LOCUS       NM_001375505            9650 bp    mRNA    linear   PRI 12-OCT-2024
DEFINITION  Homo sapiens microtubule associated protein 2 (MAP2), transcript
            variant 67, mRNA.
ACCESSION   NM_001375505 XM_024452900
VERSION     NM_001375505.1
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 9650)
  AUTHORS   DeGiosio,R.A., Needham,P.G., Andrews,O.A., Tristan,H.,
            Grubisha,M.J., Brodsky,J.L., Camacho,C. and Sweet,R.A.
  TITLE     Differential regulation of MAP2 by phosphorylation events in
            proline-rich versus C-terminal domains
  JOURNAL   FASEB J 37 (10), e23194 (2023)
   PUBMED   37702880
  REMARK    GeneRIF: Differential regulation of MAP2 by phosphorylation events
            in proline-rich versus C-terminal domains.
REFERENCE   2  (bases 1 to 9650)
  AUTHORS   Holden,M.R., Krzesinski,B.J., Weismiller,H.A., Shady,J.R. and
            Margittai,M.
  TITLE     MAP2 caps tau fibrils and inhibits aggregation
  JOURNAL   J Biol Chem 299 (7), 104891 (2023)
   PUBMED   37286038
  REMARK    GeneRIF: MAP2 caps tau fibrils and inhibits aggregation.
REFERENCE   3  (bases 1 to 9650)
  AUTHORS   Nishida,K., Matsumura,K., Tamura,M., Nakamichi,T., Shimamori,K.,
            Kuragano,M., Kabir,A.M.R., Kakugo,A., Kotani,S., Nishishita,N. and
            Tokuraku,K.
  TITLE     Effects of three microtubule-associated proteins (MAP2, MAP4, and
            Tau) on microtubules' physical properties and neurite morphology
  JOURNAL   Sci Rep 13 (1), 8870 (2023)
   PUBMED   37258650
  REMARK    GeneRIF: Effects of three microtubule-associated proteins (MAP2,
            MAP4, and Tau) on microtubules' physical properties and neurite
            morphology.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 9650)
  AUTHORS   Plucarova,J., Jansen,S., Narasimhan,S., Lanikova,A., Lewitzky,M.,
            Feller,S.M. and Zidek,L.
  TITLE     Specific phosphorylation of microtubule-associated protein 2c by
            extracellular signal-regulated kinase reduces interactions at its
            Pro-rich regions
  JOURNAL   J Biol Chem 298 (10), 102384 (2022)
   PUBMED   35987383
  REMARK    GeneRIF: Specific phosphorylation of microtubule-associated protein
            2c by extracellular signal-regulated kinase reduces interactions at
            its Pro-rich regions.
REFERENCE   5  (bases 1 to 9650)
  AUTHORS   Svingos,A.M., Robicsek,S.A., Hayes,R.L., Wang,K.K., Robertson,C.S.,
            Brophy,G.M., Papa,L., Gabrielli,A., Hannay,H.J., Bauer,R.M. and
            Heaton,S.C.
  TITLE     Predicting Clinical Outcomes 7-10 Years after Severe Traumatic
            Brain Injury: Exploring the Prognostic Utility of the IMPACT Lab
            Model and Cerebrospinal Fluid UCH-L1 and MAP-2
  JOURNAL   Neurocrit Care 37 (1), 172-183 (2022)
   PUBMED   35229233
  REMARK    GeneRIF: Predicting Clinical Outcomes 7-10 Years after Severe
            Traumatic Brain Injury: Exploring the Prognostic Utility of the
            IMPACT Lab Model and Cerebrospinal Fluid UCH-L1 and MAP-2.
REFERENCE   6  (bases 1 to 9650)
  AUTHORS   Alberts,M.J., Kandt,R.S., Pericak-Vance,M.A., Bebout,J.,
            Speer,M.C., Siddique,T.S., Yamaoka,L., Hung,W.Y., Gaskell,P.C. and
            Roses,A.D.
  TITLE     MspI RFLP for microtubule associated protein-2 (MAP2)
  JOURNAL   Nucleic Acids Res 19 (4), 960 (1991)
   PUBMED   1708129
REFERENCE   7  (bases 1 to 9650)
  AUTHORS   Dammerman,M., Yen,S.H. and Shafit-Zagardo,B.
