LOCUS       NM_000081              13466 bp    mRNA    linear   PRI 12-OCT-2024
DEFINITION  Homo sapiens lysosomal trafficking regulator (LYST), transcript
            variant 1, mRNA.
ACCESSION   NM_000081
VERSION     NM_000081.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 13466)
  AUTHORS   Morimoto,M., Nicoli,E.R., Kuptanon,C., Roney,J.C.,
            Serra-Vinardell,J., Sharma,P., Adams,D.R., Gallin,J.I.,
            Holland,S.M., Rosenzweig,S.D., Barbot,J., Ciccone,C., Huizing,M.,
            Toro,C., Gahl,W.A., Introne,W.J. and Malicdan,M.C.V.
  TITLE     Spectrum of LYST mutations in Chediak-Higashi syndrome: a report of
            novel variants and a comprehensive review of the literature
  JOURNAL   J Med Genet 61 (3), 212-223 (2024)
   PUBMED   37788905
  REMARK    GeneRIF: Spectrum of LYST mutations in Chediak-Higashi syndrome: a
            report of novel variants and a comprehensive review of the
            literature.
            Review article
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13466)
  AUTHORS   Wan,W., Zhang,S., Zhao,M., OuYang,X., Yu,Y., Xiong,X., Zhao,N. and
            Jiao,J.
  TITLE     Lysosomal trafficking regulator restricts intracellular growth of
            Coxiella burnetii by inhibiting the expansion of
            Coxiella-containing vacuole and upregulating nos2 expression
  JOURNAL   Front Cell Infect Microbiol 13, 1336600 (2024)
   PUBMED   38282619
  REMARK    GeneRIF: Lysosomal trafficking regulator restricts intracellular
            growth of Coxiella burnetii by inhibiting the expansion of
            Coxiella-containing vacuole and upregulating nos2 expression.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 13466)
  AUTHORS   Serra-Vinardell,J., Sandler,M.B., De Pace,R., Manzella-Lapeira,J.,
            Cougnoux,A., Keyvanfar,K., Introne,W.J., Brzostowski,J.A.,
            Ward,M.E., Gahl,W.A., Sharma,P. and Malicdan,M.C.V.
  TITLE     LYST deficiency impairs autophagic lysosome reformation in neurons
            and alters lysosome number and size
  JOURNAL   Cell Mol Life Sci 80 (2), 53 (2023)
   PUBMED   36707427
  REMARK    GeneRIF: LYST deficiency impairs autophagic lysosome reformation in
            neurons and alters lysosome number and size.
            Erratum:[Cell Mol Life Sci. 2023 Mar 4;80(3):81. doi:
            10.1007/s00018-023-04724-9. PMID: 36869920]
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 13466)
  AUTHORS   Karim,M.A., Suzuki,K., Fukai,K., Oh,J., Nagle,D.L., Moore,K.J.,
            Barbosa,E., Falik-Borenstein,T., Filipovich,A., Ishida,Y.,
            Kivrikko,S., Klein,C., Kreuz,F., Levin,A., Miyajima,H.,
            Regueiro,J.R., Russo,C., Uyama,E., Vierimaa,O. and Spritz,R.A.
  TITLE     Apparent genotype-phenotype correlation in childhood, adolescent,
            and adult Chediak-Higashi syndrome
  JOURNAL   Am J Med Genet 108 (1), 16-22 (2002)
   PUBMED   11857544
  REMARK    GeneRIF: Childhood, adolescent, and adult forms of Chediak-Higashi
            syndrome correlate with different genotypes.
REFERENCE   5  (bases 1 to 13466)
  AUTHORS   Barbosa,M.D., Barrat,F.J., Tchernev,V.T., Nguyen,Q.A., Mishra,V.S.,
            Colman,S.D., Pastural,E., Dufourcq-Lagelouse,R., Fischer,A.,
            Holcombe,R.F., Wallace,M.R., Brandt,S.J., de Saint Basile,G. and
            Kingsmore,S.F.
  TITLE     Identification of mutations in two major mRNA isoforms of the
            Chediak-Higashi syndrome gene in human and mouse
  JOURNAL   Hum Mol Genet 6 (7), 1091-1098 (1997)
   PUBMED   9215680
REFERENCE   6  (bases 1 to 13466)
  AUTHORS   Nagle,D.L., Karim,M.A., Woolf,E.A., Holmgren,L., Bork,P.,
            Misumi,D.J., McGrail,S.H., Dussault,B.J. Jr., Perou,C.M.,
            Boissy,R.E., Duyk,G.M., Spritz,R.A. and Moore,K.J.
  TITLE     Identification and mutation analysis of the complete gene for
            Chediak-Higashi syndrome
  JOURNAL   Nat Genet 14 (3), 307-311 (1996)
   PUBMED   8896560
REFERENCE   7  (bases 1 to 13466)
  AUTHORS   Barbosa,M.D., Nguyen,Q.A., Tchernev,V.T., Ashley,J.A., Detter,J.C.,
            Blaydes,S.M., Brandt,S.J., Chotai,D., Hodgman,C., Solari,R.C.,
            Lovett,M. and Kingsmore,S.F.
