LOCUS       NM_002758              13405 bp    mRNA    linear   PRI 05-APR-2024
DEFINITION  Homo sapiens mitogen-activated protein kinase kinase 6 (MAP2K6),
            transcript variant 1, mRNA.
ACCESSION   NM_002758
VERSION     NM_002758.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 13405)
  AUTHORS   Chen,J., Li,J., Sun,H., Hu,T., Wang,Y., Kang,G., Cao,M. and Li,X.
  TITLE     PPM1G promotes the progression of lung adenocarcinoma by inhibiting
            p38 activation via dephosphorylation of MEK6
  JOURNAL   Carcinogenesis 44 (1), 93-104 (2023)
   PUBMED   36349938
  REMARK    GeneRIF: PPM1G promotes the progression of lung adenocarcinoma by
            inhibiting p38 activation via dephosphorylation of MEK6.
REFERENCE   2  (bases 1 to 13405)
  AUTHORS   Tu,P., Yan,S. and Zhang,F.
  TITLE     Circ_0005925 Promotes Granulosa Cell Growth by Targeting MiR-324-3p
            to Upregulate MAP2K6 in Polycystic Ovary Syndrome
  JOURNAL   Biochem Genet 61 (1), 21-34 (2023)
   PUBMED   35689709
  REMARK    GeneRIF: Circ_0005925 Promotes Granulosa Cell Growth by Targeting
            MiR-324-3p to Upregulate MAP2K6 in Polycystic Ovary Syndrome.
REFERENCE   3  (bases 1 to 13405)
  AUTHORS   Sun,Z., Chen,G., Wang,L., Sang,Q., Xu,G. and Zhang,N.
  TITLE     APEX1 promotes the oncogenicity of hepatocellular carcinoma via
            regulation of MAP2K6
  JOURNAL   Aging (Albany NY) 14 (19), 7959-7971 (2022)
   PUBMED   36205565
  REMARK    GeneRIF: APEX1 promotes the oncogenicity of hepatocellular
            carcinoma via regulation of MAP2K6.
REFERENCE   4  (bases 1 to 13405)
  AUTHORS   Robinson,K.S., Toh,G.A., Rozario,P., Chua,R., Bauernfried,S.,
            Sun,Z., Firdaus,M.J., Bayat,S., Nadkarni,R., Poh,Z.S., Tham,K.C.,
            Harapas,C.R., Lim,C.K., Chu,W., Tay,C.W.S., Tan,K.Y., Zhao,T.,
            Bonnard,C., Sobota,R., Connolly,J.E., Common,J., Masters,S.L.,
            Chen,K.W., Ho,L., Wu,B., Hornung,V. and Zhong,F.L.
  TITLE     ZAKalpha-driven ribotoxic stress response activates the human NLRP1
            inflammasome
  JOURNAL   Science 377 (6603), 328-335 (2022)
   PUBMED   35857590
REFERENCE   5  (bases 1 to 13405)
  AUTHORS   Li,X., Zhu,M., Zhao,G., Zhou,A., Min,L., Liu,S., Zhang,N., Zhu,S.,
            Guo,Q., Zhang,S. and Li,P.
  TITLE     MiR-1298-5p level downregulation induced by Helicobacter pylori
            infection inhibits autophagy and promotes gastric cancer
            development by targeting MAP2K6
  JOURNAL   Cell Signal 93, 110286 (2022)
   PUBMED   35192930
  REMARK    GeneRIF: MiR-1298-5p level downregulation induced by Helicobacter
            pylori infection inhibits autophagy and promotes gastric cancer
            development by targeting MAP2K6.
REFERENCE   6  (bases 1 to 13405)
  AUTHORS   Moriguchi,T., Kuroyanagi,N., Yamaguchi,K., Gotoh,Y., Irie,K.,
            Kano,T., Shirakabe,K., Muro,Y., Shibuya,H., Matsumoto,K.,
            Nishida,E. and Hagiwara,M.
  TITLE     A novel kinase cascade mediated by mitogen-activated protein kinase
            kinase 6 and MKK3
  JOURNAL   J Biol Chem 271 (23), 13675-13679 (1996)
   PUBMED   8663074
REFERENCE   7  (bases 1 to 13405)
  AUTHORS   Stein,B., Brady,H., Yang,M.X., Young,D.B. and Barbosa,M.S.
  TITLE     Cloning and characterization of MEK6, a novel member of the
            mitogen-activated protein kinase kinase cascade
  JOURNAL   J Biol Chem 271 (19), 11427-11433 (1996)
   PUBMED   8626699
REFERENCE   8  (bases 1 to 13405)
  AUTHORS   Raingeaud,J., Whitmarsh,A.J., Barrett,T., Derijard,B. and
            Davis,R.J.
