LOCUS       NM_206943               6502 bp    mRNA    linear   PRI 12-OCT-2024
DEFINITION  Homo sapiens latent transforming growth factor beta binding protein
            1 (LTBP1), transcript variant 1, mRNA.
ACCESSION   NM_206943
VERSION     NM_206943.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6502)
  AUTHORS   Jiang,X., Yin,S., Yin,X., Wang,Y., Fang,T., Yang,S., Bian,X.,
            Li,G., Xue,Y. and Zhang,L.
  TITLE     A prognostic marker LTBP1 is associated with epithelial mesenchymal
            transition and can promote the progression of gastric cancer
  JOURNAL   Funct Integr Genomics 24 (1), 30 (2024)
   PUBMED   38358412
  REMARK    GeneRIF: A prognostic marker LTBP1 is associated with epithelial
            mesenchymal transition and can promote the progression of gastric
            cancer.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6502)
  AUTHORS   Wenk,D., Khan,S., Ignatchenko,V., May,T., Bernardini,M.Q. and
            Kislinger,T.
  TITLE     Targeted Mass Spectrometry of Longitudinal Patient Sera Reveals
            LTBP1 as a Potential Surveillance Biomarker for High-Grade Serous
            Ovarian Carcinoma
  JOURNAL   J Proteome Res 23 (2), 749-759 (2024)
   PUBMED   38266179
  REMARK    GeneRIF: Targeted Mass Spectrometry of Longitudinal Patient Sera
            Reveals LTBP1 as a Potential Surveillance Biomarker for High-Grade
            Serous Ovarian Carcinoma.
REFERENCE   3  (bases 1 to 6502)
  AUTHORS   Gu,H., Wang,W., Sun,C., Ding,L., Li,L., Shu,P. and Xu,J.
  TITLE     Immune suppressive signaling regulated by latent transforming
            growth factor beta binding protein 1 promotes metastasis in
            cervical cancer
  JOURNAL   Braz J Med Biol Res 55, e12206 (2023)
   PUBMED   36629522
  REMARK    GeneRIF: Immune suppressive signaling regulated by latent
            transforming growth factor beta binding protein 1 promotes
            metastasis in cervical cancer.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 6502)
  AUTHORS   Przyklenk,M., Georgieva,V.S., Metzen,F., Mostert,S., Kobbe,B.,
            Callewaert,B., Sengle,G., Brachvogel,B., Mecham,R.P., Paulsson,M.,
            Wagener,R., Koch,M. and Schiavinato,A.
  TITLE     LTBP1 promotes fibrillin incorporation into the extracellular
            matrix
  JOURNAL   Matrix Biol 110, 60-75 (2022)
   PUBMED   35452817
  REMARK    GeneRIF: LTBP1 promotes fibrillin incorporation into the
            extracellular matrix.
REFERENCE   5  (bases 1 to 6502)
  AUTHORS   Taipale,J., Saharinen,J., Hedman,K. and Keski-Oja,J.
  TITLE     Latent transforming growth factor-beta 1 and its binding protein
            are components of extracellular matrix microfibrils
  JOURNAL   J Histochem Cytochem 44 (8), 875-889 (1996)
   PUBMED   8756760
REFERENCE   6  (bases 1 to 6502)
  AUTHORS   Saharinen,J., Taipale,J. and Keski-Oja,J.
  TITLE     Association of the small latent transforming growth factor-beta
            with an eight cysteine repeat of its binding protein LTBP-1
  JOURNAL   EMBO J 15 (2), 245-253 (1996)
   PUBMED   8617200
REFERENCE   7  (bases 1 to 6502)
  AUTHORS   Olofsson,A., Ichijo,H., Moren,A., ten Dijke,P., Miyazono,K. and
            Heldin,C.H.
  TITLE     Efficient association of an amino-terminally extended form of human
            latent transforming growth factor-beta binding protein with the
            extracellular matrix
  JOURNAL   J Biol Chem 270 (52), 31294-31297 (1995)
   PUBMED   8537398
REFERENCE   8  (bases 1 to 6502)
  AUTHORS   Stenman,G., Sahlin,P., Olofsson,A., Geurts van Kessel,A. and
            Miyazono,K.
