LOCUS       NM_005921               7036 bp    mRNA    linear   PRI 07-FEB-2025
DEFINITION  Homo sapiens mitogen-activated protein kinase kinase kinase 1
            (MAP3K1), mRNA.
ACCESSION   NM_005921 XM_001128827 XM_042066
VERSION     NM_005921.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 7036)
  AUTHORS   Ganga,A.K., Sweeney,L.K., Rubio Ramos,A., Wrinn,C.M., Bishop,C.S.,
            Hamel,V., Guichard,P. and Breslow,D.K.
  TITLE     A disease-associated PPP2R3C-MAP3K1 phospho-regulatory module
            controls centrosome function
  JOURNAL   Curr Biol 34 (20), 4824-4834 (2024)
   PUBMED   39317195
  REMARK    GeneRIF: A disease-associated PPP2R3C-MAP3K1 phospho-regulatory
            module controls centrosome function.
REFERENCE   2  (bases 1 to 7036)
  AUTHORS   Wu,S., Xue,S., Tang,Y., Zhao,W., Zheng,M., Cheng,Z., Hu,X., Sun,J.
            and Ren,J.
  TITLE     Mitogen-activated protein kinase kinase kinase 1 facilitates the
            temozolomide resistance and migration of GBM via the MEK/ERK
            signalling
  JOURNAL   J Cell Mol Med 28 (20), e70173 (2024)
   PUBMED   39443331
  REMARK    GeneRIF: Mitogen-activated protein kinase kinase kinase 1
            facilitates the temozolomide resistance and migration of GBM via
            the MEK/ERK signalling.
REFERENCE   3  (bases 1 to 7036)
  AUTHORS   Kuo,S.H., Wei,M.F., Lee,Y.H., Lin,J.C., Yang,W.C., Yang,S.Y. and
            Huang,C.S.
  TITLE     MAP3K1 expression is associated with progression and poor prognosis
            of hormone receptor-positive, HER2-negative early-stage breast
            cancer
  JOURNAL   Cell Oncol (Dordr) 46 (5), 1213-1234 (2023)
   PUBMED   37166744
  REMARK    GeneRIF: MAP3K1 expression is associated with progression and poor
            prognosis of hormone receptor-positive, HER2-negative early-stage
            breast cancer.
REFERENCE   4  (bases 1 to 7036)
  AUTHORS   Wilcox,N., Dumont,M., Gonzalez-Neira,A., Carvalho,S., Joly
            Beauparlant,C., Crotti,M., Luccarini,C., Soucy,P., Dubois,S.,
            Nunez-Torres,R., Pita,G., Gardner,E.J., Dennis,J., Alonso,M.R.,
            Alvarez,N., Baynes,C., Collin-Deschesnes,A.C., Desjardins,S.,
            Becher,H., Behrens,S., Bolla,M.K., Castelao,J.E., Chang-Claude,J.,
            Cornelissen,S., Dork,T., Engel,C., Gago-Dominguez,M., Guenel,P.,
            Hadjisavvas,A., Hahnen,E., Hartman,M., Herraez,B., Jung,A.,
            Keeman,R., Kiechle,M., Li,J., Loizidou,M.A., Lush,M.,
            Michailidou,K., Panayiotidis,M.I., Sim,X., Teo,S.H., Tyrer,J.P.,
            van der Kolk,L.E., Wahlstrom,C., Wang,Q., Perry,J.R.B., Benitez,J.,
            Schmidt,M.K., Schmutzler,R.K., Pharoah,P.D.P., Droit,A.,
            Dunning,A.M., Kvist,A., Devilee,P., Easton,D.F. and Simard,J.
  CONSRTM   SGBCC Investigators
  TITLE     Exome sequencing identifies breast cancer susceptibility genes and
            defines the contribution of coding variants to breast cancer risk
  JOURNAL   Nat Genet 55 (9), 1435-1439 (2023)
   PUBMED   37592023
  REMARK    GeneRIF: Exome sequencing identifies breast cancer susceptibility
            genes and defines the contribution of coding variants to breast
            cancer risk.
