LOCUS       NM_001319985            2504 bp    mRNA    linear   PRI 21-SEP-2024
DEFINITION  Homo sapiens mago homolog B, exon junction complex subunit
            (MAGOHB), transcript variant 3, mRNA.
ACCESSION   NM_001319985 XM_011520718
VERSION     NM_001319985.2
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2504)
  AUTHORS   Soederberg,A., Meissgeier,T., Bosserhoff,A.K. and Linck-Paulus,L.
  TITLE     MAGOH and MAGOHB Knockdown in Melanoma Cells Decreases
            Nonsense-Mediated Decay Activity and Promotes Apoptosis via
            Upregulation of GADD45A
  JOURNAL   Cells 11 (23), 3859 (2022)
   PUBMED   36497117
  REMARK    GeneRIF: MAGOH and MAGOHB Knockdown in Melanoma Cells Decreases
            Nonsense-Mediated Decay Activity and Promotes Apoptosis via
            Upregulation of GADD45A.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2504)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   3  (bases 1 to 2504)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   4  (bases 1 to 2504)
  AUTHORS   Zhan,X., Yan,C., Zhang,X., Lei,J. and Shi,Y.
  TITLE     Structure of a human catalytic step I spliceosome
  JOURNAL   Science 359 (6375), 537-545 (2018)
   PUBMED   29301961
REFERENCE   5  (bases 1 to 2504)
  AUTHORS   Zhang,X., Yan,C., Hang,J., Finci,L.I., Lei,J. and Shi,Y.
  TITLE     An Atomic Structure of the Human Spliceosome
  JOURNAL   Cell 169 (5), 918-929 (2017)
   PUBMED   28502770
REFERENCE   6  (bases 1 to 2504)
  AUTHORS   Gong,P., Zhao,M. and He,C.
  TITLE     Slow co-evolution of the MAGO and Y14 protein families is required
            for the maintenance of their obligate heterodimerization mode
  JOURNAL   PLoS One 9 (1), e84842 (2014)
   PUBMED   24416299
  REMARK    Publication Status: Online-Only
REFERENCE   7  (bases 1 to 2504)
  AUTHORS   Singh,K.K., Wachsmuth,L., Kulozik,A.E. and Gehring,N.H.
  TITLE     Two mammalian MAGOH genes contribute to exon junction complex
            composition and nonsense-mediated decay
  JOURNAL   RNA Biol 10 (8), 1291-1298 (2013)
   PUBMED   23917022
  REMARK    GeneRIF: findings show 2 genes MAGOH and MAGOHB are expressed in
            mammals; in macrophages, expression of MAGOHB but not MAGOH mRNA
            increases after LPS stimulation; both MAGOH proteins interact with
            other exon junction complex (EJC) components, incorporate into
            mRNA-bound EJCs and activate nonsense-mediated decay
REFERENCE   8  (bases 1 to 2504)
  AUTHORS   Baltz,A.G., Munschauer,M., Schwanhausser,B., Vasile,A.,
            Murakawa,Y., Schueler,M., Youngs,N., Penfold-Brown,D., Drew,K.,
            Milek,M., Wyler,E., Bonneau,R., Selbach,M., Dieterich,C. and
            Landthaler,M.
  TITLE     The mRNA-bound proteome and its global occupancy profile on
            protein-coding transcripts
  JOURNAL   Mol Cell 46 (5), 674-690 (2012)
   PUBMED   22681889
REFERENCE   9  (bases 1 to 2504)
  AUTHORS   Bono,F. and Gehring,N.H.
  TITLE     Assembly, disassembly and recycling: the dynamics of exon junction
            complexes
  JOURNAL   RNA Biol 8 (1), 24-29 (2011)
   PUBMED   21289489
  REMARK    Review article
REFERENCE   10 (bases 1 to 2504)
  AUTHORS   Le Hir,H., Gatfield,D., Izaurralde,E. and Moore,M.J.
  TITLE     The exon-exon junction complex provides a binding platform for
            factors involved in mRNA export and nonsense-mediated mRNA decay
  JOURNAL   EMBO J 20 (17), 4987-4997 (2001)
   PUBMED   11532962
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BG533740.1, BF667243.1, CD357202.1, AF165518.1 and AC021049.12.