  TITLE     Assignment of the gene encoding the latent TGF-beta 1-binding
            protein (LTBP1) to human chromosome 2, region p12-->q22
  JOURNAL   Cytogenet Cell Genet 66 (2), 117-119 (1994)
   PUBMED   8287682
REFERENCE   9  (bases 1 to 6502)
  AUTHORS   Miyazono,K., Olofsson,A., Colosetti,P. and Heldin,C.H.
  TITLE     A role of the latent TGF-beta 1-binding protein in the assembly and
            secretion of TGF-beta 1
  JOURNAL   EMBO J 10 (5), 1091-1101 (1991)
   PUBMED   2022183
REFERENCE   10 (bases 1 to 6502)
  AUTHORS   Kanzaki,T., Olofsson,A., Moren,A., Wernstedt,C., Hellman,U.,
            Miyazono,K., Claesson-Welsh,L. and Heldin,C.H.
  TITLE     TGF-beta 1 binding protein: a component of the large latent complex
            of TGF-beta 1 with multiple repeat sequences
  JOURNAL   Cell 61 (6), 1051-1061 (1990)
   PUBMED   2350783
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL133244.1, AC019195.10,
            AC019127.10 and AC020594.6.
            
            On May 21, 2020 this sequence version replaced NM_206943.3.
            
            Summary: The protein encoded by this gene belongs to the family of
            latent TGF-beta binding proteins (LTBPs). The secretion and
            activation of TGF-betas is regulated by their association with
            latency-associated proteins and with latent TGF-beta binding
            proteins. The product of this gene targets latent complexes of
            transforming growth factor beta to the extracellular matrix, where
            the latent cytokine is subsequently activated by several different
            mechanisms. Alternatively spliced transcript variants encoding
            different isoforms have been identified. [provided by RefSeq, Jul
            2008].
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (LTBP-1L). There are no
            full-length transcripts representing this variant in human, but it
            is supported by overlapping partial transcripts, full-length
            homologous rat and mouse transcripts, and data in PMID:8537398.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR11853561.15754.1,
                                           SRR14038192.193946.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1966682, SAMEA1968189
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000404816.7/ ENSP00000386043.2
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-866               AL133244.1         17183-18048
            867-937             AL133244.1         19105-19175
            938-1235            AL133244.1         91139-91436
            1236-1405           AL133244.1         180812-180981
            1406-1573           AC019195.10        138996-139163       c
            1574-1798           AC019195.10        86876-87100         c
            1799-2073           AC019195.10        85105-85379         c
            2074-2176           AC019195.10        56302-56404         c
            2177-2248           AC019195.10        51805-51876         c
            2249-2371           AC019195.10        30172-30294         c
            2372-2539           AC019195.10        21112-21279         c
            2540-2767           AC019195.10        16445-16672         c