            Erratum:[Nat Genet. 2023 Nov;55(11):2009. doi:
            10.1038/s41588-023-01549-x.. PMID: 37752376]
REFERENCE   5  (bases 1 to 7036)
  AUTHORS   Fatemi,N., Tu,S.J., Chung,C.C., Moghadam,P.K., Mojarad,E.N.,
            Sadeghi,A., Totonchi,M., Aghdaei,H.A. and Chang,J.G.
  TITLE     Whole exome sequencing identifies MAP3K1, MSH2, and MLH1 as
            potential cancer-predisposing genes in familial early-onset
            colorectal cancer
  JOURNAL   Kaohsiung J Med Sci 39 (9), 896-903 (2023)
   PUBMED   37314251
  REMARK    GeneRIF: Whole exome sequencing identifies MAP3K1, MSH2, and MLH1
            as potential cancer-predisposing genes in familial early-onset
            colorectal cancer.
REFERENCE   6  (bases 1 to 7036)
  AUTHORS   Fanger,G.R., Widmann,C., Porter,A.C., Sather,S., Johnson,G.L. and
            Vaillancourt,R.R.
  TITLE     14-3-3 proteins interact with specific MEK kinases
  JOURNAL   J Biol Chem 273 (6), 3476-3483 (1998)
   PUBMED   9452471
REFERENCE   7  (bases 1 to 7036)
  AUTHORS   Lee,F.S., Hagler,J., Chen,Z.J. and Maniatis,T.
  TITLE     Activation of the IkappaB alpha kinase complex by MEKK1, a kinase
            of the JNK pathway
  JOURNAL   Cell 88 (2), 213-222 (1997)
   PUBMED   9008162
REFERENCE   8  (bases 1 to 7036)
  AUTHORS   Vinik,B.S., Kay,E.S. and Fiedorek,F.T. Jr.
  TITLE     Mapping of the MEK kinase gene (Mekk) to mouse chromosome 13 and
            human chromosome 5
  JOURNAL   Mamm Genome 6 (11), 782-783 (1995)
   PUBMED   8597633
REFERENCE   9  (bases 1 to 7036)
  AUTHORS   Yan,M., Dai,T., Deak,J.C., Kyriakis,J.M., Zon,L.I., Woodgett,J.R.
            and Templeton,D.J.
  TITLE     Activation of stress-activated protein kinase by MEKK1
            phosphorylation of its activator SEK1
  JOURNAL   Nature 372 (6508), 798-800 (1994)
   PUBMED   7997270
REFERENCE   10 (bases 1 to 7036)
  AUTHORS   Mohnach,L., Fechner,P.Y. and Keegan,C.E.
  TITLE     Nonsyndromic Disorders of Testicular Development Overview
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301714
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC008937.7, AF042838.1,
            BU194120.1, DA889202.1 and AA602425.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_005921.1.
            
            Summary: The protein encoded by this gene is a serine/threonine
            kinase and is part of some signal transduction cascades, including
            the ERK and JNK kinase pathways as well as the NF-kappa-B pathway.
            The encoded protein is activated by autophosphorylation and
            requires magnesium as a cofactor in phosphorylating other proteins.
