LOCUS       NM_002372               6553 bp    mRNA    linear   PRI 07-APR-2024
DEFINITION  Homo sapiens mannosidase alpha class 2A member 1 (MAN2A1), mRNA.
ACCESSION   NM_002372
VERSION     NM_002372.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6553)
  AUTHORS   Shi,S., Gu,S., Han,T., Zhang,W., Huang,L., Li,Z., Pan,D., Fu,J.,
            Ge,J., Brown,M., Zhang,P., Jiang,P., Wucherpfennig,K.W. and
            Liu,X.S.
  TITLE     Inhibition of MAN2A1 Enhances the Immune Response to Anti-PD-L1 in
            Human Tumors
  JOURNAL   Clin Cancer Res 26 (22), 5990-6002 (2020)
   PUBMED   32723834
  REMARK    GeneRIF: Inhibition of MAN2A1 Enhances the Immune Response to
            Anti-PD-L1 in Human Tumors.
REFERENCE   2  (bases 1 to 6553)
  AUTHORS   Li,C., Guo,L., Chen,F., Yu,W., Rao,T. and Ruan,Y.
  TITLE     Golgi Alpha-Mannosidase II as a Novel Biomarker Predicts Prognosis
            in Clear Cell Renal Cell Carcinoma
  JOURNAL   Oncol Res Treat 43 (6), 264-275 (2020)
   PUBMED   32403105
  REMARK    GeneRIF: Golgi Alpha-Mannosidase II as a Novel Biomarker Predicts
            Prognosis in Clear Cell Renal Cell Carcinoma.
REFERENCE   3  (bases 1 to 6553)
  AUTHORS   Chen,Z.H., Yu,Y.P., Tao,J., Liu,S., Tseng,G., Nalesnik,M.,
            Hamilton,R., Bhargava,R., Nelson,J.B., Pennathur,A., Monga,S.P.,
            Luketich,J.D., Michalopoulos,G.K. and Luo,J.H.
  TITLE     MAN2A1-FER Fusion Gene Is Expressed by Human Liver and Other Tumor
            Types and Has Oncogenic Activity in Mice
  JOURNAL   Gastroenterology 153 (4), 1120-1132 (2017)
   PUBMED   28245430
  REMARK    GeneRIF: Many human tumor types and cancer cell lines express the
            MAN2A1-FER fusion, which increases proliferation and invasiveness
            of cancer cell lines and has liver oncogenic activity in mice.
REFERENCE   4  (bases 1 to 6553)
  AUTHORS   Bobovska,A., Tvaroska,I. and Kona,J.
  TITLE     Using DFT methodology for more reliable predictive models: Design
            of inhibitors of Golgi alpha-Mannosidase II
  JOURNAL   J Mol Graph Model 66, 47-57 (2016)
   PUBMED   27035259
  REMARK    GeneRIF: he QSAR models with the fragmented QM-DFT descriptors may
            find a useful application in structure-based drug design where pure
            empirical and force field methods reached their limits and where
            quantum mechanics effects are critical for ligand-receptor
            interactions. The optimized models will apply in lead optimization
            processes for GMII drug developments.
REFERENCE   5  (bases 1 to 6553)
  CONSRTM   Schizophrenia Working Group of the Psychiatric Genomics Consortium
  TITLE     Biological insights from 108 schizophrenia-associated genetic loci
  JOURNAL   Nature 511 (7510), 421-427 (2014)
   PUBMED   25056061
REFERENCE   6  (bases 1 to 6553)
  AUTHORS   Misago,M., Liao,Y.F., Kudo,S., Eto,S., Mattei,M.G., Moremen,K.W.
            and Fukuda,M.N.
  TITLE     Molecular cloning and expression of cDNAs encoding human
            alpha-mannosidase II and a previously unrecognized
            alpha-mannosidase IIx isozyme
  JOURNAL   Proc Natl Acad Sci U S A 92 (25), 11766-11770 (1995)
   PUBMED   8524845
REFERENCE   7  (bases 1 to 6553)
  AUTHORS   Moremen,K.W. and Robbins,P.W.