            2768-2790           AC019195.10        14346-14368         c
            2791-2890           AC019195.10        11135-11234         c
            2891-2989           AC019195.10        10564-10662         c
            2990-3115           AC019127.10        1701-1826
            3116-3241           AC019127.10        3010-3135
            3242-3364           AC019127.10        3846-3968
            3365-3484           AC019127.10        8084-8203
            3485-3607           AC019127.10        21205-21327
            3608-3730           AC019127.10        28496-28618
            3731-3853           AC019127.10        29567-29689
            3854-3976           AC019127.10        37479-37601
            3977-4102           AC019127.10        43189-43314
            4103-4228           AC019127.10        70883-71008
            4229-4372           AC019127.10        75412-75555
            4373-4555           AC019127.10        88642-88824
            4556-4642           AC019127.10        89474-89560
            4643-4771           AC019127.10        91435-91563
            4772-4912           AC019127.10        92261-92401
            4913-5083           AC019127.10        93378-93548
            5084-5206           AC020594.6         14111-14233
            5207-5356           AC020594.6         22060-22209
            5357-6502           AC020594.6         23291-24436
FEATURES             Location/Qualifiers
     source          1..6502
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="2"
                     /map="2p22.3"
     gene            1..6502
                     /gene="LTBP1"
                     /gene_synonym="ARCL2E"
                     /note="latent transforming growth factor beta binding
                     protein 1"
                     /db_xref="GeneID:4052"
                     /db_xref="HGNC:HGNC:6714"
                     /db_xref="MIM:150390"
     exon            1..866
                     /gene="LTBP1"
                     /gene_synonym="ARCL2E"
                     /inference="alignment:Splign:2.1.0"
     CDS             373..5538
                     /gene="LTBP1"
                     /gene_synonym="ARCL2E"
                     /note="isoform LTBP-1L precursor is encoded by transcript
                     variant 1; TGF-beta1-BP-1; transforming growth factor
                     beta-1-binding protein 1"
                     /codon_start=1
                     /product="latent-transforming growth factor beta-binding
                     protein 1 isoform LTBP-1L precursor"
                     /protein_id="NP_996826.3"
                     /db_xref="CCDS:CCDS33177.2"
                     /db_xref="GeneID:4052"
                     /db_xref="HGNC:HGNC:6714"
                     /db_xref="MIM:150390"
                     /translation="MAGAWLRWGLLLWAGLLASSAHGRLRRITYVVHPGPGLAAGALP
                     LSGPPRSRTFNVALNARYSRSSAAAGAPSRASPGVPSERTRRTSKPGGAALQGLRPPP
                     PPPPEPARPAVPGGQLHPNPGGHPAAAPFTKQGRQVVRSKVPQETQSGGGSRLQVHQK
                     QQLQGVNVCGGRCCHGWSKAPGSQRCTKPSCVPPCQNGGMCLRPQLCVCKPGTKGKAC
                     ETIAAQDTSSPVFGGQSPGAASSWGPPEQAAKHTSSKKADTLPRVSPVAQMTLTLKPK
                     PSVGLPQQIHSQVTPLSSQSVVIHHGQTQEYVLKPKYFPAQKGISGEQSTEGSFPLRY
