LOCUS       NM_004721               9857 bp    mRNA    linear   PRI 05-APR-2024
DEFINITION  Homo sapiens mitogen-activated protein kinase kinase kinase 13
            (MAP3K13), transcript variant 1, mRNA.
ACCESSION   NM_004721
VERSION     NM_004721.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 9857)
  AUTHORS   Liu,Z., Zhou,X., Zheng,P., Bu,C., Yan,X., Yu,H. and Xu,Y.
  TITLE     Clinical significance of mitogen-activated protein kinase kinase
            kinases in hepatitis B virus -related hepatocellular carcinoma and
            underlying mechanism exploration
  JOURNAL   Bioengineered 13 (3), 6819-6838 (2022)
   PUBMED   35311629
  REMARK    GeneRIF: Clinical significance of mitogen-activated protein kinase
            kinase kinases in hepatitis B virus -related hepatocellular
            carcinoma and underlying mechanism exploration.
REFERENCE   2  (bases 1 to 9857)
  AUTHORS   Zhang,Q., Li,X., Cui,K., Liu,C., Wu,M., Prochownik,E.V. and Li,Y.
  TITLE     The MAP3K13-TRIM25-FBXW7alpha axis affects c-Myc protein stability
            and tumor development
  JOURNAL   Cell Death Differ 27 (2), 420-433 (2020)
   PUBMED   31186535
  REMARK    GeneRIF: The MAP3K13-TRIM25-FBXW7alpha axis affects c-Myc protein
            stability and tumor development.
REFERENCE   3  (bases 1 to 9857)
  AUTHORS   Welsbie,D.S., Mitchell,K.L., Jaskula-Ranga,V., Sluch,V.M., Yang,Z.,
            Kim,J., Buehler,E., Patel,A., Martin,S.E., Zhang,P.W., Ge,Y.,
            Duan,Y., Fuller,J., Kim,B.J., Hamed,E., Chamling,X., Lei,L.,
            Fraser,I.D.C., Ronai,Z.A., Berlinicke,C.A. and Zack,D.J.
  TITLE     Enhanced Functional Genomic Screening Identifies Novel Mediators of
            Dual Leucine Zipper Kinase-Dependent Injury Signaling in Neurons
  JOURNAL   Neuron 94 (6), 1142-1154 (2017)
   PUBMED   28641113
  REMARK    GeneRIF: LZK cooperates with DLK to promote retinal ganglion cell
            death in response to axon injury.
REFERENCE   4  (bases 1 to 9857)
  AUTHORS   Han,H., Chen,Y., Cheng,L., Prochownik,E.V. and Li,Y.
  TITLE     microRNA-206 impairs c-Myc-driven cancer in a synthetic lethal
            manner by directly inhibiting MAP3K13
  JOURNAL   Oncotarget 7 (13), 16409-16419 (2016)
   PUBMED   26918941
  REMARK    GeneRIF: Data indicate that miR-206's effect on Myc was mediated
            through MAP3K13.
REFERENCE   5  (bases 1 to 9857)
  AUTHORS   Wilk,J.B., Shrine,N.R., Loehr,L.R., Zhao,J.H., Manichaikul,A.,
            Lopez,L.M., Smith,A.V., Heckbert,S.R., Smolonska,J., Tang,W.,
            Loth,D.W., Curjuric,I., Hui,J., Cho,M.H., Latourelle,J.C.,
            Henry,A.P., Aldrich,M., Bakke,P., Beaty,T.H., Bentley,A.R.,
            Borecki,I.B., Brusselle,G.G., Burkart,K.M., Chen,T.H., Couper,D.,
            Crapo,J.D., Davies,G., Dupuis,J., Franceschini,N., Gulsvik,A.,
            Hancock,D.B., Harris,T.B., Hofman,A., Imboden,M., James,A.L.,
            Khaw,K.T., Lahousse,L., Launer,L.J., Litonjua,A., Liu,Y.,
            Lohman,K.K., Lomas,D.A., Lumley,T., Marciante,K.D., McArdle,W.L.,
            Meibohm,B., Morrison,A.C., Musk,A.W., Myers,R.H., North,K.E.,
            Postma,D.S., Psaty,B.M., Rich,S.S., Rivadeneira,F., Rochat,T.,
            Rotter,J.I., Soler Artigas,M., Starr,J.M., Uitterlinden,A.G.,
            Wareham,N.J., Wijmenga,C., Zanen,P., Province,M.A., Silverman,E.K.,
            Deary,I.J., Palmer,L.J., Cassano,P.A., Gudnason,V., Barr,R.G.,
            Loos,R.J., Strachan,D.P., London,S.J., Boezen,H.M.,
            Probst-Hensch,N., Gharib,S.A., Hall,I.P., O'Connor,G.T., Tobin,M.D.