  TITLE     Sequence of a human MAP-2 region sharing epitopes with Alzheimer
            neurofibrillary tangles
  JOURNAL   J Neurosci Res 24 (4), 487-495 (1989)
   PUBMED   2481044
REFERENCE   8  (bases 1 to 9650)
  AUTHORS   Rubino,H.M., Dammerman,M., Shafit-Zagardo,B. and Erlichman,J.
  TITLE     Localization and characterization of the binding site for the
            regulatory subunit of type II cAMP-dependent protein kinase on MAP2
  JOURNAL   Neuron 3 (5), 631-638 (1989)
   PUBMED   2701845
REFERENCE   9  (bases 1 to 9650)
  AUTHORS   Obar,R.A., Dingus,J., Bayley,H. and Vallee,R.B.
  TITLE     The RII subunit of cAMP-dependent protein kinase binds to a common
            amino-terminal domain in microtubule-associated proteins 2A, 2B,
            and 2C
  JOURNAL   Neuron 3 (5), 639-645 (1989)
   PUBMED   2561973
REFERENCE   10 (bases 1 to 9650)
  AUTHORS   Kosik,K.S., Orecchio,L.D., Bakalis,S., Duffy,L. and Neve,R.L.
  TITLE     Partial sequence of MAP2 in the region of a shared epitope with
            Alzheimer neurofibrillary tangles
  JOURNAL   J Neurochem 51 (2), 587-598 (1988)
   PUBMED   2455776
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC019106.3, AC108072.3,
            AC079833.4 and AC006385.3.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Oct 29, 2019 this sequence version replaced XM_024452900.1.
            
            Summary: This gene encodes a protein that belongs to the
            microtubule-associated protein family. The proteins of this family
            are thought to be involved in microtubule assembly, which is an
            essential step in neurogenesis. The products of similar genes in
            rat and mouse are neuron-specific cytoskeletal proteins that are
            enriched in dentrites, implicating a role in determining and
            stabilizing dentritic shape during neuron development. A number of
            alternatively spliced variants encoding distinct isoforms have been
            described. [provided by RefSeq, Jan 2010].
            
            Transcript Variant: This variant (67), as well as variants 1, 9,
            and 10, encodes isoform 1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR11853559.19892.1,
                                           SRR14372079.1691503.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968540, SAMEA1968968
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000682079.1/ ENSP00000507035.1
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-230               AC019106.3         45345-45574
            231-280             AC019106.3         128890-128939
            281-345             AC108072.3         4794-4858
            346-422             AC079833.4         15024-15100
            423-713             AC079833.4         43113-43403
            714-827             AC079833.4         68543-68656
            828-905             AC079833.4         70721-70798
            906-4631            AC006385.3         9532-13257
            4632-4838           AC006385.3         13449-13655
            4839-4973           AC006385.3         13824-13958
            4974-5035           AC006385.3         17184-17245
            5036-5183           AC006385.3         22487-22634