                     VQDQVAAPFQLSNHTGRIKVVFTPSICKVTCTKGSCQNSCEKGNTTTLISENGHAADT
                     LTATNFRVVICHLPCMNGGQCSSRDKCQCPPNFTGKLCQIPVHGASVPKLYQHSQQPG
                     KALGTHVIHSTHTLPLTVTSQQGVKVKFPPNIVNIHVKHPPEASVQIHQVSRIDGPTG
                     QKTKEAQPGQSQVSYQGLPVQKTQTIHSTYSHQQVIPHVYPVAAKTQLGRCFQETIGS
                     QCGKALPGLSKQEDCCGTVGTSWGFNKCQKCPKKPSYHGYNQMMECLPGYKRVNNTFC
                     QDINECQLQGVCPNGECLNTMGSYRCTCKIGFGPDPTFSSCVPDPPVISEEKGPCYRL
                     VSSGRQCMHPLSVHLTKQLCCCSVGKAWGPHCEKCPLPGTAAFKEICPGGMGYTVSGV
                     HRRRPIHHHVGKGPVFVKPKNTQPVAKSTHPPPLPAKEEPVEALTFSREHGPGVAEPE
                     VATAPPEKEIPSLDQEKTKLEPGQPQLSPGISTIHLHPQFPVVIEKTSPPVPVEVAPE
                     ASTSSASQVIAPTQVTEINECTVNPDICGAGHCINLPVRYTCICYEGYRFSEQQRKCV
                     DIDECTQVQHLCSQGRCENTEGSFLCICPAGFMASEEGTNCIDVDECLRPDVCGEGHC
                     VNTVGAFRCEYCDSGYRMTQRGRCEDIDECLNPSTCPDEQCVNSPGSYQCVPCTEGFR
                     GWNGQCLDVDECLEPNVCANGDCSNLEGSYMCSCHKGYTRTPDHKHCRDIDECQQGNL
                     CVNGQCKNTEGSFRCTCGQGYQLSAAKDQCEDIDECQHRHLCAHGQCRNTEGSFQCVC
                     DQGYRASGLGDHCEDINECLEDKSVCQRGDCINTAGSYDCTCPDGFQLDDNKTCQDIN
                     ECEHPGLCGPQGECLNTEGSFHCVCQQGFSISADGRTCEDIDECVNNTVCDSHGFCDN
                     TAGSFRCLCYQGFQAPQDGQGCVDVNECELLSGVCGEAFCENVEGSFLCVCADENQEY
                     SPMTGQCRSRTSTDLDVDVDQPKEEKKECYYNLNDASLCDNVLAPNVTKQECCCTSGV
                     GWGDNCEIFPCPVLGTAEFTEMCPKGKGFVPAGESSSEAGGENYKDADECLLFGQEIC
                     KNGFCLNTRPGYECYCKQGTYYDPVKLQCFDMDECQDPSSCIDGQCVNTEGSYNCFCT
                     HPMVLDASEKRCIRPAESNEQIEETDVYQDLCWEHLSDEYVCSRPLVGKQTTYTECCC
                     LYGEAWGMQCALCPLKDSDDYAQLCNIPVTGRRQPYGRDALVDFSEQYTPEADPYFIQ
                     DRFLNSFEELQAEECGILNGCENGRCVRVQEGYTCDCFDGYHLDTAKMTCVDVNECDE
                     LNNRMSLCKNAKCINTDGSYKCLCLPGYVPSDKPNYCTPLNTALNLEKDSDLE"
     exon            867..937
                     /gene="LTBP1"
                     /gene_synonym="ARCL2E"
                     /inference="alignment:Splign:2.1.0"
     exon            938..1235
                     /gene="LTBP1"
                     /gene_synonym="ARCL2E"
                     /inference="alignment:Splign:2.1.0"
     exon            1236..1405
                     /gene="LTBP1"
                     /gene_synonym="ARCL2E"
                     /inference="alignment:Splign:2.1.0"
     exon            1406..1573
                     /gene="LTBP1"
                     /gene_synonym="ARCL2E"
                     /inference="alignment:Splign:2.1.0"
     exon            1574..1798
                     /gene="LTBP1"
                     /gene_synonym="ARCL2E"
                     /inference="alignment:Splign:2.1.0"
     exon            1799..2073
                     /gene="LTBP1"
                     /gene_synonym="ARCL2E"
                     /inference="alignment:Splign:2.1.0"
     exon            2074..2176
                     /gene="LTBP1"
                     /gene_synonym="ARCL2E"
                     /inference="alignment:Splign:2.1.0"
     exon            2177..2248
                     /gene="LTBP1"
                     /gene_synonym="ARCL2E"
                     /inference="alignment:Splign:2.1.0"
     exon            2249..2371