  TITLE     Identification of the homologous beige and Chediak-Higashi syndrome
            genes
  JOURNAL   Nature 382 (6588), 262-265 (1996)
   PUBMED   8717042
  REMARK    Erratum:[Nature 1997 Jan 2;385(6611):97]
REFERENCE   8  (bases 1 to 13466)
  AUTHORS   Toro,C., Morimoto,M., Malicdan,M.C., Adams,D.R. and Introne,W.J.
  TITLE     Chediak-Higashi Syndrome
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301751
REFERENCE   9  (bases 1 to 13466)
  AUTHORS   Jenkins,N.A., Justice,M.J., Gilbert,D.J., Chu,M.L. and
            Copeland,N.G.
  TITLE     Nidogen/entactin (Nid) maps to the proximal end of mouse chromosome
            13 linked to beige (bg) and identifies a new region of homology
            between mouse and human chromosomes
  JOURNAL   Genomics 9 (2), 401-403 (1991)
   PUBMED   1672300
REFERENCE   10 (bases 1 to 13466)
  AUTHORS   Merino,F., Amesty,C., Henle,W., Layrisse,Z., Bianco,N. and
            Ramirez-Duque,P.
  TITLE     Chediak-Higashi syndrome: immunological responses to Epstein-Barr
            virus studies in gene heterozygotes
  JOURNAL   J Clin Immunol 6 (3), 242-248 (1986)
   PUBMED   3013924
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from U67615.1, BI031463.1,
            BX649113.1 and AA486745.1.
            
            On Aug 31, 2019 this sequence version replaced NM_000081.3.
            
            Summary: This gene encodes a protein that regulates intracellular
            protein trafficking in endosomes, and may be involved in
            pigmentation. Mutations in this gene are associated with
            Chediak-Higashi syndrome, a lysosomal storage disorder. Alternative
            splicing results in multiple transcript variants, though the
            full-length nature of some of these variants has not been
            determined. [provided by RefSeq, Apr 2013].
            
            Transcript Variant: This variant (1) encodes the functional
            protein. Variants 1 and 3 encode the same protein.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##RefSeq-Attributes-START##
            inferred exon combination :: based on alignments, homology
            MANE Ensembl match        :: ENST00000389793.7/ ENSP00000374443.2
            RefSeq Select criteria    :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-3634              U67615.1           29-3662
            3635-3642           BI031463.1         343-350
            3643-10696          U67615.1           3671-10724
            10697-10705         BX649113.1         5528-5536
            10706-11933         U67615.1           10734-11961
            11934-13464         BX649113.1         6765-8295
            13465-13466         AA486745.1         17-18               c
FEATURES             Location/Qualifiers
     source          1..13466
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1q42.3"
     gene            1..13466
                     /gene="LYST"
                     /gene_synonym="CHS; CHS1; Mauve"
                     /note="lysosomal trafficking regulator"
                     /db_xref="GeneID:1130"
                     /db_xref="HGNC:HGNC:1968"
                     /db_xref="MIM:606897"
     exon            1..64
                     /gene="LYST"
                     /gene_synonym="CHS; CHS1; Mauve"
                     /inference="alignment:Splign:2.1.0"
     exon            65..154
                     /gene="LYST"
                     /gene_synonym="CHS; CHS1; Mauve"
                     /inference="alignment:Splign:2.1.0"
     exon            155..353
                     /gene="LYST"
                     /gene_synonym="CHS; CHS1; Mauve"
                     /inference="alignment:Splign:2.1.0"
     exon            354..444
                     /gene="LYST"
                     /gene_synonym="CHS; CHS1; Mauve"
                     /inference="alignment:Splign:2.1.0"
     exon            445..2524
                     /gene="LYST"
                     /gene_synonym="CHS; CHS1; Mauve"
                     /inference="alignment:Splign:2.1.0"
     exon            2525..3554
                     /gene="LYST"
                     /gene_synonym="CHS; CHS1; Mauve"
                     /inference="alignment:Splign:2.1.0"
     exon            3555..3716