  TITLE     Isolation, characterization, and expression of cDNAs encoding
            murine alpha-mannosidase II, a Golgi enzyme that controls
            conversion of high mannose to complex N-glycans
  JOURNAL   J Cell Biol 115 (6), 1521-1534 (1991)
   PUBMED   1757461
REFERENCE   8  (bases 1 to 6553)
  AUTHORS   Moremen,K.W., Touster,O. and Robbins,P.W.
  TITLE     Novel purification of the catalytic domain of Golgi
            alpha-mannosidase II. Characterization and comparison with the
            intact enzyme
  JOURNAL   J Biol Chem 266 (25), 16876-16885 (1991)
   PUBMED   1885615
REFERENCE   9  (bases 1 to 6553)
  AUTHORS   Fukuda,M.N., Masri,K.A., Dell,A., Luzzatto,L. and Moremen,K.W.
  TITLE     Incomplete synthesis of N-glycans in congenital dyserythropoietic
            anemia type II caused by a defect in the gene encoding
            alpha-mannosidase II
  JOURNAL   Proc Natl Acad Sci U S A 87 (19), 7443-7447 (1990)
   PUBMED   2217175
REFERENCE   10 (bases 1 to 6553)
  AUTHORS   Moremen,K.W. and Touster,O.
  TITLE     Biosynthesis and modification of Golgi mannosidase II in HeLa and
            3T3 cells
  JOURNAL   J Biol Chem 260 (11), 6654-6662 (1985)
   PUBMED   3922977
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BC111062.1, U31520.1, D63998.1,
            AC011366.6 and AL833486.1.
            
            On Aug 11, 2020 this sequence version replaced NM_002372.3.
            
            Summary: This gene encodes a glycosyl hydrolase that localizes to
            the Golgi and catalyzes the final hydrolytic step in the
            asparagine-linked oligosaccharide (N-glycan) maturation pathway.
            Mutations in the mouse homolog of this gene have been shown to
            cause a systemic autoimmune disease similar to human systemic lupus
            erythematosus. [provided by RefSeq, Dec 2013].
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR11853567.24383.1,
                                           SRR11853565.23447.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000261483.5/ ENSP00000261483.4
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-625               BC111062.1         524-1148
            626-1735            U31520.1           138-1247
            1736-1939           D63998.1           1755-1958
            1940-2312           U31520.1           1452-1824
            2313-3307           D63998.1           2332-3326
            3308-4066           U31520.1           2820-3578
            4067-5334           AC011366.6         131149-132416       c
            5335-6553           AL833486.1         3203-4421
FEATURES             Location/Qualifiers
     source          1..6553
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="5"
                     /map="5q21.3"
     gene            1..6553
                     /gene="MAN2A1"
                     /gene_synonym="AMan II; GOLIM7; MANA2; MANII"
                     /note="mannosidase alpha class 2A member 1"
                     /db_xref="GeneID:4124"
                     /db_xref="HGNC:HGNC:6824"