                     /gene="LTBP1"
                     /gene_synonym="ARCL2E"
                     /inference="alignment:Splign:2.1.0"
     exon            2372..2539
                     /gene="LTBP1"
                     /gene_synonym="ARCL2E"
                     /inference="alignment:Splign:2.1.0"
     exon            2540..2767
                     /gene="LTBP1"
                     /gene_synonym="ARCL2E"
                     /inference="alignment:Splign:2.1.0"
     exon            2768..2790
                     /gene="LTBP1"
                     /gene_synonym="ARCL2E"
                     /inference="alignment:Splign:2.1.0"
     exon            2791..2890
                     /gene="LTBP1"
                     /gene_synonym="ARCL2E"
                     /inference="alignment:Splign:2.1.0"
     exon            2891..2989
                     /gene="LTBP1"
                     /gene_synonym="ARCL2E"
                     /inference="alignment:Splign:2.1.0"
     exon            2990..3115
                     /gene="LTBP1"
                     /gene_synonym="ARCL2E"
                     /inference="alignment:Splign:2.1.0"
     exon            3116..3241
                     /gene="LTBP1"
                     /gene_synonym="ARCL2E"
                     /inference="alignment:Splign:2.1.0"
     exon            3242..3364
                     /gene="LTBP1"
                     /gene_synonym="ARCL2E"
                     /inference="alignment:Splign:2.1.0"
     exon            3365..3484
                     /gene="LTBP1"
                     /gene_synonym="ARCL2E"
                     /inference="alignment:Splign:2.1.0"
     exon            3485..3607
                     /gene="LTBP1"
                     /gene_synonym="ARCL2E"
                     /inference="alignment:Splign:2.1.0"
     exon            3608..3730
                     /gene="LTBP1"
                     /gene_synonym="ARCL2E"
                     /inference="alignment:Splign:2.1.0"
     exon            3731..3853
                     /gene="LTBP1"
                     /gene_synonym="ARCL2E"
                     /inference="alignment:Splign:2.1.0"
     exon            3854..3976
                     /gene="LTBP1"
                     /gene_synonym="ARCL2E"
                     /inference="alignment:Splign:2.1.0"
     exon            3977..4102
                     /gene="LTBP1"
                     /gene_synonym="ARCL2E"
                     /inference="alignment:Splign:2.1.0"
     exon            4103..4228
                     /gene="LTBP1"
                     /gene_synonym="ARCL2E"
                     /inference="alignment:Splign:2.1.0"
     exon            4229..4372
                     /gene="LTBP1"
                     /gene_synonym="ARCL2E"
                     /inference="alignment:Splign:2.1.0"
     exon            4373..4555
                     /gene="LTBP1"
                     /gene_synonym="ARCL2E"
                     /inference="alignment:Splign:2.1.0"
     exon            4556..4642
                     /gene="LTBP1"
                     /gene_synonym="ARCL2E"
                     /inference="alignment:Splign:2.1.0"
     exon            4643..4771
                     /gene="LTBP1"
                     /gene_synonym="ARCL2E"