                     /gene="LYST"
                     /gene_synonym="CHS; CHS1; Mauve"
                     /inference="alignment:Splign:2.1.0"
     exon            3717..3873
                     /gene="LYST"
                     /gene_synonym="CHS; CHS1; Mauve"
                     /inference="alignment:Splign:2.1.0"
     exon            3874..4100
                     /gene="LYST"
                     /gene_synonym="CHS; CHS1; Mauve"
                     /inference="alignment:Splign:2.1.0"
     exon            4101..4167
                     /gene="LYST"
                     /gene_synonym="CHS; CHS1; Mauve"
                     /inference="alignment:Splign:2.1.0"
     exon            4168..4277
                     /gene="LYST"
                     /gene_synonym="CHS; CHS1; Mauve"
                     /inference="alignment:Splign:2.1.0"
     exon            4278..4704
                     /gene="LYST"
                     /gene_synonym="CHS; CHS1; Mauve"
                     /inference="alignment:Splign:2.1.0"
     exon            4705..4849
                     /gene="LYST"
                     /gene_synonym="CHS; CHS1; Mauve"
                     /inference="alignment:Splign:2.1.0"
     exon            4850..5023
                     /gene="LYST"
                     /gene_synonym="CHS; CHS1; Mauve"
                     /inference="alignment:Splign:2.1.0"
     exon            5024..5184
                     /gene="LYST"
                     /gene_synonym="CHS; CHS1; Mauve"
                     /inference="alignment:Splign:2.1.0"
     exon            5185..5375
                     /gene="LYST"
                     /gene_synonym="CHS; CHS1; Mauve"
                     /inference="alignment:Splign:2.1.0"
     exon            5376..5621
                     /gene="LYST"
                     /gene_synonym="CHS; CHS1; Mauve"
                     /inference="alignment:Splign:2.1.0"
     exon            5622..5795
                     /gene="LYST"
                     /gene_synonym="CHS; CHS1; Mauve"
                     /inference="alignment:Splign:2.1.0"
     exon            5796..5945
                     /gene="LYST"
                     /gene_synonym="CHS; CHS1; Mauve"
                     /inference="alignment:Splign:2.1.0"
     exon            5946..6083
                     /gene="LYST"
                     /gene_synonym="CHS; CHS1; Mauve"
                     /inference="alignment:Splign:2.1.0"
     exon            6084..6282
                     /gene="LYST"
                     /gene_synonym="CHS; CHS1; Mauve"
                     /inference="alignment:Splign:2.1.0"
     exon            6283..6414
                     /gene="LYST"
                     /gene_synonym="CHS; CHS1; Mauve"
                     /inference="alignment:Splign:2.1.0"
     exon            6415..7042
                     /gene="LYST"
                     /gene_synonym="CHS; CHS1; Mauve"
                     /inference="alignment:Splign:2.1.0"
     exon            7043..7220
                     /gene="LYST"
                     /gene_synonym="CHS; CHS1; Mauve"
                     /inference="alignment:Splign:2.1.0"
     exon            7221..7390
                     /gene="LYST"
                     /gene_synonym="CHS; CHS1; Mauve"
                     /inference="alignment:Splign:2.1.0"
     exon            7391..7621
                     /gene="LYST"
                     /gene_synonym="CHS; CHS1; Mauve"
                     /inference="alignment:Splign:2.1.0"
     exon            7622..7788
                     /gene="LYST"
                     /gene_synonym="CHS; CHS1; Mauve"
                     /inference="alignment:Splign:2.1.0"
     exon            7789..7941
                     /gene="LYST"
                     /gene_synonym="CHS; CHS1; Mauve"
                     /inference="alignment:Splign:2.1.0"
     exon            7942..8133
                     /gene="LYST"
                     /gene_synonym="CHS; CHS1; Mauve"
                     /inference="alignment:Splign:2.1.0"
     exon            8134..8312
                     /gene="LYST"
                     /gene_synonym="CHS; CHS1; Mauve"
                     /inference="alignment:Splign:2.1.0"
     exon            8313..8519
                     /gene="LYST"
