LOCUS       NM_015565               7677 bp    mRNA    linear   PRI 07-FEB-2025
DEFINITION  Homo sapiens listerin E3 ubiquitin protein ligase 1 (LTN1),
            transcript variant 1, mRNA.
ACCESSION   NM_015565
VERSION     NM_015565.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 7677)
  AUTHORS   Qin,F., Cai,B., Wang,P., Cao,R., Zhang,Y., Wen,H., Zheng,Y.,
            Zhao,W., Gao,C. and Liu,B.
  TITLE     LTN1 promotes RLR degradation to inhibit immune response to RNA
            virus through the ESCRT pathway
  JOURNAL   Autophagy 20 (6), 1270-1285 (2024)
   PUBMED   38060409
  REMARK    GeneRIF: LTN1 promotes RLR degradation to inhibit immune response
            to RNA virus through the ESCRT pathway.
REFERENCE   2  (bases 1 to 7677)
  AUTHORS   Farhang,S., Sabaie,H., Gharesouran,J., Asadi,M.R., Arsang-Jang,S.,
            Ghafouri-Fard,S., Taheri,M. and Rezazadeh,M.
  TITLE     Expression Analysis of Ermin and Listerin E3 Ubiquitin Protein
            Ligase 1 Genes in the Periphery of Patients with Schizophrenia
  JOURNAL   J Mol Neurosci 72 (2), 246-254 (2022)
   PUBMED   34676516
  REMARK    GeneRIF: Expression Analysis of Ermin and Listerin E3 Ubiquitin
            Protein Ligase 1 Genes in the Periphery of Patients with
            Schizophrenia.
REFERENCE   3  (bases 1 to 7677)
  AUTHORS   Matsuo,Y., Ikeuchi,K., Saeki,Y., Iwasaki,S., Schmidt,C.,
            Udagawa,T., Sato,F., Tsuchiya,H., Becker,T., Tanaka,K.,
            Ingolia,N.T., Beckmann,R. and Inada,T.
  TITLE     Ubiquitination of stalled ribosome triggers ribosome-associated
            quality control
  JOURNAL   Nat Commun 8 (1), 159 (2017)
   PUBMED   28757607
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 7677)
  AUTHORS   von der Malsburg,K., Shao,S. and Hegde,R.S.
  TITLE     The ribosome quality control pathway can access nascent
            polypeptides stalled at the Sec61 translocon
  JOURNAL   Mol Biol Cell 26 (12), 2168-2180 (2015)
   PUBMED   25877867
  REMARK    GeneRIF: E3 ligase listerin accesses nascent polypeptides stalled
            at the Sec61 translocon via a gap in the ribosome-translocon
            junction near the Sec61 lateral gate.
REFERENCE   5  (bases 1 to 7677)
  AUTHORS   Shao,S., Brown,A., Santhanam,B. and Hegde,R.S.
  TITLE     Structure and assembly pathway of the ribosome quality control
            complex
  JOURNAL   Mol Cell 57 (3), 433-444 (2015)
   PUBMED   25578875
  REMARK    GeneRIF: Results show that Listerin specificity for nascent
            chain-60S complexes depends on nuclear export mediator factor.
REFERENCE   6  (bases 1 to 7677)
  AUTHORS   Li,S., Wang,L., Berman,M., Kong,Y.Y. and Dorf,M.E.
  TITLE     Mapping a dynamic innate immunity protein interaction network
            regulating type I interferon production
  JOURNAL   Immunity 35 (3), 426-440 (2011)
   PUBMED   21903422
  REMARK    Erratum:[Immunity. 2011 Oct 28;35(4):647-8]
REFERENCE   7  (bases 1 to 7677)
  AUTHORS   Oshikawa,M., Tsutsui,C., Ikegami,T., Fuchida,Y., Matsubara,M.,
            Toyama,S., Usami,R., Ohtoko,K. and Kato,S.