  TITLE     MKK3- and MKK6-regulated gene expression is mediated by the p38
            mitogen-activated protein kinase signal transduction pathway
  JOURNAL   Mol Cell Biol 16 (3), 1247-1255 (1996)
   PUBMED   8622669
REFERENCE   9  (bases 1 to 13405)
  AUTHORS   Han,J., Lee,J.D., Jiang,Y., Li,Z., Feng,L. and Ulevitch,R.J.
  TITLE     Characterization of the structure and function of a novel MAP
            kinase kinase (MKK6)
  JOURNAL   J Biol Chem 271 (6), 2886-2891 (1996)
   PUBMED   8621675
REFERENCE   10 (bases 1 to 13405)
  AUTHORS   Doza,Y.N., Cuenda,A., Thomas,G.M., Cohen,P. and Nebreda,A.R.
  TITLE     Activation of the MAP kinase homologue RK requires the
            phosphorylation of Thr-180 and Tyr-182 and both residues are
            phosphorylated in chemically stressed KB cells
  JOURNAL   FEBS Lett 364 (2), 223-228 (1995)
   PUBMED   7750576
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DB111443.1, BC012009.1,
            AA932919.1 and AC002546.2.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_002758.3.
            
            Summary: This gene encodes a member of the dual specificity protein
            kinase family, which functions as a mitogen-activated protein (MAP)
            kinase kinase. MAP kinases, also known as extracellular
            signal-regulated kinases (ERKs), act as an integration point for
            multiple biochemical signals. This protein phosphorylates and
            activates p38 MAP kinase in response to inflammatory cytokines or
            environmental stress. As an essential component of p38 MAP kinase
            mediated signal transduction pathway, this gene is involved in many
            cellular processes such as stress induced cell cycle arrest,
            transcription activation and apoptosis. [provided by RefSeq, Jul
            2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803612.290913.1, U39657.2
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1968540
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000590474.7/ ENSP00000468348.1
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-90                DB111443.1         1-90
            91-1553             BC012009.1         1-1463
            1554-1869           AA932919.1         11-326              c
            1870-13405          AC002546.2         82883-94418
FEATURES             Location/Qualifiers
     source          1..13405
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="17"
                     /map="17q24.3"
     gene            1..13405
                     /gene="MAP2K6"
                     /gene_synonym="MAPKK6; MEK6; MKK6; PRKMK6; SAPKK-3;
                     SAPKK3"
                     /note="mitogen-activated protein kinase kinase 6"
                     /db_xref="GeneID:5608"
                     /db_xref="HGNC:HGNC:6846"
                     /db_xref="MIM:601254"
     exon            1..304
                     /gene="MAP2K6"
                     /gene_synonym="MAPKK6; MEK6; MKK6; PRKMK6; SAPKK-3;
                     SAPKK3"
                     /inference="alignment:Splign:2.1.0"
     CDS             289..1293
                     /gene="MAP2K6"
                     /gene_synonym="MAPKK6; MEK6; MKK6; PRKMK6; SAPKK-3;
                     SAPKK3"
                     /EC_number="2.7.12.2"
                     /note="isoform 1 is encoded by transcript variant 1;
                     protein kinase, mitogen-activated, kinase 6 (MAP kinase
                     kinase 6); dual specificity mitogen-activated protein
                     kinase kinase 6; MEK 6; MAPKK 6; MAPK/ERK kinase 6; SAPK
                     kinase 3; stress-activated protein kinase kinase 3"
