LOCUS       NM_001142782            6874 bp    mRNA    linear   PRI 08-JUN-2024
DEFINITION  Homo sapiens membrane associated guanylate kinase, WW and PDZ
            domain containing 3 (MAGI3), transcript variant 1, mRNA.
ACCESSION   NM_001142782
VERSION     NM_001142782.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6874)
  AUTHORS   Yang,Z., Liu,H., Song,R., Lu,W., Wang,H., Gu,S., Cao,X., Chen,Y.,
            Liang,J., Qin,Q., Yang,X., Feng,D. and He,J.
  TITLE     Reduced MAGI3 level by HPV18E6 contributes to Wnt/beta-catenin
            signaling activation and cervical cancer progression
  JOURNAL   FEBS Open Bio 11 (11), 3051-3062 (2021)
   PUBMED   34510826
  REMARK    GeneRIF: Reduced MAGI3 level by HPV18E6 contributes to
            Wnt/beta-catenin signaling activation and cervical cancer
            progression.
REFERENCE   2  (bases 1 to 6874)
  AUTHORS   Weng,Q., Chen,M., Yang,W., Li,J., Fan,K., Xu,M., Weng,W., Lv,X.,
            Fang,S., Zheng,L., Song,J., Zhao,Z., Fan,X. and Ji,J.
  TITLE     Integrated analyses identify miR-34c-3p/MAGI3 axis for the Warburg
            metabolism in hepatocellular carcinoma
  JOURNAL   FASEB J 34 (4), 5420-5434 (2020)
   PUBMED   32080912
  REMARK    GeneRIF: Integrated analyses identify miR-34c-3p/MAGI3 axis for the
            Warburg metabolism in hepatocellular carcinoma.
REFERENCE   3  (bases 1 to 6874)
  AUTHORS   Ni,T.K., Elman,J.S., Jin,D.X., Gupta,P.B. and Kuperwasser,C.
  TITLE     Premature polyadenylation of MAGI3 is associated with diminished
            N6-methyladenosine in its large internal exon
  JOURNAL   Sci Rep 8 (1), 1415 (2018)
   PUBMED   29362392
  REMARK    GeneRIF: Study shows that MAGI3 undergoes premature polyadenylation
            (pPA) at the intron immediately downstream of its large internal
            exon, which is normally highly modified by N(6)-methyladenosine
            (m(6)A). In breast cancer cells that upregulate MAGI3 (pPA) , m(6)A
            levels in the large internal exon of MAGI3 are significantly
            reduced compared to cells that do not express MAGI3 (pPA). MAGI3
            (pPA) transcripts are depleted of m(6)A.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 6874)
  AUTHORS   Noren,E., Almer,S. and Soderman,J.
  TITLE     Genetic variation and expression levels of tight junction genes
            identifies association between MAGI3 and inflammatory bowel disease
  JOURNAL   BMC Gastroenterol 17 (1), 68 (2017)
   PUBMED   28545409
  REMARK    GeneRIF: Our findings lend support to a genetic basis for
            modulation of intestinal epithelial barrier in IBD, and we have
            identified MAGI3 as a new candidate gene for IBD.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 6874)
  AUTHORS   Ni,T.K. and Kuperwasser,C.
  TITLE     Premature polyadenylation of MAGI3 produces a dominantly-acting
            oncogene in human breast cancer
  JOURNAL   Elife 5, e14730 (2016)
   PUBMED   27205883
  REMARK    GeneRIF: The altered mRNA isoform, called MAGI3(pPA), produces a
            truncated protein that acts in a dominant-negative manner to
            prevent full-length MAGI3 from interacting with the YAP
            oncoprotein, thereby relieving YAP inhibition and promoting
            malignant transformation of human mammary epithelial cells.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 6874)
  AUTHORS   Yao,R., Natsume,Y. and Noda,T.
  TITLE     MAGI-3 is involved in the regulation of the JNK signaling pathway
            as a scaffold protein for frizzled and Ltap
  JOURNAL   Oncogene 23 (36), 6023-6030 (2004)
   PUBMED   15195140
REFERENCE   7  (bases 1 to 6874)
  AUTHORS   Adamsky,K., Arnold,K., Sabanay,H. and Peles,E.