  TITLE     Full-length transcriptome analysis of human retina-derived cell
            lines ARPE-19 and Y79 using the vector-capping method
  JOURNAL   Invest Ophthalmol Vis Sci 52 (9), 6662-6670 (2011)
   PUBMED   21697133
  REMARK    Publication Status: Online-Only
REFERENCE   8  (bases 1 to 7677)
  AUTHORS   Bengtson,M.H. and Joazeiro,C.A.
  TITLE     Role of a ribosome-associated E3 ubiquitin ligase in protein
            quality control
  JOURNAL   Nature 467 (7314), 470-473 (2010)
   PUBMED   20835226
REFERENCE   9  (bases 1 to 7677)
  AUTHORS   Chu,J., Hong,N.A., Masuda,C.A., Jenkins,B.V., Nelms,K.A.,
            Goodnow,C.C., Glynne,R.J., Wu,H., Masliah,E., Joazeiro,C.A. and
            Kay,S.A.
  TITLE     A mouse forward genetics screen identifies LISTERIN as an E3
            ubiquitin ligase involved in neurodegeneration
  JOURNAL   Proc Natl Acad Sci U S A 106 (7), 2097-2103 (2009)
   PUBMED   19196968
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            DB452437.1, BC140790.1, AB018257.2 and BF059116.1.
            
            On Sep 13, 2018 this sequence version replaced NM_015565.2.
            
            Summary: Like most RING finger proteins, LTN1 functions as an E3
            ubiquitin ligase (Chu et al., 2009 [PubMed 19196968]).[supplied by
            OMIM, Nov 2010].
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR11853565.19104.1,
                                           SRR11853559.21293.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000361371.10/ ENSP00000354977.4
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-8                 DB452437.1         83-90
            9-6769              BC140790.1         1-6761
            6770-7269           AB018257.2         6740-7239
            7270-7677           BF059116.1         1-408               c
FEATURES             Location/Qualifiers
     source          1..7677
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="21"
                     /map="21q21.3"
     gene            1..7677
                     /gene="LTN1"
                     /gene_synonym="C21orf10; C21orf98; RNF160; ZNF294"
                     /note="listerin E3 ubiquitin protein ligase 1"
                     /db_xref="GeneID:26046"
                     /db_xref="HGNC:HGNC:13082"
                     /db_xref="MIM:613083"
     exon            1..114
                     /gene="LTN1"
                     /gene_synonym="C21orf10; C21orf98; RNF160; ZNF294"
                     /inference="alignment:Splign:2.1.0"
     CDS             73..5373
                     /gene="LTN1"
                     /gene_synonym="C21orf10; C21orf98; RNF160; ZNF294"
                     /EC_number="2.3.2.27"
                     /note="isoform 1 is encoded by transcript variant 1; ring
                     finger protein 160; zinc finger protein 294; E3
                     ubiquitin-protein ligase listerin; RING-type E3 ubiquitin
                     transferase listerin"
                     /codon_start=1
                     /product="E3 ubiquitin-protein ligase listerin isoform 1"
                     /protein_id="NP_056380.3"