                     /codon_start=1
                     /product="dual specificity mitogen-activated protein
                     kinase kinase 6 isoform 1"
                     /protein_id="NP_002749.2"
                     /db_xref="CCDS:CCDS11686.1"
                     /db_xref="GeneID:5608"
                     /db_xref="HGNC:HGNC:6846"
                     /db_xref="MIM:601254"
                     /translation="MSQSKGKKRNPGLKIPKEAFEQPQTSSTPPRDLDSKACISIGNQ
                     NFEVKADDLEPIMELGRGAYGVVEKMRHVPSGQIMAVKRIRATVNSQEQKRLLMDLDI
                     SMRTVDCPFTVTFYGALFREGDVWICMELMDTSLDKFYKQVIDKGQTIPEDILGKIAV
                     SIVKALEHLHSKLSVIHRDVKPSNVLINALGQVKMCDFGISGYLVDSVAKTIDAGCKP
                     YMAPERINPELNQKGYSVKSDIWSLGITMIELAILRFPYDSWGTPFQQLKQVVEEPSP
                     QLPADKFSAEFVDFTSQCLKKNSKERPTYPELMQHPFFTLHESKGTDVASFVKLILGD
                     "
     exon            305..371
                     /gene="MAP2K6"
                     /gene_synonym="MAPKK6; MEK6; MKK6; PRKMK6; SAPKK-3;
                     SAPKK3"
                     /inference="alignment:Splign:2.1.0"
     exon            372..420
                     /gene="MAP2K6"
                     /gene_synonym="MAPKK6; MEK6; MKK6; PRKMK6; SAPKK-3;
                     SAPKK3"
                     /inference="alignment:Splign:2.1.0"
     exon            421..534
                     /gene="MAP2K6"
                     /gene_synonym="MAPKK6; MEK6; MKK6; PRKMK6; SAPKK-3;
                     SAPKK3"
                     /inference="alignment:Splign:2.1.0"
     exon            535..654
                     /gene="MAP2K6"
                     /gene_synonym="MAPKK6; MEK6; MKK6; PRKMK6; SAPKK-3;
                     SAPKK3"
                     /inference="alignment:Splign:2.1.0"
     exon            655..771
                     /gene="MAP2K6"
                     /gene_synonym="MAPKK6; MEK6; MKK6; PRKMK6; SAPKK-3;
                     SAPKK3"
                     /inference="alignment:Splign:2.1.0"
     exon            772..823
                     /gene="MAP2K6"
                     /gene_synonym="MAPKK6; MEK6; MKK6; PRKMK6; SAPKK-3;
                     SAPKK3"
                     /inference="alignment:Splign:2.1.0"
     exon            824..951
                     /gene="MAP2K6"
                     /gene_synonym="MAPKK6; MEK6; MKK6; PRKMK6; SAPKK-3;
                     SAPKK3"
                     /inference="alignment:Splign:2.1.0"
     exon            952..1029
                     /gene="MAP2K6"
                     /gene_synonym="MAPKK6; MEK6; MKK6; PRKMK6; SAPKK-3;
                     SAPKK3"
                     /inference="alignment:Splign:2.1.0"
     exon            1030..1169
                     /gene="MAP2K6"
                     /gene_synonym="MAPKK6; MEK6; MKK6; PRKMK6; SAPKK-3;
                     SAPKK3"
                     /inference="alignment:Splign:2.1.0"
     exon            1170..1215
                     /gene="MAP2K6"
                     /gene_synonym="MAPKK6; MEK6; MKK6; PRKMK6; SAPKK-3;
                     SAPKK3"
                     /inference="alignment:Splign:2.1.0"
     exon            1216..13405
                     /gene="MAP2K6"
                     /gene_synonym="MAPKK6; MEK6; MKK6; PRKMK6; SAPKK-3;
                     SAPKK3"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1531..1536
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MAP2K6"
                     /gene_synonym="MAPKK6; MEK6; MKK6; PRKMK6; SAPKK-3;
                     SAPKK3"
                     /note="hexamer: ATTAAA"
     polyA_site      1558
                     /gene="MAP2K6"