            and Stricker,B.H.
  TITLE     Genome-wide association studies identify CHRNA5/3 and HTR4 in the
            development of airflow obstruction
  JOURNAL   Am J Respir Crit Care Med 186 (7), 622-632 (2012)
   PUBMED   22837378
REFERENCE   6  (bases 1 to 9857)
  AUTHORS   Ikeda,A., Sakuma,H., Masaki,M., Kozutsumi,Y., Oka,S., Inazawa,J.
            and Kawasaki,T.
  TITLE     Genomic organization and fine-mapping of the human leucine
            zipper-bearing kinase (LZK) gene
  JOURNAL   J Biochem Mol Biol Biophys 6 (2), 113-117 (2002)
   PUBMED   12186766
  REMARK    GeneRIF: Chromosomal localization studies involving FISH mapping
            demonstrated that the human LZK gene is located at 3q27.
REFERENCE   7  (bases 1 to 9857)
  AUTHORS   Ikeda,A., Hasegawa,K., Masaki,M., Moriguchi,T., Nishida,E.,
            Kozutsumi,Y., Oka,S. and Kawasaki,T.
  TITLE     Mixed lineage kinase LZK forms a functional signaling complex with
            JIP-1, a scaffold protein of the c-Jun NH(2)-terminal kinase
            pathway
  JOURNAL   J Biochem 130 (6), 773-781 (2001)
   PUBMED   11726277
REFERENCE   8  (bases 1 to 9857)
  AUTHORS   Ikeda,A., Masaki,M., Kozutsumi,Y., Oka,S. and Kawasaki,T.
  TITLE     Identification and characterization of functional domains in a
            mixed lineage kinase LZK
  JOURNAL   FEBS Lett 488 (3), 190-195 (2001)
   PUBMED   11163770
REFERENCE   9  (bases 1 to 9857)
  AUTHORS   Sakuma,H., Ikeda,A., Oka,S., Kozutsumi,Y., Zanetta,J.P. and
            Kawasaki,T.
  TITLE     Molecular cloning and functional expression of a cDNA encoding a
            new member of mixed lineage protein kinase from human brain
  JOURNAL   J Biol Chem 272 (45), 28622-28629 (1997)
   PUBMED   9353328
REFERENCE   10 (bases 1 to 9857)
  AUTHORS   Schultz,S.J. and Nigg,E.A.
  TITLE     Identification of 21 novel human protein kinases, including 3
            members of a family related to the cell cycle regulator nimA of
            Aspergillus nidulans
  JOURNAL   Cell Growth Differ 4 (10), 821-830 (1993)
   PUBMED   8274451
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC132516.4, AB001872.1 and
            AC099661.11.
            
            On Nov 23, 2018 this sequence version replaced NM_004721.4.
            
            Summary: The protein encoded by this gene is a member of
            serine/threonine protein kinase family. This kinase contains a dual
            leucine-zipper motif, and has been shown to form dimers/oligomers
            through its leucine-zipper motif. This kinase can phosphorylate and
            activate MAPK8/JNK, MAP2K7/MKK7, which suggests a role in the JNK
            signaling pathway. [provided by RefSeq, Jul 2008].