                     /db_xref="CCDS:CCDS33527.3"
                     /db_xref="GeneID:26046"
                     /db_xref="HGNC:HGNC:13082"
                     /db_xref="MIM:613083"
                     /translation="MGGKNKQRTKGNLRPSNSGRAAELLAKEQGTVPGFIGFGTSQSD
                     LGYVPAIQGAEEIDSLVDSDFRMVLRKLSKKDVTTKLKAMQEFGTMCTERDTETVKGV
                     LPYWPRIFCKISLDHDRRVREATQQAFEKLILKVKKQLAPYLKSLMGYWLMAQCDTYT
                     PAAFAAKDAFEAAFPPSKQPEAIAFCKDEITSVLQDHLIKETPDTLSDPQTVPEEERE
                     AKFYRVVTCSLLALKRLLCLLPDNELDSLEEKFKSLLSQNKFWKYGKHSVPQIRSAYF
                     ELVSALCQRIPQLMKEEASKVSPSVLLSIDDSDPIVCPALWEAVLYTLTTIEDCWLHV
                     NAKKSVFPKLSTVIREGGRGLATVIYPYLLPFISKLPQSITNPKLDFFKNFLTSLVAG
                     LSTERTKTSSLESSAVISAFFECLRFIMQQNLGEEEIEQMLVNDQLIPFIDAVLKDPG
                     LQHGQLFNHLAETLSSWEAKADTEKDEKTAHNLENVLIHFWERLSEICVAKISEPEAD
                     VESVLGVSNLLQVLQKPKSSLKSSKKKNGKVRFADEILESNKENEKCVSSEGEKIEGW
                     ELTTEPSLTHNSSGLLSPLRKKPLEDLVCKLADISINYVNERKSEQHLRFLSTLLDSF
                     SSSRVFKMLLGDEKQSIVQAKPLEIAKLVQKNPAVQFLYQKLIGWLNEDQRKDFGFLV
                     DILYSALRCCDNDMERKKVLDDLTKVDLKWNSLLKIIEKACPSSDKHALVTPWLKGDI
                     LGEKLVNLADCLCNEDLESRVSSESHFSERWTLLSLVLSQHVKNDYLIGDVYVERIIV
                     RLHETLFKTKKLSEAESSDSSVSFICDVAYNYFSSAKGCLLMPSSEDLLLTLFQLCAQ
                     SKEKTHLPDFLICKLKNTWLSGVNLLVHQTDSSYKESTFLHLSALWLKNQVQASSLDI
                     NSLQVLLSAVDDLLNTLLESEDSYLMGVYIGSVMPNDSEWEKMRQSLPMQWLHRPLLE
                     GRLSLNYECFKTDFKEQDIKTLPSHLCTSALLSKMVLIALRKETVLENNELEKIIAEL
                     LYSLQWCEELDNPPIFLIGFCEILQKMNITYDNLRVLGNTSGLLQLLFNRSREHGTLW
                     SLIIAKLILSRSISSDEVKPHYKRKESFFPLTEGNLHTIQSLCPFLSKEEKKEFSAQC
                     IPALLGWTKKDLCSTNGGFGHLAIFNSCLQTKSIDDGELLHGILKIIISWKKEHEDIF
                     LFSCNLSEASPEVLGVNIEIIRFLSLFLKYCSSPLAESEWDFIMCSMLAWLETTSENQ
                     ALYSIPLVQLFACVSCDLACDLSAFFDSTTLDTIGNLPVNLISEWKEFFSQGIHSLLL
                     PILVTVTGENKDVSETSFQNAMLKPMCETLTYISKEQLLSHKLPARLVADQKTNLPEY
                     LQTLLNTLAPLLLFRARPVQIAVYHMLYKLMPELPQYDQDNLKSYGDEEEEPALSPPA
                     ALMSLLSIQEDLLENVLGCIPVGQIVTIKPLSEDFCYVLGYLLTWKLILTFFKAASSQ
                     LRALYSMYLRKTKSLNKLLYHLFRLMPENPTYAETAVEVPNKDPKTFFTEELQLSIRE
                     TTMLPYHIPHLACSVYHMTLKDLPAMVRLWWNSSEKRVFNIVDRFTSKYVSSVLSFQE
                     ISSVQTSTQLFNGMTVKARATTREVMATYTIEDIVIELIIQLPSNYPLGSIIVESGKR
                     VGVAVQQWRNWMLQLSTYLTHQNGSIMEGLALWKNNVDKRFEGVEDCMICFSVIHGFN
                     YSLPKKACRTCKKKFHSACLYKWFTSSNKSTCPLCRETFF"
     exon            115..318
                     /gene="LTN1"
                     /gene_synonym="C21orf10; C21orf98; RNF160; ZNF294"
                     /inference="alignment:Splign:2.1.0"
     exon            319..417
                     /gene="LTN1"
                     /gene_synonym="C21orf10; C21orf98; RNF160; ZNF294"
                     /inference="alignment:Splign:2.1.0"
     exon            418..648
                     /gene="LTN1"
                     /gene_synonym="C21orf10; C21orf98; RNF160; ZNF294"