                     /gene_synonym="MAPKK6; MEK6; MKK6; PRKMK6; SAPKK-3;
                     SAPKK3"
                     /note="major polyA site"
     regulatory      1850..1855
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MAP2K6"
                     /gene_synonym="MAPKK6; MEK6; MKK6; PRKMK6; SAPKK-3;
                     SAPKK3"
                     /note="hexamer: TATAAA"
     polyA_site      1869
                     /gene="MAP2K6"
                     /gene_synonym="MAPKK6; MEK6; MKK6; PRKMK6; SAPKK-3;
                     SAPKK3"
                     /note="major polyA site"
     regulatory      11503..11508
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MAP2K6"
                     /gene_synonym="MAPKK6; MEK6; MKK6; PRKMK6; SAPKK-3;
                     SAPKK3"
                     /note="hexamer: AATAAA"
     polyA_site      11525
                     /gene="MAP2K6"
                     /gene_synonym="MAPKK6; MEK6; MKK6; PRKMK6; SAPKK-3;
                     SAPKK3"
                     /note="major polyA site"
     regulatory      13381..13386
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MAP2K6"
                     /gene_synonym="MAPKK6; MEK6; MKK6; PRKMK6; SAPKK-3;
                     SAPKK3"
                     /note="hexamer: AATAAA"
     polyA_site      13405
                     /gene="MAP2K6"
                     /gene_synonym="MAPKK6; MEK6; MKK6; PRKMK6; SAPKK-3;
                     SAPKK3"
                     /note="major polyA site"
ORIGIN      
        1 agttccaagt ttggagcttt tagctgccag ccctggccca tcatgtagct gcagcacagc
       61 cttccctaac gttgcaactg ggggaaaaat cactttccag tctgttttgc aaggtgtgca
      121 tttccatctt gattccctga aagtccatct gctgcatcgg tcaagagaaa ctccacttgc
      181 atgaagattg cacgcctgca gcttgcatct ttgttgcaaa actagctaca gaagagaagc
      241 aaggcaaagt cttttgtgct cccctccccc atcaaaggaa aggggaaaat gtctcagtcg
      301 aaaggcaaga agcgaaaccc tggccttaaa attccaaaag aagcatttga acaacctcag
      361 accagttcca caccacctcg agatttagac tccaaggctt gcatttctat tggaaatcag
      421 aactttgagg tgaaggcaga tgacctggag cctataatgg aactgggacg aggtgcgtac
      481 ggggtggtgg agaagatgcg gcacgtgccc agcgggcaga tcatggcagt gaagcggatc
      541 cgagccacag taaatagcca ggaacagaaa cggctactga tggatttgga tatttccatg
      601 aggacggtgg actgtccatt cactgtcacc ttttatggcg cactgtttcg ggagggtgat
      661 gtgtggatct gcatggagct catggataca tcactagata aattctacaa acaagttatt
      721 gataaaggcc agacaattcc agaggacatc ttagggaaaa tagcagtttc tattgtaaaa
      781 gcattagaac atttacatag taagctgtct gtcattcaca gagacgtcaa gccttctaat
      841 gtactcatca atgctctcgg tcaagtgaag atgtgcgatt ttggaatcag tggctacttg
      901 gtggactctg ttgctaaaac aattgatgca ggttgcaaac catacatggc ccctgaaaga
      961 ataaacccag agctcaacca gaagggatac agtgtgaagt ctgacatttg gagtctgggc
     1021 atcacgatga ttgagttggc catccttcga tttccctatg attcatgggg aactccattt
     1081 cagcagctca aacaggtggt agaggagcca tcgccacaac tcccagcaga caagttctct
     1141 gcagagtttg ttgactttac ctcacagtgc ttaaagaaga attccaaaga acggcctaca
     1201 tacccagagc taatgcaaca tccatttttc accctacatg aatccaaagg aacagatgtg
     1261 gcatcttttg taaaactgat tcttggagac taaaaagcag tggacttaat cggttgaccc
     1321 tactgtggat tggtgggttt cggggtgaag caagttcact acagcatcaa tagaaagtca
     1381 tctttgagat aatttaaccc tgcctctcag agggttttct ctcccaattt tctttttact
     1441 ccccctctta agggggcctt ggaatctata gtatagaatg aactgtctag atggatgaat
     1501 tatgataaag gcttaggact tcaaaaggtg attaaatatt taatgatgtg tcatatgagt
     1561 cctcaagctt ctcagacttc tcttattctt tacaaaatga atgcattggc cctgacaaaa
     1621 aggtgctacg gtagtgatga aattataagt agatttgtag tttgtcccat ttattatttt
     1681 aatatttatg tttaagtgct tggttgaaaa gattccattt tatacaagaa gggagattca