                     /gene_synonym="CHS; CHS1; Mauve"
                     /inference="alignment:Splign:2.1.0"
     exon            8520..8696
                     /gene="LYST"
                     /gene_synonym="CHS; CHS1; Mauve"
                     /inference="alignment:Splign:2.1.0"
     exon            8697..8773
                     /gene="LYST"
                     /gene_synonym="CHS; CHS1; Mauve"
                     /inference="alignment:Splign:2.1.0"
     exon            8774..8962
                     /gene="LYST"
                     /gene_synonym="CHS; CHS1; Mauve"
                     /inference="alignment:Splign:2.1.0"
     exon            8963..9108
                     /gene="LYST"
                     /gene_synonym="CHS; CHS1; Mauve"
                     /inference="alignment:Splign:2.1.0"
     exon            9109..9205
                     /gene="LYST"
                     /gene_synonym="CHS; CHS1; Mauve"
                     /inference="alignment:Splign:2.1.0"
     exon            9206..9267
                     /gene="LYST"
                     /gene_synonym="CHS; CHS1; Mauve"
                     /inference="alignment:Splign:2.1.0"
     exon            9268..9323
                     /gene="LYST"
                     /gene_synonym="CHS; CHS1; Mauve"
                     /inference="alignment:Splign:2.1.0"
     exon            9324..9476
                     /gene="LYST"
                     /gene_synonym="CHS; CHS1; Mauve"
                     /inference="alignment:Splign:2.1.0"
     exon            9477..9721
                     /gene="LYST"
                     /gene_synonym="CHS; CHS1; Mauve"
                     /inference="alignment:Splign:2.1.0"
     exon            9722..9788
                     /gene="LYST"
                     /gene_synonym="CHS; CHS1; Mauve"
                     /inference="alignment:Splign:2.1.0"
     exon            9789..9945
                     /gene="LYST"
                     /gene_synonym="CHS; CHS1; Mauve"
                     /inference="alignment:Splign:2.1.0"
     exon            9946..10086
                     /gene="LYST"
                     /gene_synonym="CHS; CHS1; Mauve"
                     /inference="alignment:Splign:2.1.0"
     exon            10087..10304
                     /gene="LYST"
                     /gene_synonym="CHS; CHS1; Mauve"
                     /inference="alignment:Splign:2.1.0"
     exon            10305..10535
                     /gene="LYST"
                     /gene_synonym="CHS; CHS1; Mauve"
                     /inference="alignment:Splign:2.1.0"
     exon            10536..10725
                     /gene="LYST"
                     /gene_synonym="CHS; CHS1; Mauve"
                     /inference="alignment:Splign:2.1.0"
     exon            10726..10862
                     /gene="LYST"
                     /gene_synonym="CHS; CHS1; Mauve"
                     /inference="alignment:Splign:2.1.0"
     exon            10863..10961
                     /gene="LYST"
                     /gene_synonym="CHS; CHS1; Mauve"
                     /inference="alignment:Splign:2.1.0"
     exon            10962..11101
                     /gene="LYST"
                     /gene_synonym="CHS; CHS1; Mauve"
                     /inference="alignment:Splign:2.1.0"
     exon            11102..11199
                     /gene="LYST"
                     /gene_synonym="CHS; CHS1; Mauve"
                     /inference="alignment:Splign:2.1.0"
     exon            11200..11356
                     /gene="LYST"
                     /gene_synonym="CHS; CHS1; Mauve"
                     /inference="alignment:Splign:2.1.0"
     exon            11357..11428
                     /gene="LYST"
                     /gene_synonym="CHS; CHS1; Mauve"
                     /inference="alignment:Splign:2.1.0"
     exon            11429..13466
                     /gene="LYST"
                     /gene_synonym="CHS; CHS1; Mauve"
                     /inference="alignment:Splign:2.1.0"
     regulatory      13448..13453
                     /regulatory_class="polyA_signal_sequence"
                     /gene="LYST"
                     /gene_synonym="CHS; CHS1; Mauve"
                     /note="hexamer: AATAAA"
     polyA_site      13466
                     /gene="LYST"