                     /inference="alignment:Splign:2.1.0"
     exon            649..701
                     /gene="LTN1"
                     /gene_synonym="C21orf10; C21orf98; RNF160; ZNF294"
                     /inference="alignment:Splign:2.1.0"
     exon            702..882
                     /gene="LTN1"
                     /gene_synonym="C21orf10; C21orf98; RNF160; ZNF294"
                     /inference="alignment:Splign:2.1.0"
     exon            883..1056
                     /gene="LTN1"
                     /gene_synonym="C21orf10; C21orf98; RNF160; ZNF294"
                     /inference="alignment:Splign:2.1.0"
     exon            1057..1247
                     /gene="LTN1"
                     /gene_synonym="C21orf10; C21orf98; RNF160; ZNF294"
                     /inference="alignment:Splign:2.1.0"
     exon            1248..1383
                     /gene="LTN1"
                     /gene_synonym="C21orf10; C21orf98; RNF160; ZNF294"
                     /inference="alignment:Splign:2.1.0"
     exon            1384..2193
                     /gene="LTN1"
                     /gene_synonym="C21orf10; C21orf98; RNF160; ZNF294"
                     /inference="alignment:Splign:2.1.0"
     exon            2194..2235
                     /gene="LTN1"
                     /gene_synonym="C21orf10; C21orf98; RNF160; ZNF294"
                     /inference="alignment:Splign:2.1.0"
     exon            2236..2425
                     /gene="LTN1"
                     /gene_synonym="C21orf10; C21orf98; RNF160; ZNF294"
                     /inference="alignment:Splign:2.1.0"
     exon            2426..2665
                     /gene="LTN1"
                     /gene_synonym="C21orf10; C21orf98; RNF160; ZNF294"
                     /inference="alignment:Splign:2.1.0"
     exon            2666..2819
                     /gene="LTN1"
                     /gene_synonym="C21orf10; C21orf98; RNF160; ZNF294"
                     /inference="alignment:Splign:2.1.0"
     exon            2820..2964
                     /gene="LTN1"
                     /gene_synonym="C21orf10; C21orf98; RNF160; ZNF294"
                     /inference="alignment:Splign:2.1.0"
     exon            2965..3151
                     /gene="LTN1"
                     /gene_synonym="C21orf10; C21orf98; RNF160; ZNF294"
                     /inference="alignment:Splign:2.1.0"
     exon            3152..3311
                     /gene="LTN1"
                     /gene_synonym="C21orf10; C21orf98; RNF160; ZNF294"
                     /inference="alignment:Splign:2.1.0"
     exon            3312..3416
                     /gene="LTN1"
                     /gene_synonym="C21orf10; C21orf98; RNF160; ZNF294"
                     /inference="alignment:Splign:2.1.0"
     exon            3417..3559
                     /gene="LTN1"
                     /gene_synonym="C21orf10; C21orf98; RNF160; ZNF294"
                     /inference="alignment:Splign:2.1.0"
     exon            3560..3695
                     /gene="LTN1"
                     /gene_synonym="C21orf10; C21orf98; RNF160; ZNF294"
