LOCUS       NM_006010                909 bp    mRNA    linear   PRI 05-AUG-2024
DEFINITION  Homo sapiens mesencephalic astrocyte derived neurotrophic factor
            (MANF), mRNA.
ACCESSION   NM_006010
VERSION     NM_006010.6
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 909)
  AUTHORS   Wang,F., Han,X., Mu,Q., Chen,H., Wu,Y., Kang,Y. and Liu,Y.
  TITLE     Cerebrospinal fluid mesencephalic astrocyte-derived neurotrophic
            factor: A moderating effect on sleep time and cognitive function
  JOURNAL   J Psychiatr Res 176, 33-39 (2024)
   PUBMED   38838432
  REMARK    GeneRIF: Cerebrospinal fluid mesencephalic astrocyte-derived
            neurotrophic factor: A moderating effect on sleep time and
            cognitive function.
REFERENCE   2  (bases 1 to 909)
  AUTHORS   Huang,M., Hu,J., Chen,Y., Xun,Y., Zhang,X. and Cao,Y.
  TITLE     Mesencephalic astrocyte-derived neurotrophic factor inhibits
            cervical cancer progression via regulating macrophage phenotype
  JOURNAL   Mol Biol Rep 51 (1), 654 (2024)
   PUBMED   38735002
  REMARK    GeneRIF: Mesencephalic astrocyte-derived neurotrophic factor
            inhibits cervical cancer progression via regulating macrophage
            phenotype.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 909)
  AUTHORS   Anttila,J.E., Mattila,O.S., Liew,H.K., Matlik,K., Mervaala,E.,
            Lindholm,P., Lindahl,M., Lindsberg,P.J., Tseng,K.Y. and
            Airavaara,M.
  TITLE     MANF protein expression is upregulated in immune cells in the
            ischemic human brain and systemic recombinant MANF delivery in rat
            ischemic stroke model demonstrates anti-inflammatory effects
  JOURNAL   Acta Neuropathol Commun 12 (1), 10 (2024)
   PUBMED   38229173
  REMARK    GeneRIF: MANF protein expression is upregulated in immune cells in
            the ischemic human brain and systemic recombinant MANF delivery in
            rat ischemic stroke model demonstrates anti-inflammatory effects.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 909)
  AUTHORS   Parkash,V., Lindholm,P., Peranen,J., Kalkkinen,N., Oksanen,E.,
            Saarma,M., Leppanen,V.M. and Goldman,A.
  TITLE     The structure of the conserved neurotrophic factors MANF and CDNF
            explains why they are bifunctional
  JOURNAL   Protein Eng Des Sel 22 (4), 233-241 (2009)
   PUBMED   19258449
  REMARK    GeneRIF: structures of MANF and CDNF were solved; structure
            explains why MANF and CDNF are bifunctional; neurotrophic activity
            may reside in the N-terminal domain and ER stress response in the
            C-terminal domain
REFERENCE   5  (bases 1 to 909)
  AUTHORS   Petrova,P., Raibekas,A., Pevsner,J., Vigo,N., Anafi,M., Moore,M.K.,
            Peaire,A.E., Shridhar,V., Smith,D.I., Kelly,J., Durocher,Y. and
            Commissiong,J.W.
  TITLE     MANF: a new mesencephalic, astrocyte-derived neurotrophic factor
            with selectivity for dopaminergic neurons
  JOURNAL   J Mol Neurosci 20 (2), 173-188 (2003)
   PUBMED   12794311
REFERENCE   6  (bases 1 to 909)
  AUTHORS   Tanaka,H., Shimada,Y., Harada,H., Shinoda,M., Hatooka,S.,
            Imamura,M. and Ishizaki,K.
  TITLE     Polymorphic variation of the ARP gene on 3p21 in Japanese
            esophageal cancer patients
  JOURNAL   Oncol Rep 7 (3), 591-593 (2000)
   PUBMED   10767373
REFERENCE   7  (bases 1 to 909)
  AUTHORS   Evron,E., Cairns,P., Halachmi,N., Ahrendt,S.A., Reed,A.L. and
            Sidransky,D.