  TITLE     Junctional protein MAGI-3 interacts with receptor tyrosine
            phosphatase beta (RPTP beta) and tyrosine-phosphorylated proteins
  JOURNAL   J Cell Sci 116 (Pt 7), 1279-1289 (2003)
   PUBMED   12615970
  REMARK    GeneRIF: In epithelial cells, MAGI-3 was localized with ZO-1 and
            cingulin at tight junctions, whereas in primary cultured astrocytes
            it was found in E-cadherin-based cell-cell contacts and in focal
            adhesion sites.
REFERENCE   8  (bases 1 to 6874)
  AUTHORS   Thomas,M., Laura,R., Hepner,K., Guccione,E., Sawyers,C., Lasky,L.
            and Banks,L.
  TITLE     Oncogenic human papillomavirus E6 proteins target the MAGI-2 and
            MAGI-3 proteins for degradation
  JOURNAL   Oncogene 21 (33), 5088-5096 (2002)
   PUBMED   12140759
  REMARK    GeneRIF: Oncogenic human papillomavirus E6 proteins target the
            MAGI-2 and MAGI-3 proteins for degradation [MAGI-2, MAGI-3]
REFERENCE   9  (bases 1 to 6874)
  AUTHORS   Laura,R.P., Ross,S., Koeppen,H. and Lasky,L.A.
  TITLE     MAGI-1: a widely expressed, alternatively spliced tight junction
            protein
  JOURNAL   Exp Cell Res 275 (2), 155-170 (2002)
   PUBMED   11969287
REFERENCE   10 (bases 1 to 6874)
  AUTHORS   Wu,Y., Dowbenko,D., Spencer,S., Laura,R., Lee,J., Gu,Q. and
            Lasky,L.A.
  TITLE     Interaction of the tumor suppressor PTEN/MMAC with a PDZ domain of
            MAGI3, a novel membrane-associated guanylate kinase
  JOURNAL   J Biol Chem 275 (28), 21477-21485 (2000)
   PUBMED   10748157
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL365225.13, BC130409.1, AK311589.1, AB046854.1 and AA992863.1.
            
            On Nov 22, 2018 this sequence version replaced NM_001142782.1.
            
            Transcript Variant: This variant (1) encodes the longer protein
            (isoform 1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR18074967.2542370.1,
                                           SRR14038193.170502.1 [ECO:0000332]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000307546.14/ ENSP00000304604.9
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-338               AL365225.13        77454-77791
            339-3847            BC130409.1         1-3509
            3848-4676           AK311589.1         221-1049
            4677-5046           AK311589.1         1051-1420
            5047-6821           AB046854.1         3866-5640
            6822-6874           AA992863.1         5-57                c
FEATURES             Location/Qualifiers
     source          1..6874
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1p13.2"
     gene            1..6874
                     /gene="MAGI3"
                     /gene_synonym="dJ730K3.2; MAGI-3"
                     /note="membrane associated guanylate kinase, WW and PDZ
                     domain containing 3"
                     /db_xref="GeneID:260425"
                     /db_xref="HGNC:HGNC:29647"
                     /db_xref="MIM:615943"
     exon            1..835
                     /gene="MAGI3"