                     /inference="alignment:Splign:2.1.0"
     exon            3696..3840
                     /gene="LTN1"
                     /gene_synonym="C21orf10; C21orf98; RNF160; ZNF294"
                     /inference="alignment:Splign:2.1.0"
     exon            3841..4054
                     /gene="LTN1"
                     /gene_synonym="C21orf10; C21orf98; RNF160; ZNF294"
                     /inference="alignment:Splign:2.1.0"
     exon            4055..4292
                     /gene="LTN1"
                     /gene_synonym="C21orf10; C21orf98; RNF160; ZNF294"
                     /inference="alignment:Splign:2.1.0"
     exon            4293..4367
                     /gene="LTN1"
                     /gene_synonym="C21orf10; C21orf98; RNF160; ZNF294"
                     /inference="alignment:Splign:2.1.0"
     exon            4368..4554
                     /gene="LTN1"
                     /gene_synonym="C21orf10; C21orf98; RNF160; ZNF294"
                     /inference="alignment:Splign:2.1.0"
     exon            4555..4726
                     /gene="LTN1"
                     /gene_synonym="C21orf10; C21orf98; RNF160; ZNF294"
                     /inference="alignment:Splign:2.1.0"
     exon            4727..4947
                     /gene="LTN1"
                     /gene_synonym="C21orf10; C21orf98; RNF160; ZNF294"
                     /inference="alignment:Splign:2.1.0"
     exon            4948..5142
                     /gene="LTN1"
                     /gene_synonym="C21orf10; C21orf98; RNF160; ZNF294"
                     /inference="alignment:Splign:2.1.0"
     exon            5143..5310
                     /gene="LTN1"
                     /gene_synonym="C21orf10; C21orf98; RNF160; ZNF294"
                     /inference="alignment:Splign:2.1.0"
     exon            5311..7677
                     /gene="LTN1"
                     /gene_synonym="C21orf10; C21orf98; RNF160; ZNF294"
                     /inference="alignment:Splign:2.1.0"
     regulatory      6003..6008
                     /regulatory_class="polyA_signal_sequence"
                     /gene="LTN1"
                     /gene_synonym="C21orf10; C21orf98; RNF160; ZNF294"
                     /note="hexamer: ATTAAA"
     polyA_site      6021
                     /gene="LTN1"
                     /gene_synonym="C21orf10; C21orf98; RNF160; ZNF294"
     regulatory      7651..7656
                     /regulatory_class="polyA_signal_sequence"
                     /gene="LTN1"
                     /gene_synonym="C21orf10; C21orf98; RNF160; ZNF294"
                     /note="hexamer: AATAAA"
     polyA_site      7677
                     /gene="LTN1"
                     /gene_synonym="C21orf10; C21orf98; RNF160; ZNF294"
                     /note="major polyA site"
ORIGIN      
        1 agaagccgga agttgtgtcc cggacgtgtc aaccggggtc tgagtgctca gagtacagct
       61 gcaaccgcga ccatgggcgg gaagaacaag cagcgaacta aagggaacct gaggccttca
      121 aacagtggcc gagctgcaga actccttgcc aaagaacagg gaacagtgcc tggatttatt
      181 ggttttggaa catctcagag tgacctaggc tatgttcctg ctattcaagg agctgaagaa
