LOCUS       NM_145867                801 bp    mRNA    linear   PRI 08-JUN-2024
DEFINITION  Homo sapiens leukotriene C4 synthase (LTC4S), mRNA.
ACCESSION   NM_145867
VERSION     NM_145867.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 801)
  AUTHORS   Choi,Y., Sim,S., Lee,D.H., Lee,H.R., Ban,G.Y., Shin,Y.S., Kim,Y.K.
            and Park,H.S.
  TITLE     Effect of TGF-beta1 on eosinophils to induce cysteinyl leukotriene
            E4 production in aspirin-exacerbated respiratory disease
  JOURNAL   PLoS One 16 (8), e0256237 (2021)
   PUBMED   34437574
  REMARK    GeneRIF: Effect of TGF-beta1 on eosinophils to induce cysteinyl
            leukotriene E4 production in aspirin-exacerbated respiratory
            disease.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 801)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   3  (bases 1 to 801)
  AUTHORS   Dalli,J., Vlasakov,I., Riley,I.R., Rodriguez,A.R., Spur,B.W.,
            Petasis,N.A., Chiang,N. and Serhan,C.N.
  TITLE     Maresin conjugates in tissue regeneration biosynthesis enzymes in
            human macrophages
  JOURNAL   Proc Natl Acad Sci U S A 113 (43), 12232-12237 (2016)
   PUBMED   27791009
REFERENCE   4  (bases 1 to 801)
  AUTHORS   Ahmad,S., Ytterberg,A.J., Thulasingam,M., Tholander,F., Bergman,T.,
            Zubarev,R., Wetterholm,A., Rinaldo-Matthis,A. and Haeggstrom,J.Z.
  TITLE     Phosphorylation of Leukotriene C4 Synthase at Serine 36 Impairs
            Catalytic Activity
  JOURNAL   J Biol Chem 291 (35), 18410-18418 (2016)
   PUBMED   27365393
  REMARK    GeneRIF: mutational and kinetic data, together with molecular
            simulations, suggest that phosphorylation of Ser(36) inhibits the
            catalytic function of LTC4S by interference with the catalytic
            machinery.
REFERENCE   5  (bases 1 to 801)
  AUTHORS   Niegowski,D., Kleinschmidt,T., Olsson,U., Ahmad,S.,
            Rinaldo-Matthis,A. and Haeggstrom,J.Z.
  TITLE     Crystal structures of leukotriene C4 synthase in complex with
            product analogs: implications for the enzyme mechanism
  JOURNAL   J Biol Chem 289 (8), 5199-5207 (2014)
   PUBMED   24366866
  REMARK    GeneRIF: Biochemical and structural characterization of LTC4S
            mutants along with crystal structures of the wild type and mutated
            enzymes in complex with three product analogs, provide new insights
            to binding of substrates and product.
REFERENCE   6  (bases 1 to 801)
  AUTHORS   Penrose,J.F., Spector,J., Lam,B.K., Friend,D.S., Xu,K., Jack,R.M.
            and Austen,K.F.
  TITLE     Purification of human lung leukotriene C4 synthase and preparation
            of a polyclonal antibody
  JOURNAL   Am J Respir Crit Care Med 152 (1), 283-289 (1995)
   PUBMED   7599836
REFERENCE   7  (bases 1 to 801)
  AUTHORS   Soderstrom,M., Morgenstern,R. and Hammarstrom,S.
  TITLE     Protein-protein interaction affinity chromatography of leukotriene
            C4 synthase
  JOURNAL   Protein Expr Purif 6 (3), 352-356 (1995)
   PUBMED   7663172
REFERENCE   8  (bases 1 to 801)
  AUTHORS   Welsch,D.J., Creely,D.P., Hauser,S.D., Mathis,K.J., Krivi,G.G. and
            Isakson,P.C.
  TITLE     Molecular cloning and expression of human leukotriene-C4 synthase
  JOURNAL   Proc Natl Acad Sci U S A 91 (21), 9745-9749 (1994)
   PUBMED   7937884
REFERENCE   9  (bases 1 to 801)
  AUTHORS   Penrose,J.F., Gagnon,L., Goppelt-Struebe,M., Myers,P., Lam,B.K.,
            Jack,R.M., Austen,K.F. and Soberman,R.J.
  TITLE     Purification of human leukotriene C4 synthase
  JOURNAL   Proc Natl Acad Sci U S A 89 (23), 11603-11606 (1992)
   PUBMED   1454853
REFERENCE   10 (bases 1 to 801)
  AUTHORS   Nicholson,D.W., Ali,A., Klemba,M.W., Munday,N.A., Zamboni,R.J. and
            Ford-Hutchinson,A.W.