                     /gene_synonym="dJ730K3.2; MAGI-3"
                     /inference="alignment:Splign:2.1.0"
     CDS             520..4965
                     /gene="MAGI3"
                     /gene_synonym="dJ730K3.2; MAGI-3"
                     /note="isoform 1 is encoded by transcript variant 1;
                     membrane-associated guanylate kinase-related 3;
                     membrane-associated guanylate kinase, WW and PDZ
                     domain-containing protein 3; membrane-associated guanylate
                     kinase inverted 3"
                     /codon_start=1
                     /product="membrane-associated guanylate kinase, WW and PDZ
                     domain-containing protein 3 isoform 1"
                     /protein_id="NP_001136254.1"
                     /db_xref="CCDS:CCDS44196.1"
                     /db_xref="GeneID:260425"
                     /db_xref="HGNC:HGNC:29647"
                     /db_xref="MIM:615943"
                     /translation="MSKTLKKKKHWLSKVQECAVSWAGPPGDFGAEIRGGAERGEFPY
                     LGRLREEPGGGTCCVVSGKAPSPGDVLLEVNGTPVSGLTNRDTLAVIRHFREPIRLKT
                     VKPGKVINKDLRHYLSLQFQKGSIDHKLQQVIRDNLYLRTIPCTTRAPRDGEVPGVDY
                     NFISVEQFKALEESGALLESGTYDGNFYGTPKPPAEPSPFQPDPVDQVLFDNEFDAES
                     QRKRTTSVSKMERMDSSLPEEEEDEDKEAINGSGNAENRERHSESSDWMKTVPSYNQT
                     NSSMDFRNYMMRDETLEPLPKNWEMAYTDTGMIYFIDHNTKTTTWLDPRLCKKAKAPE
                     DCEDGELPYGWEKIEDPQYGTYYVDHLNQKTQFENPVEEAKRKKQLGQVEIGSSKPDM
                     EKSHFTRDPSQLKGVLVRASLKKSTMGFGFTIIGGDRPDEFLQVKNVLKDGPAAQDGK
                     IAPGDVIVDINGNCVLGHTHADVVQMFQLVPVNQYVNLTLCRGYPLPDDSEDPVVDIV
                     AATPVINGQSLTKGETCMNPQDFKPGAMVLEQNGKSGHTLTGDGLNGPSDASEQRVSM
                     ASSGSSQPELVTIPLIKGPKGFGFAIADSPTGQKVKMILDSQWCQGLQKGDIIKEIYH
                     QNVQNLTHLQVVEVLKQFPVGADVPLLILRGGPPSPTKTAKMKTDKKENAGSLEAINE
                     PIPQPMPFPPSIIRSGSPKLDPSEVYLKSKTLYEDKPPNTKDLDVFLRKQESGFGFRV
                     LGGDGPDQSIYIGAIIPLGAAEKDGRLRAADELMCIDGIPVKGKSHKQVLDLMTTAAR
                     NGHVLLTVRRKIFYGEKQPEDDSSQAFISTQNGSPRLNRAEVPARPAPQEPYDVVLQR
                     KENEGFGFVILTSKNKPPPGVIPHKIGRVIEGSPADRCGKLKVGDHISAVNGQSIVEL
                     SHDNIVQLIKDAGVTVTLTVIAEEEHHGPPSGTNSARQSPALQHRPMGQSQANHIPGD
                     RSALEGEIGKDVSTSYRHSWSDHKHLAQPDTAVISVVGSRHNQNLGCYPVELERGPRG
                     FGFSLRGGKEYNMGLFILRLAEDGPAIKDGRIHVGDQIVEINGEPTQGITHTRAIELI
                     QAGGNKVLLLLRPGTGLIPDHGDWDINNPSSSNVIYDEQSPLPPSSHFASIFEESHVP
                     VIEESLRVQICEKAEELKDIVPEKKSTLNENQPEIKHQSLLQKNVSKRDPPSSHGHSN
                     KKNLLKVENGVTRRGRSVSPKKPASQHSEEHLDKIPSPLKNNPKRRPRDQSLSPSKGE
                     NKSCQVSTRAGSGQDQCRKSRGRSASPKKQQKIEGSKAPSNAEAKLLEGKSRRIAGYT
                     GSNAEQIPDGKEKSDVIRKDAKQNQLEKSRTRSPEKKIKRMVEKSLPSKMTNKTTSKE
                     VSENEKGKKVTTGETSSSNDKIGENVQLSEKRLKQEPEEKVVSNKTEDHKGKELEAAD
                     KNKETGRFKPESSSPVKKTLITPGPWKVPSGNKVTGTIGMAEKRQ"
     exon            836..952
                     /gene="MAGI3"