      241 attgacagtc ttgtagattc tgatttccga atggtgctgc ggaaactttc aaagaaagat
      301 gtcaccacaa aattaaaagc tatgcaggaa tttggaacca tgtgtacaga gagagacaca
      361 gaaactgtga aaggagttct tccatattgg ccaagaattt tttgcaaaat ttcacttgat
      421 catgaccgtc gcgtccgaga agccacacaa caagcttttg aaaaacttat ccttaaagta
      481 aagaaacagt tggctcccta cttaaaaagt ttaatgggat attggctaat ggctcagtgt
      541 gatacttaca caccagctgc gtttgcagca aaagatgcat ttgaagcggc ttttcctcca
      601 agcaagcaac ctgaagccat agcattttgt aaggatgaaa ttacaagtgt gctgcaggat
      661 catcttataa aagaaacacc tgatacactc agtgacccgc aaactgttcc agaggaagaa
      721 agagaagcta aattctaccg ggttgtaact tgttccttat tggcattaaa gagattactt
      781 tgccttttac ctgataatga gcttgattct ctggaggaga aatttaagtc tcttttatca
      841 cagaataagt tttggaagta tggaaaacac agtgtacctc agattcgctc agcttatttt
      901 gagttagtct ctgcattgtg ccagcgcatt ccacagttga tgaaagagga agcatccaaa
      961 gtgagcccat cagttctact tagcattgat gacagtgacc caattgtctg cccagctctc
     1021 tgggaagctg tactctatac acttacaact attgaggact gttggcttca tgtaaatgca
     1081 aaaaagagtg tgtttcccaa gctatcaact gtgattcgtg aaggtggtcg gggtctagct
     1141 actgtcatat atccttacct tctgccattc atcagcaagc tccctcagtc catcacaaat
     1201 ccaaagttgg atttcttcaa aaatttcctc acgtctctag ttgctgggct gtcaacagag
     1261 agaactaaaa ccagctcttt agagtcctcg gcagtaatat ctgctttttt tgaatgctta
     1321 cgttttataa tgcagcaaaa cttaggtgag gaagagattg aacagatgct cgtcaatgat
     1381 cagttgatcc cttttattga tgcagttctc aaagacccag gattgcaaca tgggcagcta
     1441 tttaaccatt tagcagaaac tctaagttcc tgggaagcca aagcagacac ggaaaaagat
     1501 gaaaaaacag ctcacaactt ggagaacgta ctgatacatt tctgggaaag actgtcagag
     1561 atctgtgttg cgaaaatcag tgagccagaa gctgatgttg agtccgtttt gggtgtatct
     1621 aacctattac aggtgcttca gaagccgaag agctcattga agtcaagtaa aaaaaaaaat
     1681 ggtaaggtta gatttgctga tgagatactt gaaagcaata aagagaatga aaaatgtgta
     1741 tcttcagaag gagagaagat tgaaggctgg gaattaacaa ctgaaccttc tctcactcat
     1801 aattcttcag gccttttgtc tcctctaagg aaaaaacctt tggaagactt agtctgtaaa
     1861 ctcgcagata taagtattaa ttatgtcaat gaacgaaagt cagagcaaca tctaaggttt
     1921 ctttctactc tgcttgactc cttttcttca agccgagtat ttaaaatgct acttggtgat
     1981 gaaaaacaga gtattgtcca agccaaacct cttgaaatag ccaagcttgt acaaaaaaat
     2041 cctgcggtgc agtttttata ccagaaactg ataggttggc taaatgaaga tcaaaggaag
     2101 gattttggtt tcctggtgga cattttgtac agtgctctcc ggtgctgtga caatgatatg
     2161 gaaagaaaaa aagtcttgga tgatctaacc aaggtggact tgaaatggaa ttctcttctt
     2221 aagattattg aaaaggcatg tcctagttca gataaacatg ctttagtaac tccttggctc
     2281 aaaggcgata tccttggtga gaaattggtc aacttggcag attgtctttg taatgaggac
     2341 ttggaatcca gggtatcttc agaatctcac ttctcagaaa gatggactct tctaagcttg
     2401 gtattatccc aacatgttaa aaatgattac ttgattggag acgtatatgt tgaaagaatc
     2461 attgttagac ttcatgaaac tttattcaaa acaaagaaat tatcagaagc tgaaagcagt
     2521 gactcatcag tgtcttttat ctgtgatgtg gcctataact atttcagctc agcgaaagga
     2581 tgcttgctaa tgccatcatc tgaagattta ttattaactc tctttcagtt atgtgctcag
     2641 agcaaagaaa aaacacattt gccagatttt cttatctgta aactgaaaaa tacttggctc