            5184-5524           AC006385.3         26821-27161
            5525-5606           AC006385.3         42616-42697
            5607-5719           AC006385.3         46757-46869
            5720-9650           AC006385.3         47089-51019
FEATURES             Location/Qualifiers
     source          1..9650
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="2"
                     /map="2q34"
     gene            1..9650
                     /gene="MAP2"
                     /gene_synonym="MAP-2; MAP2A; MAP2B; MAP2C"
                     /note="microtubule associated protein 2"
                     /db_xref="GeneID:4133"
                     /db_xref="HGNC:HGNC:6839"
                     /db_xref="MIM:157130"
     exon            1..230
                     /gene="MAP2"
                     /gene_synonym="MAP-2; MAP2A; MAP2B; MAP2C"
                     /inference="alignment:Splign:2.1.0"
     exon            231..280
                     /gene="MAP2"
                     /gene_synonym="MAP-2; MAP2A; MAP2B; MAP2C"
                     /inference="alignment:Splign:2.1.0"
     exon            281..345
                     /gene="MAP2"
                     /gene_synonym="MAP-2; MAP2A; MAP2B; MAP2C"
                     /inference="alignment:Splign:2.1.0"
     exon            346..422
                     /gene="MAP2"
                     /gene_synonym="MAP-2; MAP2A; MAP2B; MAP2C"
                     /inference="alignment:Splign:2.1.0"
     exon            423..713
                     /gene="MAP2"
                     /gene_synonym="MAP-2; MAP2A; MAP2B; MAP2C"
                     /inference="alignment:Splign:2.1.0"
     CDS             452..5935
                     /gene="MAP2"
                     /gene_synonym="MAP-2; MAP2A; MAP2B; MAP2C"
                     /note="isoform 1 is encoded by transcript variant 67"
                     /codon_start=1
                     /product="microtubule-associated protein 2 isoform 1"
                     /protein_id="NP_001362434.1"
                     /db_xref="CCDS:CCDS2384.1"
                     /db_xref="GeneID:4133"
                     /db_xref="HGNC:HGNC:6839"
                     /db_xref="MIM:157130"
                     /translation="MADERKDEAKAPHWTSAPLTEASAHSHPPEIKDQGGAGEGLVRS
                     ANGFPYREDEEGAFGEHGSQGTYSNTKENGINGELTSADRETAEEVSARIVQVVTAEA
                     VAVLKGEQEKEAQHKDQTAALPLAAEETANLPPSPPPSPASEQTVTVEEDLLTASKME
                     FHDQQELTPSTAEPSDQKEKESEKQSKPGEDLKHAALVSQPETTKTYPDKKDMQGTEE
                     EKAPLALFGHTLVASLEDMKQKTEPSLVVPGIDLPKEPPTPKEQKDWFIEMPTEAKKD
                     EWGLVAPISPGPLTPMREKDVFDDIPKWEGKQFDSPMPSPFQGGSFTLPLDVMKNEIV
                     TETSPFAPAFLQPDDKKSLQQTSGPATAKDSFKIEEPHEAKPDKMAEAPPSEAMTLPK
                     DAHIPVVEEHVMGKVLEEEKEAINQETVQQRDTFTPSGQEPILTEKETELKLEEKTTI
                     SDKEAVPKESKPPKPADEEIGIIQTSTEHTFSEQKDQEPTTDMLKQDSFPVSLEQAVT
                     DSAMTSKTLEKAMTEPSALIEKSSIQELFEMRVDDKDKIEGVGAATSAELDMPFYEDK
                     SGMSKYFETSALKEEATKSIEPGSDYYELSDTRESVHESIDTMSPMHKNGDKEFQTGK
                     ESQPSPPAQEAGYSTLAQSYPSDLPEEPSSPQERMFTIDPKVYGEKRDLHSKNKDDLT
                     LSRSLGLGGRSAIEQRSMSINLPMSCLDSIALGFNFGRGHDLSPLASDILTNTSGSMD
