LOCUS       NM_032272               1715 bp    mRNA    linear   PRI 19-SEP-2024
DEFINITION  Homo sapiens MAF1 homolog, negative regulator of RNA polymerase III
            (MAF1), mRNA.
ACCESSION   NM_032272
VERSION     NM_032272.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1715)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   2  (bases 1 to 1715)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   3  (bases 1 to 1715)
  AUTHORS   Lai,C., Zhang,J., Tan,Z., Shen,L.F., Zhou,R.R. and Zhang,Y.Y.
  TITLE     Maf1 suppression of ATF5-dependent mitochondrial unfolded protein
            response contributes to rapamycin-induced radio-sensitivity in lung
            cancer cell line A549
  JOURNAL   Aging (Albany NY) 13 (5), 7300-7313 (2021)
   PUBMED   33640883
  REMARK    GeneRIF: Maf1 suppression of ATF5-dependent mitochondrial unfolded
            protein response contributes to rapamycin-induced radio-sensitivity
            in lung cancer cell line A549.
REFERENCE   4  (bases 1 to 1715)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   5  (bases 1 to 1715)
  AUTHORS   Vorlander,M.K., Baudin,F., Moir,R.D., Wetzel,R., Hagen,W.J.H.,
            Willis,I.M. and Muller,C.W.
  TITLE     Structural basis for RNA polymerase III transcription repression by
            Maf1
  JOURNAL   Nat Struct Mol Biol 27 (3), 229-232 (2020)
   PUBMED   32066962
  REMARK    GeneRIF: Structural basis for RNA polymerase III transcription
            repression by Maf1 in humans and yeast has been uncovered.
REFERENCE   6  (bases 1 to 1715)
  AUTHORS   Johnson,S.S., Zhang,C., Fromm,J., Willis,I.M. and Johnson,D.L.
  TITLE     Mammalian Maf1 is a negative regulator of transcription by all
            three nuclear RNA polymerases
  JOURNAL   Mol Cell 26 (3), 367-379 (2007)
   PUBMED   17499043
  REMARK    GeneRIF: The human Maf1 protein negatively regulates transcription
            by all three nuclear Pols. Changes in Maf1 expression affect Pol I-
            and Pol III-dependent transcription in human glioblastoma lines.
REFERENCE   7  (bases 1 to 1715)
  AUTHORS   Reina,J.H., Azzouz,T.N. and Hernandez,N.
  TITLE     Maf1, a new player in the regulation of human RNA polymerase III
            transcription
  JOURNAL   PLoS One 1 (1), e134 (2006)
   PUBMED   17205138
  REMARK    GeneRIF: Maf1 can be co-immunoprecipitated with pol III and
            associates in vitro with two pol III subunits, the largest subunit
            RPC1 and the alpha-like subunit RPAC2. Maf1 represses pol III
            transcription in vitro and in vivo.
            Publication Status: Online-Only
REFERENCE   8  (bases 1 to 1715)
  AUTHORS   Upadhya,R., Lee,J. and Willis,I.M.
  TITLE     Maf1 is an essential mediator of diverse signals that repress RNA
            polymerase III transcription
  JOURNAL   Mol Cell 10 (6), 1489-1494 (2002)
   PUBMED   12504022
  REMARK    GeneRIF: Functional studies of the yeast counterpart.
REFERENCE   9  (bases 1 to 1715)
  AUTHORS   Pluta,K., Lefebvre,O., Martin,N.C., Smagowicz,W.J., Stanford,D.R.,
            Ellis,S.R., Hopper,A.K., Sentenac,A. and Boguta,M.
  TITLE     Maf1p, a negative effector of RNA polymerase III in Saccharomyces
            cerevisiae
  JOURNAL   Mol Cell Biol 21 (15), 5031-5040 (2001)
   PUBMED   11438659
  REMARK    GeneRIF: Functional studies of the yeast counterpart.
REFERENCE   10 (bases 1 to 1715)
  AUTHORS   Simpson,J.C., Wellenreuther,R., Poustka,A., Pepperkok,R. and
            Wiemann,S.
  TITLE     Systematic subcellular localization of novel proteins identified by
            large-scale cDNA sequencing
  JOURNAL   EMBO Rep 1 (3), 287-292 (2000)
   PUBMED   11256614
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DN997000.1, BM721318.1,
            BE093055.1, BC031273.1, BF339064.1 and BC018714.1.
            
            On Nov 23, 2018 this sequence version replaced NM_032272.4.
            
            Summary: This gene encodes a protein that is similar to Maf1, a
            Saccharomyces cerevisiae protein highly conserved in eukaryotic
            cells. Yeast Maf1 is a negative effector of RNA polymerase III (Pol
            III). It responds to changes in the cellular environment and
            represses pol III transcription. Biochemical studies identified the
            initiation factor TFIIIB as a target for Maf1-dependent repression.