            
            On Aug 13, 2020 this sequence version replaced NM_001319985.1.
            
            Transcript Variant: This variant (3) uses an alternate splice site
            in the 5' region and initiates translation at a downstream start
            codon compared to variant 1. The encoded isoform (2) has a shorter
            N-terminus compared to isoform 1. Variants 2 and 3 encode the same
            isoform (2).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BF667243.1, SRR18074967.3454188.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1968832
                                           [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-43                BG533740.1         2-44
            44-112              BF667243.1         26-94
            113-128             CD357202.1         218-233
            129-592             AF165518.1         227-690
            593-2504            AC021049.12        143467-145378
FEATURES             Location/Qualifiers
     source          1..2504
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="12"
                     /map="12p13.2"
     gene            1..2504
                     /gene="MAGOHB"
                     /gene_synonym="mago; magoh; MGN2"
                     /note="mago homolog B, exon junction complex subunit"
                     /db_xref="GeneID:55110"
                     /db_xref="HGNC:HGNC:25504"
                     /db_xref="MIM:619552"
     exon            1..69
                     /gene="MAGOHB"
                     /gene_synonym="mago; magoh; MGN2"
                     /inference="alignment:Splign:2.1.0"
     exon            70..128
                     /gene="MAGOHB"
                     /gene_synonym="mago; magoh; MGN2"
                     /inference="alignment:Splign:2.1.0"
     CDS             114..422
                     /gene="MAGOHB"
                     /gene_synonym="mago; magoh; MGN2"
                     /note="isoform 2 is encoded by transcript variant 3;
                     protein mago nashi homolog 2; mago-nashi homolog B; mago
                     homolog B, exon junction complex core component"
                     /codon_start=1
                     /product="protein mago nashi homolog 2 isoform 2"
                     /protein_id="NP_001306914.1"
                     /db_xref="CCDS:CCDS76532.1"
                     /db_xref="GeneID:55110"
                     /db_xref="HGNC:HGNC:25504"
                     /db_xref="MIM:619552"
                     /translation="MIRKEAYVHKSVMEELKRIIDDSEITKEDDALWPPPDRVGRQEL
                     EIVIGDEHISFTTSKIGSLIDVNQSKDPEGLRVFYYLVQDLKCLVFSLIGLHFKIKPI
                     "
     exon            129..239
                     /gene="MAGOHB"
                     /gene_synonym="mago; magoh; MGN2"
                     /inference="alignment:Splign:2.1.0"
     exon            240..322
                     /gene="MAGOHB"
                     /gene_synonym="mago; magoh; MGN2"
                     /inference="alignment:Splign:2.1.0"
     exon            323..2504
                     /gene="MAGOHB"
                     /gene_synonym="mago; magoh; MGN2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      408..413
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MAGOHB"
                     /gene_synonym="mago; magoh; MGN2"
                     /note="hexamer: ATTAAA"
     polyA_site      428
                     /gene="MAGOHB"
                     /gene_synonym="mago; magoh; MGN2"
                     /note="major polyA site"
     regulatory      631..636
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MAGOHB"
                     /gene_synonym="mago; magoh; MGN2"
                     /note="hexamer: AATGAA"
     regulatory      664..669
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MAGOHB"