  TITLE     Normal polymorphism in the incomplete trinucleotide repeat of the
            arginine-rich protein gene
  JOURNAL   Cancer Res 57 (14), 2888-2889 (1997)
   PUBMED   9230196
REFERENCE   8  (bases 1 to 909)
  AUTHORS   Shridhar,V., Rivard,S., Wang,X., Shridhar,R., Paisley,C.,
            Mullins,C., Beirnat,L., Dugan,M., Sarkar,F., Miller,O.J.,
            Vaitkevicius,V.K. and Smith,D.I.
  TITLE     Mutations in the arginine-rich protein gene (ARP) in pancreatic
            cancer
  JOURNAL   Oncogene 14 (18), 2213-2216 (1997)
   PUBMED   9174057
REFERENCE   9  (bases 1 to 909)
  AUTHORS   Shridhar,R., Shridhar,V., Rivard,S., Siegfried,J.M.,
            Pietraszkiewicz,H., Ensley,J., Pauley,R., Grignon,D., Sakr,W.,
            Miller,O.J. and Smith,D.I.
  TITLE     Mutations in the arginine-rich protein gene, in lung, breast, and
            prostate cancers, and in squamous cell carcinoma of the head and
            neck
  JOURNAL   Cancer Res 56 (24), 5576-5578 (1996)
   PUBMED   8971156
REFERENCE   10 (bases 1 to 909)
  AUTHORS   Shridhar,V., Rivard,S., Shridhar,R., Mullins,C., Bostick,L.,
            Sakr,W., Grignon,D., Miller,O.J. and Smith,D.I.
  TITLE     A gene from human chromosomal band 3p21.1 encodes a highly
            conserved arginine-rich protein and is mutated in renal cell
            carcinomas
  JOURNAL   Oncogene 12 (9), 1931-1939 (1996)
   PUBMED   8649854
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from M83751.1 and BC007282.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Sep 20, 2018 this sequence version replaced NM_006010.5.
            
            Summary: The protein encoded by this gene is localized in the
            endoplasmic reticulum (ER) and golgi, and is also secreted.
            Reducing expression of this gene increases susceptibility to ER
            stress-induced death and results in cell proliferation. Activity of
            this protein is important in promoting the survival of dopaminergic
            neurons. The presence of polymorphisms in the N-terminal
            arginine-rich region, including a specific mutation that changes an
            ATG start codon to AGG, have been reported in a variety of solid
            tumors; however, these polymorphisms were later shown to exist in
            normal tissues and are thus no longer thought to be tumor-related.
            [provided by RefSeq, Apr 2014].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC007282.1, SRR7346977.531546.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            CDS uses downstream in-frame AUG :: upstream AUG and CDS extension
                                                is not conserved
            MANE Ensembl match               :: ENST00000528157.7/
                                                ENSP00000432799.3
            RefSeq Select criteria           :: based on single protein-coding
                                                transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-50                M83751.1           237-286
            51-909              BC007282.1         1-859
FEATURES             Location/Qualifiers
     source          1..909
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="3"
                     /map="3p21.2"
     gene            1..909
                     /gene="MANF"
                     /gene_synonym="ARMET; ARP; DDDS"
                     /note="mesencephalic astrocyte derived neurotrophic
                     factor"
                     /db_xref="GeneID:7873"
                     /db_xref="HGNC:HGNC:15461"
                     /db_xref="MIM:601916"
     exon            1..146
                     /gene="MANF"
                     /gene_synonym="ARMET; ARP; DDDS"