                     /inference="alignment:Splign:2.1.0"
     exon            4772..4912
                     /gene="LTBP1"
                     /gene_synonym="ARCL2E"
                     /inference="alignment:Splign:2.1.0"
     exon            4913..5083
                     /gene="LTBP1"
                     /gene_synonym="ARCL2E"
                     /inference="alignment:Splign:2.1.0"
     exon            5084..5206
                     /gene="LTBP1"
                     /gene_synonym="ARCL2E"
                     /inference="alignment:Splign:2.1.0"
     exon            5207..5356
                     /gene="LTBP1"
                     /gene_synonym="ARCL2E"
                     /inference="alignment:Splign:2.1.0"
     exon            5357..6502
                     /gene="LTBP1"
                     /gene_synonym="ARCL2E"
                     /inference="alignment:Splign:2.1.0"
     regulatory      6330..6335
                     /regulatory_class="polyA_signal_sequence"
                     /gene="LTBP1"
                     /gene_synonym="ARCL2E"
                     /note="hexamer: AATAAA"
     polyA_site      6356
                     /gene="LTBP1"
                     /gene_synonym="ARCL2E"
                     /note="major polyA site"
     regulatory      6471..6476
                     /regulatory_class="polyA_signal_sequence"
                     /gene="LTBP1"
                     /gene_synonym="ARCL2E"
                     /note="hexamer: ATTAAA"
     polyA_site      6502
                     /gene="LTBP1"
                     /gene_synonym="ARCL2E"
ORIGIN      
        1 ggagtgtccc gcgcgctctc gctcgctctc ggccaccctc gccgggcccc gctgcggcgc
       61 gcgctgcaac ccccggccgg acgcgcggac cctcaccttg cgcggcccgc tcccctcgcc
      121 cctccccgct ccccgggctc cgcgctcccc acccccacgc cctcctcctg ctcccagcca
      181 caatcggccg gggtctgggg ccgctcagct gcccgcagag cctcctccct cgccaccgac
      241 ttggtctcct cccgcctttc ccgggctctc ggcagctctc gggggagccc gaacgcgcgg
      301 ggaaaggcga gccgcacggc cgggggaggg ggccggaccg cgcgcgaccg gtcgcgcccg
      361 ctggggcccg cgatggcggg ggcctggctc aggtgggggc tcctgctctg ggcagggctc
      421 ctcgcgtcct cggcgcacgg ccggctgcgg aggatcacct acgtggtgca cccgggcccc
      481 ggcctggcag ccggcgcctt gcccctgagc gggcccccgc gttcgcggac attcaacgtc
      541 gcgctcaacg ccaggtacag ccgcagctcg gcggctgccg gcgcccccag ccgtgcctcc
      601 cccggggtcc cctcggagag gacccggcgc acgagcaagc cgggcggcgc ggccctgcag
      661 gggctcagac cgccgccgcc gccgccgccg gagcctgcgc gtcccgcggt ccccggcggg
      721 cagctccacc ccaatcccgg cggccacccg gcagccgccc cgttcaccaa acaaggcagg
      781 caagttgtgc gctccaaggt gccgcaggag acccagagcg gcggaggctc taggctgcag
      841 gttcaccaga agcagcagct gcagggggtc aatgtctgtg gagggcggtg ctgtcatggc
      901 tggagtaagg cccctggctc ccagaggtgc accaaaccta gctgtgttcc gccatgtcag
      961 aatggaggga tgtgtctccg gccacaactc tgtgtgtgta aaccagggac caagggcaaa
     1021 gcctgtgaaa caatagctgc ccaggacacc tcgtcaccag tctttggagg gcagagtcct
     1081 ggggctgctt cctcgtgggg ccctcctgag caagcagcaa agcatacttc atctaagaag
     1141 gcagacactc taccaagagt cagccctgtg gcccagatga ccttaaccct caagccgaag
     1201 ccttcagtgg gactccccca gcagatacat tctcaagtga ctcctctttc ttcccagagt
     1261 gtggtgattc accatggcca gacccaggaa tacgtgctca agcccaagta ctttccagcc