     1741 aaaaaaaaat ataaggttgg gttagcaata tttatagggc ttttattttt taagttcaat
     1801 tgtgtctgtg gtccagaaga aattatttaa tatgcatctt tgagaatatt ataaaaatat
     1861 caaaaaggag ctcttcttgt gaaatgtctg ttccagctgt tgtgactgct gccatttttg
     1921 caaacatctg cccaatcctg ggtgatcacc acatctttta ggggaagtga caagatgctc
     1981 tggtcatact ctttttccca actttggaaa acataaaaat cactcatata acagctcaaa
     2041 gagtaaaaca tttggttctt ctgacacttg tggtatagta ttagtggaaa gtgatttgta
     2101 atatgatttt atatccacct acctattcat ctacctgtgt gtatgtgtgt gtttgtgtgt
     2161 ctatttggca attcacaagt cctgccaagt ggtttctatg agcatctctg tttggtaagg
     2221 aggacaattg tcagttttga gggggacatg tgttaaatca cagaaaaaaa tggtgccttc
     2281 ttctgcgttt gtccctcctg ccatgtgtaa gttgtaagga ttgcctttgt agttaatgta
     2341 ctctttggct ttgtttgttt gttttcttct tcagtgaagc agccttacta ttcatagaag
     2401 ggctagaata ggagaaaatg aaaggtagtg agtaattctt tgataagatg aggaaataat
     2461 gggaaaggtt gaattaattc ctgggcatgg actaccagat gaccacaagt tgcgttgagg
     2521 ccgcatcttt cttcagcagc gtgcaatagc tggctcctct ataggagatg agcttcattg
     2581 ggagttccta gcaagttgac taaacagcaa aagttctttc tcgtgggtaa atatacccac
     2641 aggttctatg atttgtagct ctaggtttct tgatgatcaa ggagtgaagt aattgacagg
     2701 gaaaatatag acctatgata aataaccagg aagcattgct tttggacaag gaaggacaga
     2761 gggttttgat tttaaaaaga agaaaaaaaa accttatttt ttctttcttg gcctcaagtt
     2821 caatatggag aggattgctt ccctgaatcc tctcttcctt ccccttttag attttgaagt
     2881 gcaatcatat gtttttctct gtttgcattt tttcctcctt gttcttgaca aggaggagtt
     2941 gctcctgccc agaatgagcg tgacacttcc gaacacttct tcatattcag ttccaagata
     3001 tatctgcttg attaaacatg agcttcctct gctctgaagc tacctctgtc ctcattttat
     3061 tctagccaga aaaggagtat caccctagtg attatggctg ttcactttcc catctatctt
     3121 cctaaatctg gaagttcttc tcttggagat caagagaaaa attacaattg tattccttac
     3181 tttattcacc cacctatgaa aacaggaagc aataggaaaa aaaatccggt tactccattt
     3241 tagcttttgg tgaacgatgt agagcaaatt gtctctctgg tctaggtccg attactctta
     3301 cctgtttttc cactttgaga cattctaaac agaatgtgta acttctcata tgtatgcctc
     3361 tcccatctgt gaacctaggc caaagttgca aaaacaatca tattaatagt agagtagaga
     3421 aaaagttagt ctatggttct caaccctcgt gtacattgga accatctggg agctgtgaac
     3481 actgtcgctg ccaatgttcc agccaccaga gatttggatt taactggtgg ggcctaggaa
     3541 ttggtgtttt tttgttttgt tttgtttttt acacaccttc atgtgattct gatgtgaagc
     3601 tgggttcaga acacttatct agtaccttct aaagagaact gacttaaatt tactttcttt
     3661 tgaacatttg cagggagtaa catgccattg cagaaagtaa caaaaacagg tcctatttct
     3721 ttccctgtcc tcatcagtgg aaatctcttt gtcactctga gagaaggcat gtacctggga
     3781 tactgatagg aagtgtagaa caccttttcc ccagagaagc aatattttgc actgttatta
     3841 aatatcttac acggtaaagt caaaagaatg acctgatagc ctcacaagac taaattttag
     3901 agcatggttt tgtttttgga aaactgtgtt gtaagtgcca atcaaccaac ttttgaaaaa
     3961 atcaagatac ctaaactata tataaatggg gagtattctg tacatataga cttatatata
     4021 aagacatctg tgttcacgga tgaccctcaa aatagttaat gccccaccag catagaccat
     4081 ctgaatatca gccctgtcta cacctatcaa tgtattacaa aatcagtata gctctacaaa
     4141 agagatcatg cttatttccc cagatgtatt tgattttgta tcatataatt gtccatgtta
     4201 taatttttga aaatgtttat taaaatagcc atcttttttg atattattgg tttaagaggt
     4261 gtgccaaaaa aagtaatatg cataactttt aagactatta ccctatgttt gtacgtatga
     4321 gtgaatattg cccaccagag tagccatctt gagagactac atatttatat tcataatgct
     4381 attaaattat ttttgccact cctctttcag aaaaggcttt agaatccact ccctcctctg
     4441 agatgtgtgt catcatttga gaattcttac ttaggttttg ttgttgtttg ttttgctttt
     4501 tacaaaaatc cttagcagat gtttccctct ttgatttacc tgccttgttt atcagatttt
     4561 gcacaaagtt gtgtttgaca atttctagaa gttaaatctt ccctcagagc tggagtttta
     4621 gcatcattga ctctttgtaa aacgccatgt catgggctct gaagataatt tcaaatgaag