                     /gene_synonym="dJ730K3.2; MAGI-3"
                     /inference="alignment:Splign:2.1.0"
     exon            953..1072
                     /gene="MAGI3"
                     /gene_synonym="dJ730K3.2; MAGI-3"
                     /inference="alignment:Splign:2.1.0"
     exon            1073..1282
                     /gene="MAGI3"
                     /gene_synonym="dJ730K3.2; MAGI-3"
                     /inference="alignment:Splign:2.1.0"
     exon            1283..1457
                     /gene="MAGI3"
                     /gene_synonym="dJ730K3.2; MAGI-3"
                     /inference="alignment:Splign:2.1.0"
     exon            1458..1537
                     /gene="MAGI3"
                     /gene_synonym="dJ730K3.2; MAGI-3"
                     /inference="alignment:Splign:2.1.0"
     exon            1538..1595
                     /gene="MAGI3"
                     /gene_synonym="dJ730K3.2; MAGI-3"
                     /inference="alignment:Splign:2.1.0"
     exon            1596..1690
                     /gene="MAGI3"
                     /gene_synonym="dJ730K3.2; MAGI-3"
                     /inference="alignment:Splign:2.1.0"
     exon            1691..1879
                     /gene="MAGI3"
                     /gene_synonym="dJ730K3.2; MAGI-3"
                     /inference="alignment:Splign:2.1.0"
     exon            1880..2485
                     /gene="MAGI3"
                     /gene_synonym="dJ730K3.2; MAGI-3"
                     /inference="alignment:Splign:2.1.0"
     exon            2486..2517
                     /gene="MAGI3"
                     /gene_synonym="dJ730K3.2; MAGI-3"
                     /inference="alignment:Splign:2.1.0"
     exon            2518..2674
                     /gene="MAGI3"
                     /gene_synonym="dJ730K3.2; MAGI-3"
                     /inference="alignment:Splign:2.1.0"
     exon            2675..2766
                     /gene="MAGI3"
                     /gene_synonym="dJ730K3.2; MAGI-3"
                     /inference="alignment:Splign:2.1.0"
     exon            2767..2959
                     /gene="MAGI3"
                     /gene_synonym="dJ730K3.2; MAGI-3"
                     /inference="alignment:Splign:2.1.0"
     exon            2960..3148
                     /gene="MAGI3"
                     /gene_synonym="dJ730K3.2; MAGI-3"
                     /inference="alignment:Splign:2.1.0"
     exon            3149..3334
                     /gene="MAGI3"
                     /gene_synonym="dJ730K3.2; MAGI-3"
                     /inference="alignment:Splign:2.1.0"
     exon            3335..3437
                     /gene="MAGI3"
                     /gene_synonym="dJ730K3.2; MAGI-3"
                     /inference="alignment:Splign:2.1.0"
     exon            3438..3564
                     /gene="MAGI3"
                     /gene_synonym="dJ730K3.2; MAGI-3"
                     /inference="alignment:Splign:2.1.0"
     exon            3565..3708
                     /gene="MAGI3"
                     /gene_synonym="dJ730K3.2; MAGI-3"