            This protein has E3 ligase activity conferred by a plant
            homeodomain (PHD) in its N-terminus and phospho-kinase activity
            conferred by a kinase domain in its C-terminus. [provided by
            RefSeq, Mar 2012].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR18074967.2674480.1,
                                           SRR14038192.508268.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000399503.4/ ENSP00000382423.3
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-507               AC008937.7         118022-118528       c
            508-1060            AF042838.1         426-978
            1061-1177           AC008937.7         68621-68737         c
            1178-1326           AC008937.7         68100-68248         c
            1327-2394           AF042838.1         1245-2312
            2395-3691           AC008937.7         51211-52507         c
            3692-4282           BU194120.1         127-717
            4283-4678           DA889202.1         164-559
            4679-6879           AC008937.7         38082-40282         c
            6880-7036           AA602425.1         11-167              c
FEATURES             Location/Qualifiers
     source          1..7036
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="5"
                     /map="5q11.2"
     gene            1..7036
                     /gene="MAP3K1"
                     /gene_synonym="MAPKKK1; MEKK; MEKK 1; MEKK1; SRXY6"
                     /note="mitogen-activated protein kinase kinase kinase 1"
                     /db_xref="GeneID:4214"
                     /db_xref="HGNC:HGNC:6848"
                     /db_xref="MIM:600982"
     exon            1..507
                     /gene="MAP3K1"
                     /gene_synonym="MAPKKK1; MEKK; MEKK 1; MEKK1; SRXY6"
                     /inference="alignment:Splign:2.1.0"
     CDS             26..4564
                     /gene="MAP3K1"
                     /gene_synonym="MAPKKK1; MEKK; MEKK 1; MEKK1; SRXY6"
                     /EC_number="2.7.11.25"
                     /EC_number="2.3.2.27"
                     /note="MAP/ERK kinase kinase 1; MEK kinase 1; MAPK/ERK
                     kinase kinase 1; mitogen-activated protein kinase kinase
                     kinase 1, E3 ubiquitin protein ligase"
                     /codon_start=1
                     /product="mitogen-activated protein kinase kinase kinase
                     1"
                     /protein_id="NP_005912.1"
                     /db_xref="CCDS:CCDS43318.1"
                     /db_xref="GeneID:4214"
                     /db_xref="HGNC:HGNC:6848"
                     /db_xref="MIM:600982"
                     /translation="MAAAAGNRASSSGFPGARATSPEAGGGGGALKASSAPAAAAGLL
                     REAGSGGRERADWRRRQLRKVRSVELDQLPEQPLFLAASPPASSTSPSPEPADAAGSG
                     TGFQPVAVPPPHGAASRGGAHLTESVAAPDSGASSPAAAEPGEKRAPAAEPSPAAAPA
                     GREMENKETLKGLHKMDDRPEERMIREKLKATCMPAWKHEWLERRNRRGPVVVKPIPV