     2701 tctggtgtaa atttattggt tcatcaaact gacagttcat ataaagagag taccttccta
     2761 catttgtctg ctctgtggct gaagaaccaa gttcaggctt catctttgga tatcaacagt
     2821 ctccaagtcc tcttgtctgc tgttgatgat ttgctaaata cacttctaga gagtgaagat
     2881 tcttatctta tgggagttta tattggaagt gtaatgccga acgacagtga atgggaaaag
     2941 atgaggcagt ctcttcctat gcagtggtta catagacctc ttttagaggg aagattgagt
     3001 ttgaattatg aatgtttcaa aacagatttt aaggaacagg acataaagac acttcccagc
     3061 catttgtgta cttcagcatt attgagcaaa atggtcttaa ttgcactgag aaaggaaaca
     3121 gtcttagaaa ataatgagct tgagaaaata attgcagaac tgctttattc actgcagtgg
     3181 tgtgaagaat tagataaccc acctattttt ctaattggat tttgtgaaat acttcaaaaa
     3241 atgaatatta cgtatgataa cttacgtgta cttggtaata cgtcgggcct tttgcagctg
     3301 ttatttaaca ggtccagaga acatggcaca ctgtggtctc ttattattgc taagttgatc
     3361 ctttcccgaa gcatttcatc tgatgaagta aaaccacatt ataagagaaa agaaagtttt
     3421 tttccactaa ctgaaggcaa tttgcatacc attcaaagtc tatgtccatt tttgtcaaaa
     3481 gaagaaaaga aagaatttag tgctcaatgt atacctgctc ttttgggctg gactaagaaa
     3541 gatctttgca gcactaatgg aggttttgga catcttgcca ttttcaattc ttgtctgcaa
     3601 accaaaagta tagatgatgg agagctatta catggaatat taaaaatcat aatatcctgg
     3661 aagaaagagc atgaagatat ttttcttttc agttgtaatc tatcagaagc aagtccagag
     3721 gtactgggtg taaatataga aataatccgg tttctttccc tatttctgaa atactgctca
     3781 tcccctttgg cagagagtga gtgggacttc atcatgtgct ccatgttggc ttggttggag
     3841 acaacaagtg agaatcaggc attgtattct attccacttg tgcaactgtt tgcctgtgtc
     3901 agctgtgatt tggcctgtga cctcagtgct ttctttgatt ccacaactct ggataccatt
     3961 ggcaatcttc ctgtaaatct aatcagtgaa tggaaagaat ttttttccca aggcatccac
     4021 agtttgcttt tacctatttt ggtgactgtt acaggagaaa acaaagatgt gtctgaaaca
     4081 tcctttcaga atgcaatgct gaaacccatg tgtgaaacat taacgtatat ctcaaaggaa
     4141 cagctattga gtcacaaact tcctgcaaga ttagttgctg accaaaaaac aaacttacca
     4201 gaatatctcc agactttgtt aaatacattg gccccattac tcctcttcag agctaggcct
     4261 gtgcaaattg ctgtttatca tatgctatac aaattgatgc ctgaattacc acagtatgat
     4321 caggataatc taaagtcata cggagatgaa gaagaagagc cagccttgtc accaccagca
     4381 gcactgatgt ctcttcttag cattcaagag gacttactag aaaatgtttt ggggtgtatt
     4441 cctgttggac agatagttac tattaaacca ctgagtgaag acttctgtta tgttctggga
     4501 taccttctca cttggaaatt aatactaact ttcttcaaag ctgcatcatc acagcttcgg
     4561 gctttgtatt ccatgtatct tcggaaaaca aagagtttga ataaattgct ctatcacctg
     4621 ttcaggctta tgccagaaaa tccaacctat gcagagacag cagttgaggt cccaaataag
     4681 gaccctaaaa cattctttac tgaggagctc cagctgagta ttagagaaac aacaatgctt
     4741 ccataccaca ttccacactt ggcttgttca gtctatcata tgacattaaa agacttgcct
     4801 gccatggtta ggttgtggtg gaatagcagt gagaagcgtg ttttcaatat tgtggataga
     4861 tttacaagca agtatgtcag cagtgttctt tcttttcaag aaatatcttc tgtacaaaca
     4921 agtacacaac tatttaatgg catgacggtt aaagctcgag ctactactcg agaggtaatg
     4981 gctacttata ctattgagga catagttatt gaacttataa tacaactgcc ttcaaattat
     5041 ccactgggtt caataatagt agaaagtggg aaaagagtag gagtagctgt tcagcagtgg
     5101 cggaactgga tgctgcagtt aagcacttac ctcacccatc agaatggaag tattatggaa
     5161 ggcttagctt tatggaaaaa taacgtagac aaacgttttg agggtgttga agattgcatg
