFILSCFEPDPHKRATTAELLREGFLRQVNKG
                     KKNRIAFKPSEGPRGVVLALPTQGEPMATSSSEHGSVSPDSDAQPDALFERTRAPRHH
                     LGHLLSVPDESSALEDRGLASSPEDRDQGLFLLRKDSERRAILYKILWEEQNQVASNL
                     QECVAQSSEELHLSVGHIKQIIGILRDFIRSPEHRVMATTISKLKVDLDFDSSSISQI
                     HLVLFGFQDAVNKILRNHLIRPHWMFAMDNIIRRAVQAAVTILIPELRAHFEPTCETE
                     GVDKDMDEAEEGYPPATGPGQEAQPHQQHLSLQLGELRQETNRLLEHLVEKEREYQNL
                     LRQTLEQKTQELYHLQLKLKSNCITENPAGPYGQRTDKELIDWLRLQGADAKTIEKIV
                     EEGYTLSDILNEITKEDLRYLRLRGGLLCRLWSAVSQYRRAQEASETKDKA"
     exon            609..748
                     /gene="MAP3K15"
                     /gene_synonym="ASK3; bA723P2.3"
                     /inference="alignment:Splign:2.1.0"
     exon            749..772
                     /gene="MAP3K15"
                     /gene_synonym="ASK3; bA723P2.3"
                     /inference="alignment:Splign:2.1.0"
     exon            773..966
                     /gene="MAP3K15"
                     /gene_synonym="ASK3; bA723P2.3"
                     /inference="alignment:Splign:2.1.0"
     exon            967..1135
                     /gene="MAP3K15"
                     /gene_synonym="ASK3; bA723P2.3"
                     /inference="alignment:Splign:2.1.0"
     exon            1136..1242
                     /gene="MAP3K15"
                     /gene_synonym="ASK3; bA723P2.3"
                     /inference="alignment:Splign:2.1.0"
     exon            1243..1413
                     /gene="MAP3K15"
                     /gene_synonym="ASK3; bA723P2.3"
                     /inference="alignment:Splign:2.1.0"
     exon            1414..1526
                     /gene="MAP3K15"
                     /gene_synonym="ASK3; bA723P2.3"
                     /inference="alignment:Splign:2.1.0"
     exon            1527..1686
                     /gene="MAP3K15"
                     /gene_synonym="ASK3; bA723P2.3"
                     /inference="alignment:Splign:2.1.0"
     exon            1687..1837
                     /gene="MAP3K15"
                     /gene_synonym="ASK3; bA723P2.3"
                     /inference="alignment:Splign:2.1.0"
     exon            1838..1945
                     /gene="MAP3K15"
                     /gene_synonym="ASK3; bA723P2.3"
                     /inference="alignment:Splign:2.1.0"
     exon            1946..1995
                     /gene="MAP3K15"
                     /gene_synonym="ASK3; bA723P2.3"
                     /inference="alignment:Splign:2.1.0"
     exon            1996..2091
                     /gene="MAP3K15"
                     /gene_synonym="ASK3; bA723P2.3"
                     /inference="alignment:Splign:2.1.0"
     exon            2092..2179
                     /gene="MAP3K15"
                     /gene_synonym="ASK3; bA723P2.3"
                     /inference="alignment:Splign:2.1.0"
     exon            2180..2313
                     /gene="MAP3K15"
                     /gene_synonym="ASK3; bA723P2.3"
                     /inference="alignment:Splign:2.1.0"
     exon            2314..2441
                     /gene="MAP3K15"