                     KGDGSEMNHLAAESPGEVQASAASPASKGRRSPSPGNSPSGRTVKSESPGVRRKRVSP
                     VPFQSGRITPPRRAPSPDGFSPYSPEETNRRVNKVMRARLYLLQQIGPNSFLIGGDSP
                     DNKYRVFIGPQNCSCARGTFCIHLLFVMLRVFQLEPSDPMLWRKTLKNFEVESLFQKY
                     HSRRSSRIKAPSRNTIQKFVSRMSNSHTLSSSSTSTSSSENSIKDEEEQMCPICLLGM
                     LDEESLTVCEDGCRNKLHHHCMSIWAEECRRNREPLICPLCRSKWRSHDFYSHELSSP
                     VDSPSSLRAAQQQTVQQQPLAGSRRNQESNFNLTHYGTQQIPPAYKDLAEPWIQVFGM
                     ELVGCLFSRNWNVREMALRRLSHDVSGALLLANGESTGNSGGSSGSSPSGGATSGSSQ
                     TSISGDVVEACCSVLSMVCADPVYKVYVAALKTLRAMLVYTPCHSLAERIKLQRLLQP
                     VVDTILVKCADANSRTSQLSISTLLELCKGQAGELAVGREILKAGSIGIGGVDYVLNC
                     ILGNQTESNNWQELLGRLCLIDRLLLEFPAEFYPHIVSTDVSQAEPVEIRYKKLLSLL
                     TFALQSIDNSHSMVGKLSRRIYLSSARMVTTVPHVFSKLLEMLSVSSSTHFTRMRRRL
                     MAIADEVEIAEAIQLGVEDTLDGQQDSFLQASVPNNYLETTENSSPECTVHLEKTGKG
                     LCATKLSASSEDISERLASISVGPSSSTTTTTTTTEQPKPMVQTKGRPHSQCLNSSPL
                     SHHSQLMFPALSTPSSSTPSVPAGTATDVSKHRLQGFIPCRIPSASPQTQRKFSLQFH
                     RNCPENKDSDKLSPVFTQSRPLPSSNIHRPKPSRPTPGNTSKQGDPSKNSMTLDLNSS
                     SKCDDSFGCSSNSSNAVIPSDETVFTPVEEKCRLDVNTELNSSIEDLLEASMPSSDTT
                     VTFKSEVAVLSPEKAENDDTYKDDVNHNQKCKEKMEAEEEEALAIAMAMSASQDALPI
                     VPQLQVENGEDIIIIQQDTPETLPGHTKAKQPYREDTEWLKGQQIGLGAFSSCYQAQD
                     VGTGTLMAVKQVTYVRNTSSEQEEVVEALREEIRMMSHLNHPNIIRMLGATCEKSNYN
                     LFIEWMAGGSVAHLLSKYGAFKESVVINYTEQLLRGLSYLHENQIIHRDVKGANLLID
                     STGQRLRIADFGAAARLASKGTGAGEFQGQLLGTIAFMAPEVLRGQQYGRSCDVWSVG
                     CAIIEMACAKPPWNAEKHSNHLALIFKIASATTAPSIPSHLSPGLRDVALRCLELQPQ
                     DRPPSRELLKHPVFRTTW"
     exon            508..658
                     /gene="MAP3K1"
                     /gene_synonym="MAPKKK1; MEKK; MEKK 1; MEKK1; SRXY6"
                     /inference="alignment:Splign:2.1.0"
     exon            659..859
                     /gene="MAP3K1"
                     /gene_synonym="MAPKKK1; MEKK; MEKK 1; MEKK1; SRXY6"
                     /inference="alignment:Splign:2.1.0"
     exon            860..1060
                     /gene="MAP3K1"
                     /gene_synonym="MAPKKK1; MEKK; MEKK 1; MEKK1; SRXY6"
                     /inference="alignment:Splign:2.1.0"
     exon            1061..1177
                     /gene="MAP3K1"
                     /gene_synonym="MAPKKK1; MEKK; MEKK 1; MEKK1; SRXY6"
                     /inference="alignment:Splign:2.1.0"
     exon            1178..1326
                     /gene="MAP3K1"
                     /gene_synonym="MAPKKK1; MEKK; MEKK 1; MEKK1; SRXY6"
                     /inference="alignment:Splign:2.1.0"
     exon            1327..1448
                     /gene="MAP3K1"
                     /gene_synonym="MAPKKK1; MEKK; MEKK 1; MEKK1; SRXY6"