            
            Transcript Variant: This variant (1) represents the longest
            transcript. Both variants 1 and 2 encode the same protein.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AB001872.1, SRR1660805.165372.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968540, SAMEA2142853
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000265026.8/ ENSP00000265026.3
            RefSeq Select criteria :: based on conservation, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-46                AC132516.4         15650-15695
            47-3328             AB001872.1         1-3282
            3329-9857           AC099661.11        70822-77350
FEATURES             Location/Qualifiers
     source          1..9857
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="3"
                     /map="3q27.2"
     gene            1..9857
                     /gene="MAP3K13"
                     /gene_synonym="LZK; MEKK13; MLK"
                     /note="mitogen-activated protein kinase kinase kinase 13"
                     /db_xref="GeneID:9175"
                     /db_xref="HGNC:HGNC:6852"
                     /db_xref="MIM:604915"
     exon            1..233
                     /gene="MAP3K13"
                     /gene_synonym="LZK; MEKK13; MLK"
                     /inference="alignment:Splign:2.1.0"
     exon            234..793
                     /gene="MAP3K13"
                     /gene_synonym="LZK; MEKK13; MLK"
                     /inference="alignment:Splign:2.1.0"
     CDS             319..3219
                     /gene="MAP3K13"
                     /gene_synonym="LZK; MEKK13; MLK"
                     /EC_number="2.7.11.25"
                     /note="isoform 1 is encoded by transcript variant 1;
                     leucine zipper-bearing kinase; mixed lineage kinase"
                     /codon_start=1
                     /product="mitogen-activated protein kinase kinase kinase
                     13 isoform 1"
                     /protein_id="NP_004712.1"
                     /db_xref="CCDS:CCDS3270.1"
                     /db_xref="GeneID:9175"
                     /db_xref="HGNC:HGNC:6852"
                     /db_xref="MIM:604915"
                     /translation="MANFQEHLSCSSSPHLPFSESKTFNGLQDELTAMGNHPSPKLLE
                     DQQEKGMVRTELIESVHSPVTTTVLTSVSEDSRDQFENSVLQLREHDESETAVSQGNS
                     NTVDGESTSGTEDIKIQFSRSGSGSGGFLEGLFGCLRPVWNIIGKAYSTDYKLQQQDT
                     WEVPFEEISELQWLGSGAQGAVFLGKFRAEEVAIKKVREQNETDIKHLRKLKHPNIIA
                     FKGVCTQAPCYCIIMEYCAHGQLYEVLRAGRKITPRLLVDWSTGIASGMNYLHLHKII
                     HRDLKSPNVLVTHTDAVKISDFGTSKELSDKSTKMSFAGTVAWMAPEVIRNEPVSEKV
                     DIWSFGVVLWELLTGEIPYKDVDSSAIIWGVGSNSLHLPVPSTCPDGFKILMKQTWQS
                     KPRNRPSFRQTLMHLDIASADVLATPQETYFKSQAEWREEVKKHFEKIKSEGTCIHRL
                     DEELIRRRREELRHALDIREHYERKLERANNLYMELSAIMLQLEMREKELIKREQAVE
                     KKYPGTYKRHPVRPIIHPNAMEKLMKRKGVPHKSGMQTKRPDLLRSEGIPTTEVAPTA
                     SPLSGSPKMSTSSSKSRYRSKPRHRRGNSRGSHSDFAAILKNQPAQENSPHPTYLHQA
                     QSQYPSLHHHNSLQQQYQQPPPAMSQSHHPRLNMHGQDIATCANNLRYFGPAAALRSP
