LOCUS       NM_018048               2606 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens mago homolog B, exon junction complex subunit
            (MAGOHB), transcript variant 1, mRNA.
ACCESSION   NM_018048
VERSION     NM_018048.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2606)
  AUTHORS   Soederberg,A., Meissgeier,T., Bosserhoff,A.K. and Linck-Paulus,L.
  TITLE     MAGOH and MAGOHB Knockdown in Melanoma Cells Decreases
            Nonsense-Mediated Decay Activity and Promotes Apoptosis via
            Upregulation of GADD45A
  JOURNAL   Cells 11 (23), 3859 (2022)
   PUBMED   36497117
  REMARK    GeneRIF: MAGOH and MAGOHB Knockdown in Melanoma Cells Decreases
            Nonsense-Mediated Decay Activity and Promotes Apoptosis via
            Upregulation of GADD45A.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2606)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   3  (bases 1 to 2606)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   4  (bases 1 to 2606)
  AUTHORS   Zhan,X., Yan,C., Zhang,X., Lei,J. and Shi,Y.
  TITLE     Structure of a human catalytic step I spliceosome
  JOURNAL   Science 359 (6375), 537-545 (2018)
   PUBMED   29301961
REFERENCE   5  (bases 1 to 2606)
  AUTHORS   Zhang,X., Yan,C., Hang,J., Finci,L.I., Lei,J. and Shi,Y.
  TITLE     An Atomic Structure of the Human Spliceosome
  JOURNAL   Cell 169 (5), 918-929 (2017)
   PUBMED   28502770
REFERENCE   6  (bases 1 to 2606)
  AUTHORS   Gong,P., Zhao,M. and He,C.
  TITLE     Slow co-evolution of the MAGO and Y14 protein families is required
            for the maintenance of their obligate heterodimerization mode
  JOURNAL   PLoS One 9 (1), e84842 (2014)
   PUBMED   24416299
  REMARK    Publication Status: Online-Only
REFERENCE   7  (bases 1 to 2606)
  AUTHORS   Singh,K.K., Wachsmuth,L., Kulozik,A.E. and Gehring,N.H.
  TITLE     Two mammalian MAGOH genes contribute to exon junction complex
            composition and nonsense-mediated decay
  JOURNAL   RNA Biol 10 (8), 1291-1298 (2013)
   PUBMED   23917022
  REMARK    GeneRIF: findings show 2 genes MAGOH and MAGOHB are expressed in
            mammals; in macrophages, expression of MAGOHB but not MAGOH mRNA
            increases after LPS stimulation; both MAGOH proteins interact with
            other exon junction complex (EJC) components, incorporate into
            mRNA-bound EJCs and activate nonsense-mediated decay
REFERENCE   8  (bases 1 to 2606)
  AUTHORS   Baltz,A.G., Munschauer,M., Schwanhausser,B., Vasile,A.,
            Murakawa,Y., Schueler,M., Youngs,N., Penfold-Brown,D., Drew,K.,
            Milek,M., Wyler,E., Bonneau,R., Selbach,M., Dieterich,C. and
            Landthaler,M.
  TITLE     The mRNA-bound proteome and its global occupancy profile on
            protein-coding transcripts
  JOURNAL   Mol Cell 46 (5), 674-690 (2012)
   PUBMED   22681889
REFERENCE   9  (bases 1 to 2606)
  AUTHORS   Bono,F. and Gehring,N.H.
  TITLE     Assembly, disassembly and recycling: the dynamics of exon junction
            complexes
  JOURNAL   RNA Biol 8 (1), 24-29 (2011)
   PUBMED   21289489
  REMARK    Review article
REFERENCE   10 (bases 1 to 2606)
  AUTHORS   Le Hir,H., Gatfield,D., Izaurralde,E. and Moore,M.J.
  TITLE     The exon-exon junction complex provides a binding platform for
            factors involved in mRNA export and nonsense-mediated mRNA decay
  JOURNAL   EMBO J 20 (17), 4987-4997 (2001)
   PUBMED   11532962
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BG533740.1, CD357202.1, AF165518.1 and AC021049.12.
            
            On Nov 22, 2018 this sequence version replaced NM_018048.4.