                     /db_xref="MIM:154582"
     exon            1..626
                     /gene="MAN2A1"
                     /gene_synonym="AMan II; GOLIM7; MANA2; MANII"
                     /inference="alignment:Splign:2.1.0"
     CDS             492..3926
                     /gene="MAN2A1"
                     /gene_synonym="AMan II; GOLIM7; MANA2; MANII"
                     /EC_number="3.2.1.114"
                     /note="mannosidase, alpha type II; golgi integral membrane
                     protein 7; Golgi alpha-mannosidase II;
                     mannosyl-oligosaccharide 1,3-1,6-alpha-mannosidase"
                     /codon_start=1
                     /product="alpha-mannosidase 2"
                     /protein_id="NP_002363.2"
                     /db_xref="CCDS:CCDS34209.1"
                     /db_xref="GeneID:4124"
                     /db_xref="HGNC:HGNC:6824"
                     /db_xref="MIM:154582"
                     /translation="MKLSRQFTVFGSAIFCVVIFSLYLMLDRGHLDYPRNPRREGSFP
                     QGQLSMLQEKIDHLERLLAENNEIISNIRDSVINLSESVEDGPKSSQSNFSQGAGSHL
                     LPSQLSLSVDTADCLFASQSGSHNSDVQMLDVYSLISFDNPDGGVWKQGFDITYESNE
                     WDTEPLQVFVVPHSHNDPGWLKTFNDYFRDKTQYIFNNMVLKLKEDSRRKFIWSEISY
                     LSKWWDIIDIQKKDAVKSLIENGQLEIVTGGWVMPDEATPHYFALIDQLIEGHQWLEN
                     NIGVKPRSGWAIDPFGHSPTMAYLLNRAGLSHMLIQRVHYAVKKHFALHKTLEFFWRQ
                     NWDLGSVTDILCHMMPFYSYDIPHTCGPDPKICCQFDFKRLPGGRFGCPWGVPPETIH
                     PGNVQSRARMLLDQYRKKSKLFRTKVLLAPLGDDFRYCEYTEWDLQFKNYQQLFDYMN
                     SQSKFKVKIQFGTLSDFFDALDKADETQRDKGQSMFPVLSGDFFTYADRDDHYWSGYF
                     TSRPFYKRMDRIMESHLRAAEILYYFALRQAHKYKINKFLSSSLYTALTEARRNLGLF
                     QHHDAITGTAKDWVVVDYGTRLFHSLMVLEKIIGNSAFLLILKDKLTYDSYSPDTFLE
                     MDLKQKSQDSLPQKNIIRLSAEPRYLVVYNPLEQDRISLVSVYVSSPTVQVFSASGKP
                     VEVQVSAVWDTANTISETAYEISFRAHIPPLGLKVYKILESASSNSHLADYVLYKNKV
                     EDSGIFTIKNMINTEEGITLENSFVLLRFDQTGLMKQMMTKEDGKHHEVNVQFSWYGT
                     TIKRDKSGAYLFLPDGNAKPYVYTTPPFVRVTHGRIYSEVTCFFDHVTHRVRLYHIQG
                     IEGQSVEVSNIVDIRKVYNREIAMKISSDIKSQNRFYTDLNGYQIQPRMTLSKLPLQA
                     NVYPMTTMAYIQDAKHRLTLLSAQSLGVSSLNSGQIEVIMDRRLMQDDNRGLEQGIQD
                     NKITANLFRILLEKRSAVNTEEEKKSVSYPSLLSHITSSLMNHPVIPMANKFSSPTLE
                     LQGEFSPLQSSLPCDIHLVNLRTIQSKVGNGHSNEAALILHRKGFDCRFSSKGTGLFC
                     STTQGKILVQKLLNKFIVESLTPSSLSLMHSPPGTQNISEINLSPMEISTFRIQLR"
     exon            627..881
                     /gene="MAN2A1"
                     /gene_synonym="AMan II; GOLIM7; MANA2; MANII"
                     /inference="alignment:Splign:2.1.0"
     exon            882..1026
                     /gene="MAN2A1"
                     /gene_synonym="AMan II; GOLIM7; MANA2; MANII"
                     /inference="alignment:Splign:2.1.0"
     exon            1027..1198
                     /gene="MAN2A1"
                     /gene_synonym="AMan II; GOLIM7; MANA2; MANII"