                     LSNHAQRQLPGSSPDLISTAMAADCWRSSEPDKGQAGPWGCCQADAYDPCLQCRPEQY
                     GSLDIPSAEPVGRSPDLSKSPAHNPLLENAQSSEKTEENEFSGCRSESSLGTSHLGTP
                     PALPRKTRPLQKSGDDSSEEEEGEVDSEVEFPRRQRPHRCISSCQSYSTFSSENFSVS
                     DGEEGNTSDHSNSPDELADKLEDRLAEKLDDLLSQTPEIPIDISSHSDGLSDKECAVR
                     RVKTQMSLGKLCVEERGYENPMQFEESDCDSSDGECSDATVRTNKHYSSATW"
     exon            794..977
                     /gene="MAP3K13"
                     /gene_synonym="LZK; MEKK13; MLK"
                     /inference="alignment:Splign:2.1.0"
     exon            978..1169
                     /gene="MAP3K13"
                     /gene_synonym="LZK; MEKK13; MLK"
                     /inference="alignment:Splign:2.1.0"
     exon            1170..1328
                     /gene="MAP3K13"
                     /gene_synonym="LZK; MEKK13; MLK"
                     /inference="alignment:Splign:2.1.0"
     exon            1329..1487
                     /gene="MAP3K13"
                     /gene_synonym="LZK; MEKK13; MLK"
                     /inference="alignment:Splign:2.1.0"
     exon            1488..1596
                     /gene="MAP3K13"
                     /gene_synonym="LZK; MEKK13; MLK"
                     /inference="alignment:Splign:2.1.0"
     exon            1597..1706
                     /gene="MAP3K13"
                     /gene_synonym="LZK; MEKK13; MLK"
                     /inference="alignment:Splign:2.1.0"
     exon            1707..1823
                     /gene="MAP3K13"
                     /gene_synonym="LZK; MEKK13; MLK"
                     /inference="alignment:Splign:2.1.0"
     exon            1824..1961
                     /gene="MAP3K13"
                     /gene_synonym="LZK; MEKK13; MLK"
                     /inference="alignment:Splign:2.1.0"
     exon            1962..2748
                     /gene="MAP3K13"
                     /gene_synonym="LZK; MEKK13; MLK"
                     /inference="alignment:Splign:2.1.0"
     exon            2749..2819
                     /gene="MAP3K13"
                     /gene_synonym="LZK; MEKK13; MLK"
                     /inference="alignment:Splign:2.1.0"
     exon            2820..3117
                     /gene="MAP3K13"
                     /gene_synonym="LZK; MEKK13; MLK"
                     /inference="alignment:Splign:2.1.0"
     exon            3118..9857
                     /gene="MAP3K13"
                     /gene_synonym="LZK; MEKK13; MLK"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5903..5908
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MAP3K13"
                     /gene_synonym="LZK; MEKK13; MLK"
                     /note="hexamer: AATAAA"
     polyA_site      5935
                     /gene="MAP3K13"
                     /gene_synonym="LZK; MEKK13; MLK"
     regulatory      9836..9841
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MAP3K13"
                     /gene_synonym="LZK; MEKK13; MLK"
                     /note="hexamer: ATTAAA"
     polyA_site      9857
                     /gene="MAP3K13"