                     /gene_synonym="CHS; CHS1; Mauve"
                     /note="major polyA site"
ORIGIN      
        1 ggcgtcggct cggggttctc cgggagaggg ggagtgcgcg gcggccgcag ctgccacaaa
       61 ccaggtgaag ctttgttcta agaatatttg tttcatctag tttatgagtc caaatgatat
      121 agactgtaaa tgtcacagca gtggtgaaag actgctcggt catgagcacc gacagtaact
      181 cactggcacg tgaatttctg accgatgtca accggctttg caatgcagtg gtccagaggg
      241 tggaggccag ggaggaagaa gaggaggaga cgcacatggc aacccttgga cagtaccttg
      301 tccatggtcg aggatttcta ttacttacca agctaaattc tataattgat caggcattga
      361 catgtagaga agaactcctg actcttcttc tgtctctcct tccactggta tggaagatac
      421 ctgtccaaga agaaaaggca acagatttta acctaccgct ctcagcagat ataatcctga
      481 ccaaagaaaa gaactcaagt tcacaaagat ccactcagga aaaattacat ttagaaggaa
      541 gtgccctgtc tagtcaggtt tctgcaaaag taaatgtttt tcgaaaaagc agacgacagc
      601 gtaaaattac ccatcgctat tctgtaagag atgcaagaaa gacacagctc tccacctcag
      661 attcagaagc caattcagat gaaaaaggca tagcaatgaa taagcataga aggccccatc
      721 tgctgcatca ttttttaaca tcgtttccta aacaagacca ccccaaagct aaacttgacc
      781 gcttagcaac caaagaacag actcctccag atgctatggc tttggaaaat tccagagaga
      841 ttattccaag acaggggtca aacactgaca ttttaagtga gccagctgcc ttgtctgtta
      901 tcagtaacat gaacaattct ccatttgact tatgtcatgt tttgttatct ttattagaaa
      961 aagtttgtaa gtttgacgtt accttgaatc ataattctcc tttagcagcc agtgtagtgc
     1021 ccacactaac tgaattccta gcaggctttg gggactgctg cagtctgagc gacaacttgg
     1081 agagtcgagt agtttctgca ggttggaccg aagaaccggt ggctttgatt caaaggatgc
     1141 tctttcgaac agtgttgcat cttctgtcag tagatgttag tactgcagag atgatgccag
     1201 aaaatcttag gaaaaattta actgaattgc ttagagcagc tttaaaaatt agaatatgcc
     1261 tagaaaagca gcctgaccct tttgcaccaa gacaaaagaa aacactgcag gaggttcagg
     1321 aagattttgt gttttcaaag tatcgtcata gagcccttct tttacctgag cttttggaag
     1381 gagttcttca gattctgatc tgttgtcttc aaagtgcagc ttcaaatccc ttctacttca
     1441 gtcaagccat ggatttggtt caagaattca ttcagcatca tggatttaat ttatttgaaa
     1501 cagcagttct tcaaatggaa tggctggttt taagagatgg agttcctccc gaggcctcag
     1561 agcatttgaa agccctaata aatagtgtga tgaaaataat gagcactgtc aaaaaagtga
     1621 aatcagagca acttcatcat tcgatgtgta caagaaaaag gcacagacga tgtgaatatt
     1681 ctcattttat gcatcatcac cgagatctct caggtcttct ggtttcggct tttaaaaacc
     1741 aggtttccaa aaacccattt gaagagactg cagatggaga tgtttattat cctgagcggt
     1801 gctgttgcat tgcagtgtgt gcccatcagt gcttgcgctt actacagcag gcttccttga
     1861 gcagcacttg tgtccagatc ctatcgggtg ttcataacat tggaatatgc tgttgtatgg
     1921 atcccaaatc tgtaatcatt cctttgctcc atgcttttaa attgccagca ctgaaaaatt
     1981 ttcagcagca tatattgaat atccttaaca aacttatttt ggatcagtta ggaggagcag
     2041 agatatcacc aaaaattaaa aaagcagctt gtaatatttg tactgttgac tctgaccaac
     2101 tagcccaatt agaagagaca ctgcagggaa acttatgtga tgctgaactc tcctcaagtt
     2161 tatccagtcc ttcttacaga tttcaaggga tcctgcccag cagtggatct gaagatttgt
     2221 tgtggaaatg ggatgcttta aaggcttatc agaactttgt ttttgaagaa gacagattac
     2281 atagtataca gattgcaaat cacatttgca atttaatcca gaaaggcaat atagttgttc
     2341 agtggaaatt atataattac atatttaatc ctgtgctcca aagaggagtt gaattagcac
     2401 atcattgtca acacctaagc gttacttcag ctcaaagtca tgtatgtagc catcataacc
     2461 agtgcttgcc tcaggacgtg cttcagattt atgtaaaaac tctgcctatc ctgcttaaat
     2521 ccagggtaat aagagatttg tttttgagtt gtaatggagt aagtcaaata atcgaattaa
     2581 attgcttaaa tggtattcga agtcattctc taaaagcatt tgaaactctg ataatcagcc
     2641 taggggagca acagaaagat gcctcagttc cagatattga tgggatagac attgaacaga
     2701 aggagttgtc ctctgtacat gtgggtactt cttttcatca tcagcaagct tattcagatt
     2761 ctcctcagag tctcagcaaa ttttatgctg gcctcaaaga agcttatcca aagagacgga
     2821 agactgttaa ccaagatgtt catatcaaca caataaacct attcctctgt gtggcttttt
     2881 tatgcgtaag taaagaagca gagtctgaca gggagtcggc caatgactca gaagatactt
     2941 ctggctatga cagcacagcc agcgagcctt taagtcatat gctgccatgt atatctctcg
     3001 agagccttgt cttgccttct cctgaacata tgcaccaagc agcagacatt tggtctatgt
     3061 gtcgttggat ctacatgttg agttcagtgt tccagaaaca gttttatagg cttggtggtt
     3121 tccgagtatg ccataagtta atatttatga taatacagaa actgttcaga agtcacaaag
     3181 aggagcaagg aaaaaaggag ggagatacaa gtgtaaatga aaaccaggat ttaaacagaa
     3241 tttctcaacc taagagaact atgaaggaag atttattatc tttggctata aaaagtgacc