     1321 cagaagggga tttcaggaga gcagtccact gaaggttctt tccctttaag atatgtgcag
     1381 gatcaagttg cggcaccttt tcagctgagt aaccacactg gccgcatcaa ggtggtcttt
     1441 actccgagca tctgtaaagt gacctgcacc aagggcagct gtcagaacag ctgtgagaag
     1501 gggaacacca ccactctcat tagtgagaat ggtcatgctg ccgacaccct gacggccacg
     1561 aacttccgag tggtaatttg ccatcttcca tgtatgaatg gtggccagtg cagttcaagg
     1621 gacaaatgtc agtgccctcc aaatttcaca ggaaaacttt gtcagatccc agtccatggt
     1681 gccagcgtgc ctaaacttta tcagcattcc cagcagccag gcaaggcgtt ggggacgcat
     1741 gtcatccatt caacacatac cttgcctctg accgtgacta gccagcaagg agtcaaagtg
     1801 aaatttcctc ctaacatagt caatatccat gtgaaacatc ctcctgaagc ttccgtccag
     1861 atacatcagg tttcaagaat tgatggccca acaggccaga agacaaaaga agctcaacca
     1921 ggccaatccc aagtctcgta ccaagggctt cctgtccaga agacccagac catacattcc
     1981 acatactccc accagcaggt cattcctcac gtctaccccg tggctgctaa gacacagctt
     2041 ggccggtgct tccaggaaac cattgggtca cagtgtggca aagcgctccc tggcctttca
     2101 aagcaagagg actgctgtgg aactgtgggt acctcctggg gctttaacaa atgccagaaa
     2161 tgccccaaga aaccatctta tcatggatac aaccaaatga tggaatgcct accgggttat
     2221 aagcgggtta acaacacctt ttgccaagat attaatgaat gtcagctaca aggtgtatgc
     2281 cctaatggtg agtgtttgaa taccatgggc agctatcgat gtacctgcaa aataggattt
     2341 gggccggatc ctaccttttc aagttgtgtt cctgatcccc ctgtgatctc ggaagagaaa
     2401 gggccctgtt accgacttgt cagttctgga agacagtgta tgcaccctct gtctgttcac
     2461 ctcaccaagc agctctgctg ttgtagtgtg ggcaaggcct ggggcccaca ctgtgagaaa
     2521 tgtccccttc caggcacagc tgcttttaag gaaatctgtc ctggtggaat gggttatacg
     2581 gtttctggcg ttcatagacg caggccaatc catcaccatg taggtaaagg acctgtattt
     2641 gtcaagccaa agaacactca acctgttgct aaaagtactc atcctccacc tctcccagcc
     2701 aaggaagagc cagtggaggc cctgaccttc tcccgggaac acgggccagg agtggcggag
     2761 ccagaagtgg caactgcacc ccctgaaaag gaaatacctt cattggatca agagaaaacc
     2821 aaacttgagc ctggtcaacc ccagctgtct ccaggcattt ccactattca tctgcatcca
     2881 cagtttccag tagtgattga aaaaacatca cctcctgtgc ctgttgaagt agctcctgaa
     2941 gcttctacgt ctagtgccag ccaagtgatt gctcctactc aagtgacaga aatcaatgaa
     3001 tgtactgtga accctgatat ctgtggagca ggacactgca ttaacctacc agtgagatat
     3061 acctgtatat gctacgaggg ctacaggttc agtgaacaac agaggaaatg tgtggatatt
     3121 gatgagtgta ctcaggtcca acacctctgc tcccagggcc gctgtgaaaa caccgaggga
     3181 agtttcttgt gcatttgccc agcaggattt atggccagtg aggagggtac taactgcata
     3241 gatgttgacg aatgcctgag gccggacgtc tgtggggagg ggcactgtgt caatactgtg
     3301 ggggccttcc ggtgtgaata ctgtgacagc gggtaccgca tgactcagag aggccgttgt
     3361 gaggatattg atgaatgttt gaatccaagc acttgtccag atgagcagtg tgtgaattct
     3421 cctggatctt accagtgcgt tccctgcaca gaaggattcc gaggctggaa tggacagtgc
     3481 cttgatgtgg acgagtgcct ggaaccaaac gtctgcgcaa atggtgattg ttccaacctt
     3541 gaaggctcct acatgtgttc atgccacaaa ggctataccc ggactccgga ccacaagcac
     3601 tgtagagata ttgatgaatg tcagcaaggg aatctatgtg taaacgggca gtgcaaaaat
     3661 accgagggct ccttcaggtg cacctgtgga caggggtacc agctgtcggc agctaaagac
     3721 cagtgtgaag acattgatga atgccagcac cgtcatctct gtgctcatgg gcagtgcagg
     3781 aacactgagg gctcttttca atgtgtgtgt gaccagggtt acagagcatc tgggcttgga
     3841 gaccactgtg aagatatcaa tgaatgcttg gaggacaaga gtgtttgcca gagaggagac