                     /gene_synonym="LZK; MEKK13; MLK"
                     /note="major polyA site"
ORIGIN      
        1 atgacacaca ccacagcgaa gtctgtgcgg aatcctaaac cagcccaatt tacatccatt
       61 catgaatctg tgacgtcagc aagcctttgg gctcctttgc ggtgggctgg aggattgtgt
      121 gggtggaatc cccctcccct ttatttttcc aattctgcaa ggcttttaaa attcacctta
      181 catcttttca aagcaagaaa atggaacagc atgtgtagga attcttcgtt gttgttttgg
      241 agccctctct taagtcagaa ctctgtccca aaaatcttct gagtgtcatc tcaggacttt
      301 ggttatactc atggcacgat ggccaacttt caggagcacc tgagctgctc ctcttctcca
      361 cacttaccct tcagtgaaag caaaaccttc aatggactac aagatgagct cacagctatg
      421 gggaaccacc cttctcccaa gctgctcgag gaccagcagg aaaaggggat ggtacgaaca
      481 gagctaatcg agagcgtgca cagccccgtc accacaacag tgttgacgag cgtaagtgag
      541 gattccaggg accagtttga gaacagcgtt cttcagctaa gggaacacga tgaatcagag
      601 acggcggtgt ctcaggggaa cagcaacacg gtggacggag agagcacaag cggaactgaa
      661 gacataaaga ttcagttcag caggtcaggc agtggcagtg gtgggtttct tgaaggacta
      721 tttggatgct taaggcctgt atggaatatc attgggaagg catattccac tgattacaaa
      781 ttgcagcagc aagatacttg ggaagtgcca tttgaggaga tctcagagct gcagtggctg
      841 ggtagtggag cccaaggagc ggtcttcttg ggcaagttcc gggcggaaga ggtggccatc
      901 aagaaagtga gagaacagaa tgagacggat atcaagcatt tgaggaagtt gaagcaccct
      961 aacatcatcg cattcaaggg tgtttgtact caggccccat gttattgtat tatcatggaa
     1021 tactgtgccc atggacaact ctacgaggtc ttacgagctg gcaggaagat cacacctcga
     1081 ttgctagtag actggtccac aggaattgca agtggaatga attatttgca cctccataaa
     1141 attattcatc gtgatctcaa atcacctaat gttttagtga cccacacaga tgcggtaaaa
     1201 atttcagatt ttggtacatc taaggaactc agtgacaaaa gtaccaagat gtcatttgct
     1261 ggcacggtcg catggatggc gccagaggtg atacggaatg aacctgtctc tgaaaaagtt
     1321 gatatatggt cttttggagt ggtgctttgg gagctgctga caggagagat cccttacaaa
     1381 gatgtagatt cttcagccat tatctggggt gttggaagca acagcctcca ccttccagtt
     1441 ccttccactt gccctgatgg attcaaaatc cttatgaaac agacgtggca gagtaaacct
     1501 cgaaaccgac cttcttttcg gcagacactc atgcatttag acattgcctc tgcagatgta
     1561 cttgccaccc cacaagaaac ttacttcaag tctcaggctg aatggagaga agaagtgaaa
     1621 aaacattttg agaagatcaa aagtgaagga acttgtatac accggttaga tgaagaactg
     1681 attcgaaggc gcagagaaga gctcaggcat gcgctggata ttcgtgaaca ctatgagcgg
     1741 aagcttgagc gggcgaataa tttatacatg gaattgagtg ccatcatgct gcagctagaa
     1801 atgcgggaga aggagctcat taagcgtgag caagcagtgg aaaagaagta tcctgggacc
     1861 tacaaacgac accctgttcg tcctatcatc catcccaatg ccatggagaa actcatgaaa
     1921 aggaaaggag tgcctcacaa atctgggatg cagaccaaac ggccagactt gttgagatca
     1981 gaagggatcc ccaccacaga agtggctccc actgcatccc ctttgtccgg aagtcccaaa
     2041 atgtccactt ctagcagcaa gagccgatat cgaagcaaac cacgccaccg ccgagggaat
     2101 agcagaggca gccatagtga ctttgccgca atcttgaaaa accagccagc ccaggaaaat
     2161 tcaccccatc ccacttacct gcaccaagct caatcccaat acccttctct tcatcaccat
     2221 aattctctgc agcagcaata ccagcagccc cctcctgcca tgtcccagag tcaccatccc
     2281 agactcaata tgcacggaca ggacatagca acctgcgcca acaacctgag gtatttcggc
     2341 ccagcagcag ccctgcggag cccactcagc aaccatgctc agagacagct gcccggctcg