                     /gene_synonym="ASK3; bA723P2.3"
                     /inference="alignment:Splign:2.1.0"
     exon            2442..2572
                     /gene="MAP3K15"
                     /gene_synonym="ASK3; bA723P2.3"
                     /inference="alignment:Splign:2.1.0"
     exon            2573..2678
                     /gene="MAP3K15"
                     /gene_synonym="ASK3; bA723P2.3"
                     /inference="alignment:Splign:2.1.0"
     exon            2679..2836
                     /gene="MAP3K15"
                     /gene_synonym="ASK3; bA723P2.3"
                     /inference="alignment:Splign:2.1.0"
     exon            2837..3020
                     /gene="MAP3K15"
                     /gene_synonym="ASK3; bA723P2.3"
                     /inference="alignment:Splign:2.1.0"
     exon            3021..3180
                     /gene="MAP3K15"
                     /gene_synonym="ASK3; bA723P2.3"
                     /inference="alignment:Splign:2.1.0"
     exon            3181..3355
                     /gene="MAP3K15"
                     /gene_synonym="ASK3; bA723P2.3"
                     /inference="alignment:Splign:2.1.0"
     exon            3356..3541
                     /gene="MAP3K15"
                     /gene_synonym="ASK3; bA723P2.3"
                     /inference="alignment:Splign:2.1.0"
     exon            3542..3647
                     /gene="MAP3K15"
                     /gene_synonym="ASK3; bA723P2.3"
                     /inference="alignment:Splign:2.1.0"
     exon            3648..3813
                     /gene="MAP3K15"
                     /gene_synonym="ASK3; bA723P2.3"
                     /inference="alignment:Splign:2.1.0"
     exon            3814..3926
                     /gene="MAP3K15"
                     /gene_synonym="ASK3; bA723P2.3"
                     /inference="alignment:Splign:2.1.0"
     exon            3927..4027
                     /gene="MAP3K15"
                     /gene_synonym="ASK3; bA723P2.3"
                     /inference="alignment:Splign:2.1.0"
     exon            4028..4104
                     /gene="MAP3K15"
                     /gene_synonym="ASK3; bA723P2.3"
                     /inference="alignment:Splign:2.1.0"
     exon            4105..4879
                     /gene="MAP3K15"
                     /gene_synonym="ASK3; bA723P2.3"
                     /inference="alignment:Splign:2.1.0"
     polyA_site      4332
                     /gene="MAP3K15"
                     /gene_synonym="ASK3; bA723P2.3"
     regulatory      4366..4371
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MAP3K15"
                     /gene_synonym="ASK3; bA723P2.3"
                     /note="hexamer: TATAAA"
     polyA_site      4396
                     /gene="MAP3K15"
                     /gene_synonym="ASK3; bA723P2.3"
     regulatory      4860..4865
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MAP3K15"
                     /gene_synonym="ASK3; bA723P2.3"
                     /note="hexamer: AATAAA"
     polyA_site      4879
                     /gene="MAP3K15"