                     EGDDYLPATTPALEKAPCFPVESKEEEQIEKVKATGEESTQAEISCESPFLAKDFYKN
                     GTVMAPDLPEMLDLAGTRSRLASVSADAEVARRKSVPSETVVEDSRTGLPPVTDENHV
                     IVKTDSQLEDLGYCVFNKYTVPLPSPVQDSENLSGESGTFYEGTDDKVRRDLATDLSL
                     IEVKLAAAGRVKDEFSVDKEASAHISGDKSGLSKEFDQEKKANDRLDTVLEKSEEHAD
                     SKEHAKKTEEAGDEIETFGLGVTYEQALAKDLSIPTDASSEKAEKGLSSVPEIAEVEP
                     SKKVEQGLDFAVQGQLDVKISDFGQMASGLNIDDRRATELKLEATQDMTPSSKAPQEA
                     DAFMGVESGHMKEGTKVSETEVKEKVAKPDLVHQEAVDKEESYESSGEHESLTMESLK
                     ADEGKKETSPESSLIQDEIAVKLSVEIPCPPAVSEADLATDERADVQMEFIQGPKEES
                     KETPDISITPSDVAEPLHETIVSEPAEIQSEEEEIEAQGEYDKLLFRSDTLQITDLGV
                     SGAREEFVETCPSEHKGVIESVVTIEDDFITVVQTTTDEGESGSHSVRFAALEQPEVE
                     RRPSPHDEEEFEVEEAAEAQAEPKDGSPEAPASPEREEVALSEYKTETYDDYKDETTI
                     DDSIMDADSLWVDTQDDDRSIMTEQLETIPKEEKAEKEARRSSLEKHRKEKPFKTGRG
                     RISTPERKVAKKEPSTVSRDEVRRKKAVYKKAELAKKTEVQAHSPSRKFILKPAIKYT
                     RPTHLSCVKRKTTAAGGESALAPSVFKQAKDKVSDGVTKSPEKRSSLPRPSSILPPRR
                     GVSGDRDENSFSLNSSISSSARRTTRSEPIRRAGKSGTSTPTTPGSTAITPGTPPSYS
                     SRTPGTPGTPSYPRTPHTPGTPKSAILVPSEKKVAIIRTPPKSPATPKQLRLINQPLP
                     DLKNVKSKIGSTDNIKYQPKGGQVQIVTKKIDLSHVTSKCGSLKNIRHRPGGGRVKIE
                     SVKLDFKEKAQAKVGSLDNAHHVPGGGNVKIDSQKLNFREHAKARVDHGAEIITQSPG
                     RSSVASPRRLSNVSSSGSINLLESPQLATLAEDVTAALAKQGL"
     exon            714..827
                     /gene="MAP2"
                     /gene_synonym="MAP-2; MAP2A; MAP2B; MAP2C"
                     /inference="alignment:Splign:2.1.0"
     exon            828..905
                     /gene="MAP2"
                     /gene_synonym="MAP-2; MAP2A; MAP2B; MAP2C"
                     /inference="alignment:Splign:2.1.0"
     exon            906..4631
                     /gene="MAP2"
                     /gene_synonym="MAP-2; MAP2A; MAP2B; MAP2C"
                     /inference="alignment:Splign:2.1.0"
     exon            4632..4838
                     /gene="MAP2"
                     /gene_synonym="MAP-2; MAP2A; MAP2B; MAP2C"
                     /inference="alignment:Splign:2.1.0"
     exon            4839..4973
                     /gene="MAP2"
                     /gene_synonym="MAP-2; MAP2A; MAP2B; MAP2C"
                     /inference="alignment:Splign:2.1.0"
     exon            4974..5035
                     /gene="MAP2"
                     /gene_synonym="MAP-2; MAP2A; MAP2B; MAP2C"
                     /inference="alignment:Splign:2.1.0"
     exon            5036..5183
                     /gene="MAP2"
                     /gene_synonym="MAP-2; MAP2A; MAP2B; MAP2C"
                     /inference="alignment:Splign:2.1.0"
     exon            5184..5524
                     /gene="MAP2"
                     /gene_synonym="MAP-2; MAP2A; MAP2B; MAP2C"
                     /inference="alignment:Splign:2.1.0"
     exon            5525..5606
                     /gene="MAP2"
                     /gene_synonym="MAP-2; MAP2A; MAP2B; MAP2C"
                     /inference="alignment:Splign:2.1.0"
     exon            5607..5719