                     /inference="alignment:Splign:2.1.0"
     exon            3709..3847
                     /gene="MAGI3"
                     /gene_synonym="dJ730K3.2; MAGI-3"
                     /inference="alignment:Splign:2.1.0"
     exon            3848..6874
                     /gene="MAGI3"
                     /gene_synonym="dJ730K3.2; MAGI-3"
                     /inference="alignment:Splign:2.1.0"
     polyA_site      6874
                     /gene="MAGI3"
                     /gene_synonym="dJ730K3.2; MAGI-3"
                     /note="major polyA site"
ORIGIN      
        1 agtttcctgg cagcttgggc aggcgttggt ctccgcgcta cagctccgca gcggccacga
       61 tcgatcccgc gacgggtcta cgcgcgcttc tgcgcgcccc ccacggattt ttcccgttcc
      121 cggctcttcc cccttcacct ccccgccccc ccgcctcagc ctttcccgcc gctcgggcgc
      181 tgagcccagg gaccagccgg cggcagtgac cccgcccgaa gccccttctg gaacctcctg
      241 gggacaattc ccttttcctt tcttcagcct aacccgcggt ggccctcgcg gagtccggtc
      301 agccccgggg agcgcagccg ggaggggcgt ccaaggggcg tctcgcgtct gggaacggcc
      361 gggcccccag cgggctgtgg tcgcggggtg ggggccggag cggcgaggcc ccccttaccg
      421 ggctgcgcgg gccgcccagg gcccccgggc tgagacgggg ccggagcggc gccccggccg
      481 cccgcgcggg gtctccccca tggtgcagcg gggttcggga tgtcgaagac gctgaagaag
      541 aagaagcact ggctcagcaa ggtgcaggag tgcgccgtgt cctgggccgg gcccccgggc
      601 gacttcggcg cggagatccg cggtggcgcg gagcgtggcg agttccccta cctggggcgg
      661 ctccgcgagg agcccggcgg gggcacctgc tgcgtcgtct cgggcaaggc gcccagccca
      721 ggcgatgtgc tgctggaggt aaacgggacg cctgtcagcg ggctcaccaa ccgggacacc
      781 ctggctgtca tccgccactt ccgcgagccc atccgtctca agactgtgaa accaggcaaa
      841 gtcattaata aagatttgcg gcattaccta agtcttcagt ttcaaaaagg atcaattgac
      901 cacaaactgc agcaagtgat cagagataat ctctacttga gaaccattcc atgcactaca
      961 agggccccca gggatggaga agtaccagga gtggattata atttcatttc cgttgaacag
     1021 ttcaaagcac tggaagagag tggagcattg ttagaaagtg ggacatatga tggaaacttc
     1081 tatggaactc ccaagcctcc agcagaaccc agcccttttc agccagatcc agttgatcaa
     1141 gtcctctttg ataatgagtt tgatgcagaa tctcaaagaa aacgaacgac atctgtcagc
     1201 aagatggaaa gaatggatag ctctcttcct gaagaggaag aagatgagga caaggaagct
     1261 attaatggca gtggaaacgc agaaaacaga gagaggcatt ctgagtcatc tgactggatg
     1321 aagactgttc caagttacaa ccaaacaaat agctccatgg actttagaaa ttatatgatg
     1381 agagatgaga ctctggaacc actgcccaaa aactgggaaa tggcctacac tgacacaggg
     1441 atgatctact tcattgacca caataccaag acaaccacct ggttggatcc tcgtctttgt
     1501 aagaaagcca aagcccctga agactgtgaa gatggagagc ttccttatgg ctgggagaaa
     1561 atagaggacc ctcagtatgg gacatactat gttgatcacc ttaaccagaa aacccagttt
     1621 gaaaatccag tggaggaagc caaaaggaaa aagcagttag gacaggttga aattgggtct
     1681 tcaaaaccag atatggaaaa atcacacttc acaagagatc catcccagct taaaggtgtc
     1741 cttgttcgag catcactgaa aaaaagcaca atgggatttg gttttactat tattggtgga
     1801 gatagacctg atgagttcct acaagtgaaa aatgtgctga aagatggtcc cgcagctcag
     1861 gatgggaaaa ttgcaccagg cgatgttatt gtagacatca atggcaactg tgtcctcggt
     1921 cacactcatg cagatgttgt ccagatgttt caattggtac ctgtcaatca gtatgtaaac