     3301 ccataccatc agaactaggt agtctaaaaa agagtgctga cagtttaggt aaattagagt
     3361 tacagcatat ttcttccata aatgtggaag aagtttcagc tactgaagcc gctcccgagg
     3421 aagcaaagct atttacaagt caagaaagtg agacctcact tcaaagtata cgacttttgg
     3481 aagcccttct ggccatttgt cttcatggtg ccagaactag tcaacagaag atggaattgg
     3541 agttacctaa tcagaacttg tctgtggaaa gtatattatt tgaaatgagg gaccatcttt
     3601 cccagtcaaa ggtgattgaa acacaactag caaagccttt atttgatgcc ctgcttcgag
     3661 ttgccctcgg gaattattca gcagattttg aacataatga tgctatgact gagaagagtc
     3721 atcaatctgc agaagaattg tcatcccagc ctggtgattt ttcagaagaa gctgaggatt
     3781 ctcagtgttg tagttttaaa cttttagttg aagaagaagg ttacgaagca gatagtgaaa
     3841 gcaatcctga agatggcgaa acccaggatg atggggtaga cttaaagtct gaaacagaag
     3901 gtttcagtgc atcaagcagt ccaaatgact tactcgaaaa cctcactcaa ggggaaataa
     3961 tttatcctga gatttgtatg ctggaattaa atttgctttc tgctagtaaa gccaaacttg
     4021 atgtgcttgc ccatgtattt gagagttttt tgaaaattat taggcagaaa gaaaagaatg
     4081 tttttctgct catgcaacag ggaactgtga aaaatctttt aggagggttc ttgagtattt
     4141 taacacagga tgattctgat tttcaagcat gccagagagt attggtggat cttttggtat
     4201 ctttgatgag ttcaagaaca tgttcagaag agctaaccct tcttttgaga atatttctgg
     4261 agaaatctcc ttgtacaaaa attcttcttc tgggtattct gaaaattatt gaaagtgata
     4321 ctactatgag cccttcacag tatctaacct tccctttact gcacgctcca aatttaagca
     4381 acggtgtttc atcacaaaag tatcctggga ttttaaacag taaggccatg ggtttattga
     4441 gaagagcacg agtttcacgg agcaagaaag aggctgatag agagagtttt ccccatcggc
     4501 tgctttcatc ttggcacata gccccagtcc acctgccgtt gctggggcaa aactgctggc
     4561 cacacctatc agaaggtttc agtgtttccc tgtggtttaa tgtggagtgt atccatgaag
     4621 ctgagagtac tacagaaaaa ggaaagaaga taaagaaaag aaacaaatca ttaattttac
     4681 cagatagcag ttttgatggt acagagagcg acagaccaga aggtgcagag tacataaatc
     4741 ctggtgaaag actcatagaa gaaggatgta ttcatataat ttcactggga tccaaagcgt
     4801 tgatgatcca agtgtgggct gatccccaca atgccactct tatctttcgt gtgtgcatgg
     4861 attcaaatga tgacatgaaa gctgttttac tagcacaggt tgaatcacag gagaatattt
     4921 tcctcccaag caaatggcaa catttagtac tcacctactt acagcagccc caagggaaaa
     4981 ggaggattca tgggaaaatc tccatatggg tctctggaca gaggaagcct gatgttactt
     5041 tggattttat gcttccaaga aaaacaagtt tgtcatctga tagcaataaa acattttgca
     5101 tgattggcca ttgtttatca tcccaagaag agtttttgca gttggctgga aaatgggacc
     5161 tgggaaattt gcttctcttc aacggagcta aggttggttc acaagaggcc ttttatctgt
     5221 atgcttgtgg acccaaccat acatctgtaa tgccatgtaa gtatggcaag ccagtcaatg
     5281 actactccaa atatattaat aaagaaattt tgcgatgtga acaaatcaga gaacttttta
     5341 tgaccaagaa agatgtggat attggtctct taattgaaag tctttcagtt gtttatacaa
     5401 cttactgtcc tgctcagtat accatctatg aaccagtgat tagacttaaa ggtcaaatga
     5461 aaacccaact ctctcaaaga cccttcagct caaaagaagt tcagagcatc ttattagaac
     5521 ctcatcatct aaagaatctc caacctactg aatataaaac tattcaaggc attctgcacg
     5581 aaattggtgg aactggcata tttgtttttc tctttgccag ggttgttgaa ctcagtagct
     5641 gtgaagaaac tcaagcatta gcactgcgag ttatactctc attaattaaa tacaaccaac
     5701 aaagagtaca tgaattagaa aattgtaatg gactttctat gattcatcag gtgttgatca
     5761 aacaaaaatg cattgttggg ttttacattt tgaagaccct tcttgaagga tgctgtggtg
     5821 aagatattat ttatatgaat gagaatggag agtttaagtt ggatgtagac tctaatgcta
     5881 taatccaaga tgttaagctg ttagaggaac tattgcttga ctggaagata tggagtaaag
     5941 cagagcaagg tgtttgggaa actttgctag cagctctaga agtcctcatc agagcagatc
     6001 accaccagca gatgtttaat attaagcagt tattgaaagc tcaagtggtt catcactttc
     6061 tactgacttg tcaggttttg caggaataca aagaggggca actcacaccc atgccccgag
     6121 aggtttgtag atcatttgtg aaaattatag cagaagtcct tggatctcct ccagatttgg
     6181 aattattgac aattatcttc aatttccttt tagcagttca ccctcctact aatacttacg
     6241 tttgtcacaa tcccacgaac ttctactttt ctttgcacat agatggcaag atctttcagg
     6301 agaaagtgcg gtcaatcatg tacctgaggc attccagcag tggaggaagg tcccttatga
     6361 gccctggatt tatggtaata agcccatctg gttttactgc ttcaccatat gaaggagaga
     6421 attcctctaa tattattcca caacagatgg ccgcccatat gctgcgttct agaagcctac
     6481 cagcattccc tacttcttca ctactaacgc aatcacaaaa actgactgga agtttgggtt
     6541 gtagtatcga caggttacaa aatattgcag atacttatgt tgccacccaa tcaaagaaac
     6601 aaaattcttt ggggagttcc gacacactga aaaaaggcaa agaggacgca ttcatcagta
     6661 gctgtgagtc tgcaaaaact gtttgtgaaa tggaagctgt cctctcagcc caggtctctg