                     /inference="alignment:Splign:2.1.0"
     CDS             53..601
                     /gene="MANF"
                     /gene_synonym="ARMET; ARP; DDDS"
                     /note="arginine-rich, mutated in early stage tumors"
                     /codon_start=1
                     /product="mesencephalic astrocyte-derived neurotrophic
                     factor precursor"
                     /protein_id="NP_006001.5"
                     /db_xref="CCDS:CCDS46836.4"
                     /db_xref="GeneID:7873"
                     /db_xref="HGNC:HGNC:15461"
                     /db_xref="MIM:601916"
                     /translation="MRRMWATQGLAVALALSVLPGSRALRPGDCEVCISYLGRFYQDL
                     KDRDVTFSPATIENELIKFCREARGKENRLCYYIGATDDAATKIINEVSKPLAHHIPV
                     EKICEKLKKKDSQICELKYDKQIDLSTVDLKKLRVKELKKILDDWGETCKGCAEKSDY
                     IRKINELMPKYAPKAASARTDL"
     exon            147..274
                     /gene="MANF"
                     /gene_synonym="ARMET; ARP; DDDS"
                     /inference="alignment:Splign:2.1.0"
     exon            275..416
                     /gene="MANF"
                     /gene_synonym="ARMET; ARP; DDDS"
                     /inference="alignment:Splign:2.1.0"
     exon            417..909
                     /gene="MANF"
                     /gene_synonym="ARMET; ARP; DDDS"
                     /inference="alignment:Splign:2.1.0"
     polyA_site      661
                     /gene="MANF"
                     /gene_synonym="ARMET; ARP; DDDS"
                     /note="major polyA site"
     regulatory      830..835
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MANF"
                     /gene_synonym="ARMET; ARP; DDDS"
                     /note="hexamer: ATTAAA"
     regulatory      834..839
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MANF"
                     /gene_synonym="ARMET; ARP; DDDS"
                     /note="hexamer: AAGAAA"
     polyA_site      856
                     /gene="MANF"
                     /gene_synonym="ARMET; ARP; DDDS"
                     /note="major polyA site"
     regulatory      865..870
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MANF"
                     /gene_synonym="ARMET; ARP; DDDS"
                     /note="hexamer: ATTATA"
     polyA_site      865
                     /gene="MANF"
                     /gene_synonym="ARMET; ARP; DDDS"
     polyA_site      909
                     /gene="MANF"
                     /gene_synonym="ARMET; ARP; DDDS"
ORIGIN      
        1 agtcggtcgg cggcggcagc ggaggaggag gaggaggagg aggatgagga ggatgaggag
       61 gatgtgggcc acgcaggggc tggcggtggc gctggctctg agcgtgctgc cgggcagccg
      121 ggcgctgcgg ccgggcgact gcgaagtttg tatttcttat ctgggaagat tttaccagga
      181 cctcaaagac agagatgtca cattctcacc agccactatt gaaaacgaac ttataaagtt
      241 ctgccgggaa gcaagaggca aagagaatcg gttgtgctac tatatcgggg ccacagatga
      301 tgcagccacc aaaatcatca atgaggtatc aaagcctctg gcccaccaca tccctgtgga
      361 gaagatctgt gagaagctta agaagaagga cagccagata tgtgagctta agtatgacaa
      421 gcagatcgac ctgagcacag tggacctgaa gaagctccga gttaaagagc tgaagaagat
      481 tctggatgac tggggggaga catgcaaagg ctgtgcagaa aagtctgact acatccggaa
      541 gataaatgaa ctgatgccta aatatgcccc caaggcagcc agtgcacgga ccgatttgta
      601 gtctgctcaa tctctgttgc acctgagggg gaaaaaacag ttcaactgct tactcccaaa
      661 acagcctttt tgtaatttat tttttaagtg ggctcctgac aatactgtat cagatgtgaa
      721 gcctggagct ttcctgatga tgctggccct acagtacccc catgagggga ttcccttcct
      781 tctgttgctg gtgtactcta ggacttcaaa gtgtgtctgg gattttttta ttaaagaaaa
      841 aaaatttcta gctgtccttg cagaattata gtgaatacca aaatggggtt ttgccccagg
      901 aggctccta
//