     1981 ctcactttat gtcgtggtta tccacttcct gatgacagtg aagatcctgt tgtggacatt
     2041 gttgctgcta cccctgtcat caatggacag tcattaacca agggagagac ttgcatgaat
     2101 cctcaggatt ttaagccagg agcaatggtt ctggagcaga atggaaaatc gggacacact
     2161 ttgactggtg atggtctcaa tggaccatca gatgcaagtg agcagagagt atccatggca
     2221 tcgtcaggca gctcccagcc tgaactagtg actatccctt tgattaaggg ccctaaaggg
     2281 tttgggtttg caattgctga cagccctact ggacagaagg tgaaaatgat actggatagt
     2341 cagtggtgtc aaggccttca gaaaggagat ataattaagg aaatatacca tcaaaatgtg
     2401 cagaatttaa cacatctcca agtggtagag gtgctaaagc agtttccagt aggtgctgat
     2461 gtaccattgc ttatcttaag aggaggtcct ccttcaccaa ccaaaactgc caaaatgaaa
     2521 acagataaaa aggaaaatgc aggaagtttg gaggccataa atgagcctat tcctcagcct
     2581 atgccttttc caccgagcat tatcaggtca ggatccccaa aattggatcc ttctgaggtc
     2641 tacctgaaat ctaagacttt atatgaagat aaaccaccaa acaccaaaga tttggatgtt
     2701 tttcttcgaa aacaagagtc agggtttggc ttcagggtgc taggaggaga tggacctgac
     2761 cagtctatat atattggggc tattattccc ctgggagcag ctgagaaaga tggtcggctc
     2821 cgcgcagctg atgaactaat gtgcattgat ggaattcctg ttaaagggaa atcacacaaa
     2881 caagtcttgg acctcatgac aactgctgct cgaaatggcc atgtgttact aactgtcaga
     2941 cggaagatct tctatggaga aaaacaaccc gaggacgaca gctctcaggc cttcatttca
     3001 acacagaatg gatctccccg cctgaaccgg gcagaggtcc cagccaggcc tgcaccccag
     3061 gagccctatg atgttgtctt gcaacgaaaa gaaaatgaag gatttggctt tgtcatcctc
     3121 acctccaaaa acaaaccacc tccaggagtt attcctcata aaattggccg agtcatagaa
     3181 ggaagtccgg ctgaccgctg tggaaaactg aaagttggag atcatatctc tgcagtgaat
     3241 gggcagtcca ttgttgaact gtctcatgat aacattgttc agctgatcaa agatgctggt
     3301 gtcaccgtca cactaacggt cattgctgaa gaagagcatc atggtccacc atcaggaaca
     3361 aactcagcca ggcaaagccc agccctgcag cacaggccca tgggacagtc acaggccaac
     3421 cacatacctg gggacagaag tgccctagaa ggtgaaattg gaaaagatgt ctccacttct
     3481 tacagacatt cttggtcaga ccacaagcac cttgcacagc ctgacaccgc agtaatttca
     3541 gttgtaggca gtcggcacaa tcagaacctt ggttgttatc cagtagagct ggagagaggc
     3601 ccccggggct ttggattcag cctccgaggg gggaaggagt acaacatggg gctgttcatc
     3661 cttcgtcttg ctgaagatgg tcctgccatc aaagatggca gaattcatgt tggtgaccag
     3721 attgttgaaa tcaatgggga acctacacaa ggaatcacac atactcgagc aattgagctc
     3781 attcaggctg gtggaaataa agttcttctt cttttgaggc caggaactgg cttgatacct
     3841 gaccatggtg attgggatat taataatcct tcgtcttcaa atgtgattta tgatgaacag
     3901 tcaccattac ccccatcttc acattttgct tccatatttg aagagtctca cgtgccagta
     3961 attgaagaat ctttgagagt tcagatatgt gaaaaggcag aagaattaaa ggacattgtg
     4021 cctgaaaaga aaagcacttt aaatgaaaat cagcctgaga taaagcatca gtctcttctc
     4081 cagaaaaatg tgagtaagag ggatccaccc agcagtcatg ggcacagtaa caagaaaaat
     4141 ctattaaaag tagaaaatgg tgttacacga agaggtagat cggttagtcc caaaaagcca
     4201 gccagtcaac attcagagga acatttggat aagattccta gtcctctaaa aaataacccc
     4261 aaaagaagac ccagagatca atccctcagc cccagcaaag gggaaaataa aagttgtcag
     4321 gtcagcacca gggcaggctc tggacaagat cagtgcagaa aaagcagagg tcggtcggcc
     4381 agcccaaaaa agcagcaaaa aattgaagga agcaaagctc catcaaatgc tgaggccaaa