     4681 atttccacac cccgccccca ccacccctgc ccaaagtgca tgattatttt taaccagagt
     4741 cattcttcca ccagaataag tgtaatctcc caaaatgact actttgaagg agatagaacc
     4801 cccataaagg tatatgtttg ttgataaaat atcaggtcat cacggatttt gcaagtgaaa
     4861 gtcacctatc ttctatgatt gaaggtcctg atgtggggga ataatctatt ttttctaaag
     4921 actgtgtttg gtcacactga tttaatcaga acaaatgggt taaataagca gcttttatca
     4981 cagttaagcc atctgaaatg gaaacgagta tgtatgggca tggcttgaaa ttgtttgtat
     5041 tttacagttc ttgtatatcc ttcaagccta acaaaaaatt gtatgtgcca gagattccta
     5101 aactttctgt gttcagggtg ccattagttg tcttggtact tttttcatgg tgccccaggt
     5161 caaaatatat acttaatagt tcagtgtttt aagtaattag gtcccaacag cttaataata
     5221 gcagtttgca cagtgtcctg catatatcac tatatttccc ttaaaaattt ccagcattgg
     5281 ctgagcatgg tgactcacgc ctgtaatccc agcactttgg gaggctgagg caggtggatc
     5341 acctcaggtc aggaatttga gaccagcctg actaacatga tgaaaccccg tctctactaa
     5401 aaatacaaaa ttagctgggc atggtggcac atgcctgtaa tcccagctac tcaggaggct
     5461 gaggcaggag aattgcttga acccgggagg cggaggttgc agtgagccga catcgtgtca
     5521 gtgcactcca gcctgggcaa caagagcaaa acttcatctc aaaaaaaaaa aaaaaaaatg
     5581 tacaacatct tgcttagcct gtgtgtgttc tgtggtacct tggaatagct cagtacataa
     5641 tttggggacc acagatatct attatgccaa tgtttgcact gtctgctgtt ggtgaccctc
     5701 aaaatgaaat tcttggccac tcaactctcc acattatttc actctttgtt ttgttattat
     5761 tagtattctt cttgcttgcc attggctaat tcatttttta actgcaaccc tactcttctt
     5821 tgctgttcat cagccctgca atgccgtagt gtcttagtct agaaaaatgc aatactcaaa
     5881 agccccagca ttggaggagg ctggtgctaa gatggacagg gttcctgatt tctctcattg
     5941 aacttgattt agtgtcttgg gaattataat ctcaaaggag gcagagaggg gttaatgttg
     6001 cataatttat cactaaaatg tctttgttga ccaaggggct ttattaatta tgctcagaga
     6061 aacaattctg tttctcttaa aagtgtctaa caaaacactt ttttctttgg cctgaaagga
     6121 caatggatac ctagttccta atttcctacc caaatgctgt tttggctgtg ttactccctc
     6181 tgccctcgaa gctaagattt atatatttac aaaatttatt ggagctggta gtcagatcta
     6241 gtaaaatgga ttaaatgtca attgtgctgg gattttgcct taacatctat ctatgacttg
     6301 aagagggatt tgttggctca aaggatcttc tgcttttaat gaattagcaa gtgaaaaggt
     6361 atttgaataa atgtcaactt cataggactt tttttttttt taacttttca aatggaaggt
     6421 gcagttttca attaggcctc tgaaaattta catagtcaga tggaaaaatg ccagagtaaa
     6481 atctaggaag aaggagctac cagacccgat agaatagaaa gaaagctatt ttattctcgg
     6541 aggtctatgt tcctcttgtg tttgagtgcc tctagcactg ttaaatgtgc tgacagctaa
     6601 agatgctctt tgggtttttt ttttggcttt aatttgggta ctatggattc ttttgggaat
     6661 ttgttgaaag ctaggacctt ttccccagga aaatttacat tgtatatgaa gcacataatt
     6721 ttgcaaactt tttttggttg ttgttgggag ggggttgcag tttatatact ccacggggtt
     6781 agtagctctg ttctgtagaa tattgactgt gacatcctag accacggttg gcaaactttt
     6841 tctgtatagg accagatagt aaatattttt ggctttgtgt gttataccat ctctgtcaca
     6901 gttactcagc tctgctgttg tatcatgaaa gcatctatac gcaatacaca aatgaatgag
     6961 cctggtggct gtgttccaat aaaactttat ttacaaacca agcaatgggc caaatttggc
     7021 ccacaggcca tggtttgcca acaccagtct tagagcatta aatataaaac tctgattaac
     7081 tagatatgta gagttcttcc attttagtga ctattgagct cagctgctgt tgaggcagat
     7141 taggaagatg gacataggaa actggattca gaaaggatga ggactgttta gtcccatgaa
     7201 agttgcttgt taatgtcctc aggtaagtat gaattgttct ggaagctgat agaacaattt
     7261 tcttcagatc aaactgaagt acttactttt tccatttcta tgcaatcacc aacataattt
     7321 acttcaattt ggaaataaat gtcacagttc tcttagttgt taactgtatc cttggcttag
     7381 gttatttgca ttttctttct ttctttctgt agtgtggttt atacacaagg agaatcacga
     7441 acccagacac tagtcaatct ctctattccc tgacttgtac tgagattggg gaatttggga
     7501 ggtcagactt acctcaaacg tagaagaagg cagatagagt tcttaacctt tttcaactta