            
            Transcript Variant: This variant (1) encodes the longer isoform
            (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: DA500103.1, AK022720.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000320756.7/ ENSP00000319240.2
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-214               BG533740.1         2-215
            215-230             CD357202.1         218-233
            231-694             AF165518.1         227-690
            695-2606            AC021049.12        143467-145378
FEATURES             Location/Qualifiers
     source          1..2606
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="12"
                     /map="12p13.2"
     gene            1..2606
                     /gene="MAGOHB"
                     /gene_synonym="mago; magoh; MGN2"
                     /note="mago homolog B, exon junction complex subunit"
                     /db_xref="GeneID:55110"
                     /db_xref="HGNC:HGNC:25504"
                     /db_xref="MIM:619552"
     exon            1..171
                     /gene="MAGOHB"
                     /gene_synonym="mago; magoh; MGN2"
                     /inference="alignment:Splign:2.1.0"
     CDS             78..524
                     /gene="MAGOHB"
                     /gene_synonym="mago; magoh; MGN2"
                     /note="isoform 1 is encoded by transcript variant 1;
                     protein mago nashi homolog 2; mago-nashi homolog B; mago
                     homolog B, exon junction complex core component"
                     /codon_start=1
                     /product="protein mago nashi homolog 2 isoform 1"
                     /protein_id="NP_060518.1"
                     /db_xref="CCDS:CCDS8628.1"
                     /db_xref="GeneID:55110"
                     /db_xref="HGNC:HGNC:25504"
                     /db_xref="MIM:619552"
                     /translation="MAVASDFYLRYYVGHKGKFGHEFLEFEFRPDGKLRYANNSNYKN
                     DVMIRKEAYVHKSVMEELKRIIDDSEITKEDDALWPPPDRVGRQELEIVIGDEHISFT
                     TSKIGSLIDVNQSKDPEGLRVFYYLVQDLKCLVFSLIGLHFKIKPI"
     exon            172..230
                     /gene="MAGOHB"
                     /gene_synonym="mago; magoh; MGN2"
                     /inference="alignment:Splign:2.1.0"
     exon            231..341
                     /gene="MAGOHB"
                     /gene_synonym="mago; magoh; MGN2"
                     /inference="alignment:Splign:2.1.0"
     exon            342..424
                     /gene="MAGOHB"
                     /gene_synonym="mago; magoh; MGN2"
                     /inference="alignment:Splign:2.1.0"
     exon            425..2606
                     /gene="MAGOHB"
                     /gene_synonym="mago; magoh; MGN2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      510..515
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MAGOHB"
                     /gene_synonym="mago; magoh; MGN2"
                     /note="hexamer: ATTAAA"
     polyA_site      530
                     /gene="MAGOHB"
                     /gene_synonym="mago; magoh; MGN2"
                     /note="major polyA site"
     regulatory      733..738
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MAGOHB"
                     /gene_synonym="mago; magoh; MGN2"
                     /note="hexamer: AATGAA"
     regulatory      766..771
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MAGOHB"
                     /gene_synonym="mago; magoh; MGN2"
                     /note="hexamer: AATAAA"
     polyA_site      772
                     /gene="MAGOHB"
                     /gene_synonym="mago; magoh; MGN2"
     polyA_site      786
                     /gene="MAGOHB"