     2401 agccctgacc tcatctccac agccatggct gcagactgct ggagaagttc tgagcctgac
     2461 aagggccaag ctggtccctg gggctgttgc caggctgacg cttatgaccc ctgccttcag
     2521 tgcaggccag aacagtatgg gtccttagac ataccctctg ctgagccagt ggggaggagc
     2581 cctgaccttt ccaagtcacc agcacataat cctctcttgg aaaacgccca gagttctgag
     2641 aaaacggaag aaaatgaatt cagcggctgt aggtctgagt catccctcgg cacctctcat
     2701 ctcggcaccc ctccagcgct acctcgaaaa acaaggcctc tgcagaagag tggagatgac
     2761 tcctcagaag aggaagaagg ggaagtagat agtgaagttg aatttccacg aagacagagg
     2821 ccccatcgct gtatcagcag ctgccagtca tattcaacct ttagctctga gaatttctct
     2881 gtgtctgatg gagaagaggg aaataccagt gaccactcaa acagtcctga tgagttagct
     2941 gataaacttg aagaccgctt ggcagagaag ctagacgacc tgctgtccca gacgccagag
     3001 attcccattg acatatcctc acactcggat gggctctctg acaaggagtg tgccgtgcgc
     3061 cgtgtgaaga ctcagatgtc tctgggcaag ctgtgtgtgg aggaacgtgg ctatgagaac
     3121 cccatgcagt ttgaagaatc ggactgtgac tcttcagatg gggagtgttc tgatgccaca
     3181 gttaggacca ataaacacta cagctctgct acctggtaat gaaggaatac acatcctgaa
     3241 gatctcgtga ctatactggc atttcagatc caccccaccc ccagactcat cccactctct
     3301 cccagcattt tgtctgggaa gagagactac cccatcttta ccacccccta gaaatgagct
     3361 gcaataacag gaacatgaga cttcgcaaat ctctggaaaa taatatccaa atgaaattaa
     3421 gtctcactga acatttcaat caagaatggc agggatctat tttattgaat attctagcta
     3481 ctgtaacatt gatatttatt tttgtttgac attttaacac tttgtactgc aaagagtgaa
     3541 ctatatatga gatagagaga caataatttc ttgcaaaaaa aaaaagagat aaaagaaaga
     3601 acagaaaaaa agaaataagt ggaatttaag agcaacatcc ttgggaattt gtggggccgg
     3661 gaggtattat tgctgcttga acaggggacc aggtcttttg gccataagtg actaaagaag
     3721 agccagagca agacattgaa cattttagaa cacaaagaac agcaaaagaa aagcaattcc
     3781 aggcaacttg tgaacagacc atattcactt caaccagctt gtccaggtgg cttacggaaa
     3841 tgacctagaa aagtctggtg accagatact tgcacccaat gtcttcaagg gcatgtgctt
     3901 cctctaggag ggaaaaacag acaaacaaac aaacaaacaa acctgtgtat gtggaagctg
     3961 gtttcatttt taaatgttta agcagcagtt ggtagtgagg tttacagata gtgtcaaatc
     4021 ttgtttttgg caaatacgtc cctttttagt gctgtcactg tcattacctt ggcaaatgtg
     4081 ctaccttgac ccaacgtgtt gatctttcat actcaagtgc catttccttg tagctcattt
     4141 cctttctgcc tgctgaccac ttcctgtagg aacaaaggtt ggggggcagt actgaggagg
     4201 agaaaagggt gagagaggaa gaacatctac agtggtgtta ggaaaacgaa cgtggaattt
     4261 ataaaactcc attctcagga tcactcagtt cagctacgag gagaagatgg aaccacccct
     4321 ctctggagcc aggttgcttg tctacttgag tcatattgat tcaagcagga gaacagactt
     4381 tgaaggcagc taggatgtat gtgtgtctat acaatgtctg agcccaaacc atccctttgt
     4441 ttttattcaa taactcattc tatcttagaa gttctttttt tttttttttt ttttgacaga
     4501 gttttgctct ttttgcccag gctagagtgc aatggcacaa tctcagttca ctgcaacctc
     4561 catctcccag gttcaagcga ttctcctgcc tcagcctccc gagtagctgg gattacaggc
     4621 gcccgcaacc acgcctggct aattttgtat ttttagtaga cacggggttt caccatgttg
     4681 gccaggcttg tctcaaactc ctgacctcag gtgatccgcc tgcctcggcc tcccaaagtg
     4741 ctgggattac aggcatcagc cactgtgccc ggcctatctt agaagtctta atgactggct
     4801 accactgtca gaataaaagc aaaaacaagc agcttgcaaa aggcaactcc tctcccagca
     4861 caatagcatt tttgttcaat gctacttgta aaatatcttt tacttcactc caaatcaatg