     7561 gccacctcaa ttatttgttc acattttaag gaagtaggaa agagtagttt gaagtcacaa
     7621 aatttgttct caggtgttct taaagctccc tgttctcact gcgacagaag actcaggcct
     7681 actcattttg tgctgtccca caaaagtgag aggagtactt ctcttttttt aaatcatcag
     7741 taaatttcaa ttttaagggg cctatgcaaa atgcctcctt tctgatgtga ttttcttggg
     7801 ttgctggccc tagttgaatt tatgggccct gaagcctcta gtggaaatct tgtcttccct
     7861 atagaacgag aacagctatg taatttgctt caccttctgt taggacttgc accctctttg
     7921 ccatacagaa tgctataaaa aggacagtct gccagtgacc gaagctttct catttttttt
     7981 tcttccagaa caatagcaca catcttggtt aaagctatag tctccttatt attcagaaat
     8041 attctttttc ctgctgcacc attaggcaaa catacattat gcttagaatg atacttggaa
     8101 actccttaac agggcatatt gaagtatttg atccagcaac ttacctaaaa gaatgtttgc
     8161 tcttcaccta gggaaataaa acctgaattt cagagccttc aaaatgaaat tatccttcca
     8221 ggggaagcac attgccacca aatacatcac tcactacctg ttcctggtga ctacatagaa
     8281 gatgtgttat ttttctgagg tttagaaagt cactgtttac agctatgcaa atattgtact
     8341 attacagatt tttctaatga agtagtttga aatcaaggct ttagtggaag gtaatctttt
     8401 cagtttctga cccagatttc tttttcaagc aaaactcctc tgaaagcctc tttgctatag
     8461 aggtgatgaa ggcacttgct agcctaagca gaaacataaa gtaaaaaatt ttgtagtagg
     8521 gaatttttgt tggtaagaaa tcagtatcat cttgtaacac aaacacgtgt taatagaact
     8581 taaaaatact cagcctaatt ccttgggact ttcagtatct tgacatcact tgtattatca
     8641 tttgaacttg gacattgagc cctttatttt tgggagttta cagttaaatt ttggaagaat
     8701 tgtgttgtat ttctttctta gatgttgtca gtatgaacag aatttttttg tgaacagtta
     8761 atcttgatgt gctccatagc tttctccagt ttacactttt gcatttctga gattcagggt
     8821 ctttttcaag gaaggaggct aatgtttaag gcctggaggc tgaattcagg gagcgtattg
     8881 gcaagtttag gcacttactt gtgtcttaat gtgggaaaca gaactttcta agtaatctct
     8941 ggagtttgta gcttagacca ggccttcaaa agtcttttct gttttccttt gctacaattt
     9001 gcttgttatt tctctgccgg tcacagatga cctggactga ctgaatgctt ttgtggtaag
     9061 gaactgatct ggccattttc atataacaaa aatcaaagtc aacaattttg tatcaggctg
     9121 cctaaatgaa ccctattgtt tccagttctt aaaaatttaa gggctatcta agaaaagttt
     9181 aagcaaaacc ctcattccaa acatgcgacc ttataataag aacttccttt aaagatgagc
     9241 agcaaggttg ggtatctgat ttcactaagt aatattctat tgtggtcaga aatgggtaat
     9301 ttgcatcatt tggtcactat caatatttgt gttggagtct gcaagatatt tcaacaaagt
     9361 aagccaaacc actatcttag gggattgttg ctggactttg gaatataagg ctgaacagtg
     9421 atgtgaagtc atgtttgggg gctggaagaa gtgataaatg caaaggttgg tgctaaatta
     9481 ggaacccctt gaaggagcaa gctgattaaa aaaaaaagct ggcagacaag tatatctttt
     9541 aatttatttg cagtgttgct atattataga gatgatttcc tatgggaaaa cccatcaaaa
     9601 agccaaacct ttattgttat ttttccttaa aaatactgag ctataagaag attcagagag
     9661 tggcattaat ttgggcatca gaacattttc ttttgtatcc ctagtgttat tgatttgaaa
     9721 gagttacctt ttcagacaga tggctgaaca aaagtaaatg attaacggga aatttgatgg
     9781 ttgagaaaaa ggaacgatat gcctaaagca ttttgagaat atacccctca tccatcagcc
     9841 acctctgggt aaagaaacac aaataccaaa gcctgagctc cttaaccttt tgttccagag
     9901 ggcagacatt tttaagaaag gtgaatgtta gagaaggtta cctgatgagc aagcttcttt
     9961 cccataattc agagaactgt gaatgtactt agaaatacac tacaggtctt caccagatga
    10021 actagatttt ataattttaa aatataatac tgaaagctag tttgaagttt cagaagccat
    10081 gaattatggg gaaggagtag ttttttattt tattttattt ttctgatctc aagttgtttg
    10141 tcctgttgta tgttcaatac ttggggagat aagagcgagg tacagctgtg gttttcagac
    10201 catattcagt ggtgcccctg agggtctctt gtgaacagaa gggaaaaaga gtgtgatgaa
    10261 ggtgaactct gcctatctga acctctgtca acctccagtc agaatatctg gcttctagta
    10321 ttgttccttt taactggaag tctctgtggc cattaaaaac ttgggaacgt tggattaaat
    10381 gaccacttta ggactttaaa cagtctcaaa tatgggaaat tttatagcca accacggctg
    10441 tgagtccctg gcttttgccg tactgagtat gctcacagag atagggagat aggtggccag