                     /gene_synonym="mago; magoh; MGN2"
                     /note="major polyA site"
     regulatory      2584..2589
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MAGOHB"
                     /gene_synonym="mago; magoh; MGN2"
                     /note="hexamer: AATAAA"
     polyA_site      2606
                     /gene="MAGOHB"
                     /gene_synonym="mago; magoh; MGN2"
ORIGIN      
        1 attccgcgcg atgacgtcac tgcaaggcgc cgggggacac gttggctgcg ttttcggcgg
       61 gcttcccggg tacaaaaatg gctgtggcta gcgatttcta cctgcgctac tacgtagggc
      121 acaagggcaa gtttgggcac gagtttctgg agttcgaatt tcggccggac ggaaagctta
      181 gatatgccaa caacagcaat tacaaaaatg atgtcatgat cagaaaagag gcttatgtgc
      241 acaagagtgt aatggaagaa ctgaagagaa ttattgatga cagtgaaatt acaaaagaag
      301 atgatgcttt gtggcctccc cctgataggg ttggccgaca ggagcttgaa attgtaattg
      361 gagatgagca catatctttt accacatcaa aaataggttc tcttattgat gtaaatcagt
      421 caaaggatcc tgaaggcctt cgagtatttt actatttggt acaagacttg aaatgtttag
      481 ttttcagtct tattggatta cacttcaaga ttaaaccaat ttaaattgta tgttttcagg
      541 ctgtttgtat atttaattaa gggatgggag gggttatttg tcatttacag tattggggtt
      601 tttatgaatg tgaagcaaac aaaaaaaatt tgtatgtaaa ctgaaaataa gaaaatacat
      661 tagcaagctt aatggttatc cttacttgag tccatatggg ttggacagtc cccacacaca
      721 ttaaattctg taaatgaaag ccaccttttg ttaaaaattt gctctaataa aacataccaa
      781 atcctggttg cagagtagtt ttttgttttt tccaggaggc tatgtctcta attcacttta
      841 gagataataa gaaattgttc tggtagatat atcctgtgac agaagatact ttaggtggaa
      901 ctatgtagcc agattcccat ccatgaaagg caagtgtaga ttgtccctta tttccttcat
      961 acatgattgg atttaatttt ggggggctta tacaaggtct agtttttttt tacagttatg
     1021 acaaacccct cagggattat tcacatttaa atattttcag ttataagcag tgaggtccta
     1081 aagtgttaca agagtacagt ctaccccatg ttaggcatat ctttgattat gtctttattc
     1141 cttatttcac aatgtatttg gtgtgtaggg gaggggggag aactaaatga gttttcagct
     1201 ttataaattg ttaaacattt agacaaacat atatgtatgt atgaatgtac ataaatattt
     1261 ttaactccta ttgaccacga gtctcacttc agtttcccag ttcctttcaa cctctttctg
     1321 atagatttcc tctttcatta cttttagtaa ccatgttcct tgtttccttt tattcttcca
     1381 tctgaagccc cactcttaaa aagttgcact gttccagtag ttataatcca cttgccctag
     1441 gaacaagtta gcactgaatt ttgggtggaa taattagttt ctgaaggctt gccaggactc
     1501 ctgagcaggt aggctctaga gtcgggcagt ccaataactt ttttgcaata atggaaacgt
     1561 tctatgtgca gtccaatagg gtagcaactg gccaaatgag gcctactgat tacttgaggt
     1621 gtgccttgta taactgaatt tatggtgcta tttaaacaat ttttttctaa cgtgaaaagg
     1681 ataaaacatt aaaaactctt gagaactata aagtgaacac ctatatgcct acccctacct
     1741 agattctata cttaacatct tttttactgt aatatctcta ttataataaa tcttggtttt
     1801 tcacttaact ggtgtaattg gtgccaataa actacttttt ttgtagtgct atttaatttt
     1861 gattaaattt aggtagccac gtgtctagcg gctaccgttt ggacagtata gctctagagc
     1921 atggcttggt aacctgtttg ccatggagca ctagatggtc cttttcactc ctcaaaatgc
     1981 atgcccattg tcttcaggtt tgccatggaa agtcaaatga tttccacttc attatgcaag
     2041 tacgctatca tcttcaggtc ttttgtatgt aaaatgtttc tgttccagtt gtagaccttg
     2101 atgattgtgc agtatgaaat cgtattgtaa ttttcttgca tttagatgtc aacctcagaa
     2161 acaggaacaa tcgtcttttg aacttccagt aggcccacag ttgttggttg ttcctcaaaa
     2221 caggttgtgg ctcctgttga ataagatgat ccattaaaaa ctgaacaagg ttgaggagaa
     2281 atagtgctta cgttgaaaaa tctttaagtc tttgtccccg ttctctaact tccttacgtt
     2341 ttcgtttatt tagctctatc cccactatct actagaattt ctcatattta aaccaagatg
     2401 ggagactagg tcattaggaa aatattaccg tctacaattt tcttatactt tgatctgtct
     2461 tttatttgat tgtaagttgc tgatggacag tgatcattag aaactgaatt ttgtataata
     2521 ctagttttat atgaaactag atttttattg cgctcaggtt atgttccttt tacctccttc
     2581 cttaataaag agaccacttg aaataa
//