     4441 ttattagagg gtaagagtcg aagaatagca ggctatacgg gcagtaatgc tgagcagatc
     4501 ccagatggga aggaaaaatc agacgtcatc aggaaagatg caaagcagaa tcagttggaa
     4561 aaaagcagaa caaggtctcc agagaaaaaa atcaaaagaa tggttgagaa atctcttcca
     4621 tccaaaatga ctaataagac tacaagtaaa gaagtatctg aaaatgaaaa aggaaagaaa
     4681 gtaaccacag gagaaacaag ttctagtaac gataaaatag gagaaaatgt ccagctatca
     4741 gaaaagaggc tgaagcaaga acctgaagag aaggtagttt caaacaaaac agaagatcac
     4801 aaagggaaag aactagaggc agctgacaaa aacaaagaga ctggaaggtt caaaccggaa
     4861 agcagttctc cagttaagaa aacactgata actccagggc cctggaaggt tccaagtgga
     4921 aataaagtca caggcactat tggtatggct gagaaacggc agtaaccttt agtataaaac
     4981 aaagaaaaac aagttgtaat cttttcttac agcagcattt ttccagaaaa agcctttttt
     5041 tttttttcag atattctgaa acagataagt acatgttaat gtgagcctca agttacctag
     5101 gctgcatgaa gggcctttag gattgctaag aaccaactgt ccccctggcc ggctgccctc
     5161 cctcgctctc aggaaggagc tgcatccaca tgctcatctg acccgccctg ctcaggctgc
     5221 ccagctcgtc ttcatgagtg tctgaacaaa tgacatatgt tgatattaac aatgtggtca
     5281 caactcactt tgtatttgtg ccaagttatc tactgtatca tgtctgtttt tatccttttt
     5341 gttcagctgt ttccacagta atgaaaaagt taggtttggc ttggaagttg atgatctcaa
     5401 tagcatgttg catgtttaca gagagaaata tgtgagtcct tgcagaagaa gagactgtta
     5461 actcatcgtt aaagatggcc gttgtctctt ctaacagcta ctgatgatgt cccactttaa
     5521 aaataaaacc cccaaacatc actactttaa ggaaaaaaaa aatgtagtcc aatattgatg
     5581 ctttcttatg gctttttatt ttaatttggc tggataagtt gtttcaaata actgttaaag
     5641 atattactta caattgaatg tttgaaataa gaaagtactt taagcaatag agttcatctc
     5701 ctgctgtgtt atccaacctc gatgtatact tacagcatct caggtcaccc tttttatttc
     5761 agttatttaa ttatgaaacc ataaagaagc atgtggaaat agtgtttatt gctctttgaa
     5821 gaaaaaccac caactatttc tggatatttt ggctgtacct actactaaag tcattagtct
     5881 ttaatacata atacatattt gaaaagtaaa catattatat agattatgtg agggacttaa
     5941 tcatgaaacc agtttcacag tccaagtacc aactcttctg gtagcaggtg cacaagcttg
     6001 ggtgtttaaa aacaacctgt gtagggtatg cccagcaaat gaggacaaat gtgtagacag
     6061 tacttactgg atcttattta acttttagct acattaacta actttcttat ttaaaaacaa
     6121 gaaagggaga ctaaacatct gcttaacttg tacacatttt cagaattctt tttaaaagtc
     6181 tagttaaaga tgtttcttag aagttggaga ctgttaacaa cttccataaa atagatccag
     6241 gtttttcagt tccctgaagc agcattcagt agcatctata taaataaagg caccttctga
     6301 gaataaaact attttatgga gtgtgtgaac acacttgttc tgtcacctgg gttcatcttg
     6361 ttgtgaagca cattaggtcc aggtccttcc ctctgggagt ctgactgtga aactctttaa
     6421 cccaacaact caattagccc ctgtagataa gacatgcttc ccagagtgag atttttgaaa
     6481 tccccttttc atccagaact atatttaccc acctattgta actattcaaa tagagcaaaa
     6541 ttaggaggct tgataaatac taagaattta gtaccacaga aattatttat tattttccct
     6601 gtagtccaca attagtgata acgaatccta tttttgttaa ctgtgacata actttgatgt
     6661 catatgttgt cctatgtggt tcttcctaag taaactctgt actgattata tactgactta
     6721 gcaatgtggc cttggaatgc tgagcaaaat gtggatgtac tggttgtaaa tgtttatata
     6781 ttgtacagta cctttatata tacacttgag gttctgatta gagaaagatc tgtaaattgc
     6841 tcattatttt ttatatagat atttaaaaaa aaca
//