                     /inference="alignment:Splign:2.1.0"
     exon            1449..1530
                     /gene="MAP3K1"
                     /gene_synonym="MAPKKK1; MEKK; MEKK 1; MEKK1; SRXY6"
                     /inference="alignment:Splign:2.1.0"
     exon            1531..1711
                     /gene="MAP3K1"
                     /gene_synonym="MAPKKK1; MEKK; MEKK 1; MEKK1; SRXY6"
                     /inference="alignment:Splign:2.1.0"
     exon            1712..1990
                     /gene="MAP3K1"
                     /gene_synonym="MAPKKK1; MEKK; MEKK 1; MEKK1; SRXY6"
                     /inference="alignment:Splign:2.1.0"
     exon            1991..2112
                     /gene="MAP3K1"
                     /gene_synonym="MAPKKK1; MEKK; MEKK 1; MEKK1; SRXY6"
                     /inference="alignment:Splign:2.1.0"
     exon            2113..2204
                     /gene="MAP3K1"
                     /gene_synonym="MAPKKK1; MEKK; MEKK 1; MEKK1; SRXY6"
                     /inference="alignment:Splign:2.1.0"
     exon            2205..2394
                     /gene="MAP3K1"
                     /gene_synonym="MAPKKK1; MEKK; MEKK 1; MEKK1; SRXY6"
                     /inference="alignment:Splign:2.1.0"
     exon            2395..3691
                     /gene="MAP3K1"
                     /gene_synonym="MAPKKK1; MEKK; MEKK 1; MEKK1; SRXY6"
                     /inference="alignment:Splign:2.1.0"
     exon            3692..3844
                     /gene="MAP3K1"
                     /gene_synonym="MAPKKK1; MEKK; MEKK 1; MEKK1; SRXY6"
                     /inference="alignment:Splign:2.1.0"
     exon            3845..4007
                     /gene="MAP3K1"
                     /gene_synonym="MAPKKK1; MEKK; MEKK 1; MEKK1; SRXY6"
                     /inference="alignment:Splign:2.1.0"
     exon            4008..4139
                     /gene="MAP3K1"
                     /gene_synonym="MAPKKK1; MEKK; MEKK 1; MEKK1; SRXY6"
                     /inference="alignment:Splign:2.1.0"
     exon            4140..4282
                     /gene="MAP3K1"
                     /gene_synonym="MAPKKK1; MEKK; MEKK 1; MEKK1; SRXY6"
                     /inference="alignment:Splign:2.1.0"
     exon            4283..4414
                     /gene="MAP3K1"
                     /gene_synonym="MAPKKK1; MEKK; MEKK 1; MEKK1; SRXY6"
                     /inference="alignment:Splign:2.1.0"
     exon            4415..7036
                     /gene="MAP3K1"
                     /gene_synonym="MAPKKK1; MEKK; MEKK 1; MEKK1; SRXY6"
                     /inference="alignment:Splign:2.1.0"
     regulatory      7017..7022
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MAP3K1"
                     /gene_synonym="MAPKKK1; MEKK; MEKK 1; MEKK1; SRXY6"
                     /note="hexamer: AATAAA"
     polyA_site      7036
                     /gene="MAP3K1"
                     /gene_synonym="MAPKKK1; MEKK; MEKK 1; MEKK1; SRXY6"
                     /note="major polyA site"