                     /gene="MAP2"
                     /gene_synonym="MAP-2; MAP2A; MAP2B; MAP2C"
                     /inference="alignment:Splign:2.1.0"
     exon            5720..9650
                     /gene="MAP2"
                     /gene_synonym="MAP-2; MAP2A; MAP2B; MAP2C"
                     /inference="alignment:Splign:2.1.0"
     regulatory      9629..9634
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MAP2"
                     /gene_synonym="MAP-2; MAP2A; MAP2B; MAP2C"
                     /note="hexamer: AATAAA"
     polyA_site      9650
                     /gene="MAP2"
                     /gene_synonym="MAP-2; MAP2A; MAP2B; MAP2C"
                     /note="major polyA site"
ORIGIN      
        1 agtctggggc gggcgctcgg gctgcgcggg ctctgggcag cagcagcagc agcagcagca
       61 tcctctcttc ctttacttcc cttccgcttc tttctcttcc ttctccttct ttttcccccc
      121 cctccccttc ttcccctaac ccttctaccc ctctcctttt tctccggagg gcgctaagtc
      181 cgtgagcggt ggcagtcgcg accgcgggtg catccagttt ctgcgcccag attttattga
      241 tctaatccaa agtatcttat aacttctggc tggaattaag attcttcagc ttgtctctaa
      301 ccgaggaagc attgattggg agctactcat tcagaaaatt aaaagaaaga agccagaaaa
      361 tattatcaac cctttgagaa cacgacacaa cgaactttat attttaccac ttccttgaat
      421 agttgcagga gaaataacaa ggcattgaag aatggcagat gaacggaaag atgaagcaaa
      481 ggcacctcac tggacctcag caccgctaac agaggcatct gcacactcac atccacctga
      541 gattaaggat caaggcggag caggggaagg acttgtccga agcgccaatg gattcccata
      601 cagggaggat gaagagggtg cctttggaga gcatgggtca cagggcacct attcaaatac
      661 caaagagaat gggatcaacg gagagctgac ctcagctgac agagaaacag cagaggaggt
      721 gtctgcaagg atagttcaag tagtcactgc tgaggctgta gcagtcctga aaggtgaaca
      781 agagaaagaa gctcaacata aagaccagac tgcagctctg cctttagcag ctgaagaaac
      841 agctaatctg cctccttctc cacccccatc acctgcctca gaacagactg tcacagtgga
      901 ggaagattta cttacagcct cgaagatgga gttccacgat caacaggaat tgactccctc
      961 tacagctgag ccttcagacc agaaggaaaa ggagtcagag aagcaaagta agcctggtga
     1021 agaccttaaa catgctgcct tagtttctca gccagagaca actaaaactt accctgataa
     1081 aaaggacatg caaggcacgg aagaagaaaa agcaccccta gctttgtttg ggcacactct
     1141 tgttgccagc ctggaagaca tgaaacagaa gacagaacca agccttgtag tacctggcat
     1201 tgacctccct aaagagcctc caactccaaa agaacaaaag gactggttca tcgaaatgcc
     1261 aacggaagca aaaaaggatg agtggggttt agttgccccc atatctcctg gccctctgac
     1321 tcccatgagg gaaaaagatg tatttgatga tatcccaaaa tgggaaggga aacagtttga
     1381 ttctcccatg ccaagtccct ttcaaggggg aagcttcact cttcctttag atgtcatgaa
     1441 gaatgaaata gttacagaaa catcgccctt tgcccctgcc tttttacagc cagatgacaa
     1501 aaaatctctg caacaaacca gtggcccagc tactgccaaa gatagtttta aaattgaaga
     1561 gccccatgag gctaaacctg acaaaatggc agaagcacca ccctcagagg caatgacctt
     1621 acccaaagat gctcacattc cagttgtaga agaacatgtt atggggaaag ttttagagga
     1681 agaaaaggag gccataaatc aagagactgt gcagcaaagg gatactttca cccccagtgg
     1741 acaggaacct atacttactg aaaaggaaac tgagctgaag cttgaagaaa aaaccaccat
     1801 ttctgacaaa gaagctgtgc caaaagagag taaaccccca aaacctgcag atgaagaaat
     1861 aggcataatt cagacctcca cagagcacac tttctcagaa cagaaagacc aagagcctac
     1921 cacagatatg ttgaaacagg actcgttccc tgtaagtttg gagcaagcag ttacagattc