     4921 cagttttaaa taactggatt tgaacatttg tggaaagaac aagggatgct gagcagggat
     4981 aggaaggatt tttacattgc caaaagcatg aggtcctgcc tgtctccagg gtcatgggct
     5041 ctgaaaagca ttccaaggta cttcatggtg ccttggcctt aaggaaaaat ttttttagaa
     5101 ttttatgtac aaataggtgt tgacctagta cctgtccctg tctccaagac agattaccgt
     5161 caaacaacct ctctagattc actggactct ttgacactgc atcatgttgg acgttaggaa
     5221 atacttgcac agacagctgt taaaccattt ccaatgcaca tgaaaatgtt gccgctcctc
     5281 tgggttttac tgattgcatc agccaaaaag gaaaggcagg agggaattta gagttccttt
     5341 tgcttgtttg atccatttgt ttacacttta tgttgataca ttgatttaaa atgtagtgtc
     5401 tgtgatttat agctcttagg atgaaggaaa atgtttaatt tatacaaaga gcagtattgt
     5461 ttgcattaat ttattccatt ttgtaaaaat tctgtagctg gactacatga aagatcttaa
     5521 gttatggcat tattatcatt gttattttat tttataatat tatcattctc attttctgtc
     5581 gatcagaagg tgtgatttat gatgtggcac aatggttgtg tttattgcta accaggaatt
     5641 attatggatt ttatgatttt aatgaaggaa tgaaaatgga attcccattt gggagctccc
     5701 tggtattgat cttagctgtg ttccctaatt ttctgtgtac aacaatcatc tgaggacgca
     5761 tgttctgccc tgaagcccaa tggatacatt gtgattttga cttgatcatg aaagctcctg
     5821 ggtgggccga tgacccccag gatgtcaaat agtggatgga catatagtta aaaagctgta
     5881 aactttctaa agtttcttag gaaataaatt catgggacta tattagaaat aaaaagagaa
     5941 tgatttaatg tcctgagagg aacagaccta gagggtttcc gcacagggct catggattcg
     6001 tttcaagaat tgacaacata gtttgcaatc tccttcctgc cagacccctt tgttctccca
     6061 cctttattct cctagtggag agtagcaggg agactaaaaa aagaaatggt atacagtagt
     6121 cccctcttac ccatggcttc attttcatgg tttcagttac gctcaacagc agtccaaaaa
     6181 tatttgatgg aacattccag aaataaacaa ttcataaatt ttaattgcac agcattctga
     6241 ggagcatgat gagatcttgc cccttgctgc cctggaggtg atcctccctc tgtccagcat
     6301 attcatgctg ctcatccatt agtcacttag tagcagtctc ggtgatcaga tcgactgtct
     6361 cggttatcag atgactgtat cgcagtgctt gtgttcaagt acccctattt aattaataat
     6421 agccccaaag ggcaagagtg ctgtgcctaa tttacaaatt aaattttatc atagatatgt
     6481 aagtatagaa aaaaaaacat agtatatata gggtttggta ctatcagagt ttttaggcat
     6541 ccactggggg tcttggaatg tatcccccag aaataaggga ggactaccgt atatacatgt
     6601 agggcatgac ataaaatgcc taactgtgat gtcatcctag aacaagacta aagaacttta
     6661 agaaggaggc ctggtcactt agaggaccaa cgactggaaa aaaattcttg aaaggctata
     6721 ttttccatcc tcttgacttc agatctcact gtactgtaaa tctctctggt tcattattcc
     6781 ttggaacaga gatgctacaa ctctatgtct tctttctatc caacataaaa cactggcagt
     6841 tttgtccaat tcttgcccac ctttagtggc aacagagaaa catgagggtc agcttttatc
     6901 ttacatcttt cagtgatggt cttcctagaa aacctacccc tgatacctcg taattttgat
     6961 cagttttttt tgaattctca tcttgattat ctgttatgct attgtcatca tacaccttat
     7021 gtggattaac tgttatgagt atgttagggt agtacgtagt ggctgaatct cagtcattca
     7081 ttgtatagtc gagagttgaa taaagtccat attgaacaca tttttttaag tagaattgac
     7141 tagaggtttc agaggagaat aaggtcctta gtggaggtta aaaagggaaa taccttctct
     7201 agcccagcct ggtccttcgc tgcctgcctt gtgataaccg ggaaaatctt gaattgatgt
     7261 atgtttcttt gagaacgact ccacagtcat ctgaagagtg ttgaatcaag agctcaggga
     7321 agttatgagc taattcttca gtcctgacaa gtgtgttaca acagtgcttc caggaacctc