ORIGIN      
        1 gagcgaatgt agcccgcgag agaaaatggc ggcggcggcg gggaatcgcg cctcgtcgtc
       61 gggattcccg ggcgccaggg ctacgagccc tgaggcaggc ggcggcggag gagccctcaa
      121 ggcgagcagc gcgcccgcgg ctgccgcggg actgctgcgg gaggcgggca gcgggggccg
      181 cgagcgggcg gactggcggc ggcggcagct gcgcaaagtg cggagtgtgg agctggacca
      241 gctgcctgag cagccgctct tccttgccgc ctcaccgccg gcctcctcga cttccccgtc
      301 gccggagccc gcggacgcag cggggagtgg gaccggcttc cagcctgtgg cggtgccgcc
      361 gccccacgga gccgcgagcc gcggcggcgc ccaccttacc gagtcggtgg cggcgccgga
      421 cagcggcgcc tcgagtcccg cagcggccga gcccggggag aagcgggcgc ccgccgccga
      481 gccgtctcct gcagcggccc ccgccggtcg tgagatggag aataaagaaa ctctcaaagg
      541 gttgcacaag atggatgatc gtccagagga acgaatgatc agggagaaac tgaaggcaac
      601 ctgtatgcca gcctggaagc acgaatggtt ggaaaggaga aataggcgag ggcctgtggt
      661 ggtaaaacca atcccagtta aaggagatgg atctgaaatg aatcacttag cagctgagtc
      721 tccaggagag gtccaggcaa gtgcggcttc accagcttcc aaaggccgac gcagtccttc
      781 tcctggcaac tccccatcag gtcgcacagt gaaatcagaa tctccaggag taaggagaaa
      841 aagagtttcc ccagtgcctt ttcagagtgg cagaatcaca ccaccccgaa gagccccttc
      901 accagatggc ttctcaccat atagccctga ggaaacaaac cgccgtgtta acaaagtgat
      961 gcgggccaga ctgtacttac tgcagcagat agggcctaac tctttcctga ttggaggaga
     1021 cagcccagac aataaatacc gggtgtttat tgggcctcag aactgcagct gtgcacgtgg
     1081 aacattctgt attcatctgc tatttgtgat gctccgggtg tttcaactag aaccttcaga
     1141 cccaatgtta tggagaaaaa ctttaaagaa ttttgaggtt gagagtttgt tccagaaata
     1201 tcacagtagg cgtagctcaa ggatcaaagc tccatctcgt aacaccatcc agaagtttgt
     1261 ttcacgcatg tcaaattctc atacattgtc atcatctagt acttctacgt ctagttcaga
     1321 aaacagcata aaggatgaag aggaacagat gtgtcctatt tgcttgttgg gcatgcttga
     1381 tgaagaaagt cttacagtgt gtgaagacgg ctgcaggaac aagctgcacc accactgcat
     1441 gtcaatttgg gcagaagagt gtagaagaaa tagagaacct ttaatatgtc ccctttgtag
     1501 atctaagtgg agatctcatg atttctacag ccacgagttg tcaagtcctg tggattcccc
     1561 ttcttccctc agagctgcac agcagcaaac cgtacagcag cagcctttgg ctggatcacg
     1621 aaggaatcaa gagagcaatt ttaaccttac tcattatgga actcagcaaa tccctcctgc
     1681 ttacaaagat ttagctgagc catggattca ggtgtttgga atggaactcg ttggctgctt
     1741 attttctaga aactggaatg tgagagagat ggccctcagg cgtctttccc atgatgtcag
     1801 tggggccctg ctgttggcaa atggggagag cactggaaat tctgggggca gcagtggaag
     1861 cagcccgagt gggggagcca ccagtgggtc ttcccagacc agtatctcag gagatgtggt
     1921 ggaggcatgc tgcagcgttc tgtcaatggt ctgtgctgac cctgtctaca aagtgtacgt
     1981 tgctgcttta aaaacattga gagccatgct ggtatatact ccttgccaca gtttagcgga
     2041 aagaatcaaa cttcagagac ttctccagcc agttgtagac accatcctag tcaaatgtgc
     2101 agatgccaat agccgcacaa gtcagctgtc catatcaaca ctgttggaac tgtgcaaagg
     2161 ccaagcagga gagttggcag ttggcagaga aatactaaaa gctggatcca ttggtattgg
     2221 tggtgttgat tatgtcttaa attgtattct tggaaaccaa actgaatcaa acaattggca
     2281 agaacttctt ggccgccttt gtcttataga tagactgttg ttggaatttc ctgctgaatt