                     /inference="alignment:Splign:2.1.0"
     exon            1199..1326
                     /gene="MAN2A1"
                     /gene_synonym="AMan II; GOLIM7; MANA2; MANII"
                     /inference="alignment:Splign:2.1.0"
     exon            1327..1500
                     /gene="MAN2A1"
                     /gene_synonym="AMan II; GOLIM7; MANA2; MANII"
                     /inference="alignment:Splign:2.1.0"
     exon            1501..1687
                     /gene="MAN2A1"
                     /gene_synonym="AMan II; GOLIM7; MANA2; MANII"
                     /inference="alignment:Splign:2.1.0"
     exon            1688..1865
                     /gene="MAN2A1"
                     /gene_synonym="AMan II; GOLIM7; MANA2; MANII"
                     /inference="alignment:Splign:2.1.0"
     exon            1866..2068
                     /gene="MAN2A1"
                     /gene_synonym="AMan II; GOLIM7; MANA2; MANII"
                     /inference="alignment:Splign:2.1.0"
     exon            2069..2251
                     /gene="MAN2A1"
                     /gene_synonym="AMan II; GOLIM7; MANA2; MANII"
                     /inference="alignment:Splign:2.1.0"
     exon            2252..2366
                     /gene="MAN2A1"
                     /gene_synonym="AMan II; GOLIM7; MANA2; MANII"
                     /inference="alignment:Splign:2.1.0"
     exon            2367..2434
                     /gene="MAN2A1"
                     /gene_synonym="AMan II; GOLIM7; MANA2; MANII"
                     /inference="alignment:Splign:2.1.0"
     exon            2435..2600
                     /gene="MAN2A1"
                     /gene_synonym="AMan II; GOLIM7; MANA2; MANII"
                     /inference="alignment:Splign:2.1.0"
     exon            2601..2819
                     /gene="MAN2A1"
                     /gene_synonym="AMan II; GOLIM7; MANA2; MANII"
                     /inference="alignment:Splign:2.1.0"
     exon            2820..2942
                     /gene="MAN2A1"
                     /gene_synonym="AMan II; GOLIM7; MANA2; MANII"
                     /inference="alignment:Splign:2.1.0"
     exon            2943..3057
                     /gene="MAN2A1"
                     /gene_synonym="AMan II; GOLIM7; MANA2; MANII"
                     /inference="alignment:Splign:2.1.0"
     exon            3058..3191
                     /gene="MAN2A1"
                     /gene_synonym="AMan II; GOLIM7; MANA2; MANII"
                     /inference="alignment:Splign:2.1.0"
     exon            3192..3333
                     /gene="MAN2A1"
                     /gene_synonym="AMan II; GOLIM7; MANA2; MANII"
                     /inference="alignment:Splign:2.1.0"
     exon            3334..3467
                     /gene="MAN2A1"
                     /gene_synonym="AMan II; GOLIM7; MANA2; MANII"
                     /inference="alignment:Splign:2.1.0"
     exon            3468..3662
                     /gene="MAN2A1"
                     /gene_synonym="AMan II; GOLIM7; MANA2; MANII"