     7381 acaagactgt gatttcaagc tgcttcattg atggaaagtt tcaccatctt gggatgaatg
     7441 tccatgttcc gttagcgtct gtttttaact gactgcaaga tgatgagttt taatttgggt
     7501 tgcagatgag catttggttc tgattttcat ctatttcaag atcatatcct ctggataaca
     7561 gttcctttta aaattgcttt gcagaggggc tgagaagcct gcatctactt tgaaattata
     7621 atggacctta ccaccactta agtttagacc tgtttgcaag atggtgggga gctcctactg
     7681 agaaagatga agatgctagc tctattgtaa actggcgtct tcatctagct acttgaagtt
     7741 gagatactat attttaaaat gttggcatca aacaaatcta tccttctaga agtaaagaca
     7801 ttttgagtag gatttagtaa gtactggaca tctcctgtag agcttcatca tgctaaatta
     7861 ttcccctctc cttttctaaa taatagatgt gcttgggttt gggatttacc tggcaccaac
     7921 ccagactttt tttggggggg ggtgagggcg caggatctca ctctgtcacc aggttggagt
     7981 acagtggcat gattatggct cactgcagcc tcaacctcct gggctcaagt gatcctccca
     8041 cctcagcctc ctgagtagct gggaccacag gtgtgtgcca ccatgcacaa caattttttt
     8101 tttttttttg tagagatggg cgtctccctg tgttgcccag gctggtatcg aactctgggc
     8161 tctagcgatc ctcctgcttt ggcctcccaa agtactggga ttacaaacgt gagctgccat
     8221 gcccagcctc cagactattt taaatgccct ctcccactgt ggaacattgg catcctttaa
     8281 gaataaatgc atcctttttg atcaaatgat ttactctttt tttcttcaat gtcctcatgt
     8341 ctcaattttt ttttcctttt cttaaaacag tcccacttga acttcctgga actgaattct
     8401 ccagaacata atatttataa tccatagaat tcctgccctg ctctgcagta gagtgtgcta
     8461 tgttttgttt tcccactgag ccagaagccg aaatattata ctaccactta ccaccatcta
     8521 ccacctccaa gaccaagacc aacattatgt attgagcatc gtatctttcc tactgagcct
     8581 agacacagcc tcagaacctc tcaacatagc acttggagca acaattgccg cttgattgga
     8641 attgccattt gagcttgagt tagccccaat ggcagaacga caggctcgca ctagtgtcca
     8701 cctgtggctg tcaggggcgg taccacagaa aggggaaagg ccacttgcct ctacccactg
     8761 cccttttgga caacaattcc aattgaagga acaccaatca ctgatggatc ccattccaaa
     8821 atgtctcttc attcacatca tttaattctg ttgaatgaat atgttcctcc ctcacctttt
     8881 ataaacagaa aattaatata agtcccagtt acttgcttct tagactctgt gagactcatg
     8941 taccaaaaca atgtgacatt cttttcgaga ctttgacttg tacggtcagt atgaatttag
     9001 tgcctttcca ggcagcaaaa tgtttcttct ttgctgattt cataggacaa atatcctagc
     9061 atactcacac caccagtgtc ccaccctcac agactggcac attagaatca tattaatctg
     9121 cttgtttgca tgttacaact gatactgcaa aagcctattc aagtcggact gaactaggct
     9181 aagcagagga cccttgtgga acctggagca tttctcaggg acaggtggct aaagagggga
     9241 gctctaaaat cactgctctg agaaacagga ggtgggttaa ggcttttgtt aactgtcatg
     9301 tggccagggt acgatggcag gacttcacag agcagagccc taagcagatg gcaaggccac
     9361 tgtaaggaag ctcccggcag catcccaggt ctgctctcca gagccacttc acagcactca
     9421 gggatcgctg gctcccggcc ctaggctgct gtccagtgac caggtggaca gggtcctcca
     9481 gtgactgcag gccaagggaa ccccagccag catgtgcaag ctattaggaa gatgtcccat
     9541 cttgcccata tcaccccagt ggctcttcac cttcagagtg accacttccc agactctgga
     9601 agtgggaaga ccctctttga cgctttatta cagtaatttg agctcctatc gacttggagc
     9661 gaatgaattt ctcttttgtg gttaggtctc caaatgaacc tccttacctg gctcccctct
     9721 ggtctcctgt actgtcagtc cccagctccc agtgttgttt tctgatgccg gctccctctc
     9781 tgtattgaac ggcaatggga acaagaatga ctatcaaagc aaacaaatgc atggaattaa
     9841 acaaaaagtg tacttca
//