     2341 ttatcctcat attgtcagta ctgatgtttc acaagctgag cctgttgaaa tcaggtataa
     2401 gaagctgctg tccctcttaa cctttgcttt gcagtccatt gataattccc actcaatggt
     2461 tggcaaactt tccagaagga tctacttgag ttctgcaaga atggttacta cagtacccca
     2521 tgtgttttca aaactgttag aaatgctgag tgtttccagt tccactcact tcaccaggat
     2581 gcgtcgccgt ttgatggcta ttgcagatga ggtggaaatt gccgaagcca tccagttggg
     2641 cgtagaagac actttggatg gtcaacagga cagcttcttg caggcatctg ttcccaacaa
     2701 ctatctggaa accacagaga acagttcccc tgagtgcaca gtccatttag agaaaactgg
     2761 aaaaggatta tgtgctacaa aattgagtgc cagttcagag gacatttctg agagactggc
     2821 cagcatttca gtaggacctt ctagttcaac aacaacaaca acaacaacaa cagagcaacc
     2881 aaagccaatg gttcaaacaa aaggcagacc ccacagtcag tgtttgaact cctctccttt
     2941 atctcatcat tcccaattaa tgtttccagc cttgtcaacc ccttcttctt ctaccccatc
     3001 tgtaccagct ggcactgcaa cagatgtctc taagcataga cttcagggat tcattccctg
     3061 cagaatacct tctgcatctc ctcaaacaca gcgcaagttt tctctacaat tccacagaaa
     3121 ctgtcctgaa aacaaagact cagataaact ttccccagtc tttactcagt caagaccctt
     3181 gccctccagt aacatacaca ggccaaagcc atctagacct accccaggta atacaagtaa
     3241 acagggagat ccctcaaaaa atagcatgac acttgatctg aacagtagtt ccaaatgtga
     3301 tgacagcttt ggctgtagca gcaatagtag taatgctgtt atacccagtg acgagacagt
     3361 gttcacccca gtagaggaga aatgcagatt agatgtcaat acagagctca actccagtat
     3421 tgaggacctt cttgaagcat ctatgccttc aagtgataca acagtaactt ttaagtcaga
     3481 agttgctgtc ctgtctcctg aaaaggctga aaatgatgat acctacaaag atgatgtgaa
     3541 tcataatcaa aagtgcaaag agaagatgga agctgaagaa gaagaagctt tagcaattgc
     3601 catggcaatg tcagcgtctc aggatgccct ccccatagtt cctcagctgc aggttgaaaa
     3661 tggagaagat atcatcatta ttcaacagga tacaccagag actctaccag gacataccaa
     3721 agcaaaacaa ccgtatagag aagacactga atggctgaaa ggtcaacaga taggccttgg
     3781 agcattttct tcttgttatc aggctcaaga tgtgggaact ggaactttaa tggctgttaa
     3841 acaggtgact tatgtcagaa acacatcttc tgagcaagaa gaagtagtag aagcactaag
     3901 agaagagata agaatgatga gccatctgaa tcatccaaac atcattagga tgttgggagc
     3961 cacgtgtgag aagagcaatt acaatctctt cattgaatgg atggcagggg gatcggtggc
     4021 tcatttgctg agtaaatatg gagccttcaa agaatcagta gttattaact acactgaaca
     4081 gttactccgt ggcctttcgt atctccatga aaaccaaatc attcacagag atgtcaaagg
     4141 tgccaatttg ctaattgaca gcactggtca gagactaaga attgcagatt ttggagctgc
     4201 agccaggttg gcatcaaaag gaactggtgc aggagagttt cagggacaat tactggggac
     4261 aattgcattt atggcacctg aggtactaag aggtcaacag tatggaagga gctgtgatgt
     4321 atggagtgtt ggctgtgcta ttatagaaat ggcttgtgca aaaccaccat ggaatgcaga
     4381 aaaacactcc aatcatcttg ctttgatatt taagattgct agtgcaacta ctgctccatc
     4441 gatcccttca catttgtctc ctggtttacg agatgtggct cttcgttgtt tagaacttca
     4501 acctcaggac agacctccat caagagagct actgaagcat ccagtctttc gtactacatg
     4561 gtagccaatt atgcagatca actacagtag aaacaggatg ctcaacaaga gaaaaaaaac
     4621 ttgtggggaa ccacattgat attctactgg ccatgatgcc actgaacagc tatgaacgag