     3901 tgcattaata ctgcagggtc ctatgattgt acttgtccgg atggatttca gctagatgac
     3961 aataaaacat gtcaagatat taatgaatgt gaacatccag ggctctgtgg tccgcaaggg
     4021 gagtgcctaa acacagaggg ttctttccat tgtgtctgcc agcagggttt ctcaatctct
     4081 gcagatggcc gtacgtgtga agatattgat gaatgtgtaa acaacactgt ttgtgacagt
     4141 cacgggtttt gtgacaatac agctggctcc ttccgctgcc tctgttatca gggctttcaa
     4201 gccccacagg atgggcaagg gtgtgtggat gtgaatgaat gtgaactgct cagtggggtg
     4261 tgtggtgaag ccttctgtga aaacgtggaa gggtccttcc tgtgcgtgtg tgctgatgaa
     4321 aaccaagagt acagccccat gactgggcag tgccgctccc ggacctccac agatttagat
     4381 gtagatgtag atcaacccaa agaagaaaag aaagaatgct actataatct caatgacgcc
     4441 agtctctgtg ataatgtgtt ggcccccaat gtcacgaaac aagaatgctg ctgtacatca
     4501 ggcgtgggat ggggagataa ctgcgaaatc ttcccctgcc cggtcttggg aactgctgag
     4561 ttcactgaaa tgtgtcccaa agggaaaggt tttgtgcctg ctggagaatc atcttctgaa
     4621 gctggtggtg agaactataa agatgcagat gaatgcctac tttttggaca agaaatctgc
     4681 aaaaatggtt tctgtttgaa cactcggcct gggtatgaat gctactgtaa gcaagggacg
     4741 tactatgatc ctgtgaaact gcagtgcttt gatatggatg aatgtcaaga ccccagtagt
     4801 tgtattgatg gccagtgtgt taatacagag ggctcttaca actgcttctg tactcacccc
     4861 atggtcctgg atgcgtcaga aaaaagatgt atacgaccgg ctgagtcaaa cgaacaaata
     4921 gaagaaactg atgtctacca agatttgtgc tgggaacatc tgagtgatga atacgtgtgt
     4981 agccggcctc ttgtgggcaa gcagacaacg tacactgagt gctgctgtct gtatggagag
     5041 gcctggggca tgcagtgtgc cctctgcccc ctgaaggatt cagatgacta tgctcagctg
     5101 tgtaacatcc ccgtgacggg acgccggcag ccatatggac gggacgcctt ggttgacttc
     5161 agtgaacagt atactccaga agccgatccc tacttcatcc aagaccgttt tctaaatagc
     5221 tttgaggagt tacaggctga ggaatgcggc atcctcaatg gatgtgaaaa tggtcgctgt
     5281 gtgagggtcc aggaaggtta cacctgcgat tgctttgatg ggtatcactt ggatacggcc
     5341 aagatgacct gtgtcgatgt aaatgaatgc gatgagttga acaaccggat gtctctctgc
     5401 aagaatgcca agtgcattaa caccgatggt tcctacaagt gtttgtgtct gccaggctac
     5461 gtgccttctg acaagccaaa ctactgcact ccgttgaata ccgccttgaa tttagagaaa
     5521 gacagtgacc tggagtgaaa cagaatctac ataacctaag cccatatact ctgcactgtg
     5581 taaaggaaaa gggagaaatg tattatactt gagacattgc acctaccccg gaaggctgga
     5641 aatacagaaa cagcatggaa ttgcaagtcc tctgaagaca atgagaggat ttaggatgag
     5701 cccgataggt gtggcagacc aaatggacat ttctctaaaa aaccagtata tatagtctgt
     5761 tcatatgtaa aattcaatgg aagagaggtg gaacagtgct gttattttaa acagaaggtt
     5821 gtattattat gttgttttgt ttttttacta ttgcttgatt aaatttggca tttaaatagt
     5881 ggtggaaata ttttatataa ttttcatttt ttggttgtgc agttccttgg ctactgtttt
     5941 tcttttactt cagtttttta aaaatctcaa atgaaaaagt cttcgataca atattgttaa
     6001 gctgtattat aagtattgtt acacagggtt atgcaattcc cggcctggag catttttgaa
     6061 attcaaattg tctgtcctgt ggagcaggca gtgattttgt tccaaaactt tgtatacaca
     6121 tttggagaaa agtactttat attttcagtg ttttgtctga ttttaatgtc cgttcttagc
     6181 caagctgcta gcaggtgtta attggatccc tttccttcac tgaaatggaa gagtttataa
     6241 gcttacgtta gtattgtaat atgtaaagta agcccaacaa aaatttttaa aaatttgatg
     6301 atccccaata tatctaccat tgtatgttaa ataaatcacc atttttgtag aaaaaattct
     6361 acctgagagt aattgtcaat gagtacatgt gtataagttg tatcccactc tccccacttt
     6421 tatcttttcc agtggtcttc tgttaatgta gtgtctttta caagttaatc attaaatttg
     6481 ttagatcttg ttatgggcta aa
//