     1981 agccatgacc tctaaaacac tggagaaagc catgaccgaa ccatctgcat taattgaaaa
     2041 gagctcaatt caggaacttt ttgaaatgag agttgatgac aaagataaga ttgaaggagt
     2101 tggagctgca acatcagctg agcttgatat gccattttat gaagataaat caggaatgtc
     2161 caagtacttt gaaacatctg ccttgaaaga agaagcaaca aaaagcattg agccaggcag
     2221 tgattactat gaactgagtg acactagaga aagtgtccat gagtctattg ataccatgtc
     2281 tcccatgcat aaaaatggtg acaaggagtt tcaaacagga aaagaatccc agcccagtcc
     2341 tccagcacaa gaagcagggt acagcactct cgcacagagt tatccatcag atttacctga
     2401 agaacccagt tctcctcaag aaagaatgtt cactattgat ccaaaagtgt atggagagaa
     2461 aagggacctc cacagtaaga ataaggatga tttgaccctt agcaggagtt taggacttgg
     2521 tggtaggtct gcaatagaac aaagaagcat gtcaatcaat ttgccgatgt cttgcctaga
     2581 ttccatagcc cttggattta actttggtcg gggacatgat ctttctcctc tggcttccga
     2641 tattctaacc aacactagtg gaagtatgga tgaaggggat gattaccttc cagccaccac
     2701 acctgcactg gagaaagccc cttgcttccc tgtagaaagc aaagaggaag aacagataga
     2761 gaaagtaaaa gctactggag aagaaagtac tcaagcggag atatcatgtg agtctccttt
     2821 cctagccaaa gatttttaca aaaatggtac tgtcatggca cctgaccttc ctgaaatgct
     2881 agatctggca ggcacaaggt caagattggc ttctgtgagt gcagatgctg aggttgccag
     2941 gaggaaatca gtcccatcag agactgtggt tgaggatagt cgtactggct tgcccccggt
     3001 aactgatgaa aaccatgtca ttgtaaaaac ggacagtcag ctcgaagacc tgggctactg
     3061 tgtgttcaat aagtacacag tcccattgcc atcacctgtt caagacagtg agaatttatc
     3121 aggggagagt ggtacctttt acgaaggcac tgatgataaa gttcgaagag atttggccac
     3181 agacctttca ctgattgaag tgaaactggc agcagccgga agagtcaaag atgagttcag
     3241 tgttgacaaa gaagcatccg cgcatatctc tggtgacaaa tcaggactga gtaaggagtt
     3301 tgaccaagag aagaaagcta atgataggtt ggatactgta ctagaaaaga gtgaagaaca
     3361 tgctgattca aaagaacatg ccaagaaaac tgaagaggct ggtgatgaaa tagaaacatt
     3421 cggattagga gtaacctatg agcaagcttt ggccaaagat ttgtcaatac caacagatgc
     3481 atcctctgag aaagcagaga agggtcttag ttcagtgcca gagatagctg aggtagaacc
     3541 atccaaaaag gtggaacaag gtctggattt tgctgtccag ggtcaactag atgttaaaat
     3601 tagtgacttt ggacagatgg cttcagggct aaacatagat gatagaaggg caacagagct
     3661 aaaacttgag gctacacagg acatgacccc ctcatccaaa gcaccgcagg aggcagatgc
     3721 atttatgggt gttgagtctg gccacatgaa agaaggcact aaagttagtg agacagaagt
     3781 caaagagaag gtggccaagc ctgacttggt gcaccaggag gctgtagaca aggaggagtc
     3841 ctatgaatct agtggtgagc atgaaagtct caccatggag tccttgaaag ctgatgaggg
     3901 caagaaggaa acatctccag aatcatctct aattcaagat gagattgccg tcaaattgtc
     3961 agtggaaata ccttgcccac ctgctgtttc agaggctgat ttagccacag atgagagagc
     4021 tgatgtccag atggaattta ttcaggggcc aaaagaagaa agcaaagaga ccccagatat
     4081 atccatcacg ccttctgatg ttgcagagcc attgcatgaa acgatcgtat ctgaaccagc
     4141 agagattcag agtgaggaag aagagataga agcccaggga gaatatgata aactgctctt
     4201 ccgctcagac acccttcaga taactgacct gggtgtctca ggtgccaggg aggaatttgt
     4261 ggagacctgc ccaagtgaac acaaaggagt gattgagtct gttgtgacca tcgaggatga
     4321 tttcatcact gtagtgcaaa ccacaactga tgaaggggag tcagggtccc acagcgtgcg
     4381 ttttgcagcc ctagagcagc ctgaggtgga aaggagacca tctcctcatg atgaagaaga
     4441 gtttgaagta gaagaggcag ctgaagccca ggcagaaccc aaagatggtt ccccagaggc
     4501 tccagcttcc cctgagagag aagaggttgc actttctgaa tataagacag aaacctatga