                     /inference="alignment:Splign:2.1.0"
     exon            3663..3773
                     /gene="MAN2A1"
                     /gene_synonym="AMan II; GOLIM7; MANA2; MANII"
                     /inference="alignment:Splign:2.1.0"
     exon            3774..6553
                     /gene="MAN2A1"
                     /gene_synonym="AMan II; GOLIM7; MANA2; MANII"
                     /inference="alignment:Splign:2.1.0"
     regulatory      6530..6535
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MAN2A1"
                     /gene_synonym="AMan II; GOLIM7; MANA2; MANII"
                     /note="hexamer: AATAAA"
     polyA_site      6553
                     /gene="MAN2A1"
                     /gene_synonym="AMan II; GOLIM7; MANA2; MANII"
                     /note="major polyA site"
ORIGIN      
        1 cctttcccct ccgcttctct gacctagctg cgcggccccg gcccgggagc tgccgaaccc
       61 gcgcctcccc tgggtgagga ggacacgcct gccctcgtcg agaaaacttt tcctgccgac
      121 tcagttgggg cggcggtggc aggaagtgcg ggcagcgacc tctcctccgc ctgccccgcg
      181 cgccctgccg gaggtcggcg ctgagcttgc gatcaagttt gtgggggccc cccttcccag
      241 ttgccggcga gtctcgcctc gagaggggcg cccgaccccg gggagggcgg caggccaggg
      301 cgaaggccaa gggcgtgtgg tggcgccgga gactaggtgc ggagcaaggc ggggactcgc
      361 acccgcatcc gagagcgcgg aggtcgcgca gcccgggaga agggagcctc cggcggctgc
      421 ttcctagagt ccacagtgcg ctgtctcctt tggctgagga gagtgtcctg gccccgagtc
      481 tatcgaggaa aatgaagtta agccgccagt tcaccgtgtt cggcagtgcg atcttctgtg
      541 tggtgatttt ctcgctctac ctgatgctgg accggggtca cttagactac cccaggaacc
      601 cgcgccgcga gggctccttc cctcagggcc agctctcaat gttgcaagaa aaaatagacc
      661 atttggagcg tttgctagct gagaataatg agatcatctc aaatattaga gactcagtca
      721 tcaatttgag tgagtctgtg gaggatggtc cgaaaagttc acaaagcaat ttcagccaag
      781 gtgctggctc acatcttctg ccctcacaat tatccctctc agttgacact gcagactgtc
      841 tgtttgcttc acaaagtgga agtcacaatt cagatgtgca gatgttggat gtttacagtc
      901 taatttcttt tgacaatcca gatggtggag tttggaagca aggatttgac attacttatg
      961 aatctaatga atgggacact gaaccccttc aagtctttgt ggtgcctcat tcccataacg
     1021 acccaggttg gttgaagact ttcaatgact actttagaga caagactcag tatattttta
     1081 ataacatggt cctaaagctg aaagaagact cacggaggaa gtttatttgg tctgagatct
     1141 cttacctttc aaagtggtgg gatattatag atattcagaa gaaggatgct gttaaaagtt
     1201 taatagaaaa tggtcagctt gaaattgtga caggtggctg ggttatgcct gatgaagcta
     1261 ctccacatta ttttgcctta attgatcaac taattgaagg acatcagtgg ctggaaaata
     1321 atataggagt gaaacctcgg tccggctggg ctattgatcc ctttggacac tcaccaacaa
     1381 tggcttatct tctaaaccgt gctggacttt ctcacatgct tatccagaga gttcattatg
     1441 cagttaaaaa acactttgca ctgcataaaa cattggagtt tttttggaga cagaattggg
     1501 atctgggatc tgtcacagat attttatgcc acatgatgcc cttctacagc tatgacatcc
     1561 ctcacacttg tggacctgat cctaaaatat gctgccagtt tgattttaaa cgtcttcctg
     1621 gaggcagatt tggttgtccc tggggagtcc ccccagaaac aatacatcct ggaaatgtcc
     1681 aaagcagggc tcggatgcta ctagatcagt accgaaagaa gtcaaagctt tttcgtacca