            [provided by RefSeq, Jul 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR3476690.76158.1,
                                           SRR3476690.406554.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000322428.10/ ENSP00000318604.5
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-347               DN997000.1         69-415
            348-501             BM721318.1         313-466
            502-820             BE093055.1         22-340
            821-1147            BC031273.1         782-1108
            1148-1572           BF339064.1         120-544
            1573-1715           BC018714.1         1531-1673
FEATURES             Location/Qualifiers
     source          1..1715
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="8"
                     /map="8q24.3"
     gene            1..1715
                     /gene="MAF1"
                     /note="MAF1 homolog, negative regulator of RNA polymerase
                     III"
                     /db_xref="GeneID:84232"
                     /db_xref="HGNC:HGNC:24966"
                     /db_xref="MIM:610210"
     exon            1..398
                     /gene="MAF1"
                     /inference="alignment:Splign:2.1.0"
     exon            399..525
                     /gene="MAF1"
                     /inference="alignment:Splign:2.1.0"
     CDS             443..1213
                     /gene="MAF1"
                     /note="homolog of yeast MAF1; MAF1 negative regulator of
                     RNA polymerase III"
                     /codon_start=1
                     /product="repressor of RNA polymerase III transcription
                     MAF1 homolog"
                     /protein_id="NP_115648.2"
                     /db_xref="CCDS:CCDS6416.1"
                     /db_xref="GeneID:84232"
                     /db_xref="HGNC:HGNC:24966"
                     /db_xref="MIM:610210"
                     /translation="MKLLENSSFEAINSQLTVETGDAHIIGRIESYSCKMAGDDKHMF
                     KQFCQEGQPHVLEALSPPQTSGLSPSRLSKSQGGEEEGPLSDKCSRKTLFYLIATLNE
                     SFRPDYDFSTARSHEFSREPSLSWVVNAVNCSLFSAVREDFKDLKPQLWNAVDEEICL
                     AECDIYSYNPDLDSDPFGEDGSLWSFNYFFYNKRLKRIVFFSCRSISGSTYTPSEAGN
                     ELDMELGEEEVEEESRSGGSGAEETSTMEEDRVPVICI"
     exon            526..654
                     /gene="MAF1"
                     /inference="alignment:Splign:2.1.0"
     exon            655..820
                     /gene="MAF1"
                     /inference="alignment:Splign:2.1.0"
     exon            821..942
                     /gene="MAF1"
                     /inference="alignment:Splign:2.1.0"
     exon            943..1062
                     /gene="MAF1"
                     /inference="alignment:Splign:2.1.0"
     exon            1063..1191
                     /gene="MAF1"
                     /inference="alignment:Splign:2.1.0"
     exon            1192..1715
                     /gene="MAF1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1695..1700
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MAF1"
                     /note="hexamer: AATAAA"
     polyA_site      1715
                     /gene="MAF1"
                     /note="major polyA site"
ORIGIN      
        1 attcagaggc gcggggccgg gcccggcgga cgcttgttgt tgtccggccg ggggaggcgg
       61 aggtcgctcg ctcgctcgct cggctcgctg actcgccgga gcgctctgtg gcggtcggcg
      121 gcaggtcggt cgcgagagcg ggctctgtgg aagggggcga ggctatgtcg cggtggcagc
      181 ccggatgggc cggcagggcc gggagtaacg ggacgtcgcc gcggagcttc ttcccccgga
      241 tacagtgcgg cccgagcgga ggccgcggcg ccgccctccg atcttgaaga gcccgcgctg
      301 cgcggagccc gcccccgcct gcgcaccggc accgacgcgg agcgacccag cccagccaga
      361 cccggcccgg cgcggcctga tctaacccag ccaggcaggc aatactagcc cctctggagc
      421 acggagctcc ttccccaaag acatgaagct attggagaac tcgagctttg aagccatcaa
      481 ctcacagctg actgtggaga ctggagatgc ccacatcatt ggcaggattg agagctactc
      541 atgtaagatg gcaggagacg acaaacacat gttcaagcag ttctgccagg agggccagcc
      601 ccacgtgctg gaggcacttt ctccacccca gacttcagga ctgagcccca gcagactcag
      661 caaaagccaa ggcggtgagg aggagggccc cctcagtgac aagtgcagcc gcaagaccct
      721 cttctacctg attgccacgc tcaatgagtc cttcaggcct gactatgact tcagcacagc
      781 ccgcagccat gagttcagcc gggagcccag ccttagctgg gtggtgaatg cagtcaactg
      841 cagtctgttc tcagctgtgc gggaggactt caaggatctg aaaccacagc tgtggaacgc
      901 ggtggacgag gagatctgcc tggctgaatg tgacatctac agctataacc cagacttgga
      961 ctcagatccc ttcggggagg atggtagcct ctggtccttc aactacttct tctacaacaa
     1021 gcggctcaag cgaatcgtct tctttagctg ccgttccatc agtggctcca cctacacacc
     1081 ctcagaggca ggcaacgagc tggacatgga gctgggggag gaggaggtgg aggaagaaag
     1141 cagaagcggg ggcagtgggg ccgaggagac cagcaccatg gaggaggaca gggtcccagt
     1201 gatctgtatt tgatgaggag gagccgaggc cccagcttca tccagcttca accaatgcct
     1261 ggacctgtcc acctgagagg cccctggggc ctccccagct gctggccaga ccctggcgct
     1321 gccacagtcc tggcactgcc caaggccata cctgcctagc cctttggctc catcctgtgg
     1381 atgcccactc acccctcaga ctcctgctgc ccatgctgtg gccggacttg tcagcagggg
     1441 gcctggtggg aggagcgact gccctgccca aatgaactgc cacagcaggg acagctggac
     1501 cgcagagttt atttttgtat ttctactggg cctgcacact ccagcccaaa gggtctgtgg
     1561 ccggaggccc cacgagcagg ccccagcagt caccggctct ggtcttgggc cggccccggt
     1621 gcccacctgt acccccacct cgcccatttg gccgcgtgca ctgagtgtca ctttgctgca
     1681 gctcgtttct ttccaataaa agtttctgtg actta
//