                     /gene_synonym="mago; magoh; MGN2"
                     /note="hexamer: AATAAA"
     polyA_site      670
                     /gene="MAGOHB"
                     /gene_synonym="mago; magoh; MGN2"
     polyA_site      684
                     /gene="MAGOHB"
                     /gene_synonym="mago; magoh; MGN2"
                     /note="major polyA site"
     regulatory      2482..2487
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MAGOHB"
                     /gene_synonym="mago; magoh; MGN2"
                     /note="hexamer: AATAAA"
     polyA_site      2504
                     /gene="MAGOHB"
                     /gene_synonym="mago; magoh; MGN2"
ORIGIN      
        1 attccgcgcg atgacgtcac tgcaaggcgc cgggggacac gttggctgcg ttttcggcgg
       61 gcttcccggg aaagcttaga tatgccaaca acagcaatta caaaaatgat gtcatgatca
      121 gaaaagaggc ttatgtgcac aagagtgtaa tggaagaact gaagagaatt attgatgaca
      181 gtgaaattac aaaagaagat gatgctttgt ggcctccccc tgatagggtt ggccgacagg
      241 agcttgaaat tgtaattgga gatgagcaca tatcttttac cacatcaaaa ataggttctc
      301 ttattgatgt aaatcagtca aaggatcctg aaggccttcg agtattttac tatttggtac
      361 aagacttgaa atgtttagtt ttcagtctta ttggattaca cttcaagatt aaaccaattt
      421 aaattgtatg ttttcaggct gtttgtatat ttaattaagg gatgggaggg gttatttgtc
      481 atttacagta ttggggtttt tatgaatgtg aagcaaacaa aaaaaatttg tatgtaaact
      541 gaaaataaga aaatacatta gcaagcttaa tggttatcct tacttgagtc catatgggtt
      601 ggacagtccc cacacacatt aaattctgta aatgaaagcc accttttgtt aaaaatttgc
      661 tctaataaaa cataccaaat cctggttgca gagtagtttt ttgttttttc caggaggcta
      721 tgtctctaat tcactttaga gataataaga aattgttctg gtagatatat cctgtgacag
      781 aagatacttt aggtggaact atgtagccag attcccatcc atgaaaggca agtgtagatt
      841 gtcccttatt tccttcatac atgattggat ttaattttgg ggggcttata caaggtctag
      901 ttttttttta cagttatgac aaacccctca gggattattc acatttaaat attttcagtt
      961 ataagcagtg aggtcctaaa gtgttacaag agtacagtct accccatgtt aggcatatct
     1021 ttgattatgt ctttattcct tatttcacaa tgtatttggt gtgtagggga ggggggagaa
     1081 ctaaatgagt tttcagcttt ataaattgtt aaacatttag acaaacatat atgtatgtat
     1141 gaatgtacat aaatattttt aactcctatt gaccacgagt ctcacttcag tttcccagtt
     1201 cctttcaacc tctttctgat agatttcctc tttcattact tttagtaacc atgttccttg
     1261 tttcctttta ttcttccatc tgaagcccca ctcttaaaaa gttgcactgt tccagtagtt
     1321 ataatccact tgccctagga acaagttagc actgaatttt gggtggaata attagtttct
     1381 gaaggcttgc caggactcct gagcaggtag gctctagagt cgggcagtcc aataactttt
     1441 ttgcaataat ggaaacgttc tatgtgcagt ccaatagggt agcaactggc caaatgaggc
     1501 ctactgatta cttgaggtgt gccttgtata actgaattta tggtgctatt taaacaattt
     1561 ttttctaacg tgaaaaggat aaaacattaa aaactcttga gaactataaa gtgaacacct
     1621 atatgcctac ccctacctag attctatact taacatcttt tttactgtaa tatctctatt
     1681 ataataaatc ttggtttttc acttaactgg tgtaattggt gccaataaac tacttttttt
     1741 gtagtgctat ttaattttga ttaaatttag gtagccacgt gtctagcggc taccgtttgg
     1801 acagtatagc tctagagcat ggcttggtaa cctgtttgcc atggagcact agatggtcct
     1861 tttcactcct caaaatgcat gcccattgtc ttcaggtttg ccatggaaag tcaaatgatt
     1921 tccacttcat tatgcaagta cgctatcatc ttcaggtctt ttgtatgtaa aatgtttctg
     1981 ttccagttgt agaccttgat gattgtgcag tatgaaatcg tattgtaatt ttcttgcatt
     2041 tagatgtcaa cctcagaaac aggaacaatc gtcttttgaa cttccagtag gcccacagtt
     2101 gttggttgtt cctcaaaaca ggttgtggct cctgttgaat aagatgatcc attaaaaact
     2161 gaacaaggtt gaggagaaat agtgcttacg ttgaaaaatc tttaagtctt tgtccccgtt
     2221 ctctaacttc cttacgtttt cgtttattta gctctatccc cactatctac tagaatttct
     2281 catatttaaa ccaagatggg agactaggtc attaggaaaa tattaccgtc tacaattttc
     2341 ttatactttg atctgtcttt tatttgattg taagttgctg atggacagtg atcattagaa
     2401 actgaatttt gtataatact agttttatat gaaactagat ttttattgcg ctcaggttat
     2461 gttcctttta cctccttcct taataaagag accacttgaa ataa
//