     1741 aagttctcct ggctccacta ggagatgatt tccgctactg tgaatacacg gaatgggatt
     1801 tacagtttaa gaattatcag cagctttttg attatatgaa ttctcagtcc aagtttaaag
     1861 ttaagataca gtttggaact ttatcagatt tttttgatgc gctggataaa gcagatgaaa
     1921 ctcagagaga caagggccaa tcgatgttcc ctgttttaag tggagatttt ttcacttatg
     1981 ccgatcgaga tgatcattac tggagtggct attttacatc cagacccttt tacaaacgaa
     2041 tggacagaat catggaatct catttaaggg ctgctgaaat tctttactat ttcgccctga
     2101 gacaagctca caaatacaag ataaataaat ttctctcatc atcactttac acggcactga
     2161 cagaagccag aaggaatttg ggactgtttc aacatcatga tgctatcaca ggaactgcaa
     2221 aagactgggt ggttgtggat tatggtacca gactttttca ttcgttaatg gttttggaga
     2281 agataattgg aaattctgca tttcttctta ttttgaagga caaactcaca tacgactctt
     2341 actctcctga taccttcctg gagatggatt tgaaacaaaa atcacaagat tctctgccac
     2401 aaaaaaatat aataaggctg agtgcggagc caaggtacct tgtggtctat aatcctttag
     2461 aacaagaccg aatctcgttg gtctcagtct atgtgagttc cccgacagtg caagtgttct
     2521 ctgcttcagg aaaacctgtg gaagttcaag tcagcgcagt ttgggataca gcaaatacta
     2581 tttcagaaac agcctatgag atctcttttc gagcacatat accgccattg ggactgaaag
     2641 tgtataagat tttggaatca gcaagttcaa attcacattt agctgattat gtcttgtata
     2701 agaataaagt agaagatagc ggaattttca ccataaagaa tatgataaat actgaagaag
     2761 gtataacact agagaactcc tttgttttac ttcggtttga tcaaactgga cttatgaagc
     2821 aaatgatgac taaagaagat ggtaaacacc atgaagtaaa tgtgcaattt tcatggtatg
     2881 gaaccacaat taaaagagac aaaagtggtg cctacctctt cttacctgat ggtaatgcca
     2941 agccttatgt ttacacaaca ccgccctttg tcagagtgac acatggaagg atttattcgg
     3001 aagtgacttg cttttttgac catgttactc atagagtccg actataccac atacagggaa
     3061 tagaaggaca gtctgtggaa gtttccaata ttgtggacat ccgaaaagta tataaccgtg
     3121 agattgcaat gaaaatttct tctgatataa aaagccaaaa tagattttat actgacctaa
     3181 atgggtacca gattcaacct agaatgacac tgagcaaatt gcctcttcaa gcaaatgtct
     3241 atcccatgac cacaatggcc tatatccagg atgccaaaca tcgtttgaca ctgctctctg
     3301 ctcagtcatt aggggtttcg agtttgaata gtggtcagat tgaagttatc atggatcgaa
     3361 gactcatgca agatgataat cgtggccttg agcaaggtat ccaggataac aagattacag
     3421 ctaatctatt tcgaatacta ctagaaaaaa gaagtgctgt taatacggaa gaagaaaaga
     3481 agtcggtcag ttatccttct ctccttagcc acataacttc ttctctcatg aatcatccag
     3541 tcattccaat ggcaaataag ttctcctcac ctacccttga gctgcaaggt gaattctctc
     3601 cattacagtc atctttgcct tgtgacattc atctggttaa tttgagaaca atacagtcaa
     3661 aggtgggcaa tgggcactcc aatgaggcag ccttgatcct ccacagaaaa gggtttgatt
     3721 gtcggttctc tagcaaaggc acagggctgt tttgttctac tactcaggga aagatattgg
     3781 tacagaaact tttaaacaag tttattgtcg aaagtctcac accttcatca ctatccttga
     3841 tgcattcacc tcccggcact cagaatataa gtgagatcaa cttgagtcca atggaaatca
     3901 gcacattccg aatccagttg aggtgaacct gactttcaca tttggattga gaatcattgg
     3961 cttttatacc tttcttggtt tgacgtgcaa taaagaagca cattatttta gcttctggct
     4021 actgtgagaa catgaattct gtgattctgt gggttttttc ttttttcttt taccagtaca
     4081 gtaagaaaaa aaaaaaaaaa aaaaaagcca tgctatcaat caagattctt tttttttaaa