     4681 gccagtgggg aacccttacc taagtatgtg attgacaaat catgatctgt acctaagctc
     4741 agtatgcaaa agcccaaact agtgcagaaa ctgtaaactg tgcctttcaa agaactggcc
     4801 ctaggtgaac aggaaaacaa tgaagtttgc atgactaaat tgcagaagca taattttatt
     4861 tttttggagc actttttcag caatattagc ggctgagggg ctcaggatct attttaatat
     4921 ttcaattatt cttccatttc atatagtgat cacaagcagg gggttctgca attccgttca
     4981 aattttttgt cactggctat aaaatcagta tctgcctctt ttaggtcaga gtatgctatg
     5041 agtagcaata catacatata tttttaaaag ttgatacttc tttatgaccc acagttgacc
     5101 tttattttct taaataccag ggcagttgtg gctcattgtg cattttactg ttggcccatt
     5161 catttcgttt ttggaaatta tggttttgta ttttcatgtt tatttacatt catttttgtt
     5221 tattcaggga aagctgatct ttttttcaaa ccagaaaaaa aaaatgaact agatatgaag
     5281 tagagttcat taaatatctt gctattgtca gagtttttaa aatatagact taattttgtt
     5341 tttttaaatt ggaatacaat aaagtactac ctacatttga gtcagtcacc actcttattg
     5401 tgcaggttaa gtacaagtta actaaaaata aactgtcctc tctggtgcaa ctcacaacca
     5461 agatcaagat taccttaaaa tttatttgaa ttttttagat gttttggttg tcaaactgta
     5521 ggaaacttca caacatttaa gtcttactct gtatgtaaca atccatcatt caccttcact
     5581 actggtagta acatagagct gccattttcc ttttaccatg catcatctct ttacagtagg
     5641 cctggcagat cattttttaa aaagattatt caactaccaa tcagtaatgt ttttaaacag
     5701 tacatttgct ttgaacttgg aaaatgtgtt cagaaagaaa aatggaattg aatttcattt
     5761 atacactaat tccttggatt ttgcacagtt acctaacggt tttagtctgg agttaaattc
     5821 agatgcatgg aatcctgaag gaaaatggta gctttttaat ctttttgtgt gtgtgtgagt
     5881 cttttaaatc aagtactgat taactattaa gtacaacttt gagattttag ttttaactct
     5941 tcagaagcca gtgtgaaata gaattggtta ttctcaaaga ctcaggataa actaaataag
     6001 ctatatatag agtacattta aaatgtacaa cacaaattgg aaataaaata agttacaaga
     6061 taagtttaca gggatatatt gcttacaatt tttaaaaggc agtttgtttt ttatgtgaat
     6121 atgtttctta gtgaaatttt acattccttt gttttggaag attggcgata tttgaagagt
     6181 taaaaatagt acagaaatgt gaagtttggt atctctaaat gtgttgtact tgactttctt
     6241 ttttattttg tttttttttt tttttgacta cttagaattt tcacaattct aataagattg
     6301 tttccaagtc tctcatgtgc aagctttaaa ggatgcactc ttgccatttt atgtactgga
     6361 agatcattgg tcagatgaat actgtgtctg acaaaaatgt aaactgtata aactgaggaa
     6421 cctcagctaa tcagtattac tttgtagatc accatgccca ccacatttca aactcaaact
     6481 atctgtagat ttcaaaatcc attgtgtttg agtttgtttg cagttccctc agcttgctgg
     6541 taattgtggt gttttgtttt ttgttttgtt ttcaatgcaa atgtgatgta atattcttat
     6601 tttctttgga tcaaagctgg actggaaatt gtatcgtgta attatttttg tgttcttaat
     6661 gttatttggt actcaagttg taaataacgt ctactactgt ttattccagt ttctactacc
     6721 tcaggtgtcc tatagatttt tcttctacca aagttcactt tcacaatgaa attatatttg
     6781 ctgtgtgact atgattccta agatttccag ggcttaaggg ctaacttcta ttagcacctt
     6841 actgtgtaag caaatgttac aaaaaaaaaa aaaaaaaatc tctgggttaa gaaaatttgg
     6901 cttaaatgta tcctttgtta ttttaaatat attgagatat tttaattaaa atttttaccc
     6961 cattgaaccg attttatagt atttgtacct attttggtgt ttttgtcttt atagtaaata
     7021 aaagtttttg aacaaa
//