     4561 cgattacaaa gatgagacca ccattgacga ctccatcatg gacgctgaca gcctctgggt
     4621 ggacactcaa gatgatgata ggagcatcat gacagaacag ttagaaacta ttcctaaaga
     4681 ggagaaagct gaaaaggaag ctcggagatc atctcttgag aaacatagaa aagaaaagcc
     4741 ttttaaaacc gggagaggca gaatttccac tcctgaaaga aaagtagcta aaaaggaacc
     4801 tagcacagtc tccagagatg aagtgagaag gaaaaaagca gtttataaga aggctgaact
     4861 tgctaaaaaa acagaagttc aggcccactc tccctccagg aaattcattt taaaacctgc
     4921 tatcaaatat actagaccaa ctcatctctc ctgtgttaag cggaaaacca cagcagcagg
     4981 tggggaatca gctctggctc ccagtgtatt taaacaggca aaggacaaag tctctgacgg
     5041 agtaaccaag agcccagaaa agcgctcttc tctcccaaga ccttcctcca ttctccctcc
     5101 tcggcgaggt gtgtcaggag acagagatga gaattccttc tctctcaaca gttctatctc
     5161 ttcttcagca cggcggacca ccaggtcaga gccaattcgc agagcaggga agagtggtac
     5221 ctcaacaccc actacccctg ggtctactgc catcactcct ggcaccccac caagttattc
     5281 ttcacgcaca ccaggcactc ctggaacccc tagctatccc aggacccctc acacaccagg
     5341 aacccccaag tctgccatct tggtgccgag tgagaagaag gtcgccatca tacgtactcc
     5401 tccaaaatct cctgcgactc ccaagcagct tcggcttatt aaccaaccac tgccagacct
     5461 gaagaatgtc aaatccaaaa tcggatcaac agacaacatc aaataccagc ctaaaggggg
     5521 gcaggtacaa attgttacca agaaaataga cctaagccat gtgacatcca aatgtggctc
     5581 tctgaagaac atccgccaca ggccaggtgg cggacgtgtg aaaattgaga gtgtaaaact
     5641 agatttcaaa gaaaaggccc aagctaaagt tggttctctt gataatgctc atcatgtacc
     5701 tggaggtggt aatgtcaaga ttgacagcca aaagttgaac ttcagagagc atgctaaagc
     5761 ccgtgtggac catggggctg agatcattac acagtcccca ggcagatcca gcgtggcatc
     5821 accccgacga ctcagcaatg tctcctcgtc tggaagcatc aacctgctcg aatctcctca
     5881 gcttgccact ttggctgagg atgtcactgc tgcactcgct aagcagggct tgtgaatatt
     5941 tctcatttag cattgaaata ataatattta ggcatgagct cttggcagga gtgggctctg
     6001 agcagttgtt atattcattc tttataaacc ataaaataaa taatctcatc cccaaactgt
     6061 agtaattgtt acaattttct atttaaaaaa tgaatagtac atgcagaaat tgacctgatt
     6121 tccatttgca acaggaagac actggcttta catgggttca attggacaat tatttttgct
     6181 ctgctctgtt ttgcatggag tattattatt ttaaaaattg catttttacc tttcatgtgc
     6241 ctgaaggcta tccactacat tctgaaggcc ttgttaaaat ccaagctgct catttcacta
     6301 ttctgtttct gagtgagaag ataaaaactg cccattgtaa cttatttcag gttaaattaa
     6361 accaaggagt ctgattgcag gaagggaaga gcatgtaaga aataagtttt tttaaagtgt
     6421 tattttgtat aaatgggaag aaagattcaa ttaagttatt aacatttggg acctggataa
     6481 ttatatcaga gtatgtcagt ccaataaatt atttaactaa ttaaaaaata gttgcaaagc
     6541 atttgagctg tggttgagga agtggtgtaa aagtgcatcc attaggaatg atgcactttc
     6601 attaggatgg actcgtgtct gattagaatg tcagttgatc agctagattt gtgtccacac
     6661 taccagtttc acaccccctt tccatctgtt tgatacagta ttatagatat aaatatatat
     6721 atatttctct gtggccattt gtgatacttc ctcatatact tgaatattat acttctttat
     6781 tcacagtatc tgtgtctcct gcaccctttg gtgttgcaat tttagatatg tgaaagtaga
     6841 tgttagcagg gttctctccc tatttaaaaa aaatacatta aaaaagacaa aaaattttag
     6901 catgaagttg ctttctgtaa caactcaaag ccgtaaccct gttttagtgc cagatacaag
     6961 tctctcccgt gatgctagac aaaaaattat ttttctttgc tttcaccaac atggagtttg
     7021 tgggggtggg tccagttata catgaaaggg tttacagatt gttggtttaa gattatggat