     4141 ctttctccca tgaactacca ccatcagtat gaattgatgc aacaaatgaa gaaatattta
     4201 aagacagcct ctcaacagat tgtatctcag gttaaatgct aactaattat gtctgtgttg
     4261 ggggttgcga agagattctt aaaagtatct gtgtgttgat catcagtttt acaaaaacac
     4321 ctatttggct gaaatggaat aaaatgtttg tgggtaaaag ctaatggcca aaatggttgc
     4381 aatcattcat actagttaga aaaattatgt gttgaaataa gtggaaaagt gcaatccatc
     4441 cacccttatg attaacgtag atgattttta tacctttttc tgatgtacct cttgaccttc
     4501 tccttccctt cctacccttt ctaagtattt ccagaaatac ctgattttga atcattcaac
     4561 agtagaaaaa gaggcatatt ttcattactt gacaatgtgg gatgggtgca atttattcca
     4621 tcttcactaa aatagaagca attccatagg taccataaac ctattttagg taccacaagg
     4681 tgtcttttta cacagctcat ttgaatacag gtgttctgag aaggggtttc tattttaaaa
     4741 ttaccatatc aaaataaatg tgccttattt ttttataagt cttgttaaat cagtgtccat
     4801 attactgttt ggggaagggg gaatgttgtg gggtctggga gagggtgggt actttctatg
     4861 acacataaat tgtgtaattt ttgcctgaca atgctggcca cattctgatc tgtttcatta
     4921 aatttgtggt gatgttactc taaacatttt gactatttga atgtactgag atgtcagaaa
     4981 acaaaacaag gaaggaaaat attgttaatt aaaatgtgct gctgccaagg aaactgcaac
     5041 ttgaagcaag gattttgtaa aatgcaaaat ccagctactg tttccatttc acagtagtta
     5101 actatattaa agagagaatg ctttaaaatt gatcttgttt tgaaacccac ttttatgtag
     5161 ctcatcatgg tttatcttac taaggaatat gtttgttcat tcagttctca acttttgtat
     5221 gtgctaacct taaagtgaag ttctgagccc gtgtgccatt acagtgcttt taataaaatt
     5281 tatttgggat tattgtttcc ttaacattaa aataatagcg acatttagac tatgcaattt
     5341 tagcatagaa aggagtcttt gagtatgtac agttttgaaa attctctttg agataattga
     5401 tttcatattc tgtggctttc aacctccatt tacctcttgt cattccaaca tctttataga
     5461 gaaataaaaa cccaatttct ctttcaccat ttagtttgat tatcatctgg attttcactc
     5521 aagatgcagc tcctaagatt attgttatgt taaattcata aactccttca cctttaataa
     5581 ttaaggaaac aataccagtg ttgataaaga tattacaagg ggtaatttca tgcaataaac
     5641 atgtaccgta agttttcttc cacatatttt gggaaaaaac taaaaaaaga aaaaggactt
     5701 cctttttgtg gacatctaca gatgttaggg ttgccagaag caaatcccag gaatgagatc
     5761 agtattttca ttgcatctta aatgtataac cttcctgtgg gagttcagtt tgtctgtggt
     5821 taagtgggtg tgcttaatca ttctcgaaat tgtgatcaga tgaaataaaa aaaaaatctt
     5881 gatgcaataa cagtggtttt gccacttctg gttgtttgcg atggatctgt cccatgtcag
     5941 tctggggttt tattcagctt gtgttgctac cagcagttca caggtaaagc agaaattctc
     6001 tttaaccagc aagtttctgc tttttaaggt tacttttaga ataaatcatc agggaaacag
     6061 agaggatgct ttgctttggg ttgtagtcaa aaactgatta aataatttaa tgtctctggc
     6121 acacactaaa aaccatacac ttcagttgtg atctcagtgg catatttatt tggttaggtt
     6181 tcgttacatt tattattaca gatgttcagt tgaccaagta gttcagtgtt ttctttcctt
     6241 tttttggaaa ttttagtttg agtttgtgac tgcagtgttc aagaactcag catccttgtt
     6301 ttctacaaat actgattaaa ataaaatgct gtaaaatgtg atgtaaaaca ttatcatgat
     6361 cttcccatgc ctttgttgta cttgtgccga agtgttttga tattcctttg tctggaagaa
     6421 aatgtttgct ttcattttga tcattttgtt caccttggaa tcaacaggtt ttgatatttt
     6481 ctcttggaag attttatatc tttttgggaa tatgtaatat aagatctcta ataaaagata
     6541 atcttatcat gta
//