     5221 atctgtttct cagtcattca cggtttcaac tattcccttc ccaaaaaagc ctgtagaaca
     5281 tgcaagaaaa aattccattc agcctgcttg tacaaatggt ttacatctag caacaaatcc
     5341 acttgtccac tgtgtcgtga gacgtttttc tgagattttt ttcactggaa gggatccctg
     5401 aagtacatca aacaaaggca ttggatttgg atccgtctga aagtgtggat gtggggaagc
     5461 cagtgagcat tacttttaaa taggaccttc tctggaaaat tattttggtt aatgtaataa
     5521 tcctaaaatc aggtttactt aactttagat tgctttgtaa atgtttggaa actttctttt
     5581 acaaaagaat attacatatt tgagagaaaa tatttctatt aatggtttaa tttctttgta
     5641 tatttaaaaa taaatatgaa aaacccctaa attacagaat tggaatttgt gaaaagattg
     5701 cacaactata ttattgataa acccattccg tttgttgatc tgtgttttag aatagtccaa
     5761 gtgaagcaaa acgaactgag agagctgatt tacatctata gatattttaa gccttattta
     5821 ttagttatga caataaatta gtattctgat atttgtagat tattttaatg ataaaaatga
     5881 cactaagcta ttaatacggc ttgtcttaag atatctggag gcatgctctg aaatcctata
     5941 taaaagagtt taaactgaac tttaaggtgc ctccatttat caagggttat gaaactcaga
     6001 aaattaaatt cttgatgctg acctttttgc ttgtgggaga attatttcta tatattagtg
     6061 aaatttgaat tgtaaacttg tcagtgaatt tatcactctg agcgagtctc tagagggtga
     6121 gaatttgaga gaaatagtaa tttgattatt tcattcatga agcggagtgg tagttatcct
     6181 tctattgaga gggaagaaat aaatgagtat tactgaaact taaagatcag tggaaaaaaa
     6241 aaacatttaa aaatgttgta ggagactttc aattttggaa gcaaactgag ctattcttac
     6301 cagccaaagt tatactaaat aagagacttg ggggagcaaa tgtttttcag ccttcagaaa
     6361 tgaaagttaa ggattttagc actaggtaaa attcagtata ataggctgaa gtggagtaag
     6421 tgaaaacctg ccttttgcca ctcttaaaaa ttgtgcccaa aatataaaag tgtggaactt
     6481 tagaacttgg aataatttta ttgcagtctt ccattacatg gaaatagcat atctaatatc
     6541 taggttactt gagagaccag ctaatcatct ctgttgcaca tgtttaattg gcaaaaagca
     6601 attcatgata aataaaatta ctgctttcca tctactgggt aaaatgacta ttgaaatagt
     6661 atgaatgtgg tcagaggatt atagttgaga gtgaagtact atgtgtgagt tatagatctc
     6721 tcgaattata tttatagatg cagtgtcctg cccagttttg tttgcctcct acattttact
     6781 gtaaaatatt tattgtcttc tagccttgag cctctgaggg tcagtaagtg aagtacagat
     6841 agcaaaattt tactacctcg ttaacccttt tcttaaaata ttctctatct ttctatgtct
     6901 ctttctgaca tagtaatccc aaaggattgt gttactcccc gtgaaagtta ttacttttcc
     6961 tttaaaaatg gttttataat aagactgttt aaaacctttc cagtattggt acatcttggc
     7021 ttctggccca aaccccaagc agaaaagaaa atggaataat ggagcattgt ttttccacat
     7081 tagtattagg gcatcagatt cttggtgaaa cactataatt aagtagttat aattaaataa
     7141 ctgttcttca tacttaggac tcttaataca tttctttaag acttgtaagt ataattgtaa
     7201 agcttgttaa ctgtttatat actaaagaaa aagctttaca agataagact gaaattcaga
     7261 ataccacatc tgtacagcgt tgacaactct aactaaaagg attacagccc ttaaaatttt
     7321 agcttaagga cattttaaaa ttgcatgaga ttagcaaaat tattaattta aaaccccaaa
     7381 gtaaattgca aattttaaaa tttatttttg actcttttat cacatgctcc aatgtatgta
     7441 cataagtgtc tgtggcttaa ataatatgtt gttatttgat acatttttgt ggtgcaagta
     7501 attaatgtta ttatttgatt gttataccat atgtgcccat taaaattctt tttaattaaa
     7561 aatatttcaa atatgcagaa aaataataat ataaatgccc ttgtaccctt cacatactat
     7621 tttgccatat ttgcttctga tttttaaata aataaaatgt tactcttttg tacttct
//