  TITLE     Human leukotriene C4 synthase expression in dimethyl
            sulfoxide-differentiated U937 cells
  JOURNAL   J Biol Chem 267 (25), 17849-17857 (1992)
   PUBMED   1517222
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC008393.7.
            This sequence is a reference standard in the RefSeqGene project.
            
            On May 17, 2019 this sequence version replaced NM_145867.1.
            
            Summary: The MAPEG (Membrane Associated Proteins in Eicosanoid and
            Glutathione metabolism) family includes a number of human proteins,
            several of which are involved the production of leukotrienes. This
            gene encodes an enzyme that catalyzes the first step in the
            biosynthesis of cysteinyl leukotrienes, potent biological compounds
            derived from arachidonic acid. Leukotrienes have been implicated as
            mediators of anaphylaxis and inflammatory conditions such as human
            bronchial asthma. This protein localizes to the nuclear envelope
            and adjacent endoplasmic reticulum. [provided by RefSeq, Jul 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC029498.1, BI668417.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968540, SAMEA1968832
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000292596.15/ ENSP00000292596.10
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-153               AC008393.7         53335-53487
            154-253             AC008393.7         54933-55032
            254-324             AC008393.7         55135-55205
            325-406             AC008393.7         55290-55371
            407-801             AC008393.7         55602-55996
FEATURES             Location/Qualifiers
     source          1..801
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="5"
                     /map="5q35.3"
     gene            1..801
                     /gene="LTC4S"
                     /note="leukotriene C4 synthase"
                     /db_xref="GeneID:4056"
                     /db_xref="HGNC:HGNC:6719"
                     /db_xref="MIM:246530"
     exon            1..153
                     /gene="LTC4S"
                     /inference="alignment:Splign:2.1.0"
     CDS             96..548
                     /gene="LTC4S"
                     /EC_number="4.4.1.20"
                     /note="LTC4 synthase; glutathione S-transferase LTC4"
                     /codon_start=1
                     /product="leukotriene C4 synthase"
                     /protein_id="NP_665874.1"
                     /db_xref="CCDS:CCDS34316.1"
                     /db_xref="GeneID:4056"
                     /db_xref="HGNC:HGNC:6719"
                     /db_xref="MIM:246530"
                     /translation="MKDEVALLAAVTLLGVLLQAYFSLQVISARRAFRVSPPLTTGPP
                     EFERVYRAQVNCSEYFPLFLATLWVAGIFFHEGAAALCGLVYLFARLRYFQGYARSAQ
                     LRLAPLYASARALWLLVALAALGLLAHFLPAALRAALLGRLRTLLPWA"
     exon            154..253
                     /gene="LTC4S"
                     /inference="alignment:Splign:2.1.0"
     exon            254..324
                     /gene="LTC4S"
                     /inference="alignment:Splign:2.1.0"
     exon            325..406
                     /gene="LTC4S"
                     /inference="alignment:Splign:2.1.0"
     exon            407..801
                     /gene="LTC4S"
                     /inference="alignment:Splign:2.1.0"
     regulatory      645..650
                     /regulatory_class="polyA_signal_sequence"
                     /gene="LTC4S"
                     /note="hexamer: ATTAAA"
     polyA_site      666
                     /gene="LTC4S"
                     /note="major polyA site"
ORIGIN      
        1 cttcctctcc tgggccgtcc tctgagcagc agacggggct aagcgttccc cagctcgcct
       61 tcacacacag cccgtgccac cacaccgacg gtaccatgaa ggacgaggta gctctactgg
      121 ctgctgtcac cctcctggga gtcctgctgc aagcctactt ctccctgcag gtgatctcgg
      181 cgcgcagggc cttccgcgtg tcgccgccgc tcaccaccgg cccacccgag ttcgagcgcg
      241 tctaccgagc ccaggtgaac tgcagcgagt acttcccgct gttcctcgcc acgctctggg
      301 tcgccggcat cttctttcat gaaggggcgg cggccctgtg cggcctggtc tacctgttcg
      361 cgcgcctccg ctacttccag ggctacgcgc gctccgcgca gctcaggctg gcaccgctgt
      421 acgcgagcgc gcgcgccctc tggctgctgg tggcgctggc tgcgctcggc ctgctcgccc
      481 acttcctccc ggccgcgctg cgcgccgcgc tcctcggacg gctccggacg ctgctgccgt
      541 gggcctgaga ccaaggcccc cgggccgacg gagccgggaa agaagagccg gagcctccag
      601 ctgccccggg gaggggcgct cgcttccgca tcctagtctc tatcattaaa gttctagtga
      661 ccgagacccg ggctgcgttc tctgggtccg cgggggtggc gcaccgcggg ctacggagcc
      721 tggaggggcc cagcccgagt ccgggcagcc cggggcgggc ttcctagtgg cggcgtgaga
      781 gtggctgcga aggaacgagc c
//