                     /gene_synonym="ASK3; bA723P2.3"
                     /note="major polyA site"
ORIGIN      
        1 ctctttctct ccggccttca aagggcgctg ggggccttgg ggcctgagcg tcaggctgca
       61 gcctaggggt actgccgtct tcggcccgcc gcggggtccc cggtgcgctc cgttccctcg
      121 attcccgtag cgtcccgcgc ttcaggagca ggtgccggga ccgcctgcgc ggctgctgta
      181 acttgtgcct cctagcggat cgcagccgct ggccactcgc ccaggggaag ggcgcgcagg
      241 cgggcacatg gagagcggcg gtgggaatgc tccggccggg gccctcgggg cggcgagcga
      301 gtcccctcag tgcccgccgc cgccgggggt ggagggcgcg gccgggccgg cggagcccga
      361 cggggcggcg gagggcgcgg caggcggcag cggcgagggc gagagtgggg gcgggccgcg
      421 gcgggctctg cgggcagtat acgtgcgcag tgagagctcc cagggcggcg cggccggcgg
      481 cccggaggct ggggcgcggc agtgcctgct gcgggcctgc gaggccgagg gcgctcacct
      541 cacctccgtg cccttcgggg agctggactt cggggagacg gccgtgctcg acgccttcta
      601 cgacgcagat gttgctgtgg tagacatgag cgatgtctcc agacagcctt ccctcttcta
      661 ccatcttgga gtccgagaaa gctttgacat ggccaataat gtgatcttgt accatgacac
      721 cgatgccgac actgctctct ctttgaagga catggtaact caaaaaaaca cagcatccag
      781 tggaaattat tatttcatcc catacatcgt gacaccgtgc gctgattatt tttgctgcga
      841 gagtgatgcc cagagacgag cctccgagta catgcagccc aactgggaca acatcctggg
      901 cccgctgtgc atgcctttgg tggacaggtt cattagcctc cttaaggaca tccacgtgac
      961 ctcatgtgtt tattacaaag aaaccttgtt aaatgacatc cggaaagcca gagagaaata
     1021 ccaaggtgag gaactggcga aggagctagc tcggatcaag ctccgcatgg ataatactga
     1081 ggttctgacc tcagacatca tcattaactt actcctgtcc taccgtgata tccaggacta
     1141 tgatgcgatg gtgaagctgg tggaaacact ggagatgctg cctacgtgtg atttggccga
     1201 tcagcataac attaaattcc actatgcgtt tgcactgaat aggagaaaca gcacaggtga
     1261 ccgtgagaag gctctgcaga tcatgctcca ggttctgcag agctgtgatc acccgggccc
     1321 cgacatgttc tgcctgtgtg ggaggatcta caaggacatc ttcttggatt cagactgcaa
     1381 agatgacacc agccgcgaca gcgccattga gtggtatcgc aaagggtttg aactccagtc
     1441 atccctctat tcgggaatta atcttgcagt tttgctgatt gttgctggac aacaatttga
     1501 aacttccttg gaactaagga aaataggtgt ccggctgaac agtttgttgg gaagaaaagg
     1561 gagcttggag aaaatgaaca attactggga tgtgggtcag ttcttcagcg tcagcatgct
     1621 ggcccatgat gtcgggaaag ccgtccaggc agcagagagg ttgttcaaac tgaaacctcc
     1681 agtctggtac ctgcgatcat tagttcagaa cttgttacta attcggcgct tcaagaaaac
     1741 cattattgaa cactcgccca ggcaagagcg gctgaacttc tggttagata taatttttga
     1801 ggcaacaaat gaagtcacta atggactcag atttccagtt ctggtcatag agccaaccaa
     1861 agtgtaccag ccttcttatg tttccataaa caatgaagcc gaggagagaa cagtttcttt
     1921 atggcatgtc tcacccacag aaatgaaaca gatgcacgaa tggaatttta cagcctcttc
     1981 cataaaggga ataagcctat caaagtttga tgaaaggtgt tgttttcttt atgtccatga
     2041 taattctgat gactttcaaa tctacttttc caccgaagag cagtgcagta gatttttctc
     2101 tttggtcaaa gagatgataa ccaatacagc aggcagtacg gtggagctgg agggagagac
     2161 cgatggagac accttggagt atgagtatga ccatgatgca aatggtgaga gagttgtctt
     2221 ggggaaaggc acgtatggga ttgtgtatgc tggccgagat ctgagcaatc aagtgcgaat
     2281 agccatcaaa gaaatcccgg agagagatag caggtattct cagcctctgc acgaggagat
     2341 agccctgcac aagtacctta agcaccgcaa tatcgttcag tacctgggct ctgtttcaga