     6721 tcagtgatgt cccaaaggga gtgctgggat ttccagtggt caaagcagat cataaacagt
     6781 tgggagcaga acccaggtca gaagatgaca gtcctgggga tgagtcctgc ccacgccgac
     6841 ctgattacct aaagggattg gcctccttcc agcgaagcca cagcactatt gcaagccttg
     6901 ggctagcttt tccttcacag aacggatctg cagctgttgg ccgttggcca agtcttgttg
     6961 atagaaacac tgatgattgg gaaaactttg cctattctct tggttatgag ccaaattaca
     7021 accgaactgc aagtgctcac agtgtaactg aagactgttt ggtacctata tgctgtggat
     7081 tatatgaact cctaagtggg gttcttctta tcctgcctga tgttttgctt gaagatgtga
     7141 tggacaagct tattcaagca gatacacttt tggtcctcgt taaccaccca tcaccagcta
     7201 tacaacaagg tgttattaaa ctattagatg catattttgc tagagcatct aaggaacaaa
     7261 aagataaatt tctgaagaat cgtggatttt ccttgctagc caaccagttg tatcttcatc
     7321 gaggaactca agaattgtta gaatgcttca tcgaaatgtt ctttggtcga catattggcc
     7381 ttgatgaaga atttgatctg gaagatgtga gaaacatggg attgtttcag aagtggtctg
     7441 tcattcctat tctgggacta atagagacct ctctatatga caacatactc ttgcataatg
     7501 ctcttttact tcttctccaa attttaaatt cttgttctaa ggtagcagat atgttgctgg
     7561 ataatggtct actctatgtg ttatgtaata cagtagcagc cctgaatgga ttagaaaaga
     7621 acattcccat gagtgaatat aaattgcttg cttgtgatat acagcaactt ttcatagcag
     7681 ttacaattca tgcttgcagt tcctcaggct cacaatattt tagggttatt gaagacctta
     7741 ttgtaatgct tggatatctt caaaatagca aaaacaagag gacacaaaat atggctgttg
     7801 cactacagct tagagttctc caggctgcta tggaatttat aaggaccacc gcaaatcatg
     7861 actctgaaaa cctcacagat tcactccagt caccttctgc tccccatcat gcagtagttc
     7921 aaaagcggaa aagcattgct ggtcctcgaa aatttcccct tgctcaaact gaatcgcttc
     7981 tgatgaaaat gcgttcagtg gcaaatgatg agcttcatgt gatgatgcaa cggagaatga
     8041 gccaagagaa ccctagccaa gcaactgaaa cggaacttgc gcagagacta cagaggctca
     8101 ctgttttagc agtcaacagg attatttatc aagaatttaa ttcagacatt attgacattt
     8161 tgagaactcc agaaaatgta actcaaagca agacctcagt tttccagacc gaaatttctg
     8221 aggaaaatat tcatcatgaa cagtcttctg ttttcaatcc atttcagaaa gaaattttta
     8281 catatctggt agaaggattc aaagtatcta ttggttcaag taaagccagt ggttccaagc
     8341 agcaatggac taaaattctg tggtcttgta aggagacctt ccgaatgcag cttgggagac
     8401 tactagtgca tattttgtcg ccagcccacg ctgcacaaga gagaaagcaa atttttgaaa
     8461 tagttcatga accaaatcat caggaaatac tacgagactg tctcagccca tccctacaac
     8521 atggagccaa gttagttttg tatttgtcag agttgataca taatcaccaa ggtgaattga
     8581 ctgaagaaga gctaggcaca gcagaactgc ttatgaatgc tttgaagtta tgtggtcaca
     8641 agtgcatccc tcccagtgca tcaacaaaag cagaccttat taaaatgatc aaagaggaac
     8701 aaaagaaata tgaaactgaa gaaggagtga ataaagctgc ttggcagaaa acagttaaca
     8761 ataatcaaca aagtctcttt cagcgtctgg attcaaaatc aaaggatata tctaaaatag
     8821 ctgcagatat cacccaggca gtgtctctct cccaaggaaa tgagagaaaa aaggtgatcc
     8881 agcatattag aggaatgtat aaagtagatt tgagtgccag cagacattgg caggaactta
     8941 ttcagcagct gacacatgat agagcagtat ggtatgaccc catctactat ccaacctcat
     9001 ggcagttgga tccaacagaa gggccaaatc gagagaggag acgtttacag agatgttatt
     9061 taactattcc aaataagtat ctccttaggg atagacagaa atcagaagat gttgtcaaac
     9121 caccactctc ttacctgttt gaagacaaaa ctcattcttc tttctcttct actgtcaaag
     9181 acaaagctgc aagtgaatct ataagagtga atcgaagatg catcagtgtt gcaccatcta
     9241 gagagacagc tggtgaattg ttactaggta aatgtggaat gtattttgtg gaagataatg
     9301 cttctgatac agttgaaagt tcgagccttc agggagagtt ggaaccagca tcattttcct
     9361 ggacatatga agaaattaaa gaagttcaca agcgttggtg gcaattgaga gataatgctg
     9421 tagaaatctt tctaacaaat ggcagaacac tcctgttggc atttgataac accaaggttc
     9481 gtgatgatgt ataccacaat atactcacaa ataacctccc taatcttctg gaatatggta
     9541 acatcaccgc tctgacaaat ttatggtata ctgggcaaat tactaatttt gaatatttga
     9601 ctcacttaaa caaacatgct ggccgatcct tcaatgatct catgcagtat cctgtgttcc
     9661 catttatact tgctgactac gttagtgaga cacttgacct caatgatctg ttgatataca
     9721 gaaatctctc taaacctata gctgttcagt ataaagaaaa agaagatcgt tatgtggaca
     9781 catacaagta cttggaggaa gagtaccgca aaggagccag agaagatgac cccatgcctc
     9841 ccgtgcagcc ctatcactat ggctcccact attccaatag cggcactgtg cttcacttcc
     9901 tggtcaggat gcctcctttc actaaaatgt ttttagccta tcaagatcaa agttttgaca
     9961 ttccagacag aacttttcat tctacaaata caacttggcg actctcatct tttgaatcta
    10021 tgactgatgt gaaagaactt atcccagagt ttttctatct tccagagttc ctagttaacc