     7081 ttatctcatt tttaatcaca ggatagtttg gggtttattc ctattattat tcatgaaacc
     7141 gacttaagat tttttcttta tttttctttt tttttccatt tgctaaagtt gaaagttgaa
     7201 actaactata atagtttgaa acatgttttc tcatttttcc aaatagtatc tgtttattaa
     7261 attctctaat agaagatgtt tgtctttctt acccaaagta aagatcccct gatcagaaag
     7321 aaaaaataca atactttggg aagctatagc tataaaacac ttgagacaca gatatctaaa
     7381 tcagtttttt tccaagactc caacattgca ctctgtaaag taacacactg tgatctagta
     7441 ttatttatca gtagataata ctgttctgac tgtatataca gtctagaact cacaaatcaa
     7501 ttagttcctc tcacaaatca ttcatcttag acttacaaat aaggaatgaa atagtcaatg
     7561 gcctgattaa ggcaaagagc taccaggcta gatggacact ttttaaaaat tttatctgtt
     7621 ctttttcttg ctcagggctg gtaggttgga tctgaaccat taaaatcaaa tggtccacta
     7681 ggcgtatgat ctctttgagc caaatcagtt cctgaatata aaggaggaaa tgatgaggat
     7741 gtactgaggc aacggggaag tatagaaaca tccaagacaa aagccaaggg atgcaaaggc
     7801 agagacacag gtgctttttg gtgacccagt ggatatggca accagtgtaa ctgccataca
     7861 agaaacccta ggagcaaacc cacaccactc attctcagct aagagatttt acacaggcaa
     7921 acgtgtctta aaccatctat aaatcagtta ttttatatga cagtcaaaac cttagaaacc
     7981 ttaggatcat tatatctatt ttctgcctat taattgctgt gaggtttgat ttgaccaatc
     8041 tgggcaattt attcatcagc ttcccttgaa gtgcaccaga aaatagaaga aaggtgtgtg
     8101 gagacttagg gtattttatt acatgttttc atagtcttaa atagtgatta aatttctcta
     8161 gaaagaagtt aacagctcat tagaaaagtt ttaacctgtg aaataagtat ttttctcaac
     8221 attctttaaa gtttttatat aagttaacac taggtaaaca ttctgcatac tagaagtcag
     8281 tttattacaa atacatgtca aaaataaaga ttatacaagg caccaaacta ctagatttgg
     8341 cattaaaaca aatgtttatt tctaatcaca acaaaattat aatgaataaa tgttcttgct
     8401 ttgtatggaa atacaattct ttattaaagt taacagaaag gaactgatcg tttgtaccag
     8461 taaaagagag aaacacacag gttaaatatc ttcttgtggg gttaaggggt agaacctatc
     8521 ttgccttcac tctcaagata acgactcaaa ttaagctttt tgagcaccac tcttgtgggg
     8581 acacacatac gctgatctag gaatgaaatc ttcgtggtct caattctaga tctactatgc
     8641 cagtttctct ctggctttag cctttgagaa cctgtataag aatacgtaag taatccagag
     8701 ctgtgaagag tttaaaggcc aacttctcca gtgaactcaa cctctgggtc acttgcaacc
     8761 agaaattgga tacctcataa tgatgcagga aagacccgag ttcatgatga gtttcaaagg
     8821 ccacgttcat ttaggaacca actctctctg gatttacctg ctgagttcca gcagcgtgat
     8881 gggctgacat cccacctaca agtatgacac ctgtgtaaca ccagctaggt acggctggag
     8941 aaggctgaag agagaatgcc attaaatgga agaatgtact gattgtagtg accttctcca
     9001 cacacacaca cacacacaca cacacacaca cctacagtaa tacagcaagc gtggaataat
     9061 cagccaatat ataacattcc atcagtattt tattaaggaa ataacctgaa tgtggttgat
     9121 tttgacatag ctgcaattac agttttcttc tatttttcaa gccacaataa ggaaaataaa
     9181 ctactcatgg tctaaatact agagataaag tagattcatg gcttggtaag gaaattttaa
     9241 gcattccttc aaagattgac gtgctaaaat aagcattgat gttttgagtt tttttacacc
     9301 taggattttt agcttgggtg tgtaggtgaa ggccaagact ctctgcagga aaaagcttat
     9361 tttcaaactc agaaaataaa atgtcaatca taaaaatcta cttcaacttt agcaaaaaga
     9421 aaaaaaaatc aacaaaaagt atactctgta tgctgggatt ccgaggttcc aacacactgt
     9481 tacaaatctg tggggggttt ctttcttctg ataattctag agcctgttac catagaaagg
     9541 catttcttca atggctggtt gtagttagtt catgtttttc aatcaaattt gcaaatgtat
     9601 ttgttgctgt atagtgattg ttttgcaaaa taaaattgct tgtcacctaa
//