    10501 aagacagggt catttaattt taattgagca taaatcattt tgaaagaaaa atgcaaggaa
    10561 ttgttgtatg acagccatgc attatagatc cttacatgcg acattttcct aaagtggttg
    10621 agaatgacct gatctttgtt caccgtctca gtgacaaggc gtggagtgac tgggctcttc
    10681 atatgcagtg gaatttttgc atctctaggt ttgcagaggc aggagttacc gtttttgttc
    10741 attgacctat cagaaaaaag caaatccttt ggacaatgtt gacacagaca ggggtacggt
    10801 ctgagactca agctaacaga gctacccctt gctgcctttt gcaaaggtgt tgtaggtgga
    10861 gaagggtaat ggaaacctgg tacagccttt agaagttgga agctatggtg gtgtatctgt
    10921 catgaactgc acacaaggga atgcttaaac accagctgag tcatatcagg tgccttgtac
    10981 acacacataa agagtctggt gaattctgac agtgttctgt ttgccactag agcaaattta
    11041 atagctgggg tttcacagca actgttttag aaaactatat gtgccaaaaa tttacattgg
    11101 gcagcagttt atagtgttct tggccaatct gcataaaagc cacttgagga ggtttgatta
    11161 agaaaattgt gtttatctcc tgtattacct ctgtgtttga tttattcttt agtctcaaat
    11221 ttattttctg agtggactga ttttctatat gaactgaaat gatgttttaa tagaataata
    11281 ggtattttta gaggaaaagt atttttttgt gtaatttgct tacacaacta ggacatactt
    11341 cctatgatac tgaatcatca aattgagtca tttaaagctg aaagaggtgt taggaatgta
    11401 gtttcacatt atttaaatac atgaacagtt ttctatatat tttgtgaaaa tcttgatgag
    11461 acactagaat ttctttatgg aattgaactt tacaagaatt ttaataaaag aggtggattt
    11521 cttcagcttt ctttgtgctt cagtttcata gctgaaaatg ctgcttccgt ttattaatat
    11581 ggactttgta aggaaacaca acaacacgtt ttcttacctt ctgtaaattt tgtgatagac
    11641 acatgttatt tgtatatatg attgattgtt tgcctgttgc accctaaagt tattttcaaa
    11701 ccatgtttat tgcaaagaga gcctttgggc aagtggaaaa tgccctgatg ctagaatgag
    11761 gtagttccat aagctagtta ggagcttgct acctcttctt ggtacctgaa atattctgaa
    11821 aggatatcgg agaggtccta tgcacccctg tctttcaaaa cccacctcca gcacttcaaa
    11881 gtagtgtctc tggagagttt aaaataaaag aatgaatgct attcagtgga ttccctcatt
    11941 gaggctccca tctttcctgc caggtgcagc tttttctggt tggaatcatc tcttctttac
    12001 ggattgccgc attgtctctt tgtgaatgag gcaggctgaa ctgtagagca tgaaactcat
    12061 tagaagttta taaagtaaag acctgtaaag catgtgggtg gaatgtttcc atgctcttga
    12121 ggtgaatatt aaatttaaat tctggccttt gggaactctt tgcttgtgag ctgaagaagg
    12181 aaagaaggag ttgggggtgt atatctaact gtgtttttct atatggaaat atatgagcat
    12241 caagtgataa cttcaataag gcctcaggat tgtatttaaa atacctgttt tgtgggacag
    12301 catgcctttg ttttctttgc ctgttggctt tggtggctcc aaacattttc attttaggct
    12361 agctttcctg tcacccaggt tgtgtgcatt ttttttttca tttgaactat tgtttatcat
    12421 tattaatgat gttatctcca aatcccaaag ccaaggaaat agccagtatg caggacttgc
    12481 agtagatata agcattggtg ttaacatagg ttaagttttg ttagtgttcc cagaaatata
    12541 ctgaattgag ggataatgta gctttaaaga aattatgttt ctttttaaca tttggagaag
    12601 ccacctgtcc tgggtcccta ttcttgagaa attcatcttt tcatgcaaat aacattgatg
    12661 ggggacaaga ctggatgatt gacttctatc agtcagtaga caaggaagta taataattgc
    12721 caaaggtgag ggtaattttg ccttacaagt atgtaggtca ttctgtggtg ggatttccca
    12781 tcacatctag taaaaaacaa ccttttcatt tccctccttt ctaatccaag atcatatttt
    12841 taaaaagtag gtttctgatg tgccatgaaa tatttctgtg aatctgtgtt tttgaccaag
    12901 gaaacagctg agatattaaa ccatgtggtt gttccacggt tcatctggct accgttctgg
    12961 gtcccctctg accacctcaa aaagaaaatg aaattgggag attaaatcaa gcttgacctc
    13021 ctcttttaat gaggaacttt cacgttgact tcctatctca ggatattctt cagtttcata
    13081 ctgctgagga gaaaggaaca agctgcagac actgtaactg gtctccagat gtgtgtatat
    13141 gcgtgtaaaa cttcacaccg tgtgtgttgt gttcaatgtt gtgtcaatct acaaactgac
    13201 tcaaacaaca gtttaacgat agagaagaca gtgataatgg caaaaaaaac acccaaccac
    13261 ctttttccgt caaagtgctt gctatggctt tcatagctgg gacaagtaac attaagtatt
    13321 caggagcaaa gtgttcttga aagaaaatgg tgtgttgatc tcataagaaa atgtacaacc
    13381 aataaaagac attttaaaaa gcgta
//