    10081 gtgaaggttt tgattttggt gtgcgtcaga atggtgaacg ggttaatcac gtcaaccttc
    10141 ccccttgggc gcgtaatgat cctcgtcttt ttatcctcat ccatcggcag gctctagagt
    10201 ctgactacgt gtcgcagaac atctgtcagt ggattgactt ggtgtttggg tataagcaaa
    10261 aggggaaggc ttctgttcaa gcgatcaatg tttttcatcc tgctacatat tttggaatgg
    10321 atgtctctgc agttgaagat ccagttcaga gacgagcgct agaaaccatg ataaaaacct
    10381 acgggcagac tccccgtcag ctgttccaca tggcccatgt gagcagacct ggagccaagc
    10441 tcaatattga aggagagctt ccagctgctg tggggttgct agtgcagttt gctttcaggg
    10501 agacccgaga acaggtcaaa gaaatcacct atccgagtcc tttgtcatgg ataaaaggct
    10561 tgaaatgggg ggaatacgtg ggttccccca gtgctccagt acctgtggtc tgcttcagcc
    10621 agccccacgg agaaagattt ggctctctcc aggctctgcc caccagagca atctgtggtt
    10681 tgtcacggaa tttctgtctt ctgatgacat atagcaagga acaaggtgtg agaagcatga
    10741 acagtacgga cattcagtgg tcagccatcc tgagctgggg atatgctgat aatattttaa
    10801 ggttgaagag taaacaaagt gagcctccag taaactttat tcaaagttca caacagtacc
    10861 aggtgactag ttgtgcttgg gtgcctgaca gttgccagct gtttactgga agcaaatgcg
    10921 gtgtcatcac agcctacaca aacagattta caagcagcac gccatcagaa atagaaatgg
    10981 agactcaaat acatctctat ggtcacacag aagagataac cagcttattt gtttgcaaac
    11041 catacagtat actgataagt gtgagcagag acggaacctg catcatatgg gatttaaaca
    11101 ggttatgcta tgtacaaagt ctggcgggac acaaaagccc tgtcacagct gtctctgcca
    11161 gtgaaacctc aggtgatatt gctactgtgt gtgattcagc tggcggaggc agtgacctca
    11221 gactctggac ggtgaacggg gatctcgttg gacatgtcca ctgcagggag atcatctgtt
    11281 ccgtggcttt ctccaaccag cctgagggag tatctatcaa tgtaatcgct gggggattag
    11341 aaaatggaat tgtaaggtta tggagcacat gggacttaaa gcctgtgaga gaaattacat
    11401 ttcccaaatc aaataagccc atcatcagcc ttacattttc ttgtgatggc caccatttgt
    11461 acacagcaaa cagtgatggg accgtgattg cctggtgtcg gaaggaccag cagcgcttga
    11521 aacagccaat gttctattcc ttccttagca gctatgcagc cgggtgaatg cgaatgaact
    11581 tcacgttctc caaagcactt taactccaaa ctagatttgt tgacttcacc agttttagga
    11641 ggttgaacct aaagaaatgg atgactggac aaaccatcca aataatgata aagtctattc
    11701 atctgcacaa aattctgaag agtcacatga tcctaagagg aaagttctgt tctattttag
    11761 tgataatctg gaagattgtg tcaatatgca ctagccaaca agttttaagc ctcgcatggt
    11821 acattaaaat gatattctta aaattttttc ccaccaaggt attccaaaga aaatattaag
    11881 gtctcccctt tttctatgat tccaaaagga ccagtagaat ttaaattggt tggttgattg
    11941 tttatataaa acacactaaa attatatttt aaaagtttac tgccatgaaa tactcctccc
    12001 accacacaca catgctccaa aagaggaaag aaaaaaagat aatttttagg acttgataat
    12061 tgctttcttt gagaagcaaa ttattcagta ggtgcctctg taccaaatat tttatggaat
    12121 atctaaatac taaaataaac tatgaatgaa tctcaaaatt aggcagtttt tgccagttgc
    12181 tttcttagct caaaggagaa ccagaatttt tttgacagcc acaaacaaga atacaggtat
    12241 cttggatttc agacacattc tgtttcttca taaaaatttt acttaaaatc tgtaacgcta
    12301 gatattgact atccttagtt gagtcactga ggtttaaaca caatggtaag tcttaaagtc
    12361 tgctatttac agagcattga atctgtacca atttgcaata gaaagccttc agtatgcaag
    12421 aagtttgcat gggtattaag aacacagcct aaataaggca tttgatctaa tctgcaggaa
    12481 gaattttctt ccccaaaaca gaattataaa agcttacttt aaacaggagg cagaataatt
    12541 cttttaggaa accatttcat tctgtttcta ctaacctata ccatctgaga attctaggaa
    12601 gaataataaa atctcgtgta ttccacagca aacttacata ccataaagac agaattccta
    12661 aacatcttgg agccgtctgt ctctcccata tgatggctgt ctgtatattt ttacttgggg
    12721 tgctgcttta ttggctttga aaacactgtc agataagctc agtaatatgt taccatggga
    12781 taaaaatatg tatccctgcc taagaataac ttgtgcattt gttatggaaa tttaattcat
    12841 atggtgttta cagtactact tttgtaactt ccagactttc taaaacattc tgcttaaaaa
    12901 ccatataaaa tataattcca aagtctctgc tgtcaagata gattcgagag aaagcacgtg
    12961 gccatgtatg ctttaacctt aaactgcata cacatgtagt gatacctagg ctgcatttag
    13021 atcaccgtgt gctcaggcca ggtgtgaatc ctgaggtcca tggaggtgca gagatgagat
    13081 tactcctatt cacgttgaag tgatttgctt tgttaacaaa aaattgcagc tattgtctag
    13141 ctttcatttt tttactgaga actttaaatt agtcccctat tagaataggg ttgctactca
    13201 tcttttttta aaaaccgaat ttcatcattt atctaaagag aaaatatgca gaataactgg
    13261 tcttgttaag agtgcaatat tatattttta tgtaaaaata aaaattaatt tggggggatt
    13321 atttattcag catgaaacct aatatgtata tgtttgaaat acttcataat gtgcatgttg
    13381 tagcaaacat ttctgtaaat tatcacaagc tctgttacct ttatatacac tgcctcttca
    13441 atttggaaat aaatttcata aaaata
//