     2401 gaacggctac attaagatat ttatggagca ggtgcctgga ggaagccttt ctgctcttct
     2461 gcgatccaaa tgggggccga tgaaggaacc gacaatcaag ttttacacca aacagatcct
     2521 ggagggcctt aagtatcttc atgaaaacca gatcgtgcac agagacataa agggcgataa
     2581 tgttctggtg aacacctaca gcggagtggt gaaaatctcc gattttggaa cctcgaaacg
     2641 tcttgcgggt gtgaacccct gcacagagac ttttactggc accctgcagt acatggcacc
     2701 tgagataatt gaccaagggc ctcgcggata tggtgcccca gccgatatct ggtccctggg
     2761 ctgcaccatc attgagatgg ccaccagcaa gcctccgttc catgagcttg gtgagccgca
     2821 ggcagccatg ttcaaagtgg gcatgtttaa gatccaccct gagattccag aagccctttc
     2881 agctgaagcc cgagccttca ttttatcctg tttcgagcct gacccccaca aacgtgccac
     2941 cactgctgag ctactgagag agggtttctt aaggcaggtg aacaagggca agaagaaccg
     3001 aattgccttc aagccctcag aaggtccccg cggtgtcgtc ctggccctgc ccacacaggg
     3061 agagcccatg gccaccagca gcagcgagca cggctctgtc tccccagact ccgacgccca
     3121 gcctgacgca ctctttgaga ggacccgggc gcccaggcac caccttggcc acctcctcag
     3181 tgttccagac gagagctcag ccttggaaga ccggggcttg gcctcgtccc cggaggacag
     3241 ggaccagggc ctcttcctgc tacgcaagga cagtgagcgc cgtgccatcc tgtacaaaat
     3301 cctctgggag gagcagaacc aggtggcttc caacctgcag gagtgtgtgg cccagagttc
     3361 cgaagagttg catctctcag ttggacacat caagcaaatc attgggatcc tgagggactt
     3421 catccgctcc ccagagcacc gggtgatggc gaccacaata tcaaagctca aggtggacct
     3481 ggactttgac agctcgtcca tcagtcagat tcacctggtg ctgttcggat ttcaggatgc
     3541 cgtaaataaa attttgagga accacttaat taggccccac tggatgttcg cgatggacaa
     3601 catcatccgc cgagcggtgc aggccgcggt caccattctc atcccagagc tccgagccca
     3661 ctttgagcct acctgtgaga ctgaaggggt agataaggac atggatgaag cggaagaggg
     3721 ctatccccca gccaccggac ctggccagga ggcccagccc caccagcagc acctgagcct
     3781 ccagctgggt gagctcagac aggagaccaa cagacttttg gaacacctag ttgaaaaaga
     3841 gagagagtac cagaatcttc tgcggcaaac tctagaacag aaaactcaag aattgtatca
     3901 ccttcagtta aaattaaaat cgaattgtat tacagagaac ccagcaggcc cctacgggca
     3961 gagaacagat aaagagctta tagactggtt gcggctgcaa ggagctgatg caaagacaat
     4021 tgaaaagatt gttgaagagg gttatacact ttcggatatt cttaatgaga tcactaagga
     4081 agatctaaga taccttcgac tacggggtgg tctcctctgc agactctgga gtgcggtctc
     4141 ccagtacaga agggctcagg aggcctcaga aaccaaagac aaggcttgat accaatcagc
     4201 taagctgtgg cagagtgtcc caccacgcta catgttttgt taaagcttct gttagtgtat
     4261 acacgaattc cgctgtgttt acatatttaa aaatgccatt gttcaattaa tagtttaaga
     4321 acttgtttta aatactgtcc tgagtttctt ttgaaacctg ttatttataa acatagaact
     4381 gtgtgtattg tgaaaacagt gagccttggt tttgacctcc cggaatatta ggaaattcac
     4441 ttgtagtccc agctatgcag gaggctgagg tgggaggatt gcttgagccc aggaggtgtg
     4501 gaggctgcag tgagccatga tcacaccact gcactccagc ctgggcaaca gagcccgacc
     4561 ctgtctcaaa aaaagtacac ccttcagcac ttgctggaat ggtgaaacaa acaaggggta
     4621 tttaacaaac atggaagctg ggacactgcc tcagaactgg tatggtactt caatttgaga
     4681 aacacaaaac tgatacgaat gtgccttgta gttaatgttt gatatgaaca gaaaatagct
     4741 tcatatttat actgaatgtg taagtagaga aaactaagtt atgtggcctt tgaaatgatt
     4801 acaaaattgg aatgattaca aaagtcttat tttaaaatgg aactgtcctc ttgcctgata
     4861 ataaatattg tatcttgta
//
